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Antisense Oligonucleotides-Mediated Rescue Of The Amelx Splicing Defects, Eui-Sic Cho, Youn Jung Kim, Figen Seymen, Mine Koruyucu, Jan C.-C. Hu, James P. Simmer, Jung-Wook Kim

Journal of Dental Sciences

Background/purpose: Amelogenesis imperfecta (AI) is a group of rare, inherited disorders characterized by abnormal enamel formation. While identifying the genes and mutations that cause the disease is important, understanding its molecular pathogenesis is essential to developing precision and personalized medicine, which aim to stop disease onset, slow its progression, and provide a range of treatment options. Previously, we identified mutations that affect the conserved alternative splicing of the AMELX gene, leading to the inclusion of normally skipped exon 4 and resulting in a characteristic AI phenotype.

Materials and methods: HEK293 cells were grown on cell culture dishes …

Biomechanical Effects Of Titanium Alloy Removable Partial Denture Framework Designs Fabricated By Selective Laser Melting In Mandibular Kennedy Class Ii Partial Edentulism: A Finite Element Study, Min-Chieh Chang, Ping-Ching Wu, Wei-Shao Lin, Yung-Chung Chen

Journal of Dental Sciences

Background/purpose: The biomechanical effects of framework design and material interactions in mandibular free-end saddle removable partial dentures remain unclear. This study evaluated framework variables using finite element analysis.

Materials and methods: A mandibular Kennedy Class II model was digitized to create a cobalt-chromium (CoCr) and sixteen Ti-6Al-4V (Ti64) frameworks varying by major connector dimension, clasp configuration, and lingual reciprocal assembly (LRA). Periodontal ligament (PDL) and mucosa layers were incorporated. Contacts were defined, and materials were assumed homogeneous and isotropic. Vertical and oblique loads were applied, and von Mises stresses were analyzed. Model validation was performed using digital image correlation. …

N-Acetylcysteine Restores Osteogenesis In Human Periodontal Ligament Stem Cells By Attenuating Hif-1Α-Driven Oxidative Stress And Glycolytic Reprogramming, Xiaochi Chang, Yanghao Zhao, Yang Wang, Chang Shu, Yi Feng, Yichong Sun, Guangxun Zhu, Jianxia Hou

Journal of Dental Sciences

Background/purpose: Periodontitis-associated hypoxia signaling and oxidative stress severely impair the regenerative capacity of human periodontal ligament stem cells (hPDLSCs). This study investigated whether antioxidant intervention rescues hPDLSC osteogenesis under CoCl₂-induced HIF-1α stabilization by modulating PI3K/Akt/HIF-1α/HO-1 signaling and glycolytic reprogramming.

Materials and methods: Primary hPDLSCs isolated from healthy premolars were characterized by flow cytometry and multilineage differentiation. To simulate periodontitis-associated stress, a CoCl₂-induced HIF-1α-stabilizing condition was established. Cells were treated with the antioxidant N-acetylcysteine (NAC), the glycolysis inhibitor 2-deoxy-D-glucose (2-DG), or the PI3K inhibitor LY294002. Redox balance (DCFH-DA, MDA, GPX activity), glycolysis-related markers (HK2, GLUT1, lactate), and osteogenic-related markers …

Quantitative Cbct Assessment Of Anterior Loop Safety Margins Using Canine-Guided Osteotomy Designs For Chin Bone Harvesting., Han-Sheng Chen, Kun-Jung Hsu, Hsiu-Yueh Liu, Hui-Na Lee, Chun-Ming Chen

Journal of Dental Sciences

Background/purpose: Chin bone harvesting is a common intraoral grafting procedure but carries a risk of neurosensory complications involving the mental foramen (MF), anterior loop (AL), and mandibular incisive canal (MIC). Present study quantitatively evaluated whether the mandibular canine can serve as a safer and more reproducible landmark for chin bone harvesting.

Materials and methods: Cone-beam computed tomography (CBCT) scans from 40 adults were analyzed. The mandibular canine distal angle (CDA) and canine long axis (CLA) were used as reference lines. Measurements included AL-MF length, distances from the CLA to the MF and AL, interMF distance (IntMF), interAL distance (IntAL), interCLA …

Performance Of Chatgpt-4, Gemini, And Deepseek-V3 On English-Translated Questions From The Taiwan National Dental Technician Licensing Examination Over A Three-Week Period, Yi-Pang Lee, Ching-Yi Huang, Andy Sun, Chun-Pin Chiang

Journal of Dental Sciences

Background/purpose: Large language models (LLMs) have shown potential in answering professional examination questions. This study evaluated the performance of ChatGPT-4, Gemini, and DeepSeek-V3 in answering English-translated questions from the 2023 Taiwan National Dental Technician Licensing Examination (TNDTLE) over a three-week period.

Materials and methods: A total of 194 English-translated, text-based multiple-choice questions were selected from the 2023 TNDTLE. ChatGPT-4, Gemini, and DeepSeek-V3 were used to answer the same set of English-translated questions at four time points: baseline and one-, two-, and three-week follow-ups. Accuracy rates (ARs) were calculated and compared to evaluate changes over time and differences among the three …

A Comprehensive Analysis And Sex-Related Differences In Root And Root Canal Anatomy In The Taiwanese Population, Ho-Sheng Chiang, Wei-Cheng Lee, Ying-Wu Chen, Ling-Yu Kung, Kuei-Yuan Chen, Ren-Yeong Huang, Yi-Shing Shieh, Yu-Chiao Wu

Journal of Dental Sciences

Background/purpose: Root canal morphology exhibits substantial anatomical variation influenced by ethnicity and sex, which may complicate endodontic treatment if unrecognized. This study aimed to comprehensively evaluate root and root canal configurations in a Taiwanese population using cone-beam computed tomography (CBCT) and to investigate sex-related differences in canal anatomy.

Materials and methods: CBCT scans from 802 patients treated between 2022 and 2024 were retrospectively analyzed. A total of 18,578 teeth met the inclusion criteria. Root canal configurations were assessed for every root using Vertucci’s classification, and C-shaped canals were further categorized based on morphology. Sex-related differences were analyzed using chi-square tests. …

Performance Of Chatgpt-4, Gemini, And Deepseek-V3 In Answering The Prompt-Engineered Questions From The Taiwan National Dental Technician Licensing Examination Over A Three-Week Period, Yi-Pang Lee, Ching-Yi Huang, Andy Sun, Chun-Pin Chiang

Journal of Dental Sciences

Background/purpose: Large language models (LLMs) have shown promise in answering professional examination questions. This study evaluated the performance of ChatGPT-4, Gemini, and DeepSeek-V3 in answering prompt-engineered questions selected from the 2023 Taiwan National Dental Technician Licensing Examination (TNDTLE) over a three-week period.

Materials and methods: A total of 194 Chinese-language text-based multiple-choice questions were selected from the 2023 TNDTLE. The three LLMs answered these questions using a standardized prompt referring to 17 dental technology textbooks. Accuracy rates (ARs) were recorded at baseline and after one, two, and three weeks. Comparisons of ARs over time, among three LLMs, between basic and …

Ensemble Learning For Predicting Periapical Lesions From Dental Radiographs, Fang-Yuan Liao, Ming-Jyun Jian, Abdullah Sarini, Yulianto Jesica, Yen-Kun Lin, Hsun-Yu Huang

Journal of Dental Sciences

Background/purpose: Diagnosing periapical cysts and granulomas using periapical radiographs is challenging due to subtle radiographic differences. This study aimed to develop a machine learning–based diagnostic framework to improve lesion classification through ensemble learning and dentin standardization.

Materials and methods: Five models—Support Vector Classifier (SVC), Nu-SVC, K-Nearest Neighbors (KNN) and Decision Tree—were trained on 144 pre-treatment periapical radiographs (70 cysts and 74 granulomas). Dentin standardization normalized grayscale values to ensure feature consistency. A weighted soft voting strategy was employed to integrate predictions, with model weights derived from individual diagnostic performance. Model performance was evaluated via five-fold cross-validation. Statistical significance among models …

2-O-Methylmagnolol Suppresses Oral Squamous Cell Carcinoma Progression By Targeting C-Myc Signaling, Chi-Yuan Chen, Tzong-Ming Shieh, Chin-Chuan Chen, Yann-Lii Leu, Yu-De Chu, Miaw-Jene Liou, Yi-Tsen Liu, Hsin-Wei Lin, Kai-Yin Chen, Cheng-Chia Yu, Tong-Hong Wang

Journal of Dental Sciences

Background/purpose: Oral squamous cell carcinoma (OSCC) is characterized by aggressive growth and frequent recurrence, highlighting the need for novel targeted therapies. Magnolol, a bioactive compound derived from Magnolia officinalis, exhibits anti-tumor activity. In this study, we synthesized a methoxylated derivative, 2-O-methylmagnolol (MM1), and evaluated the anti-OSCC efficacy and underlying mechanisms of magnolol and MM1.

Materials and methods: Human OSCC cell lines (SAS and OECM1) were treated with magnolol or MM1. Cell proliferation, migration, and invasion were analyzed using functional assays. Cell-cycle distribution and apoptosis were assessed by flow cytometry and DiOC₆/PI staining. Protein expression of c-MYC and downstream regulators …

C-X-C Motif Chemokine Ligand 13 And The C-X-C Motif Chemokine Receptor 5–C-Jun N-Terminal Kinase–Nuclear Factor Kappa B–Matrix Metalloproteinase 9 Axis In Oral Squamous Cell Carcinoma Metastasis, Ju-Fang Liu, Kuan-Chou Lin, Po-Chih Hsu, Tsung-Ming Chang, Peng Chen, Ying-Sui Sun

Journal of Dental Sciences

Background/purpose: Metastasis is the leading cause of treatment failure in oral squamous cell carcinoma (OSCC). This study investigated the role of C-X-C motif chemokine ligand 13 (CXCL13) in OSCC metastasis and the underlying signaling mechanism.

Materials and methods: Integrative transcriptomic analysis was performed using three independent OSCC datasets to identify metastasis-associated genes. CXCL13 expression was examined in OSCC tissues and highly migratory sublines. The effects of CXCL13 on cell migration were evaluated in vitro. Pathway analysis, pharmacological inhibitors, and receptor silencing were used to investigate the downstream signaling pathway. An orthotopic tongue xenograft model was used to evaluate the effect …

Synergistic Effects Of Biodegradable Magnesium Alloy And Erythromycin On Osteogenic Activity, Kridtapat Sirisereephap, Kitipong Pasanaphong, Thanaphum Osathanon, Patcharapit Promoppatum, Tomoki Maekawa, Viritpon Srimaneepong, Meircurius Dwi Condro Surboyo, Kamolporn Wattanasirmkit

Journal of Dental Sciences

Background/purpose: Biodegradable magnesium (Mg)–based alloys are emerging as promising alternatives to non-resorbable titanium for bone regeneration because of their favorable mechanical properties and biodegradability. However, the interaction between Mg alloys and immunomodulatory agents in promoting osteogenesis remains poorly understood. This study aimed to evaluate the osteogenic effects of an Mg alloy on pre-osteoblasts and to investigate whether erythromycin enhances Mg-induced osteogenic differentiation through regulation of developmental endothelial locus-1 (DEL-1).

Materials and methods: WE43 Mg alloy disks were immersed in culture medium to produce Mg alloy-conditioned medium at concentrations of 25%, 50%, 75%, and 100%. MC3T3-E1 cells were treated with conditioned …

Modulation Of Osteoclast Differentiation By Epigallocatechin-3-Gallate (Egcg)/Type I Collagen–Coated Titanium Implant Surface In Vitro, Her-Hsiung Huang, Yu-Ying Cheng, Chia-Fei Liu, Wei-Cheng Chen

Journal of Dental Sciences

Background/purpose: Implant osseointegration depends not only on osteogenesis but also on coordinated bone remodeling. Our previous study demonstrated that epigallocatechin-3-gallate (EGCG)/type I collagen–coated titanium (Ti) surfaces enhance angiogenesis and osteogenic differentiation in vitro. However, their influence on osteoclast differentiation remains unclear. This study evaluated the modulatory effects of this coating on osteoclast responses in vitro.

Materials and methods: Sandblasted and acid-etched (SLA) Ti surfaces followed by alkaline treatment (SLAA) were prepared. Type I collagen was immobilized onto SLAA surfaces using the natural crosslinker EGCG (10 or 50 μg/mL). Surface morphology, roughness, hydrophilicity, and functional groups were characterized. RAW 264.7 macrophages …

Prevalences Of Vitamin B12 Deficiency, Pernicious Anemia, Macrocytosis, And Hyperhomocysteinemia In 532 Serum Gastric Parietal Cell Antibody-Positive Oral Mucosal Disease Patients, Yu-Hsueh Wu, Yi-Pang Lee, Julia Yu-Fong Chang, Yi-Ping Wang, Andy Sun, Chun-Pin Chiang

Journal of Dental Sciences

Background/purpose: Our previous studies found the serum gastric parietal cell antibody (GPCA) positivity in 12.3% to 26.7% of atrophic glossitis (AG), burning mouth syndrome (BMS), and oral lichen planus (OLP) patients. This study assessed whether GPCA-positive oral mucosal disease (GPCA⁺OMD) patients (including 284 AG, 109 BMS, and 139 OLP patients) had significantly higher frequencies of microcytosis, macrocytosis, anemia, serum iron and vitamin B12 deficiencies, and hyperhomocysteinemia than healthy control subjects (HCSs) or GPCA-negative OMD (GPCAˉOMD) patients.

Materials and methods: The mean corpuscular volume, blood Hb, and serum iron, vitamin B12, homocysteine, and GPCA levels were measured and compared …

Impact Of Immediate Restoration On Bone Remodeling In The Esthetic Zone: A Randomized Clinical Trial, Hao-Dong Wang, Xin-Hai Lin, Yi-Wen Wu, Hong-Run Zhou, Tai Zhao Wei, Xi Chen, Yi-Qun Tang, Ya-Qin Zhu, Long Jiang

Journal of Dental Sciences

Background/purpose: Traumatic loss of anterior teeth creates esthetic challenges and often requires immediate rehabilitation. Although immediate implant placement and restoration (IIPR) shortens treatment time, strict primary stability limits its use. A native-crown restoration supported by fiber-reinforced composite (FRC) splints may broaden the indications of immediate restoration, but its effectiveness remains unclear. This randomized controlled trial aimed to evaluate the effects of the novel immediate restoration following IIP on peri-implant bone remodeling in anterior esthetic zone trauma during osseointegration.

Materials and methods: Twenty participants undergoing IIP in the anterior maxilla were randomized into two groups. In the experimental group, immediate restorations …

Poly-Ε-Caprolactone/Multi-Wall Carbon Nanotube Composites As Osteoinductive Scaffolds For Alveolar Bone Regeneration,, Chien-Yang Yeh, Firdiansyah Rasyid

Journal of Dental Sciences

No abstract provided.

Comparative Effects Of Plasma Activation And Monomer Infiltration On The Surface Characteristics And Wettability Of 3d-Printed Permanent Crown Resin,, Pyi Phyo Win, Szu-Yu Lai

Journal of Dental Sciences

No abstract provided.

Integrated Analysis Of Single-Cell And Bulk Rna-Sequence Data Reveal Plasma Dendritic Cell-Related Diagnostic Model For Sjögren's Syndrome Based On Machine Learning,, Jinhao Zhang, Chunye Zhang

Journal of Dental Sciences

No abstract provided.

In Vitro Characterization Of Tooth-Derived And Dentin-Derived Graft Particles: Comparative Analysis Of Particle Size, Mineral Composition, And Cytotoxicity, Vinh Quang Luu, Nhan Duc Le

Journal of Dental Sciences

No abstract provided.

Deciphering Novel Targets In Salivary Gland Pleomorphic Adenoma By Integrating Plasma Proteomics And Parotid Transcriptomics Analyses, Yuchen Fan, Suning Mao

Journal of Dental Sciences

No abstract provided.

Daisaikoto Restores Mitochondrial Function And Suppresses Inflammation Induced By Periodontal Bacteria-Derived Lipopolysaccharides, Suzuka Tozawa, Kazuo Tomita

Journal of Dental Sciences

No abstract provided.

Mesenchymal Stem Cells Derived From Gingiva Normoxic Or Hypoxic-Preconditioning Allogenous Transplantation Affect Orthodontic Tooth Movement In Vivo, Alexander Patera Nugraha, Guang Hong

Journal of Dental Sciences

No abstract provided.

Vanadium-Functionalized Methyl Methacrylate-Based Primer Enhances Self-Curing And Bonding Of Resin Cement To Polyetheretherketone Restorations, Takeru Kondo, Jun Sato

Journal of Dental Sciences

No abstract provided.

Comparative In Vivo Study Of 3d-Printed Composite Bioceramic Scaffolds Fabricated By Distinct Pre-Processing Methods, Yu-Ting Li, Ju-Hsuan Yang

Journal of Dental Sciences

No abstract provided.

Fluvastatin Enhances Healing Of Medication−Related Osteonecrosis Of The Jaw (Mronj) Via Macrophage Polarization In A Rat Mronj−Like Model, Moa Tazaki, Koma Sanda

Journal of Dental Sciences

No abstract provided.

The Utility Of 3d Nanostructured Polyglycolic Acid Fibers For Novel Bone Regenerative Medicine, Itsuki Hayashi, Sayaka Nakamura-Yamada

Journal of Dental Sciences

No abstract provided.

Dual Effects Of 1Α,25-Dihydroxyvitamin D3 On Inflammation And Osteogenic Differentiation In Lipopolysaccharide-Stimulated Dental Pulp Stem Cells, Seong Eun Song, Giyoung Yun

Journal of Dental Sciences

No abstract provided.

A Scientometric Study On Research Trends And Characteristics Of Diabetes-Associated Periodontal Disease, Man Yang, Wenxiao Shi, Yan Xiong, Mengjie Chen, Linjun Shi, Huiwen Chen

Journal of Dental Sciences

Background/purpose: Substantial evidence demonstrates that diabetes is associated with periodontal disease. The purpose of this study was to analyze the scientometric characteristics and research trends of diabetes-associated periodontal disease (DAPD).

Materials and methods: All the papers on DAPD were comprehensively retrieved from the Scopus database. The years of publication were divided into before 2019 and after 2019 in the analysis of research trends.

Results: There were 2466 papers on DAPD, with total citations of 73,553 and the h index of 120. The trend of clinical investigations has highlighted periodontal examination, bone development, micro-computed tomography, tooth brushing, cardiovascular …

Socket Regeneration Using A Composite Regenerative Matrix: A Simplified Clinical Workflow With A 5-Year Follow-Up, Chi-Shan Tseng, Ming-Ko Li, Cheng-Chia Yu

Journal of Dental Sciences

Alveolar ridge remodeling following tooth extraction remains a clinical challenge in implant dentistry, particularly in the esthetic zone where dimensional stability of hard and soft tissues is critical for subsequent implant placement. This short communication describes a simplified clinical workflow for socket regeneration using a composite regenerative matrix (CRM) composed of concentrated growth factor (CGF), autologous fibrinogen glue (AFG), and freeze-dried bone allograft (FDBA). The technique was applied following atraumatic extraction in a single maxillary esthetic-zone case. Clinical and radiographic observations were documented for up to 5 years. Healing was uneventful, and cone-beam computed tomography performed at 3 months demonstrated …

Negative Pulp Sensibility Responses Do Not Necessarily Indicate Pulp Necrosis In Teeth Associated With Cystic Or Cyst-Like Lesions, Le Lu, Xiang Zhang, Ya Shen, He Liu

Journal of Dental Sciences

No abstract provided.

The Story Of A Taiwanese Female Student Studying Dentistry In Japan During The Japanese Colonial Period: An Oral History By Dr. Yueh-E Wu, Feng-Chou Cheng, Mei-Huei Li, Chien-Chi Chu, Chun-Pin Chiang

Journal of Dental Sciences

No abstract provided.