Digital Commons Network^™

Noncovalent Chalcogen And Tetrel Bonding Interactions: Spectroscopic Study Of Halide-Carbonyl Sulfide Complexes, Christian T. Haakansson, Peter D. Watson, Timothy R. Corkish, Hayden T. Robinson, Allan J. Mckinley, Duncan A. Wild

Research outputs 2022 to 2026

Chalcogen and tetrel intermolecular bonding interactions formed between carbonyl sulfide and halide anions have been studied utilizing a combined experimental and theoretical approach. In particular, high-level CCSD(T) energetics and experimental anion photoelectron spectroscopy have been used in order to assign the dominant binding motif exhibited in these complexes. Halide anions solvated by multiple carbonyl sulfide molecules have also been investigated in order to ascertain the effect that additional binding partners has on the strength of the noncovalent interactions. The experimental and computational results support the main binding motif of carbonyl sulfide molecules with halide anions being chalcogen bonding, both in …

Using Primates As A Flagship Species In Marketing Campaigns: Effects On Proenvironmental Attitudes And Behavioral Intentions, Taylor Barber

All Master's Theses

Shade coffee plantations grow coffee under a canopy of trees and provide alternative habitats for many bird and primate species, known as agroecosystems, particularly in Latin America. The aim of the current project was to better educate the public about shade plantations and the positive effects they can have on conservation for primates. In addition, marketing tactics such as the presence of a shade plantation certification label and howler monkey images were assessed for their effects on consumer purchasing intentions as well as participant support for biodiversity and sustainability. Participants were recruited through the Department of Psychology’s research system at …

Staff Perceptions Of Responsibility And Implementation Of Cognitive Enrichment For Non-Human Primates In Zoo Settings, Ember Nevada Toth

All Master's Theses

In zoos, caregivers have considered enrichment as vital for their animal collections’ physical health and development. Since primate species are endangered and continue to decline in numbers in their natural habitat, zoos are steadily becoming the only places we can view and learn about them. In today’s zoos, cognitive enrichment—which falls into overlapping categories of enrichment and does not yet have a universally accepted definition—is either absent or inconsistently offered. Providing challenges to stimulate cognitive well-being has been found to influence the overall welfare of captive primates. Cognitive enrichment is considered very important according to zookeeper surveys but is not …

Intestinal Parasite Infection In Tibetan Macaques (Macac Thebetana) In Eastern China, Miarisoa Ramilison

All Master's Theses

Grooming is one of the crucial social behaviors of primate species that functions to remove parasites and maintain social bonds. Although, much data exists to support the idea that grooming is essential in forming and sustaining social bonds among individuals, there is a lack of information about the extent to which it removes ectoparasites or contributes to the spread of internal parasites. To fill this gap in the literature, we investigated the relationship between social contact behavior including grooming and intestinal parasite infection in Tibetan macaques (Macaca thibetana). This species lives at Mt. Huangshan in China. We used …

Chimpanzee (Pan Troglodytes) Skin Temperature During Resting Behavior At Different Ambient Temperatures, Benjamin Lake

All Master's Theses

Thermal imaging, as a tool in psychological research, has been used to distinguish mental states evoked by different environmental and social cues, with prior research across several species. Ambient temperature can affect the precision of the measures of body temperature, however, so we investigated a methodology for recording thermal imaging data in sanctuary-housed chimpanzees (Pan troglodytes) while they engaged in resting behavior across different ambient temperatures. Data were collected on 30 adult chimpanzees (16 females and 14 males) ranging in age from 21 to 53 years (Mage = 33.4 years, SD = 8.0). The chimpanzees lived in nine social groups …

Identifying Unexpected Inflammation Resulting From Drug-Drug Interactions, Keegan Jolly

All Master's Theses

Adverse drug events result in nearly 1.3 million emergency room visits per year in the United States of America. As much as 30% of these adverse events are a result of drug-drug interactions (DDI’s). There is a gap in knowledge concerning these DDI outcomes especially when it comes to inflammation. Inflammation is linked to a variety of chronic health conditions and non-infectious diseases such as cancer. Purpose: The goal of this study was to examine how many active drug ingredients from FDA’s Adverse Event Reporting System (FAERS), when combined (in groups of two or more), elicit an inflammatory response …

Zooarchaeology Of The Grissom Site (45kt301), Kittitas Valley, Washington, Steven Spencer

All Master's Theses

The Grissom site of central Washington was excavated 1967-71 by students and faculty from Central Washington State College. The site may represent a portion of a large, springtime intertribal gathering near Kittitas known as Che-lo-han, which involved camas root gathering, tribal councils, and social activities. Extensive radiocarbon dating places occupation ~1810-130 BP, based on 20 of 21 radiocarbon dates, with some stratigraphic mixing. Site materials have been subject to several previous studies, including a 2018 undergraduate pilot study by the author. Other fauna have not been reported outside of a 2012 fish analysis by Lubinski and Partlow. In consultation with …

Comparison Of Midwifery And Obstetric Care Among American Indian And Alaska Native Birthing People, Trista Barnhart

All Master's Theses

Despite making up only 3% of the U.S. population, American Indian/Alaska Native (AI/AN) individuals have the second highest maternal mortality rate in the United States, and the leading cause of maternal mortality and morbidity among AI/AN is postpartum hemorrhage. Many interventions used during childbirth have been identified as common causes of hemorrhage, such as lacerations of the vagina and cervix, operative vaginal deliveries, & augmentation of labor. Research has found that individuals who had midwives as their care providers have been found to be less likely to experience instrumental deliveries, epidural analgesia, labor augmentation, episiotomy or perineal lacerations, cesarean sections, …

Effect Of Soil Transplantation On Fungal Communities During Restoration, Thomas Luehl

All Master's Theses

Ectomycorrhizal fungi are integral parts of natural ecological systems that provide access to nutrients that would otherwise be unavailable by forming symbiotic relationships with plants. Relationships with ectomycorrhizal fungi are known to improve plant growth rate, increase plant resistance to pathogens, and improve survival. Current revegetation practices often ignore this relationship between fungi and plants. During the revegetation of the Interstate 90 Keechelus Lake wildlife overcrossing (Easton, WA), soil from forest sites adjacent to the restoration area were collected and used to inoculate a set of experimental plots to determine whether ectomycorrhizal fungi would become established in the plots and …

Manipulating Social Mobility To Measure Group Stability In Crickets, Anne Tolo

All Master's Theses

Dominance hierarchies are typically formed so that members of the hierarchy can benefit from residing in a social structure where conflict is decreased, and each individual obtains a rank within the group. Members can either actively seek out a higher social rank to maximize fitness or remain in a lower position to benefit from the protection of living in a group. This study was designed to manipulate the social stability typically exhibited within hierarchies by experimentally increasing social perturbance in groups of tropical house crickets, Gryllodes sigillatus. Rank changes per unit time were used as a measure to understand …

Estimating Evapotranspiration And Analyzing Soil Moisture And Heat Flux Parameters At Taneum Creek, Central Washington, Edward Vlasenko

All Master's Theses

In the past two decades, stream restoration work, primarily in the form of wood emplacement, has been undertaken in the Taneum Creek watershed, resulting in increased channel-floodplain connectivity. One of the goals of stream restoration was to boost dry season groundwater storage in the shallow floodplain aquifer. However, any gains in groundwater due to increased connectivity may be nullified by increased evapotranspiration (ET) losses because of denser floodplain vegetation. Within the floodplain aquifer budget, ET is a major flow of water out of the system and is not well quantified.

In order to quantify ET, a monitoring site was established …

An Evolution Of Gold Of The Swauk Mining District, Liberty Washington, Timothy Miller

All Master's Theses

The Swauk Mining District in Central Washington state is a historic mining community with active small scale mining dating back to the 1870’s. Gold has been mined from both lode and placer deposits. The placer deposits of Liberty area are unique due to the variety of gold textures, most notably are the nuggets of tangled masses of dendritic crystals and wire gold.

This research aims to determine the paragenic sequence that led to the formation of the lode and placer gold deposits using a combination of field work and laboratory analyses. Field work consisted of small-scale detailed mapping, recording of …

Forest Patrol Guards And Caregivers' Perceptions Of Wild Chimpanzee Populations Near Tacugama Chimpanzee Sanctuary, Sierra Leone, Virginia Sanchez

All Master's Theses

Relationships between humans and alloprimates, in particular between humans and chimpanzees (Pan troglodytes), have occurred for centuries. Chimpanzee discoveries by western philosophers date back to the 16^th and 17^th centuries, and their earliest accounts in West Africa suggest that chimpanzees interacted and lived in close contact with humans, thus shaping the way both species adapt to their environments. Humans occupy this same space along with chimpanzees, and we need them as much as they need us. The continued decline in wild populations of species such as chimpanzees can have negative impacts for biodiversity. Chimpanzees have a …

Effects Of Solar Arrays On Southwestern Desert Thermal Landscapes: Consequences For Terrestrial Ectotherms, Adrian Slade

All Master's Theses

Solar energy development in the Southwestern United States coincides with habitat for many sensitive and endemic species, including reptiles such as Mojave Fringe-toed Lizards and Mojave Desert Tortoises who are threatened by a warming and drying climate. While utility-scale solar energy reduces carbon emissions and should ultimately benefit climate-sensitive species, it locally destroys fragile desert habitat, displaces wildlife, and has been shown to incur local air and surface temperature changes. Mitigation-based translocation of wildlife species in the face of solar development yields mixed results, and the unprecedented pace of solar expansion in the desert highlights the need for alternative practices …

Hydrologic And Geomorphic Investigations Of Two Engineered Stream Crossings Under Interstate 90 In Washington State, Catherine Mast

All Master's Theses

The importance of stream restoration in providing a healthy ecosystem is widely recognized. The Washington State Department of Transportation (WSDOT) has implemented environmental changes to facilitate habitat restoration and wildlife passage along the Interstate 90 Highway (I-90) corridor where it passes over the Cascade Mountains. Prior to the I-90 corridor expansion, Price and Noble Creeks passed under the highway though culverts, limiting passage of aquatic species or wildlife below the highway. In 2019 the stream channel crossings were expanded, and the size/shape of these creeks were engineered to mirror what would be seen in a natural environment. Since construction, erosion …

Enhancing Crop Resilience By Harnessing The Synergistic Effects Of Biostimulants Against Abiotic Stress, Anam Asif, Maratab Ali, Muslim Qadir, Rajmohan Karthikeyan, Zora Singh, Ravjit Khangura, Francesco Di Gioia, Zienab F. R. Ahmed

Research outputs 2022 to 2026

Plants experience constant exposed to diverse abiotic stresses throughout their growth and development stages. Given the burgeoning world population, abiotic stresses pose significant challenges to food and nutritional security. These stresses are complex and influenced by both genetic networks and environmental factors, often resulting in significant crop losses, which can reach as high as fifty percent. To mitigate the effects of abiotic stresses on crops, various strategies rooted in crop improvement and genomics are being explored. In particular, the utilization of biostimulants, including bio-based compounds derived from plants and beneficial microbes, has garnered considerable attention. Biostimulants offer the potential to …

The Experience Of Meaningful Rehabilitation As Perceived By People With Chronic Pain: A Phenomenological Study, Katrina J. Liddiard, Annette J. Raynor, Helen Dejong, Cary A. Brown

Research outputs 2022 to 2026

BACKGROUND: People with chronic pain may seek rehabilitation to reduce pain and restore productivity and valued roles. Theoretically, a biopsychosocial approach makes rehabilitation more meaningful, however, the limited research on meaningful rehabilitation predominantly describes the perspective of therapists and researchers. The client's perspective of meaningfulness in rehabilitation is lacking. OBJECTIVE: To investigate the experience of meaningfulness in rehabilitation from the perspective of people with chronic pain. METHODS: Qualitative, semi-structured interviews were conducted with Australian adults who had chronic pain and recent experience of occupational therapy or physiotherapy. Sampling continued until thematic saturation occurred. Transcripts were coded and analyzed using theory-driven …

Microscale Pollen Release And Dispersal Patterns In Flowering Grass Populations, Carl A. Frisk, Godfrey P. Apangu, Geoffrey M. Petch, Simon Creer, Mary Hanson, Beverley Adams-Groom, Carsten A. Skjøth

Research outputs 2022 to 2026

Characterizing pollen release and dispersion processes is fundamental for knowledge advancement in ecological, agricultural and public health disciplines. Understanding pollen dispersion from grass communities is especially relevant due to their high species-specific allergenicity and heterogeneously distributed source areas. Here, we aimed to address questions concerning fine level heterogeneity in grass pollen release and dispersion processes, with a focus on characterizing the taxonomic composition of airborne grass pollen over the grass flowering season using eDNA and molecular ecology methods. High resolution grass pollen concentrations were compared between three microscale sites ( < 300 m apart) in a rural area in Worcestershire, UK. The grass pollen was modelled with local meteorology in a MANOVA (Multivariate ANOVA) approach to investigate factors relevant to pollen release and dispersion. Simultaneously, airborne pollen was sequenced using Illumina MySeq for metabarcoding, analysed against a reference database with all UK grasses using the R packages DADA2 and phyloseq to calculate Shannon's Diversity Index ( -diversity). The flowering phenology of a local Festuca rubra population was observed. We found that grass pollen concentrations varied on a microscale level, likely attributed to local topography and the dispersion distance of pollen from flowering grasses in local source areas. Six genera (Agrostis, Alopecurus, Arrhenatherum, Holcus, Lolium and Poa) dominated the pollen season, comprising on average 77 % of the relative abundance of grass species reads. Temperature, solar radiation, relative humidity, turbulence and wind speeds were found to be relevant for grass pollen release and dispersion processes. An isolated flowering Festuca rubra population contributed almost 40 % of the relative pollen abundance adjacent to the nearby sampler, but only contributed 1 % to samplers situated 300 m away. This suggests that most emitted grass pollen has limited dispersion distance and our results show substantial variation in airborne grass species composition over short geographical scales.

Utility Of Dna Methylation As A Biomarker In Ageing And Alzheimer’S Disease, Lidija Milicic, Tenielle Porter, Michael Vacher, Simon M. Laws

Research outputs 2022 to 2026

Epigenetic mechanisms such as DNA methylation have been implicated in a number of diseases including cancer, heart disease, autoimmune disorders, and neurodegenerative diseases. While it is recognized that DNA methylation is tissue-specific, a limitation for many studies is the ability to sample the tissue of interest, which is why there is a need for a proxy tissue such as blood, that is reflective of the methylation state of the target tissue. In the last decade, DNA methylation has been utilized in the design of epigenetic clocks, which aim to predict an individual’s biological age based on an algorithmically defined set …

Analysis Of Competencies Of The Engineering Research And Development For Technology Alumni Of The University Of The Philippines Los Banos, Paulyn G. Micor, Maria Ana T. Quimbo, John Erinorio M. Perez, Ruth Ortega-Dela Cruz

Journal of Public Affairs and Development

In the context of local and global workforce and people skills competitiveness, this study sought to determine whether the Engineering Research and Development for Technology - University of the Philippines Los Banos (ERDT-UPLB) alumni are competent in their chosen employment fields. Using the Likert scale of measurement, the skills, knowledge, and attitude competencies of engineering graduates were compared to the preferred competencies of employers and potential employers. Results showed no significant difference between the existing competencies of the ERDT alumni and the preferred competencies of prospective employers. Moreover, employers and potential employers have indicated a positive and favorable response to …

Using Intervention Analysis To Evaluate The Trends In Release Rates Of Recreational Fisheries Following Extensive Management Changes, Brett Crisafulli, Ebenezer Afrifa-Yamoah, Ute Mueller, Karina Ryan, David Fairclough, Johnny Lo

Research outputs 2022 to 2026

Changes to management of a fisheries resource are often required to ensure ongoing sustainability. However, such changes can sometimes lead to unintended effects such as increased release rates and associated post-release mortality. These effects may be highly variable between species and areas. Recreational fishing management changes were introduced on the west coast of Australia in 2009/10 to recover stocks of demersal scalefish. Key changes included reducing mixed species bag limits across management zones and increasing the minimum size limit for one species in some management zones. The restrictive catch limits resulted in increased release rates of key demersal species. However, …

Proteome Changes Resulting From Malting In Hordein-Reduced Barley Lines, Mahya Bahmani, Angéla Juhász, Utpal Bos, Mitchell G. Nye-Wood, Malcolm Blundell, Crispin A. Howitt, Michelle L. Colgrave

Research outputs 2022 to 2026

Hordeum vulgare L., commonly known as barley, is primarily used for animal feed and malting. The major storage proteins in barley are hordeins, known triggers of celiac disease (CD). Here, sequential window acquisition of all theoretical mass spectra (SWATH)-MS proteomics was employed to investigate the proteome profile of grain and malt samples from the malting barley cultivar Sloop and single-, double-, and triple hordein-reduced lines bred in a Sloop background. Using a discovery proteomics approach, 2688 and 3034 proteins were detected from the grain and malt samples, respectively. By utilizing label-free relative quantitation through SWATH-MS, a total of 2654 proteins …

Bidirectional Causality Between Immunoglobulin G N-Glycosylation And Metabolic Traits: A Mendelian Randomization Study, Xiaoni Meng, Weijie Cao, Di Liu, Isinta M. Elijah, Weijia Xing, Haifeng Hou, Xizhu Xu, Manshu Song, Youxin Wang

Research outputs 2022 to 2026

Although the association between immunoglobulin G (IgG) N-glycosylation and metabolic traits has been previously identified, the causal association between them remains unclear. In this work, we used Mendelian randomization (MR) analysis to integrate genome-wide association studies (GWASs) and quantitative trait loci (QTLs) data in order to investigate the bidirectional causal association of IgG N-glycosylation with metabolic traits. In the forward MR analysis, 59 (including nine putatively causal glycan peaks (GPs) for body mass index (BMI) (GP1, GP6, etc.) and seven for fasting plasma glucose (FPG) (GP1, GP5, etc.)) and 15 (including five putatively causal GPs for BMI (GP2, GP11, etc.) …

Coastal Groundwater-Dependent Ecosystems Are Falling Through Policy Gaps, Madeleine Dyring, Melissa M. Rohde, Ray Froend, Harald Hofmann

Research outputs 2022 to 2026

Coastal groundwater-dependent ecosystems (GDEs), such as wetlands, estuaries and nearshore marine habitats, are biodiversity hotspots that provide valuable ecosystem services to society. However, coastal groundwater and associated ecosystems are under threat from groundwater exploitation and depletion, as well as climate change impacts from sea-level rise and extreme flood and drought events. Despite many well-intentioned policies focused on sustainable groundwater use and species protection, coastal GDEs are falling through gaps generated by siloed policies and as a result, are declining in extent and ecological function. This study summarized then examined policies related to the management of coastal groundwater and connected ecosystems …

Orthodox Vs Paradox: The Roles Of Glycomics, Genetics And Beyond In Immunity, Immune Disorders And Glycomedicine, Wei Wang

Research outputs 2022 to 2026

The traditional ‘central dogma’ describes the flow of genetic information from DNA to RNA to protein. This process highlights the critical role of genes in living organisms. Nonetheless, ongoing immunological research coming to realize that emerging disciplines like glycomics and epigenetics are challenging the traditional viewpoint and extending the boundaries of the ‘central dogma’. . . .

Genetic And Immunological Insights Into Covid-19 With Acute Myocardial Infarction: Integrated Analysis Of Mendelian Randomization, Transcriptomics, And Clinical Samples, Zequn Zheng, Yueran Zhou, Yongfei Song, Pengxiang Ying, Xuerui Tan

Research outputs 2022 to 2026

Background: Globally, most deaths result from cardiovascular diseases, particularly ischemic heart disease. COVID-19 affects the heart, worsening existing heart conditions and causing myocardial injury. The mechanistic link between COVID-19 and acute myocardial infarction (AMI) is still being investigated to elucidate the underlying molecular perspectives. Methods: Genetic risk assessment was conducted using two-sample Mendelian randomization (TSMR) to determine the causality between COVID-19 and AMI. Weighted gene co-expression network analysis (WGCNA) and machine learning were used to discover and validate shared hub genes for the two diseases using bulk RNA sequencing (RNA-seq) datasets. Additionally, gene set enrichment analysis (GSEA) and single-cell RNA-seq …

Corticosteroid-Laba Inhalers Increase Phonation Threshold Pressure (Ptp) And Flow (Ptf) In Rabbits, Christopher Dromey, Kristine Tanner, Heidi J. Robison, Maya Elena Stevens, Ray M. Merrill, Julie Barkmeier-Kraemer, Ben Christensen

Faculty Publications

Objectives: This study examined the effects of a combination corticosteroid plus long-acting beta2-adrenergic agonist inhaler (IC) on rabbit phonation. Methods: White New Zealand male rabbits were assigned randomly to experimental and control groups (n = 11 per group). The experimental group received twice-daily doses of Advair HFA™ (fluticasone propionate 45 mcg and salmeterol 21 mcg) via a veterinary facemask with 1-way valve and spacer; the control group received aerosolized saline. After 8 weeks, animals were euthanized, larynges excised, frozen, and subsequently thawed and mounted on a standard bench apparatus. Phonation was elicited during 15 successive trials, and phonation threshold pressure …

Philippine Gabaldon Heritage School Buildings In The New Normal : Safety, Sustainability And Human Development Perspectives, Inero Ancho

Journal of Urban Culture Research

As the COVID-19 pandemic has impacted education, stakeholders find means to return to the New Normal through limited face-to-face learning. The Philippine Gabaldon School Buildings (GSBs) are seen as venues where this can happen. Built from 1908 –1915, GSBs were erected during the American colonial government. Republic Act 11194 mandates these buildings’ conservation. Due to its perceived astounding structure and cultural value, a qualitative study was conducted that delved into the existing publicly available documents related to the buildings, for in-depth analysis. The investigation highlights how GSBs potentially address the minimum health standards relevant to the pandemic and limited face-to-face …

Allele-Specific Quantification Of Human Leukocyte Antigen Transcript Isoforms By Nanopore Sequencing, Andrew E O Hughes, Maureen C Montgomery, Chang Liu, Eric T Weimer

2020-Current year OA Pubs

INTRODUCTION: While tens of thousands of HLA alleles have been identified by DNA sequencing, the contribution of alternative splicing to HLA diversity is not well characterized. In this study, we sought to determine if long-read sequencing could be used to accurately quantify allele-specific HLA transcripts in primary human lymphocytes.

METHODS: cDNA libraries were prepared from peripheral blood lymphocytes from 12 donors and sequenced by nanopore long-read sequencing. HLA reads were aligned to donor-specific reference sequences based on the known type of each donor. Allele-specific exon utilization was calculated as the proportion of reads aligning to each allele containing known exons, …

Updates In The Chronic Kidney Disease-Mineral Bone Disorder Show The Role Of Osteocytic Proteins, A Potential Mechanism Of The Bone-Vascular Paradox, A Therapeutic Target, And A Biomarker, Matthew J Williams, Sarah C White, Zachary Joseph, Keith A Hruska

2020-Current year OA Pubs

The chronic kidney disease-mineral bone disorder (CKD-MBD) is a complex multi-component syndrome occurring during kidney disease and its progression. Here, we update progress in the components of the syndrome, and synthesize recent investigations, which suggest a potential mechanism of the bone-vascular paradox. The discovery that calcified arteries in chronic kidney disease inhibit bone remodeling lead to the identification of factors produced by the vasculature that inhibit the skeleton, thus providing a potential explanation for the bone-vascular paradox. Among the factors produced by calcifying arteries, sclerostin secretion is especially enlightening. Sclerostin is a potent inhibitor of bone remodeling and an osteocyte …