Digital Commons Network^™

Folate Receptor Β Performs An Immune Checkpoint Function In Activated Macrophages, Fenghua Zhang, Md Yusuf Al-Amin, Sagar Utturkar, Rina Jiang, Gregory Cresswell, Rami Alfar, Ian Ophaug-Johansen, Gabriel Bachman, Madduri Srinivasarao, Richard Finnell, Amaya Puig-Kröger, Timothy Ratliff, Philip S Low

Faculty, Staff and Students Publications

Monocytes and macrophages are sentinels of the immune system that distinguish themselves from other cells by expressing the beta isoform of the folate receptor (FRβ). Because FRβ does not bind folate until the monocyte/macrophage is exposed to immunosuppressive cytokines, the question naturally arose whether FRβ might also perform an immune-related function. To examine this matter, we compared the properties of wild type (WT) and FRβ knockout mice. We observe that FRβ knockout (KO) mice display autoimmune symptoms that can include alopecia, enlarged spleens, and dermatitis, despite having normal cellular folate levels. We further demonstrate that syngeneic tumors (TRAMP C2, MC38) …

A Murine Model Of Gestational Diabetes Reveals Masld Risk And Alterations In Markers Of Hepatic Mitochondrial Metabolism, Grace E Shryack, Alexa A Krause, Simone Hernandez Ruano, Laura C Schulz, Kathleen A Pennington, R Scott Rector

Faculty, Staff and Students Publications

Introduction: Gestational Diabetes Mellitus (GDM) impacts roughly 1 in 7 pregnancies and results in metabolic dysfunction-associated steatotic liver disease (MASLD) in 30% of these women. Nonetheless, there exists a dearth of investigation into the relationship between GDM and MASLD. Here, we sought to investigate the potential role of hepatic mitochondrial function in GDM and MASLD.

Methods: One week prior to conception and throughout pregnancy, mice were fed either a low-fat control diet (CD) or a high-fat, high-sucrose (HFHS) diet to induce an established model of GDM. Maternal livers were collected at day 0, 6.5, 13.5 and 17.5 of pregnancy. Hepatic …

The Line: Ai And The Future Of Personhood By James Boyle: Reviewed By Hannah Drayson, Hannah Drayson

School of Art, Design and Architecture

No abstract provided.

Nitrate And Nitrite Bioavailability In Plasma And Saliva: Their Association With Blood Pressure - A Systematic Review And Meta-Analysis, Raul Bescos, Laura Gallardo-Alfaro, Ammar Ashor, Lucia Rizzolo-Brime, Mario Siervo, Patricia Casas-Agustench

School of Health Professions

In this study, we conducted a systematic review and meta-analysis to determine plasma and salivary nitrate (NO ₃ ⁻) and nitrite (NO ₂ ⁻) concentrations under resting and fasting conditions in different type of individuals and their association with blood pressure levels. A total of 77 studies, involving 1918 individuals aged 19–74 years (males = 906; females = 1012), which measured plasma and/or salivary NO ₃ ⁻ and NO ₂ ⁻ using the chemiluminescence technique, were included. Mean plasma NO ₃ ⁻ and NO ₂ ⁻ concentrations were 33.9 μmol/L and 158.3 nmol/L, respectively. Subgroup analyses revealed lower …

Leveraging Human Microbiomes For Disease Prediction And Treatment, Henok Ayalew Tegegne, Tor C Savidge

Faculty, Staff and Students Publications

The human microbiome consists of diverse microorganisms that inhabit various body sites. As these microbes are increasingly recognized as key determinants of health, there is significant interest in leveraging individual microbiome profiles for early disease detection, prevention, and drug efficacy prediction. However, the complexity of microbiome data, coupled with conflicting study outcomes, has hindered its integration into clinical practice. This challenge is partially due to demographic and technological biases that impede the development of reliable disease classifiers. Here, we examine recent advances in 16S rRNA and shotgun-metagenomics sequencing, along with bioinformatics tools designed to enhance microbiome data integration for precision …

Poststroke Impulsivity: A Narrative Review, Melissa B Jones, Dakota E Broadway, Malena Gimenez-Zapiola, Ricardo E Jorge

Faculty, Staff and Students Publications

In this narrative review, the authors examine the multidimensional nature and presentations of impulsivity after stroke. Impulsivity manifests as immediate or premature responses, impaired delayed gratification, perseverance despite punishment, and other displays of impaired emotional and behavioral regulation. The literature on the assessments, outcomes, and treatment of patients with these various manifestations after a cerebrovascular injury is reviewed. Findings from case reports indicate that poststroke impulsivity may manifest across neurobehavioral syndromes that are not well defined in the psychiatric nomenclature, such as alien hand syndrome and atypical impulse control disorders. Overall, impulse control disorders appear to be rare poststroke. Therapeutic …

Anatomical Depiction: How Showing A Product's Inner Structure Shapes Product Valuations, Seo Yoon Kang, Junghan Kim, Arun Lakshmanan

Research Collection Lee Kong Chian School Of Business

Anatomical depiction is a technique where the product is decomposed into components that are spatially arranged in a layer-by-layer manner to visually explicate its inner structure. The authors demonstrate that anatomical depiction, compared to non-anatomical depiction, enhances product valuation. This effect occurs because anatomical depiction elicits a ‘coming together’ of the inner components in consumers’ minds thereby evoking a gestalt image of the product – a process labeled simulated assemblage. The elicitation of simulated assemblage in turn boosts their confidence in the product’s performance. Two field experiments first demonstrate that anatomical depiction leads to greater engagement in online settings such …

Collective Memory For The African Motherland In Interwar Black Chicago And The Limits Of The Environmental Justice Model, Colin Fisher

History: Faculty Scholarship

No abstract provided.

Transgenerational Plasticity Research: Challenges And Opportunities, Caitlin Mcandry, Alastair J. Wilson, Peter A. Cotton, Manuela Truebano

School of Biological and Marine Sciences

Transgenerational plasticity (TGP) is a type of non-genetic inheritance whereby one generation's environment affects a subsequent generation's phenotype. It can influence the persistence of organisms in stressful climatic conditions through allowing rapid phenotypic change. TGP has been proposed to have the potential to alter an organism's response to selective forces or buffer their effects, yet the interaction of these two processes is neither well understood nor often accounted for via appropriate experimental design. The rapid expansion of TGP research in recent years has led to a lack of clarity surrounding terminology for different phenomena, sometimes undermining the interpretation of research. …

Engineering Nanomaterials From Phase-Change Biological Materials Reacting With Carbon Dioxide, Qingyang Li

Graduate Theses, Dissertations, and Problem Reports (ETD)

The current global situation concerning CO2 emissions is increasingly alarming, with rising concentrations of CO2 significantly worsening climate change and global warming. Atmospheric CO2 levels are now at their highest in millions of years, driving a host of catastrophic effects, including but not limited to extreme weather events, rising sea levels, ocean acidification, disrupted ecosystems, deteriorating air quality, respiratory health issues, heat-related illnesses, threats to food and water security, and escalating social and geopolitical tensions. To keep global warming to no more than 1.5°C higher than that of pre-industry – as called for in the Paris Agreement – emissions need …

Improving Food Safety Practices Among Fsma-Exempt Small-Scale Produce Growers: A Manuscript-Based Dissertation On Risk, Adoption, And Practical Barriers, Jesica Marie Temple

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Improving Food Safety Practices Among FSMA-Exempt Small-Scale Produce Growers: A Manuscript-Based Dissertation on Risk, Adoption, and Practical Barriers

Jesica Temple

Foodborne illness outbreaks associated with fresh produce continue to pose a public health risk in the United States. Although the Food Safety Modernization Act (FSMA) Produce Safety Rule (PSR) established science-based standards to prevent contamination, many small-scale farms are exempt from these requirements. Consequently, voluntary adoption of Good Agricultural Practices (GAP) varies widely across regions. Strategies to improve engagement among FSMA-exempt growers are key to advancing postharvest food safety and reducing contamination. This manuscript-based dissertation examined how knowledge, attitudes, …

Using Algal Turf Scrubber Biomass As A Feed Source For Juvenile Eastern Oysters, Taofeek Saka Jimoh

College of Graduate Studies: Theses & Dissertations

Algal turf scrubber (ATS) is an engineered system that promotes natural wastewater treatment processes and improves water quality by passing a shallow stream of nutrient rich water over the surface of a gently sloped flow-way. Algae turf scrubber biomass is a combination of micro and macroalgae, as well as micro-invertebrates, ciliates, bacteria, and more. The overall aim of the study was to reduce the feeding cost of juvenile eastern oysters by replacing the commercial microalgae diet with ATS biomass. The experiment was designed to assess the viability of replacing commercial microalgal diet with ATS biomass at different substitution levels (0, …

It’S A Slippery Slope: A Journey Of Questioning Religion And Building Homeplace, Emily Richardson-Dion

College of Graduate Studies: Theses & Dissertations

Homeplace (Whitlock, 2007) describes the place where we live most comfortably. It is not confined to a physical place, but is constructed by the culture, customs, beliefs, and messages, implicit and explicit, that we absorb over the course of our lives. In this study, I utilize the method of currere, a method of self-study established and then reimagined by Pinar (2023), and Critical Race Feminist Currere introduced by Baszile (2015) to examine my educational experiences and more fully understand what has contributed to my personal construction of homeplace. My experience, like that of Baszile (2015), reflects the need to examine …

Assessing The Impact Of Environmental Health Risk Communication On Cancer Screening Intentions Among Private Well Owners In Rural Communities, Victoria A. Clower

College of Graduate Studies: Theses & Dissertations

Background: Cancer is a leading cause of death in Georgia, with rising incidence in rural counties where many residents rely on unregulated private wells. These wells may contain contaminants such as radon, lead, nitrate, and arsenic, all linked to increased cancer risk. Limited research has explored how environmental exposure awareness influences cancer prevention behaviors such as screening. Guided by the Champion Health Belief Model, this study examined psychosocial and environmental predictors of screening intent among private well owners. Methods: Well Aware was an 11-week quasi-experimental study among 60 private well owners in southeast Georgia. Participants who attended educational listening sessions …

A Critical Examination Of Scent Avoidance And Olfactory Development After Neonatal Predator Scent Exposure, Cynthia Ware

College of Graduate Studies: Theses & Dissertations

The long-standing assumption that predator odor avoidance in rodents is an innate survival mechanism has shaped decades of behavioral neuroscience research and underpins translational models of post-traumatic stress disorder (PTSD). However, developmental systems theory (DST) posits that behaviors emerge from dynamic interactions across physiological and environmental systems over time, rather than from fixed genetic programs. This study critically evaluates the developmental plasticity of predator scent aversion by examining whether early-life exposure to red fox urine (a natural predator odor) can attenuate avoidance behaviors in Long–Evans rats. Seventy-one rats were bred into three conditions: Control (no exposure), Partial (exposure from birth), …

An Introduction To U.S. Groundwater Law: Domestic And Transboundary Considerations, Caleb Cook, Gabriel Eckstein

Faculty Scholarship

In light of the scientific and historical factors that have shaped the development of groundwater law and policy, this chapter seeks to provide a primarily descriptive account of its current state in the United States and to lay a foundation for future efforts to address gaps in the regime. Section 8B.02 considers the importance of groundwater in modern American society, with a particular focus on its uses and the threats these resources face. Section 8B.03 describes the U.S. framework for the domestic governance of groundwater resources. Section 8B.04 describes the mechanisms for transboundary governance of groundwater between the United States …

"Can We See How It Works?" The Interactions Among Teacher, Student, And Content At A Living History Museum, Stacy E. Ward

Electronic Theses & Dissertations (2024 - present)

Today’s students need to be critical consumers of information. To that end, a thoughtful social studies curriculum provides students an opportunity to gain content knowledge and to practice thinking skills. However, elementary teachers, especially primary grade (K-2) teachers, often do not possess the skills and content knowledge to teach rich historical content and thinking skills. Teachers can partner with museum professionals and access museum content in this endeavor. Living history museums, immersive museums that interpret multiple buildings, trades, and heritage breed animals, hold the potential to be a strong partner for primary grade students, who are only 5-, 6- and, …

Epithelial Responses And Remodeling In The Submandibular Gland Post-Irradiation, Victoria Gellatly

Electronic Theses & Dissertations (2024 - present)

Radiation therapy for head and neck cancer often causes irreversible salivary gland dysfunction, resulting in chronic xerostomia. Understanding the cellular and molecular mechanisms underlying this dysfunction is essential for developing regenerative therapies. Given the cellular complexity of the salivary gland single-cell RNA sequencing (scRNA-seq) provides a powerful approach to investigate cell-type-specific injury responses to ionizing radiation (IR) and uncover transcriptional changes that may be masked in bulk analyses. This study investigates the cellular and molecular responses of the murine submandibular gland (SMG) to irradiation, with a focus on epithelial loss and remodeling, and regenerative failure. In order to effectively use …

Reconstructing The Meaning Of "Forty Acres And A Mule", Cynthia Nicoletti

Faculty Scholarship

This article offers a new interpretation of the land grant to freedpeople contained in General William T. Sherman’s Special Field Orders No. 15. Issued in January 1865 at the end of the Civil War, Sherman’s Orders are widely considered to be the origin of the government promise to endow freedpeople with “forty acres and a mule” as they transitioned from slavery to freedom. As such, the Special Field Orders have played a central role in shaping the cultural memory of the Civil War, historical accounts of Reconstruction, and contemporary debates over reparations for slavery. They have also been misunderstood and …

Affording Oral Tradition Evidence Due Weight Before The International Court Of Justice, Terrence Neal

Faculty Scholarship

Oral traditions are irreplaceable sources of historical information, particularly with regard to Indigenous Peoples’ histories and cultures. However, when states have submitted oral traditions as evidence of historical practices, events, and circumstances in cases before the International Court of Justice (“Court”), the Court has been reluctant to afford them significant weight. This Article, which is the first detailed study on the Court’s treatment of oral traditions, examines whether the Court is failing to provide them due or appropriate weight. Based on historical, archaeological, and anthropological research, it establishes that oral traditions can be as reliable as written documents for reconstructing …

Glutamatergic Lateral Habenula Neurons Modulate Consolidation Of Associative Memories, Snigdha Srivastava, I-Ching Wang, Mikhail Y Kochukov, Jessica L Swanson, Mauro Costa-Mattioli, Benjamin R Arenkiel

Duncan NRI Faculty and Staff Publications

INTRODUCTION: Despite the rise in psychiatric disorders worldwide, the underlying brain circuits responsible for these devastating conditions remain elusive. The lateral habenula (LHb) has emerged as a key brain structure in depression studies due to its hyperactive state in both patients and animal models. While this aligns with known roles in driving aversive states and regulating serotonin release, it is still unclear how acute and transient activity changes in the LHb can influence higher order cognitive processes such as learning, memory, and behavioral adaptation. Given the importance of these processes to psychiatric conditions, understanding how LHb activity impacts cognitive function …

Drosophila Models Uncover Substrate Channeling Effects On Phospholipids And Sphingolipids In Peroxisomal Biogenesis Disorders, Michael F Wangler, Yu-Hsin Chao, Mary Roth, Ruth Welti, James A Mcnew

Duncan NRI Faculty and Staff Publications

Peroxisomal Biogenesis Disorders Zellweger Spectrum (PBD-ZSD) disorders are a group of autosomal recessive defects in peroxisome formation that produce a multi-systemic disease presenting at birth or in childhood. Well documented clinical biomarkers such as elevated very long chain fatty acids (VLCFA) are key biochemical diagnostic findings in these conditions. Additional, secondary biochemical alterations such as elevated very long chain lysophosphatidylcholines are allowing newborn screening for peroxisomal disease. In addition, a more widespread impact on metabolism and lipids is increasingly being documented by metabolomic and lipidomic studies. Here we utilize Drosophila models of pex2 and pex16 as well as human plasma …

Poor In Practical Capacity: How Environmental Alienation Is Really A Deficit Of Political Know-How, Jeremy Bendik-Keymer

Faculty Scholarship

“Alienation from nature” is a popular notion in Western environmental culture. Influential Anglophone critical theorist Steven Vogel claims that it makes no sense, unlike alienation from our productive capacity to dwell on Earth, called “alienation from the environment.” His criticism is accurate, but his view isn’t. The normative sets appropriate production and consists of social processes of arriving at norms. Politics is foremost among these processes, and it is fundamentally know-how. Given these assumptions, poor practical capacity ends up being the heart of “environmental alienation” – alienation from the built environment. Look at large-scale, anthropogenic, environmental change: a deficit of …

Evaluating Impacts Of The Trichloroethylene Metabolite S-(1,2-Dichlorovyinyl)-L-Cysteine On Transcriptomic Responses And Cytokine Release In A Macrophage Model: Implications For Pregnancy Outcomes, Annella Benjamin, Erica Boldenow, Sammy Jaber, Kelly M. Bakulski, John Dou, Justin Colacino, Peter Mancuso, Sean Harris

University Faculty Publications and Creative Works

Trichloroethylene (TCE) is a volatile synthetic chemical used in various industrial processes like metal degreasing. Large amounts of TCE have been released into the environment. Exposure to TCE can occur through routes, such as inhalation for workers using TCE or ingestion of drinking water in contaminated areas. Macrophages are key immune cells in virtually all tissues in the human body, including the fetal membranes, making them a plausible target for DCVC-induced immunotoxicity. Macrophages are critical for maintaining anti-microbial defenses during pregnancy, but little data exists on TCE immunotoxicity during pregnancy. We previously showed that the TCE metabolite, S-(1,2-dichlorovinyl)-L-cysteine (DCVC), …

Modeling Functional Connectivity With Learning And Memory In A Mouse Model Of Alzheimer’S Disease, Lindsay Fadel, Elizabeth Hipskind, Steen E Pedersen, Jonathan Romero, Caitlyn Ortiz, Eric Shin, Md Abul Hassan Samee, Robia G Pautler

Faculty, Staff and Students Publications

Introduction: Functional connectivity (FC) is a metric of how different brain regions interact with each other. Although there have been some studies correlating learning and memory with FC, there have not yet been, to date, studies that use machine learning (ML) to explain how FC changes can be used to explain behavior not only in healthy mice, but also in mouse models of Alzheimer's Disease (AD). Here, we investigated changes in FC and their relationship to learning and memory in a mouse model of AD across disease progression.

Methods: We assessed the APP/PS1 mouse model of AD and wild-type controls …

Liberatory Motherhood: A Framework & Praxis For Caring And Scholaring Otherwise In The Neoliberal Academy, Bhavika Sicka

Educational Leadership & Workforce Development Faculty Publications

This article contributes to the emergent literature on motherhood in neoliberal higher education by proposing liberatory motherhood as a theoretical framework and praxis to deconstruct and reconstruct motherhood in the neoliberal academy. The author, an early-career immigrant woman scholar of color, uses feminist autoethnography to critically examine the disempowering, disembodying, and departicularizing effects of neoliberal subjectivation on motheracademics, foregrounding her lived experiences of caregiving in her analysis. She approaches academic motherhood through a prismatic lens of a pluralistic feminist rhetorical analysis that emphasizes interrelationality, tracing her experiences of caring and producing otherwise as a way to signal opportunities to reembody …

Assessing Arbuscular Mycorrhizal Fungal Community Composition And Diversity Across A Land-Use Gradient In Eastern Washington Prairies, Katherine I. Cole

EWU Masters Thesis Collection

Arbuscular mycorrhizal fungi (AMF) are key to healthy, functioning terrestrial ecosystems worldwide. Associating with over 80% of all extant land plants, they play a pivotal role in nutrient cycling, and contribute to overall plant health. Given their significance to terrestrial ecosystems, AMF have a crucial role to play in the successful restoration of Eastern Washington’s native prairies. However, AMF community composition is significantly altered by the commercial agricultural practices used extensively in this region for over 100 years. The Eastern Washington University (EWU) Prairie Restoration Project aims to restore ~120 acres of farmland to native prairie. This research provides insight …

Debt Decisions: The Effects Of Paying For College In The United States With Student Loans, Kim E. Bullington, David J. Thomas, Minghui Hou, William L. Nuckols, Dennis E. Gregory, Joshua L. Howell, Kaycee L. Bills, Amy-Leah Joaquim

Educational Leadership & Workforce Development Faculty Publications

Student loans have increasingly become a burden on individuals. This qualitative analysis explores how structural inequities and financial literacy gaps influence U.S. student loan borrowers’ decisions to finance postsecondary education. The study highlights racial and economic disparities examining participants’ reflections on debt aversion and systemic barriers to equitable access. We asked why participants took out Federal student loans, asked about their decision processes when taking on debt, whether participants took any action to reduce accumulated debt, and if they’d made any discoveries about their decisions to take on student loan debt. After coding and a thematic analysis, five themes and …

Identifying Notch-Regulated Genes Using A Specific Notch Signaling Inhibitor, Hammed Gafar

EWU Masters Thesis Collection

Bone remodeling is a dynamic process that requires a balance between bone resorption by osteoclasts and bone formation by osteoblasts to maintain skeletal integrity. Disruptions to this equilibrium can lead to pathological conditions such as osteoporosis. Osteoclasts originate from macrophages, and their differentiation is regulated by several signaling pathways, notably the notch signaling pathway. Gamma-secretase, an enzyme crucial for initiating notch signaling, can be inhibited by DAPT. However, DAPT also targets non-Notch substrates, complicating the identification of genes specifically regulated by Notch. To address this limitation, this study employed IMR-1A, a selective Notch pathway inhibitor that disrupts the assembly of …

Bridging Prairie Restoration And Regenerative Agriculture With Compost Tea / The Rocket Incident: Post-Fire Soil Microbe Recovery On The Eastern Washington University Restoration Site, April Hersey

EWU Masters Thesis Collection

Inland Pacific Northwest prairie is an endangered ecosystem that has been reduced by conversion to agriculture. There is growing interest in the role of soil microorganisms in prairie ecosystems, which may be crucial for restoration of the plant community through interspecies interactions within the rhizosphere. One method for improving soil microorganism community composition is compost tea: enriched liquid compost extract. Compost tea is used in regenerative agriculture to improve crop yields and protect against pathogens. While research has supported compost tea application in regenerative agriculture, it has not been widely studied in the context of ecosystem restoration. After beginning a …