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Pbmc Transcriptome Analysis Following Lps Stimulation Reveals Distinct Inflammatory Strategies In Feed-Efficient And Inefficient Beef Cattle, Yarahy Leal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Improving feed efficiency is a primary objective in livestock production, serving as a major driver of profitability and sustainability in beef cattle operations as feed costs continue to rise. Residual feed intake (RFI) and residual average daily gain (RADG) are widely recognized indicators of feed efficiency and are commonly used as selection traits to reduce production costs and environmental impact. Previous research suggests a functional shift in transcriptional activity associated with immunity depending on efficiency phenotype, with evidence of greater immune system robustness in more efficient animals. This dissertation examines the molecular mechanisms linking feed efficiency and immune competence and …

Early Life Adversity And Reward Reactivity In Humans, Maria C. Potts

Graduate Theses, Dissertations, and Problem Reports (ETD)

Both animal and human research has identified early life adversity (ELA) as a prominent risk factor for substance use disorder (SUD). However, the underlying mechanisms linking ELA to SUD development remain unclear. Animal studies point to differences in reward responsiveness as the primary explanation; specifically, that ELA could lead to individual differences in the attribution of incentive salience to reward-predictive cues, or the “sign-tracking” behavioral phenotype. Sign-tracking has been linked to addiction-related behaviors in both animal and human studies, but no human studies have examined whether sign-tracking could partially explain the link between ELA and SUD. In fact, human studies …

The Role Of Temperature And Carbon Source In Nutrient Acquisition For Peptidoglycan Synthesis In A Gfp-Expressing Borreliella Burgdorferi, Barrett-Anne Conner Briggs

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lyme disease is the most common vector-borne disease in the United States with an estimated yearly incidence rate of more than 300,000 cases. Borreliella burgdorferi, the etiological agent of Lyme disease, is transmitted by the bite of an infected Ixodes scapularis tick and can cause debilitating pathologies affecting numerous body systems. Symptoms include fever, fatigue, joint pain, and a characteristic bull’s eye rash (erythema migrans) at the site of the tick bite. The timely and appropriate use of antibiotics effectively treats early Lyme disease; however, uncomfortable side effects reduce treatment compliance. If left untreated, long-term complications can arise including facial …

Programs Of Support For Wardship Experienced Students: Exploring Program Directors' Expectations And Experiences Through A Bioecological Framework, Adalheid Faith Crum

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this research is to explore the expectations and experiences of program directors of campus support programs which center wardship experienced students. This research question guided the current study: What expectations and experiences influenced program directors’ development and reform of a wardship-centered campus support program? This inquiry applies Ürie Bronfenbrenner’s Person–Process–Context–Time (PPCT) bioecological model. Semi-structured interviewing was utilized for this case study. In this research, six directors of campus support programs centering wardship experienced students were interviewed. Additionally, this work employs content analysis of twenty-eight National Research Collaborative for Foster Alumni and Higher Education (NRC-FAHE) publications, introducing fourteen …

Optimization Of Feed Manufacturing Productive Capacity, Feed Quality, And Feed Safety Utilizing A Variety Of Pellet Die Parameters, Feed Additives, Conditioning Specifications, And Simulated Feed Bin Storages, Lucas Edward Knarr

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Chapter 2, an experiment was conducted to determine if the inclusion of 1.50% mixer-added water (MAW) and/or 0.25% Azomite^® (AZM) would be suitable in alleviating concerns regarding pellet production rate and pellet quality when manufacturing feed with low-moisture corn. Furthermore, the authors aimed to determine if these additives had varying effects when utilizing different pellet die thicknesses. A 2 (Azomite; 0.00% or 0.25%) x 2 (mixer-added water; 0.00% or 1.50%) x 3 (pellet die thickness; 32 mm, 38 mm, or 45 mm) factorial arrangement was utilized to create the twelve experimental treatments, each manufactured …

Orogenesis, Timothy Ryan Butcher

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis and accompanying exhibition explore the translation of panenhenic mystical experience and the collaboration with Appalachian natural spaces into abstracted landscape images. This work aims to “map” a sensation of fundamental unity and gesture toward the ineffable by utilizing found-object stamp printing, and automatic mark-making. Inspired by practical mystic traditions and contemporary landscape painting, this body of work, entitled Orogenesis, combines intricate, abstracted ink landscape drawings and photographs to create images suggestive of the mystical patterns in the process.

Designing Dracula: A Feminist Revenge Fantasy, Really: A New Take On The New Woman, Spencer T. Lawlor

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis will document the process of designing the costumes for Dracula: A Feminist Revenge Fantasy, Really, by Kate Hamill. It will detail a close reading of the text and how it differs from its original source material, Bram Stoker’s Dracula, an examination of the research process for this design, as well as an analysis of the design process and execution of costumes for this production. It will explore the challenges I faced as the Costume Designer on this production, including but not limited to: examining the gender dynamics involved in genderbending a well-known piece of literature, working within a …

Computational Methods For Identification Of Molecular Signatures, Weijun Yi

Graduate Theses, Dissertations, and Problem Reports (ETD)

This work develops computational methods for identifying molecular signatures from high-throughput genomic data and for modeling long non-coding RNA (lncRNA) sub-cellular localization. The response of multiple myeloma to CB-6644, a selective RUVBL1/2 complex inhibitor with potential anti-tumor activity, is analyzed to identify drug-responsive pathways and molecular signatures. Conventional gene set enrichment analysis (GSEA) often excludes low-expression genes. Here, phenotype comparison is reformulated as a supervised machine learning problem: genes most informative for discrimination are first selected using a machine learning approach, and GSEA is then applied to these machine-learning derived gene sets. This framework improves detection of CB-6644-associated pathways. For …

Reducing Inorganic Phosphorus In Poultry Diets: Nutritional Constraints And Strategies For Successful Implementation, Elizabeth Ann Lynch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Applied poultry nutrition and feed manufacture science can be described as a “three-legged stool” supported by pellet quality, feed hygienics, and nutrient availability, where imbalance in any one component can compromise overall bird performance. The research presented herein primarily addresses feed hygienics and nutrient availability, with pellet quality serving as an underlying theme, to better understand how processing conditions and dietary formulation influence performance and nutrient utilization.

In Chapter 2, the objective of this study was to evaluate the effects of hygienizer retention time on bacterial reduction during feed manufacture using Enterococcus faecium as a Salmonella surrogate. Pellet mill motor …

A Fine-Scale Genomic Study Of Cheat Mountain Salamanders (Plethodon Nettingi) On Managed Public Land, Amylynn Nicole Ephraim

Graduate Theses, Dissertations, and Problem Reports (ETD)

Habitat fragmentation has the potential to isolate localized populations, particularly those which are understood to have limited dispersal capabilities. This isolation can reduce genetic diversity through the effects of inbreeding depression, and small populations are more susceptible to the impacts of genetic drift. In this study, I aim to understand the level of genetic connectivity between known occupied patches of Cheat Mountain salamanders (Plethodon nettingi; CMS) on managed public land. CMS are a federally threatened, high-elevation, West Virginia endemic species associated with red spruce (Picea rubens) forests. I used double-digest restriction site associated DNA (ddRAD) sequencing …

Plant Invasion Patterns In Understory And Soil Microbial Communities Of North Central West Virginia Mixed Hardwood Forests, Isabelle Marie Uganski

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive plants alter forest ecosystems by reducing species richness, habitat heterogeneity, and nutrient cycling as they rapidly displace native plants. Invasive plant composition can influence soil health through altering soil microbial associations and communities. By studying the mechanisms in which invasives alter above- and below-ground communities, we can better understand whole-ecosystem impacts and inform management decisions. This study examined the relationships between co-occurring invasion on above and belowground communities of mixed hardwood forests of north central West Virginia. Despite distinctive associations with aboveground characteristics (native diversity, native tree regeneration, and leaf litter density) we detected limited changes to soil microbial …

Accessing Campus Mental Health Services In A Nigerian Southwestern University: Barriers To Academic Success, Caroline Dada

Electronic Theses and Dissertations

Mental health disorders are a significant health concern among higher education institutions in sub-Saharan Africa, including Nigeria. A substantial treatment gap exists in the Nigerian healthcare system, and this gap is further worsened by cultural and religious barriers, which reinforce stigma that discourages students from seeking professional help. This qualitative narrative inquiry explored the barriers students encountered when accessing mental health services at a university in Southwestern Nigeria, to increase service access. Qualitative semistructured interviews were conducted with seven professional mental health providers at a university counseling center between March and April 2025. Inductive thematic analysis was used to identify …

Statistical Abstract, Fiscal Year 2026, Tennessee. Department Of Correction

Annual Statistical Abstract

This document is the annual statistical report for the Tennessee Department of Correction during the fiscal year.

For Two Cents A Pound, Manuel F. Medrano

History Faculty Publications

No abstract provided.

Heat Misers, Snow Misers, And Cognitive Misers: The 'Nukewarm' Perceptions Of Nuclear Power, Tyler Williams

Dissertations and Theses

Rising energy demand in the United States necessitates alternatives to fossil fuels, yet renewable energy sources alone are insufficient to meet this demand. Nuclear power presents a promising option but faces persistent public hesitancy. This study examines how heuristic-based biases relate to American's risk-benefit perceptions towards nuclear power. Guided by the heuristic-systematic model. Results indicate that competence and affect are related to risk-benefit perceptions, and naturalness shows no relationship with risk perceptions but does correlate with benefit perceptions. Implications highlight the importance of affect-driven communication strategies for nuclear communicators, while also underscoring the need for participatory approaches that foster trust …

Pharmacogenetic Hslco1b1*14-Guided Dosing Of Methotrexate In Transgenic Arthritic Mice Normalizes Exposure And Response., Felicia Gooden, Brennan D. Meier, Griffin D. Shaffer, Kim Gibson, Paul Toren, Nieko C. Punt, Sandhya Subash, Dilip K. Singh, Bhagwat Prasad, Laura Ramsey, Zachary L. Taylor

Manuscripts, Articles, Book Chapters and Other Papers

Juvenile idiopathic arthritis (JIA) is a chronic autoimmune disease that negatively affects ~100,000 children under the age of 16 in the United States. About ~30% of these patients fail first-line drug therapy with low-dose methotrexate (MTX) due to poor tolerability or lack of efficacy. The SLCO1B1*14 allele is associated with increased MTX clearance and has been linked to reduced overall drug exposure and nonresponse to MTX in JIA patients. Herein, we describe transgenic hSLCO1B1*14 and hSLCO1B1*1 DBA1/J mSlco1b2 knock-out mice, which we used to assess arthritic response to MTX using the collagen-induced arthritis model. Mass spectrometry-based proteomics analysis revealed that …

Bovine Lactoferrin Maintains Antibacterial Effect Against Neonatal Escherichia Coli Septicaemia Isolates Despite The Presence Of Iron Acquisition Genes, Matthew Mayorga, Joshua Wheatley, Brianna N. Maldonado, Susana Chavez-Bueno

Manuscripts, Articles, Book Chapters and Other Papers

Introduction. Escherichia coli is a dominant cause of neonatal sepsis for which no prevention measures currently exist. Lactoferrin (LF) is an iron-sequestering antibacterial protein that has been noticed to decrease neonatal late-onset sepsis.Hypothesis/Gap Statement. LF's effect on in vitro growth of neonatal E. coli clinical isolates causing septicaemia is unknown. The prevalence of iron acquisition virulence genes which contribute to pathogenicity outcomes in these strains has also not been described in detail.Aim. To evaluate bovine lactoferrin's (bLF) effects on the in vitro growth of neonatal septicaemia E. coli isolates, and to determine the presence of iron acquisition virulence …

Identification Of Neural Crest And Melanoma Cancer Cell Invasion And Migration Genes Using High-Throughput Screening And Deep Attention Networks., J C Kasemeier-Kulesa, S Martina Perez, R E Baker, Paul M. Kulesa

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Cell migration and invasion are well-coordinated in development and disease but remain poorly understood. We previously showed that the neural crest (NC) cell migratory wavefront shares a 45-gene panel with other cell invasion phenomena. To rapidly and systematically identify critical genes, we performed a high-throughput siRNA screen and statistical and deep learning analyses to determine changes in NC- versus non-NC-derived human cell line behaviors.

RESULTS: We find 14 out of 45 genes significantly reduced c8161 melanoma cell migration; four of the 14 genes altered leader cell motility (BMP4, ITGB1, KCNE3, and RASGRP1). Deep learning identified marked disruptions in cell-neighbor …

The Lens Of Farmers: Understanding Culture To Improve Occupational Therapy Care, Allison Marie Stephani

Occupational Therapy Capstones

Introduction: Accessible healthcare is essential for farmers due to their limited available time and hazardous job duties (Hildebrand et al., 2019; Hopkins et al., 2023; Salmon et al., 2025; U.S. Department of Agriculture, 2023). Due to the high incidence of injuries that occur within the profession, it is crucial that farmers seek appropriate healthcare services to address these concerns. However, a significant barrier for seeking healthcare assistance is the limited cultural competence among healthcare professionals in understanding the unique needs within farming communities (Gordon et al., 2024; Hildebrand et al., 2019; Hopkins et al., 2023; Purc-Stephenson et al., 2024). The …

Swimming With Confidence From An Occupational Therapy Perspective, Emma M. Schultz

Occupational Therapy Capstones

Background: Autism Spectrum Disorder (ASD) is a neurological disorder that impacts thousands of people and their families. Those with an ASD diagnosis demonstrate difficulty with safety awareness are at an increased risk to die due to drowning. Those with autism are often drawn to water for a multitude of reasons especially after an elopement attempt. There has been an increased awareness and development in aquatic therapy to reduce the drowning risks for those with ASD. Occupational therapists have begun to develop interventions that targets basic swimming and water safety. During these interventions, occupational therapists use their skills of activity analysis, …

Harvesting Health: Cultivating Skills For Wellness In Agricultural Workers, Katie Renee Quinley

Occupational Therapy Capstones

Purpose: The nature of agricultural work is physically demanding and associated with an increased risk of musculoskeletal injury due to repetitive movements, heavy lifting, awkward postures, and prolonged physical labor. Despite the importance of agriculture as a meaningful occupation and major contributor to the workforce, limited resources address the unique physical demands of agricultural labor. The purpose of this scholarly project was to develop a video-based educational resource to promote safety and continued occupational participation in agricultural workers. Additionally, the project aims to assist therapy professionals in implementing ergonomic and task modifications through an accompanying resource guide.

Methodology: A literature …

The Missing Link: Psychosocial Integration In Neuro Ot Care, Emily Nelson

Occupational Therapy Capstones

Introduction: Over 1 in 3 people are affected by neurological conditions, making it the leading cause of illness and disability worldwide (World Health Organization, 2024). Patients are often referred to inpatient rehabilitation to increase independence and functional recovery. There is a need to address psychosocial factors within this setting thus, The Missing Link: Integrating Psychosocial Factors into Neuro OT was created.

Methodology : The capstone is guided by the Model of Human Occupation (MOHO), which emphasizes the dynamic interaction between the person and their environment and its influence on psychosocial factors, personal identity, and competence. A comprehensive needs review was …

Pathways To Participation: Enhancing Inclusion For Individuals With Idd, Katie Koxlien

Occupational Therapy Capstones

Background: Youth with intellectual and developmental disabilities (IDD) continue to experience barriers to meaningful community participation despite policies promoting inclusive community-based services (Friedman, 2017). Sensory sensitivities, difficulty with transitions, and overstimulating environments frequently limit engagement and increase anxiety or behavioral responses when appropriate supports are not present (Bagatell et al., 2022; Dean et al., 2018; Tint et al., 2017). Research indicates that inclusion requires intentional programming that incorporates structured supports, consistent routines, and opportunities for meaningful engagement (Edwards et al., 2021; Hall, 2016).

Methods: The purpose of this scholarly project was to develop a week long sensory exploration camp for …

Intermittent Phrenic Afferent Activation Induces Phrenic Motor Plasticity, Nicole M. Popp, Taylor C. Holmes, Elizabeth A. Rozycki, Kristi A. Streeter

Physical Therapy Faculty Research and Publications

Approaches such as electrical stimulation of the phrenic neuromuscular system are currently used in humans to manage breathing impairments. We recently showed that electrical stimulation of the diaphragm muscle induces phrenic afferent-dependent plasticity. These findings highlight the need to better understand the mechanisms of afferent-mediated plasticity to guide and improve the development of novel therapies using electrical stimulation. Here, we tested the hypothesis that intermittent activation of large-diameter phrenic afferents induces phrenic motor plasticity, which, like other forms of plasticity, is pattern sensitive. We delivered inspiratory-triggered electrical stimulation using a narrow pulse width (0.1 ms) to preferentially activate large-diameter afferents, …

Synergistic Sensitization Of Pancreatic Cancer Cells By Nanosecond Pulsed Electric Fields And Cold Atmospheric Plasma Via Amplifying Ros And Apoptotic Signaling, Zobia Minhas, Edwin A. Oshin, Lifang Yang, Chunqi Jiang, Siqi Guo

Bioelectrics Publications

Pancreatic cancer remains a highly lethal malignancy, with standard therapies offering limited benefits in advanced stages; thus, novel strategies that exploit specific cancer cell vulnerabilities are urgently needed. Building on our previous findings that nanosecond pulsed electric fields (nsPEF) combined with cold atmospheric plasma (CAP) produce enhanced cytotoxicity, this study investigates the molecular mechanisms underlying this synergy. Pan02 pancreatic cancer cells were subjected to nsPEF, CAP, or a combination of both. We assessed cell viability, reactive oxygen species (ROS) production, and mitochondrial integrity using metabolic assays, flow cytometry, and fluorescence microscopy. Apoptotic markers were evaluated via Western blotting and caspase …

Targeting Ptp4a3 And Kvx-053 Mitigates Alcohol-Amplified Sars-Cov-2 Spike Protein-Induced Acute Lung Injury, Pavel A. Solopov, Ruben Manuel Luciano Colunga Biancatelli, Elizabeth R. Sharlow, John S. Lazo, John D. Catavas

Bioelectrics Publications

Introduction: Chronic alcohol exposure is associated with increased vulnerability to acute respiratory distress syndrome (ARDS), and emerging evidence suggests that this susceptibility extends to the severe respiratory manifestations of COVID- 19. The SARS-CoV-2 Spike Protein S1 subunit (S1SP) can independently trigger robust inflammatory and vascular injury responses in the lung, and our prior work demonstrated that chronic alcohol consumption markedly intensifies these effects in mice. PTP4A3 phosphatase is a key regulator of inflammation and endothelial barrier function. In this study, we investigated whether the PTP4A3 inhibitor KVX-053 can counteract alcohol-enhanced S1SP-induced lung injury in K18-hACE2 mice.

Methods: Animals were maintained …

Nanosecond Electric Pulses As A Novel In Situ Vaccination Strategy For Cancer Treatment: Mechanisms, Challenges And Prospects, Siqi Guo

Bioelectrics Publications

Nanosecond electric pulses (nsEPs) are an emerging pulsed-power technology with unique bioelectric characteristics distinct from conventional long-pulse electroporation. As a tunable physical modality, nsEPs can modulate intracellular structures, membrane dynamics, and signaling pathways. Increasing evidence supports nsEPs as a promising non-thermal tumor ablation approach due to their high spatial precision, preservation of critical tissue structures, and minimal adverse effects. One of the most significant discoveries associated with nsEP tumor ablation is the induction of potent systemic antitumor immunity, particularly in situ vaccination (ISV) effects and, in some cases, abscopal effects against distant untreated tumors. Substantial evidence demonstrates that nsEPs can …

Hsp90 Inhibitor, At13387 Mitigates Chronic Lung Injury In Pre-Pubertal Mice: A Therapeutic Axis In Bronchopulmonary Dysplasia, Pavel A. Solopov, Christiana Dimitropoulou, John D. Catravas, Ruben M. L. Colunga Biancatelli

Bioelectrics Publications

Exposure to hydrochloric acid (HCl) can provoke severe chronic pulmonary injury. Children are particularly vulnerable due to their smaller airways, higher respiratory rate, and stronger inflammatory response; yet no countermeasures exist for HCl-induced chronic lung injury in the pediatric population. We have previously demonstrated the involvement of HSP90 during HCl-induced lung injury in pre-pubertal (p24) mice, who develop stronger persistent inflammation with higher NLRP3 inflammasome activation, but less pulmonary fibrosis compared to adults. Here, we tested the hypothesis that post-treatment with the second-generation HSP90 inhibitor AT13387 (Onalespib), administered subcutaneously beginning 24 h after HCl instillation, would prevent HCl-induced chronic lung …

Using Maximum Entropy (Maxent) Tool In Arcgis Pro For Species Distribution Modelling Of Pseudoscorpions In The Pacific Northwest, Elliot Toshihiro Cochran

Masters in GIS Practicum Reports

The use of Geographic Information Systems (GIS) to create Species Distribution Models (SDMs) is rising among many scientific disciplines. Many professionals who were not originally focused on GIS are increasingly becoming more invested in this technology. SDMs are widely used to estimate species’ potential geographic distributions and to evaluate the environmental factors that constrain their ranges. The presence-only maximum entropy (MaxEnt) algorithm implemented in the MaxEnt tool in ArcGIS Pro was used in this project to model habitat suitability for two species of pseudoscorpions, M. pacificus and an undescribed species, in Oregon and Washington. Presence points (of specimen sample locations) …

Front Matter

Lewis & Clark Law Review

Front Matter contains Table of Contents, Masthead, and faculty for Lewis & Clark Law Review Volume 30, Issue 1.