Digital Commons Network^™

Development And Applications Of Capillary Vibrating Sharp-Edge Spray Ionization Coupled With Atmospheric Pressure Chemical Ionization (Cvssi-Apci), Madison Elizabeth Pursell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mass spectrometry (MS) is a technique that is used in fields in which both complex and non-complex mixtures are analyzed. The state-of-the-art ion sources that are utilized for MS are being demonstrated to be functional for direct infusion of samples as well as for use with online separations. However, ion sources, such as electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) can encounter problems such as ion suppression and an inability to ionize based on size and/or polarity, respectively. Further these sources are bulky and require gas flow, heat sources, and additional high voltage power supplies for optimal performance. …

Decoding Tattoo And Permanent Makeup Pigments: Linking Physicochemical Properties To Absorption, Distribution, Metabolism, And Elimination Profiles Using Quantitative Structure-Activity Relationship (Qsar)-Based New Approach Methodologies (Nams), Girija Bansod, Ajay Vikram Singh, Preeti Bhardwaj, Tulika Rai, Sweta Vijay Nakhale, Amruta Shelar, Rajendra Patil, Peter Laux, Andreas Luch, Christopher J. Osgood, Michael W. Stacey

Biological Sciences Faculty Publications

The safety and quality of tattoo and permanent makeup (PMU) pigments are subject to increased scrutiny due to their potential to cause adverse effects like anaphylaxis, photoallergic responses, and long-term toxicity. These undesirable reactions governed by their chemical structure possess varied physicochemical properties and absorption, distribution, metabolism, and elimination (ADME) characteristics. These properties control the pigment behavior during application, stability, and interaction with human tissue. The correlation between these physicochemical characteristics and ADME parameters of tattoo/PMU pigments remain under-explored despite the current advances in toxicology. Our study aims to address and bridge the gap by leveraging open-access QSAR computational toxicology …

Comparative Study Of Single Imputation Techniques For The Prediction Of Missing Dairy Data, Ahmed M. Gad Prof, Ahmed Abdelhakim Ahmed Mr, Eman Manaa Prof, Basant Shafik Dr, Sakr Mostafa Prof

Business Administration

Dairy farm records are a crucial component of effective livestock business management. Record analysis allows a farm’s owner to make informed decisions. Incomplete records are less useful for data analysis, so it's important to handle missing values correctly. This study compares different imputation methods for handling missing values in a dataset of dairy records comprising 997 records collected from 234 cows between 2012 and 2022. The dataset was screened against records with missing values and then deleted, resulting in 858 observations from 200 animals. There were missing values in two variables, with a missing percentage of 13.9%: days in milk …

Advancing Root Trait Measurements For A Deeper Understanding Of Soil Biogeochemistry, Brian Rinehart

Theses and Dissertations--Plant and Soil Sciences

Climate change is disrupting (agro)ecosystems, with the potential for significant losses in crop productivity and ecosystem resilience due to changes in water and biogeochemical cycling. These disruptions can also release more carbon dioxide into the atmosphere through the degradation of soil organic matter. Plant roots have emerged as a potential tool for mitigating these effects, given their roles in plant function and in driving soil biogeochemistry. While this interest is growing, our ability to implement root trait selection is limited due to constraints on characterizing roots and limited clarity on the effects of traits on plant and ecosystem function. My …

Establishing A Conventional Linac-Based Electron Flash Beam, Justin Defrancisco

Theses and Dissertations

Problems: A rediscovered radiotherapy technique, FLASH, involves increasing the dose rate to >40 Gy/s. For a given dose, FLASH is shown to decrease the normal tissue complication probability without affecting the tumor control probability. More preclinical studies are necessary to confirm and enable these desirable properties on humans. Current preclinical investigations have diverse constraints, requiring FLASH apparatuses with flexible parameters and accurate control. However, there are limited FLASH platforms available, the existing ones are costly. Our institution is not in possession of any such infrastructure. Even if available, dosimetry and beam control in FLASH are extremely lacking. There is …

Nature, Nurture, And Serial Murder: A Biocriminological Analysis Of Jeffrey Dahmer And Aileen Wuornos, Dori W. Jal

Honors Thesis

In recent years, there has been an increase in public fascination with serial killers, however current criminological explanations continue to focus on social and psychological factors, lacking commentary on potential biological implications. This thesis addresses a gap in the literature by examining how biological processes, specifically epigenetic, neurobiological, and endocrinological mechanisms, interact with environmental stressors to shape serial homicidal behavior. The goal is to determine whether serial murder can be more effectively understood through a biosocial framework that integrates biological vulnerability with lived experience, rather than relying on singular explanatory models.

Using a qualitative multiple-case study methodology, this research analyzes …

Raising Information Literacy Of Biology Pre-Service Teachers: Study On Three Problem Solving Methods, Muhammad Saefi, Hadi Suwono, Rifka Fachrunnisa, Widi Cahya Adi, Herawati Susilo, Ahmad Kamal Sudrajat

Australian Journal of Teacher Education

Integrating information literacy teaching methods with subject-content curricula to develop prospective biology teacher students' abilities is still problematic. This quasi-experimental research, with a nonrandomized pretest-posttest control group design, intends to compare the influences of challenge-based learning (CBL), problem-based learning (PBL), and problem-solving through classroom discussion (PCD) – as the control- in improving information literacy. This study involved 183 students from three classes of freshmen and three sophomores in the biology education program. The results showed that CBL significantly improved students' information literacy compared to PBL and PCD across five indicators: 1) identifying the required information, 2) attaining the required information …

Defining Dysphoric Milk Ejection Reflex: Using Premenstrual Dysphoric Disorder As A Framework For Proposing Preliminary Diagnostic Criteria, Megan Howard, Teni Davoudian, Nicole H Cirino

Faculty, Staff and Students Publications

Dysphoric Milk Ejection Reflex (D-MER) is a distinct neurobiological condition characterized by negative alterations in mental state in response to milk letdown during lactation. Symptoms vary by patient and can include feelings of sadness, anxiety or agitation. Importantly, the symptoms are brief, typically lasting no more than 5 minutes. Prevalence has been found between 6 and 27% of lactating women, but studies show heterogeneity, due in part to inconsistent definition. D-MER is not currently classified in the Diagnostic and Statistical Manual of Mental Disorders (DSM) or the International Classification of Diseases (ICD), which presents a challenge for researchers of the …

Inorganic Arsenite [I-As (Iii)] Represses Human Renal Progenitor Cell Characteristics And Induces Neoplastic-Like Transformation, Md Ehsanul Haque

Theses and Dissertations

Studies on populations exposed to inorganic arsenic (iAs) have shown an association between this particular exposure and the development of chronic kidney disease (CKD) and renal cell carcinoma (RCC). However, there are few studies addressing how acute exposure of the human kidney to iAs might lead to long-term alterations that might lead to CKD or RCC. This study’s hypothesis is that renal exposure to iAs might alter the renal cells responsible for the repair and regeneration of nephrons damaged by iAs exposure or other renal toxicants. The kidney possesses a minority epithelial cell population that co-express PROM1 and CD24 that …

Genome-Wide Snp Analyses Reveal High Gene Flow Of Endemic Smallscale Croaker (Boesemania Microlepsis) In The Lower Mekong Basin, Quyen Dang Ha Vu, Christopher E. Bird, Oanh Thi Truong, Sang Quang Tran, Ellen E. Biesack, Amanda S. Ackiss, Kent E. Carpenter, Binh Truy Dang

Biological Sciences Faculty Publications

The Smallscale Croaker, Boesemania microlepis, is a valuable fishery resource in the Mekong River basin that lacks clear biological data to understand its basic ecology and conservation management priorities. This species is common in the southernmost floodplain of the Mekong which extends from Tonlé Sap in Cambodia to the Mekong Delta in Vietnam. This floodplain is of particular biodiversity conservation concern because of the many upstream dams that restrict water flow in an ecology that relies heavily on a flood-pulse cycle. The literature regarding the biology B. microlepis in this region contains conflicting ideas that populations are both highly …

Sulfur Chemistry For Protein Synthesis And Modification: From Thiolactone To Bicyclic Sulfoxide Frameworks, Yuming Wen

Electronic Theses & Dissertations (2024 - present)

This dissertation explores two intersecting themes in modern chemical biology: (1) the design of novel ligands for transition-metal catalysis, and (2) the development of thiolactone-mediated strategies for protein synthesis and modification in aqueous media. Together, these studies advance chemical methods for precise molecular transformations under mild, biocompatible conditions.

In the first chapter, a geometrically constrained bicyclic [3.3.1] sulfoxide framework was developed as a new class of bissulfoxide ligands for palladium-catalyzed C–H functionalization. The ligand was synthesized concisely from thiophenol through a three-step sequence and shown to promote allylic C–H amination and alkylation reactions with high efficiency. The catalytic system tolerated …

I Get It From My Momma: Exploring The Process Of Transgenerational Resiliency Among Two Generations Of Single Black Mothers, Destiny A. Bender

Electronic Theses & Dissertations (2024 - present)

Abstract

Aim: This dissertation examines how resilience is transmitted across two generations of Black women (18 to 70 years of age). It aims to enhance our knowledge of the meaning of resilience in the context of single motherhood and how beliefs, values, faith-based approaches to coping, and culturally driven approaches to mothering influence outcomes for the next generation. Knowledge gaps regarding theoretical formulations of resilience and single Black mothers are discussed. Methodology: The researcher conducted in-depth interviews with ten women (five mother-daughter dyads). A phenomenological inquiry was conducted to explore the meaning-making process of resilience for mothers; human development, ecological …

Modelling The Impacts Of Recreational Catch-And-Release Variability On Fish Stock Dynamics, Brett Crisafulli

Theses: Doctorates and Masters

Recreational fisheries often lack sufficient data for robust stock assessments, and post-release mortality (PRM) of discarded fish, particularly demersal species, poses a significant threat to sustainability. Because demersal fish often experience higher PRM (often due to barotrauma and stress), the substantial global interest in their recreational fisheries (e.g., Australia, Gulf of Mexico, Western Baltic Sea) raises conservation concerns. This study employs three distinct modelling approaches, applied to four reef-dwelling demersal species, to support effective recreational fisheries management by addressing data uncertainty, unintended consequences of management changes, and PRM effects. Specifically, this thesis (1) evaluates variability in average weights of recreationally-caught …

Corchorus Olitorius Exhibits Antiproliferative Potential Supported By Metabolic Profiling And Integrative Biological Analyses, Maha R.A. Abdollah, Salma Sameh, Ahmed Elissawy, Eman Al-Sayed, Rola Labib, Lan Ye, Fang-Rong Chang, Abdel Nasser B. Singab

Pharmacy

Herbal nutraceuticals could be employed as alternative or complementary routes for alleviating cancer. Corchorus olitorius (Malvaceae) was employed traditionally in the management of tumors. The study aimed to investigate the antiproliferative activity of C. olitorius leaves. In vitro cytotoxic and anti-angiogenic activities of C. olitorius were estimated. The bioactive fraction was subjected to in vivo study on BALB/ c female mice using Ehrlich Ascites Carcinoma model. UPLC-ESI-MS/MS analysis was done to determine the phytometabolites followed by in silico studies on the major identified compounds. The bioactive fraction possessed potent in vitro activity against A549 cells with IC50 = 7.8 µg/mL …

Heterogeneous Clustering Of Multiomics Data For Breast Cancer Subgroup Classification And Detection, Joseph Pateras, Musaddiq Lodi, Pratip Rana, Preetam Ghosh

Computer Science Faculty Publications

The rapid growth of diverse -omics datasets has made multiomics data integration crucial in cancer research. This study adapts the expectation–maximization routine for the joint latent variable modeling of multiomics patient profiles. By combining this approach with traditional biological feature selection methods, this study optimizes latent distribution, enabling efficient patient clustering from well-studied cancer types with reduced computational expense. The proposed optimization subroutines enhance survival analysis and improve runtime performance. This article presents a framework for distinguishing cancer subtypes and identifying potential biomarkers for breast cancer. Key insights into individual subtype expression and function were obtained through differentially expressed gene …

Optical Methods For Time-Resolved Dosimetry And Oximetry Of Ultra-High Dose Rate Radiation Therapy, Megan A. Clark

Dartmouth College Ph.D Dissertations

Radiotherapy (RT) is a cornerstone method used to treat over 50% of the 2 million new cancer diagnoses each year in the United States. The success of RT directly relies on an optimal balance between maximizing the dose to the tumor while minimizing dose to surrounding normal tissues. Achieving this balance is often challenging due to underlying radiation transport and the presence of anatomical constraints that limit the beam delivery geometry. In turn, minimizing healthy tissue toxicity prevents use of a more aggressive tumor killing approach, and even in curative cases may decrease the patient’s quality of life due to …

Parasitism By The Twisted-Winged Parasite Xenos Peckii (Strepsiptera), And Its Effects On The Ovary Development In Their Host Paper Wasp, Polistes Metricus (Hymenoptera), Alex K. Snyder

College of Graduate Studies: Theses & Dissertations

Strepsiptera are generally described as parasitic castrators as they cause the reproductive death of the hosts. In southeast Georgia, only a handful of paper wasps are hosts for twisted-winged parasites. One such species, Polistes metricus, is parasitized by a twisted-winged parasite, Xenos peckii. Previous studies document that host survivorship at most developmental stages was not largely influenced by the presence of X. peckii. To determine if X. peckii can affect their host’s ability to survive and reproduce, we dissected P. metricus to determine ovary development and the presence of X. peckii parasites. Parasite presence had no effect on the host’s …

Ajwa Date Extract (Phoenix Dactylifera L.): Phytochemical Analysis, Antiviral Activity Against Herpes Simplex Virus-I And Coxsackie B4 Virus, And In Silico Study, Ahmad K. Aljohani, Nader A. Maghrabi, Osama M. Alrehili, Abdulaziz S. Alharbi, Rawad S. Alsihli, Abdulrahman M. Alharthe, Rayan S. Albladi, Khalid A. Alosaimi, Bader M. Albadrani, Samar F. Miski, Hossein M. Elbadawy, Bandar D. Alrehaili, Fahd A. Abdelkarem, Modather F. Hussein

Saudi Medical Journal

Objectives: To investigate the phytochemical composition of Ajwa date extract and evaluate its antiviral activity and mechanism of action. Methods: High perfomance liquid chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry were used to analyze the phytochemical profile of Ajwa date extract. The antiviral activity was assessed using the MTT colorimetric assay against herpes simplex virus type I (HSV-I) and coxsackievirus B4 (CVB-4). Assessment of the mechanism of action against HSV-I was carried out using 3 protocols. Molecular docking and quantum chemical calculations were carried out to predict the binding affinities of the identified compounds to viral glycoprotein D. Results: …

Quantifying Avian And Vegetation Communities On Agricultural Conservation Easement Wetlands In Eastern South Dakota, Katherine Millman

Electronic Theses and Dissertations

The Prairie Pothole Region of eastern South Dakota contains numerous small depressional wetlands, colloquially known as potholes, that provide important ecosystem services, including water storage, water quality improvement, and wildlife habitat. The Agricultural Conservation Easement Program (ACEP) was established by the U.S. Department of Agriculture to protect and restore wetlands and grasslands on private land through easements such as Wetland Reserve Easements. Although ACEP requires management practices intended to maintain or improve habitat quality, few studies have evaluated their effectiveness at supporting wildlife communities and vegetation integrity. To address this gap, we evaluated wetlands at 30 ACEP sites and 20 …

An Integrated Wetland Condition Index And Environmental Metric Framework For The Prairie Pothole Region, Mercedes L. Batalla

Electronic Theses and Dissertations

The USDA-NRCS Agricultural Conservation Easement Program (ACEP) assists landowners with protecting, restoring, and enhancing wetlands. Evaluating wetland conservation efforts is crucial for informing future management decisions. However, assessment frameworks vary across regions and among stakeholders. The Prairie Pothole Region is an ecologically unique landscape characterized by its abundance of wetlands that provide important ecosystem services. To evaluate wetlands enrolled in ACEP, I sampled forty-five semi-permanent wetlands in the Prairie Pothole Region from July – August 2023 and 2024. I selected and sampled twenty-six wetlands enrolled in ACEP. Nineteen additional wetlands not enrolled in ACEP were also sampled; these included nine …