Digital Commons Network^™

Physics-Based Multi-Hazard Fragility Modeling Of Low-Rise Wood-Frame Buildings Under Hurricane-Induced Wind And Surge, Mumtasirun Nahar, Abdullah M. Braik, Mohammad Syed, Maria Koliou, Petros Sideris

Civil & Environmental Engineering Faculty Publications

Coastal buildings are exposed to concurrent hurricane-induced wind and storm surge, yet their vulnerability is commonly assessed using separate single-hazard models that neglect hazard interaction. This study developed a physics-based finite element framework to evaluate the performance of a low-rise light wood-frame building under simultaneous wind and surge-wave loading. A detailed three-dimensional model was constructed using a sub-assembly strategy that explicitly represented framing members, sheathing systems, and critical connections. Structural response was evaluated across a hazard space defined by wind speed, still-water depth, and flow velocity, and component- and system-level fragility functions were derived using Monte Carlo simulation and generalized …

Quantification Of Illicit And Prescription Drug Residues In Pasteurized Donor Human Milk: A Comprehensive Analytical Approach, Michelle Brenner, Kaytlin Krutsch, Ashlynn Baker, Dhavalkumar Patel, Palika Datta

Department of Pediatrics Faculty Publications

Objective

To assess the risk of infant exposure to illicit and prescription drug residues, from infant-ready, pooled, pasteurized donor human milk (PDHM) provided by healthy, screened, voluntary non-remunerated donors to a not-for-profit milk bank.

Study Design

A total of 150 pooled samples, representing 742 donor contributions, were analyzed for 15 illicit and prescription drug analytes using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Detected concentrations were evaluated for clinical significance.

Result

All 150 PDHM samples tested negative for residues of marijuana, amphetamines, benzodiazepines, fentanyl, hydrocodone, and morphine. Three samples (2%) contained trace amounts of oxycodone (< 0.6 parts per billion), yielding exposures over 455 times lower than established standards for infant safety (Relative Infant Dose: < 0.022%; standard safety threshold 10%).

Conclusion

Comprehensive analysis detected no clinically …

Endothelin-1 In The Failing Fontan: Pathobiology, Precision Therapeutics, And Future Trial Design, Iman Maiza

Department of Pediatrics Faculty Publications

The Fontan circulation, devised as definitive palliation for single-ventricle congenital heart disease, imposes systemic venous hypertension, loss of pulmonary arterial pulsatility, and restricted preload reserve. These hemodynamic trade-offs progressively injure the pulmonary vasculature, liver, and lymphatic system, producing late morbidities including elevated pulmonary vascular resistance, Fontan-associated liver disease (FALD), protein-losing enteropathy, and arrhythmias. Endothelin-1 (ET-1), a potent vasoconstrictor and profibrotic mediator, plausibly unifies these complications. Mechanistic studies demonstrate ET-1 upregulation in failed Fontan lungs, activating PLC–Ca²⁺, RhoA/ROCK, and MAPK/ERK cascades to drive vasoconstriction and remodeling. In cirrhotic livers, ET-1 localizes to stellate cells, promoting contraction and fibrogenesis, mechanisms biologically relevant …

Vape Counseling At Pediatric Practices: The Rethink Vape Toolkit, Paul T. Harrell, Ann L. Edwards, Natasha K. Sriraman, Rebecca J. Slimak, Anisha S. Vanka, Amy C. Paulson, Kelli J. England

Department of Pediatrics Faculty Publications

Introduction:

E-cigarettes (Vapes) represent the most common form of tobacco used by adolescents, with one out of every 29 (3.5%) middle school students and one in every 13 (7.8%) of high school students vaping daily. This project aimed to increase vape screening and prevention education in youth visits at pediatric practices, to increase provider comfort with the topic of vaping, and to evaluate this educational process.

Methods:

A provider training and resource modules (talking points, billing codes, and shareable parent/teen-tailored resources) were developed. Providers (N = 32) completed an anonymous pre/postsurvey regarding their knowledge, comfort levels, barriers, and practices related …

Confessions In Cases Of Child Physical Abuse - A Capnet Study, Margaret R. Russell, Antoinette L. Laskey, Henry T. Puls, Suzanne P. Starling, Joanne N. Wood, Daniel M. Lindberg, Nancy S. Harper, Caitlin R. Mcnamara, Angela N. Bachim, Farah W. Brink, Lori D. Frasier, James D. Anderst, Porcia Vaughn, Kristine A. Campbell

Department of Pediatrics Faculty Publications

Objective

Prior research on confessions of physical abuse (PA) has explored mechanisms of injury. Little is known about conditions supporting a confession of abuse or how confessions may influence case outcomes.

Methods

This cross-sectional study of suspected PA in children age < 10 years at 10 centers participating in CAPNET, a multicenter child PA research network, included children with in-person Child Abuse Pediatrics (CAP) consultations between Feb 2021-Dec 2022 and excluded children without injury. Our focus was a confession of inflicted injury known to the CAP during clinical involvement. We compared child and clinical characteristics, out-of-home (OOH) placements, and arrests between cases with and without confessions. Multivariable models using generalized estimating equations (GEE) produced adjusted predicted probabilities (APP) clustering by CAPNET site.

Results

Confessions were known to CAPs in 115/4297 (2.7%) cases, with significant site variability. In a multivariable GEE model, confessions were more likely in cases with injuries with high-specificity for abuse (APP 6.0% vs 1.5%, p< 0.001), near-fatality (APP 5.5% vs 2.3%, p< 0.001), and older children (APP 4.8% vs 2.5%, p = 0.025). OOH placements and arrests were more common in cases with confessions. CAP awareness of confessions, OOH placements, and arrests did not follow racial or ethnic patterns seen in the US child welfare system. Lower socioeconomic status was associated with OOH placements and arrests but not CAP awareness of confessions.

Conclusions

Our findings highlight differences in case characteristics and outcomes where a confession is known to the CAP and raise questions about agency …

Bortezomib And Vorinostat In Combination With Mitoxantrone, Dexamethasone, And Pegasparaginase During Induction And Reinduction For Infants With Acute Lymphoblastic Leukaemia: A Multicentre Single-Arm Phase 1/2 Study, Tanja A. Gruber, Sima Jeha, Rebecca J. Deyell, Victor Lewis, Bill H. Chang, Eric J. Lowe, Jamie Frediani, Catherine Vezina, Bruno Michon, Michael Richards, Erin H. Breese, Thai-Hoa Tran, Norman Lacayo, Christine Bolen, Sunil Desai, Jennifer L. Pauley, Minxuan Huang, Emily Ashcraft, Cheng Cheng, Kirk R. Schultz, Linda Stork, Krysta Schlis, Van T. Huynh, Nathan Gossai, Yoav H. Messinger, Henrique Bittencourt, Terzah M. Horton, Uma Athale, Duncan Stearns, Deborah Schiff, Paul S. Gaynon

Department of Pediatrics Faculty Publications

Background: Acute lymphoblastic leukaemia in infants remains a therapeutic challenge. In preclinical studies we previously identified bortezomib and vorinostat as active agents against KMT2A rearranged (KMT2Ar) leukaemia. The aim of this study was to determine the tolerability of incorporating bortezomib and vorinostat into an acute lymphoblastic leukaemia-chemotherapy backbone for newly diagnosed infants with acute lymphoblastic leukaemia both with and without KMT2Ar.

Methods: In this single-arm phase 1/2 study conducted at 18 hospitals in the USA and Canada, we enrolled patients aged 1 year or younger at the time of diagnosis with newly diagnosed acute lymphoblastic leukaemia or undifferentiated leukaemia (with …

Incorporating New Guidelines Into School-Based Asthma Management, Jessica Michala Jindra, Angela Duff Hogan

Department of Pediatrics Faculty Publications

Purpose of the Review

The aim of this review is to identify barriers and provide solutions to the implementation of inhaled corticosteroid-formoterol as rescue therapy within schools.

Recent findings

GINA guidelines in 2019 and NAEPP in 2020 recommended inhaled corticosteroids combined with formoterol for a rescue inhaler over the traditional short-acting beta-agonist.

Summary

The findings discussed in this article describe the barriers to the implementation of inhaled corticosteroids combined with formoterol for a rescue inhaler in school systems. Traditional asthma action plans, school nursing and parental education, and differences in dosing are some barriers identified. This paper provides solutions for …

35 Individuals With Huwe1-Related Neurodevelopmental Disorder And Suggested Clinical Evaluations, Mindy H. Li, Deziree L. Coleman, Kelsey Hogan, Danielle Luz, Lindsay Bhandari, Newell Belnap, Tiffany Busa, Charles Coutton, Klaus Dieterich, Svetlana Gorokhova, Clara Hildebrandt, Rachel Logan, Milena Mariani, Manuela Morleo, Vincenzo Nigro, John Pappas, Rachel Rabin, Kelly Schoch, Angelo Selicorni, Vandana Shashi, Rebecca Spillman, Jennifer Sullivan, Charlotte Tardy, Samantha A. Schrier Vergano, Brock Grill, Kristin Baranano

Department of Pediatrics Faculty Publications

HUWE1 (HECT, UBA, and WWE Domain Containing E3 Ubiquitin Protein Ligase1, OMIM 300697), located at Xp11.22, encodes a ubiquitin ligase that is highly conserved across species. Genetic variants in HUWE1 described in multiple independent studies cause X-linked intellectual disability, including in the patients identified by Juberg, Marsidi, and Brooks. This report describes 35 additional cases of individuals with variants in HUWE1 and suggested guidelines for clinical management. Our study includes several female cases, which have not been widely reported previously. Our findings confirm earlier reported clinical features including developmental delay, autism, hypotonia, short stature, and dysmorphic facial features as well …

Grant-O-Thon: A Designathon-Informed Grant Writing Program To Enhance Research Grant Writing Skills In Low- And Middle-Income Countries, Yusha Tao, Gifty Marley, Lauren Fidelak, Bin Yu, Yusuf Babatunde, Anita N. Walker, Anthony A. Attama, Andrea L. Conroy, Esther K. Afolabi, Dimitrios N. Vatakis, Iheanyi Omezuruike Okonko, Shyngle K. Balogun, Hongyun Fu, Xin Peng, Ijeoma M. Ifeorah, Patience Kukundakwe, Alexandra Cossio, Ifeoma M. Obionu, Ángela Patricia Beltrán López, Matthew Idowu Olatubi, Ucheoma C. Nwaozuru, Yuanyi Chen, Huachun Zou, Eneyi E. Kpokiri, Daniil Wu, Oliver Ezechi, Juliet Iwelunmor, Meredith Labarda, Jason J. Ong, Weiming Tang, Joseph D. Tucker

Department of Pediatrics Faculty Publications

Problem

Early career researchers in low- and middle-income countries (LMICs) face substantial barriers when writing research grants and have limited writing support. A designathon approach was applied to organize a grant writing workshop (grant-o-thon) tailored for early-career LMIC researchers. This study describes the planning, implementation, and participant-reported outcomes of the grant-o-thon as a participatory training approach.

Approach

Grant-o-thons were organized in 2023, 2024, and 2025. Consistent with the designathon model, the grant-o-thon was structured in three stages: preparation using co-creation with end-users, intensive collaborative teamwork supported by mentor feedback and weekly writing deliverables, and structured follow-up. Each grant-o-thon consisted of …

Gender Differences In The Association Between Adverse Childhood Experiences And Drug Use: Findings From A Community-Based Survey In China, Hongyun Fu, Elizabeth Monk-Turner, Xiushi Yang

Department of Pediatrics Faculty Publications

Background

While adverse childhood experiences (ACEs) are widely recognized risk factors for behavioral health problems, including drug use, prior research has largely been conducted in Western countries, focused primarily on males, and relied on convenience samples without comparison groups of nonusers. Limited work has examined the impact of ACEs on drug use in non-Western contexts. This study examines gender differences in the relationship between ACEs and drug use in China, using data from a population-based probability sample survey.

Methods:

Cross-sectional data were collected in 2019 from one city in Yunnan Province in Southwest China and one city in Guangdong Province …

Empowering Caregivers, Protecting Patients: An Ambulatory Central Line Quality Improvement Initiative For Education And Follow-Up, Elizabeth Martinez, Belinda Bordeaux, Stephanie Langevin, Cristina Herrera, Mary Smith, Merissa Doyle, Barbara Stein, Kimberly Poshar, Marissa Dunlap, Allison Silva, Eric Werner

Department of Pediatrics Faculty Publications

Introduction: Preventing ambulatory central line-associated bloodstream infections (A-CLABSIs) is challenging due to inconsistent education, follow-up, and home care practices. Pediatric intestinal failure (IF) patients are particularly vulnerable. This institution aimed to decrease its IF A-CLABSI rate by 25% within 2 years through a standardized, step-by-step central line care education program.

Methods: Using the Toyota problem-solving method, the team reviewed data and performed a root cause analysis of the prioritized problem, implementing a 2-phase education program. Phase 1 delivered in-hospital education using videos, handouts, and hands-on teaching with skill demonstration. The existing pediatric central line skills class was expanded and offered …

Type 2-Low Asthma: Epidemiology, Multiomics, And Emerging Therapies, Philip Mendez, Alanis Rosado, Juan Carlos Cardet

Department of Pediatrics Faculty Publications

Purpose of Review

Type 2-low (T2-low) asthma, characterized by low blood and sputum eosinophils and low fractional exhaled nitric oxide (FeNO), remains a major unmet need because of frequent corticosteroid insensitivity and a lack of targeted therapies. This review summarizes recent advances in its epidemiology, multiomics, and therapeutic landscape.

Recent findings

T2-low inflammation is highly prevalent, affecting approximately 14%-39% of adults with current asthma and up to 60% of children. Its pathogenesis reflects complex non-T2 mechanisms, including IL-17 signaling, innate immune activation through IL-1β and IL-33, and neutrophilic inflammation. Heterogeneity is further shaped by systemic factors such as obesity and …

Red-Sensitive Photopolymerizable Glass For Fabrication Of Holographic Optical Elements, Jamshed Aftab, Izabela Naydenova, Tatsiana Mikulchyk

Research Outputs: 2025-Present

Water-resistant photopolymerizable glass, which represents a photopolymerizable hybrid sol–gel (PHSG) material, has emerged as a compelling platform for holographic optical elements fabrication (HOEs), owing to its exceptional mechanical and thermal robustness with high holographic recording performance. Until now, PHSG compositions have been limited to blue/green-sensitive systems, despite significant interest in extending sensitivity into the red spectral region. Here, we report the first holographic photopolymerizable glass with sensitivity to red light, achieved through the incorporation of methylene blue (MB) as a photosensitizer and N-phenylglycine (NPG) as a co-initiator, with an optimal MB/NPG ratio of 1:150. This PHSG composition is capable of …

Accuracy Of Voluntary Force Modulation During The Isometric Mid-Thigh Pull, S. Alexander Long, Olivia Vadas, Stephanie Balint, Michael H. Stone, Christopher Taber

Exercise Science Faculty Publications

The purpose of this study was to investigate voluntary force modulation accuracy during the isometric mid-thigh pull (IMTP) and to investigate biological sex and relative strength as factors relating to error. Strength-trained males (n = 18) and females (n = 18) completed ascending (ASC) (25%, 50%, 75%) or descending (DESC) (75%, 50%, 25%) submaximal testing followed by maximal testing. Subjects rested before completing the opposite submaximal testing sequence. External feedback was not provided during testing. Measured and intended (INT) forces were analyzed with two-way repeated-measures ANOVAs with within- (ASC, DESC, and INT) and between-subject factors (male or female). Independent-samples t-tests …

A Dual-Domain Face Forgery And Deepfake Detection Framework, Neha Pradyumna Bora, Pradyumna Mulchand Bora, Rushikesh Sanjay Kumavat, Raunak Manoj Gangwal, Prit Sandesh Jain, Hardik Vijay Ostwal

Mansoura Engineering Journal

Concerns about media authenticity, privacy, and information security arising from rapid advances in deepfake technology have made it increasingly important to detect manipulated facial content reliably. The primary goal of our work was to develop a deepfake detection model with high generalization across multiple datasets. To achieve this, we developed a hybrid detection approach that combines spatial visual texture analysis and frequency-domain analysis to detect manipulated facial images. Our approach includes convolutional spatial feature extraction from facial images and frequency—domain representations of the same images obtained by applying the fast Fourier transform. To help alleviate concerns about overreliance on frequency—domain …

Multi-Method Energy Optimization: Linear Programming, Hmm Modeling And Arduino-Based Simulations, A.E. El-Alfy, Eman Elayat, M. A. E. Sheta

Mansoura Engineering Journal

This paper presents the simulation and optimization of hybrid energy systems integrating renewable energy and storage technologies. Linear Programming (LP) is utilized to minimize operational costs, while a Hidden Markov Model (HMM) improves reliability by accurately predicting energy flow. Hardware-in-the-loop simulation in Proteus demonstrates efficient energy trading, significantly reducing dependence on conventional sources. The results from a 24-hour analysis show that the system achieves an 87.14% load factor, indicating high efficiency in utilizing the system's rated capacity, 26.23 system reliability, and a 20% improvement in cost efficiency and enhanced sustainability, providing a robust and practical framework for hybrid energy solutions …

Dielectric And Thermal Ageing Characteristics Of Al₂O₃ Nanofluid-Impregnated Cellulose For Power Transformer Applications, Shaymaa A. Qenawy, Basma Eltlhawy, Hussein M. Waly

Mansoura Engineering Journal

As nanotechnology progresses, nanofluids have emerged as promising candidates for transformer insulation due to their enhanced dielectric and thermal properties. This study evaluates the degradation behavior of a mineral oil-cellulose system compared to an alumina (Al₂O₃) nanofluid-cellulose system. Accelerated thermal ageing tests were conducted at 120°C for a 20-day duration, simulating approximately 20 years of field service. Key findings reveal that the nanofluid-impregnated paper exhibited superior mechanical longevity, characterized by a 3% reduction in the tensile strength deterioration rate relative to mineral oil samples. Initially, the AC breakdown voltage (BDV) of the nanofluid was 10.85% higher …

Preserving The Identity Of Historic Coptic Orthodox Monasteries In Egypt Under Contemporary Pressures: Case Studies Of St. Bishoy And St. Anthony Monasteries, George Medhat Adeeb, Mohamed Khairy Amin, Mohamed Ahmed Al-Sherbiny

Mansoura Engineering Journal

Historic Coptic Orthodox monasteries in Egypt (HCOMEs) represent an important component of Christian heritage, integrating architectural value with enduring spiritual traditions that reflect both tangible and intangible dimensions. However, contemporary development and changing functional demands are generating transformations that may affect their identity if not carefully managed. Despite extensive scholarship, limited research has systematically examined these transformations through a structured evaluative approach. This study addresses this gap by developing and applying an analytical and evaluation framework to examine how contemporary pressures influence the historical, architectural-spatial, and spiritual identity of HCOMEs, and to assess the effectiveness of monastic responses. The framework …

Self-Lubricating Nano-Coatings For Tribological Applications: Materials, Mechanisms, And Ai-Driven Design Approaches, Viral Panara, Vikas Panchal, Satayu Travadi, Harmish Bhatt, Madhav Oza

Mansoura Engineering Journal

Self-lubricating coatings with nanostructured structures are becoming crucial in order to decrease friction and wear in critical tribological systems like precision tools, biomedical implants, aerospace actuators, and engines. In this review, the most representative nano-engineered solid lubricants such as transition metal dichalcogenides, diamond-like carbon, hexagonal boron nitride, graphene, MXenes and hybrid multilayer architectures are discussed. The focus of the discussion is on the most important tribological mechanisms like lamellar sliding, oxidative adaptation, tribofilm formation, transfer-film development, and nano-asperity contact evolution. It also summarizes the most significant deposition techniques, such as cathodic arc physical vapor deposition, chemical vapor deposition, plasma-based deposition, …

1-D And 2-D Hadamard Transformations For Protein Sequence Analysis, Esraa A. Ahmed, Abed M. Nasr, Marwa A. Abd Elwahaab

Mansoura Engineering Journal

Similarity/dissimilarity studies follow alignment-based or alignment-free methods. Many alignment-free methods involve selecting physicochemical properties. Most previous articles don’t explain their criteria for choosing the properties used. These heuristic approaches have, by definition, undeterministic and unreliable performance. Hence, there is a need for a powerful proxy for similarity/dissimilarity by a deterministic, reliable approach.

In this study, a physicochemical property is selected by a multi-criteria sequential selection process. The amino acids composing any protein sequence are replaced with their values of the chosen property to produce a discrete signal in the time domain.  The one-dimensional discrete Hadamard transforms our signal from the …

Quantifying Hemodynamics In Implantable Cardiac And Renal Support Devices Using Computational Modeling, Alexander D. De Oliveira

Summer Research Program Abstracts

No abstract provided.

The Desecration Of Sacred Spaces In The Dead Sea Scrolls And Pre-Nicene Latin Christianity: A History Of Interpretation Of The Eschatological Motif Of The Defiling Enemy In Apocalypticism, Rodrigo De Galiza Barbosa

Dissertations

Problem

The motif of the polluting enemy in the sanctuary is an important point of departure in the articulation of community boundaries in Hebraic-Christian culture. This particular motif has the potential to explain, at least in part, how Jewish and Christian communities would have understood the apocalyptic evil other and, from this understanding, set boundaries for their holy communities. Texts such as Dan 8 and 2 Thess 2 have been the locus classicus of the so-called antichrist, and ever since their interpretation has shaped how Christians and Jews have understood the enemy to their respective communities. In the history of …

Stringency Of Family Firms And Owner-Managers In The Transition To Low-Carbon Emissions, Morten Bennedsen, John Doukas, Halit Gonenc

Finance Faculty Publications

This study examines the relationship between carbon emissions and stock returns, with a focus on the role of family ownership and management. Using a sample of 435 publicly listed firms from 14 Western European countries over the period 2010–2020, we explore whether financial markets perceive family-controlled firms, particularly those led by family-member CEOs, differently in terms of their commitment to sustainable practices. Consistent with prior research, our results indicate that firms with higher emissions earn higher stock returns while simultaneously experiencing lower market valuations, consistent with the presence of a carbon risk premium driven by elevated carbon transition risk. Crucially, …

Does Bidder Complexity Affect Market Reactions To M&A Decisions?, Rajib Chowdhury, John A. Doukas

Finance Faculty Publications

We examine whether and how bidder complexity influences investor reactions to merger and acquisition (M&A) announcements. Using an established measure of complexity, we find a significant positive relationship between acquiring firm complexity and cumulative abnormal returns (CAR). This suggests that investors perceive more complex firms as capable and value-enhancing participants in M&A activities. The association is particularly strong for bidders with high operating risk, greater R&D intensity, and larger firm size. We also find that complex bidders tend to offer higher takeover premiums. Overall, our study contributes to the literature by demonstrating that bidder complexity is an important determinant of …

Tail Dependence: The Impact Of Risk Spillovers On Real Estate Markets In Times Of Economic And Geo-Political Uncertainty, Stanimira Milcheva, Simon Stevenson, Lingshan Xie

Finance Faculty Publications

The first half of the 2020's has seen a degree of economic and geo-political uncertainty not observed since the 1970s. This paper looks at how listed real estate is exposed to capital market shocks by estimating Conditional Value-at-Risk (CoVaR), which captures the sensitivity of real estate returns to extreme movements in broader equity markets. We use threshold Generalized Autoregressive Conditional Heteroskedasticity (tGARCH) and Copula co-dependency functions to accurately account for tail behavior and dependency structures. We find a significant increase in downside spillovers from capital markets to listed real estate during the Covid period across most countries. However, the response …

Patent Law's Trade Remedy, Michael Doane

Akron Law Faculty Publications

Although a trade remedy, not an intellectual property rights statute, Section 337 of the Tariff Act of 1930 plays an important and controversial role in the enforcement of intellectual property rights in the United States. As a trade remedy, Section 337 is designed and intended to promote innovation and investment in the United States through support of statutorily defined domestic industries against unfair competition from infringing imported products. This Article analyzes Section 337 as a trade remedy from the perspective of intellectual property rights owners and innovators such as start-up companies, universities, individual inventors, and other research and development-focused entities. …

Neurotensin Drives Theca Cell Migration During Ovulation In Primates, Jessica S. Miller, Megan A. G. Sage, Thomas E. Curry Jr., Diane M. Duffy

Department of Biomedical and Translational Sciences Faculty Publications

Theca cells are a critical steroidogenic cell type of the ovarian follicle and corpus luteum. The ovulatory luteinizing hormone (LH) surge (or human chorionic gonadotropin (hCG)) stimulates theca cell relocation from the stroma surrounding the dominant follicle to full integration into the developing corpus luteum. Luteinizing hormone/human chorionic gonadotropin also stimulates granulosa cells to produce local mediators of ovulation, including the peptide neurotensin (NTS). To determine if hCG-stimulated NTS regulates theca cell relocation within the ovulatory follicle, vehicle or an NTS receptor antagonist was injected into a macaque dominant follicle, and ovaries were removed 48 h after hCG administration. Additional …

P38 Mapk Is Involved In Epigenetic Regulation Of Fibrotic Genes In Replication Induced Senescence In Lung Fibroblasts, Shan Zhu, Jennifer Q. Zhou, Kan Wang, Ming-Lei Guo, Yan Sanders

Department of Biomedical and Translational Sciences Faculty Publications

Fibroblast activation is essential for tissue repair following injury; however, prolonged activation drives pathological fibrosis. Idiopathic pulmonary fibrosis (IPF), a progressive and age-associated lung disease, is characterized by aberrant fibroblast activation, with increasing evidence implicating senescent and near-senescent fibroblasts in its pathogenesis. However, the underlying mechanisms remain poorly defined. In this study, we investigated whether histone modification is involved in TGF-β1 treated lung fibroblasts and contributes to the fibrotic phenotype. Human IMR90 lung fibroblasts at low and high population doubling levels (LPDL and HPDL), as well as primary IPF fibroblasts, were used in this study. In response to TGF-β1, both …

Brief Pulses Of High-Level Fluid Shear Stress Enhance Metastatic Potential And Rapidly Alter The Metabolism Of Cancer Cells, Amanda N. Pope, Devon L. Moose, Guy O. Hudson, Hank R. Weresh, Marion R. Dykstra, Aabha Y. Joshi, Patrick Breheny, Eric B. Taylor, Michael D. Henry

Department of Biomedical and Translational Sciences Faculty Publications

Circulating tumor cells (CTCs) face challenges to their survival, including mechanical and oxidative stresses that are different from cancer cells in solid primary and metastatic tumors. The impact of adaptations to the fluid microenvironment of the circulation on the outcome of the metastatic cascade is not well understood. Here, we find that cancer cells exposed to brief pulses of high-level fluid shear stress (FSS) exhibit enhanced invasiveness and anchorage-independent proliferation in vitro and enhanced metastatic colonization/tumor formation in vivo. Cancer cells exposed to FSS rapidly alter their metabolism in a manner that promotes survival by providing energy for cytoskeletal remodeling …

Stat4-Dependent Regulation Of Neuroinflammation In Atherosclerosis, Natalie Stahr, Alina K. Moriarty, Shelby D. Ma, W. Coles Keeter, Woong-Ki Kim, Larry D. Sanford, Elena V. Galkina

Department of Biomedical and Translational Sciences Faculty Publications

Atherosclerosis is linked to an increased risk of cognitive decline, with chronic inflammation being a common feature of both pathologies. IL-12 activates STAT4 to regulate myeloid cell functions, and blockade of this pathway alleviates cognitive impairment in Alzheimer's models. However, the mechanisms connecting vascular pathology to neuroinflammation remain unclear. Here, we examine whether STAT4 functions as a common mediator of neuroinflammation in atherosclerosis. We demonstrate that LysM^Cre-specific STAT4 deficiency ameliorates deficits in long-term memory in low-density lipoprotein-deficient (Ldlr^-/-) mice fed a high-fat diet (HFD-C). STAT4 deficiency moderately reduces Ser199-phosphorylated Tau burden. Atherosclerosis alters brain immune …