Digital Commons Network^™

Ultra-Low Intensity Post-Pulse Affects Cellular Responses Caused By Nanosecond Pulsed Electric Fields, Kamal Asadipour, Carol Zhou, Vincent Yi, Stephen J. Beebe, Shu Xiao

Electrical & Computer Engineering Faculty Publications

High-intensity nanosecond pulse electric fields (nsPEF) can preferentially induce various effects, most notably regulated cell death and tumor elimination. These effects have almost exclusively been shown to be associated with nsPEF waveforms defined by pulse duration, rise time, amplitude (electric field), and pulse number. Other factors, such as low-intensity post-pulse waveform, have been completely overlooked. In this study, we show that post-pulse waveforms can alter the cell responses produced by the primary pulse waveform and can even elicit unique cellular responses, despite the primary pulse waveform being nearly identical. We employed two commonly used pulse generator designs, namely the Blumlein …

Technology Adoption Of Computer-Aided Instruction In Healthcare: A Structured Review, Queenie Kate Cabanilla, Frevy Teofilo-Orencia, Rentor Cafino, Armando T. Isla Jr., Jehan Grace Maglaya, Xavier-Lewis Palmer, Lucas Potter, Dave E. Marcial, Lemuel Clark Velasco

Electrical & Computer Engineering Faculty Publications

Computer-Aided Instruction (CAI) is one of the interactive teaching methods that electronically presents instructional resources and enhances learner performance. In health settings, using CAI is one of the important ways to improve learners' knowledge and usefulness in their healthcare specialization yet there is still a lack of research that offers a comprehensive synthesis of investigating into the adoption of CAI in healthcare. This research aims to provide a comprehensive review of related literatures on the enablers and barriers for technology adoption of CAI in healthcare. 31 journals were analyzed and revealed that several studies were utilizing the Unified Theory of …

An Explainable Artificial Intelligence Framework For The Predictive Analysis Of Hypo And Hyper Thyroidism Using Machine Learning Algorithms, Md. Bipul Hossain, Anika Shama, Apurba Adhikary, Avi Deb Raha, K. M. Aslam Uddin, Mohammad Amzad Hossain, Imtia Islam, Saydul Akbar Murad, Md. Shirajum Munir, Anupam Kumur Bairagi

Electrical & Computer Engineering Faculty Publications

The thyroid gland is the crucial organ in the human body, secreting two hormones that help to regulate the human body's metabolism. Thyroid disease is a severe medical complaint that could be developed by high Thyroid Stimulating Hormone (TSH) levels or an infection in the thyroid tissues. Hypothyroidism and hyperthyroidism are two critical conditions caused by insufficient thyroid hormone production and excessive thyroid hormone production, respectively. Machine learning models can be used to precisely process the data generated from different medical sectors and to build a model to predict several diseases. In this paper, we use different machine-learning algorithms to …

Social-Engineering, Bio-Economies, And Nation-State Ontological Security: A Commentary, Brandon Griffin, Keitavius Alexander, Xavier-Lewis Palmer, Lucas Potter

Electrical & Computer Engineering Faculty Publications

Biocybersecurity is an evolving discipline that aims to identify the gaps and risks associated with the convergence of Biology (the science of life and living organisms) and cybersecurity (the science, study, and theory of cyberspace and cybernetics) to protect the bioeconomy. The biological industries’ increased reliance on digitization, automation, and computing power has resulted in benefits for the scientific community, it has simultaneously multiplied the risk factors associated with industrial espionage and the protection of data both commercial and proprietary. The sensitive and potentially destructive power of this data and its access inherently poses a risk to the national and …

Implications Of Cyberbiosecurity In Advanced Agriculture, Simone Stephen, Keitavius Alexander, Lucas Potter, Xavier-Lewis Palmer

Electrical & Computer Engineering Faculty Publications

The world is currently undergoing a rapid digital transformation sometimes referred to as the fourth industrial revolution. During this transformation, it is increasingly clear that many scientific fields are not prepared for this change. One specific area is agriculture. As the sector which creates global food supply, this critical infrastructure requires detailed assessment and research via newly developed technologies (Millett et al, 2019; Peccoud et al, 2018) . Despite its fundamental significance to modern civilization, many aspects of industrial agriculture have not yet adapted to the digital world. This is evident in the many vulnerabilities currently present within agricultural systems, …

A Reflection Of Typology And Verification Flaws In Consideration Of Biocybersecurity/Cyberbiosecurity: Just Another Gap In The Wall, Luke Potter, Kim Mossberg, Xavier Palmer

Electrical & Computer Engineering Faculty Publications

Verification is central to any process in a functional and enduring cyber-secure organization. This verification is how the validity or accuracy of a state of being is assessed (Schlick, 1936; Balci, 1998). Conversely, breakdown in verification procedures is core to the interruption of normal operations for an organization. A key problem for organizations that utilize biology as an interlock within their systems is that personnel lack sufficient ability to verify all practically relevant biological information for procedures such as a nurse logging a blood draw, or a molecular biology technician preparing agar to culture microbes for study. This has several …

Carbohydrates And Endurance Exercise: A Narrative Review Of A Food First Approach, Alireza Naderi, Nathan Gobbi, Ajmol Ali, Erfan Berjisian, Amin Hamidvand, Scott C. Forbes, Majid S. Koozehchian, Raci Karayigit, Bryan Saunders

Research, Publications & Creative Work

Carbohydrate (CHO) supplements such as bars, gels, drinks and powders have become ubiquitous as effective evidence-based CHO sources that improve endurance exercise performance. However, athletes are increasingly turning to more cost-effective ‘food-first’ approaches for CHO ingestion to improve exercise performance. Mixed CHO foods including cooked lentils, oats, honey, raisins, rice, and potatoes are all effective pre-exercise CHO food sources. Caution is advised when selecting some of these foods as a primary CHO source, as some athletes may be prone to gastrointestinal discomfort—especially regarding those foods where the quantities required for recommended CHO intake may be voluminous (e.g., potatoes). Palatability may …

Physical Activity And The Link Among Stress, Burnout, And Well-Being In Athletic Trainers, Christine E. Pacewicz, Taylor W. Rowley, Jennifer L. Savage

Research, Publications & Creative Work

Stress is a leading cause of burnout in working professionals, including athletic trainers (ATs). One consequence of burnout for ATs is lower perceptions of well-being, which have implications for mental and physical health. Physical activity is known to help reduce stress. Thus, activity may help reduce stress and burnout in ATs while enhancing well-being.

Distribution Patterns And Factors Influencing Relative Abundance Of The Alligator Snapping Turtle (Macrochelys Temminckii) In Mississippi, Luke Pearson, Lucas Haralson, Gabrielle Berry, Grover J. Brown, Carl Qualls

Research, Publications & Creative Work

Macrochelys temminckii (Alligator Snapping Turtle) was recently proposed as a threatened species under the US Endangered Species Act due to current and projected future declining populations range wide. Despite a presumed statewide distribution in Mississippi, the Alligator Snapping Turtle had been documented in only 32 of 82 counties between 1857 and 2012, thus leaving a substantial gap in our knowledge of this species’ distribution. Therefore, we collected credible records from museums, literature, and the general public, as well as directly assessed the current distribution and relative abundance of the Alligator Snapping Turtle in all major river systems (i.e., Pascagoula, Pearl, …

Targeting The Subacute Progression Of Hippocampal Cellular Senescence Following Repetitive Mild Traumatic Brain Injury, Jacob Edward Exline

Master's Theses

Repetitive mild traumatic brain injury (rmTBI) increases an individual’s risk for developing persistent deficits in recognition memory with symptoms experienced by up to 30% of rmTBI patients. Cellular senescence has been suggested as an underlying mechanism of persistent rmTBI symptoms; however, the subacute progression of cellular senescence following rmTBI has not been evaluated. Furthermore, the use of subacute testosterone administration to treat persistent recognition memory deficits and the cellular and molecular underpinnings has yet to be well characterized. Our lab utilizes a 5-hit closed-head rmTBI rat model to induce deficits in recognition memory and determine the effects of subacute testosterone …

Investigating Putative Toxin-Immunity Pairs From A New Class Of Rhs Proteins In Pseudomonas Aeruginosa, Syed Faieq Imam

Master's Theses

Pseudomonas aeruginosa is a gram-negative, opportunistic pathogen that is ubiquitous in nature, especially in human/animal impacted environments. One of the reasons for this is the arsenal of protein systems and toxins that P. aeruginosa utilizes to mold its environment to suit its needs. Recently, we discovered a novel locus in the P. aeruginosa genome, which resembles other loci that are known to mediate interbacterial competition. Our bioinformatic analysis found this locus to be enriched in a rearrangement hotspot (rhs) motif which led us to ad-interim term the three ORFs of the loci as rhsB, rhsC, and rhsI. Furthermore, we have …

Abortion, Medieval Christianity, And The Christian Far-Right: The Harms Of Rhetoric And Misconstruing The Past, Madison Givens

Master's Theses

This research explores the intersection of abortion, Medieval Christianity, and the Christian far-right, with a focus on the harms of rhetoric and the misconstruing of history. The study argues that a selective reading of history is being used by the Christian far-right to justify violence against reproductive rights activists, including police brutality and mass shootings, as well as to promote forced sterilization and other forms of reproductive control. This thesis examines the harms of rhetoric and misconstruing the past in the abortion and medieval Christianity debate. By analyzing the role of language, symbols, and history in shaping the abortion debate …

Effect Of Selenof Loss In Combination With A Western Diet On Tumorigenesis, Brenna Joy Flowers

Master's Theses

SELENOF is an understudied selenium-containing protein that has previously been postulated to behave as a tumor suppressor in the breast. Examination of patient databases showed that SELENOF levels were lowest in tumors from patients with aggressive late-stage breast cancers. Whether loss of SELENOF drives breast tumorigenesis remains to be determined. To address this question, we used juvenile female wild type or systemic Selenof knockout mice and exposed them to 7,12-Dimethylbenz[a]anthracene (DMBA), a carcinogen that replicates the multistep process of breast tumorigenesis. Previous reports have shown that loss of Selenof led to glucose and metabolic dysregulation in mice suggesting a link …

How To Build A Cortex: Coordinated Assembly Of Cortical Septins And Actomyosin In The Leader Bleb, Asia Marie Paguntalan

Master's Theses

In confined spaces, migrating cells can undergo mesenchymal-to-amoeboid transitions by altering their cortical dynamics and adhesion with the environment. Septins frequently associate with cortical actin and non-muscle myosin (NMII), but the functional nature of these interactions remains unclear. Upon non-adhesive confinement and NMII enrichment, fibroblasts can switch to a fast, leader bleb-based mode of motility, characterized by the absence of adhesions and stress fibers and formation of a single, elongated leader bleb. During this transition, cortical actin remodeling and polarized NMII contractility drive leader bleb stabilization by generating long-range cortical flows, in coordination with changes in septin localization and assembly …

Moral Formation In A Culture Of Relativism: Correlates Of Universalism And Relativism In The Moral Outlooks Of Emerging Adults, Steven Crawford Hayward

Journal of Catholic Education

This study explores some of the demographic, personal, and experiential factors of emerging adults that correlate to a spectrum of moral outlooks ranging from moral universalism to moral relativism. Data was gathered from 466 volunteer undergraduate students. Respondents’ demographic, experiential, and personal factors served as independent variables. The relativism index score form Forsyth’s Ethics Position Questionnaire (1980) served as the dependent variable. Results suggest several conclusions: First, adults in the life of youth are a significant factor in moral outlook, exerting influence in both universalistic and relativistic directions. Second, religious practice and involvement contribute to a universal moral outlook. Third, …

Exploring The Status Of Transgender Students In Catholic High Schools, Dirk De Jong

Journal of Catholic Education

This paper reports on a recent survey of principals of Catholic high schools across the country regarding the existence of formal gender identity policies or informal practices with respect to the behavior and treatment of transgender students in their schools. The survey’s findings are discussed in the context of recent developments with respect to the science, clinical interventions, and legal accommodations surrounding gender variance. The paper also describes the political developments with respect to this issue and some of the pushback in communities of faith. It concludes by suggesting the need for receptivity to scientific findings as part of a …

Identification Of New Substrates From Pseudomonas Aeruginosa Exos, Adam V. Thota

Master's Theses

Pseudomonas aeruginosa is a gram-negative environmental bacterium that uses its intrinsic and acquired antibiotic resistance to infect patients with compromised epithelium, such as cystic fibrosis and burn wounds, and corneal keratitis. In order to cause disease, most strains of P. aeruginosa use the type three secretion system (T3SS) to disrupt cellular signaling and integrity. However, P. aeruginosa may also secrete exotoxins after invading host cells, which prolongs host cell death and maintains an intracellular niche. Of the T3 secreted effector toxins, ExoS, specifically the ADP-ribosyltransferase activity of ExoS, was shown to delay the death of invaded corneal cells, but it …

Characterization Of The Full-Length Bag3 Protein And Stress Induced Formation Of Bag3-Z, Ahmed Gamal Abdalla Zied

Master's Theses

Bcl2-associated athanogene-3 (BAG3) is a pro-autophagy co-chaperone that we havepreviously shown localizes to the cardiac sarcomere and is critical for proteostasis and maintenance of normal sarcomeric function. Indeed, BAG3 loss in heart failure (HF) results in accumulation of ubiquitinated sarcomeric proteins, and depressed maximum force generating capacity (Fmax). However, how BAG3 is regulated in the cell is not well understood, with uncertainty about its structure and proteoforms. During our analysis of human heart tissue, BAG3 appears as a “doublet”, with one band at 74 kDa (BAG3-Z) and a second at a higher 85 kDa (BAG3-FL). Previous studies hypothesized the full-length …

Incorporating Sex Chromosomes In Transcriptome Prediction Models And Improving Cross-Population Prediction Performance, Daniel S. Araujo

Master's Theses

Transcriptome prediction models built with data from European-descent individuals are less accurate when applied to different populations because of differences in linkage disequilibrium patterns and allele frequencies. We hypothesized multivariate adaptive shrinkage may improve cross-population transcriptome prediction, as it leverages effect size estimates across different conditions - in this case, different populations. To test this hypothesis, we made transcriptome prediction models for use in transcriptome-wide association studies (TWAS) using different methods (Elastic Net, Matrix eQTL and Multivariate Adaptive Shrinkage in R (MASHR)) and tested their out-of-sample transcriptome prediction accuracy in population-matched and cross-population scenarios. Additionally, to evaluate model applicability in …

Comparison Of Taxonomy Assignment And Strain Detection In Urobiome Communities Using 16s Rrna Sequencing And Shotgun Metagenomic Sequencing, Delaney Sauer

Master's Theses

Advancements in sequencing technologies have enabled scientists to gain insight into the microbes that inhabit the human body, including the urinary tract. Cataloging the bacteria that inhabit the urinary tract has primarily relied on amplification and sequencing of specific variable regions of the 16S rRNA gene enabling genus-level taxonomic identification. Recently, shotgun metagenomic sequencing has been employed such that bacterial taxonomy as well as the functionality that they encode can be inferred. In this study, we compare taxonomies assigned by 16S sequencing and shotgun metagenomic sequencing of the urinary microbiota (urobiome) of females with and without a clinical diagnosis of …

Variation In Carapace Damage Within And Among Loggerhead Musk Turtle (Sternotherus Minor) Populations In Florida Spring-Fed Ecosystems, Joseph J. Pignatelli Iii, Katrina Campbell, Brian P. Butterfield, Gerald R. Johnston, Joseph C. Mitchell, Grover J. Brown, J. Brian Hauge, Andrew D. Walde, Eric C. Munscher

Research, Publications & Creative Work

Damage to a turtle’s shell can provide evidence of past events such as vehicle collisions, disease, predator encounters, or even a behavioural interaction between members of the same species. Documenting shell damage as part of long-term mark and recapture studies enables researchers to determine population trends, intraspecific interactions and identify potential issues within turtle populations. This paper analyses shell damage in populations of the Loggerhead Musk Turtle (Sternotherus minor) (Agassiz, 1857). We examined carapace shell damage frequency and severity in 2701 individual S. minor (1468 males and 1233 females) captured in spring-fed habitats in one state preserve and five state …

Functional Comparison Of Full-Length Palladin To Isolated Actin Binding Domain, Sharifah Albraiki, Oluwatosin Ajiboye, Rachel Sargent, Moriah R. Beck

Research, Publications & Creative Work

Palladin is an actin binding protein that is specifically upregulated in metastatic cancer cells but also colocalizes with actin stress fibers in normal cells and is critical for embryonic development as well as wound healing. Of nine isoforms present in humans, only the 90 kDa isoform of palladin, comprising three immunoglobulin (Ig) domains and one proline-rich region, is ubiquitously expressed. Previous work has established that the Ig3 domain of palladin is the minimal binding site for F-actin. In this work, we compare functions of the 90 kDa isoform of palladin to the isolated actin binding domain. To understand the mechanism …

Gross Ecosystem Product Accounting Of A Globally Important Agricultural Heritage System: The Longxian Rice–Fish Symbiotic System, Jianfang Liu, Qian Zhang, Qianyi Wang, Yaoping Lv, Yingqi Tang

Research, Publications & Creative Work

The Rice–Fish Symbiotic System in the Longxian village is the first Globally Important Agricultural Heritage System (GIAHS) in Asia. This paper examined the value transformation mechanism of the Longxian Rice–Fish Symbiotic System from the micro perspective of material cycle and energy flow and quantified its economic value using the gross ecosystem product (GEP). Using the InVEST model and ArcGIS tools, the GEP was estimated to be approximately CNY 116.51 million (approx. CNY 252,624 per hectare per year). The ecosystem regulating service value occupied more than 80% of the GEP. Within the regulating ecosystem services, the climate regulation value was the …

Dna Barcoding Indicates Multiple Invasions Of The Freshwater Snail Melanoides Tuberculata Sensu Lato In Florida, Lori Tolley-Jordan, Michael A. Chadwick, Jimmy K. Triplett

Research, Publications & Creative Work

Melanoides tuberculata sensu lato (Thiaridae) are polymorphic female-clonal snails of Asian and African origins that have invaded freshwaters worldwide, including those in Florida. Although the snails have been documented in Florida for at least 70 years, no studies have investigated whether the observed distribution is due to a single introduction or multiple independent invasions. Here, cytochrome oxidase I was used to measure genetic diversity within and among sites in Florida and compare genetic diversity between Florida and other regions of the world. We also examined the relationship between shell morphology and haplotype diversity to determine if shell morphs can serve …

Investigating The Viral Nature Of Neuronal Capsid Genes, Michael Manuel Long

Master's Theses

Memory formation is an essential part of everyday life. Neuronal communication is thought to be a key driver behind memory formation. There are two heavily studied methods of neuronal communication: chemical (neurotransmitter-mediated) and electrical (physically attached). When issues arise environmentally or genetically, people can experience one of many devastating memory disorders i.e. Alzheimer’s disease and other Dementias. Due to their prevalence, the treatments for disorders that hinder memory formation are a high priority for pharmaceutical companies. Currently, many of the treatments available focus on neurotransmitters or neuronal activity, and scientists are far from a cure. However, there may be an …

Characterizing The Dynamic Localization Of Cmi In Early Drosophila Development, Asra Habibullah

Master's Theses

The COMPASS-like family of lysine methyltransferases, MLR/MLX complexes, are epigenetic regulators that are essential for normal development through the methylation of the fourth lysine residue on histone 3 (H3K4), a universal epigenetic mark associated with active transcription. This family of complexes is highly conserved from yeast to mammals and the genes encoding the human MLR complexes have been associated with various developmental diseases and cancers (Dingwall and Fagan, 2019). In D. melanogaster, the enzymatic methyltransferase core of this complex is composed of two proteins: Cara Mitad (Cmi, also known as Lpt) and Trithorax-related (Trr). Although these proteins have been shown …

Making The Case For The Great Dismal Swamp National Heritage Area: A Scoping Review, Madelyn Newton, Chandler J. Berry, Bethany Arrington, Nick Wilson, Colin Mccormack, Michael Wilcox, Alexis Barmoh, Chris A. B. Zajchowski

Human Movement Studies & Special Education Faculty Publications

National Heritage Areas (NHAs) are nationally distinct landscapes that represent unique cultural, historical, and/or natural attributes significant to the legacy of the United States of America (U.S.). The Great Dismal Swamp, located in southeastern Virginia and northeastern North Carolina, is a prime candidate for NHA designation with diverse qualifications, among which was its antebellum role as a refuge for formerly enslaved people. The goal of our research, conducted in 2022 during the period of the U.S. Congressional debate on designation, was to investigate and expound upon the rationale for NHA designation of the Swamp. To do so, we used a …

The Effect Of Training Program On Autism Spectrum Quotient Scores, Hossein Nazary Sharif, Sarvin Salar, Justin Anthony Haegele

Human Movement Studies & Special Education Faculty Publications

Background: Autism Spectrum Quotient (AQ) functions as an evaluative instrument designed to assess traits associated with autism. Variances exist within the brain of individuals on the autism spectrum. Moreover, certain exercises have the potential to impact specific regions of the brain.

Aim: This study aims to investigate how the program training of this study impact Autism—Spectrum Quotient.

Materials and Methods: Forty-two children aged 7-10 years with ASD were recruited to participate in this study. These children were randomly divided into two groups: the control group (N=10, 7.80±1.35 years) and the experimental group (N=32, age: 8.16±1.16 years). The experimental group underwent …

Understanding Optimisation Processes With Biologically-Inspired Visualisations, Mathew Walter

School of Engineering, Computing and Mathematics Theses

Evolutionary algorithms (EAs) constitute a branch of artificial intelligence utilised to evolve solutions to solve optimisation problems abound in industry and research. EAs often generate many solutions and visualisation has been a primary strategy to display EA solutions, given that visualisation is a multi-domain well-evaluated medium to comprehend extensive data. The endeavour of visualising solutions is inherent with challenges resulting from high dimensional phenomenons and the large number of solutions to display. Recently, scholars have produced methods to mitigate some of these known issues when illustrating solutions. However, one key consideration is that displaying the final subset of solutions exclusively …

Is This Antiracist?: An Autoethnographic Evaluation Of Professional Development, Allison Nava-Holstein

LMU Theses and Dissertations

Communities of color have been fighting for equal rights in society throughout history, resulting in the development of different social movements. In 2013, the Black Lives Matter movement was founded and protests erupted across America as Black lives were lost at the hands of law enforcement. In the summer of 2020, the protests continued, serving as a catalyst for schools to begin interrogating their practices and curricula, moving towards efforts to be pro-Black and antiracist.

Grounded in Critical Social Theory and Critical Race Theory, this autoethnographic study explores the experiences of me, a Latinx teacher in diversity, equity and inclusion …