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Articles 10771 - 10800 of 31500
Full-Text Articles in Entire DC Network
Black Oystercatcher Haematopus Bachmani Productivity In California And Oregon And The Effects Of Nest Site And Environmental Covariates, Anna Weinstein, Joseph Liebezeit, Daniel Orr, Ryan D. Carle, Timothy D. Meehan
Black Oystercatcher Haematopus Bachmani Productivity In California And Oregon And The Effects Of Nest Site And Environmental Covariates, Anna Weinstein, Joseph Liebezeit, Daniel Orr, Ryan D. Carle, Timothy D. Meehan
Marine Ornithology
Black Oystercatcher Haematopus bachmani is a species of conservation concern due to its small global population, reliance on somewhat restricted intertidal habitat, and vulnerability to climate change impacts. Concern for this species, along with a lack of demographic information from the southern portion of its range, gave rise to a community science project to monitor pair productivity, nest characteristics, and nest disturbance from 2012 through 2022 across three study regions in Oregon and California. A clear spatial gradient existed in productivity, with relatively low values in the southern region (0.37 fledged young per pair per year, 95% confidence interval [CI] …
Assessment Of Tufted Puffin Fratercula Cirrhata Diet Using Dna Metabarcoding, Thomas P. Good, Andrew O. Shelton, Abigail H. Wells, Ana Ramón-Laca, Ramon Gallego, Peter J. Hodum, Scott F. Pearson
Assessment Of Tufted Puffin Fratercula Cirrhata Diet Using Dna Metabarcoding, Thomas P. Good, Andrew O. Shelton, Abigail H. Wells, Ana Ramón-Laca, Ramon Gallego, Peter J. Hodum, Scott F. Pearson
Marine Ornithology
Investigating trophic relationships can be critical for understanding relationships between marine predators and their prey. DNA analysis of feces is used increasingly as a non-invasive method to uncover seabird dietary patterns across space and time. Tufted Puffins Fratercula cirrhata are listed as Endangered in the state of Washington (WA), USA, and reduced prey availability is thought to be a key factor in the species’ decline. Recent information on Tufted Puffin diet is lacking, and present opportunities for direct diet observation are limited. We conducted a pilot study to characterize Tufted Puffin diet on Destruction Island, WA, in 2019 using DNA …
Oviposition Lag In Nazca Boobies Sula Granti, Leslie D. Clifford, David J. Anderson
Oviposition Lag In Nazca Boobies Sula Granti, Leslie D. Clifford, David J. Anderson
Marine Ornithology
Egg formation in well-studied poultry includes rapid yolk deposition (RYD), which completes the formation of the ovum, then ovulation and uptake of the ovum by the oviduct to complete egg formation. Roughly a day passes between the end of RYD and oviposition in these species. In other species, notably seabirds, egg formation is paused at the end of RYD for days or even more than a week. The phylogenetic coverage of data on this “oviposition lag” is patchy. We present the first estimate of oviposition lag for the Sulidae (4 d) and only the second estimate for the Suliformes.
The Distribution, Abundance, And Habitat Use Of Wintering Iceland Gulls Larus Glaucoides Thayeri In Northern California And Comparisons With Other Larus Gulls., Scott S. Moorhouse
The Distribution, Abundance, And Habitat Use Of Wintering Iceland Gulls Larus Glaucoides Thayeri In Northern California And Comparisons With Other Larus Gulls., Scott S. Moorhouse
Marine Ornithology
Recent studies have provided significant insights, but relatively limited information is available on many aspects of the ecology and behavior of the Arctic-breeding Iceland Gull Larus glaucoides. In this study, the distribution, abundance, and habitat use of wintering Iceland Gulls Larus glaucoides thayeri in Northern California, USA, were analyzed in 30 habitat types over three winters. A total of 6012 individuals was recorded, contributing 2.3% of the total Larus gulls recorded. The mean number of Iceland Gulls recorded per count was highest in sanitary landfills located in the Inner Coast Range, Central Valley, Inner Coast, and San Francisco Bay Plain …
Life-History Traits And Divergence In Population Trends Of Two North Pacific Auks: Rhinoceros Auklet Cerorhinca Monocerata And Tufted Puffin Fratercula Cirrhata, J. Mark Hipfner
Marine Ornithology
Population trends in two closely related and ecologically similar North Pacific auks, the Rhinoceros Auklet Cerorhinca monocerata and Tufted Puffin Fratercula cirrhata, have diverged over recent decades: stable in the former, declining in the latter. I propose that differences between the two species in a broad suite of interrelated morphological, demographic, behavioral, and physiological life-history traits could explain their differing responses to recent environmental conditions.
A Regionally Significant Population Of White-Tailed Tropicbirds Phaethon Lepturus On Kurehdhoo (Lhaviyani Atoll), Republic Of The Maldives, James C. Russell, Sebastian Steibl, Guy M.W. Stevens
A Regionally Significant Population Of White-Tailed Tropicbirds Phaethon Lepturus On Kurehdhoo (Lhaviyani Atoll), Republic Of The Maldives, James C. Russell, Sebastian Steibl, Guy M.W. Stevens
Marine Ornithology
Coral atoll islands are important breeding sites for tropical seabird species, but on many of these islands, disturbances from humans and introduced mammals have extirpated breeding colonies. In the Republic of the Maldives (Indian Ocean), little published information exists on the location and extent of seabird breeding colonies. Here, we document what appears to be a regionally significant breeding site for White-tailed Tropicbirds Phaethon lepturus on Kurehdhoo, the northern-most inhabited island of Lhaviyani Atoll in the central Maldives. We systematically surveyed the entire island on two separate occasions, six months apart, to count the number of White-tailed Tropicbird nests, their …
Notes On The Birds Of Schirmacher Oasis, Dronning Maud Land, East Antarctica., Peter G. Ryan
Notes On The Birds Of Schirmacher Oasis, Dronning Maud Land, East Antarctica., Peter G. Ryan
Marine Ornithology
The Schirmacher Oasis in central Dronning Maud Land, East Antarctica, has a long history of ornithological observations and is one of the few areas in East Antarctica experiencing a warming climate. Snow Petrels Pagodroma nivea and Wilson’s Storm Petrels Oceanites oceanicus were reported to breed at Schirmacher Oasis in the past, but currently the only confirmed regular breeding species is the South Polar Skua Stercorarius maccormicki. This note summarises observations at the Oasis in November and December 2023. Wilson’s Storm Petrels were the most abundant bird, breeding at several sites along the northern scarp in December. Pairs of Adelie Penguins …
Masthead, Pacific Seabird Group
Contents, Pacific Seabird Group
Inside Front Cover, Pacific Seabird Group
Evicted From The Outside, Colleen Cosgriff
Evicted From The Outside, Colleen Cosgriff
Maine Law Review
In recent years, Maine has seen an increase in the number of people experiencing homelessness who are living outdoors. People living in these circumstances often shelter together in groups of tents or other temporary structures commonly referred to as “encampments.”As municipalities across Maine struggled with how best to respond to the increase in encampments across the state, the United States Supreme Court decided City of Grants Pass v. Johnson, a case originating in Grants Pass, Oregon. Grants Pass wanted to issue civil and criminal fines to people for sleeping outdoors, even when no alternative shelter was available. The city ultimately …
Hydro-Morphodynamic Characterization Of Areas With Potential For Nearshore Wind Farms Using Computational Modeling, Nahome Paz Azevedo Dos Santos, André Martelo, Juliana Costi
Hydro-Morphodynamic Characterization Of Areas With Potential For Nearshore Wind Farms Using Computational Modeling, Nahome Paz Azevedo Dos Santos, André Martelo, Juliana Costi
Seatific Journal
The hydrodynamic and morphodynamic characterization of areas with potential for the installation of wind farms in Lagoa dos Patos is necessary as part of the planning for future wind energy projects. The aim of this study is to use computational modeling to describe the hydrodynamics of the lagoon system, focusing on areas with the greatest potential for wind farm installation. The three-dimensional hydrodynamic module TELEMAC-3D and the morphodynamic module SISYPHE, both from the Open TELEMAC-MASCARET system, were used in coupling for numerical simulations covering the period from 2005 to 2009. The analyses considered parameters that force the lagoon system, such …
Race-Neutral Pft: Implementing Patient Activation Measure Following Race- Neutral Pulmonary Function Testing, Ann M. Way
Race-Neutral Pft: Implementing Patient Activation Measure Following Race- Neutral Pulmonary Function Testing, Ann M. Way
Dissertations
Abstract
Background
The introduction of a race-neutral equation to predict normal values on pulmonary function testing (PFT) has led to changes in the diagnosis of pulmonary diseases previously diagnosed using a race specific equation. Minority patients appear to be most likely impacted by this change, potentially impacting how they self-manage their pulmonary health. Patient self-management can be measured and assessed by using the Patient Activation Measure (PAM) tool.
Purpose
The purpose of this project is to assess patient activation levels amongst minority patients with a new or changed pulmonary diagnosis to identify a possible need for interventions to improve patient …
Depression Screening In The Adult Inpatient Oncology Population, Erica Bizub
Depression Screening In The Adult Inpatient Oncology Population, Erica Bizub
Dissertations
Routinely screening oncology patients for depression is recommended due to the higher rates of depression in this population as well as the negative impact of untreated depression. The goal of this QI project is to implement a standard depression screening protocol for patients admitted to this hospital, where there is currently no standard screening protocol. There were 110 patients included in this study, and 67 (60.91%) of patients received a PHQ-9 screening. Of the patients who completed the PHQ-9, 21 (19.09%) had a positive screening, which was a score of seven or higher. While there were high rates of provider …
A Program Evaluation: Insomnia In Adults With Major Depression Disorder With Cognitive Behavioral Therapy For Insomnia (Cbt-I), Taylor E. Wilson
A Program Evaluation: Insomnia In Adults With Major Depression Disorder With Cognitive Behavioral Therapy For Insomnia (Cbt-I), Taylor E. Wilson
Dissertations
Abstract
Problem: In 2023, Compass Health Network (CHN) data analysts found that 91% of CHN patients in the United States who have died by suicide reported problems with sleep, which was the highest risk factor for suicide. The objective of this program evaluation project is to evaluate CHN CBT-I effectiveness on patients ‘suicidality by improving their insomnia.
Methods: CHN quality improvement (QI) project used a quantitative method pre/post score design. Quantitative aggregate data was collected on the participants who met the inclusion criteria via retrospective and prospective chart review prior to CHN implementing the intervention. CBT-I clinicians collected PHQ-9, Columbia-Suicide …
Preparation Of Human Asprosin Nanobodies Based On A Natural Nanobody Phage Display Library, Yun Liang, Yi Huang, Qinghua He
Preparation Of Human Asprosin Nanobodies Based On A Natural Nanobody Phage Display Library, Yun Liang, Yi Huang, Qinghua He
Journal of Food Science and Biotechnology
[Objective ] Asprosin (ASP ),an adipokine produced from fibrillin- 1 (FBN 1) cleavage,is closely linked to type 2 diabetes mellitus and obesity when its level is abnormal,demonstrating significant effect on human health.Therefore,monitoring the ASP level is crucial.The author aims to screen the nanobodies specifically binding to ASP via phage display technology,offering a new antibody component for the research on ASP.[Method ] The antigenic epitopes of ASP were predicted by the online tools ABCpred and NovoPro,and then coupled with bovine serum albumin (BSA ) to form the coating antigen (ASP-Epitope-BSA ).After five rounds of bio-panning,the nanobodies specifically binding to ASP were …
Modulatory Effects Of Flavonoids On Il- 4/Il-13 In Atopic Dermatitis And Their Structure-Activity Relationships, Yueyuan Wang, Jing Liu, Chen Li, Fengfeng Chen, Cheng Yang, Bingtian Zhao
Modulatory Effects Of Flavonoids On Il- 4/Il-13 In Atopic Dermatitis And Their Structure-Activity Relationships, Yueyuan Wang, Jing Liu, Chen Li, Fengfeng Chen, Cheng Yang, Bingtian Zhao
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the ameliorating effects of flavonoids on atopic dermatitis (AD) and their structure-activity relationships.[Method ] Flavonoids were selected through network pharmacology with interleukin- 4 (IL-4) and interleukin- 13 (IL-13) as key targets.The immunoglobulin E (IgE)-induced RBL- 2H3 cell model was employed to evaluate the effects of flavonoids on inflammatory factors,degranulation activities,and calcium ion influx,and the structure-activity relationships were analyzed.Finally,molecular docking was utilized to assist in exploring the action mechanism.[Result ] All of the six flavonoids (luteolin,apigenin,chrysin,quercetin,kaempferol,and fisetin ) screened by network pharmacology had significant inhibitory effects on the expression of IL- 4,IL- 13,tumor necrosis …
Structural And Solution Property Characterization Of Oat Β-Glucans, Hongli Zhang, Yaning Shi, Ming Miao
Structural And Solution Property Characterization Of Oat Β-Glucans, Hongli Zhang, Yaning Shi, Ming Miao
Journal of Food Science and Biotechnology
[Objective ] This study aims to characterize the structure and investigate the solution properties of oat β-glucan (OBG ).[Method ] Two soluble OBGs with different weigh-average molecular weights (Mw),number-average molar weights (Mn),and peak molecular weights (Mp) were prepared by hot water extraction and ethanol classification.The structures and solution properties were characterized by Fourier-transform infrared spectroscopy (FT-IR ),nuclear magnetic resonance (NMR ),X-ray diffraction (XRD ),scanning electron microscopy (SEM ),high performance size exclusion chromatography coupled with multi-angle laser light scattering (HPSEC-MALLS ),and rheology.[Result ] OBG- 1 and OBG- 2 showed the Mw of 80.73×104 g/mol and 8.05×104 g/mol,the yields of (4.86±0.55)% and …
Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots, Junjie Sun, Xiaoxia Liu, Ziyi Chen, Yinlai Li, Lu Zhai, Qinzhou Chen, Renyao Jin
Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots, Junjie Sun, Xiaoxia Liu, Ziyi Chen, Yinlai Li, Lu Zhai, Qinzhou Chen, Renyao Jin
Journal of Food Science and Biotechnology
[Objective ] This study aims to establish a rapid immunoassay method for the quantitative detection of eugenol.[Method ] The eugenol monoclonal antibody was conjugated to the surface of quantum dots by the 1-ethyl- 3- (3-dimethylaminopropyl ) carbodiimide (EDC ) and N-hydroxy-succinamide (NHS ) (EDC-NHS ) coupling method,with quantum dots as fluorescent markers.Bovine serum albumin (BSA ) was used for blocking,and fluorescence microscopy,agarose gel electrophoresis,and a microplate reader were used for identification.Furthermore,the established method was compared with high performance liquid chromatography (HPLC ).[Result ] The optimal synthesis conditions were as follows:quantum dot activation time of 30 min,conjugation pH with 8.0,antibody addition …
Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch, Lei Lei, Wenjun Fu, Lingling Yang, Haibo Liu, Daxi Sang, Qing Wang
Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch, Lei Lei, Wenjun Fu, Lingling Yang, Haibo Liu, Daxi Sang, Qing Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the properties of chestnut starch.[Method ] Tea polysaccharides were added to chestnut starch in different proportions,and the effects of tea polysaccharides on the water-holding capacity,digestive properties,textural properties,and other properties of chestnut starch were investigated.[Result ] The water-holding and oil-holding properties of chestnut starch were the largest when tea polysaccharides were added at 1% and then gradually decreased with the increase in the proportion of tea polysaccharides added.The transparency of chestnut starch gradually increased when tea polysaccharides were added from 1% to 8% and decreased significantly when tea polysaccharides were added at 16%.Adding tea …
A Study Of Machine Learning Techniques In Solving Biochemical And Chemical Problems, Kenneth Micheal Plackowski
A Study Of Machine Learning Techniques In Solving Biochemical And Chemical Problems, Kenneth Micheal Plackowski
Chemistry and Chemical Biology ETDs
Data-driven approaches to solving problems in biology and chemistry require utilization of reliable techniques and machine learning algorithms are the modern reliable approach. This work presents three problems that involve use of supervised learning techniques when classification is the goal and unsupervised learning techniques when global data representation is the goal.
In the first problem, we demonstrate the use of unsupervised clustering techniques, self-organizing maps and K-means, to ascertain analyte detection capabilities of carbon nitride dots. In the second problem, we add scalability features to a functional group classification model applied to infrared data and evaluate its ability to inform …
Nanoporous Gold Nanoparticles And Model Membrane Systems Applied To The Study Of Lipopolysaccharide (Lps), Dhanbir Lingden
Nanoporous Gold Nanoparticles And Model Membrane Systems Applied To The Study Of Lipopolysaccharide (Lps), Dhanbir Lingden
Dissertations
Lipopolysaccharide (LPS), a key structural component of Gram-negative bacteria, is a major trigger of sepsis and septic shock, the systemic inflammatory conditions responsible for millions of deaths annually. Even at concentrations as low as 1 ng/mL, LPS can activate the immune system and initiate inflammatory cascades. According to the Global Burden of Disease Study 2017, sepsis accounted for 48.9 million cases globally, with 11 million deaths, emphasizing the urgent need for improved diagnostic and therapeutic strategies.
Accurate and rapid detection of LPS is vital for early intervention and improved clinical outcomes. While traditional detection methods are still widely used, modern …
Screen Foundations: How Pediatric Nurse Practitioners Can Implement ‘Family Media Use Plans’ In Primary Care To Reduce Pediatric Behavior Concerns, Katie Anderson
Dissertations
Abstract for:
Screen Foundations: How Pediatric Nurse Practitioners Can Implement ‘Family Media Use Plans’ in Primary Care to Reduce Pediatric Behavior Concerns
Problem: Parents of children 2-5 years-old increasingly reach out to PCPs for escalating behavior concerns, including when the child doesn’t listen well, is inattentive, having tantrums, meltdowns, hitting, yelling, etc. Referrals to psychiatry and/or play therapy result in long waits, or referrals not accepted. Coincidingly, toddler and preschool aged children continue to exceed daily screen time recommendations. This results in a gap of care for families with young children and externalizing behavior.
Methods: This quality improvement project addressed …
Research Progress In Thermo-Reversible Polysaccharide Gels, Dongxin Zhou, Ziao Zhang, Su Chen, Weimin Yang, Jiajun Sun, Shengzu Han, Anning Xie, Xiaoxiao Li, Yuxiang Bai
Research Progress In Thermo-Reversible Polysaccharide Gels, Dongxin Zhou, Ziao Zhang, Su Chen, Weimin Yang, Jiajun Sun, Shengzu Han, Anning Xie, Xiaoxiao Li, Yuxiang Bai
Journal of Food Science and Biotechnology
Thermo-reversible gels are widely applied in the processing of baking,meat,and snack products including cakes,candies,jelly,aspic,and steamed custard buns.Currently,the most effective thermo-reversible gel is gelatin,which can combine with water to form a transparent gel that melts when it is close to the temperature of the human oral cavity.However,gelatin is expensive.Therefore,researchers have turned their attention to polysaccharide gels,hoping to obtain more thermo-reversible food raw materials with different properties.The author reviews thermo-reversible polysaccharide gels from natural sources such as plants,algae,and microorganisms and those modified by physical,chemical,and enzymatic methods and summarizes the sources,structures,gelatinization mechanisms,and product applications of polysaccharide gels.It is expected to provide a reference …
Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases, Yixiao Shao, Chuang Huang, Chenjian Liu, Xiaoran Li
Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases, Yixiao Shao, Chuang Huang, Chenjian Liu, Xiaoran Li
Journal of Food Science and Biotechnology
The antioxidant properties of lactic acid bacteria are related to various probiotic activities,which can prevent and alleviate a variety of chronic diseases caused by oxidation in the host.The antioxidant mechanisms of lactic acid bacteria,including the action mechanisms of antioxidant enzymes and non-enzymatic antioxidants and regulation of host signaling pathways,were expounded.Furthermore,the application status of antioxidant properties of lactic acid bacteria in diseases of the nervous system,digestive system,and skin system were summarized.The aim is to explore how to enhance the antioxidant activity of lactic acid bacteria in oxidative stress-related diseases and give new insights into the development of related treatment strategies.
Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38, Cong Gao, Weijia Yuan, Chao Ye, Jia Liu, Liming Liu
Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38, Cong Gao, Weijia Yuan, Chao Ye, Jia Liu, Liming Liu
Journal of Food Science and Biotechnology
[Objective ] A genome-scale metabolic model (GSMM ) of the high L-malic acid-producing strain Aspergillus oryzae (A.oryzae ) FMME-S- 38 was constructed and analyzed.[Method ] The new GSMM iJW1333 was established for A.oryzae FMME-S- 38 through a two-step process of draft reconstruction and model refinement.[Result ] The model integrated 1 723 biochemical reactions,1 723 metabolites,and 1 333 functional genes,achieving the prediction accuracy of 94.6% for substrate metabolism and 92.0% for cell growth.Comparative genomic analysis revealed that mutations in the genes involved in the hexose monophosphate pathway (HMP ) and oxidative phosphorylation underpinned the enhanced L-malic acid production of A.oryzae FMME-S- …
Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins, Haibo Zhang, Yongjun Xia, Guangqiang Wang, Ning Peng, Zhixian Chen, Lianzhong Ai
Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins, Haibo Zhang, Yongjun Xia, Guangqiang Wang, Ning Peng, Zhixian Chen, Lianzhong Ai
Journal of Food Science and Biotechnology
[Objective ] The extraction efficiency of different methods and the effects of these methods on the composition of mannoproteins extracted from yeast cell walls were compared.Furthermore,the anti-inflammatory activities of the extracted mannoprotein were evaluated.[Method ] Three yeast mannoproteins (MP-W,MP-B,and MP-E ) were extracted via high-temperature water,alkali,and enzyme-assisted extraction methods and purified via ethanol precipitation.The identification of monosaccharide composition and amino acid composition,distribution of relative molecular mass,and Fourier transform infrared spectroscopy were employed to analyze the composition and chemical structures of yeast mannoproteins.A cell experiment was carried out to examine the anti-inflammatory activity.[Result ] The yields of yeast mannoproteins extracted by …
In Vitro Antioxidant And Anti-Inflammatory Activities Of Tea Flower Extract And Its Inhibitory Effect On Cell Aging, Shishuai Yu, Yining Hao, Bo Peng, Youyun Liu, Liping Qu
In Vitro Antioxidant And Anti-Inflammatory Activities Of Tea Flower Extract And Its Inhibitory Effect On Cell Aging, Shishuai Yu, Yining Hao, Bo Peng, Youyun Liu, Liping Qu
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the in vitro antioxidant and anti-inflammatory activities of Yunnan tea flower extract (TFE ) and its Inhibitory effect on cell aging.[Method ] The main active components in TFE were determined by ultraviolet (UV)-visible spectrophotometry and high performance liquid chromatography (HPLC ).The antioxidant and anti-inflammatory activities of TFE were determined by the free radical scavenging assay and UV-induced photoaging model of human keratinocytes (HaCaT ),respectively.After the aging model of fibroblasts was established,the aging level of cells was assessed by the β-galactosidase (β-gal) staining assay,and the expression of matrix metalloproteinase- 1 (MMP- 1),matrix metalloproteinase- 3 …
Synthesis Of N-Acetyl- Trans -4-Hydroxyproline Catalyzed By Acyltransferase, Chuanxiang Wei, Keru Chen, Yun Hu, Chao Sun, Rukang Xue, Fubao Sun, Jingfang Cheng
Synthesis Of N-Acetyl- Trans -4-Hydroxyproline Catalyzed By Acyltransferase, Chuanxiang Wei, Keru Chen, Yun Hu, Chao Sun, Rukang Xue, Fubao Sun, Jingfang Cheng
Journal of Food Science and Biotechnology
[Objective ] This study aims to establish the biosynthesis process of N-acetyl- trans -4-hydroxyproline (N-ATHOP ).[Method ] Escherichia coli (E.coli ) BL21 (DE3) was selected as the host to heterologously express the acyltransferase (MsAcT ) derived from Mycobacterium smegmatis.A semi-rational design was employed to construct the mutant MsAcT (S11C) capable of catalyzing the synthesis of N-ATHOP.[Result ] The catalytic reaction conditions of the mutant MsAcT (S11C) were optimized as pH 6.9,reaction temperature 49 ℃,initial trans -4-hydroxyproline (THOP ) concentration of 315.5 mmol/L,and vinyl acetate volume fraction of 10%.Under the optimized conditions,the yield of N-ATHOP synthesized under the catalysis of MsAcT …
Identification And Spoilage Capacity Analysis Of Main Spoilage Bacteria In Pickles, Li Zhu, Huimin Li, Liejiang Yuan, Cancan Peng, Bo Zhou, Sheng Song, Haizhi Wu, Mingyang Zhu, Fangmei Wang, Yu Liu, Yuqian Hu, Ge Li, Yiqing Sun, Yi Zhou
Identification And Spoilage Capacity Analysis Of Main Spoilage Bacteria In Pickles, Li Zhu, Huimin Li, Liejiang Yuan, Cancan Peng, Bo Zhou, Sheng Song, Haizhi Wu, Mingyang Zhu, Fangmei Wang, Yu Liu, Yuqian Hu, Ge Li, Yiqing Sun, Yi Zhou
Journal of Food Science and Biotechnology
[Objective ] This study aims to identify the main spoilage bacteria and evaluate their spoilage capacity in the spoilage process of pickles.[Method ] High-throughput sequencing was employed to analyze the microbial community dynamics in the samples of pickles at the early,middle,and late stages of spoilage.The key spoilage bacteria were isolated,purified,and then identified through a combination of colony morphology observation,biochemical analysis,and 16S rDNA/ITS sequencing.Re-inoculation experiments were carried out for further validation of the primary spoilage bacteria in pickles.[Result ] Acinetobacter and Bacillus were the dominant spoilage bacterial genera across the three spoilage stages.Notably,Bacillus exhibited rapid growth over time,with the relative abundance …