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Articles 15871 - 15900 of 23220
Full-Text Articles in Entire DC Network
Measuring Perceived Stress Levels And Physiological Variables In Undergraduate College Students: A Quasi-Experimental Study, Sarah J. Milner
Measuring Perceived Stress Levels And Physiological Variables In Undergraduate College Students: A Quasi-Experimental Study, Sarah J. Milner
The Interactive Journal of Global Leadership and Learning
This study aimed to analyze changes in perceived stress and physiological variables in undergraduate students over one semester. A quasi-experimental study was conducted using non-random convenience sampling with 30 undergraduate students enrolled in an Introduction to Stress Management class at a small, regional institution in the upper Midwest in the fall of 2022.
Data collection included the use of the Perceived Stress Scale (i.e., PSS-10) and stress physiological variables (e.g., heart rate, breathing pattern, respiration rate). Participants were surveyed three times using pre-, mid-, and post-treatment assessments but ultimately, participants’ pre- and post-treatment surveys were compared. Both descriptive statistics and …
Unh Research Shows Nerve Cells Move Backward To Reposition Contributing To Brain Evolution, Robbin Ray
Unh Research Shows Nerve Cells Move Backward To Reposition Contributing To Brain Evolution, Robbin Ray
Media Relations
No abstract provided.
Pilliod V. Monsanto Company: Weeding Out Harmful Ingredients, Isabel Rickman
Pilliod V. Monsanto Company: Weeding Out Harmful Ingredients, Isabel Rickman
The Appendix, Journal of Health Care Law & Policy
No abstract provided.
Remaking A Monument: The Troubled Meaning Of Fascist Remains In Contemporary Spain, David Divita
Remaking A Monument: The Troubled Meaning Of Fascist Remains In Contemporary Spain, David Divita
Pomona Faculty Publications and Research
The Valley of Cuelgamuros, until recently known as the Valley of the Fallen, lies about 35 miles outside of Madrid. Inaugurated by the dictator Francisco Franco in 1959, this landmark comprises a vast underground basilica and houses the mostly anonymous remains of 34,000 Spaniards who died during the civil war (1936–1939) and its repressive aftermath. Since the early 2000s, the Valley has been a political lightning rod in a country that has long struggled with how to address the legacies of its authoritarian past. Ongoing debates among politicians, scholars, and journalists have revolved around the possibility of the Valley’s ‘resignification’ …
The Antelope, University Of Nebraska At Kearney
Introducing A Novel Figure Of Merit For Evaluating Stability Of Perovskite Solar Cells: Utilizing Long Short-Term Memory Neural Networks, Zahraa Ismail, Ahmed Alali, Mahmoud Ashraf, Ahmad Muhammad, Sameh O. Abdellatif
Introducing A Novel Figure Of Merit For Evaluating Stability Of Perovskite Solar Cells: Utilizing Long Short-Term Memory Neural Networks, Zahraa Ismail, Ahmed Alali, Mahmoud Ashraf, Ahmad Muhammad, Sameh O. Abdellatif
Electrical Engineering
This study introduces a novel figure of merit for evaluating the stability of perovskite solar cells (PSCs) by employing advanced Long Short-Term Memory (LSTM) neural networks to investigate degradation mechanisms. By harnessing the power of artificial intelligence and data analytics, we analyzed extensive datasets encompassing PSC parameters, experimental results, and environmental conditions, revealing critical insights into the degradation patterns affecting cell performance over time. Our findings indicate that the LSTM model effectively captures and predicts the complex relationships between key design parameters—efficiency, fill factor, and open-circuit voltage—and degradation-induced changes in PSCs. Specifically, we identified three degradation coefficients associated with the …
Floodfriend, Lauren Watson
Floodfriend, Lauren Watson
College of Computing and Digital Media Dissertations
Floodfriend is a two-dimensional puzzle-platformer game with two main characters that are trying to navigate their way out of a cave system. The player controls both the human and the monster of the duo: the hiker responds to direct arrow key input, while the monster can only be persuaded by using the hiker’s flashlight to swim and climb to otherwise inaccessible areas. The game is equal parts a simulation and a toy, encouraging the player to try different methods of interaction to garner the results they want to see. From a design perspective, the game is centered on the push …
Influence Of Inselbergs On Bat Diversity In Afrotropical Ecosystems, Amanda Lee Grunwald
Influence Of Inselbergs On Bat Diversity In Afrotropical Ecosystems, Amanda Lee Grunwald
Dissertations and Theses
Humans are altering biological communities on local to global scales, driving profound changes in biodiversity and ecosystem functioning. However, significant gaps and biases in biodiversity data hinder our understanding of ecosystem interactions, making it challenging to develop effective, data-driven policies to protect remaining biodiversity. Bats, the second most species-rich groups of mammals, have a global distribution, and perform a broad array of vital ecosystem functions. Their diversity peaks in the tropics, where they often constitute more than 50% of mammal species diversity. The Afrotropics host substantial bat diversity, yet have historically been perceived as having lower species richness compared to …
2025 Nebraska Farmland Values And Cash Rental Rates, Jim Jansen
2025 Nebraska Farmland Values And Cash Rental Rates, Jim Jansen
Cornhusker Economics
The market value of agricultural land in Nebraska declined by 2% over the prior year to an average of $3,935 per acre, according to the 2025 Nebraska Farm Real Estate Market Survey. This marks the first decline in the market value of agricultural land in Nebraska since reaching the record non-inflation-adjusted statewide land value in the survey's 46-year history in 2024.
The University of Nebraska-Lincoln’s Department of Agricultural Economics annually surveys land industry professionals across Nebraska, including appraisers, farm and ranch managers, agricultural bankers, and related industry professionals. Results from the survey are divided by land class and summarized by …
Male Sexual Struggles: A Healing Journey, Todd E. Henley
Male Sexual Struggles: A Healing Journey, Todd E. Henley
Doctor of Leadership
My Need Problem or Opportunity (NPO) is that Christian men who commit adultery or engage in pornography are in desperate need of help to heal and be restored by the churches in Parkesburg and Coatesville, Pennsylvania.
One key insight from my research is that churches have continuously struggled to support the men in their congregations as they navigate the challenging journey of healing from issues like pornography and adultery. Through the perspectives of my stakeholders, it became evident that many men often remain unaware of how their past experiences impact their current struggles. Additionally, many carry a deep sense of …
The Lumberjack, March 12, 2025
The Lumberjack, March 12, 2025
Cal Poly Humboldt's Student Newspaper
The student newspaper of Humboldt State University.
Innate Immune Sensing Of Rotavirus By Intestinal Epithelial Cells Leads To Diarrhea, Gaopeng Hou, Juhee Son, Maria Florencia Gomez Castro, Takahiro Kawagishi, Avan Antia, Qiru Zeng, Anna L Deveaux, Hinissan P Kohio, Megan T Baldridge, Siyuan Ding, Et Al.
Innate Immune Sensing Of Rotavirus By Intestinal Epithelial Cells Leads To Diarrhea, Gaopeng Hou, Juhee Son, Maria Florencia Gomez Castro, Takahiro Kawagishi, Avan Antia, Qiru Zeng, Anna L Deveaux, Hinissan P Kohio, Megan T Baldridge, Siyuan Ding, Et Al.
2020-Current year OA Pubs
Diarrhea is the predominant symptom of acute gastroenteritis resulting from enteric infections and a leading cause of death in infants and young children. However, the role of the host response in diarrhea pathogenesis is unclear. Using rotavirus and neonatal mice as a model, we found that oral inoculation of UV-inactivated replication-defective rotavirus consistently induced watery diarrhea by robust activation of cytosolic double-stranded RNA sensing pathways and type III interferon (IFN-λ) secretion. Diarrhea was significantly diminished in mice lacking the IFN-λ receptor. Mechanistically, IFN-λ signaling downregulates the expression of Dra, a chloride and bicarbonate exchanger, which contributes to reduced water absorption. …
Continuous Exposure To An Aversive Mixture: A Means Of Maintaining Aversion In Sheep Averted To Geigeria Ornativa O. Hoffm. (Vermeerbos) - An Assessment, Leendert D. Snyman
Continuous Exposure To An Aversive Mixture: A Means Of Maintaining Aversion In Sheep Averted To Geigeria Ornativa O. Hoffm. (Vermeerbos) - An Assessment, Leendert D. Snyman
Poisonous Plant Research (PPR)
Establishing aversion to Geigeria ornativa only lasts for some time, after which the induced aversion disappears and sheep start eating G. ornativa again. This behavior is aggravated by the social influence of non-averted sheep in mixed grazing situations. Exposing sheep continuously to a so-called “aversive mixture”, containing an aversive substance (lithium chloride) and the sensory characteristics of the plant (hexane extract of G. ornativa) mixed with a tasty meal (maize meal), following an initial aversion treatment, resulted in sustained aversion to G. ornativa in the field. This method proved to be applicable to mixed grazing situations with averted and …
General Biology 2 Laboratory Manual: In Person Edition, Dmitry Y. Brogun, Azure N. Faucette, Farshad Tamari, Kristin Polizzotto
General Biology 2 Laboratory Manual: In Person Edition, Dmitry Y. Brogun, Azure N. Faucette, Farshad Tamari, Kristin Polizzotto
Open Educational Resources
The completed project is an in-person teaching version of the General Biology II OER (Open Educational Resource) lab manual. This lab manual was initially written by the four of us during the pandemic when all courses were online, but it is now has been adapted for in-person lab activities. We also ensured it aligns with UDL (Universal Design for Learning) and accessibility principles. This adaptation aims to make the lab manual suitable for traditional classroom settings while incorporating diverse teaching practices to accommodate diverse learners.
Cost-Effective Active Laser Scanning System For Depth-Aware Deep-Learning-Based Instance Segmentation In Poultry Processing, Pouya Sohrabipour, Chaitanya Kumar Reddy Pallerla, Amirreza Davar, Siavash Mahmoudi, Philip Crandall, Wan Shou, Yu She, Dongyi Wang
Cost-Effective Active Laser Scanning System For Depth-Aware Deep-Learning-Based Instance Segmentation In Poultry Processing, Pouya Sohrabipour, Chaitanya Kumar Reddy Pallerla, Amirreza Davar, Siavash Mahmoudi, Philip Crandall, Wan Shou, Yu She, Dongyi Wang
School of Industrial Engineering Faculty Publications
The poultry industry plays a pivotal role in global agriculture, with poultry serving as a major source of protein and contributing significantly to economic growth. However, the sector faces challenges associated with labor-intensive tasks that are repetitive and physically demanding. Automation has emerged as a critical solution to enhance operational efficiency and improve working conditions. Specifically, robotic manipulation and handling of objects is becoming ubiquitous in factories. However, challenges exist to precisely identify and guide a robot to handle a pile of objects with similar textures and colors. This paper focuses on the development of a vision system for a …
Cost-Effective Active Laser Scanning System For Depth-Aware Deep-Learning-Based Instance Segmentation In Poultry Processing, Pouya Sohrabipour, Chaitanya Palleria, Amirreza Davar, Siavash Mahmoudi, Philip G. Crandall, Wan Shou, Yu She, Dongyi Wang
Cost-Effective Active Laser Scanning System For Depth-Aware Deep-Learning-Based Instance Segmentation In Poultry Processing, Pouya Sohrabipour, Chaitanya Palleria, Amirreza Davar, Siavash Mahmoudi, Philip G. Crandall, Wan Shou, Yu She, Dongyi Wang
Biological and Agricultural Engineering Faculty Publications and Presentations
The poultry industry plays a pivotal role in global agriculture, with poultry serving as a major source of protein and contributing significantly to economic growth. However, the sector faces challenges associated with labor-intensive tasks that are repetitive and physically demanding. Automation has emerged as a critical solution to enhance operational efficiency and improve working conditions. Specifically, robotic manipulation and handling of objects is becoming ubiquitous in factories. However, challenges exist to precisely identify and guide a robot to handle a pile of objects with similar textures and colors. This paper focuses on the development of a vision system for a …
Control Of Clostridioides Difficile Virulence And Physiology By The Flagellin Homeostasis Checkpoint Flic-Fliw-Csra In The Absence Of Motility, Duolong Zhu, Katherine J Wozniak, Firas Midani, Shaohui Wang, Xingmin Sun, Robert A Britton
Control Of Clostridioides Difficile Virulence And Physiology By The Flagellin Homeostasis Checkpoint Flic-Fliw-Csra In The Absence Of Motility, Duolong Zhu, Katherine J Wozniak, Firas Midani, Shaohui Wang, Xingmin Sun, Robert A Britton
Faculty, Staff and Students Publications
Mutations affecting Clostridioides difficile flagellin (FliC) have been shown to be hypervirulent in animal models and display increased toxin production and alterations in central metabolism. The regulation of flagellin levels in bacteria is governed by a tripartite regulatory network involving fliC, fliW, and csrA, which creates a feedback system to regulate flagella production. Through genomic analysis of C. difficile clade 5 strains (non-motile), we identified they have jettisoned many of the genes required for flagellum biosynthesis yet retain the major flagellin gene fliC and regulatory gene fliW. We therefore investigated the roles of fliC, fliW …
A Comparison Of Unconventional Microwave And Ultrasound-Assisted Extraction Methods Used For Flavonoids, Istiqomah Rahmawati, Daffa Hafiziaulhaq Azizi, Jihan Nafila Wibowo, Muhammad Reza, Boy Arief Fachri, Bekti Palupi, Meta Fitri Rizkiana, Helda Wika Amini, Ifan Ramadana, Felix Arie Setiawan
A Comparison Of Unconventional Microwave And Ultrasound-Assisted Extraction Methods Used For Flavonoids, Istiqomah Rahmawati, Daffa Hafiziaulhaq Azizi, Jihan Nafila Wibowo, Muhammad Reza, Boy Arief Fachri, Bekti Palupi, Meta Fitri Rizkiana, Helda Wika Amini, Ifan Ramadana, Felix Arie Setiawan
Makara Journal of Science
Cocoa pods (Theobroma cacao L.) are a rich source of flavonoids, which are natural antioxidants known for their health benefits. This study investigated the use of microwave-assisted extraction (MAE) and ultrasound-assisted ex-traction (UAE) to extract the maximum flavonoids and antioxidants from cocoa pods. MAE and UAE are efficient and sustainable methods for extracting bioactive compounds like flavonoids and antioxidants from cocoa pods, offer-ing faster extraction, reduced solvent use, and better compound preservation compared to conventional methods. These technologies unlock the untapped potential of cocoa pods for applications in food, cosmetics, and pharmaceuti-cals. The effects of extraction time (2–10 min), microwave …
The Expression Profile Of The Rank/Rankl/Opg Pathway In Breast Cancer Stem Cells Isolated From Breast Cancer Cell Lines, Mohamed M. Kamal, Hassnaa H. Elgohary, Sherine Maher Rizk, Nadine H. Maurice
The Expression Profile Of The Rank/Rankl/Opg Pathway In Breast Cancer Stem Cells Isolated From Breast Cancer Cell Lines, Mohamed M. Kamal, Hassnaa H. Elgohary, Sherine Maher Rizk, Nadine H. Maurice
Pharmacy
The RANK/RANKL/OPG signaling pathway plays a crucial role in breast cancer progression and metastasis. However, its expression patterns and potential implications in breast cancer stem cells remain poorly understood. This study aimed to characterize the expression profile of this pathway in breast cancer stem cells isolated from two distinct breast cancer cell lines: MDA‐MB‐231 and MCF‐7. Mammospheres (MS), representing breast cancer stem cells, were generated using agar‐coated 6 well tissue culture plates in suitable mammospheres culture conditions. Flow cytometric analysis showed enrichment of the CD44+/ CD24− subpopulations in the mammospheres cultures, with MDA‐MB‐231 exhibiting a higher percentage compared to MCF‐7. …
Effects Of Space Flight On Inflammasome Activation In The Brain Of Mice, Upal Roy, Roey Hadad, Angel A. Rodriguez, Alen Saju, Deepa Roy, Mario Gil, Robert W. Keane, Ryan T. Scott, Xiao W. Mao, Juan Pablo De Rivero Vaccari
Effects Of Space Flight On Inflammasome Activation In The Brain Of Mice, Upal Roy, Roey Hadad, Angel A. Rodriguez, Alen Saju, Deepa Roy, Mario Gil, Robert W. Keane, Ryan T. Scott, Xiao W. Mao, Juan Pablo De Rivero Vaccari
Health & Biomedical Sciences Faculty Publications
Space flight exposes astronauts to stressors that alter the immune response, rendering them vulnerable to infections and diseases. In this study, we aimed to determine the levels of inflammasome activation in the brains of mice that were housed in the International Space Station (ISS) for 37 days. C57BL/6 mice were launched to the ISS as part of NASA’s Rodent Research 1 Mission on SpaceX-4 CRS-4 Dragon cargo spacecraft from 21 September 2014 to 25 October 2014. Dissected mouse brains from that mission were analyzed by immunoblotting of inflammasome signaling proteins and Electrochemiluminescence Immunoassay (ECLIA) for inflammatory cytokine levels. Our data …
Climate Change Policies And Good Governance In Egypt, Mahmoud Elshourbagy
Climate Change Policies And Good Governance In Egypt, Mahmoud Elshourbagy
Theses and Dissertations
Egypt recognizes the impacts of climate change and is particularly vulnerable because of its high-density population, economic burden and geographical location. In response, Egypt established National Climate Change Council in 2016. In 2022, the council issued “National Climate Change Strategy 2050” to integrate and align the efforts of government bodies to adapt and mitigate climate change risks. One of the strategic goals of “National Climate Change Strategy 2050” is to enhance climate governance in Egypt as a critical tool. This study attempts to evaluate climate change policies in the context of good governance by using a tool developed by World …
Spectrum, Volume 54, Issue 6, Sacred Heart University
Spectrum, Volume 54, Issue 6, Sacred Heart University
Newspapers (Obelisk & Spectrum)
Highlights include: SHU Health Science Class Partners with the CT Mirror - SHU Professor and Student Collaborate on Children’s Book - Senior Week 2025: The Final Show, Off-Campus Food Finds - Celebrating Saint Patrick’s Day in Connecticut - The Stage is Set for TheatreFest Round Three - Short Filmmakers Club Showcases Reel Talent - Men’s Hockey Advances to AHA Semifinals
Comprehensive Review On The Application Of Bio-Immunoinformatics In The Development Of Highly Ef-Fective New Candidate Vaccines Against Tuberculosis, Ahyar Ahmad, Andriansjah Rukmana, Miski A. Khairinisa, Dian A. E. Pitaloka, Rosana Agus, Rusdina B. Ladju, Tarwadi Ahmad, Astutiati Nurhasanah, Carina C. D. Joe, Muhammad N. Massi, Harningsih Karim, Irda Handayani, Siti Roszilawati Binti Ramli
Comprehensive Review On The Application Of Bio-Immunoinformatics In The Development Of Highly Ef-Fective New Candidate Vaccines Against Tuberculosis, Ahyar Ahmad, Andriansjah Rukmana, Miski A. Khairinisa, Dian A. E. Pitaloka, Rosana Agus, Rusdina B. Ladju, Tarwadi Ahmad, Astutiati Nurhasanah, Carina C. D. Joe, Muhammad N. Massi, Harningsih Karim, Irda Handayani, Siti Roszilawati Binti Ramli
Karbala International Journal of Modern Science
Tuberculosis (TB) remains a significant public health challenge worldwide. Currently, Bacillus Calmette-Guerin (BCG) is the only vaccine available for TB prophylaxis. However, the efficacy of the BCG vaccine against adult pulmonary TB is considered inconsistent. This condition encourages researchers to look for more effective options, such as subunit vaccines. This condition requires the development of a more effective subunit vaccine to protect active TB in productive and adult ages. There is an urgent need for more effective vaccines, as the Bacillus Calmette-Guérin (BCG) vaccine currently available has inconsistent efficacy and is only partially effective in adults. Bio-immunoinformatics, an interdisciplinary field …
Articulated Robot Path Planning Based On Hybridization Of Adaptive Dimensionality Algorithm And Grey Wolf Optimizer In Dynamic Environments, Noor Kadhim Ayoob, Ali Hadi Hasan
Articulated Robot Path Planning Based On Hybridization Of Adaptive Dimensionality Algorithm And Grey Wolf Optimizer In Dynamic Environments, Noor Kadhim Ayoob, Ali Hadi Hasan
Karbala International Journal of Modern Science
A new method was developed to plan a path for a robotic articulated vehicle using the Grey Wolf Optimizer (GWO) and Adaptive Dimensionality (AD). Existing studies in robotics path planning ignore the differences between robots in terms of size and flexibility and allocate a single cell to the robot regardless of the mentioned factors. Since the articulated robotic vehicle is longer than obstacles moving in the environment, this study takes into account vehicle size and flexibility in path planning by adapting the number of cells allocated to the robotic vehicle to contain the vehicle parts while performing different movements. Considering …
Ras El-‘Amud, Jerusalem: Faunal Remains From The Intermediate Bronze And Middle Bronze Age Settlement, Nimrod Marom
Ras El-‘Amud, Jerusalem: Faunal Remains From The Intermediate Bronze And Middle Bronze Age Settlement, Nimrod Marom
Qadum: Journal of Excavation Reports from Israel
The small faunal assemblage from Ras el-‘Amud provides insights into the economic strategies of a rural settlement during a period of urban growth. A shift from a self-sufficient goat and pig economy to a specialized sheep-focused one is observed, likely driven by urban demand. Analysis of caprine skeletal remains suggests consumption patterns and potential ritualistic practices. The Middle Bronze IIA village exhibits a decrease in butchery waste, possibly due to changes in spatial organization or waste disposal practices. The assemblage offers valuable insights into the economic and social dynamics of rural settlements during the Middle Bronze Age.
Ras El-‘Amud, Jerusalem: The Groundstone Tool Assemblage, Aaron Greener
Ras El-‘Amud, Jerusalem: The Groundstone Tool Assemblage, Aaron Greener
Qadum: Journal of Excavation Reports from Israel
The excavations at Ras el-‘Amud yielded 71 identified groundstone tools, 55 of which were discovered in the Intermediate Bronze Age (IBA) strata, with the remaining 16 found in the Iron Age and Roman/Byzantine strata and in unclear contexts. These tools mainly comprise hammerstones, likely associated with the production of flint tools at the site, as well as perforated stones, mortars, bowlets, rubbing stones, a grinding stone, a pestle and some other tools. The identified groundstone tools suggest activities related to the processing of cereals, grains and other agricultural produce, as well as possible animal fiber processing and various domestic tasks …
Patterns And Representation In Play-Based Learning: A Systematic Meta-Synthesis Of Empirical Studies In K-13+ Settings, Steph Dean, Julianne A. Wenner
Patterns And Representation In Play-Based Learning: A Systematic Meta-Synthesis Of Empirical Studies In K-13+ Settings, Steph Dean, Julianne A. Wenner
Publications
This systematic review provides a comprehensive meta-synthesis that examines empirical research on the implementation of play-based learning in formal educational settings. The review aims to identify patterns in the enactment of play-based learning, including its representation across academic disciplines, methodologies, grade levels, geographic contexts, and key indicators of play—choice, wonder, and delight. A comprehensive search across nine databases yielded 1,475 studies, of which 87 met stringent inclusion criteria: empirical, K-13+ formal settings, and an intentional learning objective tied to play. We extracted data from each study and used thematic synthesis to analyze patterns across multiple dimensions. Findings indicate that play-based …
Exploring The Impacts Of Food System Education: A Case Study, Constanza Arevalo Msc, Andie Thompkins Ph.D.
Exploring The Impacts Of Food System Education: A Case Study, Constanza Arevalo Msc, Andie Thompkins Ph.D.
Diet
This case study, involving qualitative and quantitative methods, explores the long-term impacts of an education program that trains students in effective communication, advocacy, and leadership skills to combat factory farming. The results from this study will shed light on the effectiveness and value of educational programs for the animal protection movement and for the students who partake in them.
Flapping Dynamics And Wing Flexibility Enhance Odor Detection In Blue Bottle Flies, Naeem Haider, Zhipeng Lou, Chengyu Li
Flapping Dynamics And Wing Flexibility Enhance Odor Detection In Blue Bottle Flies, Naeem Haider, Zhipeng Lou, Chengyu Li
Faculty Scholarship
One of the most ancient and evolutionarily conserved behaviors in the animal kingdom involves utilizing wind-borne odor plumes to track essential elements such as food, mates, and predators. Insects, particularly flies, demonstrate a remarkable proficiency in this behavior, efficiently processing complex odor information encompassing concentrations, direction, and speed through their olfactory system, thereby facilitating effective odor-guided navigation. Recent years have witnessed substantial research explaining the impact of wing flexibility and kinematics on the aerodynamics and flow field physics governing the flight of insects. However, the relationship between the flow field and olfactory functions remains largely unexplored, presenting an attractive frontier …
Necrosis Drives Susceptibility To Mycobacterium Tuberculosis In Polg(D257a) Mutator Mice, C J Mabry, C G Weindel, L W Stranahan, Jordyn J Vanportfliet, J R Davis, E L Martinez, A Phillip West, K L Patrick, R O Watson
Necrosis Drives Susceptibility To Mycobacterium Tuberculosis In Polg(D257a) Mutator Mice, C J Mabry, C G Weindel, L W Stranahan, Jordyn J Vanportfliet, J R Davis, E L Martinez, A Phillip West, K L Patrick, R O Watson
Faculty Research 2025
The genetic and molecular determinants that underlie the heterogeneity of Mycobacterium tuberculosis (Mtb) infection outcomes in humans are poorly understood. Multiple lines of evidence demonstrate that mitochondrial dysfunction can exacerbate mycobacterial disease severity, and mutations in some mitochondrial genes confer susceptibility to mycobacterial infection in humans. Here, we report that mutations in mitochondria DNA (mtDNA) polymerase gamma potentiate susceptibility to Mtb infection in mice. PolgD257A mutator mtDNA mice fail to mount a protective innate immune response at an early infection time point, evidenced by high bacterial burdens, reduced M1 macrophages, and excessive neutrophil infiltration in the lungs. Immunohis tochemistry reveals …