Digital Commons Network^™

A Comprehensive Review Of Tear Fluid Proteome Alterations In Dry Eye Disease: Insights Into Pathophysiology And Biomarker Potential, Saleh Ahmed, Steven Safille, Victoria Clifton, Shruti Sharma, Cintia S De Paiva, Ashok Sharma

Faculty, Staff and Students Publications

Tear fluid is a highly specialized and dynamic biofluid composed of proteins, lipids, mucins, and electrolytes that is essential for maintaining ocular surface integrity, immune defense, and tear film stability. Growing evidence from tear proteomic studieshas revealed extensive alterations in protein composition in dry eye disease (DED), reflecting a complex interplay between local ocular surface pathology and systemic inflammatory influences. This review summarizes proteomic findings from 50 published studies to identify tear proteins that are consistently upregulated or downregulated in DED and to interpret their biological relevance in the context of ocular surface homeostasis. Across studies, proteins upregulated in DED …

Rexinoid Net-3ib Promotes Resident Macrophage Gene Expression And Mitigates Desiccation-Induced Ocular Surface Disease, Jehan Alam, Yangluowa Qu, Jianming Shao, Ebru Yaman, Karen Zheng, Hiroki Kakuta, Stephen C Pflugfelder

Faculty, Staff and Students Publications

Purpose: To evaluate the effects of rexinoid NEt-3IB on desiccating stress-induced dry eye, as well as monocyte/macrophage gene expression and cellular trajectory.

Methods: Eyes were topically treated with rexinoid NEt-3IB (5 µM) or vehicle three times a day for 5 days of desiccating stress-induced dry eye. Single-cell RNA sequencing (RNA-seq) profiled gene expression in conjunctival immune cells. RNA-seq was also used to evaluate gene expression in lipopolysaccharide (LPS)-stimulated, dexamethasone-treated (Dex, 1 µM), or NEt-3IB-treated (1-1000 nM) cultured monocytes. Cellular state trajectory and latent time were inferred with scVelo, and latent-time-associated genes were identified using Monocle 3. Permeability to Oregon Green-labeled …

Yield Of Injury Testing For Contacts Of Children Evaluated For Physical Abuse, Lígia Batista, Joanne N Wood, Tagrid M Ruiz-Maldonado, M Katherine Henry, Jan Leonard, Angela Bachim, James D Anderst, Farah W Brink, Hannah B Sahud, Lori D Frasier, Nancy S Harper, Natalie Laub, Colleen J Bressler, Daniel M Lindberg

Faculty, Staff and Students Publications

Background: Siblings and household contacts of physically abused children are at increased risk for physical abuse and may have injuries that are not clinically apparent. However, injury testing recommendations for contact children remain debated.

Objective: Our objective was to describe testing practices and yield for contacts of physically abused children.

Participants and settings: This is a cross-sectional study of children < 10 years old evaluated for physical abuse at 10 US pediatric referral centers in the CAPNET research network.

Methods: We report the proportion of contact children who had a skeletal survey (SS) or neuroimaging and describe the proportion of tests that identified injuries.

Results: Among 6256 index evaluations, 4863 contacts were identified. Among 794 contacts with physical examinations, …

Efficient In Vivo Pharmacological Inhibition Of Δfosb, An Ap-1 Transcription Factor, In The Brain, Sean Mcneme, Anil Kumar, Yun Young Yim, Brandon W Hughes, Corey St Romain, Yi Li, Ashwani Kumar, Qichao Bao, Molly Estill, Shanghua Fan, Nadeen Takatka, Earnest P Chen, Matthew Rivera, Haiying Chen, Alfred J Robison, Mischa Machius, Stephen J Haggarty, Jeannie Chin, Eric J Nestler, Jia Zhou, Gabby Rudenko

Faculty, Staff and Students Publications

ΔFOSB, an unusually stable member of the AP-1 family of transcription factors, mediates long-term maladaptations that play a key role in the pathogenesis of drug addiction, cognitive decline, dyskinesia, and several other chronic neurological and psychiatric conditions. We have recently identified that 2-phenoxybenzenesulfonic acid-containing compounds disrupt the binding of ΔFOSB to DNA in vitro in cell-based assays, and one such compound, JPC0661, disrupts ΔFOSB binding to genomic DNA in vivo in the mouse brain with partial efficiency. JPC0661 binds to a groove outside of the DNA-binding cleft of the ΔFOSB/JUND bZIP heterodimer in a cocrystal structure. Here, we generated a …

Ins-17 Acts As A Nutrient Deprivation Signal To Mediate Adult Iis-Regulated Associative Behaviors In C. Elegans, Emily J Leptich, Priyadharshini Vijayakumar, Edward W Pietryk, Meredith I Williams, Rachana Rajupalem, Rachel N Arey

Faculty, Staff and Students Publications

Insulin/Insulin-like growth factor 1 (IGF-1) signaling (IIS) is a pleiotropic signaling pathway that functions across tissues to coordinate phenotypic changes in response to nutrient status. Thus, the ubiquity of the IIS pathway hinders efforts to elucidate the mechanisms driving specific IIS-related phenotypes. Previous research in the nematode worm C. elegans has demonstrated that loss of function of the IIS transmembrane receptor (IR) ortholog, DAF-2, results in a doubled lifespan and enhanced learning and memory behaviors in young and aged animals. However, these findings are the result of reducing DAF-2 receptor function rather than modulating ligand-receptor interactions. In the current study, …

Snapshot Usa 2024: Year 6 Of The Coordinated National Camera Trap Survey Of The United States, Brigit Rooney, Roland Kays, Michael V. Cove, Alex Jensen, Daniel J. Herrera, Camilla Price, Phuong-Thao Nguyen-Nu, Katrina Adamczyk, Peter D. Alexander, Drew R. Davis

School of Integrative Biological & Chemical Sciences Faculty Publications

No abstract provided.

Virtual Reefscapes: Modeling Fish Abundance On Artificial And Natural Reefs In Broward County, Jesse Caruana Snachkus

Undergraduate Student Symposium

This project involved constructing a VR simulation of fish abundance on artificial and natural reefs in Broward County. Two environments, SS Tracey, and a section of the Florida Reef Tract, were created to highlight ecological differences between artificial and natural reef habitats. All environments and species models were designed in Blender, a 3D modeling software, and each fish species was animated to showcase naturalistic behavior. The completed assets were then assembled in VR using the Open Brush application. This project integrates art, technology, and ecology to make marine environments more accessible and engaging for a wide audience. A combination of …

Birthdate Phaseout To A Nicotine Free Generation, Katharine Silbaugh

University of Richmond Law Review

This Article explores the Nicotine Free Generation (“NFG”) policy, an emerging endgame strategy for tobacco products that employs a completely novel legal design. Tobacco remains the leading cause of preventable death, killing almost half of its users and imposing $600 billion in social costs each year. We see two basic choices for regulating sales: prohibition or a legal age-gate, such as twenty-one, for retail sales. NFG charts a third way. Recognizing the dire harm of tobacco and the serious consequences of abrupt prohibition, NFG lays the groundwork for a gradual transition to a sales sunset. In place of the twenty-one …

Tfeb Degradation Is Regulated By An Ikk/Β-Trcp2 Phosphorylation-Ubiquitination Cascade, Yan Xiong, Jaiprakash Sharma, Meggie N Young, Wen Xiong, Ali Jazayeri, Karl F Poncha, Ma Xenia G Ilagan, Qing Wang, Bei Gao, Hui Zheng, Nicolas L Young, Marco Sardiello

Center on Aging Staff Publications

Transcription factor EB (TFEB) is a master regulator of lysosomal biogenesis and cellular clearance pathways. TFEB activity is tightly controlled by multiple post-translational mechanisms, but the exact molecular mechanism controlling its stability has remained elusive. Here, we identify the IκB kinase (IKK) complex as a key regulator of TFEB protein stability through a phosphorylation-ubiquitination cascade. A high-content kinase inhibitor screen reveals that IKK inhibition increases TFEB protein levels, and genetic ablation of IKK components increases TFEB stability, upregulates lysosomal genes, and enhances lysosomal biogenesis and degradative capacity. Mechanistically, we show that IKK phosphorylates TFEB on a cluster of serine residues …

Weaning Drives Microbiome-Mediated Epigenetic Regulation To Shape Immune Memory In Mice, Li Yang, Robert C Peery, Shirui Zhou, Xiaomin Chen, Leah M Farmer, Fabiola Gutierrez, Stephanie Fowler, Lanjing Zhang, Julia M Salamat, Karen Riggins, Jiejun Shi, Lanlan Shen

Children’s Nutrition Research Center Staff Publications

During weaning, the transition to solid food diversifies the gut microbiome, triggering a programmed immune response critical for long-lasting mucosal immunity. Previous work showed that the gut microbiome mediates epigenetic development in intestinal stem cells (ISCs) during suckling, but what happens during weaning is unclear. Here, genome-wide profiling revealed that weaning-driven microbiome changes shape the DNA methylome and transcriptome of murine ISCs in an IFNγ-dependent manner. Specifically, we observe demethylation of enhancer elements essential for MHC class II genes, which results in a transcriptional memory that persists through differentiation into adulthood. IFNγ blockade, or low-dose penicillin to target Gram-positive bacteria, …

Psychology Lecturer Confronts Ai, Attendance Decline, And Future Of Student Learning, Valerie Reynolds

Digital Edition

No abstract provided.

Zinc Oxide Nanoparticles And Chitosan Exhibited Synergistic Protection Against Diabetic Nephropathy In Mice Model, Abdulkader M Shaikh Omar, Zainab M Al Aamri, Salim M El Hamidy, Muhamed A. El Nobey

Journal of King Abdulaziz University: Meteorology, Environment and Arid Land Agriculture

Diabetic nephropathy (DN) is a progressive complication of diabetes mellitus characterized by oxidative stress, impaired glucose metabolism, and renal dysfunction. This study explored the protective effects of zinc oxide nanoparticles (ZnO NPs), chitosan, and their synergism on renal function disorders, oxidative stress, and histopathological alterations in a streptozotocin (STZ)-induced diabetic mice model. Mice treated with STZ exhibited marked hyperglycemia (284.08 ± 0.76 mg/dL), elevated HbA1c (17.35 ± 0.13%), and reduced insulin levels (3.09 ± 1.00 μI/mL), along with increased levels of renal biomarkers: urea (46.01 ± 0.28 mg/dL), uric acid (8.09 ± 0.19 mg/dL), and creatinine (1.79 ± 0.06 mg/dL). …

The Missing Lynx: Investigating Passive And Active Rewilding With The Eurasian Lynx, Isaiah Charles, Tatiana Flores, Isabella Lopez

Conservation Biology

The Eurasian Lynx (Lynx lynx) is a wild cat native to the boreal forests, mountains and grasslands of Europe and Asia (Bhattacharyya, 2025). The Eurasian lynx keep ungulate populations in check which allow for forests to recover from overgrazing, promoting biodiversity and ecological balance. However, the Eurasian lynx had become locally extinct across Europe by the 1900s due to habitat loss, hunting by farmers in retaliation for predation of livestock and the illegal fur trade. Conservation and ecological restoration efforts to rebuild the lynx population have been underway. Rewilding is a form of ecological restoration involving the reintroduction of a …

Pulling Together At A Land Conservacy: Impacting Plants, Animals, And Humans, David W. Marlow, Matt Hagen

Engage Upstate

Our project has multiple goals in multiple domains: support for the environment - both flora and fauna, a significant influx of volunteers for our community partner, experiential learning opportunities for our students, gateways to deeper SL/CE connections for both students and instructors, and enhancement of a previously uninviting (even fear-inducing) public greenspace.

• Initiated over coffee & outlined on a napkin 
• Invasive plant species removal 
• Greenway 1 mile from campus 
• Minimal training. Maximum impact 
• Freshmen/Sophomore: First look at SL/CE SPACE SPARTANBURG AREA CONSERVANCY EST1989 To promote, protect, and preserve natural resources and open space. 
• Land Management & environmental outreach 
• A response …

Constructive Falsity, Legal Truth, And The Public Interest In Not Reporting On Expunged Records, Itay Ravid

Villanova Law Review (1956 - )

No abstract provided.

Fallen Light (Video Game), Joshua Do

Presentations - 2026

Problem:

•People misunderstand Lucifer’s deception

•(shown through Temptation of Jesus in Matthew 4) •Lack of engaging ways to teach theological concepts   •Background: Lucifer corrupts not through force, but subtle self-elevation and doubt   Motivation:   •Just initially wanted to make a game of how sin entered the world for fun   •Just thought it was a cool idea in general.

Solution:

•Dialogue-driven narrative game   •6+ story chapters   •10+ regions   •Focused on spiritual conflict, deception, and discernment   •Uses event-based progression   •Presents Lucifer through subtle manipulation, by being a false light by choosing self-pleasure over what is right.

Pfas And Western Diet Effects On Adolescent Rat Feeding And Metabolism, Whesli Kim

Theses and Dissertations from DePaul University

Per- and polyfluoroalkyl substances (PFAS) are a class of widespread persistent organic pollutants associated with metabolic and neural dysfunction in humans. These processes are also affected by Western diet high sugar high fat contents. However, interacting effects of PFAS exposure and Western diet on metabolic regulation and feeding behavior have not been tested. Exposure to PFAS is high during sensitive gestational and neonatal periods of development. Adolescence is a key period where nutritional demands shift, eating habits are established, and gonadal hormones change. This study tests the hypothesis that early life exposure to PFOS alters adolescent metabolic function and feeding …

Mapping Sensory Landscapes: Distribution And Arrangement Of The Anterior Lateral Line System In Texas Catfish, Tatiana K. Flores, Ruben Tovar

Posters - 2026

Fish have a system of sensory organs known as the lateral line system. This system contains numerous mechanoreceptive neuromasts and electroreceptive ampullary organs (Northcutt et al, 2000). Neuromasts are small, hair-like structures located within canals in the lateral line system just under the fish’s skin and are open to the environment through small pores visible on its skin (Parker, 2025). Water flows into these canals through the pores which stimulates the sensitive hair cells, resulting in electrical signals sent to the fish’s brain. The brain interprets these signals and creates “images” of the fish’s surroundings, helping it to see even …

Muscular Degeneration In Caenorhabditis Elegans By Microplastic And Chemical Exposure Through Locomotion Analysis, Juan Pablo Olvera Rodriguez, Angelica Cue-Leon, David Mares

Posters - 2026

We use Caenorhabditis elegans (C. elegans) as a model organism to investigate the effects of microplastic and chemical exposure on muscle structure and function.

• Microplastics (MP) are pieces of plastic less than 5 μm, and Nanoplastics (NP) are less than 1 μm. Microplastics are man-made pollutants and have been found in the environment and in the food and water sources, including drinks from plastic bottles (Hu, 2020). • Our work relates environmental contamination to potential biological consequences at the muscular level, providing insight into how pollutants may contribute to degenerative damage.

• Polystyrene (PS) MPs alone and combined with …

Bird Population Response To Abiotic And Biotic Stresses In Urban & Restored Oak Savannah Habitat, Annabelle Ball

Posters - 2026

Birds are heavily impacted by urban development. Many experience habitat fragmentation and removal of food sources. However, other species can adapt to and even thrive in urban environments, thanks to certain tree species, bird feeders, and buildings. In North America, the house sparrow and the European starling are invasive species that benefit from urban environments (Bartelt, 2011; Fitzwater, 2014). Meanwhile, many habitats across the continent are experiencing restoration/rewilding efforts for the benefit of birds. One such habitat is the oak-savannah, which is characterized by 10-30% canopy cover and a mixture of prairie & woodland vegetation, supporting a high diversity of …

The Effects Human Presence Has On Dolphins Within A Natural Lagoon Enclosure In Managed Care, Mario Martinez, Winter Saldaña

Posters - 2026

● Previous research looked into the overall behavior of dolphins in managed care using observations of location (Dudzinski & Hill, 2025).

● This study provides opportunities to investigate the effects of human presence on dolphin activity and space use (Lauderdale et al., 2022; Trone et al., 2005).

● Purpose - To determine if human presence affects dolphin behavior / spatial use of natural lagoon.

● H1: Dolphin clustering will be seen in the most Northern part of the lagoon and area associated with humans consistently (Melillo et al., 2009).

● H2: Higher activity levels will be observed during the “during” …

(Un)Imagining India: Anti-Rape Protests As Diagnostic Of Indian State Power, Sonal Churiwal

Women, Gender, and Sexuality Studies Senior Honors Papers

Since the 2012 brutal gang rape and murder of Jyoti Singh brought India’s pervasive rape culture to international attention, media, politicians, and the broader Indian public demanded justice for “India’s daughter.” Protestors called on the Indian government to expand the police state, execute perpetrators, and create fast-track legal courts to avenge her death. Collectively, they cast the state in the role of a protector, even of a patriarchal authority. The nature of these demands stands in contrast to Kashmir and Manipur, geographies differentially controlled by the Indian state, where protests call for the withdrawal of the Indian army, a primary …

Choice, Power, & Care: Crisis Pregnancy Centers And Reproductive Justice In Missouri, Sylvie Richards

Women, Gender, and Sexuality Studies Senior Honors Papers

Crisis pregnancy centers (CPCs) are religious organizations with the purpose to dissuade people from obtaining abortions. They often present themselves as authentic reproductive healthcare clinics through medicalized language, services such as ultrasounds and pregnancy tests, promises of information on all pregnancy options, staff dressed as medical professionals, and placement near family planning clinics. In reality, CPCs are medically unlicensed and often spread medical misinformation.

No study has yet focused on CPCs in Missouri, despite providing a critical case study. The state has minimal access to abortion and other components of comprehensive reproductive healthcare and one of the worst maternal mortality …

Against A Medical Exception To Equal Protection, Govind Persad

Washington and Lee Law Review

Government regulations of medicine sometimes treat patients differently based on their race or sex. Does heightened scrutiny apply to such regulations—as is usual when law treats someone differently based on a protected characteristic? Or is there a medical exception to equal protection?

Recently, in United States v. Skrmetti, the Supreme Court upheld a law that restricted access to medical treatments for transgender minors. Though its holding did not rely on medical exceptionalism, its discussion reinvigorated interest in a “medical exception” to equal protection: that medical decisions are exempt from the searching review applied when governmental decisions consider protected characteristics.

For …

Morocco’S Liquid Gold: The Actual And Potential Role Of International Ip Law In The Protection Of The Traditional Knowledge Of Argan Oil, Christen Smith

Student Scholarship

This paper examines the actual and potential role of international intellectual property law in the protection of the traditional knowledge associated with argan oil, a product linked to the cultural heritage of the indigenous people in Morocco and increasingly commercialized globally. The paper starts with a background on the argan forest and the cultural significance of the argan tree. Then, the traditional production method used to extract argan oil is discussed. The paper also covers the rise of women’s cooperatives and how the argan oil industry has economically transformed in recent decades. The next section identifies problems in protecting traditional …

Giovanni Da Legnano’S Reception Of Thomas Of Ireland’S Manipulus Florum (1306) In The Prohemium To The Somnium, Chris L. Nighman

History Faculty Publications

This article demonstrates the reception of the influential florilegium Manipulus florum in Giovanni da Legnano’s Somnium, a discovery that has major implications for future scholarship on not only this text, but all of this author’s surviving works. It also reports some very unusual, probably unique, contextual evidence for his reception of the Manipulus.

Proceedings Of The 104th Annual Virginia Academy Of Science Meeting, 2026, Virginia Academy Of Science

Virginia Journal of Science

Proceedings (abstracts) of the 104th Annual Meeting of the Virginia Academy of Science, May 28, 2026, at Christopher Newport University, in Newport News, Virginia.

Thy Rod And Thy Staff Comfort Me, Drew Moser

2026 MFA Visual Arts

No abstract provided.

Exploratory Evaluation Of Handler Performance And Processing-Related Outcomes During Cattle Handling, Morgan Marquardt

Department of Animal Science: Dissertations, Theses, and Student Research

Animals have several critical roles in society, including the production of food and fiber, and companionship. Contact with animals has been shown to benefit human physical and mental health, yet little research has investigated the impact that humans have on animals. Handler skill may considerably affect the extent to which humans influence animal health and wellbeing, and this may be a critical consideration for animal agriculture. Objective assessment of cattle handler skill has received limited attention in the scientific literature, despite its importance to human safety and animal welfare. When compared to less skilled handlers, highly skilled cattle handlers may …

Rooted Families: A Whole Family Ministry, Aubree Nelson

Christian Ministries Senior Capstone

Rooted Families is set in the context of a family dynamic: a mother, a father, and children. Parents often drop their kids off at church on Sunday, yet many feel unqualified to disciple their children at home. This paper is the philosophy of ministry for Rooted Families, which is located at First Missionary Church in Fort Wayne Indiana. The goal is to provide a framework for churches to enable them to create spaces for parents to learn and be equipped to disciple their children. This paper describes the theological, anthropological, and sociological premises that affect parents and their influence over …