Digital Commons Network^™

Engineering E. Coli Strains Using Antibiotic-Resistance-Gene-Free Plasmids, Matthew B Amrofell, Sunaina Rengarajan, Steven T Vo, Erick S Ramirez Tovar, Larissa Lobello, Gautam Dantas, Tae Seok Moon

2020-Current year OA Pubs

We created a generalizable pipeline for antibiotic-resistance-gene-free plasmid (ARGFP)-based cloning using a dual auxotrophic- and essential-gene-based selection strategy. We use auxotrophic selection to construct plasmids in engineered E. coli DH10B cloning strains and both auxotrophic- and essential-gene-based selection to (1) select for recombinant strains and (2) maintain a plasmid in E. coli Nissle 1917, a common chassis for engineered probiotic applications, and E. coli MG1655, the laboratory "wild-type" E. coli strain. We show that our approach has comparable efficiency to that of antibiotic-resistance-gene-based cloning. We also show that the double-knockout Nissle and MG1655 strains are simple to transform with plasmids …

Nyls At The Association Of American Law Schools Annual Meeting, Dean Anthony W. Crowell

NYLS Community News

No abstract provided.

Streptococcus Intermedius: From A Normal Oral Commensal To A Life-Threatening Organism, Anas Mahmoud, Tala Beilani, Nizar Alyassin, Karam Zakharia, Taha Basil, David Poulad

Student Publications

Subdural empyema is a collection of pus in the subdural space between the dura mater and the arachnoid. It carries very high morbidity and mortality as it can spread anywhere in the brain; however, the risk can be mitigated with appropriate surgical and medical intervention. Being protected by the skull, cranial infections are usually preceded by a significant risk factor, either an external invader such as skull fractures secondary to trauma, penetrating injury, prior surgery, or, more commonly, in more than 50% of cases, due to spread of an internal infection such as ear or sinus infections. Anaerobic and aerobic …

How Should Complicated Cases Of Thrombotic Thrombocytopenic Purpura With Positive Coombs Test Be Treated?, Moutaz Ghrewati, Anas Mahmoud, Tala Beilani, Karam Zakharia, Mehandar Kumar

Student Publications

Thrombocytopenia with concomitant anemia is a serious condition with a high mortality risk. Destruction of platelets, i.e., thrombocytopenia, can be secondary to either auto-antibodies (immune-mediated) or mechanical destruction (non-immune-mediated). The Coombs test is a widespread tool to differentiate between the two categories, resulting in different specific treatment approaches for each diagnosis. A peripheral blood smear can also help make the diagnosis; for instance, in cases of mechanical destruction such as thrombotic thrombocytopenic purpura (TTP), the red blood cell (RBC) shape looks fragmented, forming schistocytes. In rare instances, TTP can present with both schistocytes and a positive Coombs test, challenging the …

Experiences Of Caregivers Of People With Dementia In Indonesia: A Focus Group Study, Imelda Theresia, Tara P. Sani, S Evans‐Lacko, Nicolas Farina, Lydia Augustina, Yuda Turana

Peninsula Medical School

Abstract Background Many people with dementia are reliant on family caregivers to provide daily care to maintain quality of life and dignity. As a result, caregivers can experience increased burden, poorer health outcomes and increased stigma. To date, the experiences of caregivers of people with dementia has not been explored within an Indonesian context. Aims This study aims to understand the experience of caregivers of people with dementia in Indonesia and better understanding of the stigma associated with dementia. Materials and Methods This qualitative study is embedded within the Strengthening Responses to Dementia in Developing Countries project. Focus Group Discussions …

A Jacket-Frame Mounted Oscillating Water Column With A Variable Aperture Skirt, Carlos Pérez-Collazo, Deborah M. Greaves, Gregorio Iglesias

School of Engineering, Computing and Mathematics

During the last decade jacket-frames have emerged as the main kind of substructure for bottom-mounted offshore wind farms in intermediate water depths. With the offshore wind industry moving towards deeper waters, the predominance of jacket-frames is expected to increase in future years. Multipurpose platforms combining wind and wave energy are proposed as an innovative solution to enhance the sustainability of offshore wind energy. In this research, a multipurpose platform is investigated with a novel feature in its oscillating water column (OWC) wave energy converter—a variable geometry skirt. A comprehensive physical modelling campaign was carried out using a 1:50 scale model. …

Limit Distributions Of Products Of Independent And Identically Distributed Random 2 × 2 Stochastic Matrices: A Treatment With The Reciprocal Of The Golden Ratio, Santanu Chakraborty

School of Mathematical & Statistical Sciences Faculty Publications

Consider a sequence (Xn)n≥1 of i.i.d. 2×2 stochastic matrices with each Xn distributed as μ. This μ is described as follows. Let (Cn,Dn)T denote the first column of Xn and for a given real r with 012 is very challenging. Considering the extreme nontriviality of this case, we stick to a very special such r, namely, r=√5−12 (the reciprocal of the golden ratio), briefly mention the challenges in this nontrivial case, and completely identify λ for a very special situation.

On The Use Of Response Conditioned Focused Wave And Wind Events For The Prediction Of Design Loads, Tom Tosdevin, Emma Edwards, Anna Holcombe, Scott Brown, Edward Ransley, Martyn Hann, Deborah Greaves

School of Engineering, Computing and Mathematics

Reducing the time required to predict design loads and responses would result in significant efficiency improvements to the floating offshore wind turbine (FOWT) design process. Present methods of predicting design values require at least six, 1-hour sea condition simulations. It would therefore be highly beneficial to identify what conditions lead to the extremes of a range of responses of interest to reduce the length of the time series required. The results of physical, 1:70 scale model experiments using the UMaine VolturnUS-S platform and IEA 15MW reference wind turbine are presented. A Real-time hybrid testing approach, where wind loading is calculated …

Challenges And Opportunities In Insulin Resistance-Induced Cognitive Dysfunction, Nancy Abdel-Kareem, Shimaa Elshazly, May Galal, Enas Ahmed Abd El-Haleim

Bulletin of Faculty of Pharmacy Cairo University

In the last few years, there has been a lot of interest in the effect of nutrition on cognitive function, as it affects insulin signals inside the body, whose role is not limited to controlling blood glucose levels but also extends to the learning process and cognitive function. Nutrition-induced insulin resistance is one of the present diseases, which impairs brain insulin signaling and leads to various forms of dementia. This review discusses the different roles of insulin inside the body, the negative effect of insulin resistance on brain function, and the use of some drugs that may improve cognitive dysfunction.

Review Of Fighting Better, Kriesberg, Oxford 2023, Neil Katz

Peace and Conflict Studies

No abstract provided.

Conflict And Choice Of Study At University Level: Evidence From Pakistan, Abbas Ali Gillani, Xiaocheng Hu

Peace and Conflict Studies

Conflict, and violence related events have been found to have significant effects on the cognitive thinking and mental well-being of individuals. Although there is ample evidence suggesting negative association of conflict with schooling outcomes, there is non-existent research on how violence can impact degree choices made by students at the university level. By using university level admissions data between 2014 and 2016 from Pakistan, this paper examines the differential in preference for degree choices of students who live in conflict-affected areas compared to students who live in conflict-free areas. The results show that students exposed to violence were less likely …

War Or Peace Discourse? Analysis Of News Headlines On Pulwama Attack, Showkat Ahmad Jan Mr, Francis Philip Barclay Dr

Peace and Conflict Studies

Discourse analysis and the theories of war and peace journalism are used to investigate the newspaper coverage of the Pulwama attack of 2019, a recent development in the Kashmir conflict between India and Pakistan that brought the nuclear-armed Asian neighbours on the brink of a war. To identify the journalistic discourses of the Pulwama attack, 686 headlines of news articles published in six popular newspapers on the attack and its aftermath during February 15-25, 2019, are analysed. Three levels of discourse analysis are employed: Lexical, social and ideological stratifications. Under lexical stratification, the news headlines are analysed to identify their …

The Peace Is Uneasy And Needs To Be Won Everyday: Economic Assistance And Peacebuilding Among Marginalized Groups In Northern Ireland And The Border Area, Mehmet Yavuz, Sean Byrne

Peace and Conflict Studies

While Northern Ireland is still recovering from the violent legacy of the 30-year Troubles during a global COVID-19 pandemic, people still experience violence daily. Economic and political inequalities heighten intergroup tensions and insecurity contributing to the promotion of destructive stories. Brexit has escalated the culture wars between the Protestant Unionist Loyalist and Catholic Nationalist Republican communities as Loyalist youth and paramilitary groups protest on Belfast’s streets. Through 120 semi-structured interviews, we explore the experiences and understandings of Civil Society Organization (CSO) peacebuilding and reconciliation workers that liaison with ex-combatants and youth in Derry and the Border area of Northern Ireland. …

Food And Commensality In Non-State Armed Groups: The Case Of The Lord's Resistance Army In Northern Uganda, 1987-2008, Eunice Otuko Apio Dr

Peace and Conflict Studies

The subfield of food and eating practices has registered a significant volume of theoretical and empirical studies. However, there is very limited research targeting non-state armed groups. This article contributes to understanding the nuanced role of food and eating practices (or commensality) in conflict, and its significance in the construction and sustenance of sense of community in non-state armed groups that use particularly elaborate means of indoctrination to build a following. Drawing on the case of northern Uganda’s Lord’s Resistance Army (LRA) that thrived on mass abduction of children, youth and women between 1987 and 2008, this article argues that …

The Role Of Local Agency In Peacebuilding: The Case Of The Bawku Inter-Ethnic Peace Committee, Gamel M. A. Aganah Phd

Peace and Conflict Studies

This article explores the failure of top-level interventions in the resolution of local conflicts and the role of local agency in peacebuilding. The article relied primarily on qualitative techniques and instruments to collect data to examine why top-level interventions by successive governments have failed to resolve the Bawku chieftaincy conflict. The article finds that top-level interventions have failed to resolve the conflict because they are introduced with little to no participation by local communities. These interventions are, therefore, unable to garner grassroot support and commitment. In contrast, the BIEPC led peace process has been more successful in the management of …

Front Matter

Kentucky English Bulletin

No abstract provided.

Policy And Text Selection In The Secondary Ela Classroom, Jessica Winters

Kentucky English Bulletin

No abstract provided.

The Tree Of Taking, Austin Snyder

Kentucky English Bulletin

No abstract provided.

Crt In Schools, Latoya Lee

Kentucky English Bulletin

No abstract provided.

Student Perception Of Crime And The Role Of School Resource Officers, Olivia Dunn

Honors Program Theses and Projects

The increased use of school safety measures and Zero tolerance policies in the United States education system has created a disconnect between perceived and actual school safety. While legislators, school administrators, and the public fuel the creation of school safety guidelines, the voice of the student population these guidelines impact may have been lost. The purpose of this study was to investigate student perceptions of crime, and the role of School Resource Officers (SROs) based on their high school experience. This project was conducted to help understand the factors that contribute to student perceptions and how school security measures may …

Influence Of Gender Identity On The Adoption Of Religious-Spiritual, Preventive And Emotion-Focused Coping Strategies During The Covid-19 Pandemic In Pakistan, Saeed Ahmad, Sara Rizvi Jafree

Sociology and Anthropology Student Research

Background

Academic research has highlighted the gendered impacts and amplifications of gender disparities of COVID-19. Traditionally, Pakistan is a patriarchal society, where it is a parenthood norm to socialize specific gender social roles.

Objectives

The current research asserts that these normative gender roles may influence individuals throughout their life course, even during the COVID-19 pandemic. Therefore, the present study explored the influence of gender identity in adopting different coping strategies such as religious-spiritual, preventive, emotion-focused and non-constructive coping against the COVID-19 pandemic.

Methods

Due to the lockdown in various areas of Pakistan, data were collected through an online questionnaire using …

Cross-Seasonal Effects In A Sea Ice-Associated Sea Duck: Do Winter Conditions Affect Breeding Spectacled Eiders?, Randall J. Friendly

Biological Sciences

Climate change in the Arctic is more rapid than anywhere on the globe and changes in the marine environment can impact the distribution and abundance of Arctic and sub-Arctic species. Understanding how a species responds to climate change can aid conservation planning and recovery. Spectacled eiders (Somateria fischeri), sea ducks listed as "threatened" under the Endangered Species Act, winter at the Bering Sea and nest along the coastal areas of Alaska and Arctic Russia. Severity of winter conditions in the Bering Sea have been associated with both reduced annual survival and reduced breeding abundance and may have sublethal effects during …

A Closer Look At Our Ever-Changing Solar System, Erik Johnson

Natural Science Faculty

What is a planet? It's a question that was controversial when Pluto was reclassified in 2006. The definition has changed over time as people have learned more about the universe. There are still unresolved differences between the cultural usage and its various scientific uses.

Nursing Care Of The Pediatric Patient With Autism, Cayley Frolik

Honors Capstones

The purpose of this project is to help educate current and future nurses on ways to understand and better connect with pediatric patients with autism. Standard nursing curriculum tends to focus primarily on the clinical approach for mainstream patients. In one short year, I recognized a glaring need for nurses to learn how to care for those with autism, as well as how to support and educate their families to create the best environment for whole success. It is very difficult to understand the needs of these specific patients without the proper toolkit. It is my intention to research and …

The 2023 Zezima Christmas Letter, Jerry Zezima

Erma Bombeck Writers' Workshop Blog

Since I am in the holiday spirit (and, having just consumed a mug of hot toddy, a glass of eggnog and a nip of cheer, the holiday spirits are in me), I have decided to follow in that great tradition of boring everyone silly by writing a Christmas letter.

Ddx173.2 User Centred Design: Ui Design Example 2, Sae University College

Exemplars

The presentation showcases how the website's user interface (UI) design responds to the findings of the user experience (UX) report and improves its usability.

Pollution Trapping By Strong Temperature Inversions In Fairbanks, Alaska, Meeta V. Cesler-Maloney

Chemistry and Biochemistry

In cold climates during winter, surface-based temperature inversions reduce vertical dispersion within the atmospheric boundary layer. Reduced vertical dispersion coupled with stagnant horizontal winds causes pollution emitted near the ground level to accumulate to unhealthy amounts. To study pollution trapping, we measured vertical differences in fine particulate matter and ozone across a shallow 20-meter vertical scale during surface-based inversions in Fairbanks, Alaska and showed that pollution trapping occurs on this extremely shallow scale. In winter 2022, trace gases were measured by long-path differential absorption spectroscopy to probe vertical dispersion on a taller scale, up to ~200 m above the surface. …

From Siberian Dawn To Midnight In Siberia: The Geopolitics Of Anglophone Travel Writing About Post-Soviet Siberia, Benjamin D. Christian

Arctic and Northern Studies

The collapse of the Soviet Union in 1991 opened Siberia as a new "travelers' frontier." Travel writers seeking destinations unspoiled by the encroachment of global capitalism and the resultant "placeless" landscapes made Siberia the subject of numerous travel books. This thesis critically examines the geopolitical discourses embedded in those books. Through a close reading and discourse analysis of three representative samples spanning the post-Cold War period, it highlights the key tropes, images and discourses that Anglophone travel writers have deployed in their constructions of Russia as a geopolitical Other. Travel writers writing about Siberia during the post-Cold War period explicitly …

Indigenous Self-Determination In Co-Production Of Knowledge, Margaret Hope Rudolf

Anthropology

Analyzing and comprehending co-production of knowledge (CPK) in the context of working with Arctic Indigenous communities on climate change research is the main goal for this interdisciplinary doctoral dissertation. CPK is shared decision-making on every step of the research process with research partners from communities, agencies, or organizations. CPK with Arctic Indigenous communities requires dedicated consideration of equity, ethics, cultural worldviews, and colonization. Key concepts from Indigenous critical methodologies are used to analyze both the CPK theory and implementation. CPK has the potential to be an ethical space to question the status quo of research processes and support Indigenous self-determination. …

Preserving Reflections Of Ourselves: The Past, Present, And Future Of Alaska's Museums, Angela J. Linn

Anthropology

While museums are very good at collecting, preserving, documenting, and interpreting the histories of our communities, we have not done a very good job with our own histories. No comprehensive publication holistically examines the development of museums in Alaska, let alone looks critically at the "big three" (Alaska State Museums, University of Alaska Museum of the North, and the Anchorage Museum) with a goal of establishing a connection between the historical context, the individuals who shaped those museums, and the institutions' current states of being. This dissertation uses the academic fields of history, museology, and ethnography to discover and analyze …