Digital Commons Network^™

Perceived Obligations Of Older Partners In Living-Apart Together Relationships, Allison M. Martin

Human Development and Family Sciences Undergraduate Honors Theses

Older and middle-aged adults are increasingly in Living-Apart-Together (LAT) relationships characterized by committed romantic relationships without marriage and cohabitation. Because of assumptions that older adults will receive family caregiving as they grow older and need caregiving, it is not clear how people are likely to perceive obligations to provide caregiving assistance. As adults move into old age and incidence rates of Alzheimer’s Disease or Related Dementia (ADRD) increase, questions about the availability of family members to provide needed assistance are complicated by LAT relationships. The purpose of this study was to examine perceived obligations to provide assistance to older adults …

Gastric Sarcina Ventriculi: A Report On Two Cases, Yaomin Chen, Yu Liu, Zhiyan Fu

School of Medicine Faculty Publications

: Sarcina ventriculi is a rare Gram-positive coccus that thrives in acidic environments such as the human stomach. It has been increasingly identified in individuals with delayed gastric emptying and has been reported in association with various gastric disorders. However, its exact pathogenic role is not fully understood and remains controversial. : We present two cases of patients, one with a small bowel obstruction and the other with epigastric pain, both diagnosed with Sarcina ventriculi infection by histological examination of gastric biopsies. The patients were managed with a combination of antibiotics and a proton pump inhibitor, resulting in symptom resolution …

Role Of Cellular Proteins In The Regulation Of Herpes Simplex Virus Type 1 Gene Expression And Dna Replication, Joseph R. Heath

Electronic Theses and Dissertations

Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen that infects the majority of the human population. To facilitate infection, this virus relies on a combination of host and viral factors. Previous studies have revealed a number of host proteins that associate with the HSV-1 genome throughout infection but their putative role(s) in this process remain undefined. This study aims to examine two such proteins to determine their contributions to HSV-1 gene expression and DNA replication. The first of these factors is Ints3, a subunit of the Integrator complex. This protein complex functions in transcription regulation, and Ints3 …

Exploring Variability Of The Cg18562578 Methylquant Assay For The Forensic Discrimination Of Monozygotic Twins, Sydney E. Arnold

Student Theses

Monozygotic (MZ) twins possess identical DNA sequences, posing a problem for forensic scientists, as they are incapable of distinguishing MZ twins from each other using traditional DNA identification methods. However, the epigenomes of MZ twins will differ and might be used for their forensic discrimination. Here, we explored the variability of an assay previously developed by our laboratory that showed high discrimination potential for MZ twins using buccal swab DNA, cg18562578 MethylQuant. We measured precision at each step: bisulfite conversion, nested PCR, and qPCR, reproducibility, and repeatability. DNA methylation ratios were compared between two analysts carrying each step using the …

Cg18562578 Methylquant Assay For The Discrimination Of Monozygotic Twins, Amber P. Chauca

Student Theses

Monozygotic (MZ) twins have identical DNA, making traditional profiling methods insufficient in their discrimination. However, MZ twins can be differentiated by their levels of DNA methylation. Our research group previously developed a methodology to detect cg18562578 DNA methylation levels in MZ twins based on a two- step PCR approach and a cg18562578 MethylQuant Assay. That study generated data for only 64% of the samples it investigated, and showed a discrimination power of 77% to discriminate between MZ twins. Here, we used the same methodology but a different approach to determine DNA methylation in 73 previously unsuccessful twin pairs. We diluted …

Further Investigation Of Environmental Factors On Rapid Shear In Fibers Of A Polyester-Cotton Blend, Daniel K. Oh

Student Theses

Rapid shear is a process that occurs in thermoplastic materials when they interact with a high-velocity impact. In forensics, these interactions would typically be from a bullet interacting with clothing that consisted of synthetic fibers. When the interaction occurs, bulb-like shapes appear on damaged fiber ends, distinguishing them from other kinds of damage. The reason why these shapes occur is varied, but one prevailing factor seems to be the heat generated by the impact.

In prior experiments, the factor of temperature being important to the formation of the phenomenon, different environmental simulations that affected the sample’s temperature were done on …

Prevalence And Risk Factors Of Gastroesophageal Reflux Disease Among Population Of Saudi Arabia: A Meta-Analysis, Waleed D. Khubzan, Dana O. Kaddah, Ola E. Alkhoshiban, Mirza H. Alkhater, Botool A. Alshahrani, Rama A. Alhatlani, Muntaha S. Alsulaimani, Dalal A. Elyas, Majed A. Almourgi

Saudi Medical Journal

ABSTRACT Objectives: To find how common gastroesophageal reflux disease (GERD) is in Saudi Arabia and to identify the main risk factors linked to it. Methods: We followed PRISMA guidelines and registered the study on PROSPERO (CRD42025637142). A full literature search was done across PubMed, MEDLINE, and Google Scholar up to December 2024. Two reviewers independently reviewed the results. We assessed the risk of bias using the JBI critical appraisal tool and evaluated potential reporting bias through Egger’s test. To determine the overall prevalence and key contributing risk factors, we conducted a meta-analysis utilizing a random-effects approach. We also did subgroup …

Unified Mathematical Modeling, Analysis And Simulation Of Covid-19 And Ecological Processes, Jacob Derrick

Masters Theses and Doctoral Dissertations

This dissertation is concerned with the modeling, simulation, and analysis of complex population dynamics using compartmental modeling frameworks based on ordinary differential equations (ODEs). First, we propose a mechanistic model to investigate the transmission dynamics of COVID-19, particularly focusing on the emergence and population-level impact of a post-acute sequela referred to as long COVID and vaccination effects. We then move forward with a model that incorporates multiple strains and reinfection dynamics. By fitting these models to epidemiological data from the United States and the United Kingdom, we conduct both mathematical analyses and numerical simulations to better understand the factors driving …

A Brief Cognitive-Behavioral Intervention For Emotion Regulationand Aggression In Justice-Involved Adolescents: Preliminary Outcomes From A Non-Randomized Trial In Short-Term Juvenile Detention, Cassidy L. Tennity

Dissertations

Justice-involved youth are at risk for a variety of psychosocial difficulties, including anger, aggression, and emotion regulation challenges. Short-term juvenile detention settings offer a unique opportunity to provide psychological interventions to youth before they are returned to the community or sentenced to long-term placements. However, implementation barriers within these settings often hinder the delivery of evidence-based care. This study developed and tested a brief, two-session anger management intervention for adolescents detained in a short-term juvenile detention center in the Southeastern United States, drawing on evidence-based practices from prior work with justice-involved youth. The present study examined implementation outcomes (i.e., feasibility, …

The Impact Of Gender, Training Year, Medical Specialty And The Covid-19 Pandemic On Resident Wellness, Sara Beachy, Jessica Luzier, Chantel Weisenmuller, Kathleen Bors, Mary Ann Maurer, Amna Anees, Tiffany Lasky, Lisa Calderwood

Department of Family & Community Medicine Faculty Papers

INTRODUCTION: Data are limited as to which factors most strongly impact medical trainees' well-being, and few studies have assessed trainee's wellness at multiple time points during the COVID-19 pandemic. Additionally, much research has not taken an intersectional approach to understanding the ways in which different facets of a resident's identity impact their wellness. Thus, more person-centred, probability-driven statistical approaches that can produce distinct heterogenous groups of individuals are warranted to understand the way in which different identities are associated with resident wellness scores.

METHODS: A latent profile analysis was conducted using institutional data from resident surveys administered from 2019 to …

Optimal Hypergraph Connectivity With Cut Queries, Hang Liao

Dartmouth College Ph.D Dissertations

Finding connected components in undirected hypergraphs—hypergraph connectivity—is a fundamental problem in computer science. It can be framed as a special case of Symmetric Submodular Function Minimization (SSFM), where the objective is to determine if the non-trivial minimizer is zero. This thesis develops an optimal algorithm for hypergraph connectivity within the $\CUT$ query model, where an algorithm probes a subset of vertices to learn the weight of the hyperedges ``cut" by that partition.

Our approach is constructive, culminating in an optimal algorithm for the general problem by first developing the necessary tools for two foundational subproblems. The main contributions of this …

Exploring Intra- And Interpersonal Connection Through Mindful Moments At A Hispanic-Serving Institution, Yvette Angeline

Theses and Dissertations

The demands of contemporary education have contributed to high levels of stress, anxiety, and burnout among educators (Barton, 2019; Hidajat et al., 2023; Walker, 2022), uncovering a need for pedagogical approaches that prioritize holistic well-being and humanize the learning experience (Palmer, 2009; Salazar, 2013; Wong & Mishra, 2020). This qualitative case study investigates how moments of mindfulness, integrated as curricular practice within a teacher education program at a Hispanic-Serving Institution (HSI), nurtures pre-service teachers’ awareness of the self and world. This study explores aspects of mindfulness from its Eastern origins (Thich Nhat Hanh, 1975) and its Western secular applications (Jon …

Assessment Of Floral Resources From Three Tamaulipan Thornscrub Communities Of The Lower Rio Grande Valley, Gracie Makenna Granados

Theses and Dissertations

The Tamaulipan thornscrub is a transition zone between the neotropics and the temperate regions, harboring high biodiversity. This habitat supports a diverse insect community, including many pollinators of native and cultivated plants in Texas and northeastern Mexico. This ecosystem has been extensively degraded and lost; only 5% of its original cover remains north of the Rio Grande. Restoration of the Tamaulipan thornscrub has been ongoing for over three decades, but the important roles of pollinators in the success of such efforts and the functioning of the restored ecosystem have rarely been considered in restoration efforts. The purpose of this study …

Bilateral Leading Edges With Tubercle Modifications: An Experimental Study, Roberto Sanchez

Theses and Dissertations

The increasing demand for better performance and maneuverability from airfoils to sustain superior performance over a wide range of platforms, including vehicles, propellers, and wing designs, continues to grow. Researchers have drawn inspiration from nature, looking at birds of prey, dragonflies, and humpback whales for aerodynamic improvements. Among these, tubercle airfoils, inspired by the humpback whale’s flipper, have gained increasing interest. This experimental study presents the results of bilateral tubercle leading-edge airfoils based on a modified NACA 0018 design with a chord length of 1.82 inches, a span width of 7.5 inches, and an amplitude of 6.3c%. Five configurations were …

Development Of A Wearable Biosensor For Cortisol Detection In Human Sweat Utilizing Pani/Peo Polymer Blend And Nitrogen-Doped Graphene Quantum Dots, Cesar Sanchez

Theses and Dissertations

Since the development of the first biosensor in 1962, the evolution of commercially available wearable biosensors has been driven by an increasing consumer interest in real-time health tracking. However, challenges related to sensor sensitivity and cost have hindered progress. Deploying a novel approach, the biosensor integrates a blend of Polyaniline (PANi) and Polyethylene Oxide (PEO) along with Nitrogen-doped Graphene Quantum Dots (N-GQDs) to enhance sensitivity and electroactivity, crucial for cortisol detection. To optimize performance, three distinct synthesis methods for N-GQDs; hydrothermal, microwave, and hot plate synthesis were compared employing a bottom-up approach utilizing citric acid (CA) as the primary reactant …

The Impact Of Positive Psychology Use Upon Gls Outcomes For People With Chronic Pain Syndromes, Timothy Michael Curtis

Theses and Dissertations

The essence of being human is realized by our discovery of meaningful purpose throughout each important stage of our lives. If we perceive that we lack this meaningful purpose in our lives, it could become a distressful source of suffering to our sense of well-being emotionally, cognitively and perhaps, spiritually. A strong indicator of well-being comes from a good evaluation we ascertain as to how well we believe outcomes in our lives are consistent with our goals and values, which is also known as satisfaction with life. Research findings indicate that the more someone experiences repeated negative life events, the …

Arginine Vasotocin And Corticosterone In Nesting And Non-Nesting Loggerhead Sea Turtles In X’Cacel-X’Cacelito Sanctuary, Krista Blair Reed

Theses and Dissertations

Sea turtles exhibit distinct behaviors before and after egg laying, however, not all individuals successfully oviposit. Some females display disruptions in their typical nesting behavior, returning to the water without laying eggs, which is referred to as a non-nesting emergence (NNE). NNEs can be due to external disturbances on the beach, but why they occur is often unclear. Arginine vasotocin (AVT) is a neuropeptide involved in various physiological processes, including stimulating smooth muscle contraction of the oviduct to facilitate egg deposition in nesting sea turtles. On the other hand, corticosterone is the primary stress hormone in sea turtles and is …

Exploring Transcriptional Stress Responses In Sorghum And Evolutionary Insights Into Yth Domain Proteins Across Plants, Shikha Bharti

Theses and Dissertations

Understanding how plants respond to abiotic stress is crucial for enhancing crop resilience in the face of climate change. This thesis explores plant stress adaptation mechanisms through two interlinked studies that address different aspects of gene regulation. The first study investigates the transcriptional dynamics of Sorghum bicolor under drought and heat stress at 1-hour and 6-hour time points. By employing 3'-end RNA sequencing, this study provides a comprehensive temporal view of gene expression changes, revealing stress-responsive transcription factors, hormone-associated pathways, and chromatin regulators. The comparative analysis highlights both common and distinct molecular responses between drought and heat stress, providing insights …

Biological, Histological, And Immunohistochemical Responses To Heat Stress In Common Goldfish, Carassius Auratus, Ngozichukwu Francis Ngozichukwu

Theses and Dissertations

Global climate change has led to rising temperatures, posing significant challenges to aquatic ecosystems and the health of ectothermic species such as goldfish (Carassius auratus) This thesis investigated the effects of heat stress on growth performance, histological alterations, and molecular stress responses in the gill and kidney tissues of goldfish Fish were exposed to control (24°C), medium (28°C), and high (32°C) temperatures for two weeks Growth parameters, including instantaneous growth rate (IGR), absolute growth rate (AGR), body value gain (BVG), and condition factor, were evaluated alongside histological and immunohistochemical markers of stress Results revealed that high temperature significantly reduced AGR …

Analyzing How Taste And Nutrition Impact Learning And Memory In Honey Bees Through Olfactory Associative Conditioning, Alessandra Elizabeth Perry

Theses and Dissertations

Honey bees detect sweet nectar, but not all sweet-tasting sugars provide nutrition. Unlike fruit flies, which can learn to avoid non-nutritious sweet foods, bees may lack this ability. To test if bees form caloric frustration memory (CFM), we conditioned them using odors paired with L-glucose (sweet but non-nutritious) or D-glucose (sweet and nutritious). Bees rewarded with L-glucose showed lower learning and long-term memory compared to D-glucose. Bees cannot differentiate between an odor associated with rewards containing malaise-causing compounds and an odor associated with a reward alone during differential conditioning. Therefore, we used differential conditioning to determine if lower acquisition in …

Transcriptomic Study On The Effect Of Salt Stress In Sorghum Bicolor Seedlings, Joshwa A. Gandy

Theses and Dissertations

The global impact and reliance on cereal crops, and sorghum in particular, is paramount for worldwide food security and for the livelihood of hundreds of millions of people. The detrimental effects of salt stress on crop plants and the huge economic losses it causes by affecting agricultural output worldwide is a devastating issue with huge impacts, which have prompted research to combat salinity in numerous ways. Understanding how cereals respond to the negative effects of salt stress is crucial for enhancing their salt tolerance. In this study of sorghum, we used a 3’-end RNA sequencing approach to elucidate major salt …

Interdigitated Gear-Shaped Screen-Printed Electrode Using G-Pani Ink For Sensitive Electrochemical Detection Of Stress Related Hormones Dopamine And Cortisol, Pritu Parna Sarkar

Theses and Dissertations

This research introduces a novel interdigitated gear-shaped graphene-based electrochemical biosensor for the simultaneous detection of dopamine (DA) and cortisol. Modified with graphene–polyaniline (G–PANI) conductive ink, the sensor increased active surface area—94.52% and 57% higher than Metrohm DropSens 110 and circular printed sensors, respectively. Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR) Spectroscopy and X-ray diffraction (XRD) were employed for physicochemical characterization. The sensor detects dopamine and cortisol over a linear range of 0.1–100 µM, with limits of detection of 0.043 µM (R² = 0.98) and 0.0577 µg/mL (R² = 0.976), respectively, in phosphate-buffered saline (PBS) using redox probe. Electrochemical performance …

Opa1-Mediated Mitochondrial Dynamics In Myogenic Differentiation Of C2c12 Myoblasts, Daniel Eloy Bazan

Theses and Dissertations

Mitochondria play a pivotal role in metabolic energy production, cellular signaling, and apoptosis. To carry out these key cellular roles, mitochondria dynamically balance their organization through opposing including fusion and fission processes. The balance between mitochondrial fusion and fission is regulated by key proteins such as OPA1 (optic atrophy 1) and OMA1 (overlapping activity with metalloprotease). More recently, mitochondria have been increasingly revealed to play key roles in cellular differentiation, where metabolic demands are reprogrammed. This study aims to elucidate the specific roles of OPA1 and OMA1 in the differentiation of C2C12 myoblast cells into myotubes.

Our data demonstrates that …

Machine Learning Applications For Evaporation Predictions From Small Reservoirs: Potential Water Savings For Lower Rio Grande Valley, Texas, Syed Muhammad Fahad Abdullah

Theses and Dissertations

Local-scale reservoirs are important to regional water balance, but these are often overlooked. This study presents a robust machine learning (ML) approach leveraging reanalysis datasets to estimate daily evaporation for local-scale reservoirs in semi-arid South Texas. Selected models were trained with daily lake evaporation model (DLEM) estimates and used climatic and reservoir-specific properties as feature input variables. The multi-reservoirs training approach ensured applicable model generalization. Results show promising predictive performance with R² values ranging from 0.55–0.67 (testing) and 0.64–0.78 (validation), NSE values ranged from 0.54 0.67 (testing) and 0.64–0.78 (validation), and RMSE values ranged between 1.52–1.80 mm/day (testing) and 1.22–1.58 …

Phenotypic Plasticity In Response To Abiotic Factors In Silverleaf Nightshade (Solanum Elaeagnifolium), And The Effectiveness Of North American Lacebug (Gargaphia Arizonica Drake And Calvo) As A Biocontrol, Marco Andre Silguero

Theses and Dissertations

Silverleaf nightshade (Solanum elaeagnifolium) is a widespread invasive weed in South Texas and other arid regions, where it reduces crop productivity, competes with native vegetation, and resists conventional control methods. Its ability to thrive across different environments is linked to phenotypic plasticity, allowing it to adjust growth and physiology in response to light and water availability. In this thesis, I examine the role of plasticity in S. elaeagnifolium under field conditions and evaluate the potential of the North American lacebug (Gargaphia arizonica) as a biological control agent. Field observations were used to measure plant traits such as height, stem thickness, …

Investigating The Multiple Enzyme Complex In The Carotenoid Biosynthesis Pathway, Mercy Mormor

Electronic Theses and Dissertations

In nature, plant carotenoids are 40-carbon isoprenoids with polyene chains that may have double bonds. They are vital components of all photosynthetic organisms, playing several roles in the plant cell, including photosynthesis, pigmentation, phytohormone synthesis, signaling and photoprotection. In this study, we investigated protein-protein interactions among key enzymes involved in the carotenoid biosynthesis pathway using the Bimolecular Fluorescent Complementation (BiFC) assay. Our results revealed several interactions that suggest a coordinated regulation of enzymatic activity within this pathway, highlighting potential regulatory complexes that influence carotenoid production. The study also highlights the pivotal role of Zeta-Carotene Isomerase (Z-ISO) as a key regulatory …

Gut Passage Time In Small Mammals: Influences Of Small Mammal Traits, Digesta Phase, And Particle Morphology, Noah G. Hale

Electronic Theses and Dissertations

Dispersal is crucial in maintaining healthy ecosystems, but little is known about mechanisms influencing fungal spore dispersal by animals. We aimed to develop a more mechanistic understanding of the influence of fungal spore traits and small mammal traits on gut retention time. We fed three species of small mammals (shrew, mouse, vole), which differ in gut morphologies, digestive strategies, and activity a combination of markers including: liquid digesta phase (CrEDTA), small particle (fungal spores with diverse morphologies), and large particles (glitter and beads, representing plant cells and seeds, respectively). We found that hindgut fermenters selectively retained the liquid markers, spores …

Cattle Regenerative Grazing Effects On Bat Activity In Southern Appalachia, Seth Morelock

Electronic Theses and Dissertations

Livestock farming accounts for about 70% of agricultural activity, with continuous grazing as the dominant conventional practice. However, this method often degrades biodiversity and ecosystem health through overgrazing. Regenerative grazing practices offer a potential ecologically sustainable approach to cattle management. Insectivorous bats are known to use agroecosystems for foraging and roosting habitats, offering an opportunity to assess their response to regenerative grazing. Using passive acoustic monitors, we evaluated the response of bats to cattle within regenerative grazed farms in East Tennessee, USA. Overall bat activity declined when cattle entered and exited paddocks, except for the tri-colored bat (Perimyotis subflavus …

Sarcoplasmic Reticulum-Mitochondria Microdomains: Hugging And Kissing In The Heart, Bong Sook Jhun, Jin O-Uchi, Brian Rhee, Ameneh Ahrari, Nathan Demichaelis, Kye-Im Jeon, David M. Booth, Shey-Shing Sheu

Center for Translational Medicine Faculty Papers

Endoplasmic reticulum (ER)-mitochondrial (ER-Mito) interface, termed mitochondrial-ER contacts (MERCs), plays significant roles in the maintenance of bioenergetics and basal cell functions via the exchange of lipids, Ca 2 þ , and reactive oxygen species (ROS) in various cell types/tissues. Genetic deletion of mitofusin 2 (Mfn2), one of the key components of ER-Mito tethering, in cardiomy- ocytes (CMs) in vivo revealed the importance of the microdomains between mitochondria and sarcoplasmic reticulum (SR), a dif- ferentiated form of ER in muscle cells, for maintaining normal mitochondrial Ca 2 þ (mtCa 2 þ ) handling and bioenergetics in the adult heart. However, key …

Rapid Dissection And Dissociation Of The Mouse Olfactory Epithelium For Single-Nucleus Suspensions, Benjamin D W Belfort, Anthony M Insalaco, Claude C Chew, Johnathan D Jia, Julia Younis, Benjamin R Arenkiel

Duncan NRI Faculty and Staff Publications

The murine olfactory epithelium is the initial entry point of the olfactory system, housing various cell types that include olfactory sensory neurons, their regenerating progenitors, and support cells. Olfactory sensory neurons transduce chemical odorants into neural signals, yet the mechanisms underlying how these cells develop and turnover, create synapses with the olfactory bulb, and regulate their odorant receptors remain areas of intense study. Located on the dorsal aspect of the nasal cavity, the olfactory epithelium adheres to intricate bony structures known as turbinates. This anatomy poses unique challenges for its extraction and dissociation, especially in the context of preparing viable …