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Conformational Investigation Of Biopolymers Through Molecular Dynamics (Md) Simulations And In-Droplet Hdx-Ms Utilizing Cvssi, Samira Hajian Foroushani

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the last two decades, mass spectrometry (MS) has received significant interest in biological study platforms, especially in the areas of proteomics and metabolomics. With the development of soft ionization methods like electrospray ionization (ESI), mass spectrometry has emerged as a potent method for directly characterizing proteins in their native state. The recent invention of capillary vibrating sharp-edge spray ionization has opened new avenues for the study of biopolymer native structure including the usage of droplet charge tuning and in-droplet hydrogen-deuterium exchange (HDX) labeling. With this new technique comes the opportunity to study biopolymer ion structure preservation with molecular dynamics …

Pathways To Health: Linking Accessible Green Space To Improved Health And Well‐Being In Rural Appalachia., Udday Shankur Datta

Graduate Theses, Dissertations, and Problem Reports (ETD)

Green spaces are essential for our physical health. The quality of its green space often determines the health of a community. The presence of green space in our lives is associated with reduced stress levels, better sleep, and increased feelings of safety. Lack of access to green space can lead to many health issues, such as high blood pressure, diabetes, and cardiovascular diseases, and has been linked to obesity and physical inactivity.

The Appalachian Regional Commission (ARC) defines Appalachia as a region covering 420 counties across 13 states in the eastern United States. A large part of this region is …

Celluloid Shakespeare: An Exploration Of The Relationship Between Film And Theatre Through Edgar In Shakespeare’S King Lear, Liam Michael Holton

Graduate Theses, Dissertations, and Problem Reports (ETD)

This document explores the actor‘s process and performance of Edgar in the 2021 WVU/WVPB production of William Shakespeare‘s King Lear. This production was directed by Professor Jerry McGonigle and was filmed during the height of the Covid-19 pandemic in partnership with West Virginia Public Broadcasting. This experimental project would go on to receive national distribution by the National Educational Television Association (NETA) and was also in consideration for a NETA award, in addition to receiving an Ohio Valley regional Emmy Award nomination as a finalist in the category of Arts/Entertainment – Short Form or Long Form Content. The following text …

The Role Of Uric Acid In Metabolic Abnormalities Of Obesity, Andrew P. Giromini

Graduate Theses, Dissertations, and Problem Reports (ETD)

Uric acid (UA) is the single most tightly associated metabolite for obesity and type 2 diabetes. While the field varies on whether UA is causative or correlative of the metabolic dysfunction allied to obesity, these studies use different diets and animal models to make these claims. It is the goal of this dissertation to compare different diets commonly used in this research to delve deeper into the data and determine which diets induce elevated circulating UA levels as well as which diets also induce metabolic dysfunction. Early chapters of this dissertation examine the comparison of high fructose consumption, both in …

Synaptic Zinc Transporters Support Normal Synapse Structure And Function In Mouse Auditory Cortex, Abbey Manning

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many regions of the brain are highly enriched with free zinc inside glutamatergic vesicles at presynaptic terminals. At these synapses, zinc transporter 3 (ZnT3) moves zinc into vesicles where it is co-released with glutamate. Alterations in ZnT3 are implicated in multiple neurodevelopmental disorders, and ZnT3 knock-out (KO) mice – which lack synaptic zinc – show behavioral deficits associated with autism spectrum disorder and schizophrenia. Shank3 is a synaptic scaffolding protein that assists in tethering and organizing structural proteins and glutamatergic receptors in the postsynaptic density of excitatory synapses. The localization of Shank3 at excitatory synapses and the formation of stable …

Overturning Race-Conscious Admissions: Analyzing The History Of Higher Education Affirmative Acton Legal Discourse And Preserving Whiteness, Hiba A. Qari

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although researchers have investigated Affirmative Action, few have attempted to understand and explain the language mechanisms shaping public opinion and policy. The purpose of this study was to explore the historical context of higher education Affirmative Action legal discourse. This study employed Critical Discourse Analysis and Critical Race Theory to analyze how language and race, as social constructs, maintain White dominance in law and higher education. The thematic concepts included power, White normativity, and diversity rhetoric. This project explored the historical evolution of the U.S. Supreme Court’s decisions on Affirmative Action policy in higher education. The study also investigated the …

An Inhibitory Recurrent Network In The Olfactory System, Farzaan Salman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neuromodulatory circuits are a necessary component of neural flexibility as they are able to act differentially depending on the receptors expressed by their downstream targets. Identifying the synaptic partners of modulatory neurons and determining the neurotransmitter and receptor content of these partners is necessary to identify the rules governing neuromodulatory circuits. In this study, I characterized the circuit of the contralaterally projecting, serotonin-immunoreactive deutocerebral neurons (CSDns), the sole synaptic source of the neuromodulator serotonin in the olfactory circuit of Drosophila melanogaster, by identifying the strongest synaptic partners of the CSDns, and the expression of genes encoding neurotransmitters and receptors of …

Coal As A Resource For Rare Earth Elements In West Virginia, Rachel Elizabeth Yesenchak

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although rare earth elements and yttrium (REY) are essential for manufacturing technologies vital to economic and national security, the U.S. is heavily reliant on foreign imports of these critical metals. There is significant interest in identifying and developing unconventional REY resources including coal and coal-byproducts to help secure domestic supplies of these elements. Appalachian Basin coals and byproducts are particularly enriched in REY. Coal-producing states within the basin, including West Virginia, can utilize existing infrastructure and legacy coal mining wastes to transition into the production of REY. However, in order to develop these resources, it is critical to understand the …

The Search, The Localization, And The Characterization: Fast Radio Transients, Reshma Anna Thomas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fast radio transients are energetic bursts in centimeter or meter wave-lengths, lasting only milliseconds, and originating from both Galactic and extra-Galactic sources. In our Galaxy, these transients primarily originate from pulsars, which are rotating neutron stars. The more energetic extra-galactic transients, known as fast radio bursts (FRBs), have unknown origins. This thesis explores various techniques, including single dish and interferometric observations, to detect and accurately localize fast radio transients. It also investigates how the propagation effects of radio waves can reveal characteristics of the local environment and potential FRB sources. Furthermore, it demonstrates how FRBs can be used to study …

Performance And Field Testing Of Geotextiles For Dewatering Rare Earth Element Hydraulic Preconcentrate From Hardrock Amd, Ian R. Bird

Graduate Theses, Dissertations, and Problem Reports (ETD)

As part of ongoing efforts to develop a domestic Rare Earth Elements (REE) supply chain the Hydraulic Preconcentrate (HPC) produced at the Horseshoe bend treatment plant (HsB) was manufactured and evaluated as a potential source for feedstock. After the existing clarifiers on the site were flipped to the desired pH set points, HPC was manufactured and pumped to 24 geobags. The geobags were then passively dewatered to increase the total solids percentage. The geobags evaluated were comprised of: Woven geotextiles, composite (woven and non- woven), and geobags augmented with Capillary Channel Fiber (CCF). Column Filtration Tests (CFTs) were performed onsite …

A Grounded Theory Investigation Of Basic Psychological Needs Theory As A Framework For Effective Mental Health Referrals In Collegiate Sport, Luna Ugrenović

Graduate Theses, Dissertations, and Problem Reports (ETD)

Using a grounded theory approach, this study explored collegiate athletes’ mental health referral experiences and identified mechanisms explaining those experiences based upon perceptions of psychological need fulfillment and thwarting according to Basic Psychological Needs Theory (BPNT). Interviews were conducted with a purposive, maximum variation sample of 14 current and former collegiate athletes who were referred to mental health services by support personnel or other athletes. Analyses yielded three overarching theoretical categories/properties: pre-referral factors, proactive and reactive referral conversations, and referral outcomes. Referral is often understood as an isolated conversation in which athletes are provided mental health resources in response to …

Efficient Classification Of Very High Resolution Images, Mohammad I. Nouyed

Graduate Theses, Dissertations, and Problem Reports (ETD)

In recent decades, deep learning approaches have shown significant improvement in various image understanding tasks. However, analysis of high-resolution images remains a major challenge. In this work, we address the challenge of very high-resolution histopathological image (VHRHI) classification using a new information-theoretic discriminative patch selection approach. We show results on a high-resolution image dataset, namely, gigapixel whole slide tissue images for cancer tumors. Then we address how to efficiently classify challenging histopathology images, such as gigapixel whole-slide images for cancer diagnostics with image-level annotation. These ``weak labels'' are applied throughout the image but describe tumor regions of variable sizes and …

Foraging Economies: A Market Based Methodology For Robotic Swarm Foraging, John A. Little

Graduate Theses, Dissertations, and Problem Reports (ETD)

Swarm robotics involves coordinating large groups of autonomous agents to accomplish complex tasks through decentralized, adaptive behaviors, providing a robust and scalable approach suited to dynamic and unpredictable environments. While traditional swarm models frequently draw inspiration from biological systems such as ant colonies or bee foraging, other approaches use techniques from physics, control theory, and economics to achieve effective coordination. This study distinguishes itself by applying economic principles—specifically, market-driven mechanisms like auctions, utility functions based on opportunity cost, and supply-demand dynamics based on fluctuating resource values at a central base—to improve task allocation within a swarm foraging context. This approach …

Benchmarking The Global Warming Potential Of Asphalt Pavements In West Virginia, Md Reasad Samrat

Graduate Theses, Dissertations, and Problem Reports (ETD)

With the rising focus on sustainable infrastructure, interest in environmentally friendly paving practices has grown significantly. This thesis investigates the environmental impacts of asphalt mixtures in West Virginia from a sustainable asphalt mix design perspective, with a particular emphasis on the utilization of Life Cycle Assessment (LCA). The research aligns with the U.S. Federal Highway Administration (FHWA) Climate Challenge, which aims to encourage state Departments of Transportation (DOTs) to implement low-carbon materials and consider the ecological effects of their paving activities. This study establishes the initial benchmark values for greenhouse gas emissions associated with asphalt pavements in West Virginia. The …

It's Written All Over Your Face: A Cultural And Gender History Of The Face In The Middle Ages, 1170-1500, Lacey N. Bonar Hull

Graduate Theses, Dissertations, and Problem Reports (ETD)

At a time of vast social and cultural shifts, people living in western Europe from the late twelfth to the late fifteenth centuries developed and employed methods to “read” the faces of others. This practice promoted the interpretation of inner qualities from external appearances, often used to communicate adherence to or deviation from boundaries of gendered behaviors. My project explores medieval understandings concerning this particular feature of the human body, analyzing discourses concerning the face as a site of symbolism for inner character. While scholars have studied understandings of the self and the “Other” during this period, the role of …

A Multimodal Physical Fatigue Assessment Method Using A Biomarker And Accelerometer-Embedded Wearable Wristband, Md Hadisur Rahman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Physically demanding tasks pose significant challenges to worker health, safety, and productivity across various industrial sectors in the United States. The construction industry is particularly affected due to the labor-intensive nature of its tasks and harsh environmental conditions. The industry suffers from unsatisfactory occupational health and safety records, with physical fatigue being a major contributor. Physical fatigue not only affects individual well-being and workplace safety but also influences productivity and the United States’ economy. Given the high incidence of injuries and accidents in the construction industry, assessing physical fatigue has become critical for improving worker safety and productivity. To address …

Machine Learning Based Intrusion Detection Framework For Can Bus Vulnerabilities In Modern Vehicles, Obinna C. Agbo

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Controller Area Network (CAN) bus is a crucial communication backbone in modern vehicles, connecting various Electronic Control Units (ECUs). However, inherent design weaknesses such as the lack of encryption and authentication make CAN networks vulnerable to cyber-attacks, including spoofing, Denial of Service (DoS), and fuzzing attacks. This thesis thoroughly evaluates these vulnerabilities and the limitations of existing security frameworks like Message Authentication Codes (MACs) and encryption, advocating for the adoption of Intrusion Detection Systems (IDS) as a more practical solution for CAN bus security. The proposed IDS leverages advanced machine learning techniques to accurately detect intrusions, even under complex …

Effect Of Specific Data Variations On Automated Speaker Recognition, Ethan David Meighen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Speaker recognition is not a new biometric modality but there are still many obstacles in the way in order for it to become as used as fingerprint recognition, facial recognition, and iris recognition. Many real-world environmental conditions, hardware device variations, and human behavior present serious challenges to the use of opportunistic voice or speaker samples for identification purposes. Non-idealities, identified as nuisance factors, include environmental noise, input device quality, length of utterance, sample rate variation, and unscripted data are common nuisance factors that can impact speaker recognition match score performance. The impact of the nuisance factors listed above were evaluated …

Implementation Of A Targeted “Micro-Learning” Inpatient Diabetes Education Program In A Rural Hospital Setting, Melissa Lynn Fannon-Wisner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction/Background: Diabetes affects a large portion of Americans and West Virginians annually. Inpatient hospitalization provides an opportunity to provide evidence-based diabetes education to patients. A lack of access to diabetes education at rural hospitals across the United States exists, creating a need for patients who require education while hospitalized. An intervention needs to be explored to bridge the gap for patients that need education when the diabetes educator is not available.

Purpose: The purpose of this Doctor of Nursing Practice (DNP) project is to create and implement a video micro-learning diabetic education series that can be utilized for diabetes self-management …

Political Scandals And The Print Media: A Content Analysis Of The Press Coverage Of The Access Hollywood Scandal, Biju Thiruthanathy Devassy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sex scandals have been a significant focus over the years in both academic research and media coverage. The Access Hollywood scandal of 2005 was not going to be an exception. Yet, it remains to be seen how the print media have reported these scandals related to high-profile politicians. In this study, the researcher investigates how the print media responded to the scandal in terms of amplifying or countering the narratives in the political discourse. To do so, 83 articles were reviewed during the first two weeks of the Access Hollywood scandal following the previous work of Larson (2016). Framing theory …

Improving Awareness And Identifying Risks For Emergence Delirium In Military Veterans, Hannah E. Pino

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Emergence delirium (ED) is an acute state of confusion during the recovery phase from anesthesia that may be life-threatening, especially in the veteran population. Military veteran status may be explored by the Have You Ever Served questionnaire that was developed by the American Academy of Nursing to identify veterans upon admission to the hospital and thus be able to better care for them during their stay. This campaign has been in effect at the hospital site of this project for nearly 10 years. The questionnaire identifies military history risk factors such as combat history or a diagnosis of post-traumatic …

Development And Application Of Functional Immune Assays To Understand Disease In Wild Smallmouth Bass Populations, Cheyenne R. Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over recent decades, the scientific community has observed a disconcerting trend – a decline in the health and stability of smallmouth bass (Micropterus dolomieu) populations across the Chesapeake Bay watershed with growing economic and ecological implications. Disease, declines, and death have been observed in multiple locations, particularly in the Susquehanna and Potomac drainages with the adults disproportionately affected in the Potomac River basin and young-of-year in the Susquehanna River basin. There has not been a single or consistent cause to the declines or mortality events. More likely, it is believed immunosuppression relating to a complex mixture of stressors has been …

A Test Of The Extended Theoretical Model Of Communal Coping Among Graduate Students: Investigating The Influence Of Communal Coping On Graduate Students’ Psychological Well-Being, Rebekah M. Chiasson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this dissertation was to test the extended theoretical model of communal coping (T. Afifi et al., 2020) in a graduate student sample by exploring predictors and outcomes of communal coping processes among 554 graduate students. The extended theoretical model of communal coping specifies that communal coping occurs when individuals within a community—such as graduate students within an academic program—perceive stressors as shared and are willing to take joint action to overcome those stressors. Results of this dissertation provided evidence that graduate students’ academic stress and the severity of individual academic stressors negatively impacted their psychological well-being. The …

Associations Between Diabetes-Specific Psychological Flexibility, Family Conflict, Parental Distress And Adherence In Adolescents With Type 1 Diabetes, Rebecca I. Laquaglia

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Type 1 diabetes (T1D) is a chronic disease that impacts the lives of millions of children and adolescents in the United States. Adherence to diabetes medical regimens is often challenging, particularly among adolescents. Based on previous research, diabetes-specific psychological flexibility, or the ability of an individual with T1D to psychologically adjust to, accept, and commit action to manage their diagnosis, may improve adherence in youth with T1D. Factors such as diabetes-specific family conflict (i.e., conflict within parent-child dyads that is specifically related to T1D management) and diabetes-specific parental distress (i.e., emotional distress experienced by a parent or caregiver as …

Immune Outcomes Affected By Selection For Parasite Resistance In Katahdin Sheep, Kelsey Lynne Bentley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Our understanding of the complex connection between selection for parasite resistance and the overall health outcomes in livestock is unclear. This study investigates the impact of post-weaning fecal egg count (PFEC) estimated breeding values (EBV) on health outcomes and immune responses in Katahdin sheep. Selection for PFEC, favoring Low-PFEC lambs, not only affects survivability to weaning but also suggests broader implications for immunity beyond parasite resistance. The effect of PFEC EBV on antibody production in response to vaccination against Clostridium perfringens type C&D and tetanus toxoid (CD&T) revealed a stepwise decrease in immunoglobin (IgG) from Low-PFEC to High-PFEC groups upon …

The Influence Of Faculty Mentorship On Graduate Student Development Through The Lens Of Self-Efficacy, Connor Lynn Ferguson

Graduate Theses, Dissertations, and Problem Reports (ETD)

This exploratory sequential mixed-methods case study explores the relationship between perceived self-efficacy and faculty mentorship with biomedical sciences graduate students. The intent of the study was to understand the extent at which faculty mentorship contributes to self-efficacy development in the population of biomedical sciences doctoral students enrolled at a mid-Atlantic R1 institution. Data collected consisted of a survey assessing perceived self-efficacy and mentoring, interviews and documents. Data analysis explored themes related to the perceived academic and professional development of the students through their responses. The research highlights the significance of the mentor-mentee relationship and influence of the mentor on the …

Investigating Birds As Dispersal Vectors Of Litylenchus Crenatae Subsp. Mccannii (Anguinidae), The Nematode Associated With Beech Leaf Disease, Spencer Rock Parkinson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Beech leaf disease (BLD) is an emerging forest pathogen primarily affecting American beech (Fagus grandifolia, Ehrh.) in North America and has been attributed to tree mortality of sapling sized trees within five to seven years of infection. Symptoms typically occur in regenerating American beech thickets sprouting from roots of trees killed by beech bark disease. Scientists first observed BLD in Ohio in 2012 and currently has spread to 15 states in the USA and one Canadian province. The nematode Litylenchus crenatae subsp. mccannii (Lcm) is highly associated with BLD symptoms, interveinal chlorosis and defoliation of leaves, and is currently …

Usage Of Peer Mentoring Workbooks: Enhancing The Transition Of College Students With Intellectual And Developmental Disabilities, Taylor Nicole Grout

Graduate Theses, Dissertations, and Problem Reports (ETD)

This doctoral capstone project assesses the impact and effectiveness of using peer mentoring workbooks as a tool to enhance the transition of college students with intellectual and developmental disabilities. A peer mentoring workbook was developed using theoretical frameworks to guide its design and identification of primary occupations, roles, and routines of college students. From here, four domains of college student life (dorm living, college social life, classroom management and participation, and community engagement and transportation) were developed to act as a framework for the workbook. Through the usage of Participatory Action Research, key stakeholders provided essential feedback that allowed for …

Limb Speed Perception Accuracy In Neurodegenerative Disease, Brandmeir Lynn Cheryl

Graduate Theses, Dissertations, and Problem Reports (ETD)

Movement is a fundamental human activity, enabling us to engage with our surroundings. Consequently, a well-operating neuromuscular system is essential for maintaining quality of life. Motion is not only integral for the basic function of daily living, but also plays a significant role in enhancing our physical, mental, and emotional health. The importance of this system becomes abundantly clear when it is compromised, such as with neurodegenerative disease.

Alzheimer’s disease (AD), followed by Parkinson’s disease (PD) are the two most common neurodegenerative diseases that profoundly affect millions of individuals worldwide (Lamptey et al., 2022). Characterized by distinct but sometimes overlapping …

From Class To Craft: An Investigation Into The Practice Of Acting Techniques Applied To Angelo In Shakespeare’S Measure For Measure, Charles Wesley Meeks

Graduate Theses, Dissertations, and Problem Reports (ETD)

This document examines the character development and performance process of Angelo in Measure for Measure by William Shakespeare. This production was directed by Professor Jerry McGonigle and performed in the mainstage season of 2022-2023 School of Theatre & Dance at West Virginia University. The ensuing text will outline the production process from the actor’s point of view including preparations, auditions, casting, rehearsals, performances, and final reflection. This text will include historical research, personal and directorial notes, textual analysis, table work and discussion, acting techniques, training, and education applied throughout the production period, and reflections of these experiences and discoveries.