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2023

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Fostering A Sense Of Social Connectedness For Minoritized Students And Faculty In Online Learning, Shreya Vaishnav, Angélica Galván, Taewon Kim Jul 2023

Fostering A Sense Of Social Connectedness For Minoritized Students And Faculty In Online Learning, Shreya Vaishnav, Angélica Galván, Taewon Kim

Journal of Technology in Counselor Education and Supervision

Faculty and students with minoritized identities face additional systemic and social barriers such as disconnection from colleagues, inability to discern body language, and gap in communication. This paper addresses some of these barriers as well as provides practical strategies and resources for faculty and students to develop social connectedness in online learning environments. Strategies are provided for allies to support and foster change in online academic environments to build social connections and belongingness.


Incorporating Complexity Theory In Collaborative Educational Programs, Ximeng Chen Jul 2023

Incorporating Complexity Theory In Collaborative Educational Programs, Ximeng Chen

Northeast Journal of Complex Systems (NEJCS)

The field of education has witnessed an increasing trend of inter-organizational and inter-departmental collaborations and forming of networks. Collaborative educational programs have been implemented in a variety of ways. This paper proposes to understand and study collaborative educational programs through the lens of complexity theory and to utilize nonlinear research methods. This paper also proposes to connect the dots in the literature among complexity theory, collaborative educational programs, program evaluation, and alternative nonlinear research methods.


Descriptive Norms Caused Increases In Mask Wearing During The Covid-19 Pandemic, Samantha L. Heiman, Scott Claessens, Jessica D. Ayers, Diego Guevara Beltrán, Andrew Van Horn, Edward R. Hirt, Athena Aktipis, Peter M. Todd Jul 2023

Descriptive Norms Caused Increases In Mask Wearing During The Covid-19 Pandemic, Samantha L. Heiman, Scott Claessens, Jessica D. Ayers, Diego Guevara Beltrán, Andrew Van Horn, Edward R. Hirt, Athena Aktipis, Peter M. Todd

Psychological Sciences Faculty Publications and Presentations

Human sociality is governed by two types of social norms: injunctive norms, which prescribe what people ought to do, and descriptive norms, which reflect what people actually do. The process by which these norms emerge and their causal influences on cooperative behavior over time are not well understood. Here, we study these questions through social norms influencing mask wearing during the COVID-19 pandemic. Leveraging 2 years of data from the United States (18 time points; n = 915), we tracked mask wearing and perceived injunctive and descriptive mask wearing norms as the pandemic unfolded. Longitudinal trends suggested that norms and …


Increased Sirt3 Combined With Parp Inhibition Rescues Motor Function Of Sbma Mice, David R. Garcia Castro, Joseph R. Mazuk, Erin M. Heine, Daniel Simpson, R. Seth Pinches, Caroline Lozzi, Kathryn Hoffman, Phillip Morrin, Dylan Mathis, Maria V. Lebedev, Elyse Nissley, Kang Hoo Han, Tyler Farmer, Diane E. Merry, Qiang Tong, Maria Pennuto, Heather L. Montie Jul 2023

Increased Sirt3 Combined With Parp Inhibition Rescues Motor Function Of Sbma Mice, David R. Garcia Castro, Joseph R. Mazuk, Erin M. Heine, Daniel Simpson, R. Seth Pinches, Caroline Lozzi, Kathryn Hoffman, Phillip Morrin, Dylan Mathis, Maria V. Lebedev, Elyse Nissley, Kang Hoo Han, Tyler Farmer, Diane E. Merry, Qiang Tong, Maria Pennuto, Heather L. Montie

Department of Biochemistry and Molecular Biology Faculty Papers

Spinal and bulbar muscular atrophy (SBMA) is a neuromuscular disease with substantial mitochondrial and metabolic dysfunctions. SBMA is caused by polyglutamine (polyQ) expansion in the androgen receptor (AR). Activating or increasing the NAD+-dependent deacetylase, SIRT3, reduced oxidative stress and death of cells modeling SBMA. However, increasing diminished SIRT3 in AR100Q mice failed to reduce acetylation of the SIRT3 target/antioxidant, SOD2, and had no effect on increased total acetylated peptides in quadriceps. Yet, overexpressing SIRT3 resulted in a trend of motor recovery, and corrected TCA cycle activity by decreasing acetylation of SIRT3 target proteins. We sought to boost blunted SIRT3 activity …


Correlative Cryo-Fib And Cryo-Et Of Dendritic Spines And Synaptic Connections, Erik D Anderson, Christopher A Cronkite, Kimberley F Tolias, Steven J Ludtke Jul 2023

Correlative Cryo-Fib And Cryo-Et Of Dendritic Spines And Synaptic Connections, Erik D Anderson, Christopher A Cronkite, Kimberley F Tolias, Steven J Ludtke

Faculty, Staff and Students Publications

No abstract provided.


A Professional Development Program For Science Adjunct Faculty: The Mentoring-Learning Community (Mlc), Linda B. Purvis, Jason D. Lang, Julie A. Luft Jul 2023

A Professional Development Program For Science Adjunct Faculty: The Mentoring-Learning Community (Mlc), Linda B. Purvis, Jason D. Lang, Julie A. Luft

Georgia Journal of Science

Institutions of higher education have become increasingly dependent on adjunct faculty. These faculty members are often unfamiliar with current teaching strategies emphasizing an active learning approach. To support science adjunct faculty in learning about active learning, a professional development program was designed and implemented by the authors of this study, the Mentoring-Learning Community. The Mentoring-Learning Community program design was informed by literature regarding the use of professional development programs that focused on adjunct faculty. To determine the impact of this program, participants in the Mentoring-Learning Community were observed and interviewed over one semester. Mentoring-Learning Community participants transformed through all three …


Medical School Watercooler Newsletter - July 21, 2023, Lindsey Lyle Jul 2023

Medical School Watercooler Newsletter - July 21, 2023, Lindsey Lyle

Watercooler Newsletter

This is the July 21, 2023 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents include:

  • USA scientists develop new methodology to enhance study of cancer innervation
  • Ridgeway named assistant dean of student affairs at Whiddon College of Medicine
  • Register now for Takeover Tuesday
  • Bassam wins national award for distinguished service
  • USA mourns passing of Preud'Homme, Professor of pediatrics


Cerebrovascular Disease In Covid-19., James E Siegler, Savanna Dasgupta, Mohamad Abdalkader, Mary Penckofer, Shadi Yaghi, Thanh N Nguyen Jul 2023

Cerebrovascular Disease In Covid-19., James E Siegler, Savanna Dasgupta, Mohamad Abdalkader, Mary Penckofer, Shadi Yaghi, Thanh N Nguyen

Cooper Medical School of Rowan University Departmental Research

Not in the history of transmissible illnesses has there been an infection as strongly associated with acute cerebrovascular disease as the novel human coronavirus SARS-CoV-2. While the risk of stroke has known associations with other viral infections, such as influenza and human immunodeficiency virus, the risk of ischemic and hemorrhagic stroke related to SARS-CoV-2 is unprecedented. Furthermore, the coronavirus disease 2019 (COVID-19) pandemic has so profoundly impacted psychosocial behaviors and modern medical care that we have witnessed shifts in epidemiology and have adapted our treatment practices to reduce transmission, address delayed diagnoses, and mitigate gaps in healthcare. In this narrative …


Homeobox Transcription Factor Hbxa Influences Expression Of Over One Thousand Genes In The Model Fungus Aspergillus Nidulans, Sandesh S. Pandit, Jinfang Zheng, Yanbin Yan, Sophie Lorber, Olivier Puel, Sourabh Dhingra, Eduardo A. Espeso, Ana M. Calvo Jul 2023

Homeobox Transcription Factor Hbxa Influences Expression Of Over One Thousand Genes In The Model Fungus Aspergillus Nidulans, Sandesh S. Pandit, Jinfang Zheng, Yanbin Yan, Sophie Lorber, Olivier Puel, Sourabh Dhingra, Eduardo A. Espeso, Ana M. Calvo

Food for Health: Publications

In fungi, conserved homeobox-domain proteins are transcriptional regulators governing development. In Aspergillus species, several homeobox-domain transcription factor genes have been identified, among them, hbxA/hbx1. For instance, in the opportunistic human pathogen Aspergillus fumigatus, hbxA is involved in conidial production and germination, as well as virulence and secondary metabolism, including production of fumigaclavines, fumiquinazolines, and chaetominine. In the agriculturally important fungus Aspergillus flavus, disruption of hbx1 results in fluffy aconidial colonies unable to produce sclerotia. hbx1 also regulates production of aflatoxins, cyclopiazonic acid and aflatrem. Furthermore, transcriptome studies revealed that hbx1 has a broad effect on …


Excess Glucose Or Fat Differentially Affects Metabolism And Appetite-Related Gene Expression During Zebrafish Embryogenesis, Bridget Konadu, Carol K. Cox, Michael R. Garrett, Yann Gibert* Jul 2023

Excess Glucose Or Fat Differentially Affects Metabolism And Appetite-Related Gene Expression During Zebrafish Embryogenesis, Bridget Konadu, Carol K. Cox, Michael R. Garrett, Yann Gibert*

Cell and Molecular Biology Publications

Zebrafish embryos use their yolk sac reserve as the sole nutrient source during embryogenesis. The two main forms of energy fuel can be found in the form of glucose or fat. Zebrafish embryos were exposed to glucose or injected with free fatty acid/Triacylglycerol (FFA/TAG) into the yolk sac at 24 hpf. At 72 hpf, glucose exposed or FFA/TAG injected had differential effects on gene expression in embryos, with fat activating lipolysis and β-oxidation and glucose activating the insulin pathway. Bulk RNA-seq revealed that more gene expression was affected by glucose exposure compared to FFA/TAGs injection. Appetite-controlling genes were also …


The Racialized History Of Vice Policing, India Thusi Jul 2023

The Racialized History Of Vice Policing, India Thusi

Articles by Maurer Faculty

Vice policing targets the consumption and commercialization of certain pleasures that have been criminalized in the United States—such as the purchase of narcotics and sexual services. One might assume that vice policing is concerned with eliminating these vices. However, in reality, this form of policing has not been centered on protecting and preserving the moral integrity of the policed communities by eradicating vice. Instead, the history of vice policing provides an example of the racialized nature of policing in the United States. Vice policing has been focused on (1) maintaining racial segregation, (2) containing vice in marginalized communities, and (3) …


Neurotropic Manifestations As A Potential Risk Factor For Schizophrenia Following In Utero Exposure To Sars-Cov-2, Mir Ali R Talpur, Omar H. Elsayed, Rif S. El-Mallakh Jul 2023

Neurotropic Manifestations As A Potential Risk Factor For Schizophrenia Following In Utero Exposure To Sars-Cov-2, Mir Ali R Talpur, Omar H. Elsayed, Rif S. El-Mallakh

The University of Louisville Journal of Respiratory Infections

Background: COVID-19 infection is associated with neurologic and psychiatric morbidity that suggests a direct effect of the virus or secondary effect of an inflammatory process. These neuropsychiatric consequences may increase the likelihood of schizophrenia in the offspring of women who become infected with COVID-19 during their pregnancy.

Methods: We performed a directed narrative review of the literature focusing on the proposed pathophysiological processes that lead to schizophrenia and known pathological consequences of COVID-19 infection.

Results: Schizophrenia in adult offspring has been associated with maternal infections during pregnancy by a wide range of respiratory and neurotropic pathogens. Spikes in the incidence …


Gold Nanoparticles For Monitoring Of Mesenchymal Stem-Cell-Loaded Bioresorbable Polymeric Wraps For Arteriovenous Fistula Maturation, Allan John R Barcena, Joy Vanessa D Perez, Jossana A Damasco, Marvin R Bernardino, Erin Marie D San Valentin, Carleigh Klusman, Benjamin Martin, Andrea Cortes, Gino Martin Canlas, Huckie C Del Mundo, Francisco M Heralde, Rony Avritscher, Natalie Fowlkes, Richard R Bouchard, Jizhong Cheng, Steven Y Huang, Marites P Melancon Jul 2023

Gold Nanoparticles For Monitoring Of Mesenchymal Stem-Cell-Loaded Bioresorbable Polymeric Wraps For Arteriovenous Fistula Maturation, Allan John R Barcena, Joy Vanessa D Perez, Jossana A Damasco, Marvin R Bernardino, Erin Marie D San Valentin, Carleigh Klusman, Benjamin Martin, Andrea Cortes, Gino Martin Canlas, Huckie C Del Mundo, Francisco M Heralde, Rony Avritscher, Natalie Fowlkes, Richard R Bouchard, Jizhong Cheng, Steven Y Huang, Marites P Melancon

Faculty, Staff and Student Publications

Mesenchymal stem cell (MSC)-seeded polymeric perivascular wraps have been shown to enhance arteriovenous fistula (AVF) maturation. However, the wraps' radiolucency makes their placement and integrity difficult to monitor. Through electrospinning, we infused gold nanoparticles (AuNPs) into polycaprolactone (PCL) wraps to improve their radiopacity and tested whether infusion affects the previously reported beneficial effects of the wraps on the AVF's outflow vein. Sprague Dawley rat MSCs were seeded on the surface of the wraps. We then compared the effects of five AVF treatments-no perivascular wrap (i.e., control), PCL wrap, PCL + MSC wrap, PCL-Au wrap, and PCL-Au + MSC wrap-on AVF …


The Role Of Gut Dysbiosis In The Loss Of Intestinal Immune Cell Functions And Viral Pathogenesis, Farzaneh Fakharian, Siva Thirugnanam, David A. Welsh, Woong Ki Kim, Jay Rappaport, Kyle Bittinger, Namita Rout Jul 2023

The Role Of Gut Dysbiosis In The Loss Of Intestinal Immune Cell Functions And Viral Pathogenesis, Farzaneh Fakharian, Siva Thirugnanam, David A. Welsh, Woong Ki Kim, Jay Rappaport, Kyle Bittinger, Namita Rout

School of Medicine Faculty Publications

The gut microbiome plays a critical role in maintaining overall health and immune function. However, dysbiosis, an imbalance in microbiome composition, can have profound effects on various aspects of human health, including susceptibility to viral infections. Despite numerous studies investigating the influence of viral infections on gut microbiome, the impact of gut dysbiosis on viral infection and pathogenesis remains relatively understudied. The clinical variability observed in SARS-CoV-2 and seasonal influenza infections, and the presence of natural HIV suppressors, suggests that host-intrinsic factors, including the gut microbiome, may contribute to viral pathogenesis. The gut microbiome has been shown to influence the …


Phylogeny And Taxonomy Of Genus Physaria In North America, Binoshi Hettihewa Jul 2023

Phylogeny And Taxonomy Of Genus Physaria In North America, Binoshi Hettihewa

Theses

Physaria is a genus of ~108 species belonging to family Brassicaceae that is predominantly distributed in Western North America, but one species occurs in Arctic Russia and Northern Canada and several species occur in South America. Regardless of the vast number of species in the genus, the genus lacks a well-resolved phylogeny representing many taxa, partially because phylogeny reconstruction is complicated by the fact that many species of Physaria vary in chromosome numbers and ploidy levels. In chapter 1, we review how polyploids are formed and become established and summarize what is known about variation in chromosome number and ploidy …


Design And Optimization Of Devices And Architectures For Neuromorphic Silicon Photonics, Matthew Van Niekerk Jul 2023

Design And Optimization Of Devices And Architectures For Neuromorphic Silicon Photonics, Matthew Van Niekerk

Theses

Neuromorphic photonics is an exciting field at the intersection of neuroscience, integrated photonics, and microelectronics. To realize large-scale neural network-inspired systems, we must have a toolbox of linear and nonlinear operators. Here, we review state of the art for integrated photonic linear operators, which can perform linear operations using interference or wavelength-division-multiplexing (WDM) techniques, and nonlinear operators, which perform nonlinear operations with a combination of photodetection and modulation. We present some devices that fit into the needed toolbox. A new type of directional coupler, using skin depth engineering of electromagnetic waves, suppresses optical crosstalk and eliminates the need for waveguide …


Comparison Of Dna Vaccines With As03 As An Adjuvant And An Mrna Vaccine Against Sars-Cov-2, Praveen Neeli, Dafei Chai, Xu Wang, Navid Sobhani, George Udeani, Yong Li Jul 2023

Comparison Of Dna Vaccines With As03 As An Adjuvant And An Mrna Vaccine Against Sars-Cov-2, Praveen Neeli, Dafei Chai, Xu Wang, Navid Sobhani, George Udeani, Yong Li

Faculty, Staff and Students Publications

Emerging variants of SARS-CoV-2 call for frequent changes in vaccine antigens. Nucleic acid-based vaccination strategies are superior as the coding sequences can be easily altered with little impact on downstream production. mRNA vaccines, including variant-specific boosters, are approved for SARS-CoV-2. Here, we tested the efficacy of DNA vaccines against the SARS-CoV-2 Spike aided by the AddaS03 adjuvant using electroporation and compared their immunogenicity with an approved mRNA vaccine (mRNA-1273). DNA vaccination elicited robust humoral and cellular immune responses in C57BL/6 mice with Spike-specific antibody neutralization and T cells produced from 20 μg DNA vaccines similar to that from 0.5 μg …


Preclinical Characterization Of The Efficacy And Safety Of Biologic N‑001 As A Novel Pain Analgesic For Post‑Operative Acute Pain Treatment, Derek Allen, Samerender Nagam Hanumantharao, Rylie Mcdonell, Karen‑Amanda Irvine, Peyman Sahbaie, David Clark, Paul H. Blum Jul 2023

Preclinical Characterization Of The Efficacy And Safety Of Biologic N‑001 As A Novel Pain Analgesic For Post‑Operative Acute Pain Treatment, Derek Allen, Samerender Nagam Hanumantharao, Rylie Mcdonell, Karen‑Amanda Irvine, Peyman Sahbaie, David Clark, Paul H. Blum

School of Biological Sciences: Faculty Publications

Inhibition of actin remodeling in nerves modulates action potential propagation and therefore could be used to treat acute pain. N-001 is a novel protein analgesic engineered from several C. Botulinum toxins. N-001 targets sensory neurons through ganglioside GT1b binding and ADP-ribosylates G-actin reducing actin remodeling. The activity and efficacy of N-001 was evaluated previously in vitro and in a mouse inflammatory pain model. To assess the relevance of N-001 for treatment of acute post-surgical pain, the current study evaluated the efficacy of N-001 in a mouse hind-paw incision model by periincisional and popliteal nerve block administration combined with mechanical …


Homeobox Transcription Factor Hbxa Influences Expression Of Over One Thousand Genes In The Model Fungus Aspergillus Nidulans, Sandesh S. Pandit, Jinfang Zheng, Yanbin Yin, Sophie Lorber, Olivier Puel, Sourabh Dhingra, Eduardo A. Espeso, Ana M. Calvo Jul 2023

Homeobox Transcription Factor Hbxa Influences Expression Of Over One Thousand Genes In The Model Fungus Aspergillus Nidulans, Sandesh S. Pandit, Jinfang Zheng, Yanbin Yin, Sophie Lorber, Olivier Puel, Sourabh Dhingra, Eduardo A. Espeso, Ana M. Calvo

Department of Nutrition and Health Sciences: Faculty Publications

In fungi, conserved homeobox-domain proteins are transcriptional regulators governing development. In Aspergillus species, several homeobox-domain transcription factor genes have been identified, among them, hbxA/hbx1. For instance, in the opportunistic human pathogen Aspergillus fumigatus, hbxA is involved in conidial production and germination, as well as virulence and secondary metabolism, including production of fumigaclavines, fumiquinazolines, and chaetominine. In the agriculturally important fungus Aspergillus flavus, disruption of hbx1 results in fluffy aconidial colonies unable to produce sclerotia. hbx1 also regulates production of aflatoxins, cyclopiazonic acid and aflatrem. Furthermore, transcriptome studies revealed that hbx1 has a broad effect on the A. flavus …


Itag An Optimized Imid-Induced Degron For Targeted Protein Degradation In Human And Murine Cells, Habib Bouguenina, Stephanos Nicolaou, Yann-Vaï Le Bihan, Elizabeth A Bowling, Cheyenne Calderon, John J Caldwell, Brinley Harrington, Angela Hayes, P Craig Mcandrew, Costas Mitsopoulos, Fernando Jr Sialana, Andrea Scarpino, Mark Stubbs, Arjun Thapaliya, Siddhartha Tyagi, Hannah Z Wang, Francesca Wood, Rosemary Burke, Florence Raynaud, Jyoti Choudhary, Rob L M Van Montfort, Amine Sadok, Thomas F Westbrook, Ian Collins, Rajesh Chopra Jul 2023

Itag An Optimized Imid-Induced Degron For Targeted Protein Degradation In Human And Murine Cells, Habib Bouguenina, Stephanos Nicolaou, Yann-Vaï Le Bihan, Elizabeth A Bowling, Cheyenne Calderon, John J Caldwell, Brinley Harrington, Angela Hayes, P Craig Mcandrew, Costas Mitsopoulos, Fernando Jr Sialana, Andrea Scarpino, Mark Stubbs, Arjun Thapaliya, Siddhartha Tyagi, Hannah Z Wang, Francesca Wood, Rosemary Burke, Florence Raynaud, Jyoti Choudhary, Rob L M Van Montfort, Amine Sadok, Thomas F Westbrook, Ian Collins, Rajesh Chopra

Faculty, Staff and Students Publications

To address the limitation associated with degron based systems, we have developed iTAG, a synthetic tag based on IMiDs/CELMoDs mechanism of action that improves and addresses the limitations of both PROTAC and previous IMiDs/CeLMoDs based tags. Using structural and sequence analysis, we systematically explored native and chimeric degron containing domains (DCDs) and evaluated their ability to induce degradation. We identified the optimal chimeric iTAG(DCD23 60aa) that elicits robust degradation of targets across cell types and subcellular localizations without exhibiting the well documented "hook effect" of PROTAC-based systems. We showed that iTAG can also induce target degradation by murine CRBN and …


Targeting Polyploid Giant Cancer Cells Potentiates A Therapeutic Response And Overcomes Resistance To Parp Inhibitors In Ovarian Cancer, Xudong Zhang, Jun Yao, Xiaoran Li, Na Niu, Yan Liu, Richard A Hajek, Guang Peng, Shannon Westin, Anil K Sood, Jinsong Liu Jul 2023

Targeting Polyploid Giant Cancer Cells Potentiates A Therapeutic Response And Overcomes Resistance To Parp Inhibitors In Ovarian Cancer, Xudong Zhang, Jun Yao, Xiaoran Li, Na Niu, Yan Liu, Richard A Hajek, Guang Peng, Shannon Westin, Anil K Sood, Jinsong Liu

Faculty, Staff and Student Publications

To understand the mechanism of acquired resistance to poly(ADP-ribose) polymerase inhibitors (PARPi) olaparib, we induced the formation of polyploid giant cancer cells (PGCCs) in ovarian and breast cancer cell lines, high-grade serous cancer (HGSC)-derived organoids, and patient-derived xenografts (PDXs). Time-lapse tracking of ovarian cancer cells revealed that PGCCs primarily developed from endoreplication after exposure to sublethal concentrations of olaparib. PGCCs exhibited features of senescent cells but, after olaparib withdrawal, can escape senescence via restitutional multipolar endomitosis and other noncanonical modes of cell division to generate mitotically competent resistant daughter cells. The contraceptive drug mifepristone blocked PGCC formation and daughter cell …


Differential Effects Of Bariatric Surgery And Caloric Restriction On Hepatic One-Carbon And Fatty Acid Metabolism, Arnon Haran, Michael Bergel, Doron Kleiman, Liron Hefetz, Hadar Israeli, Sarah Weksler-Zangen, Bella Agranovich, Ifat Abramovich, Rachel Ben-Haroush Schyr, Eyal Gottlieb, Danny Ben-Zvi Jul 2023

Differential Effects Of Bariatric Surgery And Caloric Restriction On Hepatic One-Carbon And Fatty Acid Metabolism, Arnon Haran, Michael Bergel, Doron Kleiman, Liron Hefetz, Hadar Israeli, Sarah Weksler-Zangen, Bella Agranovich, Ifat Abramovich, Rachel Ben-Haroush Schyr, Eyal Gottlieb, Danny Ben-Zvi

Faculty, Staff and Student Publications

Weight loss interventions, including dietary changes, pharmacotherapy, or bariatric surgery, prevent many of the adverse consequences of obesity, and may also confer intervention-specific benefits beyond those seen with decreased weight alone. We compared the molecular effects of different interventions on liver metabolism to understand the mechanisms underlying these benefits. Male rats on a high-fat, high-sucrose diet underwent sleeve gastrectomy (SG) or intermittent fasting with caloric restriction (IF-CR), achieving equivalent weight loss. The interventions were compared to


Genome-Wide Crispr-Cas9 Screen Analyzed By Slider Identifies Network Of Repressor Complexes That Regulate Trim24, Lalit R Patel, Sabrina A Stratton, Megan Mclaughlin, Patrick Krause, Kendra Allton, Andrés López Rivas, Daniela Barbosa, Traver Hart, Michelle C Barton Jul 2023

Genome-Wide Crispr-Cas9 Screen Analyzed By Slider Identifies Network Of Repressor Complexes That Regulate Trim24, Lalit R Patel, Sabrina A Stratton, Megan Mclaughlin, Patrick Krause, Kendra Allton, Andrés López Rivas, Daniela Barbosa, Traver Hart, Michelle C Barton

Faculty, Staff and Student Publications

TRIM24 is an oncogenic chromatin reader that is frequently overexpressed in human tumors and associated with poor prognosis. However, TRIM24 is rarely mutated, duplicated, or rearranged in cancer. This raises questions about how TRIM24 is regulated and what changes in its regulation are responsible for its overexpression. Here, we perform a genome-wide CRISPR-Cas9 screen by fluorescence-activated cell sorting (FACS) that nominated 220 negative regulators and elucidated a regulatory network that includes the KAP1 corepressor, CNOT deadenylase, and GID/CTLH E3 ligase. Knocking out required components of these three complexes caused TRIM24 overexpression, confirming their negative regulation of TRIM24. Our findings …


Single-Cell Sequencing Reveals The Landscape Of The Human Brain Metastatic Microenvironment, Qianqian Song, Jimmy Ruiz, Fei Xing, Hui-Wen Lo, Lou Craddock, Ashok K Pullikuth, Lance D Miller, Michael H Soike, Stacey S O'Neill, Kounosuke Watabe, Michael D Chan, Jing Su Jul 2023

Single-Cell Sequencing Reveals The Landscape Of The Human Brain Metastatic Microenvironment, Qianqian Song, Jimmy Ruiz, Fei Xing, Hui-Wen Lo, Lou Craddock, Ashok K Pullikuth, Lance D Miller, Michael H Soike, Stacey S O'Neill, Kounosuke Watabe, Michael D Chan, Jing Su

Faculty, Staff and Student Publications

Brain metastases is the most common intracranial tumor and account for approximately 20% of all systematic cancer cases. It is a leading cause of death in advanced-stage cancer, resulting in a five-year overall survival rate below 10%. Therefore, there is a critical need to identify effective biomarkers that can support frequent surveillance and promote efficient drug guidance in brain metastasis. Recently, the remarkable breakthroughs in single-cell RNA-sequencing (scRNA-seq) technology have advanced our insights into the tumor microenvironment (TME) at single-cell resolution, which offers the potential to unravel the metastasis-related cellular crosstalk and provides the potential for improving therapeutic effects mediated …


Intraoperative Cortical Localization Of Music And Language Reveals Signatures Of Structural Complexity In Posterior Temporal Cortex, Meredith J Mccarty, Elliot Murphy, Xavier Scherschligt, Oscar Woolnough, Cale W Morse, Kathryn Snyder, Bradford Z Mahon, Nitin Tandon Jul 2023

Intraoperative Cortical Localization Of Music And Language Reveals Signatures Of Structural Complexity In Posterior Temporal Cortex, Meredith J Mccarty, Elliot Murphy, Xavier Scherschligt, Oscar Woolnough, Cale W Morse, Kathryn Snyder, Bradford Z Mahon, Nitin Tandon

Faculty, Staff and Student Publications

Language and music involve the productive combination of basic units into structures. It remains unclear whether brain regions sensitive to linguistic and musical structure are co-localized. We report an intraoperative awake craniotomy in which a left-hemispheric language-dominant professional musician underwent cortical stimulation mapping (CSM) and electrocorticography of music and language perception and production during repetition tasks. Musical sequences were melodic or amelodic, and differed in algorithmic compressibility (Lempel-Ziv complexity). Auditory recordings of sentences differed in syntactic complexity (single vs. multiple phrasal embeddings). CSM of posterior superior temporal gyrus (pSTG) disrupted music perception and production, along with speech production. pSTG and …


Single-Cell Chromatin Accessibility Profiling Of Acute Myeloid Leukemia Reveals Heterogeneous Lineage Composition Upon Therapy-Resistance, Huihui Fan, Feng Wang, Andy Zeng, Alex Murison, Katarzyna Tomczak, Dapeng Hao, Fatima Zahra Jelloul, Bofei Wang, Praveen Barrodia, Shaoheng Liang, Ken Chen, Linghua Wang, Zhongming Zhao, Kunal Rai, Abhinav K Jain, John Dick, Naval Daver, Andy Futreal, Hussein A Abbas Jul 2023

Single-Cell Chromatin Accessibility Profiling Of Acute Myeloid Leukemia Reveals Heterogeneous Lineage Composition Upon Therapy-Resistance, Huihui Fan, Feng Wang, Andy Zeng, Alex Murison, Katarzyna Tomczak, Dapeng Hao, Fatima Zahra Jelloul, Bofei Wang, Praveen Barrodia, Shaoheng Liang, Ken Chen, Linghua Wang, Zhongming Zhao, Kunal Rai, Abhinav K Jain, John Dick, Naval Daver, Andy Futreal, Hussein A Abbas

Faculty, Staff and Student Publications

Acute myeloid leukemia (AML) is a heterogeneous disease characterized by high rate of therapy resistance. Since the cell of origin can impact response to therapy, it is crucial to understand the lineage composition of AML cells at time of therapy resistance. Here we leverage single-cell chromatin accessibility profiling of 22 AML bone marrow aspirates from eight patients at time of therapy resistance and following subsequent therapy to characterize their lineage landscape. Our findings reveal a complex lineage architecture of therapy-resistant AML cells that are primed for stem and progenitor lineages and spanning quiescent, activated and late stem cell/progenitor states. Remarkably, …


Blood Flow Restriction As A Potential Therapy To Restore Physical Function Following Covid-19 Infection, Isaac J. Wedig, John J. Durocher, John Mcdaniel, Steve Elmer Jul 2023

Blood Flow Restriction As A Potential Therapy To Restore Physical Function Following Covid-19 Infection, Isaac J. Wedig, John J. Durocher, John Mcdaniel, Steve Elmer

Michigan Tech Publications

Accumulating evidence indicates that some COVID-19 survivors display reduced muscle mass, muscle strength, and aerobic capacity, which contribute to impairments in physical function that can persist for months after the acute phase of illness. Accordingly, strategies to restore muscle mass, muscle strength, and aerobic capacity following infection are critical to mitigate the long-term consequences of COVID-19. Blood flow restriction (BFR), which involves the application of mechanical compression to the limbs, presents a promising therapy that could be utilized throughout different phases of COVID-19 illness. Specifically, we hypothesize that: 1) use of passive BFR modalities can mitigate losses of muscle mass …


A Review Of Black Psychology Courses Across United States’ Colleges And Universities: An Update Of Hicks And Ridley (1979), Kerry Lynn Jones Jul 2023

A Review Of Black Psychology Courses Across United States’ Colleges And Universities: An Update Of Hicks And Ridley (1979), Kerry Lynn Jones

Electronic Theses and Dissertations Archive

More than 40 years ago, Hicks and Ridley (1979) asserted the need for Black Studies in psychology across American colleges and universities. Their study is one of few, if not the only, that has examined the frequency and types of course offerings in Black Psychology. Thus, the purpose of this study is to evaluate the current state of Black Psychology course offerings and course types in United States’ colleges and universities. Participants were recruited from 121 United States’ four-year colleges and universities, which included 63 Historically Black Colleges and Universities (HBCUs) and 58 U.S. News 2023 Best Psychology schools. Participants …


Bighorn Sheep Respiratory Disease Surveillance Via Animal Behavior And Community Science, Sidney Brenkus Jul 2023

Bighorn Sheep Respiratory Disease Surveillance Via Animal Behavior And Community Science, Sidney Brenkus

Electronic Theses and Dissertations Archive

As humans continue to alter the environment, infectious diseases have become increasingly important challenges to wildlife populations and surveillance can play an important role in the management of such diseases. However, physiological surveillance is logistically and economically challenging, hampering our ability to detect disease incidence and spread. My work aimed to circumvent some of these challenges using an iconic species of North American wildlife as a model system: bighorn sheep (Ovis canadensis). For decades, Bighorn Sheep Respiratory Disease (BHSRD) has reduced lamb survival, decreased population growth and stability, and even led to local extinctions. As such, monitoring this disease is …


Socialist Ideals Of The New Woman In Weimar Germany, Hafife Montgomery Jul 2023

Socialist Ideals Of The New Woman In Weimar Germany, Hafife Montgomery

Electronic Theses and Dissertations Archive

Montgomery, Hafife. Ph.D. The University of Memphis. August, 2023. Socialist Ideals of the New Woman in Weimar Germany. Major Professor: Daniel L. Unowsky, Ph.D. This dissertation investigates the women’s magazines published by the Social Democratic Party of Germany (Sozialdemokratische Partei Deutschlands, SPD: Die Genossin, 1924-1933, and Frauenwelt, 1924-1933), the Communist Party of Germany (Kommunistische Partei Deutschlands, KPD: Die Kommunistin, 1919-1926), and its media mogul Willi Münzenberg (Der Weg der Frau, 1931-1933), as well as the SPD affiliated Arbeiterwohlfahrt (Workers’ Welfare: Arbeiterwohlfahrt, 1926-1933) during the Weimar Republic (1918-1933). I examine the organizations’ reasons for publishing women’s periodicals during a time of …