Digital Commons Network^™

Expression And Purification Of Prohibitin Proteins And Investigation Of Their Interaction With Mycobacterium Tuberculosis’S Virulence Factors, Zeina Rhaim

Open Access Theses & Dissertations

Prohibitin (PHB) is a highly conserved mitochondrial protein involved in cell cycle regulation, apoptosis, mitochondrial integrity, macrophage activation, cytokine production and many other critical cell functions. The prohibitin complex, formed by Prohibitin-1 (PHB1) and Prohibitin-2 (PHB2), functions as a mitochondrial membrane-associated receptor. Cancer, neurodegenerative disorders, and renal conditions were found to be linked to an irregular PHB expression. Emerging evidence has also highlighted a significant role for PHB in tuberculosis (TB), particularly through its interactions with Mycobacterium tuberculosis (Mtb) virulence determinants. The molecular mechanisms by which PHB influences TB pathogenesis remain poorly understood. Our preliminary studies showed that PHB1 was …

‘The Weight That We Carry’: Using Photovoice To Uncover Student Parents’ Experiences In Higher Education, Maria Fatima Rodriguez Velasquez

Open Access Theses & Dissertations

Caregiving students carry identities that have historically been marginalized within academia and continue to face limited institutional awareness and support (Lovell, 2020; McCourt, 2018; Yates, 2022). This study analyzes the experiences of student parents at the University of Texas at El Paso (UTEP) to understand how they balance their family and academic responsibilities, the challenges they face, and the communities of support they find within their university experience. Using Photovoice as a method, 22 student parents shared photographs and the narratives behind each image that represented their daily lives inside and outside the classroom. The data analysis, guided by grounded …

Relationships Between Social Determinants Of Health And Pain Outcome Changes After Exercise In Adults With Knee Osteoarthritis, Klarissa Siebert

Open Access Theses & Dissertations

Background: Knee osteoarthritis (KOA) is a common musculoskeletal disease with pain as the primary symptom. KOA pain is a major contributor to disability and reduced quality of life and has been associated with social determinants of health (SDOH) variables. As for treatments of KOA pain, exercise such as stationary cycling is a first-line and recommended treatment. However, limited research has examined how SDOH influence pain outcomes following such interventions. Objective: This study investigated whether income level, education level, employment status, acculturative stress, and perceived ethnic discrimination are associated with changes in pain following a standardized cycling ergometer exercise in adults …

What Limits Dryland Ecosystems? Patterns Of Soil Fertility And Resource Limitation In The Chihuahuan Desert, Dylan Stover

Open Access Theses & Dissertations

Human activities are substantially altering global resource cycles with widespread implications for biogeochemistry and ecosystem functioning globally. Drylands, regions where precipitation is outweighed by water losses, are especially sensitive to these large shifts in resource cycles due to their inherently low and variable resource availability. In these regions, resource availability and biological activity are often concentrated around plants – or fertile islands – and predominantly driven by pulses of water. However, our knowledge of the processes influencing biological productivity in drylands – patterns of soil fertility and resource limitation – remains lacking, and their unique biogeochemical and biological processes create …

Algebraic Approach To Data Processing: Techniques And Applications, Julio Urenda

Open Access Theses & Dissertations

In many areas of human knowledge, symmetries and invariances play an important role. In fundamental physics, starting with Relativity Theory, new physical theories have been formulated in terms of invariances and of the corresponding transformation groups – i.e., in terms what a mathematician would call an algebraic approach. In engineering, devices like wind tunnels, which are based on scale-invariance, enable us to test smaller-scale models of the actual designs. In biological sciences, symmetries and invariances are extremely important in analyzing the shape and functioning of living beings, from mammals to viruses. Invariance and symmetry – in the form of fairness …

Ode To The Infomercial, Edward Wade

Open Access Theses & Dissertations

'Ode to the Infomercial' is a poetry collection in five sections which explores themes of contemporary American life, parenthood, and the media landscape. While primarily written in a narrative voice, the poetic approach is as diverse as the content. Interwoven throughout the collection is religious imagery and political posturing accompanied by healthy doses of self-criticism and humor.

Associations Between School Experiences And Mental Health Outcomes By Gender Identity In Sexual And Gender Minority Youth In Texas And California, 2022 - 2024, Andrea Susana Yanez

Open Access Theses & Dissertations

Background: Depression and anxiety affect populations differently, with diagnoses disproportionately higher among sexual and gender minority groups, including LGBTQ+ youth. School experiences shape mental health; negative environments, bullying, and lack of support worsen outcomes, while affirming policies, peer support, and inclusive curricula are protective. Methods: This secondary analysis of the Family, Housing, and Me (FHAM) Project followed 83 LGBTQ+ youth from South Texas and California's Inland Empire (2022-2024). Data on sociodemographic characteristics, school experiences, and mental health outcomes were collected at baseline and at Waves I and II over 25 months. Analyses included descriptive statistics, monthly comparisons, and bivariate and …

Voices At The Intersection: A Listening Guide Analysis Of Campus-Based Advocates Navigating Institutional Policies On Sexual Violence, Arely Zaragoza-Hernandez

Open Access Theses & Dissertations

Campus-based advocates occupy a critical yet complex position within higher education institutions (Nightingale, 2023; Wood et al., 2021), serving as frontline support for students while simultaneously navigating the mandates, policies, and bureaucratic structures that define their roles. This research study explores the lived experiences of advocates working in university settings, focusing on how institutional mandates from compliance requirements to administrative expectations shape, constrain, and sometimes empower their daily practices. Using a qualitative, phenomenological approach, the study centers the voices of campus-based advocates in Texas to illuminate the tensions between their professional commitments to student-centered care and the institutional imperatives of …

Acute Effects Of Lactate Infusion On Metabolism, Ad Biomarkers, And Cognition, Riley E. Kemna, Paul J. Kueck, Anneka Blankenship, Casey S. John, Chelsea N. Johnson, Zachary D. Green, Hana Mayfield, Lauren Yoksh, Lance Johnson, John P. Thyfault, Jonathan D. Mahnken, Benjamin F. Miller, Jill K. Morris

Physiology Faculty Publications

BACKGROUND: Impaired cerebral glucose metabolism is a hallmark of Alzheimer's disease (AD). Lactate is an alternative brain fuel; however, whole-body lactate metabolism has not been measured in AD. METHODS: The Lactate for Energy and Neurocognition Trial (NCT05207397) was a single-arm trial that enrolled 24 cognitively healthy (CH) older adults and 12 cognitively impaired (CI) participants. Subjects underwent a stable isotope lactate infusion to evaluate lactate metabolism, cognitive testing, and blood biomarker analyses. pTau217, brain-derived tau (BD-tau), pTau181, glial fibrillary acidic protein (GFAP), neurofilament light chain (NfL), total tau, and brain-derived neurotrophic factor (BDNF) were analyzed by Simoa HD-X (Quanterix). RESULTS: …

Inhibition Of Astrocyte Signaling Leads To Sex-Specific Changes In Microglia Phenotypes In A Diet-Based Model Of Cerebral Small Vessel Disease, Jenna L. Gollihue, Khine Zin Aung, Colin B. Rogers, Leopoldine B. Galopin, Nicholas A. Wright, Pradoldej Sompol, Erica M. Weekman, Yuriko Katsumata, Josh M. Morganti, Christopher M. Norris

Physiology Faculty Publications

Hyperhomocysteinemia (HHcy)-inducing diets recapitulate cerebral small vessel disease phenotypes in mice including cerebrovascular pathology/dysfunction, neuroinflammation, synaptic deficits, and cognitive decline. We recently showed that astrocyte signaling through calcineurin(CN)/nuclear factor of activated T cells (NFATs) plays a causative role in these phenotypes. Here, we assessed the impact of astrocytic signaling on microglia, which set the inflammatory tone in brain. Seven-to-eight-week-old male and female C57BL/6 J mice received intrahippocampal injections of adeno-associated virus (AAV) expressing EGFP (AAV2/5-Gfa2-EGFP) or AAV expressing the NFAT inhibitor VIVIT (i.e., AAV2/5-Gfa2-VIVIT-EGFP). Mice were then fed with control chow (CT) or B-vitamin-deficient chow for 12 weeks to induce …

Mirabegron Treatment Reduces Myofibroblasts And Cxcr2 Expression In Adipose Tissue In Obesity, Brian S. Finlin, Hasiyet Memetimin, Philip M. Westgate, Jin Chen, Esther E. Dupont-Versteegden, Philip A. Kern

Physiology Faculty Publications

Background: Treatment with the β3-adrenergic receptor (AR) agonist mirabegron improves insulin sensitivity β, cell function, and glucose tolerance in individuals with obesity, without weight loss or a change in brown adipose tissue (BAT) (Finlin et al, J Clin Invest. 2020 May 1;130(5):2319-2331). Furthermore, mirabegron treatment increased protein expression of beige adipose markers and the number of anti-inflammatory macrophages, and changed the expression of genes involved in fibrosis and tissue remodeling in subcutaneous white adipose tissue (SC WAT). Methods: We utilized RNA seq and enrichment analysis to identify biological pathways changed by mirabegron treatment in SC WAT thigh biopsies. We verified …

Clinical Decision Support For Pharmacologic Management Of Treatment-Resistant Depression With Augmented Large Language Models, Martha Sajatovic

Faculty Scholarship

Background: We evaluated whether a large language model could assist in selecting psychopharmacological treatments for adults with treatment-resistant depression. Methods: We generated 20 clinical vignettes reflecting treatment-resistant depression among adults based on distributions drawn from electronic health records. Each vignette was evaluated by 2 expert psychopharmacologists to determine and rank the 5 best next-step pharmacologic interventions, as well as contraindicated or poor next-step treatments. Vignettes were then presented in random order, permuting gender and race, to a large language model (Qwen 2.5:7B), augmented with a synopsis of published treatment guidelines. Model output was compared to expert rankings, as well as …

Potential And Low-Cost Football Talents For Uae Clubs Based On Data-Driven Analysis, Rashid Alqemzi

Theses

The use of data analytics in professional football has changed the way clubs view, value and invest in players. Meanwhile, elite European teams have consistently exploited the data-driven strategies that offer them a competitive edge, but in up-and-coming football markets, such as the UAE Pro League, nepotism has remained strong, with reputation-based and agentdriven recruitment being the transformation choice of even the biggest profile moves. This work explores whether predictive analytics can discover hidden high-quality football talent from ”hotbeds” of talent including Brazil and Argentina, in a way that matches the economic capabilities of clubs in the UAE, given the …

Strainx : A Technical Exploration Of Cinema4d'S Native Particle System In An Experimental Title Sequence, Farah Ahmad

Theses

This paper presents a practice-based investigation into the capabilities of Cinema 4D’s native particle system through the creation of an experimental title sequence titled StrainX. The project was conceived as a technical and aesthetic exploration, prioritizing abstraction, form, and motion over narrative content. Through iterative testing and simulation, the study examines how Cinema 4D’s built-in particle, geometry, and material tools can generate visually complex results without relying on third-party plugins, such as X-Particles. All assets, simulations, and textures were developed natively within Cinema 4D, allowing for a unified and efficient workflow. The methodology emphasizes procedural experimentation, simulation caching using Alembic …

Biodegradation Of Common Post-Consumer Plastic, Laura Krebs

Theses

Plastic waste is widespread throughout the Laurentian Great Lakes watershed. During environmental exposure, plastic undergoes abiotic and biotic degradation. The presence of novel plastic substrates coupled with rapid microbial turnover may lead to selection for or evolution of metabolic pathways and enzymes with enhanced biodegradation capabilities. This study investigated the biodegradation potential of bacteria isolated from debris accumulation hotspots - stormwater retention ponds, storm drains, and tributaries - in the Lake Ontario watershed within Rochester, New York. Bacterial isolates were exposed to commonly littered plastics for 240 d: cellulose acetate from smoked cigarette filters, high-density polyethylene from takeout shopping bags, …

Asymptote, Aybuke Yilmazer

Theses

Asymptote is a short film that is an expression of mankind’s emotional consumption through confusing love with sex. This film is a frame-by-frame hand-drawn animation that combines digital and analog techniques. It was produced between August 2024 and April 2025, and its music was re-produced in August 2025. This paper is a written declaration of the motives and intentions of the director, Aybuke Yilmazer, in making this film, and analyzes the filmmaking process. The text of the thesis is in third person except for the self-evaluation section.

Symbiotic Furniture Design: A Single Chair Integrating Epiphytes With Taiwanese Window Grille Structures, Kuan-Wen Lin

Theses

Plants have been integrated into residential and interior environments for centuries, yet research on the psychological effects and interactive potential of plants in furniture design is still relatively limited. Traditional planting methods, such as individual potted plants or large green walls, often require additional floor or wall space, which is not ideal for environments with limited living space. Therefore, this study investigates how plants can be integrated into furniture design to enhance the effect of interior greenery and user engagement, and to redefine the relationship between furniture and the natural environment. This research explores a furniture design that incorporates epiphytic …

Defaking Deepfakes: Designing And Evaluating Ai-Powered Digital Media, Saniat Javid Sohrawardi

Theses

The rapid spread of generative AI has revolutionized media production, creating new challenges for information integrity as convincing deepfakes proliferate. Journalists play a critical role in upholding credible public information, yet existing deepfake detection technologies often overlook their specific workflows and requirements. This dissertation addresses these needs by identifying what journalists require from detection tools and evaluating usability in realistic scenarios through the following works: Journalists' Needs and Tool Design: Through qualitative user studies, we uncover journalists' preferences for tools that provide transparent, explainable evidence and context-aware analysis integrated into news verification routines. These insights drive the design of DeFake, …

Desiccation Tolerance In Tetradesmus Green Algae, Kristen Patten

Theses

In the face of the global climate change threat, understanding the adaptations that organisms have evolved to handle environmental variation is of particular interest to scientists. With climate change impacting global water availability and increasing the risk of drought for many traditional agricultural areas in the United States (IPCC, 2014, IPCC, 2021, and Kuwayama et al., 2019), desiccation tolerance in vegetative states is one adaptation that is currently receiving a lot of attention. Green algae are particularly useful organisms for understanding this adaptation due to their ubiquity across environments, which has given rise to independently evolved organisms displaying different levels …

Gender Considerations When Examining The Association Between Military Sexual Trauma And Chronic Pain Conditions, Lily Sarfaraz

Dissertations

Military sexual trauma (MST) amongst veterans is a growing concern for the Veterans Health Administration (VHA). Veterans who screen positive for MST receive VHA care for any injuries caused as a result of MST, psychological or physical, free of charge. MST is difficult to track, can result in long-term disability, and the burden of illness varies across genders. Current data highlights discrepancies seen across the literature of actual detected MST cases, with rates varying vastly. Efforts to track prevalence of MST identify additional concerns over the various comorbidities seen in MST cases. When examining symptom sequalae of MST, there is …

Margalefidinium Polykrikoides Group Iii Va, Usa Strain Growth And Sac-Like Pellicle Cyst Dynamics, Eduardo Pérez Vega

OES Theses and Dissertations

Margalefidinium polykrikoides is a harmful cosmopolitan dinoflagellate that blooms in coastal waters. The effect of temperature and salinity on the growth of M. polykrikoides VA strain was examined using microscopy and growth models. M. polykrikoides Group III VA strain grew better at warmer temperatures and lower salinities than M. polykrikoides Group III NY strain, Group I Korea strain, and Japan strain (unknown group). Modelers need to use the temperature and salinity growth responses from M. polykrikoides Group III VA strain to better simulate and predict M. polykrikoides blooms in the Chesapeake Bay.

Dinoflagellates produce cysts as a strategy to withstand …

Monte Carlo Simulations Of Geometric Deformation Of Harrison-Anderson-Mick Applicators Used In Intraoperative Radiation Therapy, Benjamin Insley, Brett Bocian, Sam Beddar, Reza Reiazi, Patrick James Jensen, Rachael M Martin-Paulpeter, Joshua Scott Niedzielski, David Flint, Gabriel Oliveira Sawakuchi, Luis Augusto Perles

Faculty, Staff and Student Publications

Background: The Harrison-Anderson-Mick (HAM) applicator is a high-dose-rate intraoperative radiotherapy (HDR-IORT) applicator used to position Ir-192 brachytherapy sources over surgically-accessed tumor volumes or post-resection tumor beds. Because of the lack of a 3D imaging system, dwell times are optimized pre-surgery by a TG-43-based treatment planning system (TPS) that assumes a perfectly flat applicator surface surrounded by an infinite water phantom. These assumed conditions are disparate from typical treatment conditions, especially in the pelvic regions, which often involve uneven patient surfaces and superficial irradiations with little to no backscatter material.

Purpose: Develop and validate a Monte Carlo (MC) model of HDR-IORT …

Evaluating A Blood Warming Device For Packed Red Blood Cell Transfusions To Decrease Hypothermia In Very Preterm Infants: A Randomised Control Trial Protocol, Kayla C. Everhart Phd, Rn, Michael D. Wirth Msph, Phd, Face, Victor N. Iskersky, Robin B. Dail Phd, Rn, Faan

Publications

Background: This protocol describes a study to test a commercial blood warming device in warming blood transfusions to prevent hypothermia associated with packed red blood cell (PRBC) transfusions in very preterm infants. Objectives: Very preterm infants receiving blood transfusions warmed by a commercial blood warmer will have less central body hypothermia post transfusion (abdominal temperatures < 36.5°C) and/or a higher mean abdominal temperature for the hour after the transfusion is complete compared to those receiving blood transfusions by standard of care. This trial has been registered with Clinicaltrials.gov (trial number NCT05170633). Methods: In this trial, which is funded by The Gerber Foundation and adhered to the SPIRIT2013 checklist, 140 very preterm infants with an order to receive PRBCs will be randomised into a standard of care group to receive one PRBC transfusion over 3 h with blood in a syringe at room temperature or into the intervention group to receive one PRBC transfusion over 3 h using a Hotline® Blood and Fluid Warmer. Abdominal skin temperatures will be measured every minute through a blood transfusion using a skin thermistor and datalogger. Data analysis: Descriptive statistics will be computed for each group to compare demographics and all infant pre-, during, and post-transfusion body temperatures. A student t-test will be used to compare the groups on mean post-transfusion temperatures as a primary outcome variable. To examine thermal change over time between the two groups, linear mixed models with a random intercept will be utilised. Results: This trial began in January 2022, in a South Carolina neonatal intensive care unit and is ongoing. Discussion: This RCT will determine if warming PRBC transfusions will prevent hypothermia in preterm infants during and after blood transfusions. Results from this trial will be used to design national standards for blood transfusions in preterm infants to decrease morbidity and mortality.

Investigating The Origins Of Symbiosis Using A Novel Mycorrhizal Interaction In Pasque Flowers (Pulsatilla Patens), Savannah Lane Draud

Electronic Theses and Dissertations

Mycorrhizal fungi form mutualistic symbioses with most land plants, occurring primarily as arbuscular mycorrhizal (AM) fungi or ectomycorrhizal (ECM) fungi. Pulsatilla patens, an herbaceous perennial widely distributed across Eurasia and North America, belongs to a plant family historically known only to form AM associations. However, recent discoveries of ECM P. patens individuals in northeastern Poland raise questions about the mycorrhizal status and evolutionary trajectory of this species. Because ECM formation in this lineage is likely evolutionarily recent, P. patens offers a valuable system for investigating the ecological and historical factors that facilitate the emergence of ECM symbiosis.

Chapter 1 assessed …

Rewarding Disruptions In Tamsyn Muir's The Locked Tomb, Elijah Moss

Undergraduate Honors Capstone Projects

Tamsyn Muir, author of The Locked Tomb, says she thinks of Biblical allusions in the same way she thinks of alluding to digital folklore. This research paper investigates the veracity of this claim, using the dual lenses of literary analysis and digital folklore studies to ultimately conclude that the broad range of intertextual references in The Locked Tomb exemplifies a broader strategy of ambivalence (e.g., in her tone) that disrupts the readerly experience. With these ruptures, Muir creates space away from the typical expectations that may limit the range of themes historically found in genre fiction. Therefore, her tonal …

News From Hope College, Volume 57.2: Winter 2025, Hope College

News from Hope College

No abstract provided.

Bundled For The Beyond: Material Analysis Of A Canine Mummy Bundle For Contextualizing And Conserving Legacy Objects, Sascha Anna-Rose Baldauf

Undergraduate Honors Capstone Projects

Legacy collections¹ and objects with little context are often untapped opportunities for both museums and museum studies students. The purpose of this study is to display how students can utilize object research to engage in experiential learning and develop their skillset in scientific methodologies and disseminating findings, while also offering museums the opportunity to develop relationships with research facilities and benefit from student research findings. This study demonstrates the utility of student research by investigating an Egyptian canid mummy bundle, dating to the Late Period² or early Ptolemaic Period³, received by the Museum of Anthropology at …

Public Health Trade-Offs During A Pandemic: Mobility Decline And Hypertension Risk In India, Arpita Khanna, Tomoki Fujii

Research Collection School Of Economics

The Covid pandemic dramatically altered patterns of daily life, particularly through widespread declines in mobility due to lockdowns and social distancing measures. While the economic and epidemiological effects of these restrictions have been widely studied, their broader health implications remain underexplored. This paper investigates the relationship between mobility decline during the Covid pandemic and hypertension outcomes in India. Leveraging district-level variation in mobility from Google Community Mobility Reports and health data from the 2019–2021 Indian Demographic and Health Survey (DHS), we find that a 1 percentage point reduction in mobility over the 30 days prior to interview is associated with …

Pathways To Leadership: How Meaningful Experiences Shape Motivation In 4-H Teen Leaders, Trang Ta, Steven Krauss

Journal of Youth Development

Youth leadership development is crucial for empowering young people to thrive and contribute to society. Research on 4-H has demonstrated significant leadership growth among program participants, with effects persisting into emerging adulthood. However, little is known about what specific experiences shape 4-H youth’s motivation to lead. This qualitative study explores meaningful experiences—vividly remembered moments with positive and enduring effects—that nurture leadership motivation among 4-H teen leaders. Seven emerging adults aged 18-21 with extensive leadership experience in Missouri 4-H programs participated in semi-structured, in-depth interviews. Using a thematic analysis approach, we identified two categories of meaningful experiences, namely transformative moments and …

Evaluation Of Computed Tomography Radiation Dose Metrics In Saudi Arabia: Comparison To National And International Diagnostic Reference Levels, Mohammad Esmael Alsulimane

Saudi Medical Journal

ABSTRACT This review evaluates CT radiation dose levels in Saudi Arabia relative to national Diagnostic Reference Levels (DRLs) established by the Kingdom of Saudi Arabia (KSA) and the United Kingdom (UK). A total of 31 studies published between 2015 and 2025 were reviewed, covering adult and pediatric CT protocols for head, chest, abdomen, pelvis, and multiphase scans. The review highlights variations in the volumetric computed tomographic dose index (CTDIvol), and the dose-length product (DLP) values among different institutions in KSA. For adult protocols, fifteen studies reported dose metric values below the KSA NDRLs, while 8 studies reported values below the …