Digital Commons Network^™

Beyond Neurons: Impact Of Cannabidiol On Glial Cells In Ischemic Stroke, Victória Linden De Rezende, Khiany Mathias, Cinara Ludvig Gonçalves, Rafael Mariano De Bitencourt, Tatiana Barichello, Fabricia Petronilho

Faculty, Staff and Student Publications

Ischemic stroke triggers a complex cascade of events involving inflammation, oxidative stress, and glial cell dysfunction, all of which contribute to neuronal damage and impaired recovery. Glial cells (e.g., astrocytes, microglia, and oligodendrocytes) play key roles in neuroinflammatory responses, making them attractive targets for therapeutic modulation. Cannabidiol, a non-psychoactive phytocannabinoid from Cannabis sativa, exhibits anti-inflammatory, antioxidant, and neuroprotective properties. Preclinical evidence indicates that cannabidiol attenuates glial reactivity, reduces pro-inflammatory signaling, mitigates oxidative stress, and preserves blood-brain and intestinal barrier integrity in stroke models. Moreover, cannabidiol modulates key molecular pathways (e.g., nuclear factor-κB, tumor necrosis factor, and calcium-related signaling), contributing to …

Differentiation In The Human Urothelia Is Defined By Distinct Alternative Polyadenylation, Ninh B Le, Surbhi Sona, Briana Santo, Yi Zhang, Rosie Ou, R Allen Schweickart, Veena Kochat, William I Padron, Kunal Rai, Shih-Han Lee, Joo Mi Yi, Oliver Wessely, Byron H Lee, Angela H Ting

Faculty, Staff and Student Publications

Distinct epithelial cell states arise during differentiation, but mechanisms generating transcriptomic diversity among them remain poorly defined. The human ureter urothelium contains basal progenitor, intermediate cells, and terminally differentiated umbrella cells. Prior single-cell RNA sequencing revealed similar global gene expression profiles across these states, raising the question of how distinct identities emerge. Here, we show that alternative cleavage and polyadenylation (APA) introduces a major layer of transcriptomic diversity during urothelial differentiation, largely independent of changes in mRNA levels. Analysis of 13,544 urothelial cells identified hundreds of differentiation-associated APA events. Single-cell imaging revealed spatially specific APA patterns, and reporter assays demonstrated …

Differentiation In The Human Urothelia Is Defined By Distinct Alternative Polyadenylation, Ninh B Le, Surbhi Sona, Briana Santo, Yi Zhang, Rosie Ou, R Allen Schweickart, Veena Kochat, William I Padron, Kunal Rai, Shih-Han Lee, Joo Mi Yi, Oliver Wessely, Byron H Lee, Angela H Ting

Faculty, Staff and Student Publications

Distinct epithelial cell states arise during differentiation, but mechanisms generating transcriptomic diversity among them remain poorly defined. The human ureter urothelium contains basal progenitor, intermediate cells, and terminally differentiated umbrella cells. Prior single-cell RNA sequencing revealed similar global gene expression profiles across these states, raising the question of how distinct identities emerge. Here, we show that alternative cleavage and polyadenylation (APA) introduces a major layer of transcriptomic diversity during urothelial differentiation, largely independent of changes in mRNA levels. Analysis of 13,544 urothelial cells identified hundreds of differentiation-associated APA events. Single-cell imaging revealed spatially specific APA patterns, and reporter assays demonstrated …

Conned By The Enemy: The Entomopathogenic Fungus Metarhizium Anisopliae Lures And Kills Drosophila Suzukii, Ibrahim M. Farid, Shepard Ndlela, Paul G. Becher, Christopher W. Weldon, Abdelmutalab Ga Azrag, Helgi B. Schiöth, Samira A. Mohamed, Sunday Ekesi

All Peer-Reviewed Publications

BACKGROUND: Drosophila suzukii, commonly known as spotted wing drosophila (SWD), is a highly invasive and economically major pest that inflicts significant damage on soft-skinned fruit crops, including raspberries, blueberries, strawberries, blackberries, cherries and grapes. The recent invasion of D. suzukii in Africa represents a key impediment to the berry industry on the continent. Conventional control strategies for this pest rely heavily on chemical insecticides, which pose several adverse side effects on biodiversity and environmental health. In this study, we evaluated five Metarhizium anisopliae isolates (ICIPE 7, ICIPE 18, ICIPE 20, ICIPE 30, ICIPE 78) for development as a biopesticide for …

Mechanistic Insights Into The Antimicrobial Effect Of Benzodioxane-Benzamides Against Escherichia Coli, Lorenzo Suigo, Alessia Lanzini, Valentina Straniero, William Margolin

Faculty, Staff and Student Publications

Background/Objectives: The bacterial cell division machinery is emerging as an attractive target for antimicrobial compounds. FtsZ, a highly conserved essential division protein, is the target for a number of small molecules such as benzamides. Recent studies show that benzodioxane-benzamides (BDOBs) are among the most potent inhibitors of FtsZ function in Gram-positive bacteria, although their ability to inhibit Gram-negative FtsZ, in particular Escherichia coli FtsZ, has been more controversial.

Methods: Here, we use genetic and cytological methods to demonstrate that FtsZ of efflux pump-disabled E. coli can be efficiently targeted by BDOBs.

Results: We show that engineered mutants …

Payload Diversification Overcomes Resistance And Guides Sequential Antibody-Drug Conjugate Therapy In Breast Cancer, Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Fujii Takeo, Chalothorn Wannaphut, Jennifer A Maynard, Kyoji Tsuchikama, George W Sledge, Naoto T Ueno, Jangsoon Lee

Faculty, Staff and Student Publications

Purpose: Antibody-drug conjugates (ADCs) have transformed the treatment of breast cancer. FDA-approved ADCs such as trastuzumab deruxtecan (T-DXd), sacituzumab govitecan (SG), and datopotamab deruxtecan (Dato-DXd) have demonstrated clinical benefit; however, drug resistance will almost inevitably emerge in the metastatic setting, and there is no established strategy for selecting subsequent ADCs after disease progression to the first exposure to the ADC. This study aimed to define mechanisms of acquired resistance and evaluate rational sequencing approaches.

Experimental design: We generated breast cancer cell lines resistant to T-DXd (TDXd-R) and SG (SG-R). We assessed antigen expression, internalization, and resistance pathways, including efflux transporter …

Common Octopus (Octopus Vulgaris) Blooms Off The Southwest Of The Uk: History, Trends, Causes And Consequences.: Report On Work Package 1: History, Causes And Consequences Of Octopus Blooms, Bryce Stewart, Simon Thomas, Tim J. Smyth, Molly K. James, Emma Sullivan, Alice Hall, Tully Osmond, Emma Sheehan

School of Biological and Marine Sciences

Common octopus (Octopus vulgaris) are normally uncommon in UK waters, with a mainly south-westerly distribution linked to the species’ preference for warmer conditions. However, a major population bloom occurred off southwest UK in 2025, comparable to rare historical blooms recorded in 1899–1900, 1932–33 and 1950–51. The species’ rapid growth, high fecundity and short lifespan make its populations highly responsive to environmental conditions. Evidence from fisheries landings, scientific surveys and citizen science confirmed the bloom, with commercial fisheries landings of common octopus in 2025 being almost 65 times higher than recent annual averages. The 2025 bloom and all previous blooms were …

Expression Spectrum Of Te-Driven Transcripts In Human Adult Tissues, Benpeng Miao, Xinlong Luo, Amina Ademovic, Yushan Yang, Tao P Wu, Bo A Zhang

Faculty, Staff and Students Publications

Background

Transposable elements (TEs) are vital components of eukaryotic genomes and have played a critical role in genome evolution. Although most TEs are silenced in the mammalian genome, increasing evidence suggests that certain TEs are actively involved in gene regulation during early developmental stages. However, the extent to which human TEs drive gene transcription in adult tissues remains largely unexplored.

Results

In this study, we systematically analyze 17,329 human transcriptomes to investigate how TEs influence gene transcription across 47 adult tissues. Our findings reveal that TE-driven transcripts are broadly expressed in human tissues, contributing to both housekeeping functions and tissue-specific …

A Bovine Cell Line Resistant To Japanese Encephalitis Virus Entry But Permissive To Post-Entry Replication, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic orthoflavivirus that poses a significant global health threat. It causes severe neuroinflammatory disease in humans and reproductive failure in swine. Because of the broad host range and cell tropism of JEV, identifying animal cell lines resistant to infection has been a persistent challenge. In this study, we demonstrate that Madin–Darby bovine kidney (MDBK) cells are resistant to JEV infection yet remain fully permissive to viral replication when transfected with viral genomic RNA. Using immunoblotting, immunofluorescence, and flow cytometry, we show that MDBK cells, unlike the highly susceptible baby hamster kidney (BHK-21) cells …

Cxcr4-Targeted Theranostics In Non-Hodgkin Lymphoma: Present Evidence And Future Directions, Marcus Yoakam, Uma A. Obalapuram, Kameron Hahn, Samir Dalia

Student Publications

Non-Hodgkin lymphoma (NHL) is a biologically heterogeneous group of lymphoid malignancies with variable clinical outcomes despite advances in chemotherapy, immunotherapy, and cellular therapies. The C–X–C chemokine receptor 4 (CXCR4)/ C–X–C motif chemokine ligand 12 (CXCL12) axis plays a central role in lymphocyte trafficking, survival, and tumor microenvironment interactions. It is frequently dysregulated in NHL, making it an attractive target for precision oncology. CXCR4- directed theranostics combines molecular imaging and targeted radionuclide therapy, enabling real-time assessment of receptor expression, patient stratification, and personalized treatment delivery. Diagnostic positron emission tomography imaging using ⁶⁸Ga-pentixafor reliably visualizes CXCR4-expressing disease, while therapeutic agents, such …

Expression Spectrum Of Te-Driven Transcripts In Human Adult Tissues, Benpeng Miao, Xinlong Luo, Amina Ademovic, Yushan Yang, Tao P Wu, Bo A Zhang

2020-Current year OA Pubs

BACKGROUND: Transposable elements (TEs) are vital components of eukaryotic genomes and have played a critical role in genome evolution. Although most TEs are silenced in the mammalian genome, increasing evidence suggests that certain TEs are actively involved in gene regulation during early developmental stages. However, the extent to which human TEs drive gene transcription in adult tissues remains largely unexplored. RESULTS: In this study, we systematically analyze 17,329 human transcriptomes to investigate how TEs influence gene transcription across 47 adult tissues. Our findings reveal that TE-driven transcripts are broadly expressed in human tissues, contributing to both housekeeping functions and tissue-specific …

Beyond The Rash: Cutaneous Peripheral T-Cell Lymphoma As A Diagnostic Pitfall In Primary Care, Jared Hensley, Aura Calderon, Blake Martin, Cristina Kochi, Andres Suarez Parraga

School of Medicine Publications

Cutaneous manifestations of T-cell lymphomas may resemble common dermatologic or infectious conditions, increasing the risk of delayed recognition. We report a 78-year-old woman with known peripheral T-cell lymphoma (PTCL) who presented with a rapidly progressive, diffuse erythematous rash accompanied by cervical lymphadenopathy. Initial evaluation prioritized more common etiologies; however, inpatient medicine broadened the differential diagnosis, prompting early hematology and oncology consultation. Contrast-enhanced CT of the neck demonstrated multiple enlarged, partially necrotic left cervical lymph nodes consistent with disease progression. After multidisciplinary discussions and consideration of prognosis, the patient elected palliative-focused management with oncology follow-up. This case underscores the importance of …

Educational Design Models In Transition: A Comparative Review, Tofan Stofiana, Yeti Mulyati, Andoyo Sastromihardjo, Dadang Sunendar

Journal of Educational Research and Practice

A persistent gap between educational theory and classroom practice underscores the need for methodological approaches that are reflective, participatory, and context responsive. This study provides a comparative review of four major educational design models, including research and development (R&D); Analysis, Design, Development, Implementation, and Evaluation (ADDIE); design-based research (DBR); and educational design research (EDR), to examine their theoretical orientations and contemporary applications. A systematic literature synthesis and bibliometric mapping were conducted on 80 peer-reviewed journal articles indexed in Scopus between 2020 and 2025. The findings reveal that ADDIE remains the most frequently applied framework due to its procedural clarity and …

Mechanical Performance Evaluation Of Natural Composites Recycled Hemp Rice Bag Reinforced With And Without Silicon Carbide Particles, Gaber A. Elawadi, A. S. Fayed

Mansoura Engineering Journal

Significant progress has been made in the development of natural fiber-reinforced composite materials, owing to their potential to enhance mechanical properties while offering a greener and more sustainable alternative to traditional materials. These materials combine superior ecological benefits with enhanced mechanical performance. This study examines how recycled hemp fiber bags, including recycled hemp fibers obtained from rice hemp fiber bags, affect the mechanical characteristics of unreinforced and reinforced composites. Four composite systems were fabricated and tested: (1) HFR (randomly chipped recycled hemp fiber bags–epoxy composite), (2) HFB (continuous recycled hemp fiber bag–epoxy composite), (3) HFR SiC (randomly chipped recycled hemp …

A Constructive-Engaging Analysis Of Descarrtes (European) And Oyeshile (African) On Personhood, Olanrewaju Shitta-Bey

Comparative Philosophy

An examination of the nature of the human person provides a crucial foundation for understanding human life and identity. Conceptions of personhood have far-reaching implications beyond philosophy, influencing politics, ethics, religion, and epistemology. Beliefs about what constitutes a person shapes theories of rights, responsibility, and justice; moral views on duty and accountability; religious understandings of the soul and the afterlife; and epistemological reflections on the sources and limits of knowledge. Inquiry into human nature is therefore not merely abstract, but central to understanding social organization and individual conduct. This paper examines two influential accounts of human nature drawn from distinct …

The Coherence Of Navayāna Pragmatism: Ambedkar And The Pragmatist Reconstruction Of Buddhist Philosophy, Danish Hamid

Comparative Philosophy

This paper examines the philosophical coherence of B.R. Ambedkar’s reconstruction of his (Navayāna) Buddhism in relation to John Dewey’s pragmatism. Building on Scott Stroud’s reading of Ambedkar as a Deweyan Pragmatist (2017, 2023), it argues that Dewey’s evolutionary naturalism, his conception of growth, and pragmatic redefinition of concepts provide Ambedkar with the methodological tools for a naturalistic and socially reconstructive reinterpretation of Buddhist doctrines such as anicca (impermanence), dhamma (Buddha’s teaching), nirvāṇa (enlightenment), karuna (compassion), etc. Ambedkar transforms impermanence from a metaphysics of flux into an ethics of progressive growth, and recasts dhamma as a social morality grounded in fraternity …

Procedure To Evaluate Artificial Intelligence (Ai) Output For Educational And Clinical Tasks, Lynn Gilbertson, Cody Marie Busch, Jon Spike, Kaitlin Demoss, Jae Thompson

Teaching and Learning in Communication Sciences & Disorders

As generative artificial intelligence (AI) becomes more ubiquitous and accessible, there is a call to promote awareness, implementation, and evaluation skills among AI users. Free AI platforms offer unprecedented opportunities to transform how education and healthcare tasks are approached. However, a process for evaluating AI output has yet to be standardized. The current study implemented a structured process for guiding graduate and undergraduate students through critical evaluations of various AI platforms, promoting students' critical thinking and career readiness.

Review Of "The Age Of The Gas Mask: How British Civilians Faced The Terrors Of Total War" By Susan R. Grayzel, Kyle Pritchard

Canadian Military History

Review of The Age of the Gas Mask: How British Civilians Faced the Terrors of Total War by Susan R. Grayzel

Consanguinity, Jill Carpenter

Creative Nonfiction Magazine

No abstract provided.

The Modern-World System: Europe Or Asia? Fernand Braudel & André Gunder Frank, Charles Lemert

Fast Capitalism

Author Biography

Charles Lemert, Wesleyan University

Charles Lemert is an Emeritus Professor of Sociology at Wesleyan University and will soon publish: Americans Thinking America; The Bottom Turtle: Silence and Society; and. with Immanuel Wallerstein: Living Progressively in a Transitional Time.

Hamlet's Father: Hauntology And The Roots Of The Modern Self, Lukas Szrot

Fast Capitalism

In his 1949 work Hamlet's Ghost, Richard Flatter wrote of the ghost of Hamlet's father that the play ultimately belongs to him, to the ghost.   Modernity, like the play, belongs to its ghosts, to its dead fathers haunting their wayward sons, the metaphysical specters it imperfectly endeavors to exorcise. Critical theory has often focused on the possibility, and the contours, of a utopia populated by liberatory spirit and liberated persons. Less well explored, however, are the implications for tradition, religion, and the transmission of culture at a metaphorical echelon—those ostensibly "pre-modern" ideas which the broader project of Enlightenment liberalism never …

The Master's Race: Phallic Whiteness In "The Young Savages", Graham Cassano

Fast Capitalism

This essay examines two parallel, historically contemporaneous, depictions of the Freudian master-slave dialectic. John Frankenheimer's first film, The Young Savages (1961), reconstructs Hank Bell's (Burt Lancaster) repressed transformation from Italian racial other into a white ethnic. In doing so, the film approaches the possibility that race itself may be a kind of social construction. To get at this notion, the film explores the meaning of race in an overtly psychoanalytic language. In fact, The Young Savages echoes the argument of Jacques Lacan's nearly contemporaneous essay, "The subversion of the subject and the dialectic of desire in the Freudian unconscious." Lacan's …

Have A Heart For The Holocene: The Politics Of Ark Activism, Collaborative Conservation, And Sponsored Survival At Museums, Timothy W. Luke

Fast Capitalism

No abstract provided.

The Biopolitical Conditions Of Sovereign Performativity, Benjamin Taylor

Fast Capitalism

No abstract provided.

The Tragedy Of The Commonplace: Clichés In The Age Of Copyright, Jakob Norberg

Fast Capitalism

No abstract provided.

Changing Bodies And The History Of Anthropometrics, Moramay López-Alonso

History of Medicine Seminar

No abstract provided.

Review Of "The Ones We Let Down: Toxic Leadership Culture And Gender Integration In The Canadian Forces" By Charlotte Duval-Lantoine, Rebecca Murray

Canadian Military History

Review of The Ones We Let Down: Toxic Leadership Culture and Gender Integration in the Canadian Forces by Charlotte Duval-Lantoine

Review Of "Frontier Science: Northern Canada, Military Research, And The Cold War, 1945-1970" By Matthew S. Wiseman, Adam Coombs

Canadian Military History

Review of Frontier Science: Northern Canada, Military Research, and the Cold War, 1945-1970 by Matthew S. Wiseman

Review Of "For Russia With Hitler: White Russian Émigrés And The German-Soviet War" By Oleg Beyda, Matthew Barrett

Canadian Military History

Review of For Russia with Hitler: White Russian émigrés and the German-Soviet War by Oleg Beyda

Photocrosslinkable Morin-Loaded Gelatin-G-Gma Composite Hydrogel For Accelerating Burn Wound Healing: In Vitro And In Vivo Assessments, Samar A. Salim

Nanotechnology Research Centre

The impaired skin regeneration, scarring, and delayed healing make the management of burn injuries a challenging task. We designed a photopolymerized hydrogel of gelatin-grafted with glycidyl methacrylate (GMA) for burn management applications. Hydrogel was incorporated with Morin, a plant flavonoid that was originally isolated from the Moraceae family, with known anti-antioxidant and antifibrotic activities. The physicochemical characterization of the resultant hydrogel, including its gelation time and swelling properties, was conducted. The characterization results indicated that the hydrogel development was successful, exhibiting well-established porosity, as evidenced by the SEM images. In vivo evaluation demonstrated improved tissue regeneration characterized by enhanced collagen …