Digital Commons Network^™

A Means Of Codifying Safety Cross-Training Knowledge Expectations For Biosafety Professionals, Robert J Emery, Scott J Patlovich, Kristin G King, Janelle Rios

Faculty, Staff and Student Publications

INTRODUCTION: The health and safety issues encountered by biosafety professionals in the daily conduct of their work is rarely limited solely to potentially infectious pathogens. A basic understanding of the other types of hazards inherent to laboratories is necessary. As such, management of the health and safety program at an academic health institution sought to ensure crosscutting competency for its technical staff, including staff members within the biosafety program.

METHODS: Using a focus group approach, a team of safety professionals from a variety of specialties developed a list of 50 basic health and safety items that any safety specialist should …

Delphi Initiative For Early-Onset Colorectal Cancer (Direct) International Management Guidelines, Giulia Martina Cavestro, Alessandro Mannucci, Francesc Balaguer, Heather Hampel, Sonia S Kupfer, Alessandro Repici, Andrea Sartore-Bianchi, Toni T Seppälä, Vincenzo Valentini, Clement Richard Boland, Randall E Brand, Tineke E Buffart, Carol A Burke, Riccardo Caccialanza, Renato Cannizzaro, Stefano Cascinu, Andrea Cercek, Emma J Crosbie, Silvio Danese, Evelien Dekker, Maria Daca-Alvarez, Francesco Deni, Mev Dominguez-Valentin, Cathy Eng, Ajay Goel, Josè G Guillem, Britt B S L Houwen, Charles Kahi, Matthew F Kalady, Fay Kastrinos, Florian Kühn, Luigi Laghi, Andrew Latchford, David Liska, Patrick Lynch, Alberto Malesci, Gianluca Mauri, Elisa Meldolesi, Pål Møller, Kevin J Monahan, Gabriela Möslein, Caitlin C Murphy, Karlijn Nass, Kimmie Ng, Cristina Oliani, Enrico Papaleo, Swati G Patel, Marta Puzzono, Andrea Remo, Luigi Ricciardiello, Carla Ida Ripamonti, Salvatore Siena, Satish K Singh, Zsofia K Stadler, Peter P Stanich, Sapna Syngal, Stefano Turi, Emanuele Damiano Urso, Laura Valle, Valeria Stella Vanni, Eduardo Vilar, Marco Vitellaro, Yi-Qian Nancy You, Matthew B Yurgelun, Raffaella Alessia Zuppardo, Elena M Stoffel, Associazione Italiana Familiarità Ereditarietà Tumori, Collaborative Group Of The Americas On Inherited Gastrointestinal Cancer, European Hereditary Tumour Group, And The International Society For Gastrointestinal Hereditary Tumours

Faculty, Staff and Student Publications

BACKGROUND & AIMS: Patients with early-onset colorectal cancer (eoCRC) are managed according to guidelines that are not age-specific. A multidisciplinary international group (DIRECt), composed of 69 experts, was convened to develop the first evidence-based consensus recommendations for eoCRC.

METHODS: After reviewing the published literature, a Delphi methodology was used to draft and respond to clinically relevant questions. Each statement underwent 3 rounds of voting and reached a consensus level of agreement of ≥80%.

RESULTS: The DIRECt group produced 31 statements in 7 areas of interest: diagnosis, risk factors, genetics, pathology-oncology, endoscopy, therapy, and supportive care. There was strong consensus that …

Metabolomic Markers Of Ultra-Processed Food And Incident Ckd, Donghan Su, Jingsha Chen, Shutong Du, Hyunju Kim, Bing Yu, Kari E Wong, Eric Boerwinkle, Casey M Rebholz

Faculty, Staff and Student Publications

BACKGROUND: High ultra-processed food consumption is associated with higher risk of CKD. However, there is no biomarker for ultra-processed food, and the mechanism through which ultra-processed food is associated with CKD is not clear. Metabolomics can provide objective biomarkers of ultra-processed food and provide important insights into the mechanisms by which ultra-processed food is associated with risk of incident CKD. Our objective was to identify serum metabolites associated with ultra-processed food consumption and investigate whether ultra-processed food-associated metabolites are prospectively associated with incident CKD.

METHODS: We used data from 3751 Black and White men and women (aged 45-64 years) in …

Adult And Regenerating Planarians Respond Differentially To Chronic Drug Exposure, Kevin Bayingana , '23, Danielle Ireland, Elizabeth Rosenthal , '23, Christina Rabeler, Eva-Maria S. Collins

Biology Faculty Works

There is a lack of data on the effects of chronic exposure to common drugs and stimulants on the developing nervous system. Freshwater planarians have emerged as a useful invertebrate model amenable to high-throughput behavioral phenotyping to assay chemical safety in adult and developing brains. Here, we leverage the unique strength of the system to test in parallel for effects on the adult and developing nervous system, by screening ten common drugs and stimulants (forskolin, clenbuterol, LRE-1, MDL-12,330A, adenosine, caffeine, histamine, mianserin, fluoxetine and sertraline) using the asexual freshwater planarian Dugesia japonica. The compounds were tested up to 100 …

Prevalence, Genetic Characterization, And Antibiogram Of Salmonella Enterica Recovered From Buffalo Meat, Takwa Fathy, Amira Zakaria, Samir Abd-Elghany, Khalid Sallam

Mansoura Veterinary Medical Journal

Objective: This study aimed to determine the prevalence, virulence-associated genes and antimicrobial resistance of Salmonella species recovered from buffalo meat at Mansoura city in Egypt. Salmonella virulence genes were detected using polymerase chain reaction targeting invA, stn, and hilA genes.Design: Observational study.Samples: 120 samples.Procedures: A total of 120 buffalo meat samples were bacteriologically analyzed to isolate and characterize the Salmonella spp. and its virulence genes, in addition to their antimicrobial resistance.Results: Thirty (25%) out of 120-samples from buffalo meat were positive Salmonella spp. Out of the 191 phenotypically identified Salmonella isolates, only 58 strains were molecularly confirmed as Salmonella spp. …

Design And Synthesis Of New Acyl Urea Analogs As Potential Σ1r Selective Ligands, R. Thapa, R. Flores, Kwan H. Cheng, Bereket Mochona, Donald Sikazwe

Physics and Astronomy Faculty Research

In search of synthetically accessible open-chain analogs of PD144418 or 5-(1-propyl-1,2,5,6-tetrahydropyridin-3-yl)-3-(p-tolyl)isoxazole, a highly potent sigma-1 receptor (σ1R) ligand, we herein report the design and synthesis of sixteen arylated acyl urea derivatives. Design aspects included modeling the target compounds for drug-likeness, docking at σ1R crystal structure 5HK1, and contrasting the lower energy molecular conformers with that of the receptor-embedded PD144418—a molecule we opined that our compounds could mimic pharmacologically. Synthesis of our acyl urea target compounds was achieved in two facile steps which involved first generating the N-(phenoxycarbonyl) benzamide intermediate and then coupling it with the appropriate amines—weakly to strongly …

Sophisticated Vs. Rugged: Examining Gendered Brand Communication Styles And Social Media Engagement, Joanne T. Cao, Jamye Foster, Gallayanee S. Yaoyuneyong, Lacey K. Wallace

Journal of Applied Marketing Theory

Advertising and marketing managers understand that not all brands need to be on social media, but what is not clearly understood is consumers’ engagement expectations for brands that are on social media. Therefore, this study investigates the impact of brand personality, specifically sophisticated and rugged brands, and gendered communication styles on consumers’ expectations of social media engagement. A scenario-based experiment is performed to test the hypotheses using a mock brand. The main study consisted of a Qualtrics consumer panel, and a MANOVA was utilized to examine three components of social media engagement (consumption, contribution, and creation) on the two brand …

Precision Nephrology Identified Tumor Necrosis Factor Activation Variability In Minimal Change Disease And Focal Segmental Glomerulosclerosis., Laura H. Mariani, Sean Eddy, Fadhl M. Alakwaa, Phillip J. Mccown, Jennifer L. Harder, Viji Nair, Felix Eichinger, Sebastian Martini, Adebowale D. Ademola, Vincent Boima, Heather N. Reich, Jamal El Saghir, Bradley Godfrey, Wenjun Ju, Emily C. Tanner, Virginia Vega-Warner, Noel L. Wys, Sharon G. Adler, Gerald B. Appel, Ambarish Athavale, Meredith A. Atkinson, Serena M. Bagnasco, Laura Barisoni, Elizabeth Brown, Daniel C. Cattran, Gaia M. Coppock, Katherine M. Dell, Vimal K. Derebail, Fernando C. Fervenza, Alessia Fornoni, Crystal A. Gadegbeku, Keisha L. Gibson, Laurence A. Greenbaum, Sangeeta R. Hingorani, Michelle A. Hladunewich, Jeffrey B. Hodgin, Marie C. Hogan, Lawrence B. Holzman, J Ashley Jefferson, Frederick J. Kaskel, Jeffrey B. Kopp, Richard A. Lafayette, Kevin V. Lemley, John C. Lieske, Jen-Jar Lin, Rajarasee Menon, Kevin E. Meyers, Patrick H. Nachman, Cynthia C. Nast, Michelle M. O'Shaughnessy, Edgar A. Otto, Kimberly J. Reidy, Kamalanathan K. Sambandam, John R. Sedor, Christine B. Sethna, Pamela Singer, Tarak Srivastava, Cheryl L. Tran, Katherine R. Tuttle, Suzanne M. Vento, Chia-Shi Wang, Akinlolu O. Ojo, Dwomoa Adu, Debbie S. Gipson, Howard Trachtman, Matthias Kretzler

Manuscripts, Articles, Book Chapters and Other Papers

The diagnosis of nephrotic syndrome relies on clinical presentation and descriptive patterns of injury on kidney biopsies, but not specific to underlying pathobiology. Consequently, there are variable rates of progression and response to therapy within diagnoses. Here, an unbiased transcriptomic-driven approach was used to identify molecular pathways which are shared by subgroups of patients with either minimal change disease (MCD) or focal segmental glomerulosclerosis (FSGS). Kidney tissue transcriptomic profile-based clustering identified three patient subgroups with shared molecular signatures across independent, North American, European, and African cohorts. One subgroup had significantly greater disease progression (Hazard Ratio 5.2) which persisted after adjusting …

Genetic Disruption Of System Xc-Mediated Glutamate Release From Astrocytes Increases Negative-Outcome Behaviors While Preserving Basic Brain Function In Rat, Evan Hess, Sarah N. Kassel, Gregory Simandl, Nicholas Raddatz, Brian Maunze, Matthew M. Hurley, Michael Grzybowski, Jason Klotz, Aron Geurts, Qing-Song Liu, Sujean Choi, Robert C. Twining, David A. Baker

Biomedical Sciences Faculty Research and Publications

The importance of neuronal glutamate to synaptic transmission throughout the brain illustrates the immense therapeutic potential and safety risks of targeting this system. Astrocytes also release glutamate, the clinical relevance of which is unknown as the range of brain functions reliant on signaling from these cells hasn't been fully established. Here, we investigated system xc- (Sxc), which is a glutamate release mechanism with an in vivo rodent expression pattern that is restricted to astrocytes. As most animals do not express Sxc, we first compared the expression and sequence of the obligatory Sxc subunit xCT among major classes of vertebrate species. …

4th Circuit Panel Revives Preliminary Injunction To Let Transgender Girl Compete While West Virginia Case Is Pending, Arthur S. Leonard

Other Publications

No abstract provided.

Recent Advances In Centrifugal Spinning And Their Applications In Tissue Engineering, Shaik Merkatur Hakim Marjuban, Musfira Rahman, Syeda Sharmin Duza, Mohammad Boshir Ahmed, Dinesh K Patel, Md Saifur Rahman, Karen Lozano

Faculty, Staff and Student Publications

Over the last decade, researchers have investigated the potential of nano and microfiber scaffolds to promote wound healing, tissue regeneration, and skin protection. The centrifugal spinning technique is favored over others due to its relatively straightforward mechanism for producing large quantities of fiber. Many polymeric materials have yet to be investigated in search of those with multifunctional properties that would make them attractive in tissue applications. This literature presents the fundamental process of fiber generation, and the effects of fabrication parameters (machine, solution) on the morphologies such as fiber diameter, distribution, alignment, porous features, and mechanical properties. Additionally, a brief …

Platelet-Derived Material For The Treatment Of Infrabony Periodontal Defects: A Systematic Review And Meta-Analysis, Armando U. Rivera D.D.S

The New York State Dental Journal

Aim: To compare the clinical outcomes of platelet-derived biomaterials, including recombinant human platelet-derived growth factors receptor BB (rhPDGF-BB), platelet-rich plasma (PRP) and platelet-rich fibrin (PRF), for treatment of periodontal infra-bony defects. Materials and Methods: A meta-analysis was conducted to compare pocket depth reduction (PRD) and clinical attachment gain (CAG) with and without rhPDGF-BB, PRP or PRF, for surgical treatments of periodontal infra-bony pockets. Eligible studies were randomized controlled clinical trials in adults with periodontal infra-bony defects. Results: Thirty-four randomized controlled trials involving 939 patients with a mean follow-up of 9.29(±2.49) months were included in this meta-analysis. All three platelet-derived materials …

Epigenome-Wide Association Study Reveals Cpg Sites Associated With Thyroid Function And Regulatory Effects On Klf9, Antoine Weihs, Layal Chaker, Tiphaine C Martin, Kim V E Braun, Purdey J Campbell, Simon R Cox, Myriam Fornage, Christian Gieger, Hans J Grabe, Harald Grallert, Sarah E Harris, Brigitte Kühnel, Riccardo E Marioni, Nicholas G Martin, Daniel L Mccartney, Allan F Mcrae, Christa Meisinger, Joyce B J Van Meurs, Jana Nano, Matthias Nauck, Annette Peters, Holger Prokisch, Michael Roden, Elizabeth Selvin, Marian Beekman, Diana Van Heemst, Eline P Slagboom, Brenton R Swenson, Adrienne Tin, Pei-Chien Tsai, Andre Uitterlinden, W Edward Visser, Henry Völzke, Melanie Waldenberger, John P Walsh, Anna Köttgen, Scott G Wilson, Robin P Peeters, Jordana T Bell, Marco Medici, Alexander Teumer

Faculty, Staff and Student Publications

Background:

Thyroid hormones play a key role in differentiation and metabolism and are known regulators of gene expression through both genomic and epigenetic processes including DNA methylation. The aim of this study was to examine associations between thyroid hormones and DNA methylation.

Methods:

We carried out a fixed-effect meta-analysis of epigenome-wide association study (EWAS) of blood DNA methylation sites from 8 cohorts from the ThyroidOmics Consortium, incorporating up to 7073 participants of both European and African ancestry, implementing a discovery and replication stage. Statistical analyses were conducted using normalized beta CpG values as dependent and log-transformed thyrotropin (TSH), free thyroxine, …

Global Epidemiology And Clinical Outcomes Of Carbapenem-Resistant Pseudomonas Aeruginosa And Associated Carbapenemases (Pop): A Prospective Cohort Study, Jinnethe Reyes, Lauren Komarow, Liang Chen, Lizhao Ge, Blake M Hanson, Eric Cober, Erica Herc, Thamer Alenazi, Keith S Kaye, Julia Garcia-Diaz, Lanjuan Li, Souha S Kanj, Zhengyin Liu, Jose M Oñate, Robert A Salata, Kalisvar Marimuthu, Hainv Gao, Zhiyong Zong, Sandra L Valderrama-Beltrán, Yunsong Yu, Paul Tambyah, Gregory Weston, Soraya Salcedo, Lillian M Abbo, Qing Xie, Karen Ordoñez, Minggui Wang, Martin E Stryjewski, Jose M Munita, David L Paterson, Scott Evans, Carol Hill, Keri Baum, Robert A Bonomo, Barry N Kreiswirth, Maria Virginia Villegas, Robin Patel, Cesar A Arias, Henry F Chambers, Vance G Fowler, Yohei Doi, David Van Duin, Michael J Satlin

Faculty, Staff and Student Publications

Background: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) is a global threat, but the distribution and clinical significance of carbapenemases are unclear. The aim of this study was to define characteristics and outcomes of CRPA infections and the global frequency and clinical impact of carbapenemases harboured by CRPA.

Methods: We conducted an observational, prospective cohort study of CRPA isolated from bloodstream, respiratory, urine, or wound cultures of patients at 44 hospitals (10 countries) between Dec 1, 2018, and Nov 30, 2019. Clinical data were abstracted from health records and CRPA isolates were whole-genome sequenced. The primary outcome was 30-day mortality from the day …

Interactions Of Bioactive Trace Metals In Shipboard Southern Ocean Incubation Experiments, Shannon M. Burns, Randelle M. Bundy, William Abbott, Zuzanna Abdala, Alexa R. Sterling, P. Dreux Chappell, Bethany D. Jenkins, Kristen N. Buck

Graduate School of Oceanography Faculty Publications

In the Southern Ocean, it is well-known that iron (Fe) limits phytoplankton growth. Yet, other trace metals can also affect phytoplankton physiology. This study investigated feedbacks between phytoplankton growth and dissolved Fe, manganese (Mn), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), and cadmium (Cd) concentrations in Southern Ocean shipboard incubations. Three experiments were conducted in September–October 2016 near the West Antarctic Peninsula: Incubations 1 and 3 offshore in the Antarctic Circumpolar Current, and Incubation 2 inshore in Bransfield Strait. Additions of Fe and/or vitamin B12 to inshore and offshore waters were employed and allowed assessment of metal (M) uptake …

The Pandemic Anxiety Inventory: A Validation Study, Irvin Sam Schonfeld, Tasmyn Prytherch, Mark Cropley, Renzo Bianchi

Publications and Research

The Pandemic Anxiety Inventory (PAI) assesses anxiety symptoms individuals attribute to the presence of a pandemic. We conducted this study of 379 British adults during the COVID-19 pandemic and found that the PAI exhibited excellent reliability and solid criterion validity. Pandemic anxiety was associated with reduced social support, anticipated life changes, financial strain, job loss, economic insecurity, and the hospitalization or death of a close friend or relative. Using correlational and bifactor analyses, we found that the PAI demonstrated solid convergent and discriminant validity. The findings suggest that the PAI can be used in research and clinical practice.

Tetrazole-Based Inhibitors Of The Bacterial Enzyme N-Succinyl-L,L-2,6-Diaminopimelic Acid Desuccinylase As Potential Antibiotics, Thomas Dipuma, Teerana Thabthimthong, Emma H. Kelley, Katherine Konczak, Megan Beulke, Claire A. Herbert, Thahani Shifna Habeeb Mohammad, Anna Starus, Boguslaw P. Nocek, Kenneth W. Olsen, Rick C. Holz Prof., Daniel P. Becker Ph.D.

Chemistry: Faculty Publications and Other Works

Based on a hit from a high-throughput screen, a series of phenyltetrazole amides was synthesized and assayed for inhibitory potency against DapE from Haemophilus influenzae (HiDapE). The inhibitory potency was modest but confirmed, with the most potent analog containing an aminothiazole moiety displaying an IC50 = 50.2 ± 5.0 μM. Docking reveals a potential binding mode wherein the amide carbonyl bridges both zinc atoms in the active site, and the tetrazole forms key hydrogen bonds with Arg330.

Assessing The Toxicity Of Aluminum Oxide Nanoparticles (Al2o3 Nps) Prepared By Laser Ablation Technique On Blood Components, Tuqa Sabah, Kareem H. Jawad, Nebras Essam

Journal of Applied Sciences and Nanotechnology

Along with its uses in a wide range of sectors, NP toxicity research was one of the fastest-growing areas of research, so the growing commercial applications brought aluminum oxide nanoparticles under the purview of toxicologists. This study shows the toxicity of Aluminum oxide Nanoparticles on blood components prepared using the pulsed laser ablation (PLA) Nd: YAG laser method. We confirmed the synthesis of aluminum Oxide nanoparticles by measuring color absorbance, UV-vis, scanning electron microscope techniques (SEM), and FTIR as characterization of Aluminum oxide Nanoparticles. The complete blood count (CBC) was used in the study of the toxicity effect of these …

Experimental Study And Quantum Calculations For Spiramycin And Isosorbide Dinitrate As Corrosion Inhibitors, Thekra Abd Alkarim, Khalida Al-Azawi, Rana Anaee

Journal of Applied Sciences and Nanotechnology

The investigation of the efficiency of two expired drugs, including Spiramycin and Isosorbide dinitrate, was done as corrosion inhibitors for aluminum in 0.1M HCl medium using the electrochemical method by Potentiostst at four temperatures (293, 303, 313, and 323 K) and 200 ppm as constant concentration for each drug. The results showed that these drugs gave good efficiencies equal to 98.836% and 94.285 % for Spiramycin and Isosorbide dinitrate at 293 K, respectively, by adsorption process on the metallic surface. They act as mixed-type inhibitors due to shifting corrosion potential either to active or noble direction. The corrosion data were …

Room Temperature 2-Fold Morphology Porous Silicon Impedance Matching Pesticide Sensor, Rasha Rashid, Alwan Alwan, Mohammed Mohammed

Journal of Applied Sciences and Nanotechnology

In this work, an investigation was conducted to study the effect of electrodes’ configuration of the double morphology macPSi on the performance of electrically matched impedance pesticides sensors. The purpose was to develop an efficient electrical sensor for the quantitative detection process of (Chlorpyrifos) pesticide in organic solvents. The effect of electrodes configuration of the front, front-back, and back coplanar electrodes on macPSi as based substrate was tested to select an optimum sensor metallization pathway.  The based efficient macPSi layer was fabricated using elevated laser irradiation power density at different periods. Morphological, optical, and electrical properties of the sensors were …

Study Of The Properties Of Ybco Superconductor Compound In Various Preparation Methods: A Short Review, Amir Abdul Hussein, Adi Abdul Hussein, Noor Hasan

Journal of Applied Sciences and Nanotechnology

Superconductors have entered into many applications and advanced technological fields, due to their excellent properties identified by zero resistance and expelling the magnetic field applied to them. Superconductivity is a viable technology to prevent energy losses contributed by electrical resistivity. Also, the magnetic flux is repelled entirely out of the body of superconducting material which makes the Meissner Effect. High-Temperature Superconductors (HTS) have become the focus of researchers and scientists. This is because it uses liquid nitrogen "LN" in cooling, which gives it significant critical temperatures compared to traditional materials based on liquid helium "LHe" in cooling. From this point …

Antioxidant Properties Of Galangin With Β-Cyclodextrin: An In Vitro And In Vivo, Zainab Abbas, Ghassan Sulaiman, Majid Jabir, Hamdoon Mohammed, Salman Mohammed

Journal of Applied Sciences and Nanotechnology

Galangin, a non-toxic phytochemical, is known to have a variety of therapeutic uses. This study looked into the role of inclusion complexes of galangin/β-cyclodextrin in increasing antioxidant activity over pure galangin. The role of this inclusion complex in increasing antioxidant activity in comparison to pure galangin. In this study, hydrogen peroxide assays were used in vitro. Galangin demonstrated concentration-dependent scavenging action in the 2-50 µg mL-1 range, with the highest level of activity possible 92.00% at 50 µg mL-1. In pure galangin, a decrease of 85.00% was observed. The ferric thiocyanate lipoperoxidation method was clarified by using galangin and galangin/β-cyclodextrin …

Biosynthesis And Optimization Of Polyhydroxyalkanoate (Pha) Production By Acinetobacter Lwoffii Isolate, Karrar Hameed, Iman Gatea, Amal Hussein

Journal of Applied Sciences and Nanotechnology

Polyhydroxyalkanoate (PHA) is an eco-friendly polymer that has various important biomedical uses, as well as biodegradability, drug delivery, and medical implants. It is a kind of polyester synthesized by various microorganisms as energy reserve material under inappropriate conditions. This study focused on testing waste cooking oil as the source of carbon to optimize PHA production. The tests included the most important environmental components within the culture medium affecting the growth of the bacterial isolate under the experiment and its ability to produce a polymer. Results revealed that the cultivation of Acinetobacter lwoffii, pre-isolated from hydrocarbon contaminated soil, under optimum conditions …

Oncolytic Ndv-Amha1 Strain Effective Against Glioblastoma Cancer Cells, Zahraa Kadhim, Ghassan Sulaiman, Ahmed Al-Shammari, Hamdoon Mohammed, Salman Mohammed

Journal of Applied Sciences and Nanotechnology

Glioblastoma multiforme (GBM) is one of the most life-threatening types of cancer that is difficult to treat. The search for effective yet safe therapy is progressing and non-conventional therapies such as using viruses as a smart and selective agent against cancer are promising. The aim of the study was the presence of a reliable method to use Newcastle disease virus (NDV) as an oncolytic agent against GBM, which attempted to propagate the NDV in laboratory experiments. Ahmed Majeed Hamza Al-Shammari-1 (AMHA1) attenuated strain of NDV was propagated in chicken embryos. The virus's tittered in Vero-slamed cells to determine the infective …

Study Of The Effects Of Solution Types On Concentration Of Iron Oxide By Pulsed Laser Ablation In Liquid, Rusul Al-Obaidy, Adawiya Haider, Sharafaldin Al-Musawi, Norhana Arsad

Journal of Applied Sciences and Nanotechnology

In this work, the development of nanosystems by Pulsed Laser Ablation in Liquid (PLAIL) is of considerable importance to expand their biomedical applications, such as drug delivery. In the present study, we focus on the conditions of the preparation Nd: YAG laser wavelength 266 nm and two different laser fluency (10,28) J/cm2 to control the concentration and size stability of superparamagnetic iron oxide nanoparticles (SPION) prepared by PLAIL. The characteristics of SPION are investigated by energy-dispersive X-ray spectroscopy (EDX) spectra which showed strong peaks of Fe and O. Magnetic characteristics of iron oxide nanoparticles indicated superparamagnetic properties of SPION and …

Study Of The Physicochemical And Microbiological Properties Of Mozzarella Cheese Coated With Edible Casings Subsidized With Green Tea, Ihab Abdulhadi, Mahdi Shakak, Kifah Doosh

Journal of Applied Sciences and Nanotechnology

In this study, mozzarella cheese covered with edible biodegradable films made from whey proteins reinforced with green alcoholic extract was manufactured to reduce environmental impact from degradable plastics. Two types of mozzarella cheese were produced: the first unwrapped (control) and the second coated with whey proteins fortified with 3% green tea (envelope sample). The effect on prolonging storage life was evaluated by chemical and microbiological tests. The moisture content on the first day was 53.26% and 53.30% for the control and envelope samples, respectively. This percentage decreased significantly (P≤0.05) on the last day to 47% and 51.06%, respectively. The pH …

Extraction And Purification Of Pullulanase From Local Mushrooms, Dalal Bedan

Journal of Applied Sciences and Nanotechnology

Pullulanase is defined as an extracellular carbohydrase enzyme responsible for the hydrolysis of pullulan into maltotriose. Pullulanase can be extracted from Agaricus bisporus edible mushroom and concentrated by dialysis tube using Tris-HCl buffer. This enzyme was purified by different ratios of ammonium sulphate; the optimal ratio was 30%. It can be purified by ion exchange using a DEAE-cellulose column with a final yield of 5.25 and a purification fold of 4.38. Following Sephadex G-100 gel filtration with a yield of 2.60 and purification fold of 5.0, the total activity of the enzyme reached 160IU and the specific activity was 14.5 …

Role Of Microalgae In Environmental Biotechnology To Remove Heavy Metals, Muzhda Qader, Yahya Shekha

Journal of Applied Sciences and Nanotechnology

The objective of the study is to evaluate the role of microalgae in heavy metal remediation (Pb and Cd). Three microalgae species were used (Tetradesmus nygaardi, Scenedesmus quadricauda, and Coelastrella sp.) with four concentrations of both tested heavy metals (lead and cadmium) were, (5, 15, 35, and 50 ppm). Samples were analyzed every 4th day during the experimental study for 20 days. The result showed that during experimental days the lower concentration (5ppm) has the highest reduction percent for Pb and Cd by all microalgal species (Tetradesmus nygaardi, Scenedesemus quadricauda, and Coellastrella sp.). Whereas Coellastrella sp. had the highest efficiency …

Pattern And Trends Of Helicobacter Pylori Genotypes In Gastric Cancer: A Kenyan 8-Year Study, Priscilla Njenga, Allan Njau, Zahir Moloo, Gunturu Revathi, Evariste Tshibangu, Yoshio Yamaoka

Pathology, East Africa

Background: Notable geographic and temporal variations in the prevalence and genotypes of Helicobacter pylori, in relation to gastric pathologies, have been observed; however, their significance and trends in African populations is scarcely described. The aim of this study, was to investigate the association of H. pylori and its respective CagA and vacuolating cytotoxin A (VacA) genotypes with gastric adenocarcinoma, and to describe the trends of H. pylori genotypes over an 8-year period (2012–2019).

Materials and methods: A total of 286 samples of gastric cancer cases and benign controls (one-to-one matching), from three main cities in Kenya, between …

Encouraging Or Guilt-Inducing? An Analysis Of Fitspiration Content And Its Effect On Body Image And Lifestyle Changes, Brenna Mazour

Honors Program: Senior Projects (Public)

More people are acquiring their nutrition and exercise information from social media accounts called fitspiration. Analyses of fitspiration content have found the focus to be on restrictive diets and excessive exercise that’s appearance driven. Although its intent is to inspire its consumers to change their lifestyle, many speculate that it brings upon negative body image and induces guilt. The Theory of Planned Behavior (TPB) has been used to determine why people take part in certain health behaviors, such as diets or exercise regimens posted on fitspiration. TPB shows how the characteristics of social media discourage people to use fitspiration as …