Digital Commons Network^™

Material Considerations For Cell & Gene Therapy Products, Ekta Mahajan, Ulrike Strauss

Single Use Technologies VI: Established, Emergent, Agile, Sustainable?

Please click Additional Files below to see the full abstract

3d Printing Technology: Supply Chain Independent Single-Use Plastic Ware And Bioreactors For Cell Culture, Lena Achleitner, Peter Satzer

Single Use Technologies VI: Established, Emergent, Agile, Sustainable?

Please click Additional Files below to see the full abstract

The Effects Of Inhaled Anesthetic Agents On Long-Term Cognitive Decline, Viktor Kunder, Matthew Tayem, Sneh Parekh

HPD Articles

Background: Anesthesia is generally considered safe, but studies have shown the potential relationship between anesthetic agents and dementia after surgery. Several studies have pointed out how the commonly used anesthetic drugs induce cytotoxicity, ultimately leading to neurodegeneration and even Alzheimer's Disease (AD). Specifically, inhaled anesthetics such as isoflurane, sevoflurane, and desflurane have been shown to impact the neurophysiology of the brain. However, there is no established relationship between aesthetics and long-term postoperative cognitive decline.

Objective: The objective of this study is to explore the available research on anesthetic agents and the potential role that they have played in postoperative cognitive …

Emerging Trends In Single Use Technology For Manufacturing Of Antibody- Drug Conjugates, Asmita Pawar

Single Use Technologies VI: Established, Emergent, Agile, Sustainable?

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Poly(Propylene Glycol)-Based Non-Isocyanate Polyurethane Ionenes: Thermal, Morphological And Conductive Properties, Jordan C. Pierce, George M. Timmermann, Creston Singer, David Salas-De La Cruz, Kevin Miller

Faculty & Staff Research and Creative Activity

The synthesis and characterization of a series of polyurethane ionenes using a non-isocyanate approach is disclosed. Imidazole-capped, urethane-containing prepolymers are prepared by first reacting carbonyl diimidazole (CDI) with several poly(propylene glycol) (PPG) diols with variable molecular weight, followed by subsequent reaction with 3-aminopropylimidazole (API). Polymerization with 1,4-dibromomethylbenzene followed by anion exchange resulted in the desired polyurethane ionenes bearing the [NTf2] counteranion as a series of viscous liquids. NMR and FTIR spectroscopy are used to characterize the intermediates and final ionenes, including molecular weight determination by end-group analysis. A single glass transition temperature (Tg), as determined by differential scanning …

The Effects Of Biological Sex And Oral Contraception On The Sympathetic Neurocirculatory Adjustments To Static Handgrip Exercise In Humans., Andrew W D'Souza, Sarah L Hissen, Kazumasa Manabe, Takuro Washio, Meghan C Annis, Belinda Sanchez, Qi Fu, Kevin Shoemaker

Kinesiology Publications

Biological sex affects the neurocirculatory adjustments to fatiguing exercise. However, the effects of sex and oral contraceptive pills (OCPs) on sympathetic action potential (AP) emission patterns and the transduction of AP discharge into vasoconstriction during static handgrip (SHG) exercise remains unknown. We tested the hypothesis that males, and females using OCPs, would demonstrate larger increases in sympathetic activation and sympathetic vascular transduction compared to naturally menstruating females during SHG and post-exercise circulatory occlusion (PECO). Young males (n=14, 25[5] yr), females using OCPs (n=16, 24[6] yr), and naturally menstruating females (n=18, 26[4] yr) underwent assessments of multi-unit muscle sympathetic nerve activity …

College Faculty In The News: Sept. 11, 2023, University Of Dayton

College of Arts and Sciences Newsroom

News agencies across the region, the nation and throughout the world often reach out to our faculty experts for their perspectives on today's issues.

The Rider (2023-09-11), Natalie Lapsley

The Rider Archives

Vol.9 No.3

The Minutes Of The Marshall University Graduate Council Meeting, August 25, 2023, Marshall University Graduate Council

Graduate Council Minutes

No abstract provided.

Stroke Survivors' And Informal Caregivers' Perceptions Of Depressive Symptoms After Stroke: A Mixed-Methods Sequential Explanatory Study, Tanya L. Breckenridge-Trotter

Dissertations

Poststroke depression (PSD) is a frequently occurring complication of stroke that affects up to 30% of all stroke survivors in the first 24 months after stroke. PSD has been correlated with increases in hospital length of stay and hospital costs, increased physical morbidity and mortality, the risk for recurrent stroke at one year, decreased functional outcome, decreased cognitive function, and decreased quality of life post stroke. Estimates are >50% stroke survivors with depressive symptoms are left untreated due to lack of recognition. Moreover, depression in family caregivers of stroke survivors may negatively influence stroke recovery and stroke survivor quality of …

Impact Of A Tlr9 Agonist And Broadly Neutralizing Antibodies On Hiv-1 Persistence: The Randomized Phase 2a Titan Trial, Jesper D. Gunst, Jesper Falkesgaard Højen, Marie H. Pahus, Miriam Rosás-Umbert, Birgitte Stiksrud, James Mcmahon, Paul Denton, Henrik Nielsen, Isik S. Johansen, Thomas Benfield, Steffen Leth, Jan Gerstoft, Lars Østergaard, Mariane H. Schleimann, Rikke Olesen, Henrik Støvring, Line K. Vibholm, Nia Weis, Anne M. Dyrhol-Riise, Karen B.H. Pedersen, Jillian S.Y. Lau, Dennis C. Copertino, Noemi Linden, Tan T. Huynh, Victor Ramos, Rl Brad Jones, Sharon R. Lewin, Martin Tolstrup, Thomas A. Rasmussen, Michel C. Nussenzweig, Marina Caskey, Dag Henrik Reikvam, Ole Schmeltz Søgaard

Biology Faculty Publications

Inducing antiretroviral therapy (ART)-free virological control is a critical step toward a human immunodeficiency virus type 1 (HIV-1) cure. In this phase 2a, placebo-controlled, double-blinded trial, 43 people (85% males) with HIV-1 on ART were randomized to (1) placebo/placebo, (2) lefitolimod (TLR9 agonist)/placebo, (3) placebo/broadly neutralizing anti-HIV-1 antibodies (bNAbs) or (4) lefitolimod/bNAb. ART interruption (ATI) started at week 3. Lefitolimod was administered once weekly for the first 8 weeks, and bNAbs were administered twice, 1 d before and 3 weeks after ATI. The primary endpoint was time to loss of virologic control after ATI. The median delay in time to …

Dfcp1 Regulates Lipid Droplet Catabolism In An Ntpase-Dependent Manner, Victoria Anne Ismail

Arts & Sciences Graduate Student Theses and Dissertations

Lipid Droplets (LDs) are lipid storage organelles that can be readily tapped to resupply cells with energy or lipid building blocks, and, therefore, play a central role in cellular metabolism. It has emerged that Double FYVE Domain Containing Protein 1 (DFCP1), which has routinely been used as a marker for early autophagy, is an LD-associated protein and is implicated in establishing contacts between the endoplasmic reticulum (ER) and LDs, but little is known about DFCP1’s function or how it influences either autophagy or LD metabolism. Here, we show that DFCP1 contains a novel NTPase domain that regulates its assembly onto …

Drives Blunted By Time: The Effects Of Aging On Motivational Behaviors And Responses To Social Isolation In Mice, Hanyue Lei

Arts & Sciences Graduate Student Theses and Dissertations

Mental health in the elderly is an escalating social problem as the world population ages rapidly. Older adults are more susceptible to stressors like social isolation, which is a common risk factor for many mental disorders and health issues. However, how aging affects specific mental functions like motivational drive and stress responses, as well as the underlying neurobiological mechanisms, remains largely unstudied. We comprehensively examined behavioral phenotypes in both young and aged mice under the normal grouped-housing condition versus chronic social isolation treatment. The results suggested that natural aging and social isolation both impaired motivational behaviors and induce depressive- and …

De Novo Transcriptome Analysis Of Solanum Lycopersicum Cv. Super Strain B Under Drought Stress, Hassan S. Al-Zahrani, Tarek A.A. Moussa, Hameed Alsamadany, Rehab M. Hafez, Michael P. Fuller

School of Biological and Marine Sciences

Tomato cv. super strain B was widely cultivated in Saudi Arabia under drought stress. Illumina Hiseq-2000 was used to create the transcriptional profile of tomato cultivar super strain B. A total of 98,069 contigs were gathered, with an average length of 766 bp. Most of the genes in the gene ontology (GO) analysis were categorized into molecular function (MF) of ATP binding (1301 genes), metal ion binding (456 genes), protein kinase activity (392 genes), transferase activity (299 genes), Biological process (BP) of DNA-templated genes (366 genes), and regulation of transcription genes (209 genes), while cellular components (CC) of integral component …

The Minutes Of The Marshall University Graduate Council Meeting, August 25, 2023, Marshall University Graduate Council

Graduate Council Minutes

No abstract provided.

The Role Of Gardnerella In Bacterial Vaginosis And Facilitating Colonization By Neisseria Gonorrhoeae, Sydney Rose Morrill

Arts & Sciences Graduate Student Theses and Dissertations

Bacterial vaginosis (BV) is a condition of vaginal flora that affects about 1/3 of reproductive age women. The optimal vaginal microbiome is thought to be one dominated by Lactobacillus species. In the BV-positive microbiome, this lactobacillus-dominance is replaced by an outgrowth of diverse anaerobic bacteria. BV has been clinically linked to a number of negative health outcomes, including preterm birth, infertility, cervical dysplasia, and an increased risk of sexually-transmitted infections (STIs) such as gonorrhea. The underlying cause(s) of BV have been debated in the literature for over half a century. In 1955, it was first reported that a bacterium now …

Collagene Enables Privacy-Aware Federated And Collaborative Genomic Data Analysis, Wentao Li, Miran Kim, Kai Zhang, Han Chen, Xiaoqian Jiang, Arif Harmanci

Faculty, Staff and Student Publications

Growing regulatory requirements set barriers around genetic data sharing and collaborations. Moreover, existing privacy-aware paradigms are challenging to deploy in collaborative settings. We present COLLAGENE, a tool base for building secure collaborative genomic data analysis methods. COLLAGENE protects data using shared-key homomorphic encryption and combines encryption with multiparty strategies for efficient privacy-aware collaborative method development. COLLAGENE provides ready-to-run tools for encryption/decryption, matrix processing, and network transfers, which can be immediately integrated into existing pipelines. We demonstrate the usage of COLLAGENE by building a practical federated GWAS protocol for binary phenotypes and a secure meta-analysis protocol. COLLAGENE is available at https://zenodo.org/record/8125935 …

Global Proteomic Identifies Multiple Cancer-Related Signaling Pathways Altered By A Gut Pathobiont Associated With Colorectal Cancer, Ewa Pasquereau-Kotula, Giulia Nigro, Florent Dingli, Damarys Loew, Patrick Poullet, Yi Xu, Scott Kopetz, Jennifer Davis, Lucie Peduto, Catherine Robbe-Masselot, Philippe Sansonetti, Patrick Trieu-Cuot, Shaynoor Dramsi

Faculty, Staff and Student Publications

In this work, we investigated the oncogenic role of Streptococcus gallolyticus subsp. gallolyticus (SGG), a gut bacterium associated with colorectal cancer (CRC). We showed that SGG UCN34 accelerates colon tumor development in a chemically induced CRC murine model. Full proteome and phosphoproteome analysis of murine colons chronically colonized by SGG UCN34 revealed that 164 proteins and 725 phosphorylation sites were differentially regulated. Ingenuity Pathway Analysis (IPA) indicates a pro-tumoral shift specifically induced by SGG UCN34, as ~ 90% of proteins and phosphoproteins identified were associated with digestive cancer. Comprehensive analysis of the altered phosphoproteins using ROMA software revealed up-regulation of …

Elimination Of Oncogenic Kras In Genetic Mouse Models Eradicates Pancreatic Cancer By Inducing Fas-Dependent Apoptosis By Cd8+ T Cells, Krishnan K Mahadevan, Valerie S Lebleu, Elena V Ramirez, Yang Chen, Bingrui Li, Amari M Sockwell, Mihai Gagea, Hikaru Sugimoto, Lakshmi Kavitha Sthanam, Desiree Tampe, Michael Zeisberg, Haoqiang Ying, Abhinav K Jain, Ronald A Depinho, Anirban Maitra, Kathleen M Mcandrews, Raghu Kalluri

Faculty, Staff and Student Publications

Oncogenic KRASG12D (KRAS∗) is critical for the initiation and maintenance of pancreatic ductal adenocarcinoma (PDAC) and is a known repressor of tumor immunity. Conditional elimination of KRAS∗ in genetic mouse models of PDAC leads to the reactivation of FAS, CD8+ T cell-mediated apoptosis, and complete eradication of tumors. KRAS∗ elimination recruits activated CD4+ and CD8+ T cells and promotes the activation of antigen-presenting cells. Mechanistically, KRAS∗-mediated immune evasion involves the epigenetic regulation of Fas death receptor in cancer cells, via methylation of its promoter region. Furthermore, analysis of human RNA sequencing identifies that high KRAS expression in PDAC tumors shows …

Unfolding The Secrets Of Small Cell Lung Cancer Progression: Novel Approaches And Insights Through Rapid Autopsies, Zsolt Megyesfalvi, Simon Heeke, Benjamin J Drapkin, Anna Solta, Ildiko Kovacs, Kristiina Boettiger, Lilla Horvath, Busra Ernhofer, Janos Fillinger, Ferenc Renyi-Vamos, Clemens Aigner, Karin Schelch, Christian Lang, Gyorgy Marko-Varga, Carl M Gay, Lauren A Byers, Benjamin B Morris, John V Heymach, Peter Van Loo, Fred R Hirsch, Balazs Dome

Faculty, Staff and Student Publications

The understanding of small cell lung cancer (SCLC) biology has increased dramatically in recent years, but the processes that allow SCLC to progress rapidly remain poorly understood. Here, we advocate the integration of rapid autopsies and preclinical models into SCLC research as a comprehensive strategy with the potential to revolutionize current treatment paradigms.

Early Onset Horizontal Gaze Palsy And Progressive Scoliosis Due To A Noncanonical Splicing-Site Variant And A Missense Variant In The Robo3 Gene, Sheng Yi, Zailong Qin, Xunzhao Zhou, Junjie Chen, Shang Yi, Qiuli Chen, Limei Huang, Qinle Zhang, Biyan Chen, Jingsi Luo

Faculty, Staff and Student Publications

BACKGROUND: Homozygous or compound heterozygous ROBO3 gene mutations cause horizontal gaze palsy with progressive scoliosis (HGPPS). This is an autosomal recessive disorder that is characterized by congenital absence or severe restriction of horizontal gaze and progressive scoliosis. To date, almost 100 patients with HGPPS have been reported and 55 ROBO3 mutations have been identified.

METHODS: We described an HGPPS patient and performed whole-exome sequencing (WES) to identify the causative gene.

RESULTS: We identified a missense variant and a splice-site variant in the ROBO3 gene in the proband. Sanger sequencing of cDNA revealed the presence of an aberrant transcript with retention …

Proteogenomic Insights Suggest Druggable Pathways In Endometrial Carcinoma, Yongchao Dou, Lizabeth Katsnelson, Marina A Gritsenko, Yingwei Hu, Boris Reva, Runyu Hong, Yi-Ting Wang, Iga Kolodziejczak, Rita Jui-Hsien Lu, Chia-Feng Tsai, Wen Bu, Wenke Liu, Xiaofang Guo, Eunkyung An, Rebecca C Arend, Jasmin Bavarva, Lijun Chen, Rosalie K Chu, Andrzej Czekański, Teresa Davoli, Elizabeth G Demicco, Deborah Delair, Kelly Devereaux, Saravana M Dhanasekaran, Peter Dottino, Bailee Dover, Thomas L Fillmore, Mckenzie Foxall, Catherine E Hermann, Tara Hiltke, Galen Hostetter, Marcin Jędryka, Scott D Jewell, Isabelle Johnson, Andrea G Kahn, Amy T Ku, Chandan Kumar-Sinha, Paweł Kurzawa, Alexander J Lazar, Rossana Lazcano, Jonathan T Lei, Yi Li, Yuxing Liao, Tung-Shing M Lih, Tai-Tu Lin, John A Martignetti, Ramya P Masand, Rafał Matkowski, Wilson Mckerrow, Mehdi Mesri, Matthew E Monroe, Jamie Moon, Ronald J Moore, Michael D Nestor, Chelsea Newton, Tatiana Omelchenko, Gilbert S Omenn, Samuel H Payne, Vladislav A Petyuk, Ana I Robles, Henry Rodriguez, Kelly V Ruggles, Dmitry Rykunov, Sara R Savage, Athena A Schepmoes, Tujin Shi, Zhiao Shi, Jimin Tan, Mason Taylor, Mathangi Thiagarajan, Joshua M Wang, Karl K Weitz, Bo Wen, C M Williams, Yige Wu, Matthew A Wyczalkowski, Xinpei Yi, Xu Zhang, Rui Zhao, David Mutch, Arul M Chinnaiyan, Richard D Smith, Alexey I Nesvizhskii, Pei Wang, Maciej Wiznerowicz, Li Ding, D R Mani, Hui Zhang, Matthew L Anderson, Karin D Rodland, Bing Zhang, Tao Liu, David Fenyö

Faculty, Staff and Students Publications

We characterized a prospective endometrial carcinoma (EC) cohort containing 138 tumors and 20 enriched normal tissues using 10 different omics platforms. Targeted quantitation of two peptides can predict antigen processing and presentation machinery activity, and may inform patient selection for immunotherapy. Association analysis between MYC activity and metformin treatment in both patients and cell lines suggests a potential role for metformin treatment in non-diabetic patients with elevated MYC activity. PIK3R1 in-frame indels are associated with elevated AKT phosphorylation and increased sensitivity to AKT inhibitors. CTNNB1 hotspot mutations are concentrated near phosphorylation sites mediating pS45-induced degradation of β-catenin, which may render …

Reduced Maternal Immunity And Vertical Transfer Of Immunity Against Sars-Cov-2 Variants Of Concern With Covid-19 Exposure Or Initial Vaccination In Pregnancy., Rupsa Boelig, Sidhartha Chaudhury, Gregory D Gromowski, Sandra Mayer, Jocelyn King, Zubair H Aghai, Elke Bergmann-Leitner

Department of Obstetrics and Gynecology Faculty Papers

INTRODUCTION: As the SARS-CoV-2 pandemic continues to evolve, we face new variants of concern with a concurrent decline in vaccine booster uptake. We aimed to evaluate the difference in immunity gained from the original SARS-CoV-2 mRNA vaccine series in pregnancy versus SARS-CoV-2 exposure during pregnancy against recent variants of concern.

STUDY DESIGN: This is a retrospective analysis of previously collected samples from 192 patients who delivered between February 2021 and August 2021. Participants were categorized as 1) COVID vaccine: mRNA vaccine in pregnancy, 2) COVID-exposed, and 3) controls. The primary outcome was neutralizing capacity against wild-type, Delta, and Omicron-B1 between …

John Cupples Oral History Interview, John Cupples

Raymond College

No abstract provided.

Stomatal Opening Efficiency Is Controlled By Cell Wall Organization In Arabidopsis Thaliana, Sedighe Keynia, Leila Jaafar, You Zhou, Charles T. Anderson, Joseph A. Turner

Department of Mechanical and Materials Engineering: Faculty Publications

Stomatal function in plants is regulated by the nanoscale architecture of the cell wall and turgor pressure, which together control stomatal pore size to facilitate gas exchange and photosynthesis. The mechanical properties of the cell wall and cell geometry are critical determinants of stomatal dynamics. However, the specific biomechanical functions of wall constituents, for example, cellulose and pectins, and their impact on the work required to open or close the stomatal pore are unclear. Here, we use nanoindentation in normal and lateral directions, computational modeling, and microscopic imaging of cells from the model plant Arabidopsis thaliana to investigate the precise …

Preface: Causes Of Obesity, Theories, Conjectures And Evidence, David B Allison, Thorkild I A Sørensen, Kevin D Hall, John R Speakman

Children’s Nutrition Research Center Staff Publications

No abstract provided.

Intouch Week Of September 11, 2023, New York Medical College

InTouch

• Intranasal Immunization Offers Stronger Protection Against COVID-19
• Campus Playground Gets a Makeover
• SOM Student Publishes Research Conducted In Prestigious Fellowship Program
• NYMC and TU Hosts “Ninety Minutes: The Medical Magazine of the Web”
• Calling Artists For The Asprinio Fitness Center Wall Project
• NYMC Research: Patric K. Stanton, Ph.D.

Treatment Of Older Adults With Flt3-Mutated Aml: Emerging Paradigms And The Role Of Frontline Flt3 Inhibitors, Nicholas J Short, Daniel Nguyen, Farhad Ravandi

Faculty, Staff and Student Publications

FLT3 is the most frequently mutated gene in acute myeloid leukemia (AML), with FLT3 internal tandem duplication (ITD) mutations being associated with a more aggressive clinical course. While two large, randomized clinical trials have shown a survival benefit with the frontline use of an oral FLT3 inhibitor (midostaurin or quizartinib) in patients with FLT3-mutated AML, the role of FLT3 inhibitors in older adults with newly diagnosed FLT3-mutated AML remains unclear. A definitive improvement in survival has not been observed in intensively treated patients over 60 years of age receiving frontline FLT3 inhibitors. Furthermore, many patients with FLT3-mutated AML are unsuitable …

Butte Priority Soils Operable Unit (Bpsou) Final Insufficiently Reclaimed (Ir) Sites Field Sampling And Investigation Plan (Fsp) Butte Remediation Evaluation System (Bres) No.174 – Buffalo Ditch, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Butte Priority Soils Operable Unit (Bpsou) Final Insufficiently Reclaimed (Ir) Sites Field Sampling Plan (Fsp) Bres No. 48 – Old Glory West, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.