Digital Commons Network^™

The Emerging Role Of Notch3 Receptor Signalling In Human Lung Diseases, Grant W Goodman, Patrick Devlin, Bryce E West, Rodney M Ritzel

Faculty, Staff and Student Publications

The recent identification of skull bone marrow as a reactive hematopoietic niche that can contribute to and direct leukocyte trafficking into the meninges and brain has transformed our view of this bone structure from a solid, protective casing to a living, dynamic tissue poised to modulate brain homeostasis and neuroinflammation. This emerging concept may be highly relevant to injuries that directly impact the skull such as in traumatic brain injury (TBI). From mild concussion to severe contusion with skull fracturing, the bone marrow response of this local myeloid cell reservoir has the potential to impact not just the acute inflammatory …

Voluntary Wheel Running Prevents Formation Of Membrane Attack Complexes And Myelin Degradation After Peripheral Nerve Injury, Suzanne M Green-Fulgham, Michael J Lacagnina, Kendal F Willcox, Jiahe Li, Michael E Harland, Adriano Polican Ciena, Igor R Correia Rocha, Jayson B Ball, Renee A Dreher, Younus A Zuberi, Joseph M Dragavon, Marucia Chacur, Steven F Maier, Linda R Watkins, Peter M Grace

Faculty, Staff and Student Publications

Regular aerobic activity is associated with a reduced risk of chronic pain in humans and rodents. Our previous studies in rodents have shown that prior voluntary wheel running can normalize redox signaling at the site of peripheral nerve injury, attenuating subsequent neuropathic pain. However, the full extent of neuroprotection offered by voluntary wheel running after peripheral nerve injury is unknown. Here, we show that six weeks of voluntary wheel running prior to chronic constriction injury (CCI) reduced the terminal complement membrane attack complex (MAC) at the sciatic nerve injury site. This was associated with increased expression of the MAC inhibitor …

Case Report: Early Acute Kidney Failure In An 11-Year-Old Boy With Dent Disease Type 1, Nicolette Murphey, Craig Authement, Paul Hillman, Samhar I Al-Akash, Kate Richardson

Faculty, Staff and Student Publications

Dent disease type 1 (Dent 1) is a rare X-linked genetic condition which impacts kidney function and is caused by pathogenic variants in CLCN5. Affected males typically develop low molecular weight proteinuria, hypercalciuria, nephrocalcinosis, nephrolithiasis, and other symptoms. Kidney failure often occurs between the third to fifth decade of life. Here, we report an 11-year-old boy with Dent 1 and a severe kidney disease phenotype. The patient presented with flank pain, nocturnal enuresis, foamy urine, and increased urinary frequency. He was found to have nephrotic-range proteinuria, without hypoalbuminemia, and a significantly decreased estimated glomerular filtration rate at presentation. Further, he …

Endoscopic Innovations In Diagnosis And Management Of Pancreatic Cancer: A Narrative Review And Future Directions, Prateek Suresh Harne, Vaishali Harne, Curtis Wray, Nirav Thosani

Faculty, Staff and Student Publications

Pancreatic cancer serves as the third leading cause of cancer-associated morbidity and mortality in the United States, with a 5-year survival rate of only 12% with an expected increase in incidence and mortality in the coming years. Pancreatic ductal adenocarcinomas constitute most pancreatic malignancies. Certain genetic syndromes, including Lynch syndrome, hereditary breast and ovarian cancer syndrome, hereditary pancreatitis, familial adenomatous polyposis, Peutz-Jeghers syndrome, familial pancreatic cancer mutation, and ataxia telangiectasia, confer a significantly higher risk. Screening for pancreatic malignancies currently targets patients with germline mutations or those with significant family history. Screening the general population is not currently viable owing …

Diet-Stimulated Marrow Adiposity Fails To Worsen Early, Age-Related Bone Loss, Cody Mcgrath, Sarah E Little-Letsinger, Gabriel M Pagnotti, Buer Sen, Zhihui Xie, Gunes Uzer, Guniz B Uzer, Xiaopeng Zong, Martin A Styner, Janet Rubin, Maya Styner

Faculty, Staff and Student Publications

Introduction: Longitudinal effect of diet-induced obesity on bone is uncertain. Prior work showed both no effect and a decrement in bone density or quality when obesity begins prior to skeletal maturity. We aimed to quantify long-term effects of obesity on bone and bone marrow adipose tissue (BMAT) in adulthood.

Methods: Skeletally mature, female C57BL/6 mice (n = 70) aged 12 weeks were randomly allocated to low-fat diet (LFD; 10% kcal fat; n = 30) or high-fat diet (HFD; 60% kcal fat; n = 30), with analyses at 12, 15, 18, and 24 weeks (n = 10/group). Tibial microarchitecture was analyzed …

Sustained Pain And Macrophage Infiltration In A Mouse Muscle Contusion Model, Ibdanelo Cortez, Caitlyn M Gaffney, Caitlin V Crelli, Eric Lee, James M Nichols, Hoang Vu Pham, Syed Mehdi, Jelena M Janjic, Andrew J Shepherd

Faculty, Staff and Student Publications

Introduction/aims: Prior studies have emphasized the role of inflammation in the response to injury and muscle regeneration, but little emphasis has been placed on characterizing the relationship between innate inflammation, pain, and functional impairment. The aim of our study was to determine the contribution of innate immunity to prolonged pain following muscle contusion.

Methods: We developed a closed-impact mouse model of muscle contusion and a macrophage-targeted near-infrared fluorescent nanoemulsion. Closed-impact contusions were delivered to the lower left limb. Pain sensitivity, gait dysfunction, and inflammation were assessed in the days and weeks post-contusion. Macrophage accumulation was imaged in vivo by injecting …

Single-Cell Rna Sequencing Analysis Identifies Acute Changes In The Tumor Microenvironment Induced By Interferon Α Gene Therapy In A Murine Bladder Cancer Model, Alexis R Steinmetz, Morgan Pierce, Alberto Martini, Come Tholomier, Ganiraju Manyam, Yan Chen, Akshay Sood, Jonathan J Duplisea, Burles A Johnson, Bogdan A Czerniak, Byron H Lee, Chinnaswamy Jagannath, Seppo Yla-Herttuala, Nigel R Parker, David J Mcconkey, Colin P Dinney, Sharada Mokkapati

Faculty, Staff and Student Publications

Introduction: Nadofaragene firadenovec (Ad-IFNα/Syn3) is now approved for BCG-unresponsive bladder cancer (BLCA). IFNα is a pleiotropic cytokine that causes direct tumor cell killing via TRAIL-mediated apoptosis, angiogenesis inhibition, and activation of the innate and adaptive immune system. We established an immunocompetent murine BLCA model to study the effects of murine adenoviral IFNα (muAd-Ifnα) gene therapy on cancer cells and the tumor microenvironment using a novel murine equivalent of Nadofaragene firadenovec (muAd-Ifnα).

Methods: Tumors were induced by instilling MB49 cells into the bladders of mice; luciferase imaging confirmed tumor development. Mice were treated with adenovirus control (Ad-Ctrl; empty vector), or muAd-Ifnα …

Meta-Analysis Of The Vmp-Like Sequences Of Lyme Disease Borrelia: Evidence For The Evolution Of An Elaborate Antigenic Variation System, Steven J Norris, Kalvis Brangulis

Faculty, Staff and Student Publications

VMP-like sequence (vls) antigenic variation systems are present in every Lyme disease Borrelia strain with complete genome sequences. The linear plasmid-encoded vls system consists of a single expression site (vlsE) and contiguous array(s) of silent cassettes that have ~90% identity with the central cassette region of the cognate vlsE gene; antigenic variation occurs through random, segmental, and unidirectional recombination of vls silent cassette sequences into the vlsE expression site. Automated annotation programs do not accurately recognize vls silent cassette sequences, so these regions are not correctly annotated in most genomic sequences. In this study, the vls …

Fa4sans-Gan: A Novel Machine Learning Generative Adversarial Network To Further Understand Ophthalmic Changes In Spaceflight Associated Neuro-Ocular Syndrome (Sans), Sharif Amit Kamran, Khondker Fariha Hossain, Joshua Ong, Ethan Waisberg, Nasif Zaman, Salah A Baker, Andrew G Lee, Alireza Tavakkoli

Faculty, Staff and Student Publications

PURPOSE: To provide an automated system for synthesizing fluorescein angiography (FA) images from color fundus photographs for averting risks associated with fluorescein dye and extend its future application to spaceflight associated neuro-ocular syndrome (SANS) detection in spaceflight where resources are limited.

DESIGN: Development and validation of a novel conditional generative adversarial network (GAN) trained on limited amount of FA and color fundus images with diabetic retinopathy and control cases.

PARTICIPANTS: Color fundus and FA paired images for unique patients were collected from a publicly available study.

METHODS: FA4SANS-GAN was trained to generate FA images from color fundus photographs using 2 …

From Mitochondria To Tumor Suppression: Acat1’S Crucial Role In Gastric Cancer, Wei He, Yanfang Li, Song-Bai Liu, Ying Chang, Shiyuan Han, Xingyu Han, Zixin Ma, Hesham M Amin, Yao-Hua Song, Jin Zhou

Faculty, Staff and Student Publications

Acetyl CoA acetyltransferase 1 (ACAT1), a mitochondrial enzyme, is mainly involved in the formation and decomposition of ketones, isoleucine, and fatty acids. Previous clinical studies showed that mutations in the ACAT1 gene lead to ketoacidosis, Notably the role of ACAT1 in human cancer' pathogenesis varies depending on cancer type, and its specific role in gastric cancer remains largely unknown. In the current study, we found that the expression of ACAT1 in primary late-stage gastric cancer tumor tissues was significantly lower than in early-stage tumors. This observation was further confirmed in high-grade gastric cancer cell line MKN45. The expression of CD44 …

Studying Redtail And Silver Surfperch In Sandy Beach Surf Zones With Mpas In Humboldt County, California, Usa, Noah G. Angell

Cal Poly Humboldt theses and projects

The sandy beach surf zone (SBSZ) represents an ecologically, economically, and culturally important marine habitat around the world. Despite its significance, the SBSZ has been historically underrepresented in marine and coastal ecology research. This in turn has caused many inshore marine species that prefer this habitat to be largely ignored. Two particular species, the Redtail surfperch Amphistichus rhodoterus and the Silver surfperch Hyperprosopon ellipticum, have not been well studied in the PNW region . There are merely three studies conducted on the Redtail surfperch in the Pacific Northwest region and the Silver surfperch has received even less research attention with …

Effect Of Plant Based Nutritional Education On A1c Levels In Type 2 Diabetic Adults, Candice Kim

Master of Science in Nursing Family Nurse Practitioner

Type 2 diabetic adults are those with a condition that prevents production of enough insulin and are also resistant to the effects of insulin itself (American Diabetes Association, 2018). According to the U.S. Department of Health and Human Services, “diabetes mellitus affects an estimated 29.1 million people in the United States, and is the 7th leading cause of death,” (Office of Disease Prevention and Health Promotion, 2022, section 3). Risk factors that affect type 2 diabetic adults include being overweight, aged 45 or older, high blood pressure, and high level of triglycerides (National Institute of Diabetes and Digestive and Kidney …

The Essence Of The Image Of The Fool In The Context Of The Orthodox Teachings In Eastern Europe, Olga Polumysna, Valeriy Bulatov

Occasional Papers on Religion in Eastern Europe

The article explores aspects of the image of the holy fool in the context of Orthodox teaching in Eastern Europe. The phenomenon of people who are called holy fools in the thinking and teaching of the Orthodox religious tradition is a controversial issue and still causes conflicting opinions, because the actions of people called holy fools are difficult to understand both for simple observers and for Orthodox researchers. The contrast of opinions in the formation of the holy fool's gestalt is of particular interest to this study. Is the life of holy fools directly connected with sacred life and Orthodox …

When Local Meets Global: Siberian Khanty Religious Traditions In The Everchanging World, Elena Glavatskaya

Occasional Papers on Religion in Eastern Europe

This article focuses on the ethnic history and religious traditions of the Khanty (formerly known as Ostiaks), a Finno-Ugric people dwelling in the north of Western Siberia and its responses to the global influences. With 40 other widely dispersed small peoples of the Russian North and Siberia, they are classified as aboriginal small-numbered people of the North and Siberia. The last three decades of their ethnic history was marked by a stormy process of ethnic and religious mobilization started in the context of the environmental crisis of the 1980-90s, which threatened their traditional land use. This article aims to analyze …

Mouralherwaqh Coastal Wetland Road Crossing Da'luk, Pakunihanich Nicoli Martin

Cal Poly Humboldt theses and projects

The integration of Indigenous and Western science plays an essential role in Tribally led collaborations for land management. This process of woven sciences is rooted in reciprocal relations and partnerships guided by Tribal Nations. Our cohort was invited by Wiyot Tribal Representatives to investigate a culvert located within the wetlands of Mouralherwaqh— a parcel of land reacquired by the Wiyot Tribe in 2022. This document seeks to share our experience and analysis as part of the Wiyot Tribe’s broader journey in navigating ecocultural restoration projects within Mouralherwaqh. The four community interests we investigated for the wetland crossing included a resized …

Comparing Alternative Developmental Modes: Structure And Gene Expression In The Olfactory System Of Plethodontid Salamanders, Giuseppina S. Lanzilli

Cal Poly Humboldt theses and projects

The olfactory system of extant amphibians changes as the animal transitions from a fully aquatic to a terrestrial lifestyle at metamorphosis. Cellular morphology of the nose and expression patterns of olfactory genes in the nasal cavity have been examined for a variety of frogs and salamanders, but among plethodontid salamanders, molecular data are available only for Plethodon shermani. Using standard histology and micro-CT reconstruction, I investigated the structure of the olfactory organs of larvae, juveniles, and adults of six plethodontid species, with terrestrial, streamside, semiaquatic, and aquatic adults. The overall structure of the olfactory cavity was generally similar across …

Evaluating The Utility Of Tracers To Characterize Environmental Dna Transport And Inform Detection Of Fishes In Small Streams, Gavin B. Bandy

Cal Poly Humboldt theses and projects

This study was motivated by the need to develop a noninvasive and highly sensitive monitoring tool for determining local occupancy of an endangered aquatic species To make inference into the occupancy of fishes within small stream sites, we developed a method to determine whether environmental DNA (eDNA) concentrations from a target species were elevated relative to a tracer, formulated to mimic eDNA and introduced at a single location. We examined patterns in the observed distribution of the tracer to account for the effects of site-specific transport processes and sampling on distributions of naturally occurring eDNA at small spatial scales (400 …

Beyond The Classroom: A Land-Based Approach To Outdoor Education, Jacquelyn R. Matthews

Cal Poly Humboldt theses and projects

This pilot project seeks to nurture space for Indigenous perspectives while equipping early childhood educators with tools for integrating diverse epistemology into outdoor learning practices to foster collaborative educational approaches. To align with this effort of shared knowledge, the project intends to offer a culturally sustaining educational tool that can help disrupt dominant approaches by evaluating alternative teaching strategies that center partnerships with local Indigenous communities to move toward equity-centered education. This can be achieved by modeling a resource guide after the theoretical underpinnings of Etuaptmumk Two-Eyed Seeing, and land-based pedagogy. A major component of this resource guide will include …

The Larval Preference Of An Invasive Bryozoan (Watersipora 'New Species') For Copper-Based Antifouling Paints, Alexander K. Strawhand

Cal Poly Humboldt theses and projects

The bryozoan Watersipora subtorquata and its cryptic congener Watersipora ‘new species’ are now globally invasive species. Previous studies have found this species complex to have a high tolerance to copper-based antifouling paints, which represent a large portion of the mitigation effort toward preventing hull fouling by invasive invertebrates. Tolerance alone, however, is unlikely to explain the full extent of Watersipora’s massive and rapid worldwide invasion success. The aim of this research was to move beyond tolerance, and test whether larvae of Watersipora ‘new species’ exhibit a preference for copper-coated settlement surfaces in both laboratory and field settings. For the laboratory …

A Case Of Incipient Budding Speciation In The California Floristic Province, Infraspecific Divergence In Abronia Villosa, Eli J. Allen

Cal Poly Humboldt theses and projects

Physical barriers to gene flow are the traditional evidence for species divergence. Conversely, there has been increasing acknowledgment of speciation in the face of gene flow as an evolutionary process. Budding speciation involves peripheral populations adapting to local ecological conditions, thereby budding off from a widespread progenitor species. Budding speciation is distinguished by ecological divergence and is generally evidenced by asymmetrical range size and nested phylogenetic relationships of sister species. The narrow endemic Abronia villosa var. aurita is adapted to montane sandy washes adjacent to its widespread sister variety, the desert dwelling var. villosa. Here, I tested the hypothesis …

Neurocinematics And Empathy: How Cognitive Neuroscience Enhances Our Understanding Of Emotional Responses Of Film, Kira Trinity

Cal Poly Humboldt theses and projects

Cinema is a medium that is beloved around the globe since its inception over a century ago. There have been speculations on how it is that cinema works, from editing to emotional processing of the story, but only recently have we begun to explore the inner workings of cinematic impact on the brain. In this paper we will review research on cinematic impact and define cinematic editing, discuss the birth of “neurocinematics”, highlight what we have observed with regards to neuroimaging and empathy when viewing films, and speculate on how our responses to cinema may be driven by the Mirror …

The Nature And Extent Of Algal Symbiosis In Three North American Ranids, Zachary T. Vegso

Cal Poly Humboldt theses and projects

The symbiotic relationship between the chlamydomonad green alga Oophila amblystomatis and embryos of certain amphibian species is often presumed to be mutualistic. However, the existence of a mutualism has only been experimentally tested and established in two closely related ambystomatid salamanders. These experiments showed a positive correlation between intracapsular algal density and embryonic growth, survival, hatching synchrony, and hatchling body size. Oophila has been documented within egg capsules of a growing number of amphibian species, including several frogs in the family Ranidae. However, the nature and extent of this relationship remains unclear. Ranid eggs are better oxygenated than ambystomatid eggs, …

Anxieties About The Future: Ecocriticism And Dystopian Landscapes In The Three Stigmata Of Palmer Eldritch And Selected Fiction By Philip K. Dick, Nickolas Michael Sykora

Cal Poly Humboldt theses and projects

In a literary analysis of selected fiction by Philip K Dick through an ecocritical framework, the focus of this study reveals the consequences of ecological destruction on futuristic societies reflecting the Anthropocene. Drawing correlations between various texts by the author, the separation of nature from humanity demonstrates how dystopian landscapes influence the identity of the characters in these settings and how dystopia serves as a prism which distorts or reflects what it means to be human. With this, ontology and artificial intelligence are analyzed as a notable facet of his literature which addresses the progress of innovation in society and …

Effects Of Habitat Selection And Individual Quality On The Reproductive Success Of Barn Owls (Tyto Furcata), Jaime E. Carlino

Cal Poly Humboldt theses and projects

The hypothesis of adaptive breeding habitat selection proposes congruence between the evolved selection of habitats and fitness components such as reproductive success. However, mismatches between habitat selection and observed fitness outcomes are not unusual and can arise for multiple reasons. Variation in individual qualities of animals has also been found to affect reproduction and survival. I examined associations between barn owl nest box selection, reproductive output, and plumage characteristics previously shown to correspond to individual quality. I found that barn owls selected tall nest boxes made of wood, mounted high off the ground, and with increased proportions of grassland around …

The Effects Of Native Perennial Cover On Physiological Indicators Of Habitat Quality For Sparrows In California Coastal Prairie Rangelands, Madeleine A. Ybarra

Cal Poly Humboldt theses and projects

Prescribed grazing management can have myriad effects on wildlife, with low to moderate grazing being associated with an increased abundance of some grassland birds. Specifically, Grasshopper Sparrow (Ammodramus savannarum) and Savannah Sparrow (Passerculus sandwichensis) abundance have been shown to be positively associated with both grazing and a higher percentage of native plant cover. However, measures of abundance provide an incomplete assessment of habitat quality for birds. Physiological and morphological measurements can help indicate environmental stress in individual birds, which reveals more information about habitat quality. We measured body condition and heterophil:lymphocyte ratios to gauge avian stress …

Impact Of Oxytocin And Tetrahydrocannabinol On Signs Of Fear In The Rat, Jair E. Alvarez Jr.

EWU Masters Thesis Collection

BACKGROUND: Oxytocin (OXT) has been used as a post-treatment for post-traumatic stress disorder (PTSD). Previous research suggests that pre-treatment of intranasal OXT attenuates signs of fear in rats; however, the impact of intraperitoneal (i.p) OXT pre-treatment on signs of fear in rats is unknown. We also explored the effects of delta-9 (psychoactive component of cannabidiol) and delta-8 (delta-9 isomer) THC pre-treatment on attenuating signs of fear in the rat. METHODS: For each experiment, male Sprague-Dawley rats were divided into 5 groups, (n=8 per group); 1.) controls (vehicle and no foot shock), 2.) shock (vehicle and foot shock), 3.) low dose …

Using Novel And Traditional Survey Techniques To Monitor Small Mammal Species In Northwestern California, Sydney Mccluskey

Cal Poly Humboldt theses and projects

Small mammal species play a critical role in forest ecosystems contributing significantly to overall forest biodiversity and ecological dynamics. Forest-dwelling species are among the most vulnerable to habitat loss and fragmentation. It is essential to develop efficient techniques for monitoring small mammal populations in forested ecosystems to inform conservation efforts and management plans; yet, monitoring small mammals in these habitats can be particularly challenging due to their size, activity patterns, and frequently nocturnal nature. In this study, I aimed to address two primary research objectives: 1) compare the effectiveness of 3 distinct camera-trap survey techniques (i.e., ground, tree, and tube) …

The "Upside-Down" River: Trends In Total Nitrogen, Total Phosphorus, And Microcystin Concentration In The Klamath Basin, 2010-2021, Kayla M. Fitzpatrick

Cal Poly Humboldt theses and projects

The Klamath River tends to experience impaired upstream water quality, which improves downstream. Although this consensus is accepted, an updated spatiotemporal analysis of certain water quality parameters has not been conducted since 2017. With four of the Klamath River’s dams slated for removal by the end of 2024, it is critical to have an updated assessment of current water quality conditions from which to compare conditions following dam removal. In this study, total nitrogen (TN), total phosphorus (TP), and microcystin data from 2010-2021 were analyzed to determine concentration trends from Link Dam to the Klamath River Estuary.

Concentrations of TN …

Exploring Colorism In Children’S Literature: Sulwe And Black Is Brown Is Tan, Jasmine April Fortunato

Cal Poly Humboldt theses and projects

My analysis of the texts Sulwe by Lupita Nyong'o and Black Is Brown Is Tan by Arnold Adoff works to evaluate the representation of colorism in children’s literature to understand how it is represented and has evolved in its messaging towards a young audience. Using the frameworks of Critical Race Theory, Critical Skin Theory and Color Blindness under a lens of colorism, my research utilizes visual analysis to view how it is physically conveyed and research in the literacy of children in understanding intersectional issues. Utilizing literary analysis, I analyze the ways colorism is discussed and combatted through comparisons, representation, …

Scale And Habitat Effects On Measurement Of Streptomyces Biogeography And Biodiversity, Terilyn A. Stoflet

Cal Poly Humboldt theses and projects

Bacteria in the genus Streptomyces are detected in most soils around the world, as well as on and in seeds, plants, animals, insects and the wind. Currently, more than 700 valid species of Streptomyces have been identified worldwide. Streptomyces produce the majority of antibiotics, antivirals and antifungals, as well as important agricultural bioactive molecules due to their unique genetic capabilities. Their distinct genetic abilities allow them to produce an unlimited amount of biosynthetic gene clusters, or BGC’s. Although many compounds come from Streptomyces, scientists have not discovered the biogeographic patterns of this genus. The biogeography of Streptomyces is a …