Digital Commons Network^™

Unraveling The Elasmobranch: A Look At Shark Oral Microbiomes In South Florida, Adam Sadik, Adhvika Saravanan, Amanda Finnefrock, Melody Kim

Undergraduate Student Symposium

The intricate relationship between host organisms and their associated microbial communities, known as the microbiome, has garnered increasing attention across various biological disciplines. Sharks, as apex predators in marine ecosystems, are important to study and understand. Previous studies have shown shark gills and oral cavities are abundant in various types of commensal and pathogenic bacteria, many of which are resistant to antibiotics. Previous research on Florida blacktip sharks suggest 43% of all bacteria samples from the sharks were resistant to at least one antibiotic. Using microbial culturing techniques and Next Generation 16S ribosomal RNA (rRNA) sequencing, our objective is to …

Exploring The Psychological Impact Of In-Vitro Fertilization, Japji G. Madan

Undergraduate Student Symposium

In Vitro Fertilization (IVF) affects both men and women differently, impacting their physical and emotional health throughout the process. Understanding these dynamics is essential to offer support to couples navigating the complex journey of assisted reproduction. This paper delves into the multifaceted stages of IVF, addressing the psychological and emotional challenges faced by individuals undergoing this invasive treatment. With the increasing prevalence of IVF as a common fertility treatment, this review emphasizes the importance of considering both the short-term and long-term psychological implications for couples.

Coconut Oil As A Renewable Source For Carbon Dot Nanoparticles: Synthesis And Characterization, Amaan Khan, Aryan Rathi, Srika Saai Talanki, Pierce Canavan, Tasfia Tazri Howlader, Maria Guillén-Méndez

Undergraduate Student Symposium

Carbon dots (C-dots) are zero-dimensional, carbon-based fluorescent nanoparticles that have gained significant attention due to their exceptional optical, chemical, and physical properties, along with their biocompatibility, cost-effectiveness, and facile synthesis. These characteristics make C-dots versatile for applications in biomedical fields, food packaging, anti-counterfeiting, and semiconductors. Despite the exploration of natural products for C-dot synthesis, using oils to synthesize C-dots remains underexplored. In this study, we synthesized C-dots using coconut oil, a lauric acid-rich renewable resource, through a bottom-up approach. A mixture of coconut oil, citric acid, and ethylenediamine was treated at 180°C for 12 hours, followed by purification via centrifugation …

Long Noncoding Rna Malat1 In Mitochondrial Modulation Induced By Ketosis In Human Cardiomyocytes, Meghana Divi, Eera Mehrotra, Colin Pulickathadam, Sathvi Gandra

Undergraduate Student Symposium

Heart failure, a major cause of mortality, is increasingly viewed as a metabolic disorder involving mitochondrial dysfunction. Ketone body treatments have shown promise in enhancing mitochondrial function and improving heart failure outcomes. Long noncoding RNAs (lncRNAs), which are RNA molecules longer than 200 nucleotides that do not code for proteins, play critical roles in regulating gene expression and mitochondrial activity. Despite the known modulation of lncRNAs during heart failure, their specific functions under ketotic conditions remain poorly understood. Based on our preliminary findings, this study aims to explore the impact of ketosis on long noncoding RNAs (lncRNAs), particularly MALAT1, and …

Protecting Citrus Through Patents: Legal And Economic Implications Of Uf/Ifas Biotechnological Advances, Laeila Scott

Undergraduate Student Symposium

Citrus greening, or Huanglongbing (HLB), threatens the survival of Florida’s citrus industry, driving innovation in disease-resistant crops through biotechnology. This research examines how intellectual property (IP) law shapes these advancements, focusing on the direct industry impact of the University of Florida’s Institute of Food and Agricultural Sciences (UF/IFAS). Through analyzing patent strategies, licensing models, and governance structures, this study explores the balance between proprietary protections and public access. Additionally, it considers broader ethical and economic implications, including the challenges of patent enforcement and the impact on citrus growers. This research situates UF/IFAS’s biotechnological innovations within the larger legal and economic …

Modeling Isocitrate Dehydrogenase 2 Mutations In Acute Myeloid Leukemia: Exploring How The Drug Ag-221 Inhibits 2-Hydroxyglutarate Production And Restores Blood Cell Differentiation, Bhumika Balani, Jay Matta, Brianna Bourke, Nitya Punjal, Melissa Dore Ph.D.

Undergraduate Student Symposium

Mutations in the Isocitrate Dehydrogenase 2 (IDH2) enzyme have been linked to acute myeloid leukemia (AML). IDH2, involved in the citric acid cycle, normally converts isocitrate to α-ketoglutarate (α-KG), using NADP+ to produce NADPH. Mutations like R140Q and R172K lead IDH2 to convert αKG into the oncometabolite 2-hydroxyglutarate (2HG), hindering blood cell differentiation and resulting in AML.

The drug AG-221 targets mutant IDH2 at an allosteric site, stabilizing the enzyme in an inactive form and preventing 2HG formation, thus alleviating AML symptoms. Most patients, however, require additional chemotherapy and often complete bone marrow transplants. A 3-D printed model based on …

Nutritional Challenges In Children With Down Syndrome And The Use Of Early Interventions, Avisha Thota

Undergraduate Student Symposium

Children with Down syndrome (DS) face distinct nutritional challenges that significantly impact their growth, health, and overall quality of life. These challenges include a high prevalence of obesity, gastrointestinal issues, increased risk of oral motor difficulties, and eating disorders such as pica and avoidant/restrictive food intake disorder (ARFID). Contributing conditions include unique low muscle tone, reduced physical activity, and metabolic differences, further compounded by environmental influences such as parental feeding practices and limited access to specialized care. The purpose of this experiment is to explore the nutritional challenges and eating disorders faced by children with DS and examine how early …

Determining The Optimal Multiplicity Of Infection (Moi) And Lysis Time Of Gordonia Rubripertincta Bacteriophage, Bianca A. Gonzalez, Jose C. Padron, Dani Sheil

Undergraduate Student Symposium

Antibiotic resistance represents a significant global health crisis, especially within hospitals, creating a necessity for the search for alternative therapeutic agents to combat drug-resistant pathogens. One alternative treatment is phages specifically targeting G. rubripertincta, a gram-positive, aerobic actinomycete bacterium which has shown intrinsic resistance to some antibiotics. Furthermore, G. rubripertincta can cause endocarditis and CNS infections in immunocompromised individuals. Several phages such as Alyssamiracle, Fribs8, Genamy16, and NovaSharks, have been discovered that successfully target G. rubripertincta. The goal of this research is to determine the optimal lysis time and multiplicity of infection (MOI) of these phages. The phages …

Sexual Dimorphism In Physiological, Metabolic, And Hypothalamic Alteration In The Tg-Swdi Mouse Model Of Alzheimer's Disease, Yingying Zhu, Ariana Hernandez, Victoria Pulido-Correa, Chana Vogel, Natalia Noto, Julianna Bonetti

Undergraduate Student Symposium

Alzheimer’s disease (AD) is a neurodegenerative disorder affecting 55 million people worldwide. Cognitive disruptions, linked to pathology in the hippocampus and cortical areas, are frequently observed in individuals with late manifestation of AD; however, non-cognitive dysfunctions, which contribute to poor quality of life and survival, are often overlooked. The hypothalamus plays a role in regulating homeostatic functions such as hunger, thirst, sleep, and blood pressure. The coordinated activity of hypothalamic nuclei regulates energy balance and hormonal functions, which are crucial for maintaining metabolic homeostasis and neuroendocrine processes. Dysregulation of these processes has been shown to directly correlate with amyloid-β and …

Investigating The Therapeutic Potential Of Mdm2 And Dnmts Inhibition In Non-Small Cell Lung Cancer, Nathan Tinu, Samhitha Vangala

Undergraduate Student Symposium

Lung cancer is one of the leading causes of cancer-related deaths, underscoring the need for more effective therapeutic strategies. This study explores the impact of MDM2 and DNMTs inhibition on key regulatory pathways in the HCC827 lung cancer cells, specifically focusing on epigenetic modification and cell cycle regulation. We investigated the effects of RG-7388 (MDM2 inhibitor) and CM-272 (G9a/DNMTs inhibitor) on MRNAs and miRNAs gene expressions that were suspected to be modulating the above-mentioned processes. Our qRT-PCR results showed significant downregulation of epigenetic modifiers (DNMT1, DNMT3A, DNMT3b) and cell cycle arrest genes (CDC25A, CDC25C) in both RG-7388 and CM-272 treated …

Analysis Of Community Impact And Sustainability: A Comparative Of Humana Inc. And Toyota Motor North America, Dev J. Patel

Undergraduate Student Symposium

Climate change affects all aspects of human life. With the depletion of the Ozone layer, increasing frequency of natural disasters, health impact, and more, expectations must be set for large corporations to follow the principle of sustainability. This concept focuses on how businesses can prioritize environmental health, social justice, and economic development of nearby communities they serve. The most common practice for reporting on sustainable issues is through annual iterations of environmental, sustainable, and governance (ESG) reports. This qualitative study examines the sustainability efforts of two major corporations: Humana, Inc. and Toyota Motor North America (TMNA). Humana, Inc. is the …

Decrypting Unknown Viruses: Characterizing Two Unique Bacteriophages, Razorc And Astralis, Through Genome Sequence Annotation, Lidhu Arakkal, Chris Cherian, Vivian Luong, Yuktha Milukuri, Hrishi Paliath-Pathiyal, Hansi Parmar, Shruthi Senthil, Jerqual Smith, Ashley Thompson, Chanakya Prasad Valmiki, Despina Varghese, George Vasilakis, Jyothi Vivekananda, Kayle Mederos, Swara Chindhade, Dev J. Patel, Earlissa Tucker, Rishubh Madaboosi, Mehtab Singh

Undergraduate Student Symposium

In 1915, Frederick William Twort and Felix d’Herelle discovered bacteriophages (phage), viruses that kill bacteria. As this was prior to the discovery of antibiotics, their work led to early development of phage therapy, treatment that involves the use of phage to selectively kill bacterial. Today, phage therapy provides an alternative to antibiotics especially by targeting specific pathogens that are normally resistant to multiple treatments. Even with its ability to alleviate the antibiotic-resistant problem, the vast majority of phage remains uncharacterized. Therefore, it is important to expand bacteriophage discovery-based and genome characterization research. Through the SEA-PHAGES (Science Education Alliance – Phage …

Solvent Effect On The Stabilization Of The Monoprotonated Species Of Tetraphenylporphyrin, Pablo Perez, Dani Sheil

Undergraduate Student Symposium

The protonation of Tetraphenylporphyrin (TPP) demonstrates unusual behavior where protonation causes only the formation of a diprotonated species. This behavior is due to a change in conformation leading to an unstable saddle deformation in which the monoprotonated species is highly unfavorable. The stabilization of the unfavored conformation in the monoprotonated intermediate species may be achieved by understanding the effects of different solvents on the protonation of TPP. This study was done to explore the protonation of TPP using Toluene Chloroform, and Dichloromethane using trifluoroacetic acid and chloroacetic acid to analyze the effects of the different solvents. Using a UV spectrophotometer, …

Synergistic Effects Of Quercetin And Lutein In Tagetes Erecta Against Antibiotic-Resistant Microorganisms, Ninoshka I. Garcia Torres

Undergraduate Student Symposium

The increasing global problem of antibiotic resistance has driven the need to look for innovative classes of antimicrobial agents. Phytochemicals isolated from Tagetes erecta, such as Quercetin, a flavonoid, and lutein, a carotenoid, have antimicrobial properties against bacteria through various mechanisms, such as disruption of bacterial membrane integrity, production of reactive oxygen species, and biofilm inhibition. Quercetin and lutein also improve the effectiveness of standard antibiotics, indicating their usability as adjuvants against resistant microbes such as Staphylococcus aureus and Pseudomonas aeruginosa. Despite their potential, several challenges need to be addressed, including the fluctuating yield production of phytochemicals, the …

The Effects Of Early Mobilization On Rotator Cuff Tears: A Meta Analysis, Colton Batz

Undergraduate Student Symposium

The purpose of this research is to assess post-surgical early mobilization of the rotator cuff compared to conservative treatment and the impact on return-to-participation. BACKGROUND: Rotator cuff tears are common upper-body injuries in sports such as baseball, volleyball, and handball, requiring surgical intervention in 21% of reported rotator cuff tears among volleyball players, and often dependent on tear size and severity of tear among athletes. Although, some post-surgical rehabilitation programs may provide quicker return-to-participation times compared to others, further research may provide insight into the impact of early mobilization. (P:) Rotator cuff patients looking for effective and prompt …

The Role Of Future Time Perspective And Social Support In Shaping Psychological Well-Being During Emerging Adulthood, Amala Vadukumcherry, Japji G. Madan, Kaleigh Cummings, Riya Vadaliya

Undergraduate Student Symposium

The present study explores the associations between future time perspective and perceptions of social support and their influence on psychological well-being in emerging adulthood. The sample consisted of 334 emerging-adults between the ages of 18-25(average-age18.76years, 267women). Subjects completed self-report measures of future time perspective, perceptions of social support, and psychological well-being.

Regression-results indicate that social-support and future time perspective together predict psychological well-being. Follow up tests using the procedures recommended by Aiken and West (1991) indicate that social support moderated the links between future time perspective and psychological well-being.

Specifically, emerging-adults with low-medium family support who see their future as …

The Universal Mass Function And Its Applicability To Organic Molecules, Gabrielle Andrews, Taylor Butler

Undergraduate Student Symposium

The ability to predict the yield when synthesizing an organic molecule is a challenging issue in organic chemistry and a major obstacle when planning multi-step organic synthesis. The Universal Mass Function (UMF) states that cosmic objects are in direct relation to each other and suggests that massive objects are much rarer than objects with lower masses. As a result, the following research aims to examine if the UMF theory is applicable to the field of organic chemistry, specifically to the yield prediction. The primary reaction conducted was completed in an aprotic environment. This reduction was performed on several different ketone …

Adoptive Transfer Of B10 Cells Promotes Resolution Of Periodontal Inflammation, Jayant Ari

Undergraduate Student Symposium

Regulatory B cells (Bregs) play a crucial role in reducing the inflammation of periodontitis. It has been shown that specialized pro-resolving mediator (SPM) synthesis and production mediate the resolution of periodontitis inflammation and alleviate bone resorption. Bregs has been reported alleviate the inflammation in periodontitis, but little is known about their function in SPM production. The present study aims to investigate whether IL-10 expressing regulatory B cells (B10) can inhibit periodontitis progression by promoting the production of SPMs. Experimental periodontitis models were established in mice by ligation combined with periodontal pathogen P. gingivalis bacteria inoculation for 14 days, and B10 …

The Role Of Attachment And Self-Esteem In Identity Development Among Emerging Adults, Akhila Nalluri, Preetham Gopu, Charvi Thumati

Undergraduate Student Symposium

Emerging adulthood, a transitive period between 18 and 25 years, is widely considered a period marked by change, experimentation, exploration, and development. Attachment theory postulates that relationships between children and their caregivers are crucial for the organization/representation of the self. Extant research indicates that secure parent-child-relationships and identity-development co-exist during emerging-adulthood. Self-esteem has also been associated with secure identity-formation. While there is considerable research on the associations between parent-child relationships and identity formation, as well as self-esteem and identity-formation, relatively little is known about the combined effects of parent-child relationships and self-esteem on identity-development during emerging-adulthood.

The present study explores …

A Man In The Woods: Individual Differences In Women's Distress Response To Unknown Men, Tamara Bogdanova, Brianna Muñoz, Ned Vaitkevicius

Undergraduate Student Symposium

The aim of this study was to investigate the relationship between distress and perceived danger in modern humans since previous research has shown that threats have various effects on an individual’s thoughts and behaviors. We hypothesized that women would report greater changes in distress after reading a story in which they are confronted with an unknown man compared to after reading a story in which they interact with a friend. Additionally, we hypothesized that women’s change in distress would be moderated by individual difference variables such as mate value. Using an experimental vignette methodology, data provide preliminary support for both …

Eat-26 As A Stable Measure Of American College Athletes: Diet, Food, Preoccupancy, And Calorie Counting As Predictors Of Health, Simrin Raju

Undergraduate Student Symposium

This study examines the structure and reliability of the Eating Attitudes Test (EAT-26) in a sample of 144 college athletes. The EAT-26 is commonly used to measure eating attitudes but is not intended to diagnose eating disorders. The study aims to confirm if the test's factor structure, which has been stable since its development in 1979, remains consistent in this new group. The results show that the EAT-26 continues to reflect three main factors—Dieting, Preoccupation with Food (Others Notice) and Calorie Counting (Oral Control). These factors accounted for the majority of the variation in responses, with "Dieting" being the most …

Effect Of Dopamine On Nlrp3 Inflammasome Activity And Thiol Metabolites In Human Thp-1 Macrophages, Grace K. Wong, Emily H. Guirguis

Undergraduate Student Symposium

Neuroinflammation plays an important role in aging and neurological disorders across the lifespan and the NLRP3 inflammasome has been closely linked to the progression of neurodegenerative diseases and is a potential target for therapy. The neurotransmitter dopamine regulates production of inflammatory cytokines in an age-dependent manner and in neurons dopamine is stored in a complex with ATP and both are released during neuronal activity. ATP promotes NLRP3 inflammasome activity via the P2X7 receptor. However, the combined influence of dopamine and ATP on inflammasome activity has not been previously investigated. The goal of this study was to examine lipopolysaccharide (LPS)-induced NLRP3 …

Effects Of Reproductive Experience On Cognitive-Behavioral Outcomes In Mouse Models Of Alzheimer's Disease And Related Dementias, Chana Vogel, Esperanza Barrios, Ariana Hernandez, Natalia Noto

Undergraduate Student Symposium

Dementias, including Alzheimer’s disease and vascular dementia, are devastating conditions lacking safe and effective treatments. Despite the striking sex/gender difference in the prevalence of dementia, few studies have investigated sex-specific factors that may influence the greater risk and faster progression of disease in females. These factors include but are not limited to prior pregnancy and motherhood. Women with children have slower rates of cognitive decline and lower risk for developing mild cognitive impairment or dementia than women with no children. However, other studies have reported mixed results, suggesting that the influence of reproductive/maternal experience may depend on an individual’s number …

Evaluating Diagnostic And Treatment Timelines For Alk-Positive Nsclc Patients: Results From A Global Registry For Consideration In The Journal Of Clinical Lung Cancer, Swara Chindhade, Aula Alqased

Undergraduate Student Symposium

ALK-positive non-small cell lung cancer (NSCLC) is an aggressive subtype affecting younger, nonsmoking individuals. Tyrosine kinase inhibitors (TKIs) are essential treatments; however, global data on comorbidities, diagnosis, treatment, and intervals from initial medical visits to TKI treatment remain limited. This study examines symptoms, comorbidities, and treatment timelines to identify factors contributing to delays in TKI treatment. We conducted a longitudinal observational study using a global registry of ALK-positive NSCLC patients via online surveys from September 2022 to November 2024. Participants were recruited through support groups, patient newsletters, and oncologist referrals. Descriptive statistics and multivariable regression analyses evaluated the impact of …

Examining The Impact Of Volleyball-Specific Movements On Landing Using Vert Wearable Technology, Camille Fonseca

Undergraduate Student Symposium

The sport of volleyball involves constant vertical and lateral motion, as it requires players to attack, block, or serve a ball. The frequency of jumps and landings creates stress on the body, predisposing female athletes to lower extremity injuries. Additionally, volleyball-specific movements such as the slide, swing block, and 7-ball, may cause injury. The purpose of this study is to assess jumps and landings from volleyball-specific movements in female collegiate volleyball athletes using VERT wearable technology. National Collegiate Athletic Association Division II Volleyball athletes wore the VERT device collecting data on performance and workload during team practices and games including …

Exploring Dual-Language Testing And Verbal Ability Performance In Non-Native English Speakers, Jaylyn Marte, Andrea Fajardo Aguilar

Undergraduate Student Symposium

Research suggests that Spanish/English bilinguals do not perform as well as English-speaking monolinguals on tests of verbal ability. Even when born, raised, and educated in the US, Spanish/English bilinguals do not perform as well as monolinguals. The current study was designed to investigate whether: (1) Spanish/English bilingual college students improve their performance on verbal ability tests when given the opportunity to respond in either of their two languages relative to responding only in English; and (2) do they perform similar to monolinguals under dual language testing. A previous study from our laboratory used the Bilingual Verbal Ability Test (BVAT) which …

Exploring Oral Health Challenges In Pateints With Als: Practices, Barriers, And Disparities, Eden Pressley

Undergraduate Student Symposium

Proper oral health is often neglected in vulnerable populations such as persons living with ALS (plwALS) due to the myriad of physical demands and limitations of the disease.¹ Poor oral hygiene increases the risk of aspiration pneumonia, with studies demonstrating oral bacteria in the pulmonary tract of patients with pneumonia, and contributes to oral discomfort, reduced quality of life and increased caregiver burden.^4-6 Although oral hygiene represents a modifiable risk factor for pulmonary sequalae, it is not routinely addressed in the current standard of care for ALS and our knowledge of oral health in plwALS is limited. Therefore, …

Feedback Literacy In Higher Education: A Mixed Methods Examination Into Student Perceptions Of Feedback Literacy & Feedback Processes Using The Deft Toolkit As A Training Intervention, Tiya Goins

Undergraduate Student Symposium

Feedback literacy has emerged as a critical skill in higher education, enabling students to actively engage with, interpret, and apply feedback to improve their learning outcomes. Despite its significance, there is limited research examining how students perceive their own feedback literacy and how they engage with feedback dependent mechanisms, such as Student Evaluation of Teaching (SETs). This study aims to investigate student perceptions of feedback literacy and feedback processes using the Developing Engagement with Feedback Toolkit (DEFT) as a training intervention. A mixed-methods approach was employed, incorporating pre- and post-intervention surveys, a scenario-based assessment in which students evaluate a mock …

Growth And Survival Of Sexually Propagated Land-Reared Brain Corals During An Intermediate Offshore Nursery Phase, Emma Horton

Undergraduate Student Symposium

Florida’s Coral Reef has degraded from significant stony coral loss due to global and local stressors, including heat stress, disease, and coastal development, leading to reduced natural coral recruitment. Assisted sexual propagation in land-based nurseries can increase the number of genetically unique corals available for restoration efforts. A grow-out phase, in which land-reared corals are placed into an offshore nursery before outplanting on the reef, is often employed to allow corals time to grow and acclimate to the ocean environment. However, research on the performance of recruits within offshore nursery settings remains limited. Here we examine the growth and survival …

Identifying Key Characteristics In Aspiring Healthcare Professionals: A Strengths And Preference Profile Analysis On Emotional Intelligence, Abbygail Salcido, Emily Young

Undergraduate Student Symposium

Topic: As undergraduate students in a pre-health path or an advisor for these students, understanding one's priorities, strengths, and motivations is crucial for self-awareness and career clarity. This understanding is pivotal in guiding students toward a fulfilling and appropriate career choice. Purpose: Our study aims to identify the characteristics of students with the potential to excel as Optometrists - a profession known for its emphasis on patient care, attention to detail, and creative intuition. In our current investigation, we assessed 125 students aspiring to become Optometrists. Each participant completed a strengths inventory and a positive psychology-based personal preference test. According …