The Identification And Characterization Of Novel And Improved Antigens For Next-Generation Pertussis Vaccines, Gage Michael Pyles

Graduate Theses, Dissertations, and Problem Reports (ETD)

Whooping cough, or pertussis, is a highly infectious respiratory disease caused by infection with the Gram-negative bacterial pathogen Bordetella pertussis. Pertussis affects all age groups and populations; however, it is most severe in infants who are too young to receive vaccinations and remains a global health concern with more than­­­­ 200,000 cases reported globally in 2023. Vaccines against pertussis have been available in the United States since the 1940’s with the development and widespread use of whole cell pertussis vaccines. Following their introduction, the incidence of pertussis was controlled and limited to fewer than 5,000 cases per year. Despite …

Power Safety Plan Checklist As An Intervention In The Operating Room, Scott John Fankhauser

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Power Safety Plan Checklist as an Intervention in the Operating Room

Scott J. Fankhauser

Background: Intraoperative power failures (IOPF) force anesthesia providers to quickly make decisions to prevent patient harm when equipment does not operate as designed. Familiarity with alternative methods is critical during this decision process to deliver high-quality care during IOPF events. Purpose: The purpose of this project was to assess the impact of a checklist on anesthesia providers’ perceptions regarding continuation of care during an IOPF. Interventions: Pre- and post-intervention surveys were conducted during a simulation and education setting for annual competencies. Methods: The study …

Changes In Psychiatric Diagnosis Associated With Sars-Cov-2 Infection And Predicting The Development Of New Psychiatric Illness In Covid Patients By Using Machine Learning Approach: A Study Using The Us National Covid Cohort Collaborative (N3c), Asif Rahman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The enduring impact of COVID-19 extends beyond acute illness, with potential long-term psychiatric consequences raising significant concern among healthcare professionals and researchers alike. Emerging evidence suggests a multifaceted relationship between COVID-19 and the development of different psychiatric illnesses like Schizophrenia Spectrum and Psychotic Disorders (SSPD), Depression, Bipolar disorder, Personality disorder, Trauma, and a range of other mental health conditions. Considering these emerging connections, our study endeavors to rigorously assess the associations between COVID-19 and various psychiatric illnesses while simultaneously employing machine learning techniques to predict the development of new psychiatric disorders in individuals affected by the virus. Leveraging the extensive …

Breaking The Silence: International Students’ Communication About Sexual Assault And Opportunities For Improved Prevention Messaging, Samaha Ghani

Graduate Theses, Dissertations, and Problem Reports (ETD)

The beginning of college campus life is one of the most exciting phases of student life. However, for some students, it might pose a threat in the form of sexual violence. In the United States, sexual violence is highly pervasive, placing college students at a greater risk of victimization. Currently, there is a lack of data on sexual crimes within the international student population on U.S. college campuses due to cultural and language barriers. As such, the purpose of this study is to explore international students’ communication needs around the topic of sexual consent and sexual violence. This vulnerable and …

Iron Coated Sand: A Sustainable Substrate Amendment For Nutrient Management And Growth Of Floriculture Crops, Savannah Mead

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid mine drainage and phosphorus pollution are two of the most pervasive forms of water contamination in Appalachia and globally. The horticultural industry is a contributor to nutrient pollution via leaching from greenhouses, necessitating the adoption of more sustainable practices for container production in controlled environments. In this thesis, an acid mine drainage-based iron-coated sand product was assessed on its effectiveness for phosphate adsorption when used as a sustainable substrate amendment for soilless container production of flowering ornamentals. The first study centered around the amount of iron-coated sand to use for production of petunias, pansies, and chrysanthemums. Iron-coated sand at …

Bystander Interventions For Sexual Assault Prevention Among Students In Greek Life, Caterina Defazio

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Bystander-based sexual violence (SV) prevention programs are critical on college campuses to meet federal Title IX requirements and have proven effective in reducing violence. Greek-affiliated students (members of fraternities and sororities) are at a higher risk for sexual assault than their peers, yet current training programs often do not address their specific needs. This dissertation comprises three interconnected studies that collectively aim to improve bystander intervention strategies for this high-risk group. Aim 1 - Scoping Review: A scoping review was conducted to identify and describe bystander intervention programs delivered to Greek-affiliated students in the US and Canada. An eight-database …

Cellular Level Changes Induced In Cancer Cells Due To Microgravity, Sai Deepika Reddy Yaram

Graduate Theses, Dissertations, and Problem Reports (ETD)

The increase in human activity in space has revealed several kinds of physiological challenges brought on by the absence of Earth's gravitational pull. NASA observed that during spaceflight, astronauts experience a 1-1.5% monthly loss in the mineral density of weight-bearing bones, with rehabilitation efforts on Earth often proving ineffective. This issue extends to muscles, the neuro vestibular system, the cardiovascular system, and vision. Cancer has emerged as a significant cause of mortality among astronauts, with studies up to 2017 showing that 41.3% of natural cause deaths among U.S. astronauts were due to cancer, primarily due to space radiation exposure. Cancer …

Effects Of Dietary Supplementation Of A Blend Of Saccharomyces Cerevisiae, Multiple Live Probiotic Bacteria, And Their Fermentation Products On Performance, Health, And Rumen Bacterial Community Of Newly Weaned Beef Steers During A 56-D Receiving Period, Emily Treon

Graduate Theses, Dissertations, and Problem Reports (ETD)

We examined the effects of a blend of Saccharomyces cerevisiae, multiple live probiotic bacteria and their fermentation products on performance, health, and the ruminal bacterial community of newly weaned beef steers during a 56-day receiving period. Forty newly weaned Angus crossbred steers (221 ± 25.6 kg BW; 180 ± 17 d of age) were stratified by body weight into four pens (10 steers per pen) such that each pen had a similar average body weight at the beginning of the experiment. The pens were randomly assigned to receive a corn silage basal diet (CON; n = 20) or the …

Associations Among Psychosocial Factors, Health-Related Quality Of Life, And Adherence In Pediatric Inflammatory Bowel Disease, Cecelia I. Nelson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Inflammatory Bowel Disease (IBD) is a prevalent disorder among youth in the United States. IBD is one of the leading causes of chronic pain in adolescence and symptoms can lead to reduced quality of life, social embarrassment, and lower self-esteem. Adherence to medical regimens for IBD is incredibly important to prevent morbidity, mortality, and impaired health-related quality of life (HRQOL). However, adolescents struggle generally with adherence and the regimen for IBD can be complicated and include aversive side effects. Psychological factors, such as depressive symptoms, are key factors associated with non-adherence. Nevertheless, gut-specific anxiety (GSA) is a psychological factor that …

A Randomized Controlled Trial Of Group Telehealth Workshops Based On Parent-Child Interaction Therapy For Caregivers Of Children With Autism Spectrum Disorder, Erinn J. Victory

Graduate Theses, Dissertations, and Problem Reports (ETD)

Autism spectrum disorder (ASD) is a neurodevelopmental disorder associated with various symptoms that interfere with functioning requiring intensive early intervention; however, many families experience barriers to accessing behavioral services due to provider shortages, cost of treatment, and lack of transportation. Parent-Child Interaction Therapy (PCIT) has demonstrated effectiveness as a supplemental treatment for disruptive behaviors in children on the autism spectrum. The current study aims to determine the efficacy of a 2-day group telehealth parent-only workshop based on PCIT for improving caregivers’ use of positive parenting skills, child disruptive behavior, compliance to caregiver commands, and child psychosocial functioning. Thirty-three caregivers and …

A Critical Event Narrative Analysis Of African Women Immigrant Entrepreneurs In The United States: Impacts Before, During, And After The Covid-19 Pandemic, Damilola T. Fasinu

Graduate Theses, Dissertations, and Problem Reports (ETD)

This doctoral research presents a qualitative critical event narrative study focused on African Women Immigrant Entrepreneurs (AWIE) in the United States during the COVID-19 pandemic. The study aims to explore the unique challenges, coping mechanisms, and resilience strategies of AWIE by examining their experiences before, during, and after the pandemic. The research is driven by three guiding questions: (1) How do AWIE describe their experience before the pandemic? (2) During the pandemic? (3) After the pandemic? This inquiry provides a comprehensive narrative, capturing the challenges and successes experienced in the pre-pandemic environment, documenting the immediate impacts of the pandemic on …

Success Beyond Access: Examining Institutional Barriers To Persistence And The Supports Needed By Nontraditional Students Participating In A Tuition-Free Community College Program, Amanda K. Roeher

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research study examined the experiences of nontraditional community college students attending through a tuition-free grant. The intent of the study was to explore the student identified barriers to degree persistence and the student support services nontraditional students recognize as being beneficial towards their academic journey. This qualitative case study was guided by three research questions and utilized Bean and Metzner’s (1985) Nontraditional Student Attrition Model as a guide for study design and analysis. Data collection consisted of student interviews and institutional document collection. Data analysis explored themes related to nontraditional student motivations for attendance, persistence factors, and student support …

Improving Caregiver Burden For Professional Caregivers Of Dementia Residents, Austin Anderson

Nursing

This practice improvement project examines the burden on professional caregivers of persons with dementia (PWD), aiming to enhance their well-being. With dementia significantly impacting cognitive functions, caregivers face heightened levels of stress, depressive symptoms, and physical health issues, more so than those caring for individuals with other conditions. Utilizing Roy's Adaptation Model (RAM) and the Theory of Caregiver Stress, the dissertation proposes comprehensive strategies to understand and alleviate the challenges faced by caregivers. The core of this practice improvement project aims to enhance the well-being of formal caregivers by implementing a comprehensive training session. This session was designed to equip …

The Impact Of Normal Growth On The Ap Relationships Of The Jaws And Incisors To The Forehead: A Serial Cephalometric Analysis Of Untreated Individuals, Adam Joseph Rudmann Dr

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

The Impact of Normal Growth on the AP Relationships of the Jaws and Incisors to the Forehead: A Serial Cephalometric Analysis of Untreated Individuals

Adam Rudmann D.D.S. M.S.

Background and Objectives

Growth of the craniofacial complex is a critical factor to consider when treatment planning an orthodontic case. The ability to predict how the forehead and jaws will change over time depends on the ability to find predictable landmarks either radiographically or clinically, and use them to gauge the relative growth of various facial structures. This data can then be integrated with other diagnostic information such as occlusion and …

E-Cigarette Aerosol’S Effect On The Force Degradation Of Elastomeric Chain: An In Vitro Study, Joshua D. Lokant

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

E-Cigarette Aerosol’s Effect on the Force Degradation of Elastomeric Chain: An in Vitro Study

Joshua Lokant D.D.S.

Background and Objectives

Orthodontic elastomeric chain is used in orthodontic treatment for various applications such as closing spaces, aligning teeth, correcting midlines, promoting even spaces, and retracting teeth. Ideally, this force is light, continuous, and maintains a constant force between orthodontic appointments. There are many companies that manufacture polyurethane power chain, and their proprietary formulas give rise to different force levels and constancy of force. When the power chain is in the mouth, it is subject to food, liquids, temperature changes, pH …

Anti-Listeria Of H2o2-Peroxyacetic Acid Anti-Listeria Efficacy Of A Hydrogen Peroxide-Peroxyacetic Acid Mixer In Bacterial Buffered Solution And On Bell Peppers, Peighton Denae Foster

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study aims to 1) evaluate the efficacy of H₂O₂-peroxyacetic acid (PAA) mixer to inactivate Listeria monocytogenes in 0.1% buffered-peptone-water; 2) evaluate the reduction and mitigation of cross-contamination on peppers. For the study-1, aliquots of 1.0-ml mixer solutions (0.25, 0.50 and 0.84%) were added to the first 3-well of 8-strip-deep-well microplates. Then, the 0.1 ml of 10-fold dilution the Nalidixic-acid-resistant (NaL) L. monocytogenes was added and mixed immediately with a multichannel pipette. After exposure for 0, 5, 15, 30, and up to 120-s, 1 ml of 2×D/E neutralized solution was added to terminate the reaction followed …

The Perceived Effects Of Peer-Assisted Learning In A Collegiate Instrumental Pedagogy Classroom, Hannah Wagoner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Peer-assisted learning (PAL) is an approach that teachers can use in the classroom to increase students’ ownership of their learning. Besides increased autonomy, PAL can provide several other academic and social benefits, such as increased achievement and fostering a sense of community. This study aimed to examine the effects of PAL on college students’ performance on a secondary instrument. Five students in a collegiate instrumental pedagogy course experienced PAL activities in class while learning secondary instruments. After a statistical and content analysis of all the data, results showed that 1) all but one of the participants improved in their performance …

Isabella: An Eternal Badass An Actor’S Exploration On Healing, Forgiveness, And Learning To Embrace The Messiness Through Isabella In William Shakespeare’S Measure For Measure, Shelby E. Garrett

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis not only delves into the acting methods used to portray Shakespeare's Isabella in West Virginia University's spring 2023 production of Measure for Measure, but also examines the innovative approach of interpreting the character through the perspective of an older actor. It will examine acting techniques such as Meisner, Fitzmaurice Voicework®, yoga, and chakra work, detailing their influence on character development. From audition to performance, this document will track my journey, capturing personal insights through journal entries, sound bites, and images that helped in shaping a more mature Isabella. Additionally, it explores how actors navigate personal challenges during …

Rural Student Postsecondary Decision-Making: Navigating Narratives Of The Purpose And Value Of Higher Education, Ashley Renee Leggett-Bradley

Graduate Theses, Dissertations, and Problem Reports (ETD)

The narrative surrounding the purpose and place of higher education has shifted dramatically over the past few decades, despite the persistent perceptions of the necessity of an educated society. With these shifts and conflicting narratives comes the question of how students are influenced by these narratives as they make their postsecondary decisions. This study seeks to illuminate the unique way that rural students receive, understand, and are influenced by these potentially conflicting narratives through a qualitative multi-site case study. All three sites are located within the North Central Appalachian portion of the state of West Virginia, and encompass a range …

Evaluating Land Cover Change And Opportunities For Bioenergy Crop Development On Surface Mine Sites In West Virginia, U.S.A., Kenzie D. Kohrs

Graduate Theses, Dissertations, and Problem Reports (ETD)

Surface mining can impact land cover, forests, and water quality. Current reclamation strategies include revegetation with herbaceous species due to the ease and speed of cover establishment. Herbaceous and woody biomass crops have been used in various studies to reclaim surface mines and act as an alternative to nonrenewable energy sources. The objectives of this study were to quantify the state of vegetation growth on former surface mines in West Virginia over a 9-year period and identify suitable acreage for bioenergy production. During 2011 to 2020, we found that over 40,000 acres had been converted to forest and 40,000 acres …

Utilizing Suas-Based Remote Sensing For Sustainable Outdoor Recreational Trail Design And Monitoring, Isaac C. Kinder

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study utilizes sUAS-based remote sensing and hydrologic models to analyze and predict locations susceptible to water-based trail erosion. Erosion is frequently cited as the most significant environmental impact of trails and often requires costly design and management considerations. A professionally designed trail totaling 4 km in length was segmented based on presence or absence of water-based erosion for analyses and then flown with sUAS technology. Three Logistic regression (LR) models were generated utilizing several hydrologic terrain models of varying resolutions to determine the effects of spatial resolution on the models’ predictive accuracies. Receiver operator characteristics, kappa, and overall accuracy …

Essays On Urban Transportation And Health Economics, Alexander P. Tsiukes

Graduate Theses, Dissertations, and Problem Reports (ETD)

The first chapter analyzes how Premier League games in London impact demand for the city’s bike-sharing system. Bike-sharing systems affect mode choice for within-city transportation. Using bike rental data from Transport for London, this chapter exploits the plausibly-exogenous timing of Premier League games to identify how proximity to stadiums affects demand for London’s bike-sharing system. Results indicate games at Stamford Bridge (stadium) cause a 24.36% increase in the number of bike trips arriving within 0.5km of the stadium, with effects diminishing as distance increases. In contrast, games at London Stadium decrease nearby bike-sharing use and may actually crowd out would-be …

Development And Application Of Functional Immune Assays To Understand Disease In Wild Smallmouth Bass Populations, Cheyenne R. Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over recent decades, the scientific community has observed a disconcerting trend – a decline in the health and stability of smallmouth bass (Micropterus dolomieu) populations across the Chesapeake Bay watershed with growing economic and ecological implications. Disease, declines, and death have been observed in multiple locations, particularly in the Susquehanna and Potomac drainages with the adults disproportionately affected in the Potomac River basin and young-of-year in the Susquehanna River basin. There has not been a single or consistent cause to the declines or mortality events. More likely, it is believed immunosuppression relating to a complex mixture of stressors has been …

Encouraging Equity Through Rural Elementary Professional Learning, Jana K. Stone

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study employed design-based research to explore the efficacy of professional learning materials and approaches in developing social justice advocacy with rural elementary teachers in Appalachia. Bronfenbrenner’s (2005) bioecological theory and Boler’s (1999) pedagogy of discomfort formed the theoretical framework for the design, implementation, and analysis. Mid-way through the study, Allport’s (1954) contact hypothesis was included to inform the revision of intervention approaches. Four elementary teachers attended seven in-person informational discussion meetings over five months. During this time, these teachers also read two books and completed journal responses and surveys. The qualitative data was analyzed using Braun and Clark’s (2006) …

Impacts Of Community Food Outreach Programs On Low-Income Families: St. Ursula’S Food Outreach Program In Pursglove Monongalia, West Virginia, United States As A Case Study, Oluwafunke Abeleje

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Impacts Of Community Food Outreach Programs on Low-Income Families: St. Ursula’s Food Outreach Program in Pursglove Monongalia, West Virginia as A Case Study

Abeleje Oluwafunke Rebecca

According to previous research studies as stated by Fatka (2023), in 2014, 14% of households in the United States had levels of food insecurity. Also, as noted by Pitts, 2020, food insecurity rates rose across the board in the United States during the early COVID-19 epidemic and tripled among families with children. Compared to other industrialized countries, the United States had very high rates of food insecurity before the outbreak (Poblacion et al., …

Immune Outcomes Affected By Selection For Parasite Resistance In Katahdin Sheep, Kelsey Lynne Bentley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Our understanding of the complex connection between selection for parasite resistance and the overall health outcomes in livestock is unclear. This study investigates the impact of post-weaning fecal egg count (PFEC) estimated breeding values (EBV) on health outcomes and immune responses in Katahdin sheep. Selection for PFEC, favoring Low-PFEC lambs, not only affects survivability to weaning but also suggests broader implications for immunity beyond parasite resistance. The effect of PFEC EBV on antibody production in response to vaccination against Clostridium perfringens type C&D and tetanus toxoid (CD&T) revealed a stepwise decrease in immunoglobin (IgG) from Low-PFEC to High-PFEC groups upon …

Assessing The Impact Of Olive Oil And Flaxseed Oil Supplementation On Hypertension And Inflammation In Healthy And At-Risk Adults, Tara Bess Mcnabb

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Supplementation of olive oil and flaxseed oil and their impacts on hypertension and inflammation have been reported for healthy and hypertensive adults. However, a comprehensive systematic literature review and meta-analysis on the preventive benefit of plant oil supplementation in healthy adults and adults at risk of inflammation inducing hypertension is lacking.

Objective: to evaluate human clinical trials assessing the impact of olive oil and flaxseed oil capsules on systolic blood pressure (SBP) and diastolic blood pressure (DBP) and select inflammatory markers in non-hypertensive and at-risk adults of hypertension.

Data sources: Four databases were searched in October 2023 …

The Sex Chromosome Complement And Gut Microbiome Act Independently And Collaboratively To Impart Sex Dimorphisms In Humoral Immunity, Carly J. Amato-Menker

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sex dimorphisms in immunity are well-documented and have significant clinical implications. While the immunomodulatory effects of sex hormones have been extensively documented, recent recognition of the nuanced contributions of the sex chromosome complement (XX vs. XY) and the gut microbiome to immune sex dimorphisms underscores the need for a more comprehensive understanding. Within this context, the work herein focuses on unraveling the individual and collaborative impacts of the sex chromosome complement and gut microbiota on humoral immune activation. We assessed humoral immune responses to immunization with heat-killed Streptococcus pneumoniae (HKSP) in Four Core Genotype (FCG) mice and found elevated IgM-secreting …

A Test Of The Extended Theoretical Model Of Communal Coping Among Graduate Students: Investigating The Influence Of Communal Coping On Graduate Students’ Psychological Well-Being, Rebekah M. Chiasson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this dissertation was to test the extended theoretical model of communal coping (T. Afifi et al., 2020) in a graduate student sample by exploring predictors and outcomes of communal coping processes among 554 graduate students. The extended theoretical model of communal coping specifies that communal coping occurs when individuals within a community—such as graduate students within an academic program—perceive stressors as shared and are willing to take joint action to overcome those stressors. Results of this dissertation provided evidence that graduate students’ academic stress and the severity of individual academic stressors negatively impacted their psychological well-being. The …

Implementing Unmanned Aerial Vehicles To Collect Human Gait Data At Distance And Altitude For Identification And Re-Identification, Donn E. Bartram

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gait patterns are a class of biometric information pertaining to the way a person moves and poses. Gait information is unique to each person and can be used to identify and reidentify people. Historically, this task has been achieved through the use of multiple ground-based imaging sensors. However, as Unmanned Aerial Vehicles (UAVs) advance, they present the opportunity to evolve the process of persons identification and re-identification. Collecting human gait data using UAVs at distances ranging from 20m to 500m and altitudes ranging from 0m to 120m is a challenging task. The current biometric data collection methods, primarily designed for …