Digital Commons Network^™

Surface Marker Identification To Capture Live Circulating Tumor Cells In Metastatic Triple-Negative Breast Cancer, Bree M Lege, Khushali J Patel, Brendan Panici, Ping Gong, Michael T Lewis, Matthew J Ellis, Chonghui Cheng

Faculty, Staff and Students Publications

Metastatic triple-negative breast cancer (TNBC) is highly aggressive and lacks targeted therapies. Circulating tumor cells (CTC) are invaluable for monitoring metastatic tumor progression and treatment response but are difficult to capture because of their rarity and heterogeneity. Surface-based staining for live CTCs is essential to preserve RNA quality in single cells, but current markers tend to perform poorly on more mesenchymal tumor cells such as TNBCs. To enhance live TNBC CTC detection, we developed a workflow for live CTC capture and single-cell RNA sequencing (scRNA-seq). Using a mouse model of metastatic TNBC, we identified four new CTC surface markers, AHNAK2, …

Concordance Of Fluorescence In Situ Hybridization, Whole-Exome Sequencing, And Chromosomal Microarray For Evaluating High-Risk Molecular Features In Meningiomas, Brenndan Crumley, Emilie Russler-Germain, Sonika Dahiya

2020-Current year OA Pubs

BACKGROUND: Meningiomas are the most common primary intracranial tumors, classified into three WHO grades based on histologic and/or molecular criteria. cIMPACT-NOW Update 8 underscores the increasing importance of molecular testing in grading and risk stratification, noting that morphologically WHO grade 1 tumors with concurrent 1p and 22q losses demonstrate clinical behavior comparable to WHO grade 2 meningiomas. This study aimed to assess the concordance of fluorescence in situ hybridization (FISH), chromosomal microarray analysis (CMA), and whole-exome sequencing (WES) in defining the molecular risk profile of meningiomas.

METHODS: A total of 113 meningiomas were analyzed by WHO grade to assess the …

01.01.06. Mute Swan, Cygnus Olor, Bill Pranty, Ann Hodgson, Florida Ornithological Society

01.01. <em>Anatidae</em> - Ducks, Geese, and Waterfowl

Of the seven species of swans that have been documented in Florida, only the non-breeding Tundra Swan (Cygnus columbianus) is native. The other six species, including the Mute Swan, have been imported into Florida and then released at various lakes across the state for aesthetic purposes. Mute Swans are native from Great Britain, Scandinavia, and Russia south through central Europe to the Balkans, Turkey, Kazakhstan, Siberia, and northern China.

Characteristics Of Cerebral Palsy In The Midwestern Us, Susie Kim, Kelsey Steffen, Lauren Gottschalk-Henneberry, Jennifer Miros, Amy Robichaux-Viehoever, Karen Taca, Bhooma R Aravamuthan

2020-Current year OA Pubs

OBJECTIVE: Cerebral palsy (CP) is the most common lifelong motor disability worldwide. Yet, data is limited on how CP manifests in the US. Our objective was to characterize and determine factors affecting functional outcomes in a large population of young people with CP in the Midwestern US.

METHODS: We integrated caregiver and clinician-facing standardized data entry into routine clinical care at a tertiary care CP center (https://bit.ly/CP-Intake-Methodology). We extracted this data for people with an ICD10 diagnosis of CP seen between March 22, 2023 and December 28, 2023 and used it to describe CP characteristics and determine factors affecting the …

A Single-Cell Atlas Characterizes Dysregulation Of The Bone Marrow Immune Microenvironment Associated With Outcomes In Multiple Myeloma, William C Pilcher, Lijun Yao, Yizhe Song, Kazuhito Sato, Julia T Wang, Reyka G Jayasinghe, Jennifer A Foltz, Mark A Fiala, Chiang I-Ling, Julie Fortier, Michael Slade, Stephen T Oh, Michael P Rettig, John F Dipersio, Shaji Kumar, Ravi Vij, Li Ding, Et Al.

2020-Current year OA Pubs

Multiple myeloma (MM) remains incurable despite advances in treatment options. Although tumor subtypes and specific DNA abnormalities are linked to worse prognosis, the impact of immune dysfunction on disease emergence and/or treatment sensitivity remains unclear. We developed an Immune Atlas of MM by generating profiles of 1,397,272 single cells from the bone marrow (BM) of 337 newly diagnosed participants and characterized immune and hematopoietic cell populations. Cytogenetic risk-based analysis revealed heterogeneous associations with T cells of BM, with 17p13 deletion showing distinct enrichment of a type 1 interferon signature. The disease progression-based analysis revealed the presence of a proinflammatory immune …

Drone Dichotomy: Investigating The Application Of Unmanned Aerial Systems For Offensive And Defensive Chemical, Biological, Radiological, And Nuclear-Related Tasks, Evan Jacob Martinovitch

Graduate Theses/Dissertations

This thesis investigates the application of unmanned aerial systems (UAS) for both offensive and defensive chemical, biological, radiological, and nuclear (CBRN) tasks to determine if they serve as a force multiplier on the modern battlefield. As CBRN weapons reemerge as realistic threats alongside the rapid proliferation of UAS, the intersection of these fields presents unique challenges and opportunities. Through a methodology that examines the history of the UAS-CBRN intersection, military doctrine, and the status of UAS technology, this research evaluates the capability of current and emerging UAS against specific CBRN delivery, reconnaissance and surveillance, and decontamination tasks. The analysis conducted …

Gene Editing And The Accessibility Of Bioterrorism: Rethinking Global Biosecurity In The Post-Covid-19 Era, Nicholas Morales

Graduate Theses/Dissertations

COVID‑19 demonstrated how a single biological event can strain health systems, disrupt supply chains, undermine national security, and erode public trust. This shock occurred as biotechnology became dramatically more accessible: gene editing and synthetic biology tools once limited to specialized laboratories are now widely available through inexpensive equipment, open‑source data, and decentralized expertise.

This expanded access accelerates innovation but also broadens the threat landscape. The ability to reconstruct pathogens using publicly available sequences and mail‑order DNA shows that complex biological synthesis is no longer confined to state programs. These risks are amplified by opaque biotechnology activities in China, Russia, and …

Using 1:1 Behavior-Analytic Instruction To Enhance Outcomes Of A Social-Emotional Learning Curriculum: A Retrospective Single-Case Clinical Evaluation, Cierra Vandermeer

Graduate Theses/Dissertations

Social-Emotional Learning (SEL) is the process through which people acquire and apply skills to manage emotions, form relationships, make responsible decisions, and pursue personal and shared goals, and these competencies shape identity development and daily functioning (Collaborative for Academic, Social, and Emotional Learning [CASEL], 2020). While there are many SEL programs and curricula that exist for school-aged children, there are few curricula that a) have a focus on 1:1 instruction for preschool to primary-aged children, and b) integrate and incorporate Applied Behavior Analysis (ABA) principles. This thesis reviewed a retrospective case study of a six-year-old female-identifying student who participated in …

When Speech Speaks Louder Than Truth: Language Variation And Credibility In The Courtroom, Melanie E. Spinnie

Graduate Theses/Dissertations

This study aims to investigate how linguistic variation affects perceptions of credibility in courtroom settings. It focuses on how jurors, judges, and other individuals in the legal system may exhibit bias (consciously or unconsciously) based on how a defendant or witness speaks-- this could include speech disorders as well as differences in dialect, accent, and speech style. Through review of relevant literature, review of a relevant historical case, conducting a survey, and conducting a semi-structured interview, this study explores whether certain speech patterns or characteristics are disproportionately associated with negative judgments during legal proceedings, such as reduced credibility and trustworthiness. …

Europe In The Indo-Pacific - Implications For The United States, Joshua G. Zinicola

Graduate Theses/Dissertations

Within the last five years the Indo-Pacific has become a region of increasing interest to European NATO members. In particular, the United Kingdom, France, and Italy have assigned strategic importance for their national security to the region, signing cooperative agreements with regional allies of the United States and deploying significant forces to the region. These agreements include commitments to jointly develop new technologies and combat systems for employment in both Europe and the Indo-Pacific, such as the Global Combat Air Programme (GCAP), and the Trilateral security partnership between Australia, the United Kingdom, and the United States (AUKUS). They also include …

Stopping The Buck: What Made The Truman Committee Uniquely Successful In Its Oversight Of World War Two Military Contracts, Victoria Kelley-Chiarelli

Graduate Theses/Dissertations

During World War Two, Harry S. Truman led the Senate Select Committee to Investigate the National Defense Program, also known as the Truman Committee, in its oversight of military contracts and the war effort. The Truman Committee saved the U.S. government billions by identifying and discouraging waste, fraud, and abuse, and saved many armed forces personnel’s lives through identifying defective products. Truman’s background in World War One service and his time as a local official in Missouri show signs of the integrity and commitment to good governance that made the Truman Committee so successful. The Truman Committee’s membership, staff, principles, …

Exploring Factors Mediating White-Nose Syndrome In Little Brown Bats, Carly R. Trujillo

Graduate Theses/Dissertations

The little brown bat (Myotis lucifugus) has experienced drastic population declines across North America due to the recent disease white-nose syndrome (WNS). Previous studies have identified putatively adaptive alleles (PAAs) that may give individuals a greater likelihood of survival. However, there could be geographic-specific adaptation, creating different adaptive landscapes. Additionally, there may be energetic trade-offs between disease survival and reproductive output, and it is not clear what the long-term survival outcomes for the species may be as a result. Long-term evolutionary outcomes are unclear because this species has a lifespan exceeding the arrival of WNS and is difficult …

Heterogeneous Robots Cooperation Via Multi-Agent Reinforcement Learning, Rehab Uddin Shawon

Graduate Theses/Dissertations

Enabling heterogeneous robots with diverse capabilities, roles, and task responsibilities to coordinate effectively in complex, dynamic environments remains a fundamental challenge in autonomous multi-robot systems. Multi-Agent Reinforcement Learning (MARL) provides a promising framework for decentralized cooperation. However, most existing MARL approaches assume homogeneous agents or fixed single-task settings and suffer significant performance degradation as the number of robot and task types increases. This thesis presents a scalable shared-policy MARL framework that allows heterogeneous robots to learn specialized behaviors for individual, sequential, and collaborative tasks involving temporal and spatial dependencies through a single neural policy. I first embed robot identity directly …

The Effect Of Sex On A-To-I Editing In Skeletal Muscle During Inflammation, Skyler Lee

Graduate Theses/Dissertations

Adenosine-to-inosine (A-to-I) RNA editing is a post-transcriptional mechanism mediated by ADAR enzymes that can alter RNA sequence, protein function, and gene regulation. Because ADAR1 is upregulated during inflammation and plays a critical role in innate immunity, previous studies investigated the effects of acute inflammation on RNA editing in male and female mice. Lipopolysaccharide (LPS) was used to induce inflammation, and RNA editing levels were examined in the heart, brain, and skeletal muscle. While editing levels in the heart and brain remained unchanged, skeletal muscle exhibited tissue- and sex-specific differences in editing. In this thesis, LPS treatment affected selective editing, increasing …

Student Perspectives Of A Rubric-Based Performance Assessment In A Secondary Large Ensemble Classroom: Fairness, Clarity And Learning, Jeffrey S. Payne

Graduate Theses/Dissertations

The purpose of this study was to investigate student perceptions of a rubric-based performance assessment within a secondary large ensemble music program.  Seventy high school students enrolled in a high school band class completed a rubric-based performance assessment that featured personalized feedback and opportunities for resubmission  Using a mixed-methods approach, 17 students in the class voluntarily participated in a survey, the results of which I used to study my own use of assessment.  The survey consisted of eight Likert-scale questions and four open-response questions which were used alongside data regarding the resubmission rate and teacher documentation of instruction. Results indicated …

Advancing Hate Speech Detection: Binary And Multiclass Approaches From Traditional Methods To Parameter-Efficient And Ontology-Guided Language Models, Mahmoud Abusaqer

Graduate Theses/Dissertations

The widespread proliferation of hate speech on social media platforms poses significant challenges for content moderation and user safety, requiring automated systems that are simultaneously accurate, efficient, and capable of fine-grained distinctions. This thesis investigates hate speech detection through five published manuscripts organized into two complementary threads: binary detection (hateful vs. non-hateful) and multiclass detection across demographic targeting categories. The binary thread progresses from a broad 38-model baseline spanning traditional machine learning, deep learning, and transformer architectures (where RoBERTa reaches 91.48% accuracy and CatBoost remains competitive at 88.60%) to parameter-efficient adaptation, in which Low-Rank Adaptation (LoRA) of large language models …

Knowledge And Attitudes Of Medical Students About Research Methodology, Statistics, And Statistical Inference: An Assessment For Clinical Research Comprehension Skills, Laith Ashour, Lama Al-Mehaisen, Afnan Rababa, Osama Alrjoob, Moutaz Amayrh, Malak Al-Rabe'e, Qusai Al-Anasweh, Hala Al-Miqdadi, Nada Freihat, Mohammad Al Khreisha, Ala' Al Doaikat, Al-Mo'tasem Bellah Al-Rahamneh, Hamzeh Hatamleh, Fayez Zedat

Health Professions Education

Objectives: Medical students require a solid understanding of statistics in research papers. However, literature lacks sufficient studies on their proficiency, especially on Jordanian aspect. Methods: We performed a cross-sectional study on 361 medical students to evaluate their attitudes toward statistics, their knowledge in research methodology, and their ability to correctly interpret commonly used statistics in medical research. Statistical attitudes were assessed using three scales (value, difficulty, and interest) from the SATS-36 scale. Additionally, a novel author's-validated scale was created to measure students' research methodology knowledge. The scale validity was assessed using Confirmatory Factor Analysis (CFA). Multiple linear regression analysis was …

Beyond Clinical: A Proposed Policy Literacy Pathway In Health Sciences Education, Zarina Syed

Health Professions Education

Purpose: Health sciences graduates require not only clinical competence but also the ability to interpret, engage with, and enact policy. Yet, undergraduate curricula often emphasize technical skills, leaving gaps in policy literacy. Method: This conceptual paper proposes a scaffolded Policy Literacy Pathway to progressively integrate policy understanding across health sciences curricula. Results: Policy is framed as a dynamic, context-dependent process, aligning global ethical frameworks such as the Sustainable Development Goals with regional legislation and professional regulations. The pathway comprises four stages: Encounter, Iterative Engagement, Critical Reflection, and Policy Enactment. These guide students from initial exposure to policy issues through analysis …

Healthcare Students' Perspective On The Clinical Placement Learning Environment: A Cross-Sectional Study Of Third- And Fourth-Year Students At A College Of Health Sciences In The Uae., Wuni Abdul Razak, Fatima Alrayssi, Arwa Alkarbi, Latifa Al Saedi, Suliman Salih, Ajnas Alkatheeri, Mohamed Hassaneen, Mohd Nazmi Nordin, Mbambo Collen, Emne Hammoud, Shaik Balkhis Banu, Monika Sachdeva, Noon Kamil

Health Professions Education

Purpose: This study evaluates the clinical placement learning environment from the standpoint of Year 3 and Year 4 healthcare students in the UAE. Methods: The perspectives of healthcare students on clinical learning environments were studied using the validated Undergraduate Clinical Education Environment Measure (UCEEM). Third- and fourth-year (fifth-year pharmacy students inclusive) undergraduate healthcare students (radiography and medical imaging, emergency health, midwifery, nursing, pharmacy, physiotherapy, and psychology) (n=647) at a United Arab Emirates university were invited to participate in the study. The UCEEM contains 25 items under two overarching aspects, experiential learning and social engagement, with four subscales: learning opportunities at …

Fostering Cross-Institutional And Cross-Disciplinary Peer Review Of Teaching In Health Sciences Programs, Snezana Kusljic, Nicole B Reinke, Tania N Crotti, Nidhish Francis, Sophie Paquet-Fifield, David Van Reyk, Roma Forbes

Health Professions Education

Purpose: The education of future health professionals brings with it a diversity of programs, the requirements for effective work-integrated learning experiences, and best use of discipline and clinical expertise. Peer review of teaching (PRT) is one mechanism whereby interdisciplinary connections are built to improve the quality of teaching and learning. This article introduces a novel perspective on the PRT process and highlights the importance of interdisciplinary collaboration. Methods: The Health Sciences Special Interest Group within the Higher Education and Research Development Society of Australasia initiated a cross-institutional PRT which included seven educators of varying academic experience from six Australian academic …

Integration Of Planetary Health In Health Professions Education And Training In Low- And Middle-Income Countries: A Scoping Review, Rayishnee Pillay, Veena S Singaram

Health Professions Education

Purpose: This review explored the integration of planetary health and sustainable healthcare education in health professions education and training in low- and middle income countries (LMICs), by mapping capacity development initiatives, pedagogical approaches and contextual barriers to implementation. Method: A literature search was conducted using the following databases, MEDLINE (PubMed), EBSCOhost (Academic Search Complete), ERIC, ProQuest, Redalyc, Sabinet, Scielo, ScienceDirect, Scopus, Web of Science, and grey literature search on CORE, Google and Google Scholar for publications on the incorporation of planetary health and sustainable healthcare education in health professions education and training in LMICs between 1 January 2014 to 30 …

Professional Identity Development Among Nursing Students: The Impact Of Structured Reflective Practice, Abdelmonim El Fadely, Kamal Takhdat, Charaf-Eddine Ahraiba, Rahma Bouali, Sabah Selmaoui, Boujemaa Agorram

Health Professions Education

Purpose This study aimed to assess the impact of a structured reflective practice intervention on the development of professional identity among nursing students. Method A mixed-methods design was adopted. The intervention, based on guided reflective practice, was implemented with a voluntary sample of 58 participants. The evaluation relied on a quasi-experimental quantitative approach using the Macleod Clark Professional Identity Scale, with a comparison group and both pre-test and post-test measurements. A qualitative component was also included to explore the dimensions of the studied concept in greater depth. Results Quantitative findings showed a statistically significant difference between the post-test means (p < 0.001), indicating that the reflective practice intervention had a positive effect on the experimental group compared to the control group. The qualitative results further illuminated students’ reflections, which were organized into ten themes corresponding to the study’s conceptual framework: professional knowledge and skills, values and ethics, the nursing role, leadership, professional self-image, social image of the profession, sense of belonging, identification with role models, theory–practice integration, and career choice. These dimensions provided a comprehensive and nuanced understanding of professional identity, capturing the complexity of the concept. Conclusion These results support the integration of reflective practice as a pedagogical approach to fostering the construction and development of a positive professional identity. Professional identity, as a rich and multidimensional concept, goes beyond the process of professionalization—it represents its ultimate achievement.

Climate Change Education In Medical Curricula: Integration, Collaboration, And Preparedness For Emerging Health Challenges, Sungkono Sungkono, Agus Santoso, Firdiawan Ekaputra, Firdian Makrufardi

Health Professions Education

Climate change poses increasing risks to global health, highlighting the need for healthcare professionals who understand the links between environmental change and health outcomes. Medical education is a key platform for fostering such competencies. This study aimed to examine the role of climate change education within medical curricula, focusing on its integration, the promotion of interdisciplinary collaboration, and its contribution to preparing students for emerging health challenges. This perspective paper provides an overview of current approaches to the integration of climate change education into medical training. Sources addressing curriculum design, knowledge acquisition, practical skill development, and collaborative learning were considered. …

Undergraduate Research Experiences Toward Student Success: Challenges And Opportunities For Medical Science Students At A University Of Technology, Ziningi Nobuhle Jaya

Health Professions Education

Purpose: The study aimed to evaluate the research experiences of undergraduate medical science students at a university of technology to identify challenges and opportunities and determine the affordances of research participation for student success.

Method: The study population comprised all third-year medical science students (n=30) enrolled in the research methods module, which is the only such module offered as part of the medical science academic program. Participation was voluntary and so the final sample represents a self-selected subset of fourteen individual from the full cohort. The data collection tool comprised closed and open-ended questions evaluating challenges, lessons learned, skills developed, …

Clay Modeling As An Effective Method Of Teaching-Learning Among Second-Year Medical Students In Pathology, Seema Umarji, Padmapriya K, Sulata Kamath

Health Professions Education

Introduction: Pathology is a core subject in undergraduate medical education, but students find it challenging to understand and retain microscopic concepts through lectures alone. Clay modeling, multisensory learning strategy, is shown to enhance comprehension in other subjects but remains understudied in pathology.

Purpose: To assess augmentation of learning using clay modeling, evaluate retention of knowledge after one month and explore student perceptions of clay modeling as a teaching method.

Methods: This interventional study involved 60 second-year MBBS students in the Department of Pathology over six months. Students were randomized into study and control groups. The study group used clay modelling …

Beyond The Numbers: A Mixed-Method Evaluation Of Faculty, Peer, And Self-Assessment Using Rubrics In Blood Pressure Determination Among Phase 1 Mbbs Students, Abeetha S, Chitra M, Suba A, B. Dharani, Sadhana S

Health Professions Education

Background: Competency-Based Medical Education (CBME) emphasizes formative, learner-centred assessment. Blood pressure (BP) measurement, a core clinical skill, integrates knowledge, skills, communication, ethics, and attitude. While rubrics are widely used to standardize evaluation, their utility in triangulating faculty, peer, and self-assessment in BP measurement remains underexplored.

Aim: To evaluate the reliability and educational value of rubric-based faculty, peer, and self-assessment in BP determination among Phase 1 MBBS students using a mixed-methods approach.

Methods: A cross-sectional mixed-methods study was conducted among 98 Phase 1 MBBS students in a South Indian medical college. Forty-nine students performed BP measurement, assessed simultaneously by 5 faculty …

The Predictive Ability Of Decision-Making For Cognitive Dissonance Among Nursing Students, Adnan Yousef Atoum, Omar Atallah Al-Adamat, Emran A. Abu Aqoulah, Aya Al-Ali, Marah Al-Smadi

Health Professions Education

Purpose: The present study aimed to examine the levels of cognitive dissonance and decision-making among nursing students at Irbid National University-Irbid, Jordan, and to investigate the predictive ability of decision-making for cognitive dissonance.

Method: The study sample consisted of (251) male and female nursing students at Irbid National University-Irbid, Jordan. The cognitive dissonance scale and the decision-making scale were used after assuring validity and reliability.

Results: The study results indicated that the level of cognitive dissonance was low, and the level of decision-making ability was high. The study results also indicated that decision-making variable explained 17.2% of …

Assessing The Effectiveness Of The Well-Being Through Occupational Participation (Wbop) Intervention In Promoting Well-Being In Occupational Therapy Students From A Philippine University: A Convergent Mixed-Methods Study, Elena Wong Espiritu, Maria Concepcion Dc. Cabatan, Guia Allyza D. Rabacca, Marlowe Eriberto P. Acuña Jr, Jay Allen B. Villon, Peñafrancia E. Ching

Health Professions Education

Purpose: Filipino occupational therapy students experience decreased well-being during their educational experience. However, there is a limited amount of literature describing their well-being and a scarcity of culturally specific interventions to promote student well-being. The Well-Being Through Occupational Participation intervention is an evidence-based, manualized program consisting of a variety of occupation-based activities. The objective of this study aimed to assess the effectiveness of a modified version of the intervention in promoting undergraduate Filipino occupational therapy student well-being.

Methods: A convergent mixed-methods approach was used, specifically longitudinal survey for the quantitative design and qualitative descriptive for the qualitative design. Data was …

Design, Delivery And Impact: Practical Tips For Impactful Continuing Professional Development, Helen Mcgregor, Brett Vaughan, Louise Horstmanshof

Health Professions Education

Continuing professional development (CPD) is essential for maintaining competence and improving patient care, yet most approaches often have limited impact on practice. This Practice Brief draws on qualitative research with Australian allied health professionals to identify what motivates CPD engagement and supports meaningful learning. Participants highlighted that improving patient outcomes is the strongest driver, and that learning is most effective when it is relevant, actionable, socially interactive, and linked to real-world practice. We synthesised these insights into the PRACTICAL framework, outlining nine principles for CPD design: Patient Outcomes, Recipients, Actionable Takeaways, Co-design, Theory, Investment, Collaborative Learning, Assessment, and Link to …

Attitudes Toward Dementia Among Nursing Students: A Cross-Sectional Study, Ngoc Ha Vo Thi, Thuy Nga Tran Thi, Ngoc Sen Hoang Thi

Health Professions Education

Purpose: Dementia is the loss of cognitive function, including thinking, memory, and communication, due to brain disorders. The global prevalence of dementia is increasing, affecting younger populations. Caring for individuals with dementia requires preparedness to manage behavioral and psychological symptoms. As future healthcare providers, nursing students are expected to have the necessary knowledge and attitudes to support dementia patients. To describe nursing students' attitudes toward dementia and explore related factors.

Methods: A cross-sectional study was conducted on 520 nursing students at Da Nang University of Medical Technology and Pharmacy. A structured questionnaire assessed demographic characteristics, academic information, knowledge, and attitudes …