Digital Commons Network^™

Selective Reprogramming Of Regulatory T Cells In Solid Tumors Can Strongly Enhance Or Inhibit Tumor Growth, Rami Alfar, John V Napoleon, Imrul Shahriar, Richard Finnell, Cole Walchle, Austin Johnson, Philip S Low

Faculty, Staff and Students Publications

Folate receptor delta (FRδ) has been used as a biomarker for regulatory T cells (Tregs), because its expression is limited to Tregs and ovum. Although FRδ is unable to bind folate, we have used molecular docking software to identify a folate congener that binds FRδ with high affinity and have exploited this FRδ-specific ligand to target attached drugs (imaging agents, immune activators, and immune suppressors) specifically to Tregs in murine tumor xenografts. Analysis of treated tumors demonstrates that targeting of a Toll-like receptor 7 agonist inhibits Treg expression of FOXP3, PD-1, CTLA4, and HELIOS, resulting in 40-80% reduction in tumor …

The Nr2f2-Hand2 Signaling Axis Regulates Progesterone Actions In The Uterus At Early Pregnancy, Yeongseok Oh, Elvis Quiroz, Tianyuan Wang, Yassmin Medina-Laver, Skylar Montague Redecke, Francisco Dominguez, John P Lydon, Francesco J Demayo, San-Pin Wu

Faculty, Staff and Students Publications

Endometrial function is dependent on a tight crosstalk between the epithelial and stromal cells of the endometrium. This communication is critical to ensure a fertile uterus and relies on progesterone and estrogen signaling to prepare a receptive uterus for embryo implantation in early pregnancy. One of the key mediators of this crosstalk is the orphan nuclear receptor NR2F2, which regulates uterine epithelial receptivity and stromal cell differentiation. In order to determine the molecular mechanism regulated by NR2F2, RNAseq analysis was conducted on the uterus of

Binary Classification Of The Endocrine Disrupting Chemicals By Artificial Neural Networks, Zahir Aghayev, George F Walker, Funda Iseri, Moustafa Ali, Adam T Szafran, Fabio Stossi, Michael A Mancini, Efstratios N Pistikopoulos, Burcu Beykal

Faculty, Staff and Students Publications

We develop a machine learning framework that integrates high content/high throughput image analysis and artificial neural networks (ANNs) to model the separation between chemical compounds based on their estrogenic receptor activity. Natural and man-made chemicals have the potential to disrupt the endocrine system by interfering with hormone actions in people and wildlife. Although numerous studies have revealed new knowledge on the mechanism through which these compounds interfere with various hormone receptors, it is still a very challenging task to comprehensively evaluate the endocrine disrupting potential of all existing chemicals and their mixtures by pure

Size Distributions Of Intracellular Condensates Reflect Competition Between Coalescence And Nucleation, Daniel S W Lee, Chang-Hyun Choi, David W Sanders, Lien Beckers, Joshua A Riback, Clifford P Brangwynne, Ned S Wingreen

Faculty, Staff and Students Publications

Phase separation of biomolecules into condensates has emerged as a mechanism for intracellular organization and affects many intracellular processes, including reaction pathways through the clustering of enzymes and pathway intermediates. Precise and rapid spatiotemporal control of reactions by condensates requires tuning of their sizes. However, the physical processes that govern the distribution of condensate sizes remain unclear. Here we show that both native and synthetic condensates display an exponential size distribution, which is captured by Monte Carlo simulations of fast nucleation followed by coalescence. In contrast, pathological aggregates exhibit a power-law size distribution. These distinct behaviours reflect the relative importance …

Assessment Of The Dimensionless Groups-Based Scale-Up Of Gas–Solid Fluidized Beds, Faraj M. Zaid, Haider Al-Rubaye, Thaar M. Aljuwaya, Muthanna H. Al-Dahhan

Chemical and Biochemical Engineering Faculty Research & Creative Works

The most common scale-up approach for gas–solids fluidized beds is based on matching the governing dimensionless parameters. in the literature, this approach has been validated only by means of measuring global parameters between different sizes of fluidized beds. However, such global measurements are not sufficient to depict all the interplaying hydrodynamic phenomena and hence verify the scale-up relationships. Therefore, to assess this approach, an advanced gas–solids optical probe and pressure transducer measurement techniques have been applied to quantify local hydrodynamic parameters in two different sized fluidized beds. Four different sets of experimental conditions were designed and conducted to examine the …

Reactive Oxygen Species (Ros)-Responsive Size-Reducible Nanoassemblies For Deeper Atherosclerotic Plaque Penetration And Enhanced Macrophage-Targeted Drug Delivery, Jianhua He, Wenli Zhang, Xiaoju Zhou, Fengfei Xu, Jiahui Zou, Qiqi Zhang, Yi Zhao, Hongliang He, Hu Yang, Jianping Liu

Chemical and Biochemical Engineering Faculty Research & Creative Works

Nanoparticle-Based therapeutics represent potential strategies for treating atherosclerosis; however, the complex plaque microenvironment poses a barrier for nanoparticles to target the dysfunctional cells. Here, we report reactive oxygen species (ROS)-responsive and size-reducible nano assemblies, formed by multivalent host-guest interactions between β-cyclodextrins (β-CD)-anchored discoidal recombinant high-density lipoprotein (NP3ST) and hyaluronic acid-ferrocene (HA-Fc) conjugates. the HA-Fc/NP3ST nano assemblies have extended blood circulation time, specifically accumulate in atherosclerotic plaque mediated by the HA receptors CD44 highly expressed in injured endothelium, rapidly disassemble in response to excess ROS in the intimal and release smaller NP3ST, allowing for further plaque penetration, macrophage-targeted cholesterol efflux and …

Emerging Challenges To Cellular Therapy Of Cancer, Premal D Lulla, Malcolm Brenner

Faculty, Staff and Students Publications

Cellular immunotherapy of cancer in the form of chimeric antigen receptor-modified T-cell therapy has become a standard treatment for lymphoid and more recently plasma cell malignancies. Although their successes in these cancers represent a breakthrough for adoptive cell therapy, there are several challenges to their continued growth in the field of cancer medicine. In this review, we discuss the progress made thus far toward achieving "off-the-shelf" accessibility of cell therapies that has the potential to greatly offset the costs associated with the current practice of making patient-specific products. We also review the innovations under investigation that attempt to make cellular …

Russia's Roulette: Sanctions, Strange Contracts & Sovereign Default, Lev E. Breydo

Faculty Publications

This Article is the first comprehensive, multi-disciplinary analysis of Russia’s sovereign debt and the consequences of a potential default.

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This Article introduces a Russian debt taxonomy divided into four distinctive categories. Starting with relatively standard terms in late-1990s vintage bonds, over time and as a close function of geo-political developments, the contracts grew unusual—bordering towards lawless.

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The rest of this Article is organized in four parts. Part II provides critical background regarding Russia’s sovereign debt and details key legal provisions likely implicated in the event of a default. Part III discusses how, due to the complex interplay between …

Political Default. The Implications Of Weaponizing Global Financial Infrastructure, Lev E. Breydo

Faculty Publications

In response to Russia’s unlawful invasion of Ukraine, the U.S. has led a broad-based global coalition to punish the aggressor with an “unprecedented” sanction regime. Those measures have targeted “the Russian government’s basic tools to manage its macroeconomy,” with a particular emphasis on its sovereign debt. That concerted focus, as this Article empirically demonstrates through analysis of bond and credit default swap data, ultimately resulted in Russia’s first foreign currency debt default in over a century — despite the nation’s ability and seeming willingness to pay.

Notwithstanding aptly-deserved “just deserts” considerations, a forced — or “political” — Russian sovereign debt …

Sustainability Exchange Dutchtown South Community Corporation 2023 Final Report, Miao Hu, Sarah Kim, Jordan Lin, Wilson Tryon

Sustainability Exchange Projects

No abstract provided.

Attitude Of Lis Postgraduate Students Towards The Use Of Remote Teaching And Learning At Post Covid-19 Era In Nigerian Private Universities, Saheed Abiola Hamzat Dr.

Library Philosophy and Practice (e-journal)

No abstract provided.

Misogynistic Extremism: A Scoping Review, Robin O'Hanlon, Frederick L. Altice, Roy Ka-Wei Lee, Jack Laviolette, Gloria Mark, Orestis Papakyriakopoulos, Koustuv Saha, Munmun De Choudhury, Navin Kumar

Publications and Research

In recent years, the concept of “misogynistic extremism” has emerged as a subject of interest among scholars, governments, law enforcement personnel, and the media. Yet a consistent understanding of how misogynistic extremism is defined and conceptualized has not yet emerged. Varying epistemological orientations may contribute to the current conceptual muddle of this topic, reflecting long-standing and on-going challenges with the conceptualization of its individual components. To address the potential impact of misogynistic extremism (i.e., violent attacks), a more precise understanding of what this phenomenon entails is needed. To summarize the existing knowledge base on the nature of misogynistic extremism, this …

Futures On Dry Ground: Anthropology And Coastal Planning, Theodore Hilton, Sheehan Moore

Publications and Research

Around the world, governments, industry, and other actors are creating plans to save coasts from environmental crisis. Louisiana is one prominent example: levees and other measures protect oil and gas infrastructure from inundation as the wetlands buffer rapidly erodes—in large part due to that same industry. The state’s primary answer to land loss is a $50 billion Coastal Master Plan. To illuminate such responses in Louisiana and globally, this article reviews emerging literature and frames an anthropology of coastal planning around three themes: (1) novel orientations toward time and space, (2) the reproduction of power and capital in the name …

Dental Students’ Perception Of Their Educational Environment In Relation To Their Satisfaction With Dentistry Major: A Cross‑Sectional Study, Yoon Min Gil, Ji Soo Hong, Ju Ly Ban, Jae‑Sung Kwon, Jae‑Il Lee

Publications and Research

Background: Students’ perception of their educational environment and satisfaction with their major can reveal the extent of their readiness to practice their profession after graduation. This study aimed to evaluate dental students’ perception of their educational environment and satisfaction with their major in dentistry, as well as the relationship between these two factors.

Methods: An online survey was conducted in 2022 among first- to fourth-year students across 11 dental schools in Korea. The Dundee Ready Education Environment Measure (DREEM) and Academic Major Satisfaction Scale (AMSS) were used to measure the students’ perception of the educational environment and satisfaction with their …

Changes To Sea Surface Temperatures And Vertical Wind Shear And Their Influence On Tropical Cyclone Activity In The Caribbean And The Main Developing Region, Keneshia Hibbert, Equisha Glenn, Thomas M. Smith, Jorge E. González-Cruz

Publications and Research

Sea surface temperatures and vertical wind shear are essential to tropical cyclone formation. TCs need warm SSTs and low shear for genesis. Increasing SSTs and decreasing VWS influences storm development. This work analyzes SST and VWS trends for the Caribbean, surrounding region, and the Atlantic hurricane main developing region from 1982–2020. Storm intensity increases significantly during this period. Annual and seasonal trends show that regional SSTs in the MDR are warming annually at 0.0219 °C yr⁻¹ and, per season, 0.0280 °C yr⁻¹. Simultaneously, VWS decreases during the late rainfall season, at 0.056 m/s yr⁻¹ in the …

Distinctions Between Choroidal Neovascularization And Age Macular Degeneration In Ocular Disease Predictions Via Multi-Size Kernels Ξcho-Weighted Median Patterns, Alex Liew, Sos Agaian, Samir Benbelkacem

Publications and Research

Age-related macular degeneration is a visual disorder caused by abnormalities in a part of the eye’s retina and is a leading source of blindness. The correct detection, precise location, classification, and diagnosis of choroidal neovascularization (CNV) may be challenging if the lesion is small or if Optical Coherence Tomography (OCT) images are degraded by projection and motion. This paper aims to develop an automated quantification and classification system for CNV in neovascular age-related macular degeneration using OCT angiography images. OCT angiography is a non-invasive imaging tool that visualizes retinal and choroidal physiological and pathological vascularization. The presented system is based …

Quantity Of Questing Black-Legged Ticks And Associated Micro-Scale Environmental Data Collected From Four Suburban Parks Near New York City, Shipeng Sun, Chong Di, Li Li, Brian Sulkow, Weigang Qiu

Publications and Research

During 2017 and 2018, we collected the quantity of questing black-legged ticks (Ixodes scapularis), also known as deer ticks, in 124 sampling sites of 5m by 5m in four state parks—Caumsett State Historic Park, Connetquot River State Park, Rockefeller State Park, and Fire Island National Seashore—around New York City. The black-legged tick is the primary vector for the spirochete Borrelia burgdorferi, the pathogen of Lyme disease, in Northeastern United States. Using the flagging method, we collected and counted the numbers of adult and nymphal black-legged ticks at each site. Along with these quantities, we also recorded the …

Automated Morphological Phenotyping Using Learned Shape Descriptors And Functional Maps: A Novel Approach To Geometric Morphometrics, Oshane O. Thomas, Hongyu Shen, Ryan L. Raaum, William E. H. Harcourt-Smith, John D. Polk, Mark Hasegawa-Johnson

Publications and Research

The methods of geometric morphometrics are commonly used to quantify morphology in a broad range of biological sciences. The application of these methods to large datasets is constrained by manual landmark placement limiting the number of landmarks and introducing observer bias. To move the field forward, we need to automate morphological phenotyping in ways that capture comprehensive representations of morphological variation with minimal observer bias. Here, we present Morphological Variation Quantifier (morphVQ), a shape analysis pipeline for quantifying, analyzing, and exploring shape variation in the functional domain. morphVQ uses descriptor learning to estimate the functional correspondence between whole triangular meshes …

Adaptive Single Low-Light Image Enhancement By Fractional Stretching In Logarithmic Domain, Thaweesak Trongtirakul, Sos S. Agaian, Shiqian Wu

Publications and Research

Low-light image enhancement is a challenging task that aims to improve the visibility and quality of images captured in dark environments. However, existing methods often introduce undesirable artifacts such as color distortion, halo effects, blocking artifacts, and noise amplification. In this paper, we propose a novel method that overcomes these limitations by using the logarithmic domain fractional stretching approach to estimate the reflectance component of the image based on the improved Retinex theory. Moreover, we apply a simple adaptive gamma correction algorithm to the Lab color-space to adjust the brightness and saturation of the image. Our method effectively reduces the …

Lead Induced Differences In Bone Properties In Osteocalcin +/+ And −/− Female Mice, G. Yildirim, W. C. Budell, O. Berezovska, S. Yagerman, S. S. Maliath, P. Mastrokostas, S. Tommasini, T. L. Dowd

Publications and Research

Lead (Pb) toxicity is a major health problem and bone is the major reservoir. Lead is detrimental to bone, affects bone remodelingand is associated with elderly fractures. Osteocalcin (OC) affects bone remodeling, improves fracture resistance and decreases with age and in some diseases. The effect of lead in osteocalcin depleted bone is unknown and of interest. We compared bone mineral properties of control and Pb exposed (from 2 to 6 months) femora from female adult C57BL6 OC+/+ and OC−/− mice using Fourier Transform Infrared Imaging (FTIRI), Micro-computed tomography (uCT), bone biomechanical measurements and serum turnover markers (P1NP, CTX). Lead significantly …

Feeling Analogue: Using Modular Synthesizers, Designing Synthesis Communities, Eliot Bates

Publications and Research

When Harald Bode nominally invented the modular synthesiser in 1960, it was not yet clear whether it was a musical instrument or a less-defined form of studio-sited audio technology (Subotnik 1970). Much of the technology, from the interface design to the specific parts, was shared with nonmusical test and measurement equipment. Sixty years later, hardware modular synthesis is used not just for musical creation but for noise, sound art and data sonification/visualization. Through an ethnographic study of the design and use of the Eurorack modular synthesis format, and of modular synthesis maker and user communities spread through North America, Europe …

I2n: Image To Nutrients, A Sensor Guided Semi-Automated Tool For Annotation Of Images For Nutrition Analysis Of Eating Episodes, Tonmoy Ghosh, Megan A Mccrory, Tyson Marden, Janine Higgins, Alex Kojo Anderson, Christabel Ampong Domfe, Wenyan Jia, Benny Lo, Gary Frost, Matilda Steiner-Asiedu, Tom Baranowski, Mingui Sun, Edward Sazonov

Children’s Nutrition Research Center Staff Publications

Introduction: Dietary assessment is important for understanding nutritional status. Traditional methods of monitoring food intake through self-report such as diet diaries, 24-hour dietary recall, and food frequency questionnaires may be subject to errors and can be time-consuming for the user.

Methods: This paper presents a semi-automatic dietary assessment tool we developed - a desktop application called Image to Nutrients (I2N) - to process sensor-detected eating events and images captured during these eating events by a wearable sensor. I2N has the capacity to offer multiple food and nutrient databases (e.g., USDA-SR, FNDDS, USDA Global Branded Food Products Database) for annotating eating …

Enhancing Innovation Via The Digital Twin, Nobuyuki Fukawa, Aric Rindfleisch

Business and Information Technology Faculty Research & Creative Works

A Growing Number of Firms Are Seeking to Leverage Emerging Technologies, Such as Artificial Intelligence (AI) and 3D Printing, to Enhance their Innovation Efforts. These Seemingly Distinct Technologies Are Currently Coalescing into an Encompassing New Technology Called the Digital Twin. This Technology Allows Innovative Firms to Create a Digital Replica of a Physical Entity that Evolves over its Life Cycle. This Article Explores the Implications of the Digital Twin for Innovation Theory and Practice. First, We Examine the Connection between the Digital Twin and Three Related Technologies (I.e., 3D Printing, Big Data, and AI). Second, We Create a Typology of …

Functional And Technical Outcomes In Acute Ischemic Stroke Patients With Hyperdense Middle Cerebral Artery Sign Treated With Endovascular Thrombectomy, Yimin Chen, Francesco Diana, Mohammad Mofatteh, Sijie Zhou, Juanmei Chen, Zhou Huang, Weijuan Wu, Yajie Yang, Zhiyi Zeng, Weijian Zhang, Ziqi Ouyang, Thanh N Nguyen, Shuiquan Yang, José Fidel Baizabal-Carvallo, Xuxing Liao

Children’s Nutrition Research Center Staff Publications

Background and objective: The hyperdense middle cerebral artery sign (HMCAS) is observed in a proportion of patients with acute ischemic stroke (AIS). This sign reflects the presence of an intravascular thrombus rich in red blood cells. Several studies have demonstrated that HMCAS increases the risk of poor outcomes in AIS patients treated with IV thrombolysis or no reperfusion therapy; however, whether HMCAS predicts a poor outcome in patients treated with endovascular thrombectomy (EVT) is less clear. We aimed to evaluate the functional outcome by the modified Rankin scale (mRS) at 90 days and technical challenges in patients with HMCAS undergoing …

Jump To Platform Faster? Gender, Institutional Change, And Pre-Entrant Entrepreneurial Attempt, Lei Xu, K. Praveen Parboteeah, Chevy-Hanqing Fang

Business and Information Technology Faculty Research & Creative Works

Purpose: The authors enrich and extend the existing institutional anomie theory (IAT) in the hope of sharpening the understanding of the joint effects of selected cultural values and social institutional changes on women's pre-entrant entrepreneurial attempts. The authors theorize that women are culturally discouraged to pursue pre-entrant entrepreneurial attempts or wealth accumulation in a specific culture. This discouragement creates an anomic strain that motivates women to deviate from cultural prescriptions by engaging in pre-entrant entrepreneurial attempts at a faster speed. Building on this premise, the authors hypothesize that changes in social institutions facilitate the means of achievement for women due …

3d-Printed Hydrogels Dressings With Bioactive Borate Glass For Continuous Hydration And Treatment Of Second-Degree Burns, Fateme Fayyazbakhsh, Michael J. Khayat, Candy Sadler, Delbert Day, Yue-Wern Huang, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

Recent advances in additive manufacturing have led to the development of innovative solutions for tissue regeneration. Hydrogel materials have gained significant attention for burn wound treatment in clinical practice among various advanced dressings due to their soothing and moisturizing activity. However, prolonged healing, pain, and traumatic removal due to the lack of long-term wound hydration are some of the challenges in the treatment of second-degree burn wounds. In this study, 3D-printed dressings were fabricated using gelatin, alginate, and bioactive borate glass (BBG) using an extrusion-based bioprinter. After ionic crosslinking, the 3D-printed dressings were characterized for mechanical properties, degradation rate, hydration …

Study Of Legionella Pneumophila Treatment With Copper In Drinking Water By Single Cell-Icp-Ms, Lei Xu, Austin Sigler, Anna Chernatynskaya, Lindsey Rasmussen, Jingrang Lu, Endalkachew Sahle-Demessie, David J. Westenberg, Hu Yang, Honglan Shi

Biological Sciences Faculty Research & Creative Works

Legionella pneumophila is a persistent opportunistic pathogen that poses a significant threat to domestic water systems. Previous studies suggest that copper (Cu) is an effective antimicrobial in water systems. A rapid and sensitive quantification method is desired to optimize the conditions of L. pneumophila treatment by Cu and to better understand the interaction mechanisms between Cu and cells. In this study, we developed a highly sensitive single cell (SC)-ICP-MS method to monitor L. pneumophila cell concentration and track their uptake of Cu. The SC-ICP-MS method showed excellent sensitivity (with a cell concentration detection limit of 1000 cells/mL), accuracy (good agreement …

Diffusion Basis Spectrum Imaging Detects Subclinical Traumatic Optic Neuropathy In A Closed-Head Impact Mouse Model Of Traumatic Brain Injury, Hsin-Chieh Yang, Raj Swaroop Lavadi, Andrew D Sauerbeck, Michael Wallendorf, Terrance T Kummer, Sheng-Kwei Song, Tsen-Hsuan Lin

2020-Current year OA Pubs

INTRODUCTION: Traumatic optic neuropathy (TON) is the optic nerve injury secondary to brain trauma leading to visual impairment and vision loss. Current clinical visual function assessments often fail to detect TON due to slow disease progression and clinically silent lesions resulting in potentially delayed or missed treatment in patients with traumatic brain injury (TBI).

METHODS: Diffusion basis spectrum imaging (DBSI) is a novel imaging modality that can potentially fill this diagnostic gap. Twenty-two, 16-week-old, male mice were equally divided into a sham or TBI (induced by moderate Closed-Head Impact Model of Engineered Rotational Acceleration device) group. Briefly, mice were anesthetized …

Machine Learning Pipelines Developed For The Prediction Of Cancelation Of Inappropriate Parathyroid Hormone-Related Peptide Orders Demonstrate Poor Performance In Predicting Provider Behavior, Nicholas C. Spies, Christopher W. Farnsworth, Ronald Jackups, Mark A. Zaydman

2020-Current year OA Pubs

No abstract provided.

Habenular Molecular Targets For Depression, Impulsivity, And Addiction, Snigdha Srivastava, Benjamin R Arenkiel, Ramiro Salas

Faculty, Staff and Students Publications

No abstract provided.