Digital Commons Network^™

Molecular Characterization Of Humic And Fulvic Acids On Watterlogged And Well-Drained Amazonian Podzols, Amanda M. Tadini, Aleksandar I. Goranov, Stéphane Mounier, Débora M.B.P. Milori, Célia R. Montes, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

The Amazon rainforest is the largest tropical rainforest in the world. Amazonian Podzol soils, characteristic of this region, are known to store substantial amounts of organic carbon in both their surface and deep horizons. Despite decades of research, the molecular-level composition of these soils remains uncharacterized. This study addresses this knowledge gap by employing ultrahigh resolution mass spectrometry, namely, Fourier transform–ion cyclotron resonance–mass spectrometry (FT-ICR-MS), to determine the molecular composition of humic acid (HA) and fulvic acid (FA) fractions from two Amazonian Podzol profiles of varying levels of groundwater exposure (waterlogged vs well-drained). In the waterlogged soil compounds containing nitrogen, …

Deciphering The Chemistry Of Condensed Aromatic "Black" Carbon And Nitrogen In Amazonian Anthrosols, João Vitor Dos Santos, Aleksandar I. Goranov, Laís G. Fregolente, Marcia C. Bisinoti, Zhenhuan Sun, Klaus Schmidt-Rohr, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

Amazonian anthrosols are renowned for their high fertility and dark color, properties primarily attributed to the abundance of condensed aromatic carbon (ConAC) in the soil organic matter. ConAC, commonly referred to as black carbon, play a key role in the stability and nutrient retention of these soils. However, the processes governing the formation of ConAC and its transformation into oxygenated derivatives remain poorly understood. In this study, we used multiple analytical platforms to investigate the chemistry of ConAC-rich humic acids (HA) extracted from Terra Mulata de Indio, a type of Amazonian anthrosol. The results reveal that ConAC are predominantly …

Experimental Demonstration And Study Of Transmembrane-Electrostatically Localized Protons Prevail, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

In this review in relation to some interesting arguments from Silverstein, it is now reaffirmed that the experimental demonstration of transmembrane-electrostatically localized protons (TELPs) was successfully accomplished through 1) using an aluminum film as a TELPs sensor in a biomimetic anode water-membrane-water cathode system, 2) analyzing the water-electrolysis current curve, and 3) measuring bulk liquid phase pH. Formation of induced TELPs was subsequently discovered in an anode water-membrane-water-membrane-water cathode system. The discovery of induced TELPs enabled clean experimental tests on the cation-TELPs exchange process by using sodium bicarbonate or potassium bicarbonate solution in the central liquid chamber. The sodium/TELPs exchange …

Decoding Tattoo And Permanent Makeup Pigments: Linking Physicochemical Properties To Absorption, Distribution, Metabolism, And Elimination Profiles Using Quantitative Structure-Activity Relationship (Qsar)-Based New Approach Methodologies (Nams), Girija Bansod, Ajay Vikram Singh, Preeti Bhardwaj, Tulika Rai, Sweta Vijay Nakhale, Amruta Shelar, Rajendra Patil, Peter Laux, Andreas Luch, Christopher J. Osgood, Michael W. Stacey

Biological Sciences Faculty Publications

The safety and quality of tattoo and permanent makeup (PMU) pigments are subject to increased scrutiny due to their potential to cause adverse effects like anaphylaxis, photoallergic responses, and long-term toxicity. These undesirable reactions governed by their chemical structure possess varied physicochemical properties and absorption, distribution, metabolism, and elimination (ADME) characteristics. These properties control the pigment behavior during application, stability, and interaction with human tissue. The correlation between these physicochemical characteristics and ADME parameters of tattoo/PMU pigments remain under-explored despite the current advances in toxicology. Our study aims to address and bridge the gap by leveraging open-access QSAR computational toxicology …

Frozen Green Leaves As Potential Nutrient Subsidies In North American Mangrove Ecosystems, Katelyn Overstreet, Emily M. Dangremond, Ilka C. Feller

Biological Sciences Faculty Publications

Avicennia germinans, the black mangrove, has shifted its range limit northward, and increased in abundance in the Southeastern United States. In January 2018, a 3-day freeze event caused substantial defoliation of green leaves in A. germinans near its northernmost range limit in St. Augustine, FL. During their recovery, plants that lost their leaves grew similarly to plants fertilized with nitrogen, leading to the hypothesis that freeze-killed green leaves (frozen green) may have acted as a fertilizer. To assess the value of frozen green litter as a nutrient subsidy, we performed an experiment in which A. germinans seedlings were grown …

Edge Effects Impact Blue Carbon Dynamics Across Coastal Ecotones In A Tropical Seascape, Erik S. Yando, Jahson B. Alemu I, Kiah Eng Lim, Taylor M. Sloey, Michiel Van Breugel, Natasha Bhatia, Daniel A. Friess

Biological Sciences Faculty Publications

Coastal wetlands are important for their ability to regulate global climate through the sequestration and long-term storage of carbon. Accurate quantification of ecosystem-specific carbon dynamics (including sequestration, storage, and fluxes) is needed to develop accurate carbon budgets that inform climate change mitigation. Most work to quantify carbon dynamics either use subsampling in core habitats or benefit transfers to upscale values. While these approaches are valuable, our understanding of carbon dynamics across ecosystem transitions and overall heterogeneity remains a critical gap in coastal ecosystems as boundaries are not always clear. In this study, we established transects across both mangrove and seagrass …

Assemblage Structure Of Large Carnivorous Coral Reef Fishes At Shallow And Mesophotic Depths In Two Of The Largest And Most Isolated Marine Protected Areas In The Philippines, Rene A. Abesamis, Mikaela L. Salvador, Ryan Murray, Kymry Delijero, Segundo F. Conales, Jean T. Utzurrum, Christopher E. Bird, David T. Gauthier

Biological Sciences Faculty Publications

Overfishing remains a threat to coral reef fishes globally, with large carnivores often the most vulnerable. Marine protected areas (MPAs) can restore fish populations and biodiversity, but their impact has been understudied in mesophotic coral ecosystems (MCEs – extensions of shallow reefs from 30 to 150 m). We analyzed baited remote underwater videos recorded in 2016 to investigate the assemblage structure of large carnivorous fishes at shallow (4–12 m) and mesophotic (45–96 m) depths in two of the largest and most isolated MPAs in the Philippines: Tubbataha Reefs Natural Park (TRNP), a fully no-take MPA enacted in 1988; and Cagayancillo, …

Building Heat-Resilient Caribbean Reefs: Integrating Thermal Thresholds And Coral Colonies Selection In Restoration, Macarena Blanco Pimentel, Johanna Calle-Triviño, Daniel J. Barshis, Sancia E. T. Van Der Meij, Megan K. Morikawa

Biological Sciences Faculty Publications

Caribbean reefs face increasingly frequent and intense bleaching events, adding to the numerous other threats impacting these ecosystems. Addressing these challenges requires global action to reduce climate drivers, along with local efforts like reef restoration. Active restoration using thermotolerant coral colonies offers a potential strategy to alleviate these impacts; however, gaps remain in identifying context-specific temperature thresholds to guide colony selection and standardize thermotolerance assessment methods. This study addressed these gaps in two phases. First, by determining practical thresholds to differentiate species responses to heat stress; and second, by developing a framework to identify and prioritize resilient colonies for restoration. …

First Reference Genomes For Two Mesophotic Reef Building Coral Species: Leptoseris Cf. Scabra And Montipora Cf. Grisea, Veronica Z. Radice, Johanna C. Gijsbers, Silvia Vimercati, Daniel J. Barshis

Biological Sciences Faculty Publications

Coral mortality is occurring worldwide at an alarming rate. Despite the immense and underestimated biodiversity of reef-building corals, very few genomes are available. Further, almost all genomic resources originate from shallow water corals even though photosynthetic, symbiotic corals occur at mesophotic depths deeper than 30 m and even >100 m. We present annotated, de novo genomes for two mesophotic, scleractinian (reef-building) corals Montipora cf. grisea and Leptoseris cf. scabra from American Sāmoa, the latter being the first genome for the widespread genus Leptoseris. We used PacBio continuous long reads and Omni-C data to assemble chromosome-level reference genomes. For Montipora …

Validating A Novel Capability Of Assessing Pathways Of Animal Water Gain And Loss, Zachary T. Steele, Karen Caceres, Zachary A. David, Lisa M. Shollenberger, Alexander R. Gerson, Seth D. Newsome, John P. Whiteman

Biological Sciences Faculty Publications

Understanding variations in the routes by which wild animals gain and lose water is challenging, and common methods require longitudinal sampling, which can be prohibitive. However, a new approach uses Δ′¹⁷O_BW (Δ′¹⁷O of animal body water), calculated from measurements of δ′¹⁷O and δ′¹⁸O in a single sample, as a natural tracer of water flux. Δ′¹⁷O_BW is promising, but its relationship to organismal variables such as metabolic rate and water intake have not been validated. Here, we continuously measured oxygen influxes and effluxes of captive deer mice (Peromyscus maniculatus), and manipulated their water intake and metabolic rate. We used …

Hair Growth Rate Estimation In North American Ursids, Jennifer H. Stern, Karyn D. Rode, Craig A. Stricker, Sheriden Ploof, Cindy L. J. Roberts, Celess Edinger, Mary Humbyrd, Nate Wagner, Megan A. Owen, John P. Whiteman, Thea Bechshoft, Brent White, Kristin L. Laidre

Biological Sciences Faculty Publications

The feeding ecology of wildlife populations has important implications for individual health, population productivity and distribution patterns. For ursids (bears), food resources and feeding behaviour primarily affect population dynamics via effects on cub production and survival. Much of what is known about the feeding ecology of bears is based on analyses of tissues collected from capture-based research efforts, harvested animals or non-invasive approaches. However, inference about diet from hair has been limited by a lack of quantitative data on the timing of the moult and hair growth rates. We conducted a study to develop and test two methods of quantifying …

Future-Oriented Coastal Protection: The Utility Of Living Shorelines Under Changing Climatic Conditions, Gabriella R. Dipetto, Donna Marie Bilkovic, Taylor M. Sloey, Erik S. Yando, Erik L. Walters

Biological Sciences Faculty Publications

An accumulation of novel nature-based shoreline management techniques have developed over the past two decades out of the continued need to protect waterfront land combined with an awareness of the relationship between healthy coastal ecosystems and human societies. Shoreline protection strategies such as living shorelines incorporate these nature-based approaches to simultaneously enhance ecosystem functioning while providing shoreline stabilization benefits. The continued role of these approaches into the future, however, will be challenged by a rapidly changing coastal environment. While living shorelines have received global attention and integration into climate policy and governance frameworks, the ability of shoreline systems to withstand …

The Pluralistic Natural Capital Values Of A Tropical City, Adrienne Grêt-Regamey, Justine Saunders, Peter Edwards, Daniel Richards, Jahson I. Alemu, Natasha Bhatia, Roman Carrasco, Zuzana Drillet, Tze Kwan Fung, Yan Feng Leon Gaw, Wanggi Jaung, Andrea Law, Rachel Ai Ting Leong, Aikeen Youu Ming Lim, Mahyar Masoudi, Yudhishthra Nathan, Rachel Rui Ying Oh, Wen Ting Ooi, Fairul Edros Ahmad Shaikh Shaikh, Xiao Ping Song, Claudia L.Y. Tan, Puay Yok Tan, Sergio Wicki, Lynn-Wei Wong, Yanyun Yan, Erik Yando, Alex Thiam Koon Yee, Jingyuan Zhang, Daniel A. Friess

Biological Sciences Faculty Publications

Nature in cities is essential for human well-being. Quantifying and valuing the goods and services provided by nature to city dwellers is missing in tropical contexts. Yet, as cities worldwide face similar challenges, understanding the services provided by tropical urban ecosystems becomes imperative for effective management. Here, we present the first Natural Capital Assessment of a tropical city, unveiling three critical insights. Firstly, we demonstrate the vital reliance of a developed tropical city on nature, particularly for climate change mitigation through regulating services. Secondly, we identify intact natural areas as Singapore’s most valuable assets, stressing the significance of the quality …

Emerging Nanotechnology Approaches For Blood Disorders: A Comprehensive Review Of Nano-Hematinics And Plant-Based Nanomedicines, Hassan Abdulsalam Adewuyi, Khairat Shamsudeen, Fatima Mahmoud Muhammad, Adeola Kolawole Victor, Sakariyau Adio Waheed, Adepeju Matilda Adekoya, Kashim Ibrahim Muhammad, Blessing Temitayo Longe, Abdulgaffar Abdulquddus

Chemistry & Biochemistry Faculty Publications

Background: Disorders affecting blood components, such as anemia, coagulopathies, and hematologic malignancies, continue to pose significant global health burdens. Traditional therapies often fall short due to adverse effects, limited bioavailability, and insufficient targeting of disease sites.

Objective: This review synthesizes current advances in nano-hematinics and plant-based nanomedicines (phyto-nanomedicines) as innovative strategies for managing blood-related conditions. These nanoscale interventions are designed to enhance therapeutic precision, bioactivity, and safety.

Methods: A systematic review was conducted using major scientific databases including PubMed, Scopus, Web of Science, and ScienceDirect. Search terms were carefully selected to retrieve literature published between 2015 and 2024 that discussed …

Oxidation Camouflages Terrestrial Organic Matter To Appear Marine-Like, Aleksandar I. Goranov, Susan J. Carter, Ann Pearson, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

The export of terrestrial organic matter (TOM) to the ocean has been traditionally viewed to be minimal or only of significance in near-coastal continental margins. The broadly accepted explanation for the widespread loss of terrestrial lignin biomarkers and apparent disappearance of the <-25‰ stable carbon isotopic signature (δ¹³C) of TOM is that TOM is almost fully degraded before reaching the open ocean. Oceanic carbon (δ¹³C value ≥ -22‰) is therefore thought to be derived primarily from algae. However, an alternative explanation for the different molecular and δ¹³C signatures in terrestrial and marine environments may be that oxidative processing transforms TOM to appear marine-like. To test this hypothesis, we subjected eight TOM samples to a strong oxidation gradient. At carbon losses typical of TOM degradation during transport and sedimentation (above 60%), the differentiators of provenance disappeared, leaving a residue that was marine-like both chemically (aliphatic- and nitrogen-rich) and isotopically (δ¹³C enrichment of 4 to 9‰). This challenges the validity of conventional two-endmember mixing models, suggesting that a much larger fraction than previously estimated of the organic matter found in the ocean may originate from terrestrial sources, impacting global models of carbon cycling and sequestration.

The Effect Of Need For Closure, Gender Essentialism, And Contact On Transphobia, Jacob E. Vargas

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Using a motivated social cognition framework, this study explores whether there is an indirect effect of need for closure on transphobia via gender essentialism and whether this relationship is moderated by transgender contact. Data were collected from an undergraduate sample of 405 cisgender participants (n_men = 159, n_women = 246). A simple mediation and a moderated mediation were used to examine the hypothesized relationships, controlling for participant gender. Results reinforced the positive correlation between gender essentialism and transphobia and the negative correlation between transgender contact and transphobia. However, the mediation and moderation hypotheses were not supported. Need …

Examining Trauma And Substance Use Co-Morbidity In African Americans, Breanne Maray Sande Russell

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Childhood maltreatment (CM) is a profound public health crisis that significantly elevates the risk for substance use disorders (SUDs), including alcohol and opioid misuse. Adolescents exposed to CM are especially vulnerable, often turning to substances as maladaptive coping mechanisms during critical developmental periods. African American youth bear this disproportionate burden, yet research addressing their unique experiences remains strikingly limited. Furthermore, while the co-occurrence of trauma-related disorders and substance use is well documented, racial and ethnic minority groups continue to be underrepresented in this literature. Emotion regulation difficulties are increasingly recognized as a mechanism linking PTSD symptoms with substance use, yet …

Robust Quantification Of Cortical Hemodynamic Response To Tactile Stimulation: A Comprehensive Fnirs Methodology To Mitigate Physiological Confounds, Mohsen Hozan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation establishes a quantitative framework for studying somatosensory processing using functional Near-Infrared Spectroscopy (fNIRS), with a focus on applications in neurorehabilitation. The central aim is to investigate how the brain's hemodynamic response is modulated by the velocity of patterned tactile stimulation. While fNIRS is a promising neuroimaging tool, its data quality is often compromised by physiological noise and motion artifacts, challenging the reliability of its findings and hindering the development of effective, quantifiable neurotherapeutics.

Two preliminary investigations informed the final experimental design. The first pilot study confirmed that pneumotactile stimulation of the hand, during both passive (somatosensory) and active …

Fire History, Ecological History, And Historical Human-Environment Interactions In The Northern Great Plains, James Victor Benes

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation explores the spatiotemporal signatures of fire in North American grassland ecosystems. In three chapters, I explore different aspects of fire history in the northern Great Plains. Chapter 1 is an introduction. Chapter 2 focuses on the Euro-American Settlement Period (ca. 1850–1950 C.E.). Fire histories from six different lakes in the Nebraska Sandhills are compared with two periods of historic drought and the establishment of United States Post Offices. Five of the six records show increased levels of biomass burning during the initial phases of Euro-American Settlement. Chapter 3 is a long-term (ca. 15,000 years), high-resolution fire history and …

Scientific Writing: A Guide From Data To Draft, Kristin M. Klucevsek

Open Education Materials

Textbook for ENGL302W (Scientific Writing)

CC BY-NC-SA (2025)

Paternal Involvement And Bonding In Neonatal Intensive Care Unit: Evaluating Daily Caregiving, Emotional Well-Being, And Father-Infant Connection, Ava G. Kinyon

Nursing | Student Research Posters

Background: Premature birth and serious neonatal conditions frequently require infants to obtain care in the NICU, producing psychological and emotional obstacles for families. Despite the fact that skin-to-skin contact has been extensively researched for its strengths in encouraging stability and bonding, there persists restricted importance and understanding on paternal participation in routine caregiving tasks such as feeding, diapering, temperature checks, and swaddling. Purpose: This study aims to evaluate paternal participation in daily NICU caregiving tasks to address gaps in comprehending how these experiences impact bonding and emotional well-being among fathers of hospitalized infants. Methods: This study will …

Knee Health Is A Major Determinant Of Mobility Across The Healthspan, Brandon M. Peoples, Kenneth Harrison, Michael A. Samaan, Christopher B. Mobley, David T. Redden, Jaimie A. Roper

Kinesiology and Health Promotion Faculty Publications

Knee health constitutes a pivotal determinant of locomotor function and overall mobility throughout the healthspan. Impairments in knee function lead to a series of biomechanical and physiological alterations that pose substantial public health challenges and economic burdens worldwide. This review synthesizes evidence that delineates the complex and multifaceted relationship between knee health and mobility, emphasizing the interplay across various domains in adult populations. Knee health is conceptualized as a dynamic, interconnected system whereby structural integrity, neuromuscular mechanisms, biomechanical adaptations, and functional mobility are intricately interrelated through cascades of mechanistic processes operating across different temporal stages. A comprehensive understanding of these …

This Is How You Can Be A Cool Adult, Angelica Angelica

ENGS 15.11: Design and Education

“This Is How You Can Be a COOL Adult” is an interactive and self-reflective lesson designed to help high school juniors through college seniors practice being more compassionate, open-minded, optimistic, and laughing-out-loud (humorous) in their everyday lives. By the end of this lesson, students will be able to describe and explore research-based strategies that foster compassion, open-mindedness, optimism, and humor, and create a personalized “COOL Action Plan” with self-reflection logs to track progress. The lesson takes place in an unconventional classroom setting (outdoors) and includes mini-lectures, meditation, collaborative games, creative role-play, personal reflection, and practical methods for applying these qualities …

Averting "Death By Powerpoint", Nora Xinyi Cai

ENGS 15.11: Design and Education

This lesson empowers students to design presentation slides that effectively communicate their ideas to unfamiliar audiences. The lesson emphasizes the key guiding principle for slide design, one message per slide, along with four steps to implement it. Students will develop the vocabulary to assess the quality of others' presentation slides and apply these four steps in creating their own. The lesson seeks to cultivate a dynamic, collaborative, and creative learning environment by design.

Refundable Tax Credit Design: Cohabitating Parents And Gender Presumptions, Michelle Lyon Drumbl

Scholarly Articles

This Essay is meant to encourage further thinking to improve upon design options for unmarried cohabitating parents. Are there better ways to recognize the needs of unmarried co-parents (whether living together or not) and to provide support for shared parenting? The policy considerations for providing support to unmarried cohabitating parents are different than those for supporting married couples, given that these individuals lack the financial protections of a formal partnership.

One solution is to have a default rule whereby the CTC is awarded to the primary caregiver. Canada’s gender presumption provides a degree of administrative simplicity, but the IRS could …

Systematic Review Of Immunohistochemistry Protocols, Arpitha Parthasarathy

Pitzer Senior Theses

Immunohistochemistry (IHC) is an essential technique in biological research that allows for the visualization and localization of proteins within tissue samples. This systematic review analyzes the most effective, efficient and reproducible protocols for IHC. It focuses on three critical steps: freezing, cryosectioning, and staining of skeletal muscle tissues in mice models. A total of 41, 44, and 48 articles on freezing, cryosectioning, and staining techniques, respectively, were analyzed, from reputable sources in biology like PubMed and Web of Science. Results showed the following. For freezing, the use of isopentane combined with liquid nitrogen appeared to be the best method, minimizing …

Crisis In The Current: A Proposed Study On The Impact Of Columbia River Basin Water Quality On Juvenile Chinook Salmon Survival, Olivia Buist-Thuillier

Pitzer Senior Theses

Salmon occupy a vital role in the ecosystem, economy, and indigenous culture of the Pacific Northwest of the United States, specifically within the Columbia River Basin. Of the five species of Pacific Salmon that live in the basin, Chinook salmon (Oncorhynchus tshawytscha) are the most widely commercially fished and are the largest. In recent years, Chinook Salmon populations have heavily declined from human influence. Rising temperatures from anthropogenic climate change, habitat destruction (dams, human presence), runoff from agricultural practices, and overfishing have all contributed to this decline. Because of their role as a keystone species and their cultural significance, there …

Valuating The Impact Of Unexploded Ordnance (Uxo) In Savannakhet, Laos Through Ecosystem Services Assessments, Nathan Lu

Pitzer Senior Theses

Unexploded ordnance (UXO) impact on countries is not well-documented, since post-war surveys or data collection are often hindered by a lack of appropriate funding and reparation. In Laos, UXO has hardly been cleared since 1964 and thus leaves room for spatially and temporally analyzing the severity of environmental and economic degradation in the area. This study uses the framework of ecosystem services, particularly carbon stock, as a way to provide quantitative metrics of impact to local governments and stakeholders. Through remote sensing data in Savannakhet Province, evidence-based land-use change data was spatially analyzed with geocoded UXO bomb mission locations and …

The Cognition Of Emotion: How Cognitive Control And Working Memory Underly Emotion Regulation Strategy Differences, Nathan Chabin

Pitzer Senior Theses

Working memory is crucial for comprehensively grasping the dynamics of emotion regulation (ER) and cognitive control, as it serves as a foundational cognitive resource that influences how individuals manage their thoughts, emotions, and behaviors. This research aimed to examine the role working memory capacity (WMC) has on Frontal Midline Theta (FMT), an EEG marker for cognitive control, during two common ER strategies: emotional suppression and cognitive reappraisal. Participants performed both an Operation Span working memory task and an ER task, where they viewed a series of negative and neutral images across three conditions. First, participants were instructed to passively view, …

To Seek And Sustain: A Theoretical Exploration And Choreographic Analysis Of Embodied Safety, Holand B. Dennis

Pitzer Senior Theses

The following project is a choreographic exploration framed by a literature review of the polyvagal theory, the BrainDance, and processes of regulation and co-regulation. Combined and interwoven, they demonstrate the potentiality of embodied safety. The performative work, “To Seek and Sustain”, celebrates a collective authorship of movement, emphasizes process over product, and centers dancers' subjective experiences. The polyvagal theory and the BrainDance were the catalyst for this piece; their descriptions drove the choreographic intentions and the dance’s expressive nature. “To Seek and Sustain” demonstrates how to identify and embody physiological safety through choreographed movement.