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2025

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Screen Time And Objectively Measured Sleep Of U.S. College Students: A Brief Report, Donya Sedaghat, Eunkyung Lee, Jeanette Garcia, Keith Brazendale Aug 2025

Screen Time And Objectively Measured Sleep Of U.S. College Students: A Brief Report, Donya Sedaghat, Eunkyung Lee, Jeanette Garcia, Keith Brazendale

International Journal of Exercise Science

International Journal of Exercise Science 18(4): 747-756, 2025. The objective was to investigate screen time (ST) and objectively measured sleep quality in college students. Participants were undergraduate students attending a large metropolitan public university in the southeastern U.S. Participants wore accelerometers on their wrist for 24hr/day for 14 days to capture sleep outcomes, and completed a survey reporting ST duration. Analyses explored weight-status, race/ethnicity, and sleep patterns (presence of daytime sleep) differences among students. Participants (n=29, 86% female, ~21.5 years old, 38% White, 35% Overweight/Obese) slept for an average of 433.8 minutes/night, reported 419.1 minutes of daily ST, and 112 …


Classify Cancers Types Using Mirnas Profiles And Machine Learning, Wafaa Khazaal Shams Aug 2025

Classify Cancers Types Using Mirnas Profiles And Machine Learning, Wafaa Khazaal Shams

AUIQ Technical Engineering Science

Cancer classification is important for early diagnosis and effective treatment. This study proposes a hybrid framework that combines Fisher Discriminant analysis (FD) with Linear Support Vector Machine (LSVM) and Long Short-Term Memory (LSTM) networks. MicroRNAs (miRNAs) expression profiles for three types of cancer are used to evaluate the model. These are: lung- adenocarcinoma, ovary and pancreas cancers. The classification process is done using 5-fold cross valuation. Results show significant performance for LSTM compared to LSVM with and without features reduction. However, using FD analysis results in high accuracy reached 0.97and 0.96 for LSTM and LSVM respectively, using 50 miRNAs. The …


Architectural Optimization Of Façade Design Using Nano-Ceramic Films For Green Buildings In Baghdad’S Hot Climate, Lina M. Shaker, Wan Nor Roslam Wan Isahak, Ahmed Salih Mahdi Aug 2025

Architectural Optimization Of Façade Design Using Nano-Ceramic Films For Green Buildings In Baghdad’S Hot Climate, Lina M. Shaker, Wan Nor Roslam Wan Isahak, Ahmed Salih Mahdi

AUIQ Technical Engineering Science

This study aims to optimize the architectural integration and energy performance of nano-ceramic window films in a three-story Baghdad commercial building having an overall area of 2,000 m2. In a 40% window-to-wall ratio (WWR) and climate-responsive orientation, films were selected for solar exposure; north façade overhanging high-transmittance movies, east and west vertical fins on medium-transmittance movies, and south horizontal louvers on low-transmittance movies. Architectural strategies for daylighting, comfort, and façade appearance with low solar heat gain (SHG) were considered. MATLAB 2022 simulations evaluated energy savings, daylight transmission, and solar rejection. The most effective film (either film, HD-IR05100) achieved …


Illinois Model Programs Of Study Guide: Health Sciences, Education Systems Center Aug 2025

Illinois Model Programs Of Study Guide: Health Sciences, Education Systems Center

Reports, Analyses, and other Publications

First published in October 2020. Significantly revised in September 2024 to incorporate updated labor market information. An update in April 2025 included minor corrections. In August 2025, two new sections were added: key terminology and the relationship between concepts.


High School Teachers’ Experiences Using Data-Driven Decision Making To Implement Supplemental Accelerated Instruction, Stephen Courtney Smith, Sr. Aug 2025

High School Teachers’ Experiences Using Data-Driven Decision Making To Implement Supplemental Accelerated Instruction, Stephen Courtney Smith, Sr.

Walden Dissertations and Doctoral Studies

The problem that was addressed in this study was that high school teachers in a Texas school district struggle to use data-driven decision making (DDDM) to meet the requirements of Texas HB 1416, which mandates specific interventions to address instructional loss. Grounded in the theory of DDDM, by Mandinach et al., the purpose of this basic qualitative study was to explore high school teachers’ experiences using DDDM to implement supplemental accelerated instruction (SAI) for students who failed to demonstrate proficiency on state exams. Data were collected using semistructured interviews with 12 high school teachers from a Texas school district. Using …


Illinois Model Programs Of Study Guide: Agriculture, Food, And Natural Resources, Education Systems Center Aug 2025

Illinois Model Programs Of Study Guide: Agriculture, Food, And Natural Resources, Education Systems Center

Reports, Analyses, and other Publications

First published in August 2021. Significantly revised in September 2024 to incorporate updated labor market information. An update in April 2025 included minor corrections. In August 2025, two new sections were added: key terminology and the relationship between concepts.


Developing A Course-Based Undergraduate Research Experience For Chem 122, Melanie J. Harvey Phd Aug 2025

Developing A Course-Based Undergraduate Research Experience For Chem 122, Melanie J. Harvey Phd

Sabbatical Projects

Sabbatical presentation from a 2022 Sabbatical


Male Caregiving Experience Alters Hippocampal Neuroplasticity And Transcription Independent Of Reproduction In A Biparental Species., Maria E Colt, Priyanka Agarwal, Erica R Glasper, Heidi Fisher Aug 2025

Male Caregiving Experience Alters Hippocampal Neuroplasticity And Transcription Independent Of Reproduction In A Biparental Species., Maria E Colt, Priyanka Agarwal, Erica R Glasper, Heidi Fisher

Faculty Research 2025

In mammals, measurable changes in brain and behavior accompany the transition to parenthood. In the biparental California mouse, Peromyscus californicus, fathers experience enhanced neuroplasticity, including increased hippocampal dendritic spine density, reduced anxiety, and improved memory. Here, we first investigate whether siring offspring or pup interaction drives structural neuroplasticity in fathers and find that hippocampal spine density is positively associated with caregiving experience, even in the absence of reproduction. Next, we evaluate the transcriptional response to caregiving in the hippocampus of these males and identify 158 differentially expressed genes between fathers and non-fathers, many of which are associated with neurogenesis, neuronal …


Expanding Functionalities Of Immune Receptors Through Simultaneous Transfer Of Sensor And Helper Nlrs, Dongjiao Wang, Qibin Wu, Daowen Wang, Youxiong Que, Zheng Qing Fu Aug 2025

Expanding Functionalities Of Immune Receptors Through Simultaneous Transfer Of Sensor And Helper Nlrs, Dongjiao Wang, Qibin Wu, Daowen Wang, Youxiong Que, Zheng Qing Fu

Faculty Publications

No abstract provided.


Illinois Model Programs Of Study Guide: Culinary And Hospitality, Education Systems Center Aug 2025

Illinois Model Programs Of Study Guide: Culinary And Hospitality, Education Systems Center

Reports, Analyses, and other Publications

First published in October 2022. Significantly revised in April 2025 to incorporate updated labor market information. In August 2025, two new sections were added: key terminology and the relationship between concepts.


The Role Of The Poly-N Repeat In The Plasmodium Falciparum Histone Acetyltransferase Protein, Pfgcn5, Kely Johana Rubiano Cardona Aug 2025

The Role Of The Poly-N Repeat In The Plasmodium Falciparum Histone Acetyltransferase Protein, Pfgcn5, Kely Johana Rubiano Cardona

Arts & Sciences Graduate Student Theses and Dissertations

P. falciparum possesses the most AT-rich genome sequenced to date, with long AAT repeats coding for asparagines (N) in intragenic regions. As a result, nearly a quarter of its proteome is composed of proteins with poly-N repeats. Since the discovery of its genome, the function of these repeats has remained as one of the biggest mysteries in the biology of this parasite. Furthermore, it has long been known that proteins with N-rich domains aggregate easily, particularly during heat stress (HS). Given that the hallmark of this disease is recurrent febrile episodes, it is not well understood how the parasite survives …


Role Of Perceived Social Support And Knowledge Of Postpartum Depression Among Women In Anambra State, Eastern Nigeria, Raphael Oluwasina Babatola Folorunsho Aug 2025

Role Of Perceived Social Support And Knowledge Of Postpartum Depression Among Women In Anambra State, Eastern Nigeria, Raphael Oluwasina Babatola Folorunsho

Walden Dissertations and Doctoral Studies

Postpartum depression (PPD) is an increasingly pressing public health issue, particularly in low-resource settings like Nigeria, where maternal mental health tends to receive limited attention. This study explored the relationship between maternal awareness of PPD, perceived social support, and prevalence of depressive symptoms among postpartum women in the Nnewi North Local Government Area (LGA) of Anambra State. Grounded in the social support theory, health belief model, and socioecological model, the study used a descriptive cross-sectional research design. A total of 406 postpartum women attending primary healthcare centers were surveyed using validated instruments, including the PHQ-9 and MSPSS. Statistical analyses included …


Bclaf1 Is A Novel Regulator Of Hematopoietic Stem Cells, Stephanie Crowley Aug 2025

Bclaf1 Is A Novel Regulator Of Hematopoietic Stem Cells, Stephanie Crowley

Arts & Sciences Graduate Student Theses and Dissertations

Hematopoietic stem cells (HSC) maintain both a self-renewal capacity to persist throughout life and an ability to differentiate into mature cell lineages. HSC self-renewal programs are often ectopically activated in leukemia cells to sustain developmental arrest and neoplastic proliferation. Recently, the transcriptional regulator Bclaf1 (Bcl2 associated transcription factor 1) was shown to support proliferation and block differentiation of AML blasts. Bclaf1 is ubiquitously expressed in hematopoietic cells but its function in normal hematopoiesis has not been elucidated. Our data demonstrate that BCLAF1 promotes HSC development and function. To study the role of BCLAF1 in normal HSCs, we used murine models …


Determining The Origin Of An Isolated Great Lakes Plant Species, Agoseris Glauca (Asteraceae), Seth Paul Hiers Aug 2025

Determining The Origin Of An Isolated Great Lakes Plant Species, Agoseris Glauca (Asteraceae), Seth Paul Hiers

Masters Theses

Small, isolated populations often have low genetic diversity and low potential for adaptation in response to environmental changes. Alternatively, isolated communities are often more likely to display local adaptations and over time speciate. The Great Lakes Region is home to over 50 vascular plant species that are disjunct from the species’ core populations. Agoseris glauca (Asteraceae) is a Great Lakes disjunct and Michigan state threatened species geographically isolated from its core range by 900 km. This study uses Genotyping by Sequencing to assess the population genetics of A. glauca for two Michigan disjunct populations and five western populations in the …


Gastrointestinal Fistulas-What Gastroenterologists Need To Know In 2025, Monjur Ahmed Aug 2025

Gastrointestinal Fistulas-What Gastroenterologists Need To Know In 2025, Monjur Ahmed

Division of Gastroenterology and Hepatology Faculty Papers

Gastrointestinal fistulas are increasingly being encountered in our clinical practice because of the increased burden of Crohn's disease, bariatric surgeries, interventional endoscopic procedures, nonsurgical trauma, and war and disaster zones worldwide. Presentation depends on the location and specific type of the fistula. Symptomatic ones can have a tremendous impact on social life and can cause dehydration, electrolyte imbalance, malnutrition, increased morbidity, and mortality. Different imaging studies and endoscopic procedures are done to establish the diagnosis. Treatment modalities to close the fistula depend on the underlying disease and the type of fistula. They include conservative treatment, medical therapy, endoscopic interventions, and …


Butte Priority Soils Operable Unit (Bpsou) Draft Final Reclamation Improvement Sites Field Sampling And Investigation Plan (Fsp): Bres No. 18 – Walkerville Ballfield, Mike Mcanulty Aug 2025

Butte Priority Soils Operable Unit (Bpsou) Draft Final Reclamation Improvement Sites Field Sampling And Investigation Plan (Fsp): Bres No. 18 – Walkerville Ballfield, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.


Implementation Of A Forensic Ancestry Inference Indel Panel, Jacqueline Molina Aug 2025

Implementation Of A Forensic Ancestry Inference Indel Panel, Jacqueline Molina

Student Theses

Ancestry-informative markers (AIMs) are genetic variants that exhibit significant frequency differences between populations, making them valuable tools for inferring biogeographical ancestry in forensic investigations. Insertion/deletion polymorphisms (INDELs) offer particular advantages for degraded forensic samples due to their short amplicon sizes and binary allelic nature. As forensic laboratories adopt newer instrumentation, validation of established assays becomes critical to ensure analytical continuity and reliability. This study evaluated the comparative performance of the SeqStudio™ Genetic Analyzer in detecting a 46 AIMs INDEL multiplex panel, previously established with the ABI 3130 Genetic Analyzer. Four commercial blood samples were analyzed using both platforms to assess …


Expanding Functionalities Of Immune Receptors Through Simultaneous Transfer Of Sensor And Helper Nlrs, Dongjiao Wang, Qibin Wu, Daowen Wang, Youxiong Que, Zheng Qing Fu Aug 2025

Expanding Functionalities Of Immune Receptors Through Simultaneous Transfer Of Sensor And Helper Nlrs, Dongjiao Wang, Qibin Wu, Daowen Wang, Youxiong Que, Zheng Qing Fu

Faculty Publications

No abstract provided.


The Impact Of Family Violence On Bullying Behaviour Among Secondary School Students In Ilorin Metropolis, Oyedotun Dolapo Ilori, Patrick O. Akerele, Ifeoluwa Blessing Michael Aug 2025

The Impact Of Family Violence On Bullying Behaviour Among Secondary School Students In Ilorin Metropolis, Oyedotun Dolapo Ilori, Patrick O. Akerele, Ifeoluwa Blessing Michael

Buletin Konseling Inovatif

Family violence remains a significant social issue with far-reaching consequences for adolescents, particularly in shaping their behavioural tendencies, including bullying. This study examines the impact of family violence on bullying behaviour among secondary school students in Ilorin Metropolis, which comprises three Local Government Areas (Ilorin South, Ilorin West, and Ilorin East). The study adopts a descriptive survey research design to assess the extent to which exposure to family violence influences students' bullying behaviours, while also considering the role of peer influence. A multi-stage sampling technique was employed to select 417 students from both public and private secondary schools in Ilorin …


Cardiometabolic Outcomes In The Obese African American Population With Rheumatoid Arthritis, Cecilia Jillo Walan Aug 2025

Cardiometabolic Outcomes In The Obese African American Population With Rheumatoid Arthritis, Cecilia Jillo Walan

Walden Dissertations and Doctoral Studies

Rheumatoid arthritis (RA) disproportionately impacts African American adults, particularly those with obesity, due to overlapping cardiometabolic risks and health care disparities. The intersection of RA, obesity, and chronic conditions such as cardiovascular disease (CVD) and hypertension underscores the need for targeted public health interventions. This study investigated the association between RA and cardiometabolic outcomes among African American adults with obesity, aiming to identify significant predictors within this high-risk population. This quantitative study employed a cross-sectional design to analyze data from the 1999–2018 National Health and Nutrition Examination Survey undergirded by the social ecological change framework with N=23644 African American adults …


Re: Butte Priority Soils Operable Unit (Bpsou) Final Insufficiently Reclaimed Sites Field Sampling And Investigation Plan (Fsp) For Ur-19 – Butte, Anaconda & Pacific Railway From Montana Street To South Arizona Avenue – Montana Street Adjacent Parcel, Mike Mcanulty Aug 2025

Re: Butte Priority Soils Operable Unit (Bpsou) Final Insufficiently Reclaimed Sites Field Sampling And Investigation Plan (Fsp) For Ur-19 – Butte, Anaconda & Pacific Railway From Montana Street To South Arizona Avenue – Montana Street Adjacent Parcel, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.


2025 Fall Convocation Program, Morehead State University. Office Of Communications & Marketing. Aug 2025

2025 Fall Convocation Program, Morehead State University. Office Of Communications & Marketing.

Communications and Marketing Publications Archive

The 2025 Morehead State University Convocation Program from August 13, 2025.


Ribosome Deficiency Induces Salmonella Filamentation Within Host Cells, Zhihui Lyu, Cierra Wilson, Kalyn Weiss, Spencer Lewis, Kurt Fredrick, William Margolin, Jiqiang Ling Aug 2025

Ribosome Deficiency Induces Salmonella Filamentation Within Host Cells, Zhihui Lyu, Cierra Wilson, Kalyn Weiss, Spencer Lewis, Kurt Fredrick, William Margolin, Jiqiang Ling

Faculty, Staff and Student Publications

The ribosome is the central hub for protein synthesis and is heavily targeted by antibiotics. Ribosomal mutations, antibiotic treatment, and nutrient starvation can alter translational efficiency and lead to stressed cells. Ribosome deficiency plays a critical role in stress responses and disease progression; yet, how it affects bacteria-host interactions remains poorly understood. In this study, we show that a ribosome-deficient strain exhibits a surprising morphological change from rod shape to filamentous in Salmonella cells growing inside host macrophages. Such filamentation depends on an acidic condition within macrophages and in a defined medium mimicking macrophage conditions. Further genetic analyses revealed that …


Csf-Exosomal Mirnas And Delayed Cerebral Ischemia: Insights Into Pathophysiology But No Definitive Biomarkers, Chathathayil M Shafeeque, Devin W Mcbride, Yuanqing Yan, Hussein A Zeineddine, John P Hagen, H Alex Choi, Jude P Savarraj, Ari Dienel, Spiros L Blackburn, Peeyush Kumar Thankamani Aug 2025

Csf-Exosomal Mirnas And Delayed Cerebral Ischemia: Insights Into Pathophysiology But No Definitive Biomarkers, Chathathayil M Shafeeque, Devin W Mcbride, Yuanqing Yan, Hussein A Zeineddine, John P Hagen, H Alex Choi, Jude P Savarraj, Ari Dienel, Spiros L Blackburn, Peeyush Kumar Thankamani

Faculty, Staff and Student Publications

Background: Aneurysmal subarachnoid hemorrhage (aSAH) is notoriously known for its high mortality and morbidity. Approximately one-third of the patients who survive aneurysm rupture are reported to develop delayed cerebral ischemia (DCI), which contributes to a poor clinical outcome. Currently, there are no biomarkers for identifying which aSAH patients are at risk of developing DCI. We aimed to determine the feasibility of cerebrospinal fluid (CSF) exosomal microRNAs (miRNAs) for predicting DCI post-aSAH.

Methods: aSAH patients were prospectively enrolled, and CSF samples were collected at two time points (< 24 h and 72 h post-aSAH) from individuals undergoing external ventricular drainage. Exosomal miRNAs were isolated from the CSF for analysis. In the initial group of patients (discovery cohort), an exploratory analysis was conducted using a CSF panel containing 84 miRNAs, assessed by quantitative real-time PCR (RT-qPCR). Based on this analysis, 27 miRNAs were selected for further evaluation in a second group of patients (validation cohort). Among these, 10 miRNAs had previously been reported in SAH-related CSF studies, supporting their relevance for continued investigation.

Results: In this study, RT-qPCR analysis of 84 miRNAs in CSF samples from …


Reduced Computed Tomography Scan Speed Improves Alignment Errors For Patients Undergoing Thoracic Stereotactic Body Radiation Therapy, Ramaswamy Sadagopan, Rachael M Martin-Paulpeter, Christopher R Peeler, Xiaochun Wang, Paige Nitsch, Julianne M Pollard-Larkin Aug 2025

Reduced Computed Tomography Scan Speed Improves Alignment Errors For Patients Undergoing Thoracic Stereotactic Body Radiation Therapy, Ramaswamy Sadagopan, Rachael M Martin-Paulpeter, Christopher R Peeler, Xiaochun Wang, Paige Nitsch, Julianne M Pollard-Larkin

Faculty, Staff and Student Publications

Objectives: We investigated the performance of a slow computed tomography (CT) protocol to reduce alignment errors arising from motion when using CT-on-rail (CTOR) for image guidance for patients receiving thoracic stereotactic body radiation therapy (SBRT).

Methods: A Quasar lung phantom with a moving tumor was programmed with three breathing rates and three motion amplitudes. MIP and average 4DCT images were used for contouring and alignment, respectively. Ten CTOR images were obtained for each of the breathing rates and amplitudes, under both CT protocols. We used in-house CAT software for image guidance, centering the tumor in the lung window …


Identification Of Alkynyl Nicotinamide Hsn748 As A Ret Solvent-Front Mutant Inhibitor With Intracranial Efficacy, Ujjwol Khatri, Neetu Dayal, Kofi B Owusu, Mandeep Kaur Hunjan, Shriya Pandey, Haley Anne Harper, Carli Mcmahan, Bennett D Elzey, Tao Shen, Xueqing Hu, Kurt W Evans, Ahmed El-Sheikh, Seong Jun Jo, Frederick W Holtsberg, M Javad Aman, Funda Meric-Bernstam, Sukyung Woo, Herman O Sintim, Jie Wu Aug 2025

Identification Of Alkynyl Nicotinamide Hsn748 As A Ret Solvent-Front Mutant Inhibitor With Intracranial Efficacy, Ujjwol Khatri, Neetu Dayal, Kofi B Owusu, Mandeep Kaur Hunjan, Shriya Pandey, Haley Anne Harper, Carli Mcmahan, Bennett D Elzey, Tao Shen, Xueqing Hu, Kurt W Evans, Ahmed El-Sheikh, Seong Jun Jo, Frederick W Holtsberg, M Javad Aman, Funda Meric-Bernstam, Sukyung Woo, Herman O Sintim, Jie Wu

Faculty, Staff and Student Publications

RET solvent-front G810C/R/S mutations confer resistance to the currently approved RET protein tyrosine kinase inhibitors (TKIs) selpercatinib and pralsetinib. Moreover, RET fusion-positive lung adenocarcinoma frequently metastasizes to the brain. To address these challenges, it is imperative to develop a RET TKI that is effective against solvent-front mutations and exhibits intracranial activity. We synthesized alkynyl nicotinamide-based RET TKIs and tested their efficacy in cell cultures in inhibiting selpercatinib/pralsetinib-resistant RET solvent-front mutants G810C/R/S found in cancer patients, and in BaF3/KIF5B-RET(G810C) cell-derived subcutaneous and intracranial tumors in vivo. We also evaluated alkynyl nicotinamide RET TKIs in KIF5B-RET-induced lung tumors in immune competent …


The Effects Of Moderate-Intensity Physical Exercise And Yoga Interventions On Stress In Hispanic College Students: A Pilot Study, Hongxing Lu, Florentino Saenz, Preethi Raju, Ednia Gutierrez, Sue Anne Chew, Saraswathy Nair Aug 2025

The Effects Of Moderate-Intensity Physical Exercise And Yoga Interventions On Stress In Hispanic College Students: A Pilot Study, Hongxing Lu, Florentino Saenz, Preethi Raju, Ednia Gutierrez, Sue Anne Chew, Saraswathy Nair

Health & Biomedical Sciences Faculty Publications

Background: Hispanic college students face high stress, which may increase their risk for chronic stress-related health issues. Effective and accessible stress management strategies for this population remain limited. Objective: This pilot study filled a gap by studying the effects of psychological stress on diet and physical activity habits and evaluating the impact of moderate-intensity physical exercise (MIPE) and yoga interventions on chronic psychological stress and acute stress responses among Hispanic college students. Methods: A total of 18 Hispanic college students participated in a 6-week intervention consisting of either MIPE or yoga, conducted twice weekly. Anthropometric measurements and lifestyle data were …


Exploring Knowledge, Attitudes, And Practices Of Healthcare Providers In Addressing The Care Needs Of Individuals Who Identify As Lgbtqia+ : A Mixed-Method Study, Seda Karakaya Çataldaş, J. Anneke Rummens, Erin Ziegler, Fahriye Olfaz, Esra Ummak, C. Hürrem Ayhan Aug 2025

Exploring Knowledge, Attitudes, And Practices Of Healthcare Providers In Addressing The Care Needs Of Individuals Who Identify As Lgbtqia+ : A Mixed-Method Study, Seda Karakaya Çataldaş, J. Anneke Rummens, Erin Ziegler, Fahriye Olfaz, Esra Ummak, C. Hürrem Ayhan

Sigma Foundation for Nursing Research Grant Reports

This mixed-methodological study explores the knowledge, attitudes, and practices of healthcare providers (HCPs) and also gathers and analyzes their narrative experiences addressing the care needs of individuals who self-identify as LGBTQIA+. A s ociodemographic form, Knowledge and Practices to Address the Care Needs of LGBTQIA+ Individual Survey, and the Implicit Association T est ( Sexuality IAT and Transpictures IAT) were used as to collect quantitative data from 278 nurses and physicians. S emi-structured in-depth interviews were then held with 15 of these study participants.Quantitative findings revealed slight implicit attitudes favoring heterosexual and cisgender individuals, with education level and personal connections …


Advanced Specificity And Sensitivity Studies Relative To A Research Use Only Transcription-Mediated Amplification-Based Assay For Treponema Pallidum Rna Detection, Trinity Krueger, Grace Kadonsky, Elizabeth Thelen, Amanda Zapp, Josephine Moore, Ahmet Muslu, Allan Pillay, Irene A Stafford, Erik Munson Aug 2025

Advanced Specificity And Sensitivity Studies Relative To A Research Use Only Transcription-Mediated Amplification-Based Assay For Treponema Pallidum Rna Detection, Trinity Krueger, Grace Kadonsky, Elizabeth Thelen, Amanda Zapp, Josephine Moore, Ahmet Muslu, Allan Pillay, Irene A Stafford, Erik Munson

Faculty, Staff and Student Publications

Preliminary experimentation has suggested that a research-use-only real-time transcription-mediated amplification assay for Treponema pallidum (RUO T. pallidum TMA) yields instances of T. pallidum nucleic acid detection that coincide with non-treponemal serology in men who have sex with men (MSM) at increased risk for sexually transmitted infection. To further characterize the specificity of RUO T. pallidum TMA testing, 3,586 rectal swab specimens reported as “not detected” by the assay generated a mean endpoint FAM fluorescence of 637.5 units. Introduction of Treponema denticola, Treponema phagedenis, and Treponema refringens nucleic acid into matrices generated mean endpoint FAM fluorescence ranging from 620.7 …


An Exciting Future For Microbial Molecular Biology And Physiology, Andrew A Bridges, Leah Guthrie, Mckenzie Lehman, Elizabeth H Kellogg, Samantha Wellington Miranda, Andrew Pountain, Anthony L Shiver, Andrew Varble, Molecular Biology And Physiology Community Of The Council On Microbial Sciences, Heidi B Kaplan, Elizabeth A Shank, Gisela Storz Aug 2025

An Exciting Future For Microbial Molecular Biology And Physiology, Andrew A Bridges, Leah Guthrie, Mckenzie Lehman, Elizabeth H Kellogg, Samantha Wellington Miranda, Andrew Pountain, Anthony L Shiver, Andrew Varble, Molecular Biology And Physiology Community Of The Council On Microbial Sciences, Heidi B Kaplan, Elizabeth A Shank, Gisela Storz

Faculty, Staff and Student Publications

Continuous advances in technologies ranging from deep sequencing and genetic manipulation to mass spectrometry, single cell imaging, and structural biology have led to previously unimaginable advances in our understanding of microbial physiology and the molecular mechanisms underlying microbial responses in a multitude of environments. Simultaneously, these advances are revealing how much more there is to learn. At the 2024 virtual retreat of the Molecular Biology and Physiology (MBP) Community of the Council on Microbial Sciences (COMS) of the American Society for Microbiology (ASM), eight early-career investigators, along with retreat attendees, discussed some of these astounding advances, as well as the …