Digital Commons Network^™

Effect Of Short-Term Exposure To Pesticide Mixture On Tissue Architecture, Body Fluid Conditions, Nitrative Stress And Osmoregulation Of American Oyster, Crassostrea Virginica, Asif Ahmed

Theses and Dissertations

Pesticides are used widely to control weeds and pests in agricultural settings but harm non-target aquatic organisms. We examined the effect of short-term exposure (one week) to pesticide mixtures (low dose: 0.5 μg/L Roundup®, 0.5 μg/L 2,4-D, and 0.4 μg/L atrazine; high dose: 1 μg/L Roundup®, 1 μg/L 2,4-D, and 0.8 μg/L atrazine) on American oyster (Crassostrea virginica, an important shellfish and seafood species) under controlled laboratory conditions. Histological analyses demonstrated the atrophy in oysters' gills, digestive glands, and connective tissue. The protein concentrations and glucose levels dropped significantly in the high-dose treatment. The number of hemocytes in connective tissue …

Cell Type-Specific Production Of Amyloid Precursor Protein During Infection Is Dependent On The Strain Of Herpes Simplex Virus 1, Mia Elias

Honors Theses

Cleavage of amyloid precursor protein (APP) generates the antimicrobial peptide amyloid beta (Aβ), the accumulation of which is linked to Alzheimer’s Disease (AD) pathogenesis. Whether processing of APP contributes to anti-viral responses is debated. Herpes Simplex Virus Type 1 (HSV-1), a neurotropic double-stranded deoxyribonucleic acid (DNA) virus, establishes lifelong latency and can cause a range of outcomes, from asymptomatic infection to herpetic encephalitis (HSE). Whether disparate outcomes are dependent on HSV-1 strain is unknown. Using three differentially virulent HSV-1 strains, we compared the regulation of APP-associated proteolytic processing in both a corneal infection murine model and an in vitro model. …

Screening For Potential Swine-Origin Probiotics With Antimicrobial Activities, Christina Lim

Animal Science Undergraduate Honors Theses

The prevalence of antibiotic resistant bacteria is a leading issue worldwide. Recently, there was a new law banning the use of antibiotics within the swine industry. For decades swine producers have mixed subtherapeutic amounts of antibiotics in swine feed to prevent potential infections and to increase growth rate. However, due to the constant exposure of antibiotics to these ever-evolving pathogens, the population of antibiotic resistant bacteria has increased exponentially. Currently, the swine industry is scrambling to find a suitable alternative to antibiotics that possesses antimicrobial activity and potentially promotes growth. The objective of this study was to investigate the ability …

Water Justice And Equity: The Disproportionate Effects Of Pollution On Black, Indigenous, And Other Communities Of Color, Rae Cade

Theses, Dissertations and Culminating Projects

Access to clean drinking water is a basic human right, regardless of an individual’s background. Yet, discriminatory practices in the United States have disproportionately burdened Black, Indigenous, and other people of color (BIPOC) with contaminated sources of water. Understanding how and why water is disproportionately contaminated in areas with increased diversity, and identifying and targeting areas of environmental injustice, are essential steps for ensuring future water equity. Here, I examine the drivers of inequitable access to clean drinking water within the northeastern United States. In chapter one, I look at the historical drivers of land inequity within the United States. …

Comparative Population Genomics Of Bloodstream-Derived Staphylococcus Aureus From Disparate Geographic Origins In The United States, Milan Pfister

Legacy Theses & Dissertations (2009 - 2024)

Staphylococcus aureus is a commensal and an opportunistic pathogen that causes a variety of diseases in humans, from minor skin and soft tissue infections to life-threatening invasive diseases. The emergence and spread of multidrug-resistant and methicillin-resistant S. aureus (MRSA) complicate treatment options, which can lead to significant morbidity and mortality of patients. In this study, we compared the clonal lineages and genomic features of bloodstream-derived S. aureus populations from different locations. We leveraged 957 previously published genomes derived from bloodstream infections across six hospitals located in Illinois, Minnesota, Missouri, New Hampshire, and New York, USA. We carried out pan-genome analysis, …

Species Distribution And Logistic Regression Analysis Of Nosocomial Legionella Detection And Infection In New York State, 2017 – 2023, Roxann Brna

Legacy Theses & Dissertations (2009 - 2024)

Healthcare facilities are high-risk areas for legionellosis infections, as they have large, complex potable water systems ideal for Legionella proliferation, and they host susceptible populations. New York State regulation 10 NYCRR § 4-2: Protection Against Legionella in Healthcare Facilities was implemented in July 2016 to reduce healthcare-acquired legionellosis. This regulation mandated environmental surveillance of healthcare facilities’ potable water systems via culture. Data from these environmental reports were paired with data for cases of healthcare-acquired legionellosis infections for the years 2017-2023. Using these data, geographic Legionella species and serogroup distributions were described, and logistic regressions evaluated facility attributes associated with potable …

State Policies On Maternal And Infant Health In The United States, Heeun Kim

Legacy Theses & Dissertations (2009 - 2024)

In the United States (US), state-level policies are critical structural determinants of maternal and infant health outcomes. State policies profoundly affect prenatal care utilization and pregnancy outcomes, particularly among marginalized pregnant persons with stigmatized health conditions. In this three-paper dissertation, I focus on two closely interrelated policies that aim to address significant public health concerns in the US: prenatal syphilis screenings and the criminalization of prenatal substance use. My research revolves around two central questions: First, do these policies effectively improve the pregnancy outcomes of high-risk populations, or do they cause unintended consequences? Second, if challenges in policy design and …

The Role Of B Cell Activation State And Sex In Aryl Hydrocarbon Receptor Mediated Induction Of Chemokine Receptor 9 And Alpha4beta7 Expression In Vitro, Logan Bauerle

Master's Theses

Defense of mucosal tissues from microbial infection and allergy is reliant on continual production of antibodies. The aryl hydrocarbon receptor (AhR) is known to regulate B cell development and is associated with suppression of systemic humoral immunity. Recent attention has been paid to the role of the AhR in altering expression of cell adhesion molecules (CAMs). B cells express CAMs and chemokine receptors to migrate around the body for localized secretion of antibodies. AhR agonists promote B cell migration to the small intestine through upregulation of chemokine receptor 9 (CCR9) and integrin α₄β₇. Both the AhR …

Nebline, May 2024

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Weed Control in Pastures and Hayfields by John Nelson

Navigating Nutrition Information: Separating Fact from Fiction by Alyssa Havlovic

Recipe of the Month: Pepper Steak Sandwich by Alyssa Havlovic

Care for New Bedding Plants by Sarah Browning

Garden Guide: Things to Do This Month

Cash Rent Survey

No Matter Where You Live, Submit a Request Before You Dig! by Becky Schuerman

4-H Achievement Celebration

8 Lancaster County 4-H’ers Modeled Their Sewn Garments at Omaha Fashion Week

4-H Events Open to All Youth

Support 4-H by Eating Out, May 1 & 2

Support 4-H During “Give to Lincoln Day,” May 30 …

An Examination Of Missing Person Social Media Engagement Through Data Mining And Experimentation: An Application Of The Crisis And Emergency Risk Communication Model, Cailin M. Kuchenbecker

Communication (PhD) Dissertations

According to the Federal Bureau of Investigation (FBI), approximately 600,000 individuals are reported missing each year in the United States (2022). When missing person cases do not meet alert (e.g., AMBER) criteria, law enforcement often utilize social media to crowdsource information to ultimately return the missing home. Therefore, guided by the crisis and emergency risk communication model (CERC; Reynolds & Seeger, 2005) and its recently clarified propositions (Miller et al., 2021), the purpose of this dissertation was to (a) identify strategies law enforcement use to crowdsource missing person information and (b) experimentally test message characteristics that facilitate prosocial sharing of …

Investigating The Antibacterial Properties Of Capsaicin In Synergy With Antibiotics Against A. Baumannii, Jack Guffey

Undergraduate Theses

As the ever-increasing issue of antibiotic resistance looms, naturally derived compounds have been increasingly investigated as possible synergists with antibiotics. One of these compounds, capsaicin, is a potent vanilloid that is the dominant cause of Capsicum peppers’ spiciness. Capsaicin’s intrinsic ability to inhibit a wide array of bacterial growth has been well documented. The focal organism of this study, Acinetobacter baumannii, has become an increasingly common hospital acquired infection due to its persistence on fomites. Capsaicin, however, is able to inhibit this growth at higher concentrations as well as reduce the necessary minimum inhibitory concentration while decreasing biofilm formation …

A Revised Synthesis Of The Cationic Lipids Dmri And Mlri, Benjamin A. Kostic

College of Arts & Sciences Senior Theses

Lipid nanoparticles (LNPs) are an increasingly utilized method of intracellular delivery for nucleic acids. The COVID-19 vaccines from Moderna and Pfizer-BioNTech have used LNP technology to combat the recent global SARS-CoV-2 pandemic, saving millions of lives.^1,2 These vaccines consist of a lipid formulation that binds to the anionic phosphate groups of mRNAs and serves as an effective way to transport the nucleic acid across membranes into cells.⁹The mRNA encodes a viral protein. When introduced to cells, the viral protein is produced so that the immune system then can generate an appropriate antibody response.³ However, this approach …

Spatiotemporal Assessment Of Pathogenic Leptospira In Subtropical Coastal Watersheds, Ronell Bridgemohan, Matthew J. Deitch, Emily Harmon, Matt R. Whiles, P Christopher Wilson, Eban Bean, Puran Bridgemohan, Joseph H. Bisesi, Jodel Nicholas, Aaden Redhead, Dave S. Bachoon

Faculty and Staff Works

The World Health Organization classifies leptospirosis as a significant public health concern, predominantly affecting impoverished and unsanitary regions. By using the Pensacola Bay System as a case study, this study examines the underappreciated susceptibility of developed subtropical coastal ecosystems such as the Pensacola Bay System to neglected zoonotic pathogens such as Leptospira. We analyzed 132 water samples collected over 12 months from 44 distinct locations with high levels of Escherichia coli (>410 most probable number/100 mL). Fecal indicator bacteria (FIB) concentrations were assessed using IDEXX Colilert-18 and Enterolert-18, and an analysis of water physiochemical characteristics and rainfall intensity was …

Analysis Of Antibiotic Sensitivity Patterns In Pasteurellaceae Family Isolates: A Microbial And Molecular Investigation, Jillian Barron

Honors Scholar Theses

Bacteria in the Pasteurellaceae family are known to cause disease in both human and animal species. Like all species of bacteria, the pathogens in the Pasteurellaceae family evolve and acquire antimicrobial-resistant traits. Antimicrobial resistance of bacteria is a significant public health concern, as treatment of resistant pathogens becomes more difficult, if not impossible, with current medical capabilities. In this project, phenotypic and genotypic analyses were performed on Pasteurellaceae family isolates sourced from the Connecticut Veterinary Medical Diagnostic Laboratory. Phenotypic antibiotic sensitivity patterns of each isolate were assessed against a panel of antibiotics through a Kirby-Bauer disk diffusion test. Disk diffusion …

The Association Between Aerobic Exercise And Changes In Hdl Quality Induced By Egg Consumption – A Post-Hoc Analysis Of A Randomized, Controlled, Crossover Dietary Intervention Study, Rachael Woodruff, Catherine J. Andersen

Honors Scholar Theses

Abstract

Objective: Daily intake of whole eggs and aerobic exercise have independently been shown to increase high-density lipoprotein cholesterol (HDL-C) and measures of HDL function, suggesting cardioprotective effects of these lifestyle interventions. However, it is unknown whether exercise volume can synergistically or differentially impact diet responses and egg effects on HDL. Therefore, we evaluated whether exercise volume was associated with distinct effects of consuming different egg-based diets on measures of HDL size, function, and composition.

Methods: Twenty-six healthy men and women completed a 16-week randomized, controlled, crossover intervention study which aimed to investigate the effects of egg-free, egg-white, and whole-egg …

Per- And Polyfluoroalkyl Substance Exposure Risks In Us Carceral Facilities, 2022, Lindsay Poirier, Derrick Salvatore, Phil Brown, Alissa Cordner, Kira Mok, Nicholas Shapiro

Statistical and Data Sciences: Faculty Publications

Objectives. To assess the US incarcerated population’s risk of exposure to per- and polyfluoroalkyl substances (PFASs). Methods. We assessed how many of the 6118 US carceral facilities were located in the same hydrologic unit code watershed boundaries as known or likely locations of PFAS contamination. We conducted geospatial analyses on data aggregated from Environmental Protection Agency databases and a PFAS site tracker in 2022 to model the hydrologically feasible known and presumptive PFAS contamination sites for nearly 2 million incarcerated people. Results. Findings indicate that 5% (∼310) of US carceral facilities have at least 1 known source of PFAS contamination …

A Comparative Analysis Of Hiv/Aids In France And The United States: Historical Context And Preventative Actions, Rebecca A. Liebsack

Honors Program: Senior Projects (Public)

The HIV/AIDS pandemic is the result of transmission of a zoonotic disease known as simian immunodeficiency virus. The pandemic has had profound social and economic consequences and continues to be present today. France and the United States’ response to the discovery of HIV will be compared and the impact that HIV/AIDS had on their countries and future responses. They had rather similar responses, however, the United States had a slower initial response compared to France. Both had similar takeaways such as aiming at improving prevention and utilizing tactics developed during the start of the pandemic like frequent testing and vaccines.

Evaluation Of Wogonin And Chrysin In Alleviating Mycophenolate-Induced Diarrhea And Its Pharmacokinetics Profile, Abasifreke Sunday Benson

Theses (2016-Present)

Medication-induced diarrhea is a common adverse effect experienced by patients taking immunosuppressant medications used in Organ transplants. However, Mycophenolate mofetil (MMF) is the first-line drug to prevent organ transplant rejection. (Van Gelder & Hesselink, 2015) . MMF’s mechanism of action involves inhibition of inosine-5'-monophosphate dehydrogenase. It is used for the treatment and management of a variety of autoimmune diseases as well as to prevent organ rejection in patients with bone marrow and solid organ transplants; however, over 50% of its adverse effects have been reported to be diarrhea. (Arslan et al., 2007; Farooqi et al., 2020). However, few options exist …

Enhanced Resolution Profiling In Twins Reveals Differential Methylation Signatures Of Type 2 Diabetes With Links To Its Complications., Colette Christiansen, Louis Potier, Tiphaine C. Martin, Sergio Villicaña, Juan E. Castillo-Fernandez, Massimo Mangino, Cristina Menni, Pei-Chien Tsai, Purdey J. Campbell, Shelby Mullin, Juan R. Ordoñana, Olga Monteagudo, Perminder S. Sachdev, Karen A. Mather, Julian N. Trollor, Kirsi H. Pietilainen, Miina Ollikainen, Christine Dalgård, Kirsten Kyvik, Kaare Christensen, Jenny Van Dongen, Gonneke Willemsen, Dorret I. Boomsma, Patrik K E Magnusson, Nancy L. Pedersen, Scott G. Wilson, Elin Grundberg, Tim D. Spector, Jordana T. Bell

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Type 2 diabetes (T2D) susceptibility is influenced by genetic and environmental factors. Previous findings suggest DNA methylation as a potential mechanism in T2D pathogenesis and progression.

METHODS: We profiled DNA methylation in 248 blood samples from participants of European ancestry from 7 twin cohorts using a methylation sequencing platform targeting regulatory genomic regions encompassing 2,048,698 CpG sites.

FINDINGS: We find and replicate 3 previously unreported T2D differentially methylated CpG positions (T2D-DMPs) at FDR 5% in RGL3, NGB and OTX2, and 20 signals at FDR 25%, of which 14 replicated. Integrating genetic variation and T2D-discordant monozygotic twin analyses, we identify …

The Beast With Many Heads: Situational And Dispositional Differences In Physiological, Subjective, And Behavioral Responses To Social Rejection, Ellyn C. Maese

Psychology Theses, Dissertations, and Student Creative Activity

Threats to humans’ deep-rooted social needs are associated with strong physiological, emotional, and behavioral responses that are linked to systems for physical pain responses. Yet, prior research has also revealed inconsistencies in responses to social threats, which may be related to differences in situational or dispositional factors. In exploration of these potential explanations, the proposed research aims to investigate physiological, subjective, and behavioral responses to two distinct types of social threat experiences. This study will involve analyzing data collected from a sample of 127 students at a midwestern state university who participated in a laboratory experiment in which they faced …

Methenamine Prophylaxis Reduces Recurrent Urinary Tract Infections, Salwan Al Hani, Matthew Sims

Posters

• Recurrent urinary tract infections (UTIs) are the most common outpatient infection in the US and are often managed with antibiotics in clinical practice.
• A recurrent UTI is defined as 2 or UTIs each 6 months or 3 or more UTIs each year.
• Methenamine is an antiseptic compound approved by the FDA to prevent rUTIs but is seldom used in clinical practice.
• Under acidic conditions, methenamine is converted to 6 molecules of formaldehyde, a compound which prevents bacterial growth.
• This study seeks to determine the effectiveness of methenamine for prevention of recurrent UTIs.

The Hiv Care Continuum - A Quality Improvement Project To Reconnect With Hiv-Positive Patients Lost To Follow-Up Care, Kate Romero, Janet Lombardi, Carmen Demarco, Paul C. Johnson

Posters

• Human Immunodeficiency Virus (HIV): incurable illness transmitted through sex, blood, and trans placentally • Consistent management of HIV prevents progression to AIDS.
• 2013 study à HIV-positive patients who failed to attend their follow-up visit within 4-6 months of the prior visit were more likely to have virologic failure.
• Not much published on barriers to care, but one prior study found barriers shown in Figure 2 below – What are barriers to care faced by HIV-positive patients in Southeast Michigan?

Hiv Diagnosis And Linkage To Care In The Emergency Department, Gabrielle Rowlison, Christopher F. Carpenter

Posters

• It is estimated that 1.2 million people are living with human immunodeficiency virus (HIV) in the United States, and that 14% of this population are unaware of their status.
• Approximately 40% of community transmissions happen between people who do not know they have HIV.
• Current CDC recommendations are that anyone seeking treatment for a sexually transmitted infection (STI) be routinely screened for HIV.
• The Emergency Department (ED) is an ideal place to test for HIV, especially in areas with underinsured populations and high rates of ED visits.
• The HIV testing rate in Corewell Health (CH) East’s ED was previously found …

A Meta-Analysis For Laboratory Diagnostics For Coccidioidomycosis, Mary C. Cowen

Biological Sciences Undergraduate Honors Theses

Coccidioidomycosis, commonly referred to as Valley Fever, is a fungal infection found in arid regions of the southwestern United States and Mexico. Infection occurs through inhalation of airborne spores from Coccidioides species, Coccidioides immitis and/or Coccidioides posadasii, and proceeds in both pulmonary and disseminated fashions. Approximately 60% of patients with coccidioidomycosis remain asymptomatic, while 40% will experience symptoms. Within the literature, there are no papers that summarize sensitivity and specificity values between different tests; therefore, this paper presents sensitivity and specificity results across different tests and companies (Meridian, IMMY, and MiraVista).

A Multimodal Approach To Decreasing Hesitancy Of The Influenza Vaccine In The Family Care Setting, Carley J. Ellenberger

Evidence-Based Practice Project Reports

The uptake of the influenza vaccine is the most effective and proactive action an individual can take to defend themselves against the influenza outbreak each season; therefore, it is recommended that everyone at least six months of age and older should receive the annual flu vaccine (CDC, 2022; WHO, 2023). Unfortunately, influenza vaccine hesitancy is demonstrated on a global level affecting the uptake compliance annually each influenza season despite the millions of people that experience influenza symptoms annually (CDC, 2022, WHO, n.d.; Healthy People 2030, n.d.). The PICOT question for this project was in adults, over the age of 18, …

The Use Of Whole Blood In Increasing The Survivability Of Combat Wounded Troops From 1940 To 1953, Devin Sweet

LSU New Orleans Theses and Dissertations

Humans have been experimenting with new ways to kill each other for millennia, and for just as long, doctors have been experimenting with methods to improve survivability of those wounded. The 20^th century was one of the bloodiest period in human history; war claimed over one hundred million lives. With the scourge of disease more easily managed, the medical communities came to address the one wound that historically cost most lives – bleeding to death. Shock and exsanguination can kill a person within thirty seconds to two minutes, and finding ways to mitigate that outcome has been the subject …

Pipecolic Acid And Novel Insights Into Cerebral Malaria, Akua E. Mensah

Theses and Dissertations

Cerebral malaria (CM), a severe manifestation of Plasmodium infection, prompts our investigation into the nuanced role of pipecolic acid in its pathophysiology. To unravel the molecular intricacies, we conducted in vitro lysine labeling techniques of mice infected with P. berghei ANKA parasites, and human P. falciparum grown in vitro, aiming to discern the impact of Plasmodium on pipecolic acid production. Previous observations indicated an elevation in pipecolic acid levels correlating with neurological decline in children with CM. In our study, confirming elevated pipecolic acid presence in the plasma and brain tissues of CM patients and the animal model of CM, …

How Monitoring Food Is Protecting People, Grace Sugden

Capstone Projects and Master's Theses

While the issue of food safety has always been a concern for humanity, what that looks like has changed drastically over time. Instead of trying to judge if a stream has safe drinking water, consumers are now faced with deciding which preservatives and pesticides they expose themselves to. With the multitude of United States agencies tasked with protecting the public, standards and quality control processes should be transparent and efficient. Since the creation of the Bureau of Chemistry, numerous acts have been passed that regulate ingredients, processing procedures, and minimize contamination risks. When a spoiled product enters the food web, …

Characterizing The Role Of Pa5189 Of Pseudomonas Aeruginosa In Deletion And Overexpression Mutants, Seh Na Mellick

Theses/Capstones/Creative Projects

In the context of rising multidrug resistance in biofilm-forming pathogens like Pseudomonas aeruginosa, this study investigates the role of the understudied transcription factor PA5189 in antibiotic resistance and biofilm formation. PA5189 deletion and overexpression mutants were created in a parent P. aeruginosa strain using pEX18Tc-based recombinant suicide vectors, with genotypic verification of putative triparental conjugants achieved through restriction digestion and PCR. The study revealed that PA5189 overexpression significantly increases resistance to commonly used broad spectrum antibiotics such as ciprofloxacin and imipenem. Additionally, differential expression of PA5189 was found to notably affect biofilm formation, with variations contingent on the nutrient …

Community Health Assessment Collaboration: Implementing A Joint Assessment Between A Healthcare System And A Local Health Department, Briar L. Deen

Dissertations: Doctor of Public Health

Non-profit hospitals and local health departments (LHDs) often complete community health assessments (CHAs) to help understand the needs of their community and develop implementation strategies to address the community needs. Hospitals and LHDs in the same community often develop separate CHAs with similar results leading to overlap in work and funds. This project aimed to address this issue in the Dallas-Fort Worth Metroplex by convening one local health department, Denton County Public Health, and one non-profit healthcare system, Texas Health Resources, to leverage resources from each other to build a joint CHA. The Mobilizing for Action through Planning and Partnerships …