Digital Commons Network^™

Bs Wildlife, Fisheries, And Conservation, Fisheries 2025-26, Arkansas State University - Jonesboro

Bachelor of Science and Education

No abstract provided.

Bs Engineering Technology, Technical 2025-26, Arkansas State University - Jonesboro

Bachelor of Science and Education

Technical Studies Emphasis

Bs Environmental Science, 2025-26, Arkansas State University - Jonesboro

Bachelor of Science and Education

No abstract provided.

Bs Biotechnology, 2025-26, Arkansas State University - Jonesboro

Bachelor of Science and Education

No abstract provided.

Bs Biological Science, Pre-Professional 2025-26, Arkansas State University - Jonesboro

Bachelor of Science and Education

No abstract provided.

Development Of Self-Healing Polymer From Bio-Based Benzoxazine Blend With Poly(Ethylene Glycol) For Anti-Biofouling Applications, Pharaporn Yodkum

Chulalongkorn University Theses and Dissertations (Chula ETD)

Biofouling remains a significant challenge in marine industries, as conventional coatings often lose their antifouling efficacy once they become damaged. Incorporating self-healing capabilities into antifouling coatings has therefore emerged as a promising strategy to extend service life and maintain long-term performance. In this study, self-healing polymers were developed based on a bio-derived benzoxazine resin (E-fa), synthesized from renewable precursors including eugenol (a phenol source), furfurylamine (an amine source), and paraformaldehyde, and subsequently blended with poly(ethylene glycol) (PEG). The blends were formulated with varying weight ratios (95/5, 90/10, 85/15, and 80/20 wt/wt%) and PEG molecular weights (PEG-600, PEG-1500, and PEG-4000). This …

Comparative Analysis Of Gut Microbiota In Post-Mortem Chronic Alcoholics Versus Minimal To No Alcohol Users In The Thai Population, Laiba Pervez

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chronic alcohol consumption induces long-term disruption of the gut microbiota, contributing to inflammation, metabolic imbalance, and impaired intestinal integrity. This study investigated 24 postmortem gut microbiota samples from the Thai population, including 12 chronic alcoholic and 12 non-alcoholic cadavers, focusing on cecal and fecal samples to identify site-specific microbial signatures of alcohol-associated dysbiosis. Following DNA extraction, the V3–V4 regions of the 16S rRNA gene were sequenced on the Illumina MiSeq platform. Data were processed in QIIME for OTU clustering and taxonomic profiling. Alpha (Shannon, Chao1, ACE) and beta (Bray–Curtis) diversity were analyzed with PERMANOVA (p< 0.05) while LEfSe, cladogram, and STAMP analyses identified differentially abundant taxa.. At the phylum level, alcoholic cecal samples (AC) showed a decrease in Firmicutes (59.07%) and increases in Proteobacteria (5.54%), Fusobacteria (8.48%), and Actinobacteria (2.78%) compared with controls (NAC). The rise in Actinobacteria was statistically significant. The class Bacilli was also elevated, reflecting a more oxygenated and inflammatory intestinal environment. At the genus level, Paeniclostridium was enriched in alcoholics, while the butyrate-producing Coprococcus was reduced, suggesting impaired SCFA metabolism and gut barrier function. In fecal samples (AF vs NAF), phylum-level differences were minimal, although Clostridium sensu stricto 1 was notably higher in alcoholics, consistent with inflammation-associated dysbiosis. When comparing gut regions, distinct spatial heterogeneity was observed. Within the alcoholic group, Firmicutes were more abundant in cecum, while Actinobacteria were lower compared to feces. Among controls, Actinobacteria and Bacteroidetes were enriched in feces, whereas Firmicutes predominated in the cecum. Alpha diversity analysis using ACE and Chao1 indices showed significant differences between cecal and fecal samples of the control group. Overall, postmortem gut microbiota retained distinct alcoholassociated signatures, with the cecum preserving clearer microbial patterns than feces. These findings highlight the cecum’s potential as a reliable site for detecting alcohol-related dysbiosis in forensics.

ผลของโปรแกรมเสริมสร้างความรอบรู้ด้านสุขภาพผ่านไลน์แอปพลิเคชันต่อพฤติกรรมการจัดการตนเองด้านการบริโภคอาหารในผู้ป่วยภาวะกล้ามเนื้อหัวใจขาดเลือดเฉียบพลันที่มีโรคร่วม, สุนิษา ละดา

Chulalongkorn University Theses and Dissertations (Chula ETD)

การวิจัยแบบกึ่งทดลองนี้มีวัตถุประสงค์ เพื่อศึกษาผลของโปรแกรมเสริมสร้างความรอบรู้ด้านสุขภาพผ่านไลน์แอปพลิเคชันต่อพฤติกรรมการจัดการตนเองด้านการบริโภคอาหารในผู้ป่วยภาวะกล้ามเนื้อหัวใจขาดเลือดเฉียบพลันที่มีโรคร่วม เบาหวาน ความดันโลหิตสูง ไขมันในเลือด 1 โรคขึ้นไป ที่มีอายุตั้งแต่ 35 – 80 ปี คัดเลือกกลุ่มตัวอย่างแบบเฉพาะเจาะจง แบ่งเป็นกลุ่มควบคุมและกลุ่มทดลอง กลุ่มละ 23 คน จับคู่ทั้งสองกลุ่มให้มีลักษณะคล้ายคลึงกันในเรื่องอายุ จำนวนโรคร่วม จำนวนกลุ่มยาที่ใช้รักษาโรค เปรียบเทียบข้อมูลพื้นฐานด้วยสถิติ Chi-square test และ Fisher’s Exact Test กลุ่มตัวอย่างทั้งสองกลุ่มมีคุณลักษณะพื้นฐานที่ไม่แตกต่างกันอย่างมีนัยสำคัญทางสถิติในทุกตัวแปร (p > .05) กลุ่มควบคุมจะได้รับการพยาบาลปกติ ในขณะที่กลุ่มทดลองได้รับโปรแกรมเสริมสร้างความรอบรู้ด้านสุขภาพผ่านไลน์แอปพลิเคชัน เครื่องมือที่ใช้ในการวิจัย ได้แก่ แบบสอบถามพฤติกรรมการจัดการตนเองด้านการบริโภคอาหารของผู้ป่วยที่มีภาวะกล้ามเนื้อหัวใจขาดเลือดเฉียบพลันที่มีโรคร่วม และแบบสอบถามประเมินความรอบรู้ด้านสุขภาพเกี่ยวกับพฤติกรรมการจัดการตนเองด้านการบริโภคอาหาร ทดสอบความตรงเชิงเนื้อหาโดยผู้ทรงคุณวุฒิ 5 คน ได้ค่าดัชนีความตรงตามเนื้อหาเท่ากับ 0.92 และ 1.00 และค่าความเที่ยงเท่ากับ 0.91 และ 0.73 วิเคราะห์ข้อมูลด้วยสถิติเชิงพรรณนา และสถิตทดสอบค่าที ผลการวิจัยสรุปได้ดังนี้ 1. คะแนนเฉลี่ยพฤติกรรมการจัดการตนเองด้านการบริโภคอาหารในผู้ป่วยภาวะกล้ามเนื้อหัวใจขาดเลือดเฉียบพลันที่มีโรคร่วม กลุ่มทดลองหลังได้รับโปรแกรมเสริมสร้างความรอบรู้ด้านสุขภาพผ่านไลน์แอปพลิเคชันสูงกว่าก่อนได้รับโปรแกรม ฯ อย่างมีนัยสำคัญทางสถิติที่ระดับ .05 2. คะแนนเฉลี่ยพฤติกรรมการจัดการตนเองด้านการบริโภคอาหารในผู้ป่วยภาวะกล้ามเนื้อหัวใจขาดเลือดเฉียบพลันที่มีโรคร่วม ที่ได้รับโปรแกรมเสริมสร้างความรอบรู้ด้านสุขภาพผ่านไลน์แอปพลิเคชันสูงกว่ากลุ่มที่ได้รับการพยาบาลตามปกติอย่างมีนัยสำคัญทางสถิติที่ระดับ .05

Umn Morris Telephone Extensions 2025-2026, University Of Minnesota - Morris

UMN Morris Directories

No abstract provided.

Attention Deficit Hyperactivity Disorder And Metacognitive Awareness Of Learning, Kara R. Muscha

Theses

The present study examined knowledge updating abilities in individuals with and without ADHD when forced to metacognitively evaluate the effectiveness of different study strategies. Participants with and without ADHD were randomly assigned to either a spontaneous or a forced knowledge updating condition, with those in the latter condition being asked to keep track of encoding strategy-recall outcome results, but no additional instructions for those in the spontaneous condition. I expected that healthy control participants would show greater knowledge updating abilities than those with ADHD, the participants in the forced knowledge updating condition would show greater knowledge updating abilities than participants …

Modifying The L-Tryptophan Affinity Of The Ribosomal Arrest Peptide Tnac, Alexis Orlando Delgado Castillo

Theses

Since creating biosensors from zero has proven a more challenging enterprise than expected, resorting into naturally occurring regulatory systems already characterized represents a more accurate, rapid, and attractive option. Various previous strategies have sought to exploit and modify regulatory elements that sense for different molecules, including monosaccharides (Tang et al., 2008), secondary metabolites (Flachbart et al., 2021), and other amino acids (Della Corte et al., 2020). This study aims to leverage current scientific knowledge and advances concerning the structure and function of the ribosomal arrest peptide TnaC. With this research I integrated the TnaC ribosome-arresting peptide into a new biosensor …

Aviation Weather Forecasting Utilizing An Artificial Neural Network, Joshua Mote

Theses

Weather forecasting is critical to minimize risk and maximize efficiency for flying operations and is challenging due to the uncertainty involved in atmospheric changes. Operational area weather is becoming more critical with the growing reliance on air domain for transportation. This study investigates whether the forecasting accuracy using a Hybrid Long-Short Term Memory (LSTM) Artificial Neural Network (ANN) outperforms the San Antonio International Airport (KSAT) Terminal Aerodrome Forecast (TAF) by integrating diverse data sources – regional Meteorological Aerodrome Report (METAR), TAF, Avian Advisory System (AHAS) Data and geomagnetic activity K Index Data. When tested on one year of historical data, …

Surveying Subterranean Biodiversity Of The Tongass National Forest Using Traditional Survey Approaches And Environmental Dna (Edna) Metabarcoding, Jared P. Higgs

Theses

The Tongass National Forest (TNF) is a large temperate rainforest in southeastern Alaska, much of which is underlain by karst. Timber harvest, among other threats, can have detrimental effects on karst habitats; however, the subterranean fauna of this region is understudied and lacks a baseline for monitoring. I spent three field seasons conducting biological assessments of caves in the TNF using two approaches: traditional specimen-based sampling and eDNA metabarcoding. To improve the reference sequence database, I generated 213 novel sequences from invertebrate specimens collected from 35 caves. I also collected 125 water samples from 39 sites for environmental DNA (eDNA) …

The Need To Explore The Potential Of Marine Cdr With A One-Earth Strategy: A Guide For Policy-Makers, Philip W. Boyd, Jean-Pierre Gattuso, Minhan Dai, Louis Legendre, Terre Satterfield, Romany M. Webb

Sabin Center for Climate Change Law

Rapid, deep and sustained reductions in carbon dioxide (CO₂) emissions are essential to achieve the goals of the Paris Climate Agreement of keeping the long-term global average surface temperature increase well below 2°C above pre-industrial levels and pursue efforts to limit it to 1.5°C . In addition, the 2021 IPCC Report explains that carbon dioxide removal (CDR) will be needed to offset residual CO₂ emissions from activities and sectors that are difficult to decarbonize by 2050. The objective of CDR is removal of atmospheric CO2 from residual emissions and its durable storage in reservoirs, which is an additional critical element …

Investigating The Plasticity And Role Of Peripherally Derived Cd4 T Cells In The Brain At Homeostasis, Tomomi Marie Yoshida

Yale Graduate School of Arts and Sciences Dissertations

Specialized immune cells residing in tissues orchestrate diverse biological functions through communication with parenchymal cells. The contribution of the innate immune compartment in the meninges and central nervous system (CNS) is well-characterized, however, whether T cells as part of the adaptive immune system reside in the brain and are involved in homeostasis is poorly understood. Here, we identify that the subfornical organ (SFO) region of the brain is a nucleus for parenchymal T cells in the steady-state brain in both mice and humans. Using unbiased transcriptomics, we found that these extravascular T cells in the mouse brain are distinct from …

Host Defense Inside & Outside Of The Immune System: Modeling Antimicrobial Immunity By Gbps, Zhongyu Yuan

Yale Graduate School of Arts and Sciences Dissertations

Traditional views of host defense often focus on antibody-mediated neutralization, cytotoxic T-cell plus NK-cell-mediated killing, and phagocytic activities by cell populations originating in the bone marrow as part of the classical immune system. Additional effector activities, however, also operate in non-immune cells which compose the stroma or parenchyma of host tissues. These latter cell populations often mobilize antimicrobial activities in response to cytokines such as type II IFN (IFN-gamma; IFN-) against a wide variety of intracellular pathogens.

Among the most prominent IFN--stimulated genes are a family of immune dynamin-like GTPases termed the Guanylate Binding Proteins (GBPs). GBPs are widely expressed …

The University Of Texas Permian Basin 2025-2026 Undergraduate Catalog 2025-2026, The University Of Texas Permian Basin

Course Catalogs

No abstract provided.

The University Of Texas Permian Basin 2025-2026 Graduate Studies Catalog, The University Of Texas Permian Basin

Course Catalogs

No abstract provided.

Baseline Disease Characteristics By Surgery History Among Patients With Chronic Rhinosinusitis With Nasal Polyps (Crswnp) In The Global Aroma Registry, Shigeharu Fujieda, Jayant M. Pinto, David W. Jang, Joseph K. Han, James Mims, Eugenio De Corso, Martin Wagenmann, Changming Xia, Thomas Plucinak, Mark Corbett, Scott Nash, Amr Radwan

Department of Otolaryngology (ENT) Faculty Publications

Purpose

The global Assessing long-teRm Outcomes in dupiluMAb (AROMA) registry study aims to characterize the long-term, real-world use of dupilumab in patients with chronic rhinosinusitis with nasal polyps (CRSwNP). This paper reports interim analysis of the baseline characteristics for the first 303 patients enrolled in AROMA.

Methods and materials

AROMA is currently ongoing in adult patients with CRSwNP who initiated dupilumab for up to 36 months. This interim analysis describes baseline demographics; systemic/oral corticosteroid (SCS/OCS) and antibiotic use; disease burden; healthcare resource utilization; and medical history. All outcomes are summarized descriptively.

Results

As of February 2023, AROMA had enrolled 303 …

Size Of Pediatric Tracheostomy Tube And Predictors Of Postoperative Complications, Soukaina Eljamri, Jordyn Lucas, Amber Shaffer, Basil Hashimi, Marina Rushchak, Reema Padia

Department of Otolaryngology (ENT) Faculty Publications

Objective

(1) To investigate weight as an alternative guide for trach tube choice. (2) Evaluate complications 3-month posttracheostomy and associations with patient and trach tube factors.

Study design

Retrospective cohort study.

Setting

Single academic medical center.

Methods

Patients < 2 years old who underwent tracheostomy placement from 2017 to 2022 were identified. Associations between trach characteristics, trach size/length, chest x-ray (CXR) and intraoperative endoscopy measurements, and complications within 3 months posttracheostomy placement were evaluated.

Results

In total, 68 patients were included in the study, with a median age of 4 months (range: 1 day to 1.74 years) and weight of 4.1 kg (range: 1.8-11.6 kg) at the time of the procedure. The length of the trachea measured on CXR was more closely associated with weight (ρ = 0.403, P = .0007) than with age (ρ = …

A Comparison Of Allergen Sensitization Profiles In Patients With Chronic Rhinosinusitis With And Without Nasal Polyposis, Lauren Trzcinski, Suhas Bharadwaj, Randall A. Bloch, Joseph K. Han, Kent K. Lam

Department of Otolaryngology (ENT) Faculty Publications

Chronic rhinosinusitis (CRS) and allergic rhinitis (AR) are common comorbid sinonasal conditions. CRS is classically divided into two distinct phenotypes: CRS with nasal polyposis (CRSwNP) and CRS without nasal polyposis (CRSsNP). The purpose of this retrospective observational study is to determine whether aeroallergen sensitization profiles in patients with comorbid CRS and AR can distinguish between CRSwNP and CRSsNP. A total of 241 patients diagnosed with comorbid CRS and AR who underwent skin prick testing or in vitro allergy testing in a single tertiary rhinology practice were included for evaluation. The rates of allergen-specific sensitizations in CRSwNP patients were compared with …

Liposomes As A Platform For Cd40 Ligand Presentation For The Purpose Of Feeder-Free B Cell Activation, Sydney Bone

Theses

Improvement of in-vitro culture of immune cells is critical for expanding our understanding of the immune system and advancing the treatment of diseases, whether pathogenic, cancerous, or autoimmune, via cellular immunotherapies. This study aims to develop a feeder-free B cell culture system using biomaterials-based presentation of a critical signaling molecule, namely CD40 ligand (CD40L). Traditional B cell culture methods rely on allogenic feeder cells to present CD40L, but a culture of only autologous cells is preferable for cellular immunotherapies. Previous work of our lab established an effective feeder-free B cell culture system using magnetic microbeads as a synthetic platform for …

Factors Affecting Freshwater Fish Communities In Two North Alabama Counties, Joshua Fitts

Theses

This study examined how physical habitat characteristics influence freshwater fish diversity in streams of Madison and Limestone Counties, Alabama. Ten 150-meter stream reaches were sampled using backpack electrofishing and seining, with habitat variables measured at fixed transects. Principal component analysis identified four main habitat gradients representing flow and substrate, channel size, gravel dominance, and canopy–bedrock conditions. Although overall species richness and Shannon diversity were not significantly related to these gradients, species-specific correlations revealed clear ecological preferences. Regression models showed that root wads, herbaceous vegetation, and riprap modestly increased richness, emphasizing the role of structural diversity. These findings highlight the importance …

Geophysics, And Capturing Ethnographic Interviews Of A Tidal Plantation Burial Ground, Kristin M. Meeuwen (Dew)

College of Graduate Studies: Theses & Dissertations

Plantation development in Chatham County has a rich but incomplete history. Silk Hope, an Ogeechee District tidal plantation, is located seven miles from the city of Savannah on the Northern banks of the Little Ogeechee River. This project focuses on identifying the site boundaries of portions of Silk Hope Plantation and its historical background. Specifically, the project focused on delineating the boundaries of the associated cemetery and/or burial grounds, while estimating the number of individuals interred there using ground-penetrating radar (GPR). Finally, collaborative work with community members, including stakeholder and descendant interviews, will provide ethnographic insights into the site's history …

Serious Bacterial Infections And Hepatitis C Virus Among People Who Inject Drugs, Thomas J. Stopka, Robin M. Nance, L. Sarah Mixson, Hunter Spencer, Judith I. Tsui, Judith M. Leahy, Mai T. Pho, Jean Dejace, Judith Feinberg, April M. Young, Wei Teng Yang, Amelia Baltes, Eric Romo, Randall T. Brown, Kerry Nolte, William C. Miller, William A. Zule, Wiley D. Jenkins, Joseph A. Delaney, Peter D. Friedmann

Epidemiology and Environmental Health Faculty Publications

Limited research has examined the possible synergistic interrelationships between serious bacterial infections (SBIs) of the heart (i.e., endocarditis), bone, spine, brain, or joints (e.g., osteomylelitis) and hepatitis C virus (HCV) infections. We examined whether syndemic interactions existed between SBI, HCV, and substance-use-related factors in rural communities, hypothesizing that injection-mediated risks elevated the likelihood for both SBIs and HCV infections, which could be exacerbated by synergistic biological–biological or biological and social interactions. We calculated the prevalence ratios (PRs) of past-year SBI associated with each risk factor in separate models. Effect modification among significant risk factors was assessed using multiplicative interaction. Among …

Viral Transcript And Tumor Immune Microenvironment-Based Transcriptomic Profiling Of Hpv-Associated Head And Neck Squamous Cell Carcinoma Identifies Subtypes Associated With Prognosis, Anastasiia Nikitina, Daria Kiriy, Andrey Tyshevich, Dmitry Tychinin, Zoya Antysheva, Anastasya Sobol, Vladimir Kushnarev, Nara Shin, Jessica H. Brown, James Lewis, Krystle A. Lang Kuhs, Robert Ferris, Lori Wirth, Nikita Kotlov, Daniel L. Faden

Epidemiology and Environmental Health Faculty Publications

Human papillomavirus (HPV)-associated head and neck squamous cell carcinoma (HPV-positive HNSCC) has distinct biological characteristics from HPV-negative HNSCC. Using an AI-based analytical platform on meta cohorts, we profiled expression patterns of viral transcripts and HPV viral genome integration, and classified the tumor microenvironment (TME). Unsupervised clustering analysis revealed five distinct and novel TME subtypes across patients (immune-enriched, highly immune and B-cell enriched, fibrotic, immune-desert, and immune-enriched luminal). These TME subtypes were highly correlated with patient prognosis. In order to understand specific factors associated with prognosis, we used the unsupervised clustering of an HPV-positive HNSCC cohort from The Cancer Genome Atlas …

Fairehr, Karen S. Galea, Finlay Brooker, Shahzad Rashid, Michael Bader, Yu Ait Bamai, Jos Bessems, Embialle Mengistie Beyene, Alison Connolly, Carla Costa, Pravina Deligannu, Radu Corneliu Duca, Kaoutar Chbihi, Kasahun Eba, Manosij Ghosh, Melissa Gonzales, Stuart Harrad, Erin N. Haynes, Nancy B. Hopf, Po Chin Huang, Kate Jones, Konstantinos M. Kasiotis, Ming Kei Chung, Jihyon Kil, Holger Koch, Jung Hwan Kwon, Elizabeth Ziying Lin, Henriqueta Louro, Kyriaki Machera

Epidemiology and Environmental Health Faculty Publications

The FAIREHR (Findable, Accessible, Interoperable, Reusable Environmental and Health Registry) platform is a state-of-the-art online registry for prospective harmonization of human biomonitoring (HBM). It was developed by the HBM working group of the Europe Regional Chapter of the International Society of Exposure Science (ISES Europe) and is supported by the HBM Global Network. FAIREHR is designed to harmonize HBM metadata and support the implementation of the FAIR (Findable, Accessible, Interoperable and Reusable) Guiding Principles throughout HBM studies or programs. The registry enables preregistration of HBM by capturing key metadata on study design, metadata management, and planned methods before participant recruitment. …

Monte Carlo Gradient Boosted Trees For Cancer Staging: A Machine Learning Approach, Audrey Eley, Thu Thu Hlaing, Daniel Breininger, Zarindokht Helforoush, Nezamoddin N. Kachouie

Mathematics and System Engineering Faculty Publications

Machine learning algorithms are commonly employed for classification and interpretation of high-dimensional data. The classification task is often broken down into two separate procedures, and different methods are applied to achieve accurate results and produce interpretable outcomes. First, an effective subset of high-dimensional features must be extracted and then the selected subset will be used to train a classifier. Gradient Boosted Trees (GBT) is an ensemble model and, particularly due to their robustness, ability to model complex nonlinear interactions, and feature interpretability, they are well suited for complex applications. XGBoost (eXtreme Gradient Boosting) is a high-performance implementation of GBT that …

Education About, In, And For Wetlands: Practices, Premises, Possibilities, And Challenges, Tom Marcinkowski

Mathematics and System Engineering Faculty Publications

The purpose of this paper is to review areas of educational theory, research, and practice relevant to wetlands education, notably education about, in, and for wetlands. Five key areas are reviewed: (a) from the Report of the Intergovernmental Conference on Environmental Education, goals and objectives for wetlands education; (b) from the literature, major curricular and instructional approaches in and elements of wetlands education; (c) from the literature, broad instructional strategies available to and commonly used in wetlands education; (d) from websites of federal agencies in the U.S., education, outreach, and training programs that focus on education about, in, and for …

Examining The Effects Of Songbird Nest Boxes And Habitat On Avian And Arthropod Communities In A Vineyard Ecosystem, Eleanor Macdonald

Cal Poly Humboldt theses and projects

Agricultural expansion threatens biodiversity, but integrating insectivorous birds into vineyard ecosystems may support both conservation and ecosystem services. We tested how nest boxes and habitat composition influence avian and arthropod communities in Napa Valley vineyards using a before-after, control-impact experiment across 20 sites. During the 2023-2024 breeding seasons we conducted avian point counts, sampled key pest and beneficial arthropods, and collected fecal samples from adult and nestling tree swallows (Tachycineta bicolor) and western bluebirds (Sialia mexicana) to detect vineyard pest DNA. Nest boxes significantly increased swallow and bluebird abundance within one year; swallow responses were strongest …