Digital Commons Network^™

Structural Basis Of Dna Crossover Capture By Escherichia Coli Dna Gyrase, Marlène Vayssières, Nils Marechal, Long Yun, Brian Lopez Duran, Naveen Kumar Murugasamy, Jonathan M Fogg, Lynn Zechiedrich, Marc Nadal, Valérie Lamour

Faculty, Staff and Students Publications

DNA supercoiling must be precisely regulated by topoisomerases to prevent DNA entanglement. The interaction of type IIA DNA topoisomerases with two DNA molecules, enabling the transport of one duplex through the transient double-stranded break of the other, remains elusive owing to structures derived solely from single linear duplex DNAs lacking topological constraints. Using cryo–electron microscopy, we solved the structure of Escherichia coli DNA gyrase bound to a negatively supercoiled minicircle DNA. We show how DNA gyrase captures a DNA crossover, revealing both conserved molecular grooves that accommodate the DNA helices. Together with molecular tweezer experiments, the structure shows that the …

Heterogeneity And Plasticity Of The Naïve Cd4+ T Cell Compartment, Alia Sajani

Dartmouth College Ph.D Dissertations

Recent studies have illuminated unexpected heterogeneity in the naïve T cell compartment. Using single cell RNA sequencing of mouse naïve CD4⁺ T cells, we identified 5 unique transcriptional subsets within the naïve CD4⁺ T cell compartment. These include a quiescence cluster (T^Q) marked by Klf2, Klf6, Jun and Btg2, a memory-like cluster (T^MEM) marked by Ccr7, Il7r and Tcf7, a TCR reactive cluster (T^TCR) marked by Cd5, Cd6 and Nr4a1, an IFN responsive cluster (T^IFN) marked by Stat1, Irf7 and Ifit1 and …

Cmv Retinitis: An Expert’S Perspective, Caitlyn Kwun, Ba

inSIGHT

Uveitis is a rare inflammatory disease with a prevalence of around 38 per 100,000 people and is the overall 5th leading cause of blindness in the developed world.^1,2 Uveitis is most commonly found in patients younger than 40 years of age, but it can occur in any age group with an etiology that varies within each age demographic.^1,3 While the etiology of certain forms of uveitis are not fully understood, there are some that are autoimmune in nature and others that are associated with systemic diseases such as sarcoidosis.⁴ Uveitis may be inflammatory or infectious. It may …

Insight, Volume 4, Issue 1, 2024

inSIGHT

Contents

8 - The Myopia Epidemic: Exploring the Pathology and Management of the World’s Most Common Eye Disorder
By Gabriella Baldassare

15 - Evolution of Lamellar Keratoplasty
By Ishan Kasat

19 - Bridging the Global Gap of Blindness Through Artificial Intelligence
By Nathan Delacth

24 - CMV Retinitis: An Expert’s Perspective
By Caitlyn Kwun

29 - Macular Holes: Diagnosis, Treatment, and Complications
By Ayra Khan

36 - Chronic Progressive External Ophthalmoplegia
By Bahram Pashaee

39 - Medical Missions, Ethical Considerations, and the Future for Healthcare Delivery in Ophthalmology
By Robert Medina

43 - Shaping the Future of Vision: The Rise …

Chemotherapy Activates Inflammasomes To Cause Inflammation-Associated Bone Loss, Chun Wang, Khushpreet Kaur, Canxin Xu, Yousef Abu-Amer, Gabriel Mbalaviele

2020-Current year OA Pubs

Chemotherapy is a widely used treatment for a variety of solid and hematological malignancies. Despite its success in improving the survival rate of cancer patients, chemotherapy causes significant toxicity to multiple organs, including the skeleton, but the underlying mechanisms have yet to be elucidated. Using tumor-free mouse models, which are commonly used to assess direct off-target effects of anti-neoplastic therapies, we found that doxorubicin caused massive bone loss in wild-type mice, a phenotype associated with increased number of osteoclasts, leukopenia, elevated serum levels of danger-associated molecular patterns (DAMPs; e.g. cell-free DNA and ATP) and cytokines (e.g. IL-1β and IL-18). Accordingly, …

Upstarts T3: An Asynchronous And Cohort-Based Entrepreneurship Train-The-Trainer Program, Surin Kim, Maria Rosario T. De Guzman, Kieu-Anh Do, Irene Padasas, Claire Nicholas, Olivia Kennedy, Anna Erdmann, Andy Larson

Journal of Extension

UpStarts is a reverse-mentoring youth program designed to foster an entrepreneurial mindset among participants and a sense of connection between youth and adults. Effective facilitators are critical to the success of the program. This paper describes an asynchronous, cohort-based virtual train-the-trainer (T3) program for potential UpStarts facilitators that was developed to navigate the challenges posed by the COVID pandemic. Participants of the initial UpStarts T3 program reported positive feedback for the training and a high sense of efficacy in implementing UpStarts in their communities. The current T3 model is emerging as a promising means of preparing facilitators to implement UpStarts …

Bacterial And Viral Co-Infections In Aquaculture Under Climate Warming: Co-Evolutionary Implications, Diagnosis, And Treatment, S Vega-Heredia, I Giffard-Mena, M Reverter

School of Biological and Marine Sciences

Climate change and the associated environmental temperature fluctuations are contributing to increases in the frequency and severity of disease outbreaks in both wild and farmed aquatic species. This has a significant impact on biodiversity and also puts global food production systems, such as aquaculture, at risk. Most infections are the result of complex interactions be - tween multiple pathogens, and understanding these interactions and their co-evolutionary mechanisms is crucial for developing effective diagnosis and control strategies. In this review, we discuss current knowledge on bacteria-bacteria, virus-virus, and bacterial and viral co-infections in aquaculture as well as their co-evolution in the …

The World Health Organization Was Born As A Normative Agency: Seventy-Five Years Of Global Health Law Under Who Governance, Lawrence O. Gostin, Benjamin Mason Meier, Safura Abdool Karim, Judith Bueno De Mesquita, Gian Luca Burci, Danwood Chirwa, Alexandra Finch, Eric A. Friedman, Roojin Habibi, Sam F. Halabi, Tsung-Ling Lee, Brigit Toebes, Pedro Villarreal

Georgetown Law Faculty Publications and Other Works

The World Health Organization (WHO) was born as a normative agency and has looked to global health law to structure collective action to realize global health with justice. Framed by its constitutional authority to act as the directing and coordinating authority on international health, WHO has long been seen as the central actor in the development and implementation of global health law. However, WHO has faced challenges in advancing law to prevent disease and promote health over the past 75 years, with global health law constrained by new health actors, shifting normative frameworks, and soft law diplomacy. These challenges were …

Leveraging 4-H To Address Emergent Health Needs During The Covid-19 Pandemic: Epidemiology Pilot Project, Anne Marie Iaccopucci, Marcel Horowitz, Dorina Espinoza, Roshan Nayak

Journal of Extension

4-H academics responded to the Covid pandemic by rapidly adapting CDC and other resources for virtual delivery. A statewide epidemiology project was taught to 48 youth with the goal of minimizing fears and confusion, increasing prevention measures, leveraging current topics for education, and bolstering the social-emotional health of youth participants. Results indicated that youth enjoyed the project and adopted behaviors to reduce the spread of infectious diseases, such as increased mask-wearing.

Typhlitis In A Neutropenic Patient, Alice He Bs, Wern Lynn Ng Md, Lay She Ng Md, Si Yuan Khor Md, Chandi Garg Md

Tower Health Research Day

No abstract provided.

Chronic Pneumonia In A 1-Year-Old Male, Saritha Beauchamp, Irene Chern, Emily Souder

Tower Health Research Day

No abstract provided.

Medical-Dental Staff Topics, 1988, V22 N2, February, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Hospital, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

Medical-Dental Staff Topics, 1988 May, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Medical Center, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

This issue introduces a new format and also includes information on the renaming of the hospital to St. Luke's Medical Center and letters from the chief of staff and hospital president.

Medical-Dental Staff Topics, 1988 October, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Medical Center, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

The Painful Knee: A Case Of Prosthetic Joint Infection In Hospitalized Patient With Esbl-Producing Klebsiella Uti, Shawn Mathew, Dhaval Patel

Tower Health Research Day

No abstract provided.

Hiding In Plain Sight; Dermatologic Manifestation Of A Systemic Disease, Christopher Ignatz, David Young Do, Brian Chwiecko Md

Tower Health Research Day

No abstract provided.

Debone The Fish Carefully: A Case Of Vibrio Vulnificus Septicemia, Simranpreet Singh, Devi Parvathy Jyothi Ramachandran Nair, Zohaib Yousaf, Tahminah Kausar, Hamzah Moh'd

Tower Health Research Day

No abstract provided.

Medical-Dental Staff Topics, 1987, V21 N1, January, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Hospital, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

Medical-Dental Staff Topics, 1987, V21 N3, March, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Hospital, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

Medical-Dental Staff Topics, 1987, V21 N5, May, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Hospital, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

Medical-Dental Staff Topics, 1987, V21 N9, September, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Hospital, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

Medical-Dental Staff Topics, 1985, V19 N7, July, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Hospital, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

Medical-Dental Staff Topics, 1985, V19 N8, August, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Hospital, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

Medical-Dental Staff Topics, 1986, V20 N9, October, Advocate Health - Midwest

Historical Documents - Combined

St. Luke's Hospital, Milwaukee, WI: An issue of "Medical-Dental Staff Topics". This was an internal publication for ASLMC staff members reporting on news, research, events, and staffing updates. Includes a staff calendar and information on continuing education opportunities.

The Perception Of Official Housing Policies For Women With Medically Assisted Treatment, Vickie Lynn Roberson

Walden Dissertations and Doctoral Studies

Opioid overdose is the leading cause of death among the homeless, with limited access to housing, food, security, and medically assisted treatment (MAT). The women on MAT are affected because they may not be readily accepted into the housing community if considered non-abstinent, unlike other substance users who would not have those problems. There previously had not been research on how housing for women was affected when on MAT or any housing facilities that have established MAT protocol. In this generic qualitative design, 10 female participants on MAT who needed housing were interviewed using semi-structured interviews. Bandura's social learning theory …

Reply To Kidd Et Al., “Inconsistencies Within The Proposed Framework For Stabilizing Fungal Nomenclature Risk Further Confusion”, Sybren De Hoog, Thomas J Walsh, Sarah A Ahmed, Ana Alastruey-Izquierdo, Barbara D Alexander, Maiken Cavling Arendrup, Esther Babady, Feng-Yan Bai, Joan-Miquel Balada-Llasat, Andrew Borman, Anuradha Chowdhary, Andrew Clark, Robert C Colgrove, Oliver A Cornely, Tanis C Dingle, Philippe J Dufresne, Jeff Fuller, Jean-Pierre Gangneux, Connie Gibas, Heather Glasgow, Yvonne Graser, Jacques Guillot, Andreas H Groll, Gerhard Haase, Kimberly Hanson, Amanda Harrington, David L Hawksworth, Randall T Hayden, Martin Hoenigl, Vit Hubka, Kristie Johnson, Julianne V Kus, Ruoyu Li, Jacques F Meis, Michaela Lackner, Fanny Lanternier, Sixto M Leal, Francesca Lee, Shawn R Lockhart, Paul Luethy, Isabella Martin, Kyung J Kwon-Chung, Wieland Meyer, M Hong Nguyen, Luis Ostrosky-Zeichner, Elizabeth Palavecino, Preeti Pancholi, Peter G Pappas, Gary W Procop, Scott A Redhead, Daniel D Rhoads, Stefan Riedel, Bryan Stevens, Kaede Ota Sullivan, Paschalis Vergidis, Emmanuel Roilides, Amir Seyedmousavi, Lili Tao, Vania A Vicente, Roxana G Vitale, Qi-Ming Wang, Nancy L Wengenack, Lars Westblade, Nathan Wiederhold, Lewis White, Christina M Wojewoda, Sean X Zhang

Faculty, Staff and Student Publications

No abstract provided.

Vaccine Effectiveness Against Influenza A-Associated Hospitalization, Organ Failure, And Death: United States, 2022-2023, Nathaniel M Lewis, Yuwei Zhu, Ithan D Peltan, Manjusha Gaglani, Tresa Mcneal, Shekhar Ghamande, Jay S Steingrub, Nathan I Shapiro, Abhijit Duggal, William S Bender, Leyla Taghizadeh, Samuel M Brown, David N Hager, Michelle N Gong, Amira Mohamed, Matthew C Exline, Akram Khan, Jennifer G Wilson, Nida Qadir, Steven Y Chang, Adit A Ginde, Nicholas M Mohr, Christopher Mallow, Adam S Lauring, Nicholas J Johnson, Kevin W Gibbs, Jennie H Kwon, Cristie Columbus, Robert L Gottlieb, Catherine Raver, Ivana A Vaughn, Mayur Ramesh, Cassandra Johnson, Lois Lamerato, Basmah Safdar, Jonathan D Casey, Todd W Rice, Natasha Halasa, James D Chappell, Carlos G Grijalva, H Keipp Talbot, Adrienne Baughman, Kelsey N Womack, Sydney A Swan, Elizabeth Harker, Ashley Price, Jennifer Decuir, Diya Surie, Sascha Ellington, Wesley H Self, Investigating Respiratory Viruses In The Acutely Ill (Ivy) Network

Faculty, Staff and Students Publications

BACKGROUND: Influenza circulation during the 2022-2023 season in the United States largely returned to pre-coronavirus disease 2019 (COVID-19)-pandemic patterns and levels. Influenza A(H3N2) viruses were detected most frequently this season, predominately clade 3C.2a1b.2a, a close antigenic match to the vaccine strain.

METHODS: To understand effectiveness of the 2022-2023 influenza vaccine against influenza-associated hospitalization, organ failure, and death, a multicenter sentinel surveillance network in the United States prospectively enrolled adults hospitalized with acute respiratory illness between 1 October 2022, and 28 February 2023. Using the test-negative design, vaccine effectiveness (VE) estimates against influenza-associated hospitalization, organ failures, and death were measured by …

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Perceptions Of Dengue Risk And Acceptability Of A Dengue Vaccine In Residents Of Puerto Rico, Coral Rosado-Santiago, Carmen L. Pérez-Guerra, Nicole M. Velez-Agosto, Claudia Colón-Burgos, Karla M. Marrero-Santos, Susanna K. Partridge, Amy E. Lockwood, Cathy Young, Steve H. Waterman, Gabriela Paz-Bailey

Psychological Science Faculty Publications

Dengvaxia is the first dengue vaccine recommended in the United States (U.S.). It is recommended for children aged 9–16 y with laboratory-confirmed previous dengue infection and living in areas where dengue is endemic. We conducted focus groups with parents and in-depth interviews with key informants (i.e. practicing pediatricians, physicians from immunization clinics, university researchers, and school officials) in Puerto Rico (P.R.) to examine acceptability, barriers, and motivators to vaccinate with Dengvaxia. We also carried out informal meetings and semi-structured interviews to evaluate key messages and educational materials with pediatricians and parents. Barriers to vaccination included lack of information, distrust toward …

A Novel Lentiviral Vector-Based Approach To Generate Chimeric Antigen Receptor T Cells Targeting, Pappanaicken R Kumaresan, Sebastian Wurster, Karishma Bavisi, Thiago Aparecido Da Silva, Paul Hauser, Jordan Kinnitt, Nathaniel D Albert, Uddalak Bharadwaj, Sattva Neelapu, Dimitrios P Kontoyiannis

Faculty, Staff and Student Publications

Invasive aspergillosis (IA) is a common and deadly mold infection in immunocompromised patients. As morbidity and mortality of IA are primarily driven by poor immune defense, adjunct immunotherapies, such as chimeric antigen receptor (CAR) T cells, are direly needed. Here, we propose a novel approach to generate Aspergillus fumigatus (AF)-CAR T cells using the single-chain variable fragment domain of monoclonal antibody AF-269-5 and a lentiviral vector system. These cells successfully targeted mature hyphal filaments of representative clinical and reference AF isolates and elicited a potent release of cytotoxic effectors and type 1 T cell cytokines. Furthermore, AF-CAR T cells generated …