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Development Of Shigella Flexneri Detection Method By Real-Time Polymerase Chain Reaction Targeting The Sfmd Gene, Muktiningsih Nurjayadi, Puan Aqila Azizah, Agus Setiawan, Ananda Indah Putri Sihombing, Anisa Fitriyanti, Grace Grace, Gusti Angieta Putri, Helzi Angelina, Royna Rahma Musie, Jefferson Lynford Declan, Gladys Indira Putri, Dandy Akbar Juliansyah, Siti Fatimah, Tiara Fahriza, Irma Ratna Kartika, Fera Kurniadewi, Novitasari Novitasari, Bassam Abomoelak, Hesham Ali El Enshasy, Shyi-Tien Chen

Makara Journal of Science

Shigella flexneri can cause shigellosis, which results in high fever, vomiting, diarrhea, or death. This study developed a detection method based on real-time polymerase chain reaction that targets the sfmD gene of S. flexneri given that the virulence factor encoded by this gene is thought to facilitate adhesion to a host surface. The proposed method involves primer design, DNA isolation, the optimization of primer annealing temperature, and confirmation, specificity, and sensitivity tests. A sfmD primer with an amplicon length of 155 bp was annealed to the target DNA at an optimized temperature of 60 ℃ (range 54 °C–62 °C). This …

What Bereaved People Have Found Helpful Following Death Of A Loved One In A Hospital: A Survey Study, Jon Swanson Phd, Mindy Flanagan Phd, Rachel Pfafman Mph, Mba

Other Specialties

Given the individual and public health impact of bereavement, discovering what people perceive as helpful after loss can improve interventions. In contrast to national projects, this project focused on two hospitals in a Midwestern hospital system.

A survey was developed to assess the work of chaplains with next of kin following a death, to assess the awareness and helpfulness of persons and organizations in the community, and to ask what people had done, said, or given that was helpful, or what respondents wished people had done. From a list of 283 people invited to respond, 52 completed the survey.

Survey …

An Exploratory Approach For Teaching Systems Of Linear Odes With Real Repeated Eigenvalues, Biyong Luo, Li Zhang

CODEE Journal

In this paper, we present a project that aims to pique students' interest and facilitate their engagement in learning. Students will be encouraged to take an active role in their learning of systems of linear ODEs with real repeated Eigenvalues. Through discussion and completion of a set of guided questions given in the project, students will gain a deeper understanding of the topic. Also, students will use the technology tool Bluffton App to draw solution trajectories in a phase plane, and then learn necessary skills for explaining the movements of the trajectories and their long term behaviors. This project may …

Revisiting Underwater Image Enhancement For Object Detection: A Unified Quality–Detection Evaluation Framework, Ali Awad, Ashraf Saleem, Sidike Paheding, Evan Lucas, Serein Al-Ratrout, Timothy C. Havens

Michigan Tech Publications

Underwater images often suffer from severe color distortion, low contrast, and reduced visibility, motivating the widespread use of image enhancement as a preprocessing step for downstream computer vision tasks. However, recent studies have questioned whether enhancement actually improves object detection performance. In this work, we conduct a comprehensive and rigorous evaluation of nine state-of-the-art enhancement methods and their interactions with modern object detectors. We propose a unified evaluation framework that integrates (1) a distribution-level quality assessment using a composite quality index (Q-index), (2) a fine-grained per-image detection protocol based on COCO-style mAP, and (3) a mixed-set upper-bound analysis that quantifies …

Penggunaan Metode Socrates Dalam Mengembangkan Pendidikan Tinggi Hukum Di Indonesia, Syarifah Lisa Andriati, Inneke Kiki Rizki, Ain Najwa Binti Mohd Malian

Jurnal Hukum & Pembangunan

The ability to think critically and have the ability as a problem solver is needed in higher education Law in Indonesia. One of the methods used is the Socratic Method. Critical thinking is one of the aspects that law students must have to improve their ability as problem solvers. This research was conducted to contribute that the application of the Socratic method is effective to be applied in the learning process of higher education in Indonesia, as well as to provide evaluation and knowledge about the challenges and obstacles that will be faced in the process of applying the Socratic …

Mogok Kerja Tenaga Medis: Problematika Pada Uu Ketenagakerjaan Dan Pilihan Model Pengaturannya, Rama Agung

Jurnal Hukum & Pembangunan

The issue of healthcare workers' strikes remains complex in terms of regulatory frameworks. On one hand, there is a conflict of interest between public interest in access to healthcare services and, on the other, the Labor Law, which regards the right to strike as a fundamental right of workers, integral to collective bargaining. This paper examines the challenges in regulating healthcare workers' strikes by analyzing Article 139 of the Labor Law and exploring regulatory models that support the provision of high-quality healthcare services. The findings highlight several key points. First, Article 139 of the Labor Law does not yet provide …

Hak Yang Mengering: Kuasa Modal Terhadap Hak Atas Air Di Cikeusal, Syahwal Syahwal, Muhamad Nabil Gunawan

Jurnal Hukum & Pembangunan

This paper originates from an observation of the water crisis affecting Cikeusal. Recognizing water as an integral part of human rights, we pose the question: why does a water crisis occur in Cikeusal, despite water being recognized as a human right? In addressing this issue, we position water as a resource closely intertwined with political dynamics. Therefore, we employ a socio-legal approach, using autoethnography as the primary method for data collection. Our study reveals that the water crisis in Cikeusal stems from capitalist expansion in the form of extractive industries, specifically karst mining. This capitalist expansion has led to the …

Lahirnya Tensi Metafisik Di Urat-Leher Projek-Projek Interpretasi Konstruktif, Herman Bakir, Mukhtasar Syamsuddin

Jurnal Hukum & Pembangunan

This project is a legal hermeneutics. The aim is to elaborate the paradigm of the law as integrity theory, Ronald Dworkin’s work, which has received recognition as one of the most influential philosophical systems in the world of law throughout the last century. The issues discussed are divided into three subjects: (1) the interpretation role in legitimizing “law” as a corpus that has a “soul”; (2) a concept of interpretation from the womb of the law as integrity theory; (3) the dual capacity of “constructive interpretation” in [a] “returning” law to its roots; and [b] housing “morals” within the body …

Synthesis Of Schiff Base Based On Aminopolysaccharides A Chitosan Apis Mellifera With Salicylic And Glutaraldehydes, Alisher Mengliev

Technical science and innovation

Chitosan is a linear aminopolysaccharide. Using methods of chemical modification of chitosan, a number of new biologically active materials have been created. The main interest in these products is due to the preservation of the basic physicochemical and biological properties of the original polymer and the acquisition of new properties depending on the nature of the introduced groups. Modified chitosan can be used in various fields such as drug delivery, textiles, wastewater treatment and a number of biomedical applications. The purpose of this work is to extract chitosan from the remains of ApisMellifera bees and synthesize a Schiff base with …

Development Of Technical Solutions To Prevent The Formation Of Sludge Regime At The Bottom Of A Well, Rustam Umarkhanovich Juraev, Jonibek Tursunov

Technical science and innovation

The trouble-free process of drilling wells largely depends on the effective cleaning of the borehole. The formation of a slurry regime at the bottom of the well leads to the process of repeated crushing of drilling fines, which in turn leads to a decrease in the mechanical drilling speed, to an increase in the energy consumption of the rock-crushing tool for repeated crushing, and also increases the risk of oil seals. In addition, the formation of a slurry regime at the bottom of the well increases the intensity of abrasive wear of the drilling rig. This article provides a theoretical …

Lithological-Stratigraphic Characteristics And Prospects For Oil And Gas Potential Of The Mesozoic-Paleozoic Deposits Of The Muynak Uplift, Nursultan Sakenovich Kayupov, Marina Georgiyevna Yuldasheva, Shakhnoza Ulzhavaevna Eshmetova

Technical science and innovation

To increase hydrocarbon reserve growth, it is necessary to involve areas in the immediate vicinity of established oil and gas accumulation zones in geological exploration, as they developed under similar geological conditions within a single sedimentary basin. In the central part of the Ustyurt oil and gas region, deposits have been discovered in Jurassic sediments within the Berdakh swell, adjacent to the east by the Muynak Uplift. Analysis of available geological and geophysical data, including the study of lithological, stratigraphic, and reservoir properties, allows us to predict the oil and gas potential of Mesozoic rocks in this part of the …

Mathematical Modeling Of The Atmospheric Water Harvesting Process Using Cfd, Jasur Esirgapovich Safarov, Shakhnoza Abduvaxitovna Shaxnoza, Doston Ishmuxammat Ugli Samandarov, Azamat Bakir Ugli Usenov, Shahzod Faxriddin Ugli Dostqobilov

Technical science and innovation

This article presents the physical principles of Atmospheric Water Harvesting (AWH) technologies, including spectral radiation balance, air-sorbent interactions, thermodynamic constraints, and computational fluid dynamics (CFD) modeling approaches. The study provides models of water condensation, adsorption-desorption processes, and energy consumption. In addition, a detailed thermodynamic analysis was carried out to determine the partial vapor pressure, cooling demand, and theoretical energy limits of water extraction. The adsorption process is described using Langmuir isotherms, while desorption is represented through a kinetic model dependent on activation energy. To analyze the coupled processes of airflow, mass transfer, and heat transport, CFD models based on the …

Algorithm For Stabilizing The Reference Trajectory Of Self-Tuning Systems With A Reference Model, Isamidin Hakimovich Sidikov, Feruzakhon Botirxon Qizi Sodiqova

Technical science and innovation

The paper addresses the problem of stabilizing self-tuning systems using adaptive control methods based on a reference process model. As the optimality criterion, the functional of maximum speed of response is selected. The algorithm for synthesizing the self-tuning system is based on a relay-linear control law, which possesses the property of invariance to small disturbances. The issue of ensuring the practical stability of the system under adaptive and multiplicative disturbances is examined. An algorithm for the synthesis of a reference trajectory stabilization system has been developed on the basis of a quasi-optimal passive self-tuning system (STS) with a reference model, …

Composition Of Essential Oil From Dried Basil (Ocimum Basilicum L.), Ajargul Abdiganievna Mambetsheripova, Shakhnoza Abduvaxitovna Sultanova, Jasur Esirgapovich Safarov

Technical science and innovation

Ushbu ishda maydalangan rayhon (Ocimum basilicum L.) o‘simliklaridan olingan efir moylarini (EM) ajratish va tahlil qilishga oid metodologik yondashuv bayon etilgan. Rayhon efir moyi klassik Klevenger apparati yordamida uch soat davomida gidrodistillyatsiya usulida ajratib olindi. Olingan distillyatlar n-geksan bilan ishlov berildi, so‘ngra qoldiq namlikni yo‘qotish maqsadida suvsiz natriy sulfat yordamida degidratatsiya qilindi. Ushbu sharoitda efir moyining chiqishi 0,74–0,81 % oralig‘ida bo‘ldi. Izolyatsiya qilingan moylar rang jihatdan och sariq, harakatchan suyuqliklar bo‘lib, rayhonga xos o‘ziga xos aromatik hidga ega ekanligi aniqlandi. Kimyoviy komponentlarni aniqlash uchun efir moyining sifat va miqdoriy tarkibi gaz xromatografiya-mass-spektrometriyasi (GXM-S) usulida o‘rganildi. Tahlillar Agilent 5975C inert MSD/7890A …

Optimization Of The Operational Efficiency Of Transport And Technological Complexes Based On Simulation Modeling, Kamoliddin Juraboevich Rustamov, Gulom Gaffurdjanovich Atabaev

Technical science and innovation

The article presents a comprehensive analytical approach to improving the operational efficiency of transport and technological machines (TTM) based on simulation modeling. The proposed model integrates the economic and reliability characteristics of maintenance (TM) strategies by describing the operation of aggregates, the probability of failures, and transitions between states — operational (W), faulty (F), and restored (R) — as probabilistic processes. Model parameters are identified using statistical data from real operating conditions and adapted to various types of technical systems. Computer simulation experiments are carried out under multiple scenarios of maintenance periodicity, workload intensity, spare parts resource, and repair policy. …

Study Of The Influence Of Material Heterogeneities In Humidity Measurements In A Closed Volume, Hulkar Asadullayevna Usmanova, Asadulla Turgunbaev, Umid Uralovich Xudaykulov

Technical science and innovation

The article presents generalized theoretical studies of the errors arising in the conversion of material moisture into informative parameters of the microwave field. Such informative parameters of a material include structural inhomogeneities, non-uniform moisture distribution throughout the volume and by the forms of moisture bonding, density, temperature, and mass. The influence of material inhomogeneities during moisture measurements in an enclosed volume on the errors of primary measuring transducers is investigated, along with the errors accompanying the conversion of moisture into the dielectric characteristics of the material and the conversion of dielectric characteristics into informative parameters of the microwave field. A …

Comprehensive Assessment Of The Impact Of Hydrogeological Factors On The Stability Of Open-Pit Slopes, Vokhid Rakhimovich Kadirov, Khurshida Yunusovna Norova, Zavkiddin Yahyo Oglu Ravshanov, Jamol Hasankhonovich Abdumalikov

Technical science and innovation

This paper presents an analysis of the influence of hydrogeological conditions on the stability of open pit slopes located within zones of tectonic disturbance. Based on field investigations and long-term hydrogeological monitoring conducted within the Almalyk ore cluster (Kalmakyr, Yoshlik-I, and Kurgashinkan deposits), the key factors affecting slope stability have been identified. It has been established that groundwater inflows from fractured and karstified Paleozoic rocks, as well as the presence of flooded abandoned pits, significantly increase the risk of slope deformation and failure. A special role is attributed to tectonic faults, which act as filtration channels for groundwater, leading to …

Implementasi Pelaksanaan Program I-Help (Integrated Help Promoting School) Pada Sekolah Menengah Pertama Di Jakarta, Rachma Dhiyaa' Arroyyan, Sarah Handayani

Perilaku dan Promosi Kesehatan: Indonesian Journal of Health Promotion and Behavior

Background. The I-HELP (Integrated Help Promoting School Programs) initiative is a school-based behavioural change intervention that integrates health values and belief-related variables into daily school activities. This program was developed as a follow-up to the collaboration between Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA) and SEAMEO RECFON.

Aim. his study aims to describe implementation of the I-HELP (Integrated Help Promoting School Programs) at the junior high school level in Jakarta in 2022.

Method. This qualitative research employed a case study design conducted at SMP Muhammadiyah 4 and SMP Muhammadiyah 36 Jakarta. Data collection techniques included in-depth interviews, focus group discussions (FGDs), …

Social Learning Theory Dan Status Merokok Pelajar Smp Negeri, Alya Putri Ansyari, Nur Rohmah, Lies Permana, Annissa Nurrachmawati, Rina Tri Agustini

Perilaku dan Promosi Kesehatan: Indonesian Journal of Health Promotion and Behavior

Background. The prevalence of adolescent smokers aged 10–18 in Indonesia increased from 7.2% in 2013 to 9.1% in 2018. Approximately 70 million people in the country are active smokers, with 7.4% of them aged 10–18 years.

Aim. This study aims to investigate the association between knowledge, attitude and subjective norms with smoking status among students at a public junior high school in Samarinda.

Method. This study uses a cross-sectional design with a quantitative approach. A total of 159 respondents were randomly selected using proportionate stratified random sampling. Data were collected through interviews using a structured questionnaire and …

Efikasi Diri Dalam Melakukan Diet Sehat Pada Masyarakat Desa Cipacing, Sumedang, Hilmi Khairun Nisa, Neti Juniarti, Adelse Prima Mulya

Perilaku dan Promosi Kesehatan: Indonesian Journal of Health Promotion and Behavior

Background. Noncommunicable diseases (NCDs) account for 57 million deaths (71%) worldwide and 73% of all deaths in Indonesia. Eating habits are one of the factors contributing to the incidence of NCDs. In the process of improving a healthy lifestyle, including a healthy diet, self-confidence or self-efficacy in maintaining a healthy diet is necessary.

Objective. This study aims to identify the level of self-efficacy in maintaining a healthy diet among the community in Cipacing Village, Sumedang.

Methods. This study was conducted using a quantitative descriptive research design among the community of Cipacing Village, Sumedang Regency, West Java. The sample was …

Hubungan Persepsi Manfaat Terhadap Kesadaran Kesehatan Pengguna Wearable Device Di Kota Banda Aceh, Raihanil Fitri, Tahara Dilla Santi, Hafnidar A. Rani

Perilaku dan Promosi Kesehatan: Indonesian Journal of Health Promotion and Behavior

Background. Wearable devices are portable technologies designed to be worn directly on the body and capable of collecting and analyzing data related to various aspects of a user's health. Aim. This study aims to analyze the influence of perceived benefits on users' health awareness among wearable device users in Banda Aceh City.

Aim. This study aims to analyze association of perceived benefits on users' health awareness among wearable device users in Banda Aceh City.

Method. This research applied a descriptive quantitative design with a cross-sectional approach. Data were collected using a questionnaire from 106 active wearable device …

Pengaruh Pendidikan Pencegahan Kekerasan Seksual Berbasis Animasi Terhadap Pengetahuan Dan Sikap Remaja, Lulu Lutpia Muharomah, Irma Darmawati, Asih Purwandari Wahyoe Puspita

Perilaku dan Promosi Kesehatan: Indonesian Journal of Health Promotion and Behavior

Background. Sexual violence is a major problem that impacts the physical and psychological health of adolescents. Prevention efforts are needed through health education that is in accordance with the characteristics of adolescents. Animation media is considered effective because it can convey messages audio-visually and attractively.

Purpose. This study aims to assess the influence of Animation-Based Education on adolescents' knowledge and attitudes regarding the prevention of sexual violence.

Method. The study used a one-group pretest–posttest design with 59 student respondents in Cimahi City. The intervention was in the form of an educational animated video on sexual violence prevention of ±5 minutes …

Perbedaan Faktor Lingkungan Sosial Dan Perilaku Seksual Pranikah Pada Siswa Sma Perkotaan Dan Perdesaan Di Samarinda, Syaniah Syaniah, Annisa Nur Rachmawati, Lies Permana, Nur Rohmah, Rina Tri Agustini

Perilaku dan Promosi Kesehatan: Indonesian Journal of Health Promotion and Behavior

Background. Based on the 2023 GSHS data, 7.1% of adolescents aged 13–17 in Indonesia have had sexual intercourse, of which 66.2% are male and 33.8% are female. Adolescent premarital sexual behaviour is influenced by internal and external factors. Differences in social determinants may lead to behavioural variations between groups within a population.

Aim. To identify differences in peer influence, parental roles, and mass media (as social environmental factors), as well as premarital sexual behaviour among urban dan rural high school students in Samarinda

Method. This comparative cross-sectional study involved 274 respondents, consisting of 206 respondents from three urban high schools …

Korelasi Antara Intensitas Doomscrolling Konten Negatif Kehamilan Di Media Sosial Dengan Tingkat Stres Dan Kecemasan Ibu Hamil, Besse Aismaria Am, Yudiarsi Eppang B, Jusniati C

Perilaku dan Promosi Kesehatan: Indonesian Journal of Health Promotion and Behavior

Background.The rapid development of social media has significantly influenced information-seeking behavior, including among pregnant women. One emerging phenomenon is doomscrolling, defined as the excessive consumption of negative information, which may trigger stress and anxiety.

Aim. This study aimed to analyze the correlation between the intensity of doomscrolling of negative pregnancy-related information on social media and levels of stress and anxiety among pregnant women in Pare-Pare City.

Method. This study employed a quantitative method with a cross-sectional design. The study population consisted of all pregnant women attending antenatal care at public health centers in Pare-Pare City, …

Evaluating The Impact Of A Language Translation Standard Operating Procedure For The Multi-Professional Team Working In A Maternity Care Service, Fiona Howard

South West Clinical School Journal

No abstract provided.

Improving Adoption Of Evidence-Based Guidelines On Exercise For Depression In An Adult Community Mental Health Team, Jack Richards

South West Clinical School Journal

No abstract provided.

Jbi Evidence Implementation Project: Developing An Educational Intervention For Frozen Shoulder, Laura Tapp

South West Clinical School Journal

No abstract provided.

Advanced Practitioner’S Skills In Communicating Bad News To Patients: A Service Evaluation Project Proposal, Olga Richardt

South West Clinical School Journal

No abstract provided.

Co-Designing A Staff Intervention To Provide Meaningful Activities For Patients On An Acute Older People’S Ward, Niyatiben Bhoi

South West Clinical School Journal

No abstract provided.

Collaboration And Co-Design: Utilising Patient And Public Involvement In The Development Of A Patient Information Leaflet For Patients With Frozen Shoulder, Laura Tapp

South West Clinical School Journal

No abstract provided.