Digital Commons Network^™

The Type Ii Raf Inhibitor Tovorafenib In Relapsed/Refractory Pediatric Low-Grade Glioma: The Phase 2 Firefly-1 Trial, Lindsay B Kilburn, Mohamed S Abdelbaki, Et Al.

2020-Current year OA Pubs

BRAF genomic alterations are the most common oncogenic drivers in pediatric low-grade glioma (pLGG). Arm 1 (n = 77) of the ongoing phase 2 FIREFLY-1 (PNOC026) trial investigated the efficacy of the oral, selective, central nervous system-penetrant, type II RAF inhibitor tovorafenib (420 mg m

Sillyputty: Improved Clustering By Optimizing The Silhouette Width, Polina Bombina, Dwayne Tally, Zachary B. Abrams, Kevin R. Coombes

2020-Current year OA Pubs

Clustering is an important task in biomedical science, and it is widely believed that different data sets are best clustered using different algorithms. When choosing between clustering algorithms on the same data set, reseachers typically rely on global measures of quality, such as the mean silhouette width, and overlook the fine details of clustering. However, the silhouette width actually computes scores that describe how well each individual element is clustered. Inspired by this observation, we developed a novel clustering method, called SillyPutty. Unlike existing methods, SillyPutty uses the silhouette width for individual elements as a tool to optimize the mean …

Inhibition Of Src-3 As A Potential Therapeutic Strategy For Aggressive Mantle Cell Lymphoma, Imani Bijou, Yang Liu, Dong Lu, Jianwei Chen, Shelby Sloan, Lapo Alinari, David M Lonard, Bert W O'Malley, Michael Wang, Jin Wang

Faculty, Staff and Students Publications

Mantle cell lymphoma (MCL) has a poor prognosis and high relapse rates despite current therapies, necessitating novel treatment regimens. Inhibition of SRC-3 show effectiveness in vivo and in vitro in other B cell lymphomas. Additionally, previous studies have shown that SRC-3 is highly expressed in the lymph nodes of B cell non-Hodgkin's lymphoma patients, suggesting SRC-3 may play a role in the progression of B cell lymphoma. This study aimed to investigate novel SRC-3 inhibitors, SI-10 and SI-12, in mantle cell lymphoma. The cytotoxic effects of SI-10 and SI-12 were evaluated in vitro and demonstrated dose-dependent cytotoxicity in a panel …

Diagnostic Value Of Soluble Urokinase-Type Plasminogen Activator Receptor In Patients With Acute Coronary Syndrome: A Systematic Review And Meta-Analysis, Michal Pruc, Iwona Jannasz, Damian Swieczkowski, Grzegorz Procyk, Aleksandra Gasecka, Zubaid Rafique, Francesco Chirico, Nicola Luigi Bragazzi, Milosz J Jaguszewski, Jaroslaw Wysocki, Lukasz Szarpak

Faculty, Staff and Students Publications

BACKGROUND: In contemporary clinical practice, there is an increasing need for new clinically relevant biomarkers potentially optimizing management strategies in patients with suspected acute coronary syndrome (ACS). This study aimed to determine the diagnostic utility of soluble urokinase-type plasminogen activator receptor (suPAR) levels in individuals with suspected ACS.

METHODS: A literature search was performed in Web of Science, PubMed, Scopus, and the Cochrane Central Register of Controlled Trials databases, for studies comparing suPAR levels among patients with and without ACS groups. The methodological quality of the included papers was assessed using the Newcastle-Ottawa Scale (NOS). A fixed-effects model was used …

Smooth Muscle Contractile Responses To Bile Acids In Mouse Ileum Require Tgr5 But Not Asbt, Diana S Chang, Krishnakant G Soni, Geoffrey A Preidis

Faculty, Staff and Students Publications

BACKGROUND: Many disorders of gut-brain interaction (DGBIs) are more prevalent in women than men and feature alterations in gastrointestinal motility and bile acid homeostasis. Mechanisms by which bile acids regulate gastrointestinal motility are poorly characterized. We recently validated an adapted tissue bath technique using everted mouse ileum, which revealed differential contractile responses to ursodeoxycholic acid (UDCA) and deoxycholic acid (DCA). Here, we aimed to determine whether these responses are dependent on host sex, the plasma membrane bile acid receptor TGR5, or the apical sodium-dependent bile acid transporter ASBT.

METHODS: Ileal segments from male and female mice were everted and suspended …

The Impact Of Race On Pancreatic Cancer Treatment And Survival In The Nationwide Veterans Affairs Healthcare System, Natalia Khalaf, Ann Xu, Theresa Nguyen Wenker, Jennifer R Kramer, Yan Liu, Hardeep Singh, Hashem B El-Serag, Fasiha Kanwal

Faculty, Staff and Students Publications

OBJECTIVES: Among patients with pancreatic cancer, studies show racial disparities at multiple steps of the cancer care pathway. Access to healthcare is a frequently cited cause of these disparities. It remains unclear if racial disparities exist in an integrated, equal access public system such as the Veterans Affairs healthcare system.

METHODS: We identified all patients diagnosed with pancreatic adenocarcinoma in the national Veterans Affairs Central Cancer Registry from January 2010 to December 2018. We examined the independent association between race and 3 endpoints: stage at diagnosis, receipt of treatment, and survival while adjusting for sociodemographic factors and medical comorbidities.

RESULTS: …

Integrating Christian Beliefs And Scientific Psychology: Honoring John Carter, Rodger K. Bufford

Faculty Publications - Doctor of Psychology (PsyD) Program

John Carter had a profound role in shaping the thinking of generations of students and scholars in our understanding of the relationship of Christian beliefs and modern psychol- ogy. That Carter and Narramore’s (1979) volume remains in print and in use after more than four decades is a testimony to that influence. Here, I briefly explore the ways of knowing in general and elaborate on scientific knowing as a unique and powerful approach to appre- hending what our senses and instruments reveal. Scientific knowing is premised on several essential assumptions, employs a time-tested array of strategies to systematically gath- er …

The Role Played By Non-Formal Education In The Lives Of Iraqi-British Citizens: A Study Of Three Iraqi-British Cultural Institutions And How Non-Formal Education Empowers Individuals And Communities, Nadeem Al-Abdalla

School of Law, Humanities and Social Sciences Theses

This thesis highlights the role played by non-formal education in the lives of Iraqi community members who participate in the activities offered by London’s Iraqi-British cultural centres. The key aims of this thesis were; to research, document and analyse London’s Iraqi community and their experiences of non-formal learning within Iraqi cultural centres; and to establish the extent to which they found their participation in this non-formal learning empowering, both at an individual and community level. Many studies have been conducted into the lives of newly arrived communities. Generally, these studies tend to focus on their early years in the UK …

Exposure To Metal Accumulation In Chicken Giblets And Human Health Risk In Lokoja, Nigeria, Jude Ehwevwerhere Emurotu, Lami Habib, Ephraim Musa Dallatu, Adamu Shuaibu Onakpa, Aloysius Akaangee Pam

Applied Environmental Research

When consumed, the toxic heavy metals in chicken giblets pose a grave risk to humans. An inductively coupled plasma optical emission spectrometer (ICP-OES) was used to measure the concentrations of metals (Al, Cd, Co, Cr, Ni, Mn, and Pb) in the liver, gizzard, and kidney of chickens from the Lokoja poultry market. Except for lead, which slightly exceeded the FAO/WHO limit of 0.1 mg kg-1, the metal concentrations found were all within the threshold limit. The estimated daily intake (EDI), hazard quotient (HQ), hazard index (HI), and cancer risk (CR) were used to assess the risks that humans would face …

Unveiling Long-Term Impacts Of Forest Cover Changes And Carbon Storage Assessment In Nan Province, Thailand, Jiratiwan Kruasilp, Sura Pattanakiat, Siam Lawawirojwong, Thamarat Phutthai, Poonperm Vardhanabindu, Warinthorn Kiadtikornthaweeyot Evans, Anne Jacquine

Applied Environmental Research

Forests serve as carbon sink by storing and sequestrating carbon dioxide from the atmosphere. Forest cover loss adversely affects communities by increasing risks to the shortage of carbon storage. This study provides a comprehensive understanding of forest fragmentation and assessment of forest cover changes impacts on carbon storage over Nan province during 1990 to 2019. Spatial and temporal patterns were explored using landscape metrics analysis. Forest areas declined 10.69% over 30 years using random forest classifier based on Google Earth Engine platform, with an overall accuracy of 97.10%. The highest rate of forest cover changes was 5.27% (2010 to 2014), …

Minimization The Chemical Oxygen Demand (Cod) Content In Tannery Wastewater Using Activated Carbon From Spent Tea Leaves, Kanish Fatama, Nasifa Akter, Badhan Saha, Nur -E- Alam Md., Shimul Chakma

Applied Environmental Research

Water pollution from tannery wastewater is a serious environmental issue. This study aimed to investigate the potential of spent tea leaves as activated carbon (AC) to minimize chemical oxygen demand (COD) of tannery effluent. To determine the best conditions for minimizing COD, a batch adsorption process was conducted, involving various factors such as different adsorbent dosages (ranging from 1 to 11 gm L-1), contact time (30 to150 min), and pH values (ranging from 3 to 11). The characterization was done by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM). The outcomes of this study demonstrated that using 1.5 M …

Adsorption Behavior And Mechanism Of Brilliant Green Dye Onto Acid-Modified Mesoporous Volcanic Pumice, Suchada Sawasdee, Prachart Watcharabundit

Applied Environmental Research

Water pollution is a major problem in the environment. The important one is the dying effluent from the textile industry. Dye effluent has affected human health and aquatic life. Recently, natural adsorbents have been most commonly used for dye treatment wastewater. This study explores the adsorption mechanism of brilliant green onto HCl-modified pumice. It involves a comparative analysis of the raw and HCl-modified pumice's characterization and adsorption properties. The adsorption experiments were carried out by considering several parameters, such as pH, contact time, initial dye concentrations, and temperatures. The research indicated that the raw and acid-treated pumice possess Si-OH and …

Enzyme Activity And Biodegradation Potentials Of Serratia Marcescens Cell-Free Extract On Polyethylene Terephthalate Sheet, Ogunjemite Oluwadamilola E, Folasade M. Olajuyigbe

Applied Environmental Research

Microbial strains with potential hydrolytic enzymes are excellent hosts for the biodegradation of polyethylene terephthalate (PET). However, knowledge of the best working conditions for enzymatic actions is essential to suggest the most suitable condition for the bio-transformation process. In this research, PETase and lipase produced by Serratia marcescens under PET exposure were screened and their physicochemical properties were evaluated. Furthermore, the biodegradation efficiency of the cell-free extract produced from S. marcescens on a PET sheet was studied via scanning electron microscope (SEM) and Fourier transform infrared spectrophotometer (FTIR). The result showed maximum production of PETase activity (8.80 U mL-1) and …

Effective Microorganisms (Em) As Bioremediation Agent Of Profenofos Pesticide Residue In Vegetable Farm Soil From Benguet Province, Philippines, Maylowen P. Lumayna, Paulina A. Bawingan

Applied Environmental Research

Effective microorganisms (EM) have shown remarkable adaptability and have been used in agriculture and environmental management. This study intended to provide insights into the effectiveness of EM technology in rehabilitating soils contaminated with pesticides. Specifically, the study aimed to determine the efficiency of EM in breaking down the profenofos component of Selecron® 500 EC pesticide in a controlled condition. Results show that the initial profenofos (17.2 mg kg-1) degraded in the control and experimental groups by 94.19% and 96.45%, respectively, over a 21-day laboratory experiment. EM-treated soil samples showed a significant difference from untreated samples, as revealed in the Kruskal-Wallis …

Microplastic Contamination In Table Salt: A Study Of Consumer Behavior In Cox's Bazar, Bangladesh, Sk Abid Md Saad, Faijunnesa Rashid, Maisha Miskat, Mukul Chandra Sarker, Arman Khan, Mustafizur Rahman

Applied Environmental Research

Plastic particles resulting from the breakdown of bigger plastics may be introduced directly as micro- and nano-sized particles polluting the marine ecosystem, including ocean-extracted table salt. This study investigated the presence of microplastic (MP)-like particles in table salt from Bangladesh, focusing on both locally refined and commercially branded varieties. Local salt samples from Sadar and Moheshkhali Sub-district of Cox’s Bazar contained an average of 23.4 and 12 particles kg-1, respectively. In contrast, no particles were detected in commercial brands, likely due to their complex refining processes. The study also explored factors influencing local salt consumption, finding it influenced by the …

Global Perspectives On Healthcare Waste Management And Bibliographic Mapping During The Crises Of Covid-19, Vasudeo Zambare, Bhushan Bhusare, Lew Christopher, Mohanadoss Ponraj, Nabin Narayan Munankarmi, Mohd Fadhil Md. Din

Applied Environmental Research

The lack of proper waste management of health care facilities can have a significant negative health impact on the global population. This study provides a critical view on the current state of the existing healthcare waste management practices used in healthcare facilities around the world during the Coronavirus disease 2019 (COVID-19) pandemic outbreak. The increasing amount of COVID-related healthcare waste was analyzed and classified to provide justification for the urgent need to develop waste management standards that ensure safe containment and diversion of hazardous and non-hazardous wastes through recycling, reuse and repurposing. The waste handling, treatment, and disposal management of …

Assessment Of Total Petroleum Carbon And Heavy Metals Of Some Selected Plants Grown In Crude Oil Polluted Soils Using Gc-Fid, Pius O. Adah, Ndem E. Edu, Reagan B. Agbor, Henry B. Kogbara

Applied Environmental Research

This study was focused on assessing the phytoremediation potentials of some selected plant species on crude oil contaminated soils. Five plant species were cultivated in soils polluted with crude oil at 10, 50, 100, 150 and 200 mg kg-1. The results revealed that there were significant reduction in THC contents of soil ameliorated with the various plants species compared to the control group increases with increasing concentration of crude oil pollutant in soil. THC was 62.24 mg kg-1, 1,120 mg kg-1, 208.67 mg kg-1, 257.87 mg kg-1 and 2,762.79 mg kg-1 for R. simplex, P. balfouriana, K. pinnata …

Food Waste From Catering Services In Thai Hotels: A Composition Analysis And Practical Guidelines For Effective Utilization, Patranit Srijuntrapun, Patinya Sukwong

Applied Environmental Research

Food waste from the hotel sector is a significant contributor to the overall volume of food waste, particularly in restaurants and food services through various processes. However, precise data on food waste generated by the hotel industry is lacking. This study aims to quantify and characterize the food waste generated during the preparation and serving processes in hotel catering, with the goal of finding efficient solutions to reduce food waste that are appropriate for the hotel and catering sectors. The study presents novel findings that among the different types of catering, dinner-style buffets (DB) generate the highest amount of food …

Determinants Of Physicochemical Composition Of Palm Oil Mill Effluent - Implications On Environment And Bio-Digester Treatment Design, E.B. Tambe, A.U. Okonkwo, I.E. Mbuka-Nwosu, C.O. Cookey, E. Agbe, S.C. Onwusa, I.N. Ekpe, U.F. Evuen, J. Okpoghono

Applied Environmental Research

Growing need for renewable energy and addressing challenges associated with indiscriminate wastes disposal has necessitated harnessing wastes to wealth. The study investigated parameters determining the physicochemical composition of palm oil mill effluent (POME) generated at Agricultural Development Authority Palm (ADAPALM) and palm oil mills in its catchment communities, located at Ohaji/ Egbema Local Government Area of Imo State, Nigeria and their implications on environmental health and treatment approaches required. Survey research design was used. From a randomly sampled small-scaled mill in each community, and for the lone medium and large-scaled mills, four homogenous samples of POME were collected from sampled …

Typhoon Damage Assessment Of Natural And Planted Mangroves In Bais Bay, Negros Oriental, Philippines, Myron Gavriel F. Baring, Pablina L. Cadiz

Applied Environmental Research

Category 5 Typhoon Odette, internationally known as Rai, devastated the Central Philippines in December 2021, causing immense damage to the country’s infrastructure, agriculture, and ecosystems. Coastal vegetative ecosystems, which include mangroves, are exposed to strong wind and wave energy during extreme weather events. Considering their importance in providing a myriad of ecosystem services to coastal communities, the post-typhoon assessment of mangroves will serve as a guide to future rehabilitation efforts.  On August 13 and 14, 2024, 8 months after Typhoon Odette, the community structure characteristics (species composition, stem density, basal area, and regeneration potential) and damage severity of planted and …

Addressing The Consumer Food Waste Crisis: A Decade Of Psychological Interventions, Pei ๋Jun Woo, Yohanna Veshanya, Lai Ti Gew

Applied Environmental Research

Food waste has become an increasing global crisis, especially in the last few years. Researchers and policymakers have attempted to work towards meeting the Sustainable Development Goals (SDGs) proposed by the United Nations. While much research has been conducted, we are still far from achieving the SDG by 2030. Many interventions have been studied, but each outcome varies. Thus, this review summarizes 30 psychological-based interventions between 2010 to 2022, retrieved via the Scopus database. It is found that each method of intervention works differently based on each type of food waste. Institutional food waste appears to be the most common …

Removal Of Chromium From Aqueous System Using Nanocellulose Prepared From Defoliated Terminalia Catappa Leaves, Lopamudra Digal, Jayashree Mohanty

Applied Environmental Research

Nanocellulose is considered as a sustainable and renewable nanomaterial due to its abundant availability, biodegradability and biocompatibility characteristics. The study demonstrates the synthesis of nanocellulose from defoliated leaves of the Tropical Almond (Terminalia catappa) plant, abundantly available residual plant biomass and its subsequent use in eliminating Cr(VI) from the water system. The study demonstrates the preparation of nanocellulose by chemical means, employing NaOH and H2SO4 as chemical agents. Characterization techniques like FTIR, SEM, and XRD were used to study the chemical composition and morphology of the resulting nanocellulose. The characterization studies confirm the formation of nanocellulose from the plant biomass. …

Fenton And Photo-Fenton For Treatment Of Red Water Containing Trinitrotoluene: A Comparative Study, Nguyen Trung Dung, Le Thanh Dat, Hoang Thi Tue Minh, Nguyen Phuong Thao, Nguyen Thi Thuy, Nguyen Thi Cam Tien, Nguyen Nhat Huy

Applied Environmental Research

Wastewater originating from the production of TNT explosives (red water) contains special hazardous substances that must be treated before discharging into the water environment. In this study, we applied both conventional Fenton and photo-Fenton (UV-Fenton) processes to treat COD and color in red water. In the Fenton process, the COD and color removal efficiencies were 75.5 and 92.4%, respectively, under optimal operating conditions of pH = 3, [Fe2+] = 0.3 g L-1, H2O2/Fe(Ⅱ) ratio = 10:1, reaction time = 120 min. In the UV-Fenton process, the efficiencies were 76.1 and 94.2%, respectively, under optimal operating conditions of pH = 3, …

Theoretical Approaches To Food Waste Behaviours: An Overview, Dane Chia, Chin Choo Yap, Mohamed Kheireddine Aroua, Lai Ti Gew

Applied Environmental Research

Food waste around the world has emerged as a pressing issue that calls for attention and intervention. Analysing the food waste problem without understanding wasteful behaviour antecedents may oversimplify this multi-faceted issue, which will then compromise the development of interventions. This paper provides an overview of the theoretical approaches to food waste behaviour antecedents, followed by evidence-based interventions, using the Theory of Planned Behaviour (TPB), the Norm Activation Model (NAM), and the Social Practice Theory (SPT). These theories encompass the psychological, behavioural, societal, material, and structural factors that influence food waste practices. Our aim is to provide a holistic understanding …

Unraveling The Effects Of Arbuscular Mycorrhizal Fungi On Plant Growth, Nutrient Content, And Heavy Metal Accumulation In The Contaminated Soil: A Meta-Analysis, Bela Putra, Anuraga Jayanegara, Irwan Susanto

Applied Environmental Research

This study evaluated the effects of arbuscular mycorrhizal fungi (AMF) on plant growth, nutrient uptake, and heavy metal accumulation on polluted land using a meta-analysis approach. Data from 33 relevant studies were selected based on inclusion criteria, specifically articles in English, observational research, and investigating the role of AMF in plant growth and productivity on polluted land. The results showed that plants inoculated with AMF experienced significant accumulation of heavy metals in roots, such as Pb (p<0.01), Ni (p<0.01), Cr (p<0.01), Mn (p<0.05), Fe (p<0.05), and As (p<0.05). The AMF significantly reduced the accumulation of heavy metals such as Cr, Ni, Fe, and Cu on the upper part of fodder forage (p<0.01). Forage growth was also enhanced due to AMF. The AMF greatly increased the fresh weight, length, and phosphorus (P) content of fodder forage roots (p<0.01). It also increased the plant's biomass, fresh weight, dry weight, height, nitrogen (N), phosphorus (P), and potassium (K) contents (p<0.01). In conclusion, AMF is important in increasing plant growth, nutrient uptake and reducing heavy metal accumulation in forage on polluted land.

Analyzing Climate Change Status Through Evaluating Trend Of Temperature And Rainfall And Predicting Future Climate Change Status At Lake Tana Basin, Tesfaye Bayu Zeleke, Tri Retnaningsih Soeprobowati, Solomon Adissu, Meseret Abatechanie

Applied Environmental Research

The trends of temperature and rainfall are critical indicators of climate change within a certain area. However, the existence of climate change is not locally understood in most parts of the world. This research aims to analyze the trend of temperature and rainfall in the Lake Tana Sub-basin as a means to understand the current and future status of climate change. The trends of temperature and rainfall were analyzed using the modified Mann-Kendall trend test, while the autoregressive integrated moving average model (ARIMA) was used to predict future temperature and rainfall. The findings reveal that monthly temperatures show a significant …

Ball-Milled Biochar From Waste Bamboo Chopsticks: A Potential Adsorbent For Methylene Blue Removal, Nguyen Trung Hiep, Hoang Thi Thuy Hoa, Thai Phuong Vu

Applied Environmental Research

Waste bamboo chopsticks encounter limited recycling. Several treatment procedures necessitate sophisticated technologies and intricate processes that need more practicality for local-scale production, leading to substantial challenges in effectively repurposing waste from disposable bamboo chopsticks. In this research, waste bamboo chopsticks were used as raw materials for the production of ball-milled biochar (BM-WBCB). FTIR, SEM, and BET were among the numerous analytical methods used to describe the BM-WBCB. The outcomes showed that the surface was made up of hollow, porous structures. In addition, the pHpzc value of the biochar was 8.7, and it had a BET-specific surface area (273.11 m2 g-1). …

Groundwater Recharge And Surface Runoff Modeling Response To Land Use And Land Cover Dynamics In A Mae Wong Watershed Of Thailand, Banchongsak Faksomboon, Pranee Lertkaeo, Bunchongsri Phunlao

Applied Environmental Research

This study aims to develop efficient management strategies and assess the spatiotemporal dynamics of land use and land cover (LULC) on surface runoff modeling response dynamics for the long-term sustainability of watersheds. The soil and water assessment tool (SWAT) model was used to evaluate the LULC dynamics on GRSR in the Mae Wong Watershed (MWW) of Thailand. Using Landsat images, three different LULC maps (2011, 2021, and 2031) were created using the cellular automata markov chain (CA-Markov) model, and TerrSet 2020 geospatial monitoring and modeling software. In the overall MWW, the forestland has undergone deforestation and decreased by 2.10% of …

Exploring 3d Genome Interaction And Epigenetic Regulation Via Swi/Snf Complex And Deep Learning Models, Ruoyun Wang

Dartmouth College Ph.D Dissertations

The three-dimensional organization of the genome is fundamental in regulating gene expression and maintaining cellular function. This organization's complexities, influenced by epigenetic marks and chromatin remodeling complexes, are crucial for understanding genomic regulation. Among these, the SWI/SNF complexes are key, facilitating chromatin accessibility and regulating gene activity across cell types. The first part of my dissertation focuses on SWI/SNF complexes, exploring their role in chromatin remodeling and their impact on 3D genome architecture. Utilizing next-generation sequencing (NGS) techniques, this section investigates the interplay between these complexes and chromatin structure. During my research on the SWI/SNF complex, I was intrigued by …

Feeding The Good Fire: Paths To Facilitate Native-Led Fire Management On Federal Lands, Kevin Burdet

Seattle University Law Review

In 2003, nearly twenty Native American reservations were devastated by wildfires that originated on adjacent federal lands. The San Pasqual Reservation’s entire 1,400 acres were burned along with over a third of its homes, and seventy-five percent of the Rincon Reservation was burned, taking twenty homes with it. These devastating fires, along with others in 2002, brought about the Tribal Forest Protection Act of 2004 (TFPA), which offered hope for Tribes to propose projects on bordering or adjacent federal lands and protect reservation lands in the process. Unfortunately, twenty years later, the TFPA has had a marginal effect in enabling …