Digital Commons Network^™

Identifying New Cancer Genes Based On The Integration Of Annotated Gene Sets Via Hypergraph Neural Networks, Chao Deng, Hong-Dong Li, Li-Shen Zhang, Yiwei Liu, Yaohang Li, Jianxin Wang

Computer Science Faculty Publications

Motivation

Identifying cancer genes remains a significant challenge in cancer genomics research. Annotated gene sets encode functional associations among multiple genes, and cancer genes have been shown to cluster in hallmark signaling pathways and biological processes. The knowledge of annotated gene sets is critical for discovering cancer genes but remains to be fully exploited.

Results

Here, we present the DIsease-Specific Hypergraph neural network (DISHyper), a hypergraph-based computational method that integrates the knowledge from multiple types of annotated gene sets to predict cancer genes. First, our benchmark results demonstrate that DISHyper outperforms the existing state-of-the-art methods and highlight the advantages of …

Hite: A Fast And Accurate Dynamic Boundary Adjustment Approach For Full-Length Transposable Element Detection And Annotation, Kang Hu, Peng Ning, Minghua Xu, You Zou, Jianye Chang, Xin Gao, Yaohang Li, Jue Ruan, Bin Hu, Jianxin Wang

Computer Science Faculty Publications

Recent advancements in genome assembly have greatly improved the prospects for comprehensive annotation of Transposable Elements (TEs). However, existing methods for TE annotation using genome assemblies suffer from limited accuracy and robustness, requiring extensive manual editing. In addition, the currently available gold-standard TE databases are not comprehensive, even for extensively studied species, highlighting the critical need for an automated TE detection method to supplement existing repositories. In this study, we introduce HiTE, a fast and accurate dynamic boundary adjustment approach designed to detect full-length TEs. The experimental results demonstrate that HiTE outperforms RepeatModeler2, the state-of-the-art tool, across various species. Furthermore, …

The Combined Focal Loss And Dice Loss Function Improves The Segmentation Of Beta-Sheets In Medium-Resolution Cryo-Electron-Microscopy Density Maps, Yongcheng Mu, Thu Nguyen, Bryan Hawickhorst, Willy Wriggers, Jiangwen Sun, Jing He

Computer Science Faculty Publications

Although multiple neural networks have been proposed for detecting secondary structures from medium-resolution (5–10 Å) cryo-electron microscopy (cryo-EM) maps, the loss functions used in the existing deep learning networks are primarily based on cross-entropy loss, which is known to be sensitive to class imbalances. To monitor and tune the performance of various loss functions for the secondary structure detection problem, we investigated five loss functions: cross-entropy, Focal loss, Dice loss, and two combined loss functions. Using a U-Net architecture in our DeepSSETracer method and a dataset composed of 1,355 box-cropped atomic-structure/density-map pairs, we found that a newly designed loss function …

Flexible Fitting Of Alphafold2-Predicted Models To Cryo-Em Density Maps Using Elastic Network Models: A Methodological Affirmation, Maytha Alshammari, Jing He, Willy Wriggers

Computer Science Faculty Publications

Motivation: This study investigates the flexible refinement of AlphaFold2 models against corresponding cryo-electron microscopy (cryo-EM) maps using normal modes derived from elastic network models (ENMs) as basis functions for displacement. AlphaFold2 generally predicts highly accurate structures, but 18 of the 137 models of isolated chains exhibit a TM-score below 0.80. We achieved a significant improvement in four of these deviating structures and used them to systematically optimize the parameters of the ENM motion model.

Results: We successfully refined four AlphaFold2 models with notable discrepancies: lipid-preserved respiratory supercomplex (TM-score increased from 0.52 to 0.69), flagellar L-ring protein (TM-score increased from 0.53 …

Sccad: Cluster Decomposition-Based Anomaly Detection For Rare Cell Identification In Single-Cell Expression Data, Yunpei Xu, Shaokai Wang, Qilong Feng, Jiazhi Xia, Yaohang Li, Hong-Dong Li, Jianxin Wang

Computer Science Faculty Publications

Single-cell RNA sequencing (scRNA-seq) technologies have become essential tools for characterizing cellular landscapes within complex tissues. Large-scale single-cell transcriptomics holds great potential for identifying rare cell types critical to the pathogenesis of diseases and biological processes. Existing methods for identifying rare cell types often rely on one-time clustering using partial or global gene expression. However, these rare cell types may be overlooked during the clustering phase, posing challenges for their accurate identification. In this paper, we propose a Cluster decomposition-based Anomaly Detection method (scCAD), which iteratively decomposes clusters based on the most differential signals in each cluster to effectively separate …

Retrogressive Document Manipulation Of Us Federal Environmental Websites, Lesley Frew, Michael L. Nelson, Michele C. Weigle

Computer Science Faculty Publications

Changes made to webpages can affect their retrievability. Often this is done with the intention of increasing the page's search engine ranking to improve overall access to information on the page. The Environmental Data and Governance Initiative (EDGI) created a dataset that describes changes on US federal environmental webpages between 2016 and 2020. EDGI noted that many environmental terms were deleted from the pages, but without user data, claims that page retrievability and public information access were lowered are only anecdotal. The Open Resource for Click Analysis in Search (ORCAS) dataset was created during the same time frame, from 2017 …

Identifying Patterns For Neurological Disabilities By Integrating Discrete Wavelet Transform And Visualization, Soo Yeon Ji, Sampath Jayarathna, Anne M. Perrotti, Katrina Kardiasmenos, Dong Hyun Jeong

Computer Science Faculty Publications

Neurological disabilities cause diverse health and mental challenges, impacting quality of life and imposing financial burdens on both the individuals diagnosed with these conditions and their caregivers. Abnormal brain activity, stemming from malfunctions in the human nervous system, characterizes neurological disorders. Therefore, the early identification of these abnormalities is crucial for devising suitable treatments and interventions aimed at promoting and sustaining quality of life. Electroencephalogram (EEG), a non-invasive method for monitoring brain activity, is frequently employed to detect abnormal brain activity in neurological and mental disorders. This study introduces an approach that extends the understanding and identification of neurological disabilities …

Triphlapan: Predicting Hla Molecules Binding Peptides Based On Triple Coding Matrix And Transfer Learning, Meng Wang, Chuqi Lei, Jianxin Wang, Yaohang Li, Min Li

Computer Science Faculty Publications

Human leukocyte antigen (HLA) recognizes foreign threats and triggers immune responses by presenting peptides to T cells. Computationally modeling the binding patterns between peptide and HLA is very important for the development of tumor vaccines. However, it is still a big challenge to accurately predict HLA molecules binding peptides. In this paper, we develop a new model TripHLApan for predicting HLA molecules binding peptides by integrating triple coding matrix, BiGRU + Attention models, and transfer learning strategy. We have found the main interaction site regions between HLA molecules and peptides, as well as the correlation between HLA encoding and binding …

Ecu-Mss-2 Dataset, A New Multi-Species Seagrass, Md K. Noman, Syed M. S. Islam, Seyed M. Jalali, Jumana Abu-Khalaf, Paul Lavery

Research Datasets

The "ECU-MSS-2' dataset contains four different habitats, 'Amphibolis' spp (hereafter 'Amphibolis"), 'Halophila' spp (hereafter 'Halophila'), 'Posidonia' spp (hereafter 'Posidonia') and 'Background'. We compiled this image dataset from different sources. The 'Halophila' images were collected by the Centre for Marine Ecosystems Research, Edith Cowan University, Western Australia.

The 'Amphibolis' 'Background' and 'Posidonia' images were collected by the Department of Biodiversity, Conservation and Attractions (DBCA), Australia. The 'Amphibolis' class includes the seagrass species Amphibolis griffithii and Amphibolis Antarctica. The 'Halophila' class includes Halophila ovalis, while the 'Posidonia' class includes Posidonia sinuosa, Posidonia coriacea, and Posidonia Australis. The 'Background' class includes coral, sand, …

Investigating Inland Waterway Transport Through A Sustainable Development Perspective: A Case Study Of Magdalena River (Colombia), Natalia Calderón-Rivera

World Maritime University Ph.D. Dissertations

The development of transport systems significantly influences economies, growth, and welfare of populations. However, these systems can have negative consequences, such as water and air pollution, traffic congestion, noise, and biodiversity loss. To address these challenges, it is essential to implement strategies that mitigate negative effects while improving the environmental, social, and economic conditions of the population. In this regard, inland water transport (IWT) is globally recognized as a more sustainable transport alternative due to its high cargo load capacities and comparatively lower environmental and social impacts. In Colombia, the Magdalena River – the country’s most important river basin – …

In Vivo Measurement Of Nadh Fluorescence Lifetime In Skeletal Muscle Via Fiber-Coupled Time-Correlated Single Photon Counting, Kathryn M. Priest, Jacob V. Schluns, Nathania Nischal, Colton L. Gattis, Jeffery C. Wolchok, Timothy J. Muldoon

Biomedical Engineering Faculty Publications and Presentations

Nicotinamide adenine dinucleotide (NADH) is a cofactor that serves to shuttle electrons during metabolic processes such as glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation (OXPHOS). NADH is autofluorescent, and its fluorescence lifetime can be used to infer metabolic dynamics in living cells. Fiber-coupled time-correlated single photon counting (TCSPC) equipped with an implantable needle probe can be used to measure NADH lifetime in vivo, enabling investigation of changing metabolic demand during muscle contraction or tissue regeneration. This study illustrates a proof of concept for point-based, minimally-invasive NADH fluorescence lifetime measurement in vivo. Volumetric muscle loss (VML) injuries were …

Loss Of Β-Cell Identity And Dedifferentiation, Not An Irreversible Process?, Sumit Patel, Maria S Remedi

2020-Current year OA Pubs

Type 2 diabetes (T2D) is a polygenic metabolic disorder characterized by insulin resistance in peripheral tissues and impaired insulin secretion by the pancreas. While the decline in insulin production and secretion was previously attributed to apoptosis of insulin-producing β-cells, recent studies indicate that β-cell apoptosis rates are relatively low in diabetes. Instead, β-cells primarily undergo dedifferentiation, a process where they lose their specialized identity and transition into non-functional endocrine progenitor-like cells, ultimately leading to β-cell failure. The underlying mechanisms driving β-cell dedifferentiation remain elusive due to the intricate interplay of genetic factors and cellular stress. Understanding these mechanisms holds the …

Cep120 Is Essential For Kidney Stromal Progenitor Cell Growth And Differentiation, Ewa Langner, Tao Cheng, Eirini Kefaloyianni, Charles Gluck, Baolin Wang, Moe R Mahjoub

2020-Current year OA Pubs

Mutations in genes that disrupt centrosome structure or function can cause congenital kidney developmental defects and lead to fibrocystic pathologies. Yet, it is unclear how defective centrosome biogenesis impacts renal progenitor cell physiology. Here, we examined the consequences of impaired centrosome duplication on kidney stromal progenitor cell growth, differentiation, and fate. Conditional deletion of the ciliopathy gene Cep120, which is essential for centrosome duplication, in the stromal mesenchyme resulted in reduced abundance of interstitial lineages including pericytes, fibroblasts and mesangial cells. These phenotypes were caused by a combination of delayed mitosis, activation of the mitotic surveillance pathway leading to apoptosis, …

Expression Of Growth Differentiation Factor 15 (Gdf-15) In Placental Tissue Of Full- Term Pregnant Women With Hypertensive Disorders In A Western Australian Population, Elsa H. L. Chew

Theses: Doctorates and Masters

Women who experience hypertensive pregnancy disorders (HPDs) during pregnancy, mainly pre-eclampsia (PE), are four times more likely to develop hypertension after pregnancy and therefore have an increased risk of developing cardiovascular diseases (CVD) in the future. Pregnancy involves a high level of cardiovascular stress and the development of some complications. This has the potential to increase a woman’s susceptibility to vascular or metabolic disease risk in the future. In this research, pre-eclampsia (PE) and pregnancyinduced hypertension (PIH) were representatives of HPDs and were analysed together for the expression of Growth Differentiation Factor-15 (GDF-15) relative to their healthy agematched controls.

GDF-15 …

Attitudes And Perceptions Towards Privacy And Surveillance In Australia, Aleatha J. Shanley

Theses: Doctorates and Masters

Understanding attitudes towards privacy and surveillance technologies used to enhance security objectives is a complex, but crucial aspect for policy makers to consider. Historically, terrorism-related incidents justified the uptake of surveillance practices. More recently however, biosecurity concerns have motivated nation-states to adopt more intrusive surveillance measures. There is a growing body of literature that supports the public’s desire to maintain privacy despite fears of biological or physical threats.

This research set out to explore attitudes towards privacy and surveillance in an Australian context. Throughout the course of this endeavour, the COVID-19 pandemic emerged bringing with it a variety of track …

Novel Methods For Assessing Pulmonary And Cardio-Vascular Limitations To Aerobic Performance In Older And Younger Populations, Toni Haddad

Theses: Doctorates and Masters

Around the world, life expectancy is increasing, the ageing population is growing, and maintaining quality of life at an older age currently represents a major global challenge. While ‘successful ageing’ relies on a variety of factors, aerobic performance and exercise capacity are key determinants and attenuating their decline is of the utmost importance. The decrease in maximal oxygen consumption is often considered as a major determinant explaining the age[1]related decline in aerobic performance. Yet, multiple physiological systems are impaired with ageing and their contribution to aerobic limitation in older individuals is unclear. Understanding how the limitations to aerobic performance may …

Western Australian Mothers' Experiences Of Breastfeeding Support In Hospital After Birth: A Mixed Methods Study, Justine Van Der Watt

Theses: Doctorates and Masters

Background: The benefits of breastfeeding are well established for infants, mothers and the community, with the World Health Organisation recommending that infants be exclusively breastfed up to six months of age with an achievable goal of 70% globally. Yet in Australia only 15-37% may be reaching that goal. There have also been reported benefits and desires for mothers to be supported with their breastfeeding in order to increase and prolong breastfeeding duration and exclusivity, especially early after birth. However, there is limited research or literature exploring what experiences mothers are having with breastfeeding support, especially during their hospital admissions in …

Prevalence Trends Of Foodborne Pathogens, Bacillus Cereus, Non Stec Escherichia Coli And Staphylococcus Aureus In Ready-To-Eat Foods Sourced From Restaurants, Cafés, Catering And Takeaway Food Premises, Nicole Foxcroft

Theses: Doctorates and Masters

The foodborne pathogens of Bacillus cereus (B. cereus), non STEC Escherichia coli (non STEC E. coli) and Staphylococcus aureus (S. aureus) are currently non-notifiable in Australia unless attributed to a food poisoning outbreak. Due to the lack of data around individual cases and isolations in foods, any changes in prevalence may go undetected. The aim of the study was to determine any changes in prevalence of B. cereus, non STEC E. coli and S. aureus in ready-to-eat foods (RTE) sampled from Western Australian restaurants, cafés, catering facilities, and takeaway food premises during the period July 2009 to June 2022. The …

Spatial And Temporal Variation In Population Genetic Diversity In Seagrasses And The Implications For Resilience, Anna Maria Frouws

Theses: Doctorates and Masters

Seagrass ecosystems are highly productive and fulfil vital roles in the coastal environment as ecosystem engineers and keystone species. Unfortunately, anthropogenic pressures have resulted in a significant loss of seagrass habitat and associated ecosystem services. The complex and unpredictable nature of these pressures, and their increasing extent and intensity globally, makes resilience-based ecosystem management a valuable approach. Resilience-based management requires knowledge on processes enhancing ecosystem resilience and on actions supporting these processes. Genetic diversity, which is the allelic variation among individuals of a population, has been recognised as one of the pillars of resilient ecosystems. Genetic diversity within a population …

Shifting Soil Fungal Communities In Response To Fire And Weed Management In Urban Banksia Woodlands, Aaron Justin Brace

Theses: Doctorates and Masters

This research aims to fill the knowledge gap in soil fungi in the Banksia woodland and emphasizes considering holistic ecosystem dynamics in invasive species management strategies. This is accomplished by surveying soil fungi under different fire regime across the Swan Coastal Plain, and within both in and ex situ herbicide application trials. This is the first such study to investigate the fungal component of the Banksia woodland so thoroughly. Chapter 2 quantified and analysed changes in soil fungal community composition and functional groups over a 50-year fire chronosequence in fire-prone Banksia woodland in southwestern Australia. Effects were variable, but fungal …

Physiological And Histological Effects Of Apigenin And Luteolin On Cytarabine Injected Rats, Omar N. Sultan, Ghada A. Taqa

Baghdad Science Journal

The present study was undertaken to study the effect of apigenin and luteolin on physiological and histological changes in rats treated with cytarabine drugs. Thirty-five albino healthy male adult rats with equal age weighing 250 -300g were enrolled. Rats were randomly divided into seven groups according to the treatment. Group “1” was treated with normal saline and served as the control group. Groups “2,3 and 4” received cytarabine, apigenin, and luteolin respectively, while groups 5, 6, and 7 received a combination of “apigenin + cytarabine”, “luteolin + cytarabine”, and “apigenin + luteolin + cytarabine”, respectively. After one week of treatment, …

A New Approach For Developing Spectrophotometric Determination Of Phenylephrine Drug In Pure, Pharmaceutics And Serum Samples Using Sodium Periodate As Oxidizing Agent Via A Green Method Of Cfia/Merging Zone Technique, Shahad L. Hamed, Bushra B. Qassim

Baghdad Science Journal

The research involved a rapid, automated and highly accurate developed CFIA/MZ technique for estimation of phenylephrine hydrochloride (PHE) in pure, dosage forms and biological sample. This method is based on oxidative coupling reaction of 2,4-dinitrophenylhydrazine (DNPH) with PHE in existence of sodium periodate as oxidizing agent in alkaline medium to form a red colored product at ʎmax (520 nm). A flow rate of 4.3 mL.min-1 using distilled water as a carrier, the method of FIA proved to be as a sensitive and economic analytical tool for estimation of PHE. Within the concentration range of 5-300 μg.mL-1, a calibration curve was …

Molecular Identification Of Methylorubrum Extorquens Using Pcr-Amplified Mxaf Gene Fragments As A Molecular Marker, Anwar A. Maki, Asaad M. R. Al-Taee, Zeenah Weheed Atwan

Baghdad Science Journal

Methylotrophs bacteria are ubiquitous, and they have the ability to consume single carbon (C1) which makes them biological conversion machines. It is the first study to find facultative methylotrophic bacteria in contaminated soils in Iraq. Conventional PCR was employed to amplify MxaF that encodes methanol dehydrogenase enzyme. DNA templates were extracted from bacteria isolated from five contaminated sites in Basra. The gene specific PCR detected Methylorubrum extorquens as the most dominant species in these environments. The ability of M. extorquens to degrade aliphatic hydrocarbons compound was tested at the laboratory. Within 7 days, gas chromatographic (GC) studies of remaining utilized …

Effect Of Nanocapsules And Extract Of Metarhizium Anisopliae In Inhibiting Acetylcholine Esterase Enzyme In Musca Domestica Larvae., Aliaa Abdul Aziz Hammed, Hazim Idan Al Shammari, Soolaf A Kathiar

Baghdad Science Journal

M. domestica is the most important insect that transmit pathogens for diseases in the world. The use of nanotechnology is eco-friendly method in control pests. The study aims to investigate the feasibility of bio-manufacturing nanocapsules of fungal secondary metabolites in order to improve the efficiency of metabolite and assess their inhibitory effect on the acetylcholine esterase enzyme in housefly larvae. An equal mixture of organic solvents, ethyl acetate and dichloromethane, was used to extract the metabolic products of the fungus M. anisopliae, (PEG4000) and chitosan was used in the preparation of nanocapsules. The results of the DLS granular size assay …

Enhanced Photocatalytic Activity Of Cu2o/Zno/Go Nanocomposites On The Methylene Blue Degradation, Frikson Jony Purba, Zuriah Sitorus, Kerista Tarigan, Nurdin Siregar

Baghdad Science Journal

This study synthesized nanocomposite photocatalyst materials from a mixture of Cu2O nanoparticles, ZnO nanoparticles, and graphene oxide (GO) through coprecipitation and hydrothermal methods. This study aims to determine the optimum composition of Cu2O/ZnO/GO nanocomposites in degrading methylene blue. The nanocomposite was synthesized in two steps: 1 the synthesis of Cu2O and ZnO nanoparticles through the coprecipitation method and the preparation of GO through the modified Hummer method. 2 The preparation of Cu2O and ZnO nanoparticles mixtures with GO through the hydrothermal method to form Cu2O/ZnO/GO nanocomposites. The adsorption-photocatalysis process of methylene blue was done with UV light from a halogen …

Relationship Between Serum Nesfatin-1, Adiponectin, Resistin Concentration, And Obesity With Type 2 Diabetes Mellitus, Baydaa Ahmed Abed, Layla Othman Farhan, Ashgan Slman Dawood

Baghdad Science Journal

Diabetes mellitus caused by insulin resistance is prompted by obesity. Neuropeptide Nesfatin-1 was identified in several organs, including the central nervous system and pancreatic islet cells. Nesfatin-1 peptide appears to be involved in hypothalamic circuits that energy homeostasis and control food intake. Adiponectin is a plasma collagen-like protein produced by adipocytes that have been linked to the development of insulin resistance (IR), diabetes mellitus type 2 (DMT2), and cardiovascular disease (CVD). Resistin was first identified as an adipose tissue–specific hormone that was linked to obesity and diabetes. The aim of this study was to estimate the relationship between human serum …

An Integrative Study On Wood-Boring Beetle Larvae: Nutritional Properties, Gut Microbiota And Isolation Of Bioactive Compounds, Shadrack Kibet

All Master’s Theses

Edible insects are increasingly recognized as a solution to several problems faced by mankind, including hunger, malnutrition and microbial infections. Additionally, the gut symbionts of insects, along with their associated metabolites and enzymes, hold promising potential for industrial and pharmaceutical applications. However, the detailed nutritional properties, gut microbiota, and microbial secondary metabolites associated with the gut symbionts of various insects, including wood-boring beetles, have not yet been comprehensively reported. In this current study, the nutritional properties, and the characteristics of the gut microbiota of two wood-boring species (Titocerus jaspideus and Passalus punctiger) collected from two indigenous forested areas was investigated. …

Physicochemical Characterization Of Edible Insect Oils: Insights Into Fatty Acid Composition, Thermal Behavior And Quality Parameters, Eng-Tong Phuah, Yee-Ying Lee, Teck-Kim Tang, Guanghui Li, Shyang-Pei Hong, Syazana Abdullah Lim

ASEAN Journal on Science and Technology for Development

Edible insects are emerging as a sustainable food source due to their high nutritional value. This study employed the three-phase partitioning (TPP) method to extract oils from sago worm (Rhynchophorus ferrugineus), superworm (Zophobas morio), and cricket (Acheta domesticus). The study evaluated and compared the physicochemical properties of these insect oils, including iodine value (IV), peroxide value (PV), p-anisidine value (p-AV), acid value (AV), refractive index (RI), thermal behavior, and fatty acid compositions. Results revealed that the sago worm exhibited the highest oil yield (47.8 ± 2.2%) compared to the superworm (23.8 ± 1.4%) and cricket (6.4 ± 0.6%). Cricket oil …

Application Of Three Of Selected Medicinal Plant Extracts In Brunei Darussalam To Improve Shelf Life Of Poultry Product, Hazirah Abd Hamid, Namasivayam Navaranjan, Zohrah Sulaiman, Aida Maryam Binti Hj Basri

ASEAN Journal on Science and Technology for Development

Abstract. Bioactive compounds from plant derived in food application become increasingly in demand due to natural and nutritionally safe products. Due to some issues concerning food safety on the excessive use of synthetic preservatives; the present study aims to investigate the natural alternatives to synthetic antimicrobial food additives. The chicken meat’s shelf life was studied by adopting bio-based antimicrobial agents using three of selected native medicinal plants; P. betle, A. catechu, and A. tricolor, against common foodborne pathogens; E. coli, P. aeruginosa, S. aureus, and Salmonella spp. The overall results revealed that, all study extracts possessed antioxidant properties by DPPH …

Assessing The Organic Acids-Solubilized Collagen Derived From The Skin Of Unicornfish (Naso Reticulatus Randall, 2011) On Physicochemical And Structural Characteristics: Toward Alternative Source For Industrial Applications, Dayang Fazrianie Harianto, Abdul Aziz Jaziri, Rossita Shapawi, Ruzaidi Azli Mohd Mokhtar, Wan Norhana Md. Noordin, Masmunira Rambli, Nurul Huda

ASEAN Journal on Science and Technology for Development

Converting skin by-product of unicornfish (Naso reticulatus Randall, 2011) into a valuable commodity like collagen is a good strategy to explore alternative sources for land-based animals. Additionally, marine fish collagens are preferable due to their safer and great properties. This project aimed to compare extractability of collagens prepared with three organic acids (i.e., acetic, lactic and citric acids), and their characteristics were also discussed. Collagen extracted with citric acid (CSCUS) (7.10%) had a significantly higher yield (p