Digital Commons Network^™

Health Workers’ Perspectives On Family Medicine And Family Physicians In Karbala City, Iraq, Haneen Nadhim Jawad, Ali Abdulridha Abutiheen, Hasan Alwan Baiee, Salam Sahib Obiad

Hilla University College Journal For Medical Science

Background: The opinions and experiences of health workers can provide valuable insights into the current state of family medicine and its potential to improve healthcare services in any country, including Iraq.

Objectives: To identify health workers’ perceptions in Karbala Governorate regarding the role of family medicine and family physicians, and to explore their opinions on the performance of primary health care centers.

Materials and Methods: A cross-sectional survey was carried out from March through May 2024, involving health professionals from various health service settings in Karbala Governorate, Iraq. Data were collected through a structured, self-administered validated questionnaire covering demographic …

Study The Metabolic And Hormonal Changes In Obese And Non-Obese Women With Polycystic Overy Syndrome: Case-Control Study, Hawraa Shakir Issa, Hanaa Addai Ali

Hilla University College Journal For Medical Science

Background: Women with PCOS experience pathological symptoms like diabetes and insulin resistance (IR), obesity and being overweight, infertility, and irregular menstruation due to hormonal abnormalities.

Objectives: Study the association between metabolic and hormonal changes in PCOS.

Materials and methods: Forty-five Iraqi women with newly diagnosed PCOS, aged between 20–40 years, were divided into two groups X Obese Women with PCOS, Y Non-Obese PCOS. The samples were collected from private labs, Al-Sadr Teaching Hospital, and Al-Zahraa Hospital in the Najaf Governorate. Each was tested using an enzyme-linked immunosorbent assay (ELISA) for the subject’s reproductive hormones and by colorimetric methods used to …

Assessment Of Autonomic Dysfunction In Iraqi Patients With Parkinson’S Disease Using The Ssr, Ahmed Ayad Al-Janabi, Abdulnasir Hussin Ameer

Hilla University College Journal For Medical Science

Background: Parkinson’s disease (PD) is a progressive neurodegenerative disorder that affects both motor and non-motor functions, including the autonomic nervous system. Autonomic dysfunction, particularly involving sympathetic pathways, is a common but underdiagnosed feature in PD.

Objectives: This study aimed to assess autonomic dysfunction in Iraqi patients with Parkinson’s disease using the Sympathetic Skin Response (SSR) test as a non-invasive neurophysiological tool.

Methods: A case-control study was conducted at Ghazi Al-Hariri Surgical Teaching Hospital, involving 40 patients diagnosed with PD and 40 age-matched healthy controls. All participants underwent SSR testing to evaluate latency and amplitude in the hands and feet under …

Clinical Correlation Between Preoperative Hypocalcemia And Post-Thyroidectomy Hypocalcemia In Patients With Goiter, Dauda Bawa, Saleem Khan, Yasser M. Khalifa, Chinedu M. Mbalisigwe

Journal of King Abdulaziz University: Medical Sciences

Many patients undergoing thyroidectomy present with subclinical hypocalcemia preoperatively, the prevalence and significance of which remain unclear. This study aimed to determine the prevalence and impact of preoperative hypocalcemia in goiter patients undergoing thyroid surgery, a complication often encountered postoperatively. Out of the 255 patients examined, the study found a preoperative hypocalcemia rate of 20.4%, which rose to 26.1% following thyroidectomy, specifically peaking at 21.1% in instances of total thyroidectomy. Postoperative symptomatic hypocalcemia occurred in 8.9% with a mean serum calcium level of 1.87 mmol/L. In the linear regression analysis, a negative correlation was observed between thyroid procedures and overall …

Relationship Of Prolonged Exclusive Breastfeeding To Infant Anemia, Maryam M. Ahmed, Aveen S. Shareef, Farhad S. Armishty, Dunya P. Nizar, Ronahi Y. Ibrahim, Lina Y. Mohammed, Snor H. Husain, Berivan R. Rajab, Omar Khatab, Dalal A. Jameel, Amna A. Omar, Iman H. Yaseen, Hevy A. Ahmad

Journal of King Abdulaziz University: Medical Sciences

Background: Anemia can adversely affect cognitive development in infants and children, potentially leading to challenges in cognition, motor control, social interaction, and neurophysiological functioning. Full-term infants do not develop iron deficiency anemia within the first four months of life, as they are born with sufficient iron stores accumulated during their intrauterine life. These stores are usually adequate for four to six months of postnatal life. However, exclusive breastfeeding beyond six months without iron supplementation can increase the risk of iron deficiency anemia, as breast milk does not provide enough iron to meet the growing infant’s demand. The American Academy of …

Factors Contributing To Intestinal Parasitic Infestations In Children Attending Primary Private And Public Schools In Central Benghazi, Asmaa A. Alfarisi, Samira S. Garboui, Fawzeia H. Elmhalli, Maha A. Kattou, Suhaila Elabbar, Hajir A. Alabede, Zahrah A. Alawami

Journal of King Abdulaziz University: Medical Sciences

Background: Intestinal parasitic infections (IPIs) among school-aged children are a significant public health issue in many parts of the world, with high prevalence rates in many regions. The study aims to investigate the prevalence and associated risk factors of IPIs among school children.

Materials and Methods: A cross-sectional study was conducted among randomly selected primary school children aged 6 to 9 years in central Benghazi from April to September 2021. Stool samples were collected from 700 schoolchildren and analyzed using the wet-mount technique. Also, questionnaires were distributed, and general information on each child was recorded.

Results: The study revealed a …

Self-Prescription Of Beta-Blockers Among All Healthcare Students In Jeddah, Saudi Arabia: A Cross-Sectional Study, Duaa M. Bakhshwin, Lama A. Aljialni, Nada M. Bawajeeh, Rimaz T. Alanazi, Duaa M. Alshabani, Marah M. Alsulami, Rahaf A. Alothman, Reem H. Alnayeli, Dina A. Ajaj, Shahad H. Albar, Rowthanh H. Murshid

Journal of King Abdulaziz University: Medical Sciences

Objectives: We investigated the prevalence and implications of inappropriate self-prescription of beta-blockers among healthcare students in Jeddah, primarily driven by anxiety related to academic performance.

Method: An analytical, observational, cross-sectional design was employed, and a self-administered electronic questionnaire was distributed via social media channels after obtaining ethical approval. A total of 1,043 healthcare students were included from June to December 2023. Data were collected using a validated questionnaire that focused on demographics, academic performance (grade point average), beta-blocker usage, and awareness of potential side effects. Results: Among the 175 students who were taking beta blockers, 54.3% self-prescribed beta-blockers, predominantly propranolol, …

Enhancing Awareness Of Autoimmune Diseases In Schools: A Saudi Cross-Sectional Study To Educate And Empower Adolescents, Mohammed Nashawi, Jumanah Nassar, Lina Barzanji, Emtenan Basahl, Samah Zahran, Saddiq Habiballah, Emad Anam

Journal of King Abdulaziz University: Medical Sciences

Introduction: Autoimmune diseases, particularly in pediatric patients, present unique challenges that require awareness and support in school environments. Raising awareness among students and educators can enhance understanding, reduce stigma, and foster inclusive environments for individuals affected by these issues.Objective: This study aimed to assess the impact of an educational campaign in high schools in Saudi Arabia, focusing on increasing knowledge and fostering supportive attitudes toward children with autoimmune diseases.Methods: A total of 180 female high school students participated in a 30-minute lecture that highlighted general information about autoimmune diseases, with a specific focus on pediatric conditions. The presentation …

Menstrual Changes Following Covid-19 Vaccination Among Outpatient Reproductive-Age Visitors At King Abdulaziz University Hospital: A 2023 Cross-Sectional Study In Jeddah, Saudi Arabia, Abdulrahman T. Abudawood, Iman M. Wahby, Hala A. Bagabir, Najla M. Almudayni, Alya N. Alkhunaizi, Asma A. Alfarsi, Taghreed F. Alnahari

Journal of King Abdulaziz University: Medical Sciences

Background: The potential link between COVID-19 vaccination and menstrual disturbances has raised significant concern. Women who have experienced menstrual changes are apprehensive about receiving booster doses due to concerns about the duration of these side effects.

Objectives: To determine whether COVID-19 vaccines are associated with menstrual disturbances by assessing the vaccine’s impact on various aspects of the menstrual cycle, including cycle length, amount of bleeding, and pain.

Method: A cross-sectional analytic study was conducted using an online questionnaire among women of reproductive age (18-35 years) at King Abdulaziz University Hospital. Participants were recruited between December 2022 and January 2023 and …

The Association Of Apolipoprotein A1 And B With Glycated Hemoglobin Among Adults In Saudi Arabia, Hussam Daghistani, Abdulrazaq A. Bahaidrah, Osama A. Altookhi, Albaraa A. Marzuqi, Abdulaziz F. Binymin, Mohammed Algethami, Hadiah B. Al Mahdi, Aliaa A. Alamoudi, Yousef Almoghrabi, Motasim Jawi, Mehal A. Alqriqri, Maha A. Alqriqri, Zuhier A. Awan

Journal of King Abdulaziz University: Medical Sciences

Diabetes mellitus (DM) is one of the most prevalent chronic diseases worldwide, with a high burden in the Middle East, particularly in Saudi Arabia. Apolipoproteins, including apolipoprotein A1 (ApoA1) and apolipoprotein B (ApoB), play essential roles in lipid metabolism and cardiovascular disease, and have emerging associations with glycemic control. This retrospective cohort study investigated the relationship between ApoA1, ApoB, and glycated hemoglobin (HbA1c) levels in Saudi adults and examined the mediating role of body mass index (BMI) in these associations. HbA1c was measured by turbidimetric inhibition immunoassay (TINIA) on the Alinity c system, and ApoA1 and ApoB were assessed via …

Impact Of A Nutrition Education And Counseling Program On Diet, Lifestyle, And Cardiometabolic Health In Adults With Overweight And Obesity: A Non-Randomized Controlled Trial, Jeddah, Saudi Arabia, Eram Albajri, Elham Aljaaly, Abeer Aljahdali, Manal Naseeb, Mai Khatib, Nahlaa Khalifa, Nagat E. Eltoum, Aisha Y. Hussein

Journal of King Abdulaziz University: Medical Sciences

Background: Obesity is a global health issue attributed to changes in dietary habits and inadequate physical activity.Objective: This research aimed to assess the effectiveness of the nutrition counseling and education program on dietary habits, physical activity, and biomarkers among adults.Methods: This non-randomized controlled trial was conducted over 30 days in a primary care setting. Participants were grouped according to whether they chose to join the intervention program (n = 40) or maintain their regular diet (n = 20). The intervention group received personalized meal plans, recipes, and physical activity recommendations in line with the Saudi guidelines for obesity …

Variability Within Orthopedic And Plastic Surgery Hand Training In The Western Region Of Saudi Arabia, Bayan A. Ghalimah, Maan T. Almaghrabi, Maha S. Bamatraf, Ranad M. Khashab, Nada A. Alshaikh, Mohammed A. Alahmari, Abdulaziz A. Almutairi, Seham A. Alahmari, Rasha E. Alotaibi, Amre S. Hamdi

Journal of King Abdulaziz University: Medical Sciences

Background: Hand surgery is a challenging subspecialty shared by orthopedics and plastic surgery. Variations in training exposure and methods within residency programs, both globally and in Saudi Arabia, affect the clinical expertise of hand surgeons. This study aims to assess the variability in hand surgery training between orthopedic and plastic surgery residents in the western region of Saudi Arabia. Objectives: To evaluate differences in training exposure, satisfaction, and procedural experience in hand surgery training among orthopedic and plastic surgery residents. Design: A cross-sectional descriptive study was conducted using an anonymized, validated questionnaire. Setting: Residency training programs in the western region …

Decoding The Girl Code: Exploring The Unwritten Norms In Female Adolescent Friendships, Lindsay Angela F. Azura, Annica Mariz G. Ilagan, Krystine Jean O. Obia, Natalie Allyana P. Talplacido, Stella P. Go

Sinaya: A Philippine Journal for Senior High School Teachers and Students

The girl code refers to a set of unspoken social rules that shape how adolescent girls form, maintain, and navigate close female friendships. These unwritten norms influence how they provide support, handle emotional challenges, and navigate moral decisions. Despite its significant impact on female adolescent relationships, research on the girl code, especially within the Philippine context, remains limited. This qualitative exploratory study examines how the girl code operates among cisgender Filipino adolescent girls, focusing on its key aspects, underlying values, development, enforcement, and consequences. Five focus group discussions (FGDs) were conducted among close-knit female friendship groups of senior high school …

From Companionship To Grief: Lived Experiences Of Adolescent Dog Owners At De La Salle University, Dave Edrich Lao, Oel Bes, Zachariah Moscardon, Renzy Sabandal, Noel Bedia

Sinaya: A Philippine Journal for Senior High School Teachers and Students

Pets have long been part of people’s lives, but today, they are often viewed as family, especially by adolescents who rely on them for comfort, support, and companionship. For many, the bond with a pet runs deep, making their loss emotionally devastating. However, the emotional and psychological impact of this grief, particularly among adolescents, remains largely overlooked in academic research despite growing recognition of the human-animal bond. This phenomenological study explores the lived experiences of seven adolescent dog owners (ages 18–23) from De La Salle University Manila, focusing on understanding how they form attachments to pets, navigate caregiving responsibilities, and …

From Universities, Streets, To Modern Contemporary Society: Becoming, Being, And Living As "Babaeng Aktibista", Mariah Alyssa Lorico Ampat, Jameelah Mikaellah Ahliya C. Robles, Zedrick Sombria Roa, Jessica Louise Cruz Clauna, Leo Vicentino

Sinaya: A Philippine Journal for Senior High School Teachers and Students

Historically, women faced patterns of prejudice and gender-based oppression, resulting in the denial of their fundamental rights. Through years of ingrained subordination under authoritarian regimes, activism birthed various movements and organizations that sought liberation. As militant groups gained traction, withstanding large-scale issues, women’s struggles were seldom addressed within nationalist movements. Literature has documented the roots of activism, women’s organizations, and the socio-political conditions throughout Philippine history. However, there is a narrow understanding of women’s experiences and how these mold their individual identities in response to shared struggles. This study attempts to design a local framework elucidating the motivations and drivers …

The Correlation Between Clinical Manifestations And Mortality In Covid-19 Infection, Wail A. Tashkandi

Journal of King Abdulaziz University: Medical Sciences

Background: The Coronavirus disease 2019 (COVID-19) pandemic affected more than 700 million individuals worldwide and resulted in the death of over 7 million people. Still, data are scarce on whether specific clinical manifestations of COVID-19 infection correlate with increased mortality rates. Objective: To study the correlation between clinical manifestations and mortality in a cohort of Saudi patients with COVID-19 infection. Methods: This was a retrospective (both descriptive and analytical) study of medical records conducted at a tertiary healthcare center in Jeddah, Saudi Arabia, using a large dataset collected between April and December 2020. The clinical manifestations of patients were studied …

Knowledge And Attitude On Analgesic Use Among Female Students At King Abdulaziz University In Rabigh, Saudi Arabia, Abdulrahman Abudawood, Iman Wahby, Haneen Aseeri, Jamila Bukhari, Rawan Almutairi, Ghadi Alsharif, Futoon Almutairi, Tawfik M. Ghabrah, Jamil A. Samkari

Journal of King Abdulaziz University: Medical Sciences

Background: Self-medication, specifically with analgesics such as paracetamol and non-steroidal anti-inflammatory drugs (NSAIDs), is prevalent among university students. This behavior can potentially lead to health risks, including hepatotoxicity and gastrointestinal complications. This study aimed to evaluate knowledge, attitudes, and practices regarding analgesic use among female students at King Abdulaziz University (KAU) in Rabigh, Saudi Arabia.

Methods: A cross-sectional survey was conducted in 2022, involving 371 participants. The questionnaire collected socio-demographic information, knowledge about analgesic side effects, attitudes toward their use, and practices during examination periods.

Results: Most students (65.4%) showed a moderate understanding of analgesics, with medical and nursing students …

Potencies Of Topical Corticosteroids Available In Saudi Arabia, Amal A. Kokandi

Journal of King Abdulaziz University: Medical Sciences

Background: Topical corticosteroids (TCs) are used extensively for different skin disorders. They differ in potency, which is measured by different methods. This study aimed to examine the potencies of all TCs registered with the Saudi FDA. Methods: All Saudi FDA-registered TCs were matched to the US classification (7 categories), the Cochrane classification (4 categories), and the ATC 4-category classification (4 categories), if available.Results: A total of 116 products were identified, containing 16 known TCs. All products were classified to the best of our knowledge. There were a few uncertainties about the classification of some TCs.Conclusions: There is a …

Awareness And Misconceptions About Cardiovascular Disease Risk Factors And Related Behaviors Among Medical Students And Interns In Jeddah, Saudi Arabia, Waad S. Alhumaidi, Abdullah M. Alagha, Mariyam A. Aljadani, Faisal A. Almahyawi, Shahad S. Balubaid, Abdelrahman M. Attia, Shimaa M. Yousof

Journal of King Abdulaziz University: Medical Sciences

Background: Cardiovascular disease (CVD) remains the leading cause of death worldwide, making awareness of its risk factors and the adoption of healthy behaviors critical, especially among future healthcare professionals. This study aimed to assess awareness of CVD risk factors, misconceptions, and health-related behaviors, in addition to the relationship between academic year and CVD awareness among medical students and interns at King Abdulaziz University in Jeddah City.

Methods: A cross-sectional survey was conducted on 381 medical students and interns. Participants answered questions about CVD risk factors, health behaviors, and the sources of information. A linear regression analysis was performed to explore …

The Role Of Artificial Intelligence In Enhancing Infection Control In Hospitals: A Systematic Review, Khalid M. Alquthami, Ali M. Alahdal, Sadiq A. Serdar, Mazin M. Kheyami, Mohammed A. Alwadani, Razaz A. Barashi, Mohammad M. Alkhozaee, Basem A. Jawa

Journal of King Abdulaziz University: Medical Sciences

Hospital-acquired infections represent a critical global healthcare challenge, affecting 7–10% of hospitalized patients worldwide with 1.4 million annual deaths. This systematic review examines the transformative potential of artificial intelligence in infection control through predictive analytics, real-time surveillance, and antimicrobial stewardship. Analyzing 50 peer-reviewed studies, we demonstrate the effectiveness of AI tools in reducing infection rates by 35% and improving antibiotic utilization by 90%. We identify key implementation barriers, including data integration challenges, algorithmic bias, and ethical concerns. By synthesizing real-world case studies and proposing a three-phase implementation model, this review bridges the gap between technological capabilities and hospital infection control …

Strongyloides Stercoralis Infection In Humans, Shaymaa A. Abdalal

Journal of King Abdulaziz University: Medical Sciences

Strongyloides stercoralis (S. stercoralis), commonly known as the threadworm, is a soil-transmitted helminth that infects humans and causes strongyloidiasis. Despite being a significant public health concern, it remains one of the most neglected tropical diseases. The infection is prevalent worldwide, except in Antarctica, and often results in asymptomatic chronic gut infections that can persist for decades. However, in immunocompromised individuals, particularly those on long-term corticosteroid therapy, hyperinfection syndrome can occur, leading to mortality rates as high as 87%. Challenges in managing strongyloidiasis include its wide range of clinical presentations, the lack of highly sensitive diagnostic tests, and the potential for …

Cesium-137 Accumulation In Marine Fish And Associated Dose Exposure: A Global Review, N.A.A. Kamarulzaman, M.S.M. Sanusi, H.N. Idris

Journal of King Abdulaziz University: Marine Science

Establishing baseline data for natural radiation exposure and radioactivity levels is essential for radiological health and safety programs, particularly during the implementation and development of nuclear power initiatives. This review evaluates gamma radiation exposure and anthropogenic cesium-137 (¹³⁷Cs) levels in the world’s oceans and marine fish, analyzing their radioecological impact. Data from 30 peer-reviewed publications indicate that ¹³⁷Cs activity concentrations in seawater range from 0.07 Bq kg^–1 to 6.4 kBq kg^–1, with fish values between 0.01–3.50 kBq kg^–1. Regression models developed from these studies predict the correlation between ¹³⁷Cs levels in …

Lead Bioremoval By Halotolerant Bacteria Isolated From Mangrove Rhizosphere Soils Of Saudi Arabia, Reda H. Amasha, Rawan A. Alhazzani

Journal of King Abdulaziz University: Marine Science

Bioremediation of hypersaline environments contaminated with heavy metals is challenging due to high salinity levels, particularly when using conventional microorganisms. This study aimed to isolate and identify halotolerant and halophilic bacteria from various mangrove rhizosphere soil samples in Saudi Arabia and to evaluate their ability to remove heavy metals under high-salinity conditions. Out of 27 isolated strains, LD57 and LR40 demonstrated the highest resistance to lead ions. Both isolates grew well at 37 °C in the presence of 3 M NaCl and recorded growth at varying concentrations of lead nitrate, ranging from 100 to 4500 ppm. The lead’s minimum inhibitory …

A Systematic Literature Review Of Sustainable Ports Strategies And Initiatives In The Arabian Gulf Ports – Lessons For Saudi Arabia And Uae, Osayd Helal, Jane Haider, Jean-Paul Skeete, Sehwa Lim

Journal of King Abdulaziz University: Marine Science

This systematic review provides a comprehensive evaluation of sustainability strategies in Arabian Gulf ports, focusing on the interplay between technological advancements, green initiatives, and regulatory challenges. While Industry 4.0 technologies—such as automation, artificial intelligence, and electrification—have been integrated to enhance operational efficiency and reduce carbon emissions, the extent to which these innovations align with regional policy frameworks and geopolitical constraints remains insufficiently analysed.

To address this gap, this review synthesises insights from 25 peer-reviewed studies, employing a comparative framework to assess the adoption of sustainability practices in Arabian Gulf ports, focusing on their approaches toward sustainable port development. It is …

Assessment Of Microplastic Pollution In Economically Significant Fish Species From Libya’S Northeastern Mediterranean Coast, Mousa Kateesh, Tarek A. Temraz, Rafat Afifi Khattab

Journal of King Abdulaziz University: Marine Science

Microplastic pollution in marine ecosystems has become a pressing global environmental issue. However, there is limited information on the distribution of microplastics in Libyan territorial waters. This study investigates plastic waste pollution in five commercially important fish species from various marine habitats along the eastern Libyan coast (Ain El-Ghazala, Sousa, Ras Hilal, Alhaniyah, and Alhamama). A total of 75 fish specimens were collected, comprising 15 individuals from each species. The findings revealed that 38% of the sampled fish had ingested plastic debris, with microplastics being the predominant form. The Sousa region exhibited the highest contamination rate (54.46%), while Alhaniyah had …

Urbanization Impacts On Microplastic In Benthic Organisms And Sediment Of Small Islands, Andrio Wibowo, Suyud Warno Utomo

Journal of King Abdulaziz University: Marine Science

Indonesia is an archipelago country comprising thousands of islands, including small islands. Currently, small islands have experienced urbanization in the forms of land use developments and conversions from pristine ecosystems to settlements and tourism facilities, leading to the contamination of microplastics. Despite growing numbers of research on microplastic in water, there is still limited information about microplastic in the sediment and benthic, in particular in developed small island environments. This study compared the types of land use on the island from reserved areas, tourism, and settlement. The microplastics were characterized based on the shapes. The results confirm that land use …

New Fish-Based Multimetric Index For Appraising Shatt Al-Arab Ecological Quality, Laith Faisal Lazem

Journal of King Abdulaziz University: Marine Science

In recent years, there has been a growing need to develop biometric indicators that accurately reflect an area’s environmental quality and biological integrity. Various indicators have been developed and analyzed, focusing on functional, ecological, and organism-based indicators, particularly fish-related ones. The purpose of the current paper is to create the first fish-based multimetric index designed to evaluate the quality of water in the Shatt Al-Arab River (SAAR), known as Shatt Al-Arab Fish Index (SFI), which can also be applied to the Qarmat Ali River and other water bodies similar to the SAAR ecology. Data was collected from three stations along …

Trajectory Prediction Of Oil Spills In Sharm Obhur, Red Sea Using Hydrodynamic Modeling, Mohammed A. Bahattab, Gar Al-Nabi Ibrahim, V.R. Shamji

Journal of King Abdulaziz University: Marine Science

This research investigates the movement and impact of oil spills in Sharm Obhur, a coastal creek near Jeddah, in the Red Sea. Utilizing a high-resolution multi-beam echo sounder and the Delft3D-FLOW hydrodynamic simulation model, the study traces the oil spill trajectories, considering key factors such as meteorological conditions, spill volume, and oil properties. The model predicts the movement of the oil spill both within Sharm Obhur and its progression towards the Red Sea. Results from the model are intended to assist in the development of effective spill response strategies, improving navigation safety, protecting coral reefs, and preserving the local ecosystem. …

Preprocessing Improves The Edna Quality For 16s Rrna Metabarcoding Of Sediment Samples Of Solar Salterns, Jayanath Gopi, Bhavya Kachiprath, Solly Solomon, Sowmya P. Mohandas, Sajan C. Raju, Rosamma Philip

Journal of King Abdulaziz University: Marine Science

The present study examines the efficacy of pre- processing of sediment samples prior to DNA isolation to obtain high-quality eDNA for metagenomic analyses from crystallizer saltern pond in Goa during the salt harvesting phase. The quality of the extracted eDNA were confirmed through spectrophotometric analysis and PCR amplification of the 16S rRNA gene. The study showed that method 2 and 3 incorporating preprocessing of the sediment samples yielded better quality eDNA compared to the direct isolation protocol (method1). By adopting the best method i.e., preprocessing by sediment suspension- filtration, followed by DNA isolation, the eDNA was procured and subjected to …

Morphometric, Physiological, And Environmental Health Assessments Of Enhalus Acoroides From The Southeast Coast Of Bali, Indonesia, Made Pharmawati, Luh Putu Wrasiati, Ni Made Gari

Journal of King Abdulaziz University: Marine Science

Seagrasses function as primary producers and as spawning grounds and shelter for marine biota. Regardless of their relevance, seagrasses are under threat due to anthropogenic impacts and an increasing frequency of extreme climate events. Enhalus acoroides is a species of seagrass that is spread in the east and southeast waters of Bali, from Sanur Beach to Nusa Dua Beach. This study aims to analyze the differences in morphometry and the chlorophyll, proline, and malondialdehyde (MDA) content, a marker for oxidative stress in E. acoroides leaves at Pengastian Beach, Semawang Beach, and Samuh Beach, Bali. Samples were collected using line transect …