Digital Commons Network^™

Limited Accumulation And Persistence Of An Influenza A Virus In Tadpole Snails (Physa Spp.), Paul T. Oesterle, J. Jeffrey Root, Darcy S.O. Mora, Heather Schneider, Alan B. Franklin, Kathryn P. Huyvaert

United States Department of Agriculture Wildlife Services: Staff Publications

Waterfowl infected with avian influenza A viruses (IAVs) shed infectious virus into aquatic environments, providing a mechanism for transmission among waterfowl, while also exposing the entire aquatic ecosystem to the virus. Aquatic invertebrates such as freshwater snails are likely exposed to IAVs in the water column and sediment. Freshwater snails comprise a significant portion of some waterfowl species’ diets, so this trophic interaction may serve as a novel route of IAV transmission. In these experiments, tadpole snails (Physa spp.) were exposed to a low-pathogenicity IAV (H3N8) to determine whether snails can accumulate the virus and, if so, how long virus …

A New View Of Chlorophyll Dynamics In The Southern Mid-Atlantic Bight From A Two-Year High-Resolution Spray Glider Survey, Francesco Lane

OES Theses and Dissertations

The southern Mid-Atlantic Bight (MAB) near Cape Hatteras, NC, USA, is likely a hotspot for the episodic export of carbon-rich shelf waters to the open ocean. Over a 2 year period, from March 2017 to May 2019, Spray gliders repeatedly occupied transects, along the slope and across the shelf, generating high-resolution chlorophyll fluorescence (fChl) data in the southern MAB. This study implements an fChl calibration method utilizing remotely sensing ocean color as a standard. We validate the method’s utility by demonstrating a reduction in post-calibration cross-mission fChl variability and demonstrating close correspondence between the calibrated fChl data and an in …

A Mindful Search For Basic Writing Identities: Constellating Contemplative Classroom Pedagogies, Classroom Community Circles, And Critical Discourse Analysis In A Cultural Rhetorics Ecology, Elizabeth Edwards Weems

English Theses & Dissertations

Many Basic Writing students have stories which are informed by traumas, othering, and marginalization. Finding pedagogies which address students’ stories, in addition to meeting academic goals, is crucial. Sometimes, students question how improving their reading and writing skills can help them navigate other areas of their education and experiences, especially their lives outside of academia. I have worked to create a kairotic pedagogy which invites students to read and write ‘the word and the world’ (Freire, 1970; 1988). Using Contemplative Classroom Pedagogies, I attempted to follow Freire’s (1992) call to make “teaching a creative act, a critical act, and not …

Organic Carbon Decay Mediated By Mesopelagic Microbial Communities, Noah Jonathan Craft

OES Theses and Dissertations

Substantial remineralization of organic carbon occurs in the mesopelagic zone (i.e., the biological pump), the efficiency of which is responsible for the oceans’ capacity to store carbon originally derived from the atmosphere. To better understand how a substrate’s composition influences its degradation by mesopelagic microbial communities, we added treatments made of live algal cells, dead particulate organic carbon and dissolved organic carbon from ¹⁴C-labeled algal cultures to mesopelagic water collected in-situ. Each incubation took place in the laboratory over a period of months, during which PO¹⁴C, DO¹⁴C, ATP, prokaryote abundances and biochemical fractions were measured. …

Cheiloscopy Examination And Classification Of Lip Prints With And Without Parafunctional Oral Habits: A Cross-Sectional Observation Study, Emily Smith Regan

Dental Hygiene Theses & Dissertations

Problem: Lip prints are unique and have potential for use as a human identifier. The purpose of this study was to observe possible cheiloscopy differences of individuals with and without parafunctional oral habits. Additionally, inter-rater reliability (IRR) of lip print examiners was observed. Methods: This IRB approved blinded cross-sectional observational study collected lip prints from sixty-six individuals using lipstick and adhesive tape to transfer lip prints to white bond paper for viewing purposes. Each set of included lip prints was divided into quadrants and dichotomized as those with or without an oral parafunctional habit. Each quadrant sample was manually analyzed …

Pulsed Electric Field Ablation: Mechanisms Of Differential Cell Sensitivity And Methods To Mitigate Neuromuscular Excitation, Emily Gudvangen

Biomedical Engineering Theses & Dissertations

Ablation therapies aim to destroy tumors while maintaining minimal damage to surrounding healthy tissue. Pulsed electric field (PEF) ablation targets the cell membrane during the destruction of tissues, which consequently spares extracellular structures such as ducts and blood vessels, leaving the framework for tissue regeneration. However, the utility of PEF is limited by neuromuscular excitation, causing pain and involuntary muscle contraction. To identify PEF protocols that efficiently ablate tissue while minimizing neuromuscular stimulation, various PEF protocols differing in pulse duration, shape, and repetition rate were tested. The ratio of cell death and nerve excitation thresholds, which determines the range of …

Dental Hygiene Students Reported Physiological Symptoms Associated With Wearing An N95 Respirator Mask, Peyton Shea Butler

Dental Hygiene Theses & Dissertations

Purpose: Physiological symptoms and comfort levels while wearing an N95 respiratory mask has not been examined with dental hygienists. The purpose of this study was to investigate dental hygiene students reported physiological symptoms and comfort perception while wearing an N95 respirator mask during patient care appointments. Methods: After IRB approval (IRB #1987754-2), a 16-item questionnaire was distributed through email to a convenience sample of 65 dental hygiene students. Questions assessed respiratory, dermatologic, cardiac, mask mouth and general physiological symptoms, as well as comfort levels. Additionally, participants were asked to respond to demographic questions and one open ended question inquiring about …

Investigation Of Nanosecond Pulsed Electric Fields (Nspef) Induced Anti-Cancer Mechanism And Enhanced B16f10 Melanoma Cancer Treatment, Kamal Asadipour

Biomedical Engineering Theses & Dissertations

The use of nanosecond pulsed electric fields (nsPEF) has emerged as a promising area of research with vast implications across various scientific disciplines. The ability to generate ultra-short, high-voltage electric pulses has paved the way for numerous applications, ranging from fundamental investigations of biological phenomena to the development of innovative medical therapies. The aim of this thesis is to highlight the importance of nsPEF in two critical areas: 1) Understanding the impact of subtle postpulse waveforms through a comprehensive analysis of two common pulse generators and 2) using this knowledge to advance melanoma treatment by enhancing the therapeutic effect of …

Third-Party Reactions To Performance Feedback, Daroon Mohammed Jalil

Psychology Theses & Dissertations

Although the provision of feedback has traditionally been treated as a dyadic event, I argue for the existence of a neglected third-party - the witness. Drawing from the dual process model of vicarious mistreatment and feedback intervention theory, I hypothesize that 1) third parties experience negative [positive] affect when witnessing an unjust [just] feedback event, 2) negative [positive] affect is stronger when feedback cues are self-referenced [task-referenced], and 3) negative [positive] affect is related to a subsequent decrease [increase] in feedback seeking intentions. Results from a 2x2 between-subjects experiment with 470 participants provide partial support for the hypotheses. Third-parties experienced …

Exploring The Lived Experiences Of Self-Identified Politically Conservative Students In Graduate Counseling Programs In Public Universities, Elizabeth A. Orrison

Counseling & Human Services Theses & Dissertations

Professionals within the fields of social science predominantly identify as liberal (Woessner & Kelly-Woessner, 2015) and many issues related to politics, such as power and oppression, are inherent and central to the social science fields (Lerner, 2020). Past research has shown the potential for political bias in academia, which has been linked to the lack of representation of various political identities. Most research on political academic bias is not current, making it difficult to find relevant research on this topic published within the last ten years. Currently, there has been no identified research or scholarly production that specifically explores self-identified …

Structural Insights Into The Cl-Par-4 Protein: Ionic Requirements, Conformational Transitions, And Interaction With Cisplatin, Krishna Kumar Raut

Chemistry & Biochemistry Theses & Dissertations

Cancer continues to be the leading global cause of death, with challenges in early diagnosis, drug resistance, non-specific drug targeting, and cancer recurrence and metastasis posing formidable obstacles in cancer therapy. In this context, Prostate Apoptosis Response-4 (Par-4), a pro-apoptotic tumor suppressor protein, emerged as a promising therapeutic target due to its ability to selectively induce apoptosis in cancer cells, thereby minimizing the drug-associated adverse effects. However, a comprehensive understanding of the structural features of Par-4, specifically the caspase-cleaved fragment (cl-Par-4), is crucial for therapeutic advancements.

This dissertation investigated the effects of various ions, both monovalent and divalent, on the …

Genotyping Of The Tick-Borne Pathogen Rickettsia Parkeri, Rebecca Lynn Ferrara

Biological Sciences Theses & Dissertations

Rickettsia parkeri is a pathogen of interest to public health as the causative agent of R. parkeri rickettsiosis. Amblyomma maculatum, a vector of R. parkeri, is currently undergoing a range expansion out of its historic range along the Gulf Coast of the United States, including northwards into Southeastern Virginia. Within this expansion front, the prevalence of R. parkeri within A. maculatum differs from the historic range; the historic range has a prevalence of 7.9- 38.6% while the expansion front in SE VA has a prevalence of 41.8-55.7%. While the reason for this disparity has not been established, one …

The Effects Of Self-Regulated Learning On Community College Students Metacognition, Motivation And Achievement In Geoscience Courses, Melani A. Loney

Educational Leadership & Workforce Development Theses & Dissertations

This study investigated the impact of training in self-regulated learning on community college, geoscience students’ achievement, metacognition, time management, and science motivation scales. The study also investigated the impact of SRL training on these outcomes as a function of gender and ethnicity. During the Fall of 2022, 70 community college geoscience students from 9 different classes participated in the study. The classes were bifurcated with one half of the students in each class randomly assigned to the SRL treatment and the other half to the control condition. Each week, for 10 weeks during the semester, students in the treatment group …

Do Microplastics, Vibrio Bacteria, And Warming Water Temperatures Cause Disease In The Northern Star Coral, Astrangia Poculata?, Heather Leigh Sheffey

OES Theses and Dissertations

As marine plastic pollution increases, it becomes imperative to study the effects of microplastics on marine fauna. Marine plastic debris is a vector for disease to marine invertebrates (Lamb et al., 2018; Rotjan et al., 2019). In this context, studies have shown Astrangia poculata will consume microplastics as they do their normal food (Allen et al, 2017; Rotjan et al., 2019). Further, with temperatures increasing worldwide, there is a concern the virulence of disease-causing bacteria will increase (Vezzulli et al., 2015). In a series of manipulative laboratory experiments, this study quantified changes in respiration rates and visual health of Astrangia …

Modified Vaccine Vectors To Understand Adjuvant Functions Of Listeria During Chronic Schistosomiasis, Stephanie K. Norwood

Biological Sciences Theses & Dissertations

Vaccination is one of the most effective strategies employed to prevent infectious diseases. Successful vaccination is dependent upon the induction of a specific, robust, and prolonged immune response. One of the major challenges faced by vaccine development is vaccine failure due to host-related factors that can modulate the immune system, which leads to non-responsiveness to vaccinations. The generation of new vaccine strategies is imperative to combat these effects. Live bacterial vectors are one approach used as they can elicit humoral immunity, cellular immunity, or both. Listeria monocytogenes is a Gram positive, intracellular pathogen that is an effective bacterial vaccine vector …

Fabrication Of Smooth Sac305 Thin Films Via Magnetron Sputtering And Evaluations Of Microstructure, Creep, And Electrical Resistivity, Manish Ojha

Mechanical & Aerospace Engineering Theses & Dissertations

SAC305 (96.5%Sn-3%Ag-0.5%Cu) is the leading alternative to the traditional Sn-Pb solder eutectic alloy owing to its low melting temperature, better compatibility with other components, and excellent mechanical/structural properties. In the realm of modern electronics, where devices are increasingly miniaturized, the design and characterization of thin solder joints become paramount. The orientation and size of the grains within the solder can influence its ability to withstand mechanical stresses. However, research on SAC thin films remains sparse, and these films present unique challenges and characteristics compared to their bulk counterparts, influenced by factors like interfaces, stresses, thickness, microstructure, and the nature of …

Machine Learning Approach To Activity Categorization In Young Adults Using Biomechanical Metrics, Nathan Q. C. Holland

Mechanical & Aerospace Engineering Theses & Dissertations

Inactive adults often have decreased musculoskeletal health and increased risk factors for chronic diseases. However, there is limited data linking biomechanical measurements of generally healthy young adults to their physical activity levels assessed through questionnaires. Commonly used data collection methods in biomechanics for assessing musculoskeletal health include but are not limited to muscle quality (measured as echo intensity when using ultrasound), isokinetic (i.e., dynamic) muscle strength, muscle activations, and functional movement assessments using motion capture systems. These assessments can be time consuming for both data collection and processing. Therefore, understanding if all biomechanical assessments are necessary to classify the activity …

Complex Dynamics Of Contention: Towards A Generative Model Of Social Dissent, Travis Holmes

Graduate Program in International Studies Theses & Dissertations

In 2003, McAdam et al. released their seminal work Dynamics of Contention as a challenge to scholars within the field of contentious politics. In it, they claimed that the field required a refocusing from static and topical arguments splintered into various disciplines, to a more unified research agenda in search of causal mechanisms. In the spirit of their work, this study seeks to revisit this original critique considering more recent scholarship in the fields of Complexity and Systems Sciences along with certain technological advances in the field of computational social science, namely, the Agent-Based Model (ABM). In addition, both computational …

Spontaneous Collapse As A Prognostic Marker For Human Blastocysts: A Systematic Review And Meta-Analysis, Kate Bickendorf, Fang Qi, Kelli Peirce, Jay Natalwala, Vincent Chapple, Yanhe Liu

Research outputs 2022 to 2026

STUDY QUESTION: Is spontaneous collapse (SC) by human blastocysts a prognostic factor in IVF treatment? SUMMARY ANSWER: SC in human blastocyst is associated with reduced euploid embryo and pregnancy rates. WHAT IS KNOWN ALREADY: SC of the human blastocyst is a phenomenon that was revealed relatively recently following the clinical application of time-lapse monitoring in IVF laboratories. The ploidy and clinical prognosis of affected blastocysts are still poorly understood, with inconsistent reports. Systematic reviews and meta-analyses on this topic are currently absent in the literature but its potential as a marker of embryo viability holds great clinical value. In this …

Polygenic Risk Score Predicts Risk Of Primary Sclerosing Cholangitis In Inflammatory Bowel Disease, Ming-Hsi Wang, Jessica J Friton, Laura E Raffals, Jonathan A Leighton, Shabana F Pasha, Michael F Picco, Kelly Monroe, Billy D Nix, Rodney D Newberry, William A Faubion

2020-Current year OA Pubs

BACKGROUND: Forty distinct primary sclerosing cholangitis (PSC) genomic loci have been identified through multiancestry meta-analyses. The polygenic risk score (PRS) could serve as a promising tool to discover unique disease behaviour, like PSC, underlying inflammatory bowel disease (IBD).

AIM: To test whether PRS indicates PSC risk in patients with IBD.

MATERIALS AND METHODS: Mayo Clinic and Washington University at St Louis IBD cohorts were used to test our hypothesis. PRS was modelled through the published PSC loci and weighted with their corresponding effect size. Logistic regression was applied to predict the PSC risk.

RESULTS: In total, 63 (5.6%) among 1130 …

Emotional Intelligence In Difficult Decision-Making: A Multiple-Case Study Of Community College Academic Deans, Ekaterina Kalinina Brooks

Educational Leadership & Workforce Development Theses & Dissertations

The decisions made by leaders noticeably impact employee morale and influence the fulfillment of the organizational mission. However, making decisions can be challenging when options are complex and involve multiple risks and benefits. Navigating such decisions in an era of technology when decisions are more transparent than ever before can be particularly difficult for academic deans in community colleges. These mid-level administrators often face internal conflicts stemming from the nature of their positions, campus politics, and the challenge of working between the competing interests of faculty and senior leaders.

Over the last three decades, research has pointed out that strong …

Relationship Between Learning Communities And Campus Residency On Sense Of Belonging Among First Year Undergraduates, Lanah Kathryn Stafford

Educational Leadership & Workforce Development Theses & Dissertations

In higher education, sense of belonging has been linked to improved outcomes such as retention and academic achievement (e,g., Pittman & Richmond, 2007). Previous research has also identified participation in learning communities and campus residency as interventions which improve sense of belonging for students in this context (e.g., Solanki et al., 2019; Soria & Roberts, 2021). However, little research has been conducted to empirically study sense of belonging as a multidimensional construct, and no research has investigated interactions between on-campus residency and learning community participation on its development.

Data were collected from 1,072 first-year students on the Sense of Belonging …

Regulation Of Tumor Apoptosis Of Poriae Cutis-Derived Lanostane Triterpenes By Akt/Pi3k And Mapk Signaling Pathways In Vitro, Shuai Yue, Xi Feng, Yousheng Cai, Salam A. Ibrahim, Ying Liu, Wen Huang

Faculty Research, Scholarly, and Creative Activity

Poria cocos is traditionally used as both food and medicine. Triterpenoids in Poria cocos have a wide range of pharmacological activities, such as diuretic, sedative and tonic properties. In this study, the anti-tumor activities of poricoic acid A (PAA) and poricoic acid B (PAB), purified by high-speed counter-current chromatography, as well as their mechanisms and signaling pathways, were investigated using a HepG2 cell model. After treatment with PAA and PAB on HepG2 cells, the apoptosis was obviously increased (p < 0.05), and the cell cycle arrested in the G2/M phase. Studies showed that PAA and PAB can also inhibit the occurrence and development of tumor cells by stimulating the generation of ROS in tumor cells and inhibiting tumor migration and invasion. Combined Polymerase Chain Reaction and computer simulation of molecular docking were employed to explore the mechanism of tumor proliferation inhibition by PAA and PAB. By interfering with phosphatidylinositol-3-kinase/protein kinase B, Mitogen-activated protein kinases and p53 signaling pathways; and further affecting the expression of downstream caspases; matrix metalloproteinase family, cyclin-dependent kinase -cyclin, Intercellular adhesion molecules-1, Vascular Cell Adhesion Molecule-1 and Cyclooxygenase -2, may be responsible for their anti-tumor activity. Overall, the results suggested that PAA and PAB induced apoptosis, halted the cell cycle, and inhibited tumor migration and invasion through multi-pathway interactions, which may serve as a potential therapeutic agent against cancer.

Work–Family Conflict And Its Sustainability Implications Among Married Immigrants Working In The Usa, Neena Gopalan, Nicholas J. Beutell, Joseph G. Grzywacz, Wendy Middlemiss, Srikant Manchiraju, Sapna Srivastava

Faculty Research, Scholarly, and Creative Activity

There is a paucity of research on the work and family dynamics of immigrants who arrive in the U.S. on visas. Work–family conflict among immigrants is a sustainability issue because it affects social cohesion, economic vitality, and the overall wellbeing of communities. This study examined work–family conflicts and work–life support among married immigrants (n = 182) born abroad but currently holding permanent resident status (also known as ‘green card holders’) in the U.S. Specifically, we examined how work variables (job intensity and work–life support) may either lead to or reduce job burnout, how burnout may be related to work–family conflict, …

Genomic Approaches To Mitigating Genetic Diversity Loss In Declining Populations, Christen M. Bossu, Marina Rodriguez, Christine Rayne, Debra A. Chromczak, Philip G. Higgins, Lynne A. Trulio, Kristen C. Ruegg

Faculty Research, Scholarly, and Creative Activity

The accelerating pace of global biodiversity loss is exacerbated by habitat fragmentation and subsequent inbreeding in small populations. To address this problem, conservation practitioners often turn to assisted breeding programmes with the aim of enhancing genetic diversity in declining populations. Although genomic information is infrequently included in these efforts, it has the potential to significantly enhance the success of such programmes. In this study, we showcase the value of genomic approaches for increasing genetic diversity in assisted breeding efforts, specifically focusing on a highly inbred population of Western burrowing owls. To maximize genetic diversity in the resulting offspring, we begin …

A Marine Protected Area Network Does Not Confer Community Structure Resilience To A Marine Heatwave Across Coastal Ecosystems, Joshua G. Smith, Christopher M. Free, Cori Lopazanski, Julien Brun, Clarissa R. Anderson, Mark H. Carr, Joachim Claudet, Jenifer E. Dugan, Jacob G. Eurich, Tessa B. Francis, Scott L. Hamilton, David Mouillot, Peter T. Raimondi, Richard M. Starr, Shelby L. Ziegler, Kerry J. Nickols, Jennifer E. Caselle

Faculty Research, Scholarly, and Creative Activity

Marine protected areas (MPAs) have gained attention as a conservation tool for enhancing ecosystem resilience to climate change. However, empirical evidence explicitly linking MPAs to enhanced ecological resilience is limited and mixed. To better understand whether MPAs can buffer climate impacts, we tested the resistance and recovery of marine communities to the 2014–2016 Northeast Pacific heatwave in the largest scientifically designed MPA network in the world off the coast of California, United States. The network consists of 124 MPAs (48 no-take state marine reserves, and 76 partial-take or special regulation conservation areas) implemented at different times, with full implementation completed …

Magnitude, Trends, And Variability Of The Global Ocean Carbon Sink From 1985 To 2018, Tim Devries, Kana Yamamoto, Rik Wanninkhof, Nicolas Gruber, Judith Hauck, Jens Daniel Müller, Laurent Bopp, Dustin Carroll, Brendan Carter, Thi Tuyet Trang Chau, Scott C. Doney, Marion Gehlen, Lucas Gloege, Luke Gregor, Stephanie Henson, Ji Hyun Kim, Yosuke Iida, Tatiana Ilyina, Peter Landschützer

Faculty Research, Scholarly, and Creative Activity

This contribution to the RECCAP2 (REgional Carbon Cycle Assessment and Processes) assessment analyzes the processes that determine the global ocean carbon sink, and its trends and variability over the period 1985–2018, using a combination of models and observation-based products. The mean sea-air CO2 flux from 1985 to 2018 is −1.6 ± 0.2 PgC yr−1 based on an ensemble of reconstructions of the history of sea surface pCO2 (pCO2 products). Models indicate that the dominant component of this flux is the net oceanic uptake of anthropogenic CO2, which is estimated at −2.1 ± 0.3 PgC yr−1 by an ensemble of ocean …

The Role Of Alcohol Extract Of Cranberry In Improving Serum Indices Of Experimental Metaproterenol-Induced Heart Damage In Rats, Kimia Salmasi, Ali Hassanpour, Bahram Amouoghli Tabrizi, Sina Moghaddam

Faculty, Staff and Students Publications

Cranberry offers numerous cardiovascular benefits. According to several studies, this fruit promotes the oxidation of low‐density lipoprotein, enhances high‐density lipoprotein, reduces platelet coagulation, and improves vascular activity. Albino male rats were divided into five groups (n = 5 per group). The control group received intraperitoneal administration of normal saline. The second group was injected with metaproterenol (MET) 3 days a week for 4 weeks. The third, fourth, and fifth groups were given cranberry extract in doses of 75, 100, and 150, respectively, along with heart‐damaging drugs. Blood samples were collected and sent to the laboratory on the fourth weekend …

Biomechanical Adaptations While Performing Bilateral Drop Landings With A Unilateral Ankle Tape Application, Eric Daniel Jenkins

Rehabilitation Sciences Theses & Dissertations

Ankle sprains are the most common injury in sport and exercise performance, which makes the utilization of ankle taping a common procedure to both prevent potential sprain, as well as protect against reinjury. However, unilateral ankle taping may have unintended consequences on the mechanics of the ankle and other joints of both legs. The aim of this dissertation was to determine the effects of ankle taping on lower body kinetics and kinematics, stiffness, and coordination during a bilateral landing task.

Twelve female participants completed a total of 90 drop landings across two visits, randomized from landing platforms of 30, 45, …

Nightmares And Self-Injury Among High-Risk Adolescents: Examining The Role Of Emotion Regulation, Kinjal K. Patel

Psychology Theses & Dissertations

Self-injurious thoughts and behaviors (SITBs) are a public health concern among youth. SITB rates rise drastically during adolescence -- a critical and sensitive developmental period characterized by dramatic changes in biological and social systems. Prior research has identified distal risk factors which tell us who is at risk for SITBs. Emerging research suggests proximal risk factors may be useful to identify when an individual may be at risk for SITBs. One promising proximal risk factor for SITBs is sleep problems. Although the relation between sleep problems and SITBs has been widely documented, little is known about the short-term, proximal links …