Digital Commons Network^™

Exploring Polyamorous Relationship Experiences Of Lesbian, Gay, Bisexual, And Pansexual Individuals, Jiale Man

Theses and Dissertations

Polyamory is a type of relationship under the consensual non-monogamy relationship umbrella that assumes the possibility, validity, and worthwhileness of maintaining romantic, sexual, and intimate relationships with multiple people simultaneously. In Western society, the prevalence and domination of mononormativity have led to the marginalization of relationships alternative to monogamy (i.e., polyamory). As a result, individuals in polyamorous relationships continue to experience bias, stigma, and discrimination at varying levels. With a rise in public interest in CNM in the past two decades, scientists and researchers have slowly started to examine various topics related to CNM, including its prevalence, public attitudes toward …

Exploring The Relationship Between The Epigenome And Metabolism In Mycn-Amplified Neuroblastoma, Krista M. Dalton

Theses and Dissertations

High risk neuroblastoma (NB) is responsible for nearly 15% of all cancer related deaths in the pediatric population. The transcription factor MYCN is the most studied oncogene in NB, automatically conferring high-risk and has been found to be difficult to target. One way that MYCN amplification in NB functions to support tumorigenicity is through altered metabolism, satisfying the demand for rapid proliferation by increasing the nutrient flux through biosynthetic pathways. We performed an unbiased screen on select metabolic targeted therapy combinations and correlated sensitivity with over 20 subsets of cancer. We found that MYCN-amplified NB is hypersensitive to the …

The Living Legends Of Sholoukh – شلوخ الأساطير Facial Scarification In Sudan, Ayah S. Elnour

Theses and Dissertations

Sholoukh — ritual face scarring—is a dying art form in Sudan.

Used to distinguish members of one tribe from another,

Sholoukh reflects the tribal pride of an individual. In the past,

sholoukh was seen as important as having a passport for a tribe

member, however, it was not always applied by choice. Using

just a razor blade, the wisest, most senior tribal elder would scar

the face of a child, initiating them into the tribe for life.

As this tribal art form fades away, the dwindling Sholoukh-bearers

have become living legends in Sudan. To honor them, I create

a collage …

“It's Just Not Enough”: Examining The Experiences Of School-Based Sexual Health Education And Conversation Engagement With Trusted Adults Among Black Boys In Richmond, Va, Sharyn A. Dougherty

Theses and Dissertations

The Centers for Disease Control and Prevention estimate that youth make up a quarter of the sexually active population, yet account for half of the 20 million new sexually transmitted infections in the United States.In 2021, youth age 15-19 living in Virginia’s central region, which includes the Richmond Metropolitan area, had the second highest number of chlamydia cases and fourth highest number of gonorrhea cases among all age groups, with the highest rates among Black youth. The aim of this study is to explore the experiences of Black youth in Richmond, VA with school-based sexual health education (SHE) and their …

Coming Out To Treatment: Identifying Facilitators & Characterizing Experiences Of Sexual And Gender Minoritized Individuals In Opioid Use Disorder Treatment Before And During The Covid-19 Pandemic, Jaclyn S. Sadicario

Theses and Dissertations

Sexual and Gender Minority (SGM) individuals are disproportionately impacted by substance use disorders vs. their non-SGM counterparts. Additionally, SGM folks face more barriers to access for treatment than others due to minority stressors including discrimination, internalized stigma, and marginalization related to their sex and gender minority statuses. It is important for researchers and treating providers alike to understand how these different factors are impacting the treatment of substance use disorders in SGM populations. With this in mind, this two phase study employed a mixed-methods approach to (1) describe the population of SGM folks enrolled in a MOUD treatment program with …

Studying Gene Function In Streptococcus Sanguinis Using Rna-Sequencing Analysis, Ahmed Ismail

Theses and Dissertations

The oral cavity hosts hundreds of bacterial species, amongst them are the Streptococci family. More specifically, S. sanguinis is a benign bacteria in the oral cavity to protect the tooth surface from S. mutans. which is a pathogen causing dental caries. While S. sanguinis is harmless in the oral cavity, it becomes pathogenic once entering the bloodstream and causing Infective Endocarditis (IE). To further study S. sanguinis and eventually develop an effective drug to treat IE caused by S. sanguinis, a gene expression study was conducted. In order to study gene expression, RNA-sequencing was conducted, and appropriate methods to remove …

Improving The Use Of Diffusing Wave Spectroscopy As A Multiphase Process Analytical Technology, Robert Emerson Mcmillin Iii

Theses and Dissertations

The study of multiphase formulations is a difficult task due to inhomogene- ity. Typical methods of study, such as microscopy, suffer from sampling drawbacks and are ill-equipped at probing the system in its native state. Additionally, more concentrated sys- tems, including optically-dense emulsions and suspensions, render common light-scattering techniques, such as dynamic light scattering (DLS), useless. Lastly, macroscopic phase track- ing or transmission intensity techniques can only obtain partial information about a system. Herein, the use of diffusing wave spectroscopy (DWS), a multiple scattering adjunct to DLS, to study optically dense multiphase systems is explored. Three different systems are exam- …

Fascism In Sci-Fi: "Mobilizing Passions" In Robert A. Heinlein's Starship Troopers, Alton C. Ayers

Theses and Dissertations

This thesis responds to criticism of Robert A. Heinlein’s Starship Troopers (1959) as a “fascist” novel by further investigating the claim through a close reading of the novel that applies political theory scholarship on fascism. Chapters I and II introduce the novel along with its general reception and controversy. These chapters consider the accusations of “fascism” given to the novel while at the same time understanding that a clear, exact definition of “fascism” has long been grappled with by scholars since the rise of the regimes in Nazi Germany and Fascist Italy. Chapters III and IV apply political theory to …

Supporting Students Who Are Deafblind To Increase Their Access To The General Education Curriculum Through Trained Interveners: A Mixed Methods Study, Ira Padhye

Theses and Dissertations

The purpose of this mixed-methods research study was to identify the educational trends of children and young adults who are deafblind and identify strategies to improve the outcomes through interviews of nationally qualified interveners who have received training in deafblind specific strategies. Secondary data from the 2017-2021 National Center on Deafblindness and interview transcripts were used for data analysis and then integrated to identify the supports students who are deafblind should access. Trends in age of identification, primary eligibility category, school settings and access to state standardized assessments showed a plateau and displayed no significant improvement in educational outcomes. Nationally …

The Cultural Diplomacy Of U.S. Peace Corps: Understanding The Role Of Peace Corps Volunteers As Strategic Geopolitical Assets, Corey B. Miles

Theses and Dissertations

Beginning in the Summer of 1961, the United States government has deployed volunteer humanitarians around the world to serve the mission of U.S. Peace Corps and has since contributed to communities in 143 partner countries while enjoying widespread popularity. Its mission of promoting world peace and friendship is pursued through three operational goals: technical training, cultural exportation, and cultural importation. Since 2016, with the election of U.S. President Donald Trump, and continuing through the Covid-19 pandemic, which produced the first global service interruption in agency history, the value of the Peace Corps service model has been fundamentally challenged. As a …

Anti-Immigration-Related Stressors And Latine Immigrant Well-Being: The Role Of Family & Community Resilience, Lisa S. Fuentes

Theses and Dissertations

Anti-immigrant rhetoric and policies have continued to adversely impact Deferred Action for Childhood Arrivals (DACA) and Temporary Protection Status (TPS) beneficiaries (Arevalo et al., 2015; Cleary, 2017; Li, 2015; Sangalang et al., 2018). There have been numerous attempts to revoke DACA, TPS, and other policies that temporarily protect immigrant families. Although the number of resilience literature has grown, more strength-based approaches are still needed. Additionally, given that familismo (familism) and collectivism are key cultural values for Latine families (Campos et al., 2019; Corona et al., 2017), it is imperative to explore family and community resilience among Latine immigrants to gain …

Effects Of Perpetrator And Victim Gender, Rape Myths, And Report Timing On Blame Attributions Following Sexual Assault, Katherine E. Kennon

College of Graduate Studies: Theses & Dissertations

Previous research examined the effects of victim gender, perpetrator gender, and rape myth acceptance on victim blaming attribution; however, fewer studies explore victim and perpetrator gender at the same time, and even fewer studies examined the relevance of factors like timing of reporting (immediate or delayed) or rurality. The primary purpose of the study, therefore, was to explore the effect of victim gender, perpetrator gender, and report timing (same day vs. six months later) on victim blaming attributions. The study also examined the role of rape myth acceptance on victim blame and compared levels of rape myth acceptance across participant …

Investigation Of Sneathia Vaginalis And The Host Antibody Response To S. Vaginalis Pore-Forming Toxin, Cpta, In Vivo, Zion T. Mccoy

Theses and Dissertations

Sneathia vaginalis, formerly Sneathia amnii, is a gram-negative vaginal species that is associated with pregnancy complications including preterm birth. The only virulence factor of S. vaginalis that has been characterized is the cytopathogenic toxin, component A (CptA), a pore-forming toxin that lyses human red blood cells and human epithelial cells and plays a role in traversal of the bacteria through fetal membranes. The overarching goal of this work was to examine the host immune response to CptA. To investigate the immune response to CptA, levels of anti-CptA IgM, IgA, and IgG in human mid-vaginal swab samples obtained during …

Totem Impulse, Tendai T. Mupita

Theses and Dissertations

In this reading, I explore the performative and complexities of indigenous mythologies, cosmologies, and epistemologies, through totems, folklore, architecture, and games. This paper extracts references from indigenous philosophies in thinking about form and image. These forms and images are my sculptures, my installations, and my lines.

Treponema Denticola Synthesizes C-Di-Amp And Encodes The Cdaa-Type Diadenylate Cyclase Cdaa, Claire R. O'Brien

Theses and Dissertations

Periodontitis is a form of oral disease characterized by dysbiosis of the oral microbiome, leading to inflammation, bone resorption, and in severe cases, entire tooth loss, affecting 42% of adults in the US. One of the bacteria most associated with periodontal disease progression is Treponema denticola (Td), an oral spirochete which inhabits the mouth in small quantities during health but which can dominate the biofilms that form during periodontal disease. The ability of Td to survive in a disease environment and contribute to the progression of disease requires the use of robust signaling networks. Analysis of Td cultures …

Characterization And Investigation Of Cold Atmospheric Plasma And Its Effects On Cancer Cell Biology, Thomas M. Ritrosky

Theses and Dissertations

Modern cancer treatment uses radiation therapy in over 50% of patient cases. It is an e↵ective way of treating tumors because the mechanisms of cell killing are well known through the damage that ionizing radiation does to DNA. The amount of radiation can be tracked through measuring the dose of the clinical photon or electron beam used. However, there are limitations in the usage of radiation therapy, for example, a tumor can create hypoxic areas that become radioresistant leading to complete ine↵ectiveness of further radiation treatment. This project looks into the application of cold atmospheric plasma as an adjuvant therapy …

Sense Of Belonging As Behaviorist Construct: “Doing Belonging” And Its Application For College Students With Disabilities, Chris Parthemos Phd

Theses and Dissertations

Sense of Belonging has been widely linked to academic performance, retention and persistence for college students. Students with disabilities are less likely to enroll than peers, and are more likely to drop out. Similar to other historically-marginalized groups, these students report lower sense of belonging than peers. Research with this population suggests that they may be seeking ways of building community and belonging at their universities which are self-driven. This notion of “doing belonging”, as it is described in the sociological literature, appears at first glance to be compatible with behaviorist approaches to student support. Although not without controversy, behavior …

Buckets In A Deep Well: Essays About Shakespeare’S History Play Characters And An Original Drama, Brian C. Bassett

Theses and Dissertations

Buckets in a Deep Well is a set of essays that examines various characters portrayed in Shakespeare’s history plays, as well as parallel figures in 20th and 21st century works. Each essay primarily focuses on a single character’s response to a power structure: Richard Gloucester to the Tudor England of his audience in Richard III; Richard Nixon’s campaign-produced version of himself to 20th century American democracy in “Checkers;” Falstaff to organized society in Henry IV, Part 1; Marlo Stanfield to the drug economy of early 2000’s Baltimore in The Wire; the Duke of York to the disrupted monarchy of The …

Formation Of Vasculogenic Mimicry: Role Of Melanoma Differentiation Associated Gene-9 In Human Melanoma, Jinkal Shah

Theses and Dissertations

Malignant melanoma (MM) is the most aggressive skin cancer and the most frequent skin disorder in Caucasians. MM is associated with aggressive and progressive disease states, leading to major cancer-related morbidity and mortality. Recent investigations identify an alternative pathway -vasculogenic mimicry (VM), which is considered a cancer hallmark that can independently facilitate tumor neovascularization by the formation of fluid-conducting and vascular endothelial cells. MM cells undergoing VM can dedifferentiate into numerous cellular phenotypes and acquire endothelial-like features, resulting in the formation of the de novo matrix-rich vascular-like networks, such as plasma and red blood cells. The co-generation of endothelial cells, …

Of Systems Thinking And Straw Men, Kate Klonick

Faculty Publications

(Excerpt)

In Content Moderation as Systems Thinking, Professor Evelyn Douek, as the title suggests, endorses an approach to the people, rules, and processes governing online speech as one not of anecdote and doctrine but of systems thinking. She constructs this concept as a novel and superior understanding of the problems of online-speech governance as compared to those existent in what she calls the “standard [scholarly] picture of content moderation.” This standard picture of content moderation — which is roughly five years old — is “outdated and incomplete,” she argues. It is preoccupied with anecdotal, high-profile adjudications in which platforms …

Get Out: Structural Racism And Academic Terror, Renee Nicole Allen

Faculty Publications

Released in 2017, Jordan Peele’s critically acclaimed film Get Out explores the horrors of racism. The film’s plot involves the murder and appropriation of Black bodies for the benefit of wealthy, white people. After luring Black people to their country home, a white family uses hypnosis to paralyze victims and send them to the Sunken Place where screams go unheard. Black bodies are auctioned off to the highest bidder; the winner’s brain is transplanted into the prized Black body. Black victims are rendered passengers in their own bodies so that white inhabitants can obtain physical advantages and immortality.

Like Get …

Hats For Sale: Efficiency, Economics, And Process Integrity, Elayne E. Greenberg

Faculty Publications

(Excerpt)

What are the ethical considerations for a mediator when a neutral is asked to be both the mediator and arbitrator on the same case? Some parties and their lawyers opt to select one neutral to serve as both the mediator and arbitrator on the same case, believing it will be a more efficient and cost-effective way to resolve their dispute. After all, the mediator already knows the facts of the case. Why waste time and money getting another neutral up to speed? This design choice, however, may collide with the mediator ethical mandates of party self-determination, neutral impartiality, confidentiality, …

Copyright’S Capacity Gap, Andrew Gilden, Eva E. Subotnik

Faculty Publications

Most areas of law require that individuals meet a certain threshold of capacity before their decisions — e.g., to marry, to enter into a contract, or to execute an estate plan — are given legal effect. Copyright law, by contrast, gives legal effect to creative decisions by granting the decisionmaker many decades of exclusive rights so long as they are a human being and have demonstrated a “creative spark.” This Article examines the overlooked consequences of this gap in capacity standards between copyright and other areas of law. It shows that this gap has produced numerous opportunities for vulnerable creators …

Humanizing Virtual Dispute Resolution, Elayne E. Greenberg

Faculty Publications

(Excerpt)

How might neutrals and advocates foster interpersonal dynamics when conducting arbitrations and mediations virtually, consistent with the ethical obligations of each profession and the ethical underpinnings of each process?

Virtual dispute resolution for commercial dispute resolution has become the new normal. Yet, the dispute resolution listserves are still peppered with posts from mediators and arbitrators who, although publicly extolling their own commitments to their impartiality and neutrality, are also simultaneously voicing their strong preferences for conducting their dispute resolution processes in person. According to these neutrals, they are unable to attain the same results when the process is conducted …

Investing In Abolition, Sandeep Singh Dhaliwal

Faculty Publications

This Article situates the prison within a broader macro-financial trend, what I call “community capture.” As private equity firms have consolidated the market for carceral services, they have also gained control over other essential social infrastructure, like housing and healthcare. By layering debt, fees, and aggressive profit expectations over companies or assets that provide for basic needs, their strategies intensify the economic pressures communities face. These strategies rely on various forms of coordination and legal protection from contestation. To demonstrate this process in the context of mass incarceration, this Article tells the story of Securus, a dominant carceral service company …

A Ph-Responsive Shell-Sheddable Polymeric Nanoparticles As The Photodynamic Drug Carrier: Synthesis, Characterization, And In Vitro Studies, Jing Lu

Turkish Journal of Biology

Shell-sheddable nanoparticles, composed of amphiphilic blockpolymers, have emerged as an attractive vehicle for the site-specific delivery of therapeutic agents. In this study, pH-responsive sheddable copolymers bearing an orthoester linker were synthesized via the ring-opening polymerization between γ-benzyl-L-glutamate N-carboxyanhydride and orthoester-bearing poly (ethylene glycol) macroinitiator (PEG-pH-NH₂). The obtained poly (ethylene glycol)-b-poly(γ-benzyl-L-glutamate) (PEG-PBLG) could form stable nanoparticles in aqueous solutions due to the amphiphilic nature of the block copolymers. The PEG-PBLG-based nanoparticle exhibited good stability in physiological conditions (pH 7.4), whereas the nanoparticle was disassembled under acidic conditions (pH 5.0). The nanoparticles could encapsulate a photosensitizer, protophorphyrin IX …

Application Of Scanning Acoustic Microscopy For Evaluation Of Mmp Activation In Multiple Cancer Cell Lines With A Smart Probe, Hasan Ozan Otaş, Nasi̇re Uluç, İrem Demi̇rkan, Ayli̇n Alkan, Açelya Yilmazer, Seda Yaşa Ercan, Davod Khalafkhany, Nesri̇n Özören, Mehmet Burçi̇n Ünlü

Turkish Journal of Biology

Background/aim: Matrix metalloproteinases (MMPs) play an important role in the evaluation of many cancer types; however, the detection usually presents a challenge. Further assays for a better understanding of the fundamental roles of MMPs in pathophysiology are still needed. We aimed to use an activatable probe in scanning acoustic microscopy (SAM) to evaluate acoustically if the probe can aid the visualization of the effects of in vitro MMP activity. Materials and methods: We applied scanning acoustic impedance microscopy to obtain acoustic impedance maps of the cell line models of HT1080, THP-1, and SK-MEL-28 with and without MMPSense 680 probe incubation. …

Functional Enhancement Of Acute Infracted Heart By Coinjection Of Autologous Adipose-Derived Stem Cells With Matrigel, Baozhu Wang, Mengmeng Wang, Yan Li, Meihua Shao, Dan Zhan, Shiqi Yan, Xiang Ma, Yitong Ma

Turkish Journal of Biology

Recent clinical developments in tissue bioengineering have applications in acute cardiac ischemia and infarction and include the use of stem cells that combine injectable scaffold material. This study aimed to evaluate the effects of adipose-derived stem cells (ADSCs) that combine the Matrigel scaffold on cardiac morphology/functions. The autologous ADSCs myocardial infarction (MI) model was induced by the permanent ligation method of the left anterior descending coronary artery (LAD). MI-operated rats were randomly divided into PBS group, Matrigel group, PBS plus ADSCs group (PBS+ADSCs), and Matrigel plus ADSCs group (Matrigel+ADSCs). Matrigel was used as an injectable scaffold. Rats with a 1-week-old …

Tsrna-15797-Modified Bmsc-Derived Exosomes Mediate Lfng To Induce Angiogenesis In Osteonecrosis Of The Femoral Head, Shanhong Fang, Mengqiang You, Jie Wei, Peng Chen

Turkish Journal of Biology

Background: Osteonecrosis of the femoral head (ONFH) is an ischemic disease characterized by the impairment of angiogenesis. We have previously elucidated the role of tsRNAs and BMSC exosomes in ONFH, but whether tsRNA-modified BMSC exosomes promote angiogenesis in ONFH remains unclear. Methods: The expression of angiogenesis-related tsRNA in plasma exosomes from ONFH patients was examined by q-PCR. The function of tsRNA in HUVECs was identified by CCK-8 and angiogenesis assay. Exosomes purified from tsRNA-15797 overexpressed BMSCs were cocultured with HUVECs to examine their role in angiogenesis. The molecule mechanism of tsRNA-15797-modified exosomes was explored by RNA sequencing, dual-luciferase assay, and …

Methionine Restriction And Cancer Treatment: A Systems Biology Study Of Yeast To Investigate The Possible Key Players, Esra Börklü

Turkish Journal of Biology

Background/aim: Dietary restriction, mainly carbon and/or methionine restriction are among the upcoming supporting interventions along with chemotherapy in various cancers. Although dietary restriction has been proven to be beneficial, the main cellular machineries affected by its administration lacks deeper information considerably, a notable pitfall in its use as a personalized nutritional approach. Materials and methods: In this study, cellular effects of methionine restriction on a yeast model are explored via systems biology approaches. The methionine biosynthesis network, constructed by integrating interaction data with gene ontology terms, was analysed topologically, and proved to be informative about the intertwined relationship of methionine …