Digital Commons Network^™

Allostatic Load Index Effectively Measures Chronic Stress Status In Zoo-Housed Giraffes, Haley N. Beer, Lisa K. Karr, Trenton C. Schrader, Dustin T. Yates

Department of Animal Science: Faculty Publications

For giraffes, few standardized methods exist for quantifying chronic stress. Allostatic load index is quantified from a panel of multi-system stress biomarkers to estimate cumulative stress. Our objective was to determine whether a panel of biomarkers selected for their role in allostatic load would reflect the number of documented stress events experienced by giraffes. Cortisol, DHEA-S, cholesterol, non-esterified fatty acids (NEFA), and fructosamine were determined in serum samples from zoo-housed giraffes (n = 18). These were correlated with the overall number and frequency of ZIMS-documented stress events experienced prior to blood collection. We also compared giraffes grouped by high vs. …

Neutron Dark-Field Imaging Of Hydrogen-Fatigued Pressure Vessel Steel, Youngju Kim, Daniel S. Hussey, Katie M. Weigandt, Pushkar Sathe, Peter N. Bajcsy, Paul A Kienzle, Sarah M. Robinson, Nikolai N. Klimov, Ryan P. Murphy, Michael G. Huber, Caitlyn M. Wolf, Zachary N. Buck, Matthew J. Connolly

International Hydrogen Conference: Understanding Hydrogen-Materials Interactions

Please click Additional Files below to see the full abstract

Thyroid Predictors Of Postpartum Mood Disorders, Sean Backer, Janeta Yancheva, Camelia Garcia, Deepesh Khanna

HPD Articles

Postpartum mood disorders (PMD) are currently among the leading causes of maternal postpartum morbidity and mortality. PMD include the conditions of postpartum blues (PB), postpartum depression (PPD), and postpartum psychosis. The pathogenesis of PMDs are ambiguous, and there are no reliable prenatal predictive markers despite current research efforts. Even though reliable indicators have not been found, leading ideas suggest an etiology of hormonal fluctuations. Although thyroid markers have long been linked to psychiatric disorders such as major depressive disorder (MDD), how they correlate with PMDs is still unclear. This study aimed to evaluate the pathophysiological link between thyroid function, PMDs, …

“A More Perfect World”: Posthumanism And Technological Integration In A Memory Called Empire, Garrett R. Johnson

Masters Theses

This thesis considers the relationship of technology to the human through a posthumanist lens, questioning what will become of the human an increasingly more-than-human world through an examination of the novel A Memory Called Empire, by Arkady Martine. Through an examination of the imago device from the novel, the thesis evaluates the human experience of memory and the influence of empire. The thesis advances four key concepts: 1. The collapsed divide between the human and the technic through memory and imagination; 2. Technology’s development and use; 3. The user that is integrated with the technic; and 4. The poisonous influence …

September 19, 2023 Ereporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Methods For The Treatment Of Inflammation, Bill J. Baker, Sofia Kokkaliari, James Leahy, Wayne Caleb Williams

USF Patents

The present disclosure is directed to methods for the treatment of inflammation or pain, particularly methods using compositions containing a compound of formula (I).

The Influence Of Polystyrene Microplastics On Juvenile Steelhead Trout (Oncorhynchus Mykiss), Kaitlyn Marie Baker

Dissertations and Theses

Mass production of plastic within the past decade has led to over 100 billion tons of plastics being added to the world’s oceans through rivers and effluent disposal and decomposition. For marine environments, the sudden and constant growth of microplastics (plastics 1 µm to 5 mm in diameter), is of particular concern to top-predatory fish such as steelhead trout (Oncorhynchus mykiss), who passively or actively uptake microplastics while consuming prey. Previous research has demonstrated clear detrimental impacts of microplastic accumulation in bivalves, seabirds, and zebrafish, including decreased diet, reproduction rates, and metabolism, as well as increased rates of …

Crk And Crkl Are Required In The Endocardial Lineage For Heart Valve Development, Bingruo Wu, Brian Wu, Sonia Benkaci, Lijie Shi, Pengfei Lu, Taeju Park, Bernice E. Morrow, Yidong Wang, Bin Zhou

Manuscripts, Articles, Book Chapters and Other Papers

Background Endocardial cells are a major progenitor population that gives rise to heart valves through endocardial cushion formation by endocardial to mesenchymal transformation and the subsequent endocardial cushion remodeling. Genetic variants that affect these developmental processes can lead to congenital heart valve defects. Crk and Crkl are ubiquitously expressed genes encoding cytoplasmic adaptors essential for cell signaling. This study aims to explore the specific role of Crk and Crkl in the endocardial lineage during heart valve development. Methods and Results We deleted Crk and Crkl specifically in the endocardial lineage. The resultant heart valve morphology was evaluated by histological analysis, …

Abcc1 And Glutathione Metabolism Limit The Efficacy Of Bcl-2 Inhibitors In Acute Myeloid Leukemia, Jessica Ebner, Johannes Schmoellerl, Martin Piontek, Gabriele Manhart, Selina Troester, Bing Z Carter, Heidi Neubauer, Richard Moriggl, Gergely Szakács, Johannes Zuber, Thomas Köcher, Michael Andreeff, Wolfgang R Sperr, Peter Valent, Florian Grebien

Faculty, Staff and Student Publications

The BCL-2 inhibitor Venetoclax is a promising agent for the treatment of acute myeloid leukemia (AML). However, many patients are refractory to Venetoclax, and resistance develops quickly. ATP-binding cassette (ABC) transporters mediate chemotherapy resistance but their role in modulating the activity of targeted small-molecule inhibitors is unclear. Using CRISPR/Cas9 screening, we find that loss of ABCC1 strongly increases the sensitivity of AML cells to Venetoclax. Genetic and pharmacologic ABCC1 inactivation potentiates the anti-leukemic effects of BCL-2 inhibitors and efficiently re-sensitizes Venetoclax-resistant leukemia cells. Conversely, ABCC1 overexpression induces resistance to BCL-2 inhibitors by reducing intracellular drug levels, and high ABCC1 levels …

Mir-146a Inhibits Ovarian Tumor Growth In Vivo Via Targeting Immunosuppressive Neutrophils And Enhancing Cd8+ T Cell Infiltration, Rui Chen, Elaina Coleborn, Chintan Bhavsar, Yue Wang, Louisa Alim, Andrew N Wilkinson, Michelle A Tran, Gowri Irgam, Sharat Atluri, Kiefer Wong, Jae-Jun Shim, Siddharth Adityan, Ju-Seog Lee, Willem W Overwijk, Raymond Steptoe, Da Yang, Sherry Y Wu

Faculty, Staff and Student Publications

Immunotherapies have emerged as promising strategies for cancer treatment. However, existing immunotherapies have poor activity in high-grade serous ovarian cancer (HGSC) due to the immunosuppressive tumor microenvironment and the associated low tumoral CD8+ T cell (CTL) infiltration. Through multiple lines of evidence, including integrative analyses of human HGSC tumors, we have identified miR-146a as a master regulator of CTL infiltration in HGSC. Tumoral miR-146a expression is positively correlated with anti-cancer immune signatures in human HGSC tumors, and delivery of miR-146a to tumors resulted in significant reduction in tumor growth in both ID8-p53−/− and IG10 murine HGSC models. Increasing miR-146a expression …

A Blood-Based Metabolomic Signature Predictive Of Risk For Pancreatic Cancer, Ehsan Irajizad, Ana Kenney, Tiffany Tang, Jody Vykoukal, Ranran Wu, Eunice Murage, Jennifer B Dennison, Marta Sans, James P Long, Maureen Loftus, John A Chabot, Michael D Kluger, Fay Kastrinos, Lauren Brais, Ana Babic, Kunal Jajoo, Linda S Lee, Thomas E Clancy, Kimmie Ng, Andrea Bullock, Jeanine M Genkinger, Anirban Maitra, Kim-Anh Do, Bin Yu, Brian M Wolpin, Sam Hanash, Johannes F Fahrmann

Faculty, Staff and Student Publications

Emerging evidence implicates microbiome involvement in the development of pancreatic cancer (PaCa). Here, we investigate whether increases in circulating microbial-related metabolites associate with PaCa risk by applying metabolomics profiling to 172 sera collected within 5 years prior to PaCa diagnosis and 863 matched non-subject sera from participants in the Prostate, Lung, Colorectal, and Ovarian (PLCO) cohort. We develop a three-marker microbial-related metabolite panel to assess 5-year risk of PaCa. The addition of five non-microbial metabolites further improves 5-year risk prediction of PaCa. The combined metabolite panel complements CA19-9, and individuals with a combined metabolite panel + CA19-9 score in the …

Deep Learning Integrates Histopathology And Proteogenomics At A Pan-Cancer Level, Joshua M Wang, Runyu Hong, Elizabeth G Demicco, Jimin Tan, Rossana Lazcano, Andre L Moreira, Yize Li, Anna Calinawan, Narges Razavian, Tobias Schraink, Michael A Gillette, Gilbert S Omenn, Eunkyung An, Henry Rodriguez, Aristotelis Tsirigos, Kelly V Ruggles, Li Ding, Ana I Robles, D R Mani, Karin D Rodland, Alexander J Lazar, Wenke Liu, David Fenyö, Clinical Proteomic Tumor Analysis Consortium

Faculty, Staff and Student Publications

We introduce a pioneering approach that integrates pathology imaging with transcriptomics and proteomics to identify predictive histology features associated with critical clinical outcomes in cancer. We utilize 2,755 H&E-stained histopathological slides from 657 patients across 6 cancer types from CPTAC. Our models effectively recapitulate distinctions readily made by human pathologists: tumor vs. normal (AUROC = 0.995) and tissue-of-origin (AUROC = 0.979). We further investigate predictive power on tasks not normally performed from H&E alone, including TP53 prediction and pathologic stage. Importantly, we describe predictive morphologies not previously utilized in a clinical setting. The incorporation of transcriptomics and proteomics identifies pathway-level …

The Regulation Of Irs1 Via S6k-1 And P300 Activity, Vhuthuhawe Tintswalo Madzinge

Doctoral Dissertations and Projects

The study of IRS-1 has increased over the years due to the prevalence of cardiometabolic diseases. An increase in IRS-1 serine phosphorylation is often associated with the development of insulin resistance due to the downregulation bestowed upon it by different kinases such as p70 S6K-1. Recently, p300 has been shown to acetylate IRS-1 thus leading to its downregulation. However, studies have not looked into the relationship that exists between serine phosphorylation and lysine acetylation of IRS-1. In this study, insulin stimulated HepG2 cells were treated with either PF4708671, a selective p70 S6K-1 inhibitor, or C646, a selective p300 inhibitor. PF4708671 …

The Lived Experience Of Residential Undergraduates From Minority Ethnic Backgrounds Diagnosed With A Mental Health Condition, Cheryl Gowdie

Doctoral Dissertations and Projects

Research has demonstrated that mental health illnesses have increased, particularly among college students. Although there is a general understanding of the implications of this trend, there remains a lack of evidence on the impact of mental health diagnoses on residential undergraduate minority ethnic students. This qualitative phenomenological study aims to gain insight into the lived experiences of 10 residential undergraduate minority students aged 18 to 36 diagnosed with one or more mental health disorders. The goal is to understand their personal experiences, including any stigma they may have encountered. Through comprehensive interviews, the study explored their lived experiences. The findings …

Teachers’ Perceptions On The Influence Of Early Childhood Screenings: A Qualitative Case Study, Jennifer Youssif

Doctoral Dissertations and Projects

The purpose of this qualitative case study was to examine the perceptions of teachers' experiences with the Early Childhood Screening for students attending XYZ School in Eastern Saudi Arabia. The study was guided by the theory of social constructivism and the theory of self-regulation, as they explain how early experiences influence child and adult development. The central research question addressed the experiences of early childhood educators implementing mandatory screening assessments in Pre-K and Kindergarten. The central research question was “What are the experiences of early childhood educators that implement mandatory screening assessments in Pre-K and Kindergarten?” The study followed a …

Returning To School After Covid-19: Relationships Between Parental Stress And Resilience With Children’S Mental Health Behaviors, Christa P. Stanley

Doctoral Dissertations and Projects

During the COVID-19 lockdowns, experts predicted an increase of children’s mental health issues. However, not all children showed symptoms after lockdowns lifted. The study’s purpose was to examine parental stress and resilience during COVID-19 lockdowns and their relationship to children’s behaviors, specifically anxiety and aggression after lockdown. Participants included 61 parents of children currently aged 7-11 who did not have mental health disorders before COVID lockdowns. Most parents were white, non-Hispanic within middle to high income ranges. The quantitative study used a correlational design. Parents were recruited through school websites and social media sites which linked them to a 122-question …

A Phenomenological Study Of Teacher Experiences With Implementation Of Crisis Prevention And Intervention In Military-Connected Schools, Rebecca Young Carmichael

Doctoral Dissertations and Projects

The purpose of this transcendental, phenomenological study was to describe the lived experiences of educators with classroom-based crises at military-connected schools and the crisis prevention and intervention training they have received. Knowles’ theory on adult education guided this study as it provides a useful framework for evaluating the provision and implementation of effective staff training. This qualitative study followed a phenomenological research design and involved 10 special education and general education teachers from military-connected schools. Sources of data included interviews, letters to new teachers, and a focus group. Data gathered was analyzed using the procedures of phenomenological reduction, imaginative variation, …

Sepepquant Enhances The Detection Of Possible Isoform Regulations In Shotgun Proteomics, Yongchao Dou, Yuejia Liu, Xinpei Yi, Lindsey K Olsen, Hongwen Zhu, Qiang Gao, Hu Zhou, Bing Zhang

Faculty, Staff and Students Publications

Shotgun proteomics is essential for protein identification and quantification in biomedical research, but protein isoform characterization is challenging due to the extensive number of peptides shared across proteins, hindering our understanding of protein isoform regulation and their roles in normal and disease biology. We systematically assess the challenge and opportunities of shotgun proteomics-based protein isoform characterization using in silico and experimental data, and then present SEPepQuant, a graph theory-based approach to maximize isoform characterization. Using published data from one induced pluripotent stem cell study and two human hepatocellular carcinoma studies, we demonstrate the ability of SEPepQuant in addressing the key …

Accuracy-Based Glomerular Filtration Rate Assessment By Plasma Iohexol Clearance In Kidney Transplant Donors, Zhicheng Jin, Rongrong Huang, Paul Christensen, Roger L Bertholf, Xin Yi

Faculty, Staff and Students Publications

Background: An accurate measurement of the glomerular filtration rate (GFR) is essential for detecting renal insufficiency in living kidney donors. Iohexol is a "near-ideal" exogenous filtration marker for GFR measurements that has attracted increasing interest in clinical practice because it is non-toxic, non-radioactive, readily available, and easy to measure. In this study, we aimed to set up a laboratory test to conveniently assess the plasma clearance of iohexol in living kidney donors.

Methods: A workflow was established in the institution's infusion clinic to administer iohexol and to collect three timed blood samples from renal transplant donors. Iohexol was thereafter measured …

Cognitive Machine Individualism In A Symbiotic Cybersecurity Policy Framework For The Preservation Of Internet Of Things Integrity: A Quantitative Study, Gary C. Wong

Doctoral Dissertations and Projects

This quantitative study examined the complex nature of modern cyber threats to propose the establishment of cyber as an interdisciplinary field of public policy initiated through the creation of a symbiotic cybersecurity policy framework. For the public good (and maintaining ideological balance), there must be recognition that public policies are at a transition point where the digital public square is a tangible reality that is more than a collection of technological widgets. The academic contribution of this research project is the fusion of humanistic principles with Internet of Things (IoT) technologies that alters our perception of the machine from an …

Pedophilia: Understanding The Origins And Problems Within The Criminal Justice System, James K. Mcelhaney

Doctoral Dissertations and Projects

Societal stigma, criminal sanctions, and the mental illness or disorder of pedophilia are explored in this paper because of the considerable research gaps that have accumulated over the past decade. Most of the data acquired regarding individuals with pedophilia have been predominantly from male offenders, with most of the information coming from reports to local law enforcement and the FBI. The outcome of multiple studies shows that neuroimaging, associated brainwaves, and related sexual attraction are significantly different for individuals with pedophiliac tendencies than for others. Studies also reveal that research has been limited to prevention techniques and therapy for nonoffenders …

Exploring Resiliency To Burnout: A Phenomenological Study Of Addiction Counselors Resilient To Burnout, Brian Paulson

Doctoral Dissertations and Projects

This transcendental phenomenological study aimed to describe the lived experiences of addiction counselors who appeared resilient to burnout (BO) and compassion fatigue (CF). The research question to frame this study was: What are the lived experiences of addiction counselors who appear resilient to severe levels of burnout and compassion fatigue, leading to longevity in the addiction counseling field? This included resiliency factors to resolve BO and CF, coping skills to resolve symptoms of BO and CF, and internal and external factors that contributed to BO and CF. Several theories were used to conduct this study, including Social Cognitive Theory, Social …

Elementary Music Teacher Preparation Gaps In Cultural Diversity: Exceptional Populations, Joshua Learn

Doctoral Dissertations and Projects

Despite the extensive coursework and training in teacher preparation programs focusing on diverse populations, many teachers feel underprepared to meet the needs of emerging subcultures in the elementary music classroom. Over the years, the term diversity has grown beyond ethnicity and requires continued study and attention. This qualitative study explores the perspectives of elementary music educators about diverse populations of exceptional students with Other Health Impairments (OHI), defined as asthma, ADD, ADHD, diabetes, epilepsy, heart condition, hemophilia, lead poisoning, leukemia, nephritis, rheumatic fever, sickle cell anemia, and Tourette syndrome, in their classrooms. The results of this study identify the existing …

Spiritual Formation: A Church-Based Solution To The Problem Of Disconnected African American Men In The Community, Pamela M. Phillips

Doctoral Dissertations and Projects

African American men are among the most disconnected people groups in this country due to the many disparities that have served to marginalize them out of some aspects of society. Specifically, the disparity of fatherlessness has played a major role in the disconnect of African men from the church and the community. Through this project a curriculum on the Fatherhood of God will be offered through the Christian Education department at Word of Truth International Life Center in Winston-Salem, North Carolina. Spiritual formation for African American men reconnects them to God and opens the door for reconnections to families, churches, …

Influence Of Gifted Instructional Strategies On Gifted Children’S Perceptual Reasoning: A Quasi-Experimental, Nonequivalent Group Comparison Study, Dafne Ashley Almazán

Doctoral Dissertations and Projects

The purpose of this quantitative, quasi-experimental nonequivalent group research was to analyze hypothesized differences in gifted students’ perceptual reasoning based on the type of instructional strategy they receive. Specifically, this study examined possible statistically significant differences among gifted students’ reasoning after receiving at least three years of full-time differentiated instruction, cluster grouping, or general education-focused teaching. Comparing gifted students’ perceptual reasoning and analyzing the impact of gifted instructional strategies and general systems will influence improvements in effective strategies for teaching gifted children. The study used the subscale of Perceptual Reasoning of the Wechsler Intelligence Scale for Children (WISC-IV) to examine …

The Effects Of Suicide Exposure In Adolescents: Absences, Academic Performance, And Behavior, Mika Pearson Brockington

Doctoral Dissertations and Projects

This quantitative research study is primarily about suicide exposure to adolescents and its effects. Most articles do not address the effects of exposure but only the ideation, attempts, and completion of suicide. In addition to the literature examined is the proposal to do more research to assist counselors and school staff in identifying and addressing students’ problems with academic progress, absences, and behavior. This article asked questions on a survey of participants exposed to suicide during their adolescent years. This information will be captured and analyzed to understand better how being exposed to suicide can affect many aspects of life …

A Phenomenological Study Of The Relationship Experiences Of Partners Of Individuals Who Suffer With Premenstrual Dysphoric Disorder (Pmdd), Rose Anna Alkattan

Doctoral Dissertations and Projects

Premenstrual Dysphoric Disorder (PMDD) is a debilitating disorder that adversely affects the lives of individuals and their intimate relationships. The purpose of this transcendental phenomenological study was to describe the lived experience of the partners of individuals who suffer from Premenstrual Dysphoric Disorder. The theories guiding this study were the interpersonal theory of suicide and the adult attachment theory. This study examined the overall relationship experience of partners of individuals suffering from PMDD and their cyclic attachment styles during the luteal and follicular phases of the menstrual cycle. A selection of three PMDD partners and three PMDD sufferers, who have …

Motivation Within The Inclusive Art Classroom: A Phenomenology On Engaging Students With Emotional And Behavioral Disorders, Christina Marie Cox

Doctoral Dissertations and Projects

The purpose of this transcendental phenomenological study was to describe the experiences of K-12 art teachers motivating students with emotional and behavioral disorders within the Central and Eastern Regions of the United States. The theory guiding this study is Ryan and Deci’s self-determination theory which guides the understanding that students with emotional and behavioral disorders whose psychological well-being is supported by their art educators may develop higher motivation and reach their full creative potential. As this study was designed to describe the lived experiences of K-12 art educators who have enhanced motivation in students with emotional and behavioral disorders, the …

Hypothermia In The Operating Room Promoting Normothermia In The Hysterectomy Population, Adina Indries

Doctoral Dissertations and Projects

Certified Registered Nurse Anesthetists are Advanced Practice Providers that provide safe, evidence-based practice to their surgical patients. They not only monitor the vital signs throughout surgery but also strive to improve care and outcome by implementing different measures to keep the patient safe and promote a positive outcome during a stressful time for the surgical patient. To improve outcomes, it has been identified that hypothermia is a common occurrence in the surgical patient, especially in the GYN surgical population at this level one trauma center. Hypothermia is defined as the body’s core temperature less than 36 degrees Celsius. With hypothermia …

The Creative Arts Personal Growth Group With Centering Prayer For Sexual Trauma Survivors: A Transformative-Coping Focused Interpretative Phenomenological Analysis Study, Rachel M. Parrish-Martin

Doctoral Dissertations and Projects

Copious research demonstrates an epidemic of sexual trauma (ST) among females resulting in excruciating shame and fear of exposing oneself to others. Shame is an under-researched emerging mental health concern. This study addressed a gap in the literature regarding interventions for shame symptoms experienced by female survivors of ST. Specifically, it was an intervention study that applied a spiritually integrated form of the Creative Arts Personal Growth Group (CAPG) with Centering Prayer (CAPG-CP). Utilizing the Theory of Transformative Coping and Interpretative Phenomenological Analysis, this study explored the lived experience of nine female Christian ST survivors who participated in the CAPG-CP. …