Proteome Changes Resulting From Malting In Hordein-Reduced Barley Lines, Mahya Bahmani, Angéla Juhász, Utpal Bos, Mitchell G. Nye-Wood, Malcolm Blundell, Crispin A. Howitt, Michelle L. Colgrave

Research outputs 2022 to 2026

Hordeum vulgare L., commonly known as barley, is primarily used for animal feed and malting. The major storage proteins in barley are hordeins, known triggers of celiac disease (CD). Here, sequential window acquisition of all theoretical mass spectra (SWATH)-MS proteomics was employed to investigate the proteome profile of grain and malt samples from the malting barley cultivar Sloop and single-, double-, and triple hordein-reduced lines bred in a Sloop background. Using a discovery proteomics approach, 2688 and 3034 proteins were detected from the grain and malt samples, respectively. By utilizing label-free relative quantitation through SWATH-MS, a total of 2654 proteins …

Bidirectional Causality Between Immunoglobulin G N-Glycosylation And Metabolic Traits: A Mendelian Randomization Study, Xiaoni Meng, Weijie Cao, Di Liu, Isinta M. Elijah, Weijia Xing, Haifeng Hou, Xizhu Xu, Manshu Song, Youxin Wang

Research outputs 2022 to 2026

Although the association between immunoglobulin G (IgG) N-glycosylation and metabolic traits has been previously identified, the causal association between them remains unclear. In this work, we used Mendelian randomization (MR) analysis to integrate genome-wide association studies (GWASs) and quantitative trait loci (QTLs) data in order to investigate the bidirectional causal association of IgG N-glycosylation with metabolic traits. In the forward MR analysis, 59 (including nine putatively causal glycan peaks (GPs) for body mass index (BMI) (GP1, GP6, etc.) and seven for fasting plasma glucose (FPG) (GP1, GP5, etc.)) and 15 (including five putatively causal GPs for BMI (GP2, GP11, etc.) …

Coastal Groundwater-Dependent Ecosystems Are Falling Through Policy Gaps, Madeleine Dyring, Melissa M. Rohde, Ray Froend, Harald Hofmann

Research outputs 2022 to 2026

Coastal groundwater-dependent ecosystems (GDEs), such as wetlands, estuaries and nearshore marine habitats, are biodiversity hotspots that provide valuable ecosystem services to society. However, coastal groundwater and associated ecosystems are under threat from groundwater exploitation and depletion, as well as climate change impacts from sea-level rise and extreme flood and drought events. Despite many well-intentioned policies focused on sustainable groundwater use and species protection, coastal GDEs are falling through gaps generated by siloed policies and as a result, are declining in extent and ecological function. This study summarized then examined policies related to the management of coastal groundwater and connected ecosystems …

Orthodox Vs Paradox: The Roles Of Glycomics, Genetics And Beyond In Immunity, Immune Disorders And Glycomedicine, Wei Wang

Research outputs 2022 to 2026

The traditional ‘central dogma’ describes the flow of genetic information from DNA to RNA to protein. This process highlights the critical role of genes in living organisms. Nonetheless, ongoing immunological research coming to realize that emerging disciplines like glycomics and epigenetics are challenging the traditional viewpoint and extending the boundaries of the ‘central dogma’. . . .

Genetic And Immunological Insights Into Covid-19 With Acute Myocardial Infarction: Integrated Analysis Of Mendelian Randomization, Transcriptomics, And Clinical Samples, Zequn Zheng, Yueran Zhou, Yongfei Song, Pengxiang Ying, Xuerui Tan

Research outputs 2022 to 2026

Background: Globally, most deaths result from cardiovascular diseases, particularly ischemic heart disease. COVID-19 affects the heart, worsening existing heart conditions and causing myocardial injury. The mechanistic link between COVID-19 and acute myocardial infarction (AMI) is still being investigated to elucidate the underlying molecular perspectives. Methods: Genetic risk assessment was conducted using two-sample Mendelian randomization (TSMR) to determine the causality between COVID-19 and AMI. Weighted gene co-expression network analysis (WGCNA) and machine learning were used to discover and validate shared hub genes for the two diseases using bulk RNA sequencing (RNA-seq) datasets. Additionally, gene set enrichment analysis (GSEA) and single-cell RNA-seq …

Corticosteroid-Laba Inhalers Increase Phonation Threshold Pressure (Ptp) And Flow (Ptf) In Rabbits, Christopher Dromey, Kristine Tanner, Heidi J. Robison, Maya Elena Stevens, Ray M. Merrill, Julie Barkmeier-Kraemer, Ben Christensen

Faculty Publications

Objectives: This study examined the effects of a combination corticosteroid plus long-acting beta2-adrenergic agonist inhaler (IC) on rabbit phonation. Methods: White New Zealand male rabbits were assigned randomly to experimental and control groups (n = 11 per group). The experimental group received twice-daily doses of Advair HFA™ (fluticasone propionate 45 mcg and salmeterol 21 mcg) via a veterinary facemask with 1-way valve and spacer; the control group received aerosolized saline. After 8 weeks, animals were euthanized, larynges excised, frozen, and subsequently thawed and mounted on a standard bench apparatus. Phonation was elicited during 15 successive trials, and phonation threshold pressure …

Philippine Gabaldon Heritage School Buildings In The New Normal : Safety, Sustainability And Human Development Perspectives, Inero Ancho

Journal of Urban Culture Research

As the COVID-19 pandemic has impacted education, stakeholders find means to return to the New Normal through limited face-to-face learning. The Philippine Gabaldon School Buildings (GSBs) are seen as venues where this can happen. Built from 1908 –1915, GSBs were erected during the American colonial government. Republic Act 11194 mandates these buildings’ conservation. Due to its perceived astounding structure and cultural value, a qualitative study was conducted that delved into the existing publicly available documents related to the buildings, for in-depth analysis. The investigation highlights how GSBs potentially address the minimum health standards relevant to the pandemic and limited face-to-face …

Allele-Specific Quantification Of Human Leukocyte Antigen Transcript Isoforms By Nanopore Sequencing, Andrew E O Hughes, Maureen C Montgomery, Chang Liu, Eric T Weimer

2020-Current year OA Pubs

INTRODUCTION: While tens of thousands of HLA alleles have been identified by DNA sequencing, the contribution of alternative splicing to HLA diversity is not well characterized. In this study, we sought to determine if long-read sequencing could be used to accurately quantify allele-specific HLA transcripts in primary human lymphocytes.

METHODS: cDNA libraries were prepared from peripheral blood lymphocytes from 12 donors and sequenced by nanopore long-read sequencing. HLA reads were aligned to donor-specific reference sequences based on the known type of each donor. Allele-specific exon utilization was calculated as the proportion of reads aligning to each allele containing known exons, …

An Image-Based Modeling Framework For Predicting Spatiotemporal Brain Cancer Biology Within Individual Patients, Kamila M Bond, Lee Curtin, Sara Ranjbar, Ariana E Afshari, Leland S Hu, Joshua B Rubin, Kristin R Swanson

2020-Current year OA Pubs

Imaging is central to the clinical surveillance of brain tumors yet it provides limited insight into a tumor's underlying biology. Machine learning and other mathematical modeling approaches can leverage paired magnetic resonance images and image-localized tissue samples to predict almost any characteristic of a tumor. Image-based modeling takes advantage of the spatial resolution of routine clinical scans and can be applied to measure biological differences within a tumor, changes over time, as well as the variance between patients. This approach is non-invasive and circumvents the intrinsic challenges of inter- and intratumoral heterogeneity that have historically hindered the complete assessment of …

Updates In The Chronic Kidney Disease-Mineral Bone Disorder Show The Role Of Osteocytic Proteins, A Potential Mechanism Of The Bone-Vascular Paradox, A Therapeutic Target, And A Biomarker, Matthew J Williams, Sarah C White, Zachary Joseph, Keith A Hruska

2020-Current year OA Pubs

The chronic kidney disease-mineral bone disorder (CKD-MBD) is a complex multi-component syndrome occurring during kidney disease and its progression. Here, we update progress in the components of the syndrome, and synthesize recent investigations, which suggest a potential mechanism of the bone-vascular paradox. The discovery that calcified arteries in chronic kidney disease inhibit bone remodeling lead to the identification of factors produced by the vasculature that inhibit the skeleton, thus providing a potential explanation for the bone-vascular paradox. Among the factors produced by calcifying arteries, sclerostin secretion is especially enlightening. Sclerostin is a potent inhibitor of bone remodeling and an osteocyte …

Testing Of A Novel Questionnaire Of Household Exposure To Wood Smoke, Paula M. Meek, Sarah J. Schmiege, Akshay Sood, Hans Petersen, Rodrigo Vazquez-Guillamet, Hammad Irshad, Jacob Mcdonald, Yohannes Tesfaigzi

2020-Current year OA Pubs

Household air pollution from wood smoke (WS), contributes to adverse health effects in both low- and high-income countries. However, measurement of WS exposure has been limited to expensive in-home monitoring and lengthy face-to-face interviews. This paper reports on the development and testing of a novel, self-report nine-item measure of WS exposure, called the Household Exposure to Wood Smoke (HEWS). A sample of 149 individuals using household wood stoves for heating from western states in the U.S., completed the HEWS during the winter months (November to March) of 2013 through 2016 with 30 subjects having in-home particle monitoring. Hard copy or …

The Relationship Between Adverse Childhood Experiences And Resilience Through The Eyes Of Transition, Andrew J. Dies

College of Graduate Studies: Theses & Dissertations

This study focuses on the role that Adverse Childhood Experiences play in the lives of today’s college students and how Protective Factors can be integrated and enhanced to increase a student’s level of Resilience. This descriptive, causal-comparative quantitative study looked at participants’ levels of Resilience, what Adverse Childhood Experiences they entered college with, and what role Protective Factors played in mitigating the impacts of those Adverse Childhood Experiences on the levels of Resilience. Results indicated older students scored a higher Resilience score than their younger counterparts. Goal efficacy had the strongest effect on Resilience levels, while planning and goal efficacy …

Acute Effects Of Trunk Stretching On Aortic Arterial Stiffness And Central And Peripheral Blood Pressure In Middle-Aged Older Adults, Kylee West

College of Graduate Studies: Theses & Dissertations

BACKGROUND: Physical activity may alter the trajectory of age-related arterial stiffening, which is associated with cardiovascular morbidity and mortality. Trunk stretching has reduced arterial stiffness (AS) in young males, but its effect in middle-aged to older adults is unclear. This study examined the effects of passively assisted trunk stretching (PATS) on aortic arterial stiffness and central and peripheral blood pressure in middle-aged to older adults. METHODS: Carotid-femoral pulse wave velocity (cf-PWV) and Augmentation Index normalized to a heart rate of 75 beats per minute (AIx75) were measured in 28 middle-aged to older adults (14M/14F; 72 ± 7 years; 28.5 …

Constructing (Un)Situated Women: Situated Knowledges In Arundhati Roy's The God Of Small Things (1997) And Balli K. Jaswal's Erotic Stories For Punjabi Widows (2017), Necole T. Deloach

College of Graduate Studies: Theses & Dissertations

This thesis applies Donna Haraway’s concept of “situated knowledges” to postcolonial feminist novels such as Arundhati Roy’s The God of Small Things and Balli K. Jaswal’s Erotic Stories for Punjabi Widows in order to illustrate why there needs to be a new framework for analyzing literary postcolonial women. Despite the applicability of Donna Haraway’s “situated knowledges” to postcolonial feminist literary studies, there has been little research published that analyzes not just the intersection of “situated knowledges” and postcolonial feminist literature, but also the problems that occur when Western scholars approach postcolonial texts without completely acknowledging their own worldview. I argue …

Can Gratitude Lists Mitigate Burnout In First-Year College Students?, Catherine Jones

College of Graduate Studies: Theses & Dissertations

In recent years there has been a significant uptick in people expressing feelings of being burnt out. Many studies have looked at burnout in other students, such as college athletes, but none have looked at the true freshman first-year student. This study applied this intervention to a group of first-year college students to examine the impact of making gratitude lists on feelings of burnout. When comparing a control group that did not engage in gratitude list making, there was no statistically significant difference in feelings of burnout. However, correlation analyses revealed connections between gratitude and burnout variables that may lay …

Hepatic Transcriptomic Assessment Of Sprague Dawley Rats In Response To Dietary Perfluorobutane Sulfonate (Pfbs) Ingestion, Isaac Appiah

College of Graduate Studies: Theses & Dissertations

Perfluorobutane sulfonate (PFBS) is a short-chain PFAS that is considered to be a less toxic replacement for the rather more toxic long-chain perfluorooctane sulfonate (PFOS). Its numerous industrial applications and widespread presence in the environment have raised environmental and health concerns because of growing evidence associating adverse health effects and certain liver diseases to PFBS exposure. The study goal was to investigate whether dietary ingestion of PFBS would induce liver injuries, damage, inflammations, and oxidative stress. To achieve these goals, Sprague-Dawley rats were assigned into three PFBS dietary treatment groups (0, 50, and 100 PPM) for 11 weeks. After this …

Evaluation Of The Loving Support Peer Counseling Program On Breastfeeding Duration Rates Among African American Women Enrolled In The Program (2017-2018), Nancy S. Jeffery

College of Graduate Studies: Theses & Dissertations

Mothers and babies form an inseparable biological and social unit; the health and nutrition of one group cannot be divorced from the health and nutrition of the other.”-World Health Organization. The aim of this study was to evaluate the effectiveness of the Loving Support Peer Counseling (LSPC) program, under the WIC program in the state of Georgia, on breastfeeding duration rates among African American postpartum primiparas women. This observational study analyzed state-level data from January 2017 through December 2018. A cross-sectional design was used to evaluate the effectiveness of the Loving Support Peer Counseling Program (LSPC) in six of the …

Assessing The Influence Of Gender Equity In The Us State Legislatures On Prenatal Healthcare Utilization, Prachi Joshi

College of Graduate Studies: Theses & Dissertations

Prenatal care is crucial for achieving public health and primary healthcare objectives. It is one of the goals of Healthy People 2030, which is the US federal initiative to improve the health and well-being of people nationwide. Unfortunately, despite the lofty goals, the United States has the highest maternal mortality rate among developed countries. In 2020, the maternal mortality rate in the US was 24 deaths per 100,000 live births, more than three times the rate in most high-income countries. What is concerning is that it is getting worse. Seven hundred women die of pregnancy-related complications. Among them, black and …

Fabrication And Investigation Of Microfluidic Devices That Produce Non-Linear Chemical Gradients, Elijah L. Waters

College of Graduate Studies: Theses & Dissertations

Investigation of cell chemotaxis requires controlled chemical gradients. We investigated microfluidic devices that could enhance small populations' cell assays because of their ability to generate various chemical gradients. Our five designs generate different chemical concentration landscapes that we can easily convert into tools to study cell response to growth factors. Gradient landscapes occurred by splitting and mixing two input fluid concentrations using bifurcations, trifurcations, and Y-mixing junctions in three consecutive steps. Such fluid flow manipulations resulted in nine concentration streams entering a 0.54-mm-wide gradient chamber. The first design used a 1:1 ratio Y-mixer (unbiased) when blending two concentrations, resulting in …

Implicit Beliefs About Anxiety And The Relationship With Competitive Anxiety Intensity And Direction, Luca M. Ziegler

College of Graduate Studies: Theses & Dissertations

During the Fall of 2021, 45% of male and 72% of female student-athletes reported overwhelming anxiety at least once a month (NCAA, 2021). In addition to affecting an individual’s well-being, anxiety may negatively impact athletic performance (Edwards & Hardy, 1996). To better understand the impact of anxiety on athletic performance and develop effective interventions, both the intensity and direction of symptoms experienced must be considered. Incremental beliefs have been shown to be related to desirable performance outcomes and increased well-being in various populations (Danthony et al., 2020; Dweck, 2008), and may provide an effective intervention to interpret anxiety as more …

Development And Praxis Of Ph Drug Delivery System Contra Zika, Julissa Rodriguez

College of Graduate Studies: Theses & Dissertations

As the effects of global warming continue to spread throughout the world, another critical issue is slowly gaining attention in many urban tropical countries. The Emerging Infectious Diseases/ Pathogens list from the National Institutes of Health (NIH) listed the Zika virus as a potential pandemic threat. In 2015, an outbreak of Zika was noted in several South American countries until the spread reached an all-time high of 87 countries in 2017. During the outbreaks, adults affected were noted to have joint and muscle pains, fevers, and rashes. The worst cases reported would ultimately lead to Guillain-Barre symptoms. Still, pregnant women …

Executive Function & Self-Regulation In Preschool Age Children: Mindfulness Intervention, Larissa Lamarca

College of Graduate Studies: Theses & Dissertations

Executive function is an umbrella term used in psychology to define a number of cognitive processes that are important for success in daily life. Both self-awareness, being able to focus one’s thoughts and behavior, and self-regulation, being able to manage emotional and behavioral responses, are important components of executive function. Between three- and five-years of age, children begin developing such skills rapidly which are necessary for school readiness. This study recruited children participants from four local preschools to test for improvement in attention and self-regulatory skills. Two schools completed a mindful yoga activity for five consecutive days, while a second …

Demographic And Clinical Characteristics Predicting Missed Clinic Visits Among Patients Living With Hiv On Antiretroviral Treatment., Osaremhen Ikhile

College of Graduate Studies: Theses & Dissertations

Background: Patients living with HIV (PLHIV) often face challenges that contribute to missed clinical care which can impact their health outcomes.

Purpose: The primary objective of this study was to examine associations of clinical and sociodemographic factors such as age at the entrance into antiretroviral treatment (ART), rurality-urbanicity, biological sex, province of residence, and viral load with PLHIV’s tendency to miss scheduled visits for ART among persons living with HIV (PLHIV) in the Kinshasa and Haut-Katanga provinces of the Democratic Republic of Congo (DRC) between 2018 and 2019.

Methods: The study participants for this quantitative study were 5338 adults …

Endogenous Antioxidant Response And Lipid Peroxidation Levels In Muscle Tissue Of The Cownose Ray (Rhinoptera Bonasus), Bailey Berry

College of Graduate Studies: Theses & Dissertations

Oxidative stress is the result of an imbalance between reactive oxygen species (ROS) and antioxidant response within cells. ROS generation is a natural consequence of ETC activity within the mitochondria and is associated with aerobic metabolism. Oxidative stress data on elasmobranch muscle is limited, with previous studies primarily focusing on mammalian and teleost muscle. Elasmobranch skeletal muscle is generally composed of large proportions of white muscle fibers (anaerobic), low proportions of red muscle fibers (aerobic), and low proportions of pink muscle fibers (anaerobic). The cownose ray, a benthopelagic batoid species, presents an interesting model of study because it contains relatively …

New Women In The Old Dominion: Race And Gender In Progressive-Era Virginia, Rachel Scott

College of Graduate Studies: Theses & Dissertations

This thesis traces the development of Black and white Southern women’s pursuit of political power between the end of the Civil War and the ratification of the Nineteenth Amendment in 1920. Emancipation and the downfall of the antebellum planter aristocracy upset traditional Southern gender norms and opened new doors for women of both races in the political upheaval of Reconstruction. Though both Black and white women participated in the women’s club movement and joined women’s advocacy and charity groups following the Civil War, their work was distinctive both from each other and from other regional Progressive movements. The context of …

A Comparison Of Tung Oil Resin Reinforcements Using Fibrous Proteins And Peptides For Biological And Materials Applications, Parker L. Williams

College of Graduate Studies: Theses & Dissertations

In this study a tung oil based thermoset was reinforced with collagen and fibroin, and the resulting composites were analyzed for their physical properties. Tung tree seed oil is a great candidate for biobased polymer production because its triglycerides are primarily made of alpha eleostearic acid, a fatty acid with three conjugated carbon-carbon double bonds. These double bonds allow for mechanically strong crosslinking in the polymer. It has been observed that polymerized tung oil forms a gel. This issue has been addressed using divinylbenzene (DVB) and n-butyl methacrylate (BMA) as co-monomers. Similar bio-based polymers have been studied and tend to …

Carbon Dioxide And Steam Gasification Of Biochar: A Study On Mitigating Greenhouse Gas Emissions And Producing Syngas For Downline Synthesis Of Renewable Hydrocarbon Fuel, Parth Patel

College of Graduate Studies: Theses & Dissertations

This research explores the potential of mitigating CO₂ emissions from fossil fuel consumption and providing an alternative renewable source of energy by gasifying biochar with carbon dioxide (CO₂) and steam (H₂O). The utilization of captured carbon dioxide and wastewater in industrial sectors is considered to further contribute to the advancements in energy-efficient and low-emission technological solutions. This research evaluated the performance and kinetic parameters of biochar gasification through CO₂ and H₂O gasification using a stainless-steel fixed bed reactor. The operating parameters including biochar mass, reactor temperature, and inlet flow rate were varied. …

Comparative Analytics On Chilli Plant Disease Using Machine Learning Techniques, Sai Abhishta Roy Seelam

College of Graduate Studies: Theses & Dissertations

This thesis concerns the detection of diseases in chilli plants using machine learning techniques. Three algorithms, viz., Convolutional Neural Network (CNN), Recurrent Neural Network (RNN), and Multi-Layer Perceptron (MLP), and their variants have been employed. Chilli-producing countries, India, Mexico, China, Indonesia, Spain, the United States, and Turkey. India has the world’s largest chilli production of about 49% (according to 2020). Andhra Pradesh (Guntur) is the largest market in India, where their varieties are more popular for pungency and color. This study classifies five kinds of diseases that affect the chilli, namely, leaf spot, whitefly, yellowish, healthy, and leaf curl. A …

Extralegal Bias In The United States Military In Sexual Assault Cases, Taylor F. Blackston

College of Graduate Studies: Theses & Dissertations

By evaluating the case recommendations following a preliminary hearing from military sexual assault cases from fiscal years 2016-2018, this study aims to assess whether or not extralegal factors are influencing decisions of case recommendations of assigned convening authorities. Using secondary data from the Department of Defense’s annual reports on sexual assault in the United States military (n=5,171), this study aims to answer the following questions: Do extralegal factors contribute to convening authorities’ recommendations following Article 32 hearings? If so, what extralegal factors contribute to convening authority's decision on non-judicial hearing recommendations? The results of the following analyses identified several extralegal …

Assessment Of The Utilization Of Healthcare Services Specific To Pregnancy-Related Complications In Rural And Medically Underserved Georgia, Diana L. Aguirre

College of Graduate Studies: Theses & Dissertations

Maternal mortality is a continuing issue, with rates for the state of Georgia (3.7 per 100,000 live births) are higher than that of the US rates (2.9 per 100,000 per live births) (Centers for Disease Control and Prevention, National Center for Health Statistics, n.d., 2023). However, maternal mortality may not account for health disparity factors, apart from access to quality care and physiological issues, which is a factor in pregnancy-related deaths, Pregnancy-related death, as defined by the US Centers for Disease and Control (CDC), is a death of a woman that occurs during or within one year of pregnancy that …