Digital Commons Network^™

Terminally Differentiated Effector Memory T Cells Associate With Cognitive And Ad-Related Biomarkers In An Aging-Based Community Cohort, Edric Winford, Jenny Lutshumba, Barbara J. Martin, Donna M. Wilcock, Gregory A. Jicha, Barbara S. Nikolajczyk, Ann M. Stowe, Adam D. Bachstetter

Markey Cancer Center Faculty Publications

Background and purpose: The immune response changes during aging and the progression of Alzheimer’s disease (AD) and related dementia (ADRD). Terminally differentiated effector memory T cells (called TEMRA) are important during aging and AD due to their cytotoxic phenotype and association with cognitive decline. However, it is not clear if the changes seen in TEMRAs are specific to AD-related cognitive decline specifically or are more generally correlated with cognitive decline. This study aimed to examine whether TEMRAs are associated with cognition and plasma biomarkers of AD, neurodegeneration, and neuroinflammation in a community-based cohort of older adults.

Methods: Study participants from …

Cooperative Nucleic Acid Binding By Poly Adp‑Ribose Polymerase 1, Manana Melikishvili, Michael G. Fried, Yvonne N. Fondufe‑Mittendorf

Markey Cancer Center Faculty Publications

Poly (ADP)‑ribose polymerase 1 (PARP1) is an abundant nuclear protein well‑known for its role in DNA repair yet also participates in DNA replication, transcription, and co‑transcriptional splicing, where DNA is undamaged. Thus, binding to undamaged regions in DNA and RNA is likely a part of PARP1’s normal repertoire. Here we describe analyses of PARP1 binding to two short single‑stranded DNAs, a single‑stranded RNA, and a double stranded DNA. The investigations involved comparing the wild‑ type (WT) full‑length enzyme with mutants lacking the catalytic domain (∆CAT) or zinc fingers 1 and 2 (∆Zn1∆Zn2). All three protein types exhibited monomeric characteristics in …

Non-Transcriptional Irf7 Interacts With Nf-Κb To Inhibit Viral Inflammation, Shumin Fan, Sonam Popli, Sukanya Chakravarty, Ritu Chakravarty, Saurabh Chattopadhyay

Markey Cancer Center Faculty Publications

Interferon (IFN) regulatory factors (IRF) are key transcrip- tion factors in cellular antiviral responses. IRF7, a virus- inducible IRF, expressed primarily in myeloid cells, is required for transcriptional induction of interferon α and antiviral genes. IRF7 is activated by virus-induced phosphory- lation in the cytoplasm, leading to its translocation to the nu- cleus for transcriptional activity. Here, we revealed a nontranscriptional activity of IRF7 contributing to its antiviral functions. IRF7 interacted with the pro-inflammatory tran- scription factor NF-κB-p65 and inhibited the induction of in- flammatory target genes. Using knockdown, knockout, and overexpression strategies, we demonstrated that IRF7 inhibited NF-κB–dependent inflammatory …

Foxs1 Is Increased In Liver Fibrosis And Regulates Tgfβ Responsiveness And Proliferation Pathways In Human Hepatic Stellate Cells, Evelyn A. Bates, Zachary A. Kipp, Wang-Hsin Lee, Genesee J. Martinez, Lauren Weaver, Kathryn N. Becker, Sally Pauss, Justin F. Creeden, Garrett B. Anspach, Robert N. Helsley, Mei Xu, Maria E. C. Bruno, Marlene E. Starr, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Liver fibrosis commences with liver injury stimulating transforming growth factor beta (TGFβ) activation of hepatic stellate cells (HSCs), causing scarring and irreversible damage. TGFβ induces expression of the transcription factor Forkhead box S1 (FOXS1) in hepatocytes and may have a role in the pathogenesis of hepatocellular carcinoma (HCC). To date, no studies have determined how it affects HSCs. We analyzed human livers with cirrhosis, HCC, and a murine fibrosis model and found that FOXS1 expression is significantly higher in fibrotic livers but not in HCC. Next, we treated human LX2 HSC cells with TGFβ to activate fibrotic pathways, and FOXS1 …

Nac1 Promotes Stemness And Regulates Myeloid-Derived Cell Status In Triple-Negative Breast Cancer, Chrispus Ngule, Ruyi Shi, Xingcong Ren, Hongyan Jia, Felix Oyelami, Dong Li, Younhee Park, Jinhwan Kim, Hami Hemati, Yi Zhang, Xiaofang Xiong, Andrew Shinkle, Nathan L. Vanderford, Sara Bachert, Binhua P. Zhou, Jianlong Wang, Jianxun Song, Xia Liu, Jin-Ming Yang

Markey Cancer Center Faculty Publications

Triple negative breast cancer (TNBC) is a particularly lethal breast cancer (BC) subtype driven by cancer stem cells (CSCs) and an immunosuppressive microenvironment. Our study reveals that nucleus accumbens associated pro- tein 1 (NAC1), a member of the BTB/POZ gene family, plays a crucial role in TNBC by maintaining tumor stemness and influencing myeloid-derived suppressor cells (MDSCs). High NAC1 expression correlates with worse TNBC prognosis. NAC1 knockdown reduced CSC markers and tumor cell proliferation, migration, and invasion. Addition- ally, NAC1 affects oncogenic pathways such as the CD44-JAK1-STAT3 axis and immunosuppressive signals (TGFβ, IL-6). Intriguingly, the impact of NAC1 on tumor …

Mir-27a-3p Regulates Intestinal Cell Proliferation And Differentiation Through Wnt/Β-Catenin Signalling, Chang Li, Yuning Zhou, Yinping Jiang, Zhijie Yin, Heidi L. Weiss, Qingding Wang, B. Mark Evers

Markey Cancer Center Faculty Publications

Intestinal stem cells differentiate into absorptive enterocytes, characterised by increased brush border enzymes such as intestinal alkaline phosphatase (IAP), making up the majority (95%) of the terminally differentiated cells in the villus. Loss of integ- rity of the intestinal epithelium plays a key role in inflammatory diseases and gastro- intestinal infection. Here, we show that the intestinal microRNA (miR)-27a-3p is an important regulator of intestinal epithelial cell proliferation and enterocyte differenti- ation. Repression of endogenous miR-27a-3p leads to increased enterocyte differen- tiation and decreased intestinal epithelial cell proliferation in mouse and human small intestinal organoids. Mechanistically, miR-27a-3p regulates intestinal cell …

Novel Endoscopic Approach For Treatment Of Malignant Tracheoesophageal Fistula (Tef): A Case Report, Haris Zia, Matthew D. Mccoy, Jamie L. Sturgill, Walid Hadid, Ashish P. Maskey

Markey Cancer Center Faculty Publications

Cardiac septal occluder (CSO) can be used endoscopically in treatment of tracheoesophageal fis- tula (TEF). We present a case of metastatic cervical cancer with direct tracheal invasion resulting in malignant TEF treated previously with radiotherapy and tracheal stenting twice. This TEF was closed by CSO - patent foramen ovale (PFO) occluder as palliative management to prevent recur- rent aspiration pneumonia, decrease morbidity and improve quality of life.

The Silent Scream, Christine Roberts Gettys

Liberal Studies (MA) Final Essays

Abstract: The Silent Scream (a memoir) is one woman’s journey across six decades, dealing with physical and mental health conditions. Using vignettes and stream-of-consciousness, the author allows the reader to share lived experiences, demonstrating the interplay between cerebral palsy and childhood trauma. She analyzes 1) language development, 2) the power of biopsychosocial factors to create, intensify, recognize, manage, and mitigate stress, 3) the power of learning to identify and meet needs, and 4) the effectiveness of healthcare delivery and services. She advocates for trauma-informed and life-long learning, ongoing counseling, and increased awareness and proposes that engagement is its own outcome. …

Interactions Between Multiple Stressors, Nanomaterials And A Pathogen, In Caenorhabditis Elegans, Jarad Cochran

Theses and Dissertations--Plant and Soil Sciences

Metal and metal oxide nanoparticles (NP), which have received interest for use in agriculture, show in vitro and in vivo toxicity at relatively low concentrations. Silver nanoparticles (Ag NP) and zinc oxide nanoparticles (ZnO NP) cause adverse effects in model soil organisms under environmentally relevant conditions. In most toxicity assessments of nanomaterials, model organisms are exposed to a single stressor. Though single stressor experiments are essential to understanding the fate and effects of released NP, in nature, organisms are affected by multiple stressors, including pathogens, which might exacerbate or mitigate negative effects of NP exposure. There is limited research on …

The Case For Waivable Employee Rights: A Contrarian View, William Corbett

Journal Articles

No abstract provided.

The Once And Future Clean Air Act: Impacts Of The Inflation Reduction Act On Epa's Regulatory Authority, Nicholas Bryner

Journal Articles

The Clean Air Act is one of Congress's greatest success stories. The major piece of legislation, passed in the context of environmental and public health crises, has driven technological change through regulation that has dramatically improved air quality even in a prolonged period of economic growth. In the context of climate change, however, despite many efforts since the 1990s, the Clean Air Act has not proven to be a successful legislative tool. Instead, the Act has faced complex obstacles in the statutory language and a trend in Supreme Court jurisprudence-leading up to and including the June 2022 decision in West …

Judicial Fidelity, Caprice L. Roberts

Journal Articles

Judicial critics abound. Some say the rule of law is dead across all three branches of government. Four are dead if you count the media as the fourth estate. All are in trouble, even if one approves of each branch’s headlines, but none of them are dead. Not yet.

Pundits and scholars see the latest term of the Supreme Court as clear evidence of partisan politics and unbridled power. They decry an upheaval of laws and norms demonstrating the dire situation across the federal judiciary. Democracy is not dead even when the Court issues opinions that overturn precedent, upends longstanding …

Local Government Regulation Of Ccs, Keith B. Hall

Journal Articles

Carbon capture and storage is being used in an increasing number of locations in the United States as a tool to address climate change, and many more projects are in the planning stages. Although government, industry, and some prominent environmental organizations support the use of CCS, some environmental organizations and many citizens who live in the vicinity of proposed CCS projects oppose CCS. This has prompted several local governments to enact ordinances that attempt to prohibit or regulate CCS. These ordinances take many forms-moratoria, zoning restrictions and setbacks, effective bans, outright bans, construction and operational restrictions, and various other types …

Influences Of Professors' Personality Traits, Teaching Styles, And Generational Affiliation On Technology Acceptance: A Mixed-Methods Exploration Of Blended Learning Readiness, Joey K. Freeman

Education Dissertations

The abrupt transition to emergency remote teaching during the COVID-19 pandemic offered minimal time to prepare, resulting in faculty reliance on prior pedagogical practices in the shift to an online instructional environment. Although the pandemic altered the learning environment in the short-term, it also offered a glimpse of how higher education institutions (HEIs) could transform their learning environments in the long-term. Though a body of literature exists on factors that influence technology acceptance, a limited number of studies have explored personality traits, teaching styles, and generational affiliation. An adapted Technology Acceptance Model considered these variables as antecedents to perceptions, attitudes, …

Real-World Pharmacological Anticoagulation And Clinical Outcomes Of Venous Thromboembolism In Adults With Sickle Cell Disease, Ming Chen

Theses and Dissertations (ETD)

Sickle cell disease (SCD) is an inherited disease characterized by sickle-shaped red blood cells that can slow or block blood flow. It affects about 100,000 people in the United States, and occurs more commonly in people of African descent. SCD is considered as a hypercoagulable state and venous thromboembolism (VTE) is a serious disease-specific complication. However, there have been limited real-world studies on VTE in SCD patients. This work aims to provide a comprehensive assessment of the risk factors and treatment of VTE in adults with SCD by using longitudinal real-world data. First, a retrospective cohort study on 30-day readmission …

Investigation Of Delta-Focused Ictal Electrical Source Imaging In Refractory Focal Epilepsy, Jared A. Rybarczyk

Theses and Dissertations--Electrical and Computer Engineering

Refractory focal epilepsy is characterized by the presence of seizures that cannot be controlled via anti-seizure medications. For patients suffering from this form of epilepsy, accurate identification of the seizure onset zone is a crucial step for many modalities of treatment. Electrical source imaging (ESI) allows for estimation of the seizure onset zone from electroencephalography. EEG feature extraction is an important step that can impact the final accuracy of source estimates. This work provides a review of 23 ictal ESI studies and proposes a delta-focused ictal ESI methodology. Our proposed delta-focused ictal ESI is implemented across 33 refractory focal epilepsy …

Nonuniform Sampling-Based Breast Cancer Classification, Santiago Posso

Theses and Dissertations--Electrical and Computer Engineering

The emergence of deep learning models and their success in visual object recognition have fueled the medical imaging community's interest in integrating these algorithms to improve medical diagnosis. However, natural images, which have been the main focus of deep learning models and mammograms, exhibit fundamental differences. First, breast tissue abnormalities are often smaller than salient objects in natural images. Second, breast images have significantly higher resolutions but are generally heavily downsampled to fit these images to deep learning models. Models that handle high-resolution mammograms require many exams and complex architectures. Additionally, spatially resizing mammograms leads to losing discriminative details essential …

High Reliability Motor-Drive Systems For Electric Aircraft Propulsion, Majid Tahmasbi Fard

Theses and Dissertations--Electrical and Computer Engineering

The transition towards electric propulsion in aircraft applications requires significant advancements in enhancing the efficiency and power density of power transmission and distribution systems. Among the critical power components in electric aircraft propulsion systems, power electronic converters hold paramount importance as they provide a pivotal interface between batteries and propulsion motors while providing a high degree of controllability and flexibility to the system. Integrating medium voltage DC (MVDC) and the emerging wide bandgap switching devices into power converters not only amplifies converter performance but also unlocks multifarious system-level benefits, including reduced power losses, weight savings, and the utilization of high-speed …

Identification En Laboratoire Des Éléments Essentiels Au Processus D’Intégration Sécuritaire De Cellules Cobotiques, Sabrina Jocelyn, Élise Ledoux, Damien Burlet-Vienney, Isabelle Berger, Isvieysys Armas Marrero, Chun Hong Law, Yuvin Chinniah, Abdallah Ben Mosbah, Ilian A. Bonev, Denys Denis, Laurent Giraud

Rapports de recherche scientifique

Les cobots sont apparus vers 2010 en industrie et les accidents sont très peu documentés. La gestion des risques en cobotique représente un réel défi. La littérature scientifique montre l’existence de divers modèles, méthodes et outils pour gérer les risques en cobotique, en mettant l’opérateur humain au cœur de l’intégration des applications collaboratives. Cependant, un autre humain clé de la mise en œuvre de ces applications est négligé la plupart du temps. Il s’agit de l’intégrateur, celui qui doit concevoir la cellule cobotique. À notre connaissance, deux études portant sur un même projet de conception d’un logiciel aidant à mettre …

Strafford County 2023 Annual Report Of The Commissioners, Treasurer, Other County Officers, And The Strafford County Delegation For The Year Ending December 31, 2023, Strafford County Representatives

Strafford County, NH Annual Reports

This is an annual report containing vital statistics for a county in the state of New Hampshire.

Msw Portfolio, Kandace Mcgee

Graduate Student Portfolios, Professional Papers, and Capstone Projects

No abstract provided.

A Winding Path Toward Purpose, Barry Maxwell

Graduate Student Portfolios, Professional Papers, and Capstone Projects

No abstract provided.

Diverse Minds, Shared Planet: Designing, Assessing, And Adapting Curricula To Engage Diverse Learners With Their Environment And Inspire Positive Change, Mallory Rose Lampe

Graduate Student Portfolios, Professional Papers, and Capstone Projects

The pieces that compose this portfolio together explore how environmental education can connect diverse learners to their environment through the integration of accessible curricula to inspire stewardship and action to take care of the planet we share.

Safe Sleep Education For Local Refugee And Immigrant Populations, Sara Jones

Posters

Provide Safe Sleep education to local refugee and immigrant populations through their resettlement agency Jewish Vocational Services (JVS) with resources available in their first language.

Himalayan Health Exchange: Kargiakh Medical Expedition, Rachel Oldfather

Posters

Medical expedition to remote Himalayan villages involving high-altitude treks, improvised clinics, and immersion in the local Buddhist culture.

Global Health Elective - Philippines, Hannah Ho

Posters

Provides an overview of experiences working in the Philippines as a pediatric resident.

Expanding The Winter Warmth, Sarah Gwazdacz

Posters

Reviews the efforts to expand collection of winter apparel for refugee families in Kansas City.

Healthcare In Under-Resourced Areas, Gabrielle Fleming, Ryan Northup, Bill Houseworth, Lori Housworth

Posters

Describes pediatric resident's experience working in the inpatient unit, ER, PICU, and NICU in Cambodia.

Brightpoint Community College: Effective Enrollment Anti-Melt Strategies, Michael J. Forder, Ismael J. Betancourt Velez, Adrian Petway, Juan Espinoza

Doctor of Education Capstones

Community colleges strive to provide affordable and accessible educational opportunities to all. Tuition revenue plays a significant role in college operating budgets and is critical for supporting academic functions. Student dropout threatens the fiscal security of community colleges while running counter to the mission of educating communities. This exploratory case study set out to understand the student melt issue due to non-payment at Brightpoint Community College taking place between enrollment and the start of classes. The research team aimed to understand how students engage with the current payment process, what are the underlying factors leading to missed payments, and what …

Combating Chronic Absenteeism: A Practical Approach To Improving Attendance In A Mid-Sized Virginia School Division, Alissa Fraser, Caroline Goddard, Brian Raska, Michael Sidebotham

Doctor of Education Capstones

Chronic absenteeism poses a significant public health challenge in the United States, impacting educational outcomes and student well-being. This research explores strategies to address chronic absenteeism within Client Public Schools (CPS) before and after the COVID-19 pandemic (2015-present). Using a mixed-methods approach, the study integrates a literature review, quantitative analysis of CPS attendance data, and qualitative insights from focus groups and interviews. Findings reveal disparities among student groups, increased absenteeism post-pandemic, and multifaceted barriers to attendance, including school-related challenges and non-school-related factors. The study underscores the necessity for a unified strategy to track attendance data, consistent implementation of the Multi-Tiered …