Digital Commons Network^™

Characterization Of Vernal Pools Across National Parks In The Great Lakes Region, Samantha R. Kurkowski

Dissertations, Master's Theses and Master's Reports

Vernal pools are small, ephemeral wetlands that become inundated each spring and provide many ecosystem services to the surrounding upland forests. They also provide critical habitat for amphibians and invertebrates, as their temporary nature keeps them free of fish and reduces predator populations. As part of a mapping project, we collected baseline field data on vernal pool characteristics throughout five Great Lakes National Parks: Pictured Rocks National Lakeshore, Sleeping Bear Dunes National Lakeshore, Apostle Islands National Lakeshore, Isle Royale National Park, and Voyagers National Parks. Our goals were to characterize and assess how vernal pools vary within and across the …

Statistical Methods For Gene Selection And Genetic Association Studies, Xuewei Cao

Dissertations, Master's Theses and Master's Reports

This dissertation includes five Chapters. A brief description of each chapter is organized as follows.

In Chapter One, we propose a signed bipartite genotype and phenotype network (GPN) by linking phenotypes and genotypes based on the statistical associations. It provides a new insight to investigate the genetic architecture among multiple correlated phenotypes and explore where phenotypes might be related at a higher level of cellular and organismal organization. We show that multiple phenotypes association studies by considering the proposed network are improved by incorporating the genetic information into the phenotype clustering.

In Chapter Two, we first illustrate the proposed GPN …

Eutrophication On Twin Lakes: Applying A Phosphorus Budget And Shoreline Surveys To Assess Current & Historical Lake Health, Tyler K. Lemahieu

Dissertations, Master's Theses and Master's Reports

The Twin Lakes system, a series of lakes including Lake Gerald and Lake Roland, was historically categorized as oligotrophic but is now mesotrophic having experienced cultural eutrophication. A mass balance phosphorus budget model was constructed for the Twin Lakes system in Houghton, Co. Michigan for the 2022 stratified season. Additional spatial insights were gleaned through a survey of shoreline water quality, which corroborated the model results. The lakes currently experience substantial depletion of hypolimnetic dissolved oxygen during late summer stratification. A budget reveals approximately 22% (6.6 kg) of the current 29.5 kg total phosphorus inputs to Twin Lakes (during the …

Role Of Peripheral Contacts In Structure And Function Of C-Type Cytochromes, Dong Woo Shin

Dartmouth College Ph.D Dissertations

Structural perturbations influence many properties of proteins, but sequence variations are frequently observed in nature without perturbing the overall stability, fold, and function. In this thesis work, heme proteins cytochrome c and c₂ have been used to provide insight into the relationship between peripheral contacts and its function.

Recent studies with pathogenic cytochrome c mutations G41S and Y48H, and growth-inhibiting mutation K72A, have highlighted the importance of contacts between loops C and D in function of the protein. Characterization of these variants shows that loop D local stability and dynamics are altered upon loop C perturbation, influencing electron transfer, …

Identifying Spatial Heterogeneity In And Potential Biological Consequences Of River Thermal Environments, Keith Jeffrey Fritschie

Dartmouth College Ph.D Dissertations

Temperature is a nearly ubiquitous driver of ecological and evolutionary processes, especially for organisms whose body temperature changes with the environment. The overarching biological importance of environmental temperature and increased availability of temperature datasets have led to its inclusion in spatially continuous models of species’ performance, abundance, and distribution. However, variation in both environmental temperature and animal behavior at finer scales than captured by these models may reduce the accuracy of their scientific conclusions and conservation recommendations. Here I contribute to an improved understanding of thermal variability in river systems and its potential biological consequences for an iconic coldwater species, …

Accelerated Forgetting In People With Epilepsy: Pathologic Memory Loss, Its Neural Basis, And Potential Therapies, Sarah Ashley Steimel Phd

Dartmouth College Ph.D Dissertations

While forgetting is vital to human functioning, delineating between normative and disordered forgetting can become incredibly complex. This thesis characterizes a pathologic form of forgetting in epilepsy, identifies a neural basis, and investigates the potential of stimulation as a therapeutic tool. Chapter 2 presents a behavioral characterization of the time course of Accelerated Long-Term Forgetting (ALF) in people with epilepsy (PWE). This chapter shows evidence of ALF on a shorter time scale than previous studies, with a differential impact on recall and recognition. Chapter 3 builds upon the work in Chapter 2 by extending ALF time points and investigating the …

Advanced Tools For Time-Resolved Imaging Of Tissue Metabolism, Arthur Petusseau

Dartmouth College Ph.D Dissertations

Medical imaging is a crucial tool in diagnosing and treating diseases, and by far the most common set of tools used for point-of-care guidance between a physician and patient are optical devices. For example, visible light imaging has unique applications in endoscopy, dermatology, microscopy, and this range of devices utilize a wide range of optical signals for sensing the tissue. Fluorescence imaging has become a niche but growing methodology in medical diagnostics and treatment because of its simplicity and the ability to integrate reporter molecules, that can reveal detailed information about cellular structures, metabolism, signaling, and other biomolecular features of …

Inhibition Of Cal Pdz Domain Via Non-Canonical And Allosteric Interactions Reveals New Targeting Sites And Strategies, Nicholas Paul Gill

Dartmouth College Ph.D Dissertations

Significant therapeutic achievements have been made in the past decade towards increasing the pool of correctly folded F508del-CFTR and enhancing its activity at the apical membrane. Despite this success, there are still no FDA-approved therapeutics that directly target the post-endocytic stability of F508del-CFTR. Upon internalization, it is rapidly degraded through binding the CFTR-associated ligand (CAL) PDZ domain (CALP), which triggers its degradation in the lysosome. Our group and others have shown that inhibiting CAL increases the half-life of functional F508del-CFTR at the cell‑membrane and significantly enhances ion transport, suggesting that ‘stabilizer’ compounds, especially in conjunction with current CF therapeutics, would …

Mitochondrial Division: Synergizing In Mitochondrial Divisome, Ao Liu

Dartmouth College Ph.D Dissertations

Mitochondria are the energy factories of the cell. The dynamic nature of cells demands routine changes in mitochondrial morphology by fusion and division. The dynamin GTPase Drp1 is a central mitochondrial division protein, driving constriction of the outer mitochondrial membrane via oligomerization. At least four regulatory factors control Drp1 activity on the outer mitochondrial membrane (OMM): 1) receptor proteins (Mff, MiD49, MiD51, and Fis1); 2) actin filaments; 3) the mitochondrial phospholipid cardiolipin (CL); and 4) Drp1 post-translational modifications, of which two phosphorylation sites (S579 and S600) are the most well studied. However, the molecular mechanism of how these factors work …

Identification Of A Dna Polymerase Alpha Gene Sequence In Thermomyces Lanuginosus Fungus, Nathan Williams

Honors Program Theses

With cell growth being the main focus of our research, we chose to study DNA polymerase alpha. DNA polymerase alpha is likely to be involved in cell growth as it is the enzyme that starts DNA replication. Control of this enzyme might lead to decreased cell growth in cancer or increased cell growth in damaged spinal cord tissue. We chose the thermophilic fungus Thermomyces lanuginosus for the study of the enzyme due to the ability to compare the enzyme’s activity during fungal growth at different temperatures. Thermomyces exhibits rapid growth at high temperatures and slow growth at low temperatures. Studying …

Evaluation Of Methods To Analyze Microbial Composition In Soils, Joseph Correa

Honors Program Theses

DNA testing has become a vital tool for studying microbes in their natural environments, but there is a lack of standardization in the protocols for extracting environmental DNA. Cave systems provide a special and unique area to research how life thrives in extreme environments. Many parts of caves receive no sunlight and very little nutrients yet harbor microbial communities. Previous work in our lab developed a DNA extraction protocol that works with a wide range of microbial species and with different soil types. I updated this protocol by adding a pre-lysis rinse step and tested whether it performed better with …

Investigation Of Unexpected Purple Substance Extracted From Newsprint Paper, Raquel Wilhelm

Honors Program Theses

In an attempt to extract a green dye from newsprint advertisements, it was serendipitously discovered that a purple substance can be extracted from newsprint paper using methanol or acetic acid.1 Further investigations involving blank newsprint paper showed that the purple substance was a part of the paper itself rather than a component of the ink in the advertisements. Soxhlet extraction was used on the blank newspaper print to obtain the compound. Using different analytical instruments, characteristics of the compound were determined such as potential structures, functional groups, and percent composition. The ultimate goal was to determine the compound and its …

Language Development And Poverty: Considerations And Applications For Speech-Language Pathologists, Samantha Lynn Ehler

Honors Program Theses

Economics is one vital variable that can support or hinder the successful development and acquisition of language. A large body of research has shown that conditions of poverty often negatively impact a child’s language learning environment and brain development, resulting in poor language development and a high risk of speech and language disorders. Language development is a dynamic process that begins during the first years of life and is contingent on social interactions and safe predictable routines. A thorough examination of the relationship between familial financial standing and language development is the foundation for developing interventions that will minimize the …

Southern Adventist University Annual Report 2022-2023, Southern Adventist University

Annual Reports

The Annual Report for Southern Adventist University for 2022 to 2023.

Extraction Yield And Biological Activity Of Phycobiliproteins From Porphyridium Purpureum Using Atmospheric Cold Plasma Discharge And Jet Systems, Shaba Noore, Brijesh K. Tiwari, Anet R. Jambrak, Josipa Dukic, Janith Wanigasekara, James Curtin, Claudio Fuentes-Grunewald, Colm P. O’Donnell

Articles

Phycobiliproteins (PBPs) extracted from Porphyridium purpureum (P.p) have bioactive properties and are widely used as ingredients in nutraceutical and food applications. This study investigated the use of two cold plasma systems, namely cold plasma discharge system (CPDS) and cold plasma jet system (CPJS), for the aqueous extraction of PBPs from P. p. Three types of PBPs, namely phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC) were identified in the crude extracts obtained. The highest PBPs extraction yield (11.31 ± 1.02 mg/g DW P. p) was obtained from CPDS treated samples at 25 kV using N2 for 9 min. CPDS treatments were …

Novel Biorefinery Process For Extraction Of Laminarin, Alginate And Protein From Brown Seaweed Using Hydrodynamic Cavitation, Xianglu Zhu, Laura Healy, Rahel Suchintita Das, M.L. Bhavya, Shanmugapriya Karuppusamy, Da-Wen Sun, Colm O'Donnell, Brijesh K. Tiwari

Articles

This paper investigates a novel biorefinery process designed for the extraction of valuable compounds from brown seaweed Alaria esculenta using hydrodynamic cavitation (HDC). A two-stage process was developed to maximize the value of seaweed biomass by control of the processing time, solvent selection and HDC conditions to extract laminarin, alginate, mannitol and protein in a cascading manner, maximizing the value of seaweed biomass. After the first extraction stage using 0.1 M HCl, membrane ultrafiltration was employed to separate laminarin and mannitol. The purity of the laminarin and mannitol obtained was up to 86.57 ± 3.72 % and 40.49 ± 2.78 …

Recent Insights On The Conformational Changes, Functionality, And Physiological Properties Of Plant-Based Protein–Polyphenol Conjugates, Mehnaza Manzoor, Zaria Fozonne Ngabou Tchameni, Zuhaib F. Bhat, Amit K. Jaiswal, Sundeep Jaglan

Articles

Plant proteins are promising sources of nutrition with enhanced health benefits due to their interaction with bioactive compounds like polyphenols. These interactions can form protein–polyphenol complexes with diverse properties and applications in the food, nutraceutical, and pharmaceutical industries. To exploit the potential of these conjugates, it is essential to understand how they are formed, characterized, and delivered. Various techniques such as spectroscopy, thermodynamics, and molecular docking simulations can reveal the mechanism and outcomes of protein–polyphenol binding. Moreover, the surface modification of protein–polyphenol conjugation can enhance the pharmacological effects of different drug molecules and enable their targeted delivery to specific tissues …

Reinforcement Learning For Stock Option Trading, James Garza

ICT

Reinforcement learning has recently seen an increase in popularity due to its ability to learn from past experience and its capability of adapting quickly and effectively to new market conditions. This research will focus on reinforcement learning and its importance in trading stock options. Option traders can trade options with one of two option expirations: American or European style. This research will base the analysis on the American expiration style, considered more challenging in trading than the European expiration style. This could lead to the possibility of improving the current trading techniques. In addition, this research aims to understand the …

Bioactive Secondary Metabolites In Cave Dwelling Fungi, Jade Nuehring

Honors Program Theses

Water samples were collected in May 2022 from WTH lake and silent lake located in Wind Cave National Park, South Dakota. From the water samples collected, 8 pure fungal colonies were isolated. All samples underwent chemical extraction, growth inhibition testing, proton-nuclear magnetic resonance spectroscopy ( 1H NMR), and high-pressure liquid chromatography (HPLC). Three pure colonies also underwent DNA extraction, and sequencing of the ITS region for genus level identification. These samples were able to be identified to the genus level, and for these three samples were: Talaromyces, sp., Linnemannia, sp., and Gliomatsix, sp. The secondary metabolite that showed antimicrobial activity …

Transforming Lives For Eternity: 2023-2028 Strategic Plan, Southern Adventist University

Strategic Plans

Southern Adventist University's 2023-2028 Strategic Plan.

Neurodiversity And The Field Of Speech Language Pathology: A Literary Review Of The History, Common Practices, And Misconceptions, Wren Anne Repko

Honors Program Theses

Neurodiversity is a new and emerging topic within the field of communication sciences and disorders. This literature review describes what neurodiversity is alongside part of the history of neurodiversity, and then turns and looks at neurodiversity and neurodiverse-affirming care in the field of speech language pathology. This literature review also suggests an area of possible future research looking at undergraduate and graduate students' views of neurodiversity.

Invasive Buckthorn Mapping: A Uav-Based Approach Utilizing Machine Learning, Gis, And Remote Sensing Techniques In The Upper Peninsula Of Michigan, Vikranth Madeppa

Dissertations, Master's Theses and Master's Reports

An Invasive species is a species that is alien or non-native to the ecosystem which causes harm to economic, environmental, or human health (E.O. 13112 of Feb 3, 1999). Invasive species have posed a serious threat to ecosystems across the globe. These invasive species have impacts on the biodiversity and productivity of invaded forests. Remotely sensed data is a valuable resource for understanding and addressing issues related to invasive species. This study presents a novel approach for mapping the distribution of two invasive plant species, Common and Glossy Buckthorn, using unmanned aerial vehicles (UAVs), machine learning algorithms, geographic information systems …

The Rights Of Stateless Children Born From Cross-Border Reproductive Care, Carson Cook

Emory International Law Review

No abstract provided.

Recent Advances In Experimental Design And Data Analysis To Characterize Prokaryotic Motility, Megan M. Dubay, Jacqueline Acres, Max Riekeles, Jay Nadeau

Physics Faculty Publications and Presentations

Bacterial motility plays a key role in important cell processes such as chemotaxis and biofilm formation, but is challenging to quantify due to the small size of the individual microorganisms and the complex interplay of biological and physical factors that influence motility phenotypes. Swimming, the first type of motility described in bacteria, still remains largely unquantified. Light microscopy has enabled qualitative characterization of swimming patterns seen in different strains, such as run and tumble, run-reverse-flick, run and slow, stop and coil, and push and pull, which has allowed for elucidation of the underlying physics. However, quantifying these behaviors (e.g., identifying …

A Next Generation Science Standards (Ngss) Aligned 3rd Grade Weather Unit, Jennifer Hageman

Graduate Research Papers

This unit is the culmination of work on taking the NGSS for weather and climate and creating an inclusive unit for third grade students. At the time this unit was started, many teachers were taking the NGSS and creating units for the classrooms. This unit integrates math, literacy, and social studies standards to help explore the NGSS for weather and climate.

After completion of this unit it was given to two reviewers in the field of teaching. They read the unit and provided feedback for improvement. After review of the feedback it was noted that adaptations for students need to …

Interfacial Activity And Surface Pka Of Perfluoroalkyl Carboxylic Acids (Pfcas), Ruchi Patel, Luis E. Saab, Philip J. Brahana, Kalliat T. Valsaraj, Bhuvnesh Bharti

Faculty Publications

Perfluoroalkyl carboxylic acids (PFCAs) are widely used synthetic chemicals that are known for their exceptional stability and interfacial activity. Despite their industrial and environmental significance, discrepancies exist in the reported pKa values for PFCAs, often spanning three to four units. These disparities stem from an incomplete understanding of how pH influences the ionized state of PFCA molecules in the bulk solution and at the air-water interface. Using pH titration and surface tension measurements, we show that the pKa values of the PFCAs adsorbed at the air-water interface differ from the bulk. Below the equivalence point, the undissociated and dissociated forms …

Biogeography Of Special Metabolites Produced By Ant-Associated Bacteria, Katherine Hansen

Scripps Senior Theses

There is an urgent and growing need for novel antibiotics with the growing prevalence of antibiotic-resistant pathogens. Ecologically-guided discovery is a strategy that focuses on how and where specially evolved metabolites such as antibiotics are used in nature. This strategy reveals unique active molecules with potential for human use. An unexplored niche with great potential for antibiotic discovery exists with Southwestern fungus-farming Trachymyrmex ants and their Actinobacteria symbionts. In exchange for nutrients from the ant, the ants’ bacterial symbiont produces antifungal molecules that protect the ants’ fungal cultivar from invasion by pathogenic microorganisms. They also produce antibacterial molecules to protect …

Developmental Consequences Of Hatching Asynchrony In Boat-Tailed Grackles (Quiscalus Major), Emily Radner

Scripps Senior Theses

Boat-tailed grackles (Quiscalus major) typically lay clutches of three eggs and begin incubation before the clutch is complete, resulting in hatching asynchrony. To study the developmental consequences of hatching asynchrony on boat-tailed grackles, data loggers measured nest temperatures and were compared to ambient temperatures to see when incubation began, and it appeared to begin after the second egg is laid. The third laid egg had a significantly shorter lay to pip time and lay to hatch time than the first laid egg, highlighting the need to investigate the consequences of a shortened incubation time on development. To do …

Effects Of Child Sleep, Maternal Sleep, And Covid-19 Related Stressors On Maternal Stress, Jocelyn Chang

Scripps Senior Theses

Research has shown that poorer sleep quality can lead to increased stress and worse psychological functioning. In the parent-child dyad, sleep disturbances of both the child and parent can significantly affect parental functioning. However, in the context of parent-child relations, the contributions of maternal and child sleep to maternal outcomes are much less understood, especially in the context of a worldwide pandemic and global stressor such as COVID-19. Moreover, relations between stress and biological indicators have not been extensively studied, with most studies using self-reported measures of stress. This study aims to extend the findings of previous research by including …

Confronting Current Crises And Critical Challenges Of Climate Change, John Wang, Jeffrey Hsu, Yang Li, Vicky Ching Gu

Department of Information Management and Business Analytics Faculty Scholarship and Creative Works

Climate change heightens global warming and brings about impending risks for both human society and natural systems. Climate change is the greatest threat modern humans have ever faced. Pollutant discharges involve the global atmosphere and result in the challenge of global warming that must be solved before it results in irreversible damage. Current common threats of crises need common actions among all of us: every continent, every country, every community, and every common citizen. The earlier an action, the larger its impact. This review article scrutinizes the newest developments in this paramount important research area and provides directions for future …