Digital Commons Network^™

Exploring The Role Of Host Immune Responses And Bacterial Sensory Pathways In The Development Of Treponema Denticola-Mediated Periodontal Disease, Annie N. Hinson

Theses and Dissertations

Periodontitis is a chronic inflammatory disease driven by dysbiotic microbial communities and maladaptive host responses. Treponema denticola, a spirochete pathobiont, is strongly associated with disease severity. However, the molecular mechanisms underlying its interaction with host tissues and regulation of virulence remain incompletely understood. This work examines both the epithelial immune responses and bacterial transduction systems that shape T. denticola pathogenesis. Transcriptomic analysis of gingival keratinocytes revealed IL-36γ as the most highly induced cytokine following infection with T. denticola. Functional studies demonstrated that IL-36γ induction occurs in a dose- and time-dependent manner through TLR2/6 signaling, requiring the NF-κB, PI3K/Akt, and …

Functional Analysis Of Ligand Binding Domain 2 Of Outer Surface Protein C Of Borreliella Burgdorferi, Gavin Z. Chambers

Theses and Dissertations

Lyme disease, caused by the spirochete Borreliella burgdorferi, is the most common vector-borne infection in North America. A critical determinant of early mammalian infection is outer surface protein C (OspC), a highly variable lipoprotein that is indispensable for spirochete survival but whose precise function remains unresolved. OspC has been proposed to mediate diverse roles, including complement evasion, adhesion, tissue dissemination, and antiphagocytic activity, supporting the view that it is a multifunctional virulence factor. Structural studies have identified two putative ligand-binding domains (LBD1 and LBD2), with LBD2 forming a solvent-exposed crown at the membrane-distal end of the OspC dimer.

To …

Impact Of Costume And Rape Myths On Perceptions Of The Need For Consent In Interactions With Cosplayers, Kiana Monique Perez-Jimenez

College of Graduate Studies: Theses & Dissertations

Research on fandom communities is relatively understudied. Specifically, perceptions surrounding consent, rape myth acceptance, and other factors such as gatekeeping attitudes and experiences with discrimination are understudied and undertheorized (Hale, 2014). Historically, sexual harassment has been a pervasive issue in spaces like video gaming communities, especially toward women (Barak, 2005). It is important to further investigate these behaviors in different areas, such as conventions (i.e., cons), because, like in video gaming communities, sexual harassment has been an ongoing issue in con spaces (Zarin, 2017). The current study addressed how cosplayers' dress impacts key aspects of sexual harassment, including perceptions of …

South Asian American Experiences Of Microaggressions And Wellbeing: The Moderating Role Of Social Support, Archita Birla

College of Graduate Studies: Theses & Dissertations

Although the South Asian American community is rapidly expanding in the United States (South Asian Americans Leading Together [SAALT], 2015), there remains a dearth of psychological research regarding the impacts on mental health and wellbeing affecting this diverse community. Racial microaggressions, or verbal or behavioral actions indicating hostile or negative attitudes toward marginalized individuals (Sue et al., 2007), negatively impact People of Color (POC) mental health and wellbeing (Forrest-Bank & Jenson, 2015a; Nadal, Wong, et al., 2015). Some research exists on South Asian American communities and microaggressions (Houshmand et al., 2014; Poolokasingham et al., 2014) but there is limited research …

Use Of Scat Dna To Estimate Coyote Abundance On A Newly Colonized Barrier Island, Sonia M. Hernandez, Michael J. Yabsley, Sarah C. Webster, Elizabeth M. Kierepka, Rochelle R. Beasley, Carly L. Landa, Heather W. Barron, Kelly Sloan, Holly Milbrandt, James C. Beasley

Human–Wildlife Interactions

Coyotes (Canis latrans) have expanded in mainland eastern North America and are known to have successfully colonized numerous islands. However, there are scarce data on the densities of coyotes in these ecosystems, in which coyotes can have substantive negative impacts. Coyotes first colonized Sanibel Island, a barrier island off the western coast of Florida, USA, in 2011, and their presence has been linked to declines in several sensitive species. However, the population size of coyotes has only been estimated from anecdotal reports and camera traps. Our goal was to estimate the size of the coyote population on Sanibel …

Bird Strikes During Climb And Approach: A Need For Innovative Management Strategies, Richard A. Dolbeer, Michael J. Begier

Human–Wildlife Interactions

Wildlife Hazard Management Plans at civil airports in the United States cannot directly address threats posed by birds moving through aircraft climb and approach paths unrelated to nearby bird-attractant habitats such as landfills. As an iconic example, an Airbus 320 departing LaGuardia Airport (New York, USA) on January 15, 2009, struck a flock of migratory Canada geese (Branta canadensis) 8 km out at 884 m above ground level (AGL) that resulted in a water landing now known as the “Miracle on the Hudson.” We documented 1,841 and 458 strikes from 2009 to 2023 involving large (≥1.8 kg) and …

Refine Fuels Treatments In The Wildland–Urban Interface With Wildlife In Mind, Miles Becker, Catrin M. Edgeley, David Stoner

Human–Wildlife Interactions

Conserving urban tree and shrub cover provides many benefits for wildlife and people, yet often becomes a lower priority than fuels treatments to mitigate fire risk in the wildland–urban interface (WUI). We support the principle that considering multiple objectives on privately owned property is valuable and can be achieved. Data sharing and collaboration between wildfire experts and wildlife biologists could improve the quality and extent of managing both animals and fire. Furthermore, the adoption of statewide policy and municipal regulations for preventing wildfires in the WUI provides an opportunity to incorporate ecological functions in the same directives for a balanced …

Using Advanced Machine Learning To Evaluate The Spatial And Temporal Patterns Of Human–Bear Interactions In Maryland, Usa, Katherine A. Kurth, Jonathan K. Trudeau

Human–Wildlife Interactions

American black bears (Ursus americanus) are highly adaptable to human-dominated areas, which can lead to interactions with humans. Managing these interactions is a priority for many wildlife agencies in North America. Climate-induced shortages in natural food can exacerbate human–bear interactions, as bears are driven to seek alternative food sources in human-dominated areas. However, few studies have investigated the impact of climate or weather on the frequency and severity of human–bear interactions. In Maryland, USA, no research has examined the anthropogenic or environmental factors contributing to human–bear interactions, even though reported interactions have consistently increased since the 1990s. Our …

Student Centers: The Intersection Of Campus Ecology And Sense Of Community, Caitlyn A. Wine

College of Graduate Studies: Theses & Dissertations

Student centers are more than physical structures as they function as dynamic environments that serve as the hearthstone of campus, providing spaces for students to gather, connect, and engage. However, their role in fostering students’ sense of community remains underexplored. Grounded in campus ecology theory, this study explored whether time spent in the student center and specific forms of interaction predicted students’ sense of community. A quantitative survey design was employed, the Student Center and Sense of Community Intersection Survey (SCSCIS), to assess student center usage and perceived sense of community. Results indicated that time spent in the student center …

Exploring The Impact: Eating Disorder Treatment And Mortality In The United States, Brittany Tolbert

College of Graduate Studies: Theses & Dissertations

This study explores adolescent eating disorder trends in the United States between 1999-2013, identifies national eating disorder mortality trends, and analyzes data for in-patient adolescent hospital admissions. Additionally, this study uses public-facing data for residential eating disorder treatment centers to explore issues related to insurance coverage, gender inclusivity, and evidence-informed treatment practices. The findings will highlight gaps in data collection and policy, support advocacy, and contribute to the research into eating disorder prevalence, prevention, treatment outcomes, and economic impact, leading to greater visibility to promote change.

Application Of Machine Learning And Large Language Models In Healthcare For Data Prediction And Summarization, Chiazam Chisom Izuchukwu

College of Graduate Studies: Theses & Dissertations

This study aims to examine the use of machine learning (ML) and large language models (LLMs) in healthcare to enhance disease prediction, clinical decision-making, and information management. Five supervised ML models—Logistic Regression (LR), Support Vector Machine (SVM), Random Forest (RF), Decision Trees (DT), and Naïve Bayes (NB)—on three different computing platforms—Google Colab, Databricks, and Snowflake—were employed for disease classification. Data preprocessing included treating missing values, encoding categorical variables utilizing one-hot-encoding, feature scaling when needed, and tackling class imbalance with Synthetic Minority Over-sampling Technique (SMOTE) before an 80-20 train-test separation. Models were created with Scikit-learn (Google Collab), Spark MLlib (Databricks), and …

Literary Homeplace As A Site Of Knowing And Healing, Shamonique Hassell

College of Graduate Studies: Theses & Dissertations

The concept of literary homeplace serves as a locus of rhetorical healing for Black women, emphasizing their lived experiences and creative expressions as forms of resistance and restoration (Collins, 1986; hooks, 1990; Nettles, 2001). The labor of Black women within families, churches, and artistic endeavors contributes to the cultivation of a collective consciousness surrounding oppression and healing (Edwards, 2010; Carey, 2016; Morrison, 1992). Building upon hooks’ (1990) notion of homeplace, this dissertation examines how Black women foster spaces characterized by care, dignity, and resilience (Hersey, 2022; Tate, 1984). Using personal narrative and articulating Black women’s voices, I position homeplace as …

Enhancing Ai-Driven Automation For Object Detection And Computer Vision, Nafeeul Alam Walee

College of Graduate Studies: Theses & Dissertations

In recent years, AI-driven automation has revolutionized the field of object detection and computer vision, enabling sophisticated and efficient solutions across various industries. This research explores the latest advances and techniques in improving AI-driven automation for object detection and computer vision applications. We examine state-of-the-art deep learning models and frameworks that have contributed to significant improvements in accuracy and speed and highlight the generative results. The focus is on exploring the real-time processing capabilities that have expanded the applicability of these technologies in real-world scenarios. Furthermore, we investigate image integration and video data to improve precision detection and contextual understanding. …

Synthesis Of Tu100 Analogs And Biological Assays, Feyisope Tonye Oladapo

College of Graduate Studies: Theses & Dissertations

TU100 (14-methyl-5H-5,12-epiminobenzo[4,5]cyclohepta[1,2-b]naphthalene-6,11,13(12H)-trione) exhibits exceptional chemotherapeutic properties. It operates via a distinct mechanism that triggers cell death to create cytotoxic effects while concurrently inhibiting topoisomerase I and II. Its efficacy rivals that of well-known chemotherapeutic drugs like Daunorubicin. The distinctive characteristics of TU100 have led to initiatives aimed at creating structurally similar compounds to discover even more effective bioactive alternatives.

The analog synthesis incorporated quinoxaline or its derivatives (diphenyl quinoxaline, dipyridinyl quinoxaline, and dibenzophenazine-10,13-dione) that have been shown to have promising chemotherapeutic effects. Synthesizing the hetero naphthoquinones required a multi-step synthetic process, so quinoxaline/ derivatives intermediates are first prepared, then the …

Metalepsis And Mental Castration: Doki Doki Literature Club As The Cerebral Monstrous-Feminine, Hannah Graham

College of Graduate Studies: Theses & Dissertations

Doki Doki Literature Club! is a horror visual novel masquerading as a dating simulation game. Behind the thin veneer of pretty girls in tiny skirts and tight uniforms lurk horrors, glitches, and metaleptic events that take the player’s battle for agency beyond mere point-and-click interactions. This fight breaches containment to the level of the physical, as the player must literally wrestle for control with an in-game character who has commandeered their entire computer system. Players are led to believe that they are making the decisions that further the plot, but come to learn that in this world, there is no …

Journeys Of Love, Hope, And Possibility: Testimonios Of The Strength And Struggles Of Immigrant Latina Mothers Supporting Their Children's Educational Success, Mayra Endira Garcia-Diaz

College of Graduate Studies: Theses & Dissertations

This study explores the intersection of socio-cultural perspectives, cultural identity, and cultural motherhood involvement through the lived experiences of ten first-generation Latina immigrant mothers. The purpose is to analyze immigrant Latina mothers’ experiences, communications, and activities that support their children’s educational success. It fills the gaps in Latinx curriculum orientations by positioning cultural, emotional, and value-laden forms of learning at home as spaces of opportunity, places of possibility, and sites where ancestral wisdom meets everyday resistance. The study is grounded in testimonio as a Chicana/Latina feminist methodology and epistemology. Data were collected through in-depth semi-structured interviews conducted in Spanish to …

Assessment And Health Benefit Of Vitamin C Using A549 Cell, Yetunde Adepoju

College of Graduate Studies: Theses & Dissertations

Vitamin C (ascorbic acid) is a vital micronutrient recognized for its antioxidant capacity and essential bodily functions, such as collagen formation, neurotransmitter activity, and regulation of the immune system. This study explores how vitamin C can protect and potentially heal cells under oxidative stress and inflammation, using A549 cells, a human lung epithelial cell line, as the model system. The cells were exposed to varying concentrations of vitamin C dissolved in water, DMSO, and 5% DMSO. To assess the outcome, cell viability was measured with an MTT assay. In addition, the expression of key genes related to oxidative stress and …

Visual Attention And Memory Performance On Advertisements Featuring Celebrities, Kyrho Callis

College of Graduate Studies: Theses & Dissertations

VISUAL ATTENTION AND MEMORY PERFORMANCE ON ADVERTISEMENTS FEATURING CELEBRITIES

In Praise Of Dust, Hasler Gomez-Perez

Theses and Dissertations

A desire path is a physical manifestation of where a system has failed, but also where it has been refused, where a new path has been made. The wake exists in the post, it is not a singular, untethered event, but a subtle double entendre that lives in the post-death, but also in the pre-life—not a liminal limbo, but a superposition of dying and waking.

This collection was assembled and composed over the course of two years as I navigated my practice, myself, and others. Present here are my thoughts, my experiences, and in many ways my beliefs. IN PRAISE …

Facile Synthesis Of Gold, Copper, And Silicon Nanostructures By Laser Ablation In Liquid, Nicholas Simpson

Theses and Dissertations

Metal nanoparticle (NP) synthesis is a continually evolving area of research, owing to the widespread application of NPs in fields such as catalysis, drug delivery, and biosensing. Compared to bulk metal material, NPs contain a larger proportion of surface atoms and exhibit unique optical properties, thus making them ideal for applications where maximizing surface interactions is key. Moreover, their compositions can be altered in several different ways, as evidenced by metal alloy, core/shell, supported, and defective NPs. While conventional synthesis methods are capable of producing NPs in large quantities, they often require conditions of high temperature and pressure, strong reducing …

From Ink To Code, From Binary To Boundless Reality—Until All Returns To Emptiness, Weitong Sun

Theses and Dissertations

This thesis explores the entangled relationship between language, memory, and technology through the lens of immigrant experience. Moving from programming to visual storytelling, I investigate how dyslexia, bilingualism, and cultural displacement have shaped my ways of reading, writing, and coding.

Beginning with personal reflections on switching from computer science to art, I trace how code offered a form of clarity and expression where written language had once failed me. Yet as I ventured into real-time simulation and XR storytelling, I encountered new forms of mistranslation—where narratives dissolved, mutated, or resisted coherence altogether.

Through a series of experiments in code-driven art, …

How To Remain A Stranger / Encountering, In A Basement, Hannah Rotwein

Theses and Dissertations

How to Remain a Stranger / Encountering, in a Basement consists of two parallel essays. On the lefthand side of the page, How to Remain a Stranger explores the ways that the legend of the Wandering Jew, the Tradescantia genus of plants, peddlers, and exile relate to my practice of collecting, sorting, and combining found objects. On the righthand side, Encountering, in a Basement describes my thesis exhibition at the Institute for Contemporary Art at Virginia Commonwealth University. Focusing on the idea of the encounter, I detail the materials, considerations, and processes behind four of the ten sculptures in the …

A Parting Repast, Debra D. Dowden-Crockett

Theses and Dissertations

Both the thesis document and exhibition comprise the story of my journey through grief, examination of loss, contemplation of death, and transformation through acceptance and relinquishment. I conflate art and emotion to put form to the complex human experience of watching a loved one die, having to say a last goodbye, and the mourning that ultimately follows.

I examine the rituals surrounding death, the role of memory, and the symbolic presence of food as a shared experience of both mourning and nourishment, the intimate moments of mourning, and the gradual process of finding peace and closure.

Featuring works from a …

Post Opera, Suzy Lykins

Theses and Dissertations

Post Opera is a spell and work of embodied research which explores medicalization, desire, labor, and belief. Through methods of performance, sound, text and installation, I facilitate encounters of trans-femininity, spiritual power and connectivity. An opening wound, a set of psychic scalpels, sutures and bandages prepare this text for your consumption, take care to digest her well.

The Paradox Of Value, David Enrique Guarnizo

Theses and Dissertations

The present thesis project examines how, in the United States, and especially in the state of Virginia, archetypes of "wealth," "value," and "identity" have been constructed in relation to land and territory. This analysis explores the tension between the inherent value of life on the land and the economic value that can be derived from it. This tension is exemplified by a theory known as the "paradox of value", also referred to as the "paradox of water and diamonds", which was developed by the renowned Scottish economist and philosopher, Adam Smith. This theoretical framework serves as a fundamental axis for …

Dosimetric Validation Of An Electron Monte Carlo (Emc) Algorithm For Small Cutouts And Extended Ssd’S: Assessing Emc In Small Field Dosimetry, Anil Basavaaraju

Theses and Dissertations

This thesis validates the accuracy and limitations of Electron Monte Carlo (eMC) simulations in Eclipse treatment planning systems for small-field electron dosimetry and extended source-to-surface distances (SSDs). Systematic measurements were performed using a microDiamond detector across various electron energies and field sizes. The study examines the discrepancies between measured and calculated (eMC) dose distributions, validating challenges due to lateral scatter disequilibrium and extended air gaps. Our findings provide systematic data for eMC dose calculations and demonstrate that eMC performs within clinical tolerances, with the ultimate goal of enhancing the precision and safety of radiation treatments.

Feeling Foreign / Foreign Feeling: Affect In Southeast Asian Diasporic Short Story Collections, Francys Aerin Fortes

Theses and Dissertations

Affect studies have long been saturated with scholarship investigating the fluidity of emotions, though a larger turn in the field now prioritizes the felt experiences of marginalized peoples. Sianne Ngai’s work is one part of this turn that seeks to unpack the effects of oppressive systemic forces on Black, Brown, and queer bodies. Sianne Ngai reexamined the nature of emotions in her seminal text Ugly Feelings (2005), particularly concerned with how negative feelings are disproportionately felt by marginalized bodies as a result of conditions of obstructed agency, systems of power, and wage labor capitalism. Drawing from Ngai’s writing, this thesis …

Through The Lens Of Social Media: Examining The Relationship Between Exposure To Social Media Videos Of Police Misconduct And Perceptions Of Legitimacy And Cooperation With Campus Police, Kamal Alhassan

Theses and Dissertations

This dissertation examines how exposure to social media and internet news videos depicting police misconduct influences college students' perceptions of campus police legitimacy, procedural justice, and willingness to cooperate. Although prior research has demonstrated the impact of media portrayals on perceptions of municipal police, limited attention has been given to campus policing contexts. Guided by Tyler’s process-based theory of police legitimacy and Gerbner’s cultivation theory, this quantitative study surveyed 400 students at a large public university in the Southeastern Mid-Atlantic region. Measures included self-reported viewing and engagement with police misconduct content on social media, internet, and traditional media platforms, as …

Beneath The Roots: Becoming Native Through Mangrove Teachings And Wisdom, Shima Aeinehdar

Theses and Dissertations

Beneath the Roots uses the wisdom of mangroves to investigate the naturalization of humans through science, spirituality, and storytelling. The seeds of Avicennia marina, the gray mangrove, drifted to Qatar at some point in history and took root, like so many other people here. The mangrove survives in a harsh saline environment through a unique salt excretion process. As a Qatar-born expat, I relate to this struggle to survive and nativize in a land that is both my own and foreign to me, in a cyclical conversation. I explore this nativization process through a ritual of salt extraction, creating botanical …

Imagine Being Free: Decolonizing Actor Training In University And Conservatory Programs, Hope R. Ward

Theses and Dissertations

While blackface is considered the first proper form of American theater, I want to consider how the auction of enslaved Africans functioned as the original form of American theater. The auction block acted as a kind of interactive performance in which enslaved bodies were surveilled and evaluated as a kind of spectacle. In many ways, the auction block inaugurated White viewing practices of the Black body that reverberated in the day-to-day practices of slavery and subsequent visual media like the refashioning of blackface minstrel tropes within contemporary entertainment (plays, television, film). In Imagine Being Free: Decolonizing Actor Training in University …