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13c Labeling For Cho Cell Metabolism Tracing And Ms Based Analysis For Advanced Upstream Culture Monitoring To Support Cqa Understanding, Xin Bush

Open Access Dissertations

Monoclonal antibodies (mAbs) make up a large part of biopharmaceutical sales due to their great success in treating cancer, autoimmune, and cardiovascular diseases. Due to an increasing number of mAbs using innovative approaches in drug development, combined with changes in the drug market, there is an increased need for optimizing process intensification and quality control. Thus, a profound understanding of cellular metabolism and how this impacts the protein product’s critical quality attributes (CQA) would be of great value. Among the quality attributes of mAbs, post-translational modifications such as N-linked glycosylation are of particular importance as they affect the activity, efficacy, …

Environmental Toxicity Of Isoxazoline Drugs On Non-Target Species, Haley M. Cooper

Theses and Dissertations

Lyme disease affects tens of thousands of people every year in the United States, causing serious illness and even death. It is caused by the bacterium, Borrelia burgdorferi, through infected black-legged tick bites. The range of these ticks is expanding, causing Lyme disease cases to increase. Considerable effort has been put toward research and eradication of Lyme disease, with limited success. Isoxazoline drugs, commonly used to prevent flea and tick infestations in dogs, could be dispatched in the field to keep black-legged ticks off the reservoir host of Lyme disease, the White-footed mouse. Little research has been done on …

The Perceptions Of People With Dementia, Care Partners, Cnas/Sitters, And Providers During The Covid-19 Pandemic Up To Now: A Multiple Case Study, Gaudensia Awuor

Nursing Theses and Dissertations

The perceptions of people with dementia, care partners, CNAs/sitters, and providers during the COVID-19 pandemic until now can be useful in formulating better ways to care for people with dementia in future disasters or pandemics. The study explored the perceptions of people with dementia, care partners, CNAs/sitters, and providers during the COVID-19 pandemic, from onset until now, to help formulate better ways to care for people with dementia during a pandemic or disaster. Algase’s Need-driven Dementia-compromised Behavior Model guided this comparative, multiple-case study. Six people with dementia, six care partners, five CNAs and one sitter, and six providers were interviewed …

Molecular Mechanisms Of Wild-Type And Mutant Prion Formation, Daniel J. Walsh

Dartmouth College Ph.D Dissertations

Prion diseases are a class of infectious neurodegenerative disorders which affect humans and many other mammalian species. The causative agent is a unique pathogen known as a prion or PrPSc, a misfolded form of a host-encoded glycoprotein which replicates by templated conformational change. Distinct strains of prions with unique phenotypic and pathologic presentations appear to be encoded by subtle conformational changes within the misfolded protein. While the general manner of prion transmission is known, our detailed understanding of these mechanisms remains incomplete, limiting efforts for the discovery or design of therapeutic treatments for these fatal diseases. In vitro synthesis of …

Assessing The Efficacy Of A Novel Galactooligosaccharide To Promote Fish Health And Robustness, Lucy May Sykes

School of Biological and Marine Sciences Theses

Aquaculture has been steadily increasing global production; however, there remains a progressively greater demand for sustainable sources of protein, whilst the prevalence of disease represents a major constraint to industry growth. Feed additives are gaining traction as useful tools to help combat disease and promote health, and previous research has documented benefits to the mucosal surfaces and microbial communities within a range of teleost species. Three experimental in vivo feeding trials were undertaken using a range of in-depth analyses, such as growth performance, haematology, immunology, intestinal histology, intestinal gene expression and intestinal bacterial 16S rRNA sequence libraries. Studies were conducted …

Nanomaterials For Cardiovascular Engineering, Roya Bagheri

Dissertations, Master's Theses and Master's Reports

Cardiovascular diseases and disorders (i.e., those related to heart and blood vessels) are the main reasons for mortality worldwide. Nanomaterials, with their unique morphologies and properties, have a great potential for advancing cardiovascular engineering to treat diseases and disorders. In this dissertation, several cardiovascular applications of conductive nanomaterials were investigated. First, a conductive nanomaterial was explored to fabricate biohybrid nanomaterial-cardiomyocyte (CM – heart muscle cell) systems. Using Carbon Nanotube (CNT) forest as a 3D porous and conductive scaffold was investigated. The influence of the CNT forest on the viability, attachment, and spreading of CMs and their genetic information was …

Sustainable Development Goals, Between Politics And Soft Law: The Emergence Of “Political Normativity” In International Law, Ilias Bantekas, Katerina Akestoridi

Emory International Law Review

International lawyers tend to draw sharp distinctions between law and politics. Yet, despite the Sustainable Development Goals (SDGs) and its predecessors being a product of politics, the commitments undertaken therefrom suggest a new brand of politics where functionality overrides the search for normative characteristics. The political dimension of the SDGs overshadows its legal dimension because the implementation of the key stakeholders’ political commitments is crucial in achieving the SDGs’ stated aims. As international law and international relations are moving towards universal common interests and away from strict international normativity, the SDGs can best be described as a hybrid between soft …

Impacts Of Introduced Earthworms On Sugar Maple Sap Chemistry And The Discourse Of Invasion Ecology, Shelby Nicole Lane-Clark

Dissertations, Master's Theses and Master's Reports

To delve into further understanding of the relationships between introduced earthworms, forest ecosystems, and the human systems they impact, two approaches were taken. In the first approach, we seek to explore ways we can shift the discourse within and regarding the field of invasion ecology by re-framing how we approach discussion, management, and education regarding introduced species. Language issues surrounding introduced species through the example of earthworms in North America are described and potential solutions are provided, including a repositioned perspective that may facilitate better relationships with the natural world. The goal is to shift the language to be more …

Machine Learning Methods For Prediction Of Human Infectious Virus And Imputation Of Hla Alleles, Xiaoqing Gao

Dissertations, Master's Theses and Master's Reports

This dissertation contains three Chapters. The following is a concise description of each Chapters.

In Chapter 1, we introduced the Random Forest, a machine learning method, to foresee whether a virus is capable of infecting humans. The Covid pandemic informs us the importance of predicting the ability of a zoonotic virus that can infect humans from its genomic sequence. We used the -mer with and as features of a virus to predict if it can affect humans. We further employed the Boruta algorithm to select the important features, then fed those important features into the Random Forest method to train …

Virus Inactivation By Novel Virucidal Materials, Sneha Singh

Dissertations, Master's Theses and Master's Reports

The development of virucidal coatings to inactivate a broad spectrum of viruses has gained popularity. Antiviral coating of personal protective equipment such as facial coverings can reduce the accumulation of viral load and mitigate transmission of airborne illnesses, which occur primarily through direct contact with respiratory aerosols or droplets carrying infectious viruses. Moreover, modification of PPE to inactivate viruses upon contact improves their reusability and reduces waste. Coating high frequency touch surfaces with similar virucidal coatings can reduce the risk of disease transmission associated with contaminated surfaces. This study explores the development of three novel virucidal materials to control the …

Biogeography Of Special Metabolites Produced By Ant-Associated Bacteria, Katherine Hansen

Scripps Senior Theses

There is an urgent and growing need for novel antibiotics with the growing prevalence of antibiotic-resistant pathogens. Ecologically-guided discovery is a strategy that focuses on how and where specially evolved metabolites such as antibiotics are used in nature. This strategy reveals unique active molecules with potential for human use. An unexplored niche with great potential for antibiotic discovery exists with Southwestern fungus-farming Trachymyrmex ants and their Actinobacteria symbionts. In exchange for nutrients from the ant, the ants’ bacterial symbiont produces antifungal molecules that protect the ants’ fungal cultivar from invasion by pathogenic microorganisms. They also produce antibacterial molecules to protect …

Show Us The Data: Global Covid-19 Wastewater Monitoring Efforts, Equity, And Gaps, Colleen C. Naughton, Fernando A. Roman, Ana Grace F. Alvarado, Arianna Q. Tariqi, Matthew A. Deeming, Krystin F. Kadonsky, Kyle Bibby, Aaron Bivins, Gertjan Medema, Warish Ahmed, Panagis Katsivelis, Vajra Allan, Ryan Sinclair, Joan B. Rose

Faculty Publications

A year since the declaration of the global coronavirus disease 2019 (COVID-19) pandemic, there were over 110 million cases and 2.5 million deaths. Learning from methods to track community spread of other viruses such as poliovirus, environmental virologists and those in the wastewater-based epidemiology (WBE) field quickly adapted their existing methods to detect SARS-CoV-2 RNA in wastewater. Unlike COVID-19 case and mortality data, there was not a global dashboard to track wastewater monitoring of SARS-CoV-2 RNA worldwide. This study provides a 1-year review of the “COVIDPoops19” global dashboard of universities, sites, and countries monitoring SARS-CoV-2 RNA in wastewater. Methods to …

Aircraft Wastewater Surveillance For Early Detection Of Sars-Cov-2 Variants — John F. Kennedy International Airport, New York City, August–September 2022, Robert C. Morfino, Stephen M. Bart, Andrew Franklin, Benjamin H. Rome, Andrew P. Rothstein, Thomas W.S. Aichele, Siyao Lisa Li, Aaron Bivins, Ezra T. Ernst, Cindy R. Friedman

Faculty Publications

No abstract provided.

The Human Factor: Managing Chaos And Change In Times Of Uncertainty, Stephanie Sterling Brasley, Adriana Popescu, Alicia Virtue, Delritta Hornbuckle

Library Scholarship

The unrelenting, unabating organizational, political, and human resource stressors library managers faced pushed the boundaries of their capacity to keep pace with the longer-term demands of this catastrophic time; however, their dedication and resilience, bolstered by wisdom from the models and theories discussed here, hold promise for a positive future.

When We're Coming From What Would An Hiv Doula Do? On Pandemic Time(S), Alexandra Juhasz, Theodore Kerr, Jennifer Brier, Olivia Polk, Alex Fiahlo, Pato Hebert, Salonee Bhaman

Publications and Research

“When We’re Coming From” is a conversation across time and place among a group of writers and artists who are members of the What Would an HIV Doula Do? Collective. Seven of us have come together to have an asynchronous conversation about how our decades-long work to respond to the HIV/AIDS pandemic shapes our experience of the COVID-19 pandemic. Here we share six individual assessments of pandemic time to suggest ways that pandemics(s) shape our sense of time(s). What follows are our meditations on how and when we see ourselves, and the transitions of HIV/AIDS and COVID-19: “AIDS out of …

Elafibranor Modulates Ileal Macrophage Polarization To Restore Intestinal Integrity In Nash: Potential Crosstalk Between Ileal Il-10/Stat3 And Hepatic Tlr4/Nf-Κb Axes, Andrew Hakeem, Mohamed M. Kamal, Rasha A. Radwan, Basma A. Abdelrahman, Olfat A. Hammam, Mohey M. Elmazar, Aiman S. El-Khatib, Yasmeen M. Attia

Pharmacy

Experimental and clinical evidence implicate disrupted gut barrier integrity in provoking innate immune responses, specifically macrophages, towards the progression of non-alcoholic steatohepatitis (NASH). Peroxisome proliferator-activated receptors (PPARs), a subset of the nuclear receptor superfamily, act to fine-tune several metabolic and inflammatory processes implicated in NASH. As such, the current study was carried out to decipher the potential role of dual PPAR α/δ activation using elafibranor (ELA) on ileal macrophage polarization (MP) and its likely impact on the liver in a NASH setting. To achieve this aim, an in vitro NASH model using fat-laden HepG2 cells was first used to validate …

One Beat At A Time: Mitigating The Risk Factors Of Rheumatic Heart Disease Through Public Policies, Duy Phuong Huynh Nguyen

Theses

This thesis examines the challenges associated with acute rheumatic fever (ARF) and rheumatic heart disease (RHD) and the persistent prevalence of these diseases in the Australian context. In particular, the study focuses on the high resurgence of these preventable conditions within the Australian Aboriginal and Torres Strait Islander and Torres Strait Island population. The study begins with an initial analysis on the initial infection of the Group A streptococcus (GAS: Streptococcus pyogenes). We then examine current knowledge of the mechanisms which permit the transmission and development GAS infections, leading to ARF and RHD. This chapter explores current challenges associated with …

Inspire : Vol 6, Issue 1, Department Of Medicine

INSPIRE – Department of Medicine Archives

• Message from the Chair
• Medicine at National and International Forums
• Medicine at National and International Forums
• Annual Appreciation Event 2022
• Dr Bloomfield’s AKU visit
• Neurology | 22nd Annual Update Conference
• PADCON | 41st Conference
• Neurology Annual Appreciation Evening
• Nephrology | Mock TOACS Exam
• Featured Faculty: Dr. Zarmeen Nasim | Senior Instructor | Data Science Team “...Be honest with your work. Whatever you are doing in life, be loyal and committed…”
• Publications

Humans In The Loop, Rebecca Crootof, Margot E. Kaminski, W. Nicholson Price Ii

Law Faculty Publications

From lethal drones to cancer diagnostics, humans are increasingly working with complex and artificially intelligent algorithms to make decisions which affect human lives, raising questions about how best to regulate these “human-in-the-loop” systems. We make four contributions to the discourse.

First, contrary to the popular narrative, law is already profoundly and often problematically involved in governing human-in-the-loop systems: it regularly affects whether humans are retained in or removed from the loop. Second, we identify “the MABA-MABA trap,” which occurs when policymakers attempt to address concerns about algorithmic incapacities by inserting a human into a decisionmaking process. Regardless of whether the …

Duped By Dope: The Sackler Family’S Attempt To Escape Opioid Liability And The Need To Close The Non-Debtor Release Loophole, Bryson T. Strachan

Law Student Publications

The opioid epidemic continues to rage on in the United States, ravaging its rural populations. One of its main causes? OxyContin. Purdue Pharma (“Purdue”), the maker of OxyContin, aggressively marketed opioids to the American public while racking up a fortune of over $13 billion dollars for its owners,3 the Sackler family. As a result, roughly 3,000 lawsuits were filed against Purdue and members of the Sackler family. Generally, the lawsuits alleged that Purdue and members of the Sackler family knew OxyContin was highly addictive yet aggressively marketed high dosages of the drug and misrepresented the drug as nonaddictive and without …

Regulatory Elements Of Polyphosphate Biosynthesis, Marvin Qortez Bowlin

All ETDs from UAB

Polyphosphate (polyP) is an ancient, conserved, inorganic biomolecule. Biological systems have adapted many functions for this high-energy molecule, ranging from immune regulation in mammals to gene regulation in prokaryotes. Of particular interest is its use in bacterial stress responses. Bacteria use polyP to resist hazardous environmental elements like toxic molecules or nutrient starvation. In many bacterial species, polyP is synthesized by polyphosphate kinases (PPKs). PPK – discovered in Escherica coli (E. coli) – hydrolyzes adenosine-5’-triphosphate (ATP) to synthesize polyP. Species that use PPK develop multiple pathogenic defects when PPK activity is impaired, including, among others, antibiotic susceptibility. There is no …

Factors Influencing Physical Activity Among Us Adults With Chronic Health Conditions: Mediation And Moderation Analysis, Sami M. Alqahtani

All ETDs from UAB

The prevalence of chronic health conditions (CHCs) is rising across all age groups, genders, and racial/ethnic groupings, making them the main cause of death globally. The majority of CHCs deaths occur in middle-to-low-income countries, although they are also a significant health issue in developed countries. A higher risk of developing CHCs is linked to physical inactivity. Global societies are experiencing increased prevalence of CHCs, which are directly assoicated with rising healthcare costs, workforce attendance, productivity challenges, and academic success. However, increased exercise and physical activity (PA) are linked to lower odds of developing CHCs. In fact, regular PA can help …

Antimicrobial Activity Of Chitosan Nanoparticles Loaded With 0.7% Tetracycline Against Porphyromonas Gingivalis, Martina Amalia, Irma Ervina, Silvia, Erdi E. Nasution

Journal of Dentomaxillofacial Science

No abstract provided.

Employees’ Appraisal Of Workplace Changes During Covid-19 : Findings From A State-Owned Oil And Gas Company, Chaiwat Riratanaphong, Nuttawan Narmwiset

NAKHARA (Journal of Environmental Design and Planning)

This study aims to explore employees’ perceptions of workplace management practices (i.e. planning before workplace change, implementation process, management after workplace change) at the PTT headquarters building in Thailand. Another purpose is to examine workplace change development during COVID-19 to understand the organisation’s planning and processes, which serves as a means to contribute to Facilities Management and Corporate Real Estate Management (CREM) theory. Employees' responses to workplace management practices at different stages (i.e., before, during, and after workplace change) were affected by the company's strategic direction and management approach, particularly in terms of planning and implementing workplace change development. This …

Transforming Adversity Into Opportunity : Assessing User Satisfaction In Hospital Transformation In Lieu Of A Pandemic Through The Multi-Corridor Expansion Model For Epidemic Management And Environmental Design Enhancement, Omer Shujat Bhatti, Muhammad Asad Ghufran, Atta Ullah Shah

NAKHARA (Journal of Environmental Design and Planning)

The COVID-19 pandemic brought the world to a standstill, demonstrating the critical importance of preparedness and adaptation in the face of pandemics and epidemics. Pandemics and epidemics have been a persistent threat throughout human history, causing significant mortality and morbidity, as well as disrupting economies, societies, and politics. The COVID-19 pandemic, caused by the SARS-CoV-2 virus, had a profound impact on healthcare systems worldwide, leading to hospitals transforming into pandemic centers. This case study, conducted at Riphah International Hospital (RIH) in Islamabad, Pakistan, explores the transformation of an existing facility into a pandemic center, highlighting the challenges faced in managing …

Can Testing The Environment For Severe Acute Respiratory Coronavirus Virus 2 (Sars-Cov-2) Be A Signal For Coronavirus Disease 2019 (Covid-19) Cases Among Nursing Home Staff?, Gabrielle M. Gussin, Raveena D. Singh, Izabela Coimbra Ibraim, Raheeb Saavedra, Thomas T. Tjoa, Micaila Curtis, Kristine P. Nguyen, Ilhem Messaoudi, Susan S. Huang

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Prior to widespread COVID-19 vaccination, nursing homes were required to perform comprehensive weekly testing of staff. After the vaccine became available, testing became symptom-based for vaccinated staff despite continued waves of different severe acute respiratory coronavirus virus 2 (SARS-CoV-2) variants. Nursing homes need pragmatic, affordable strategies to identify SARS-CoV-2–positive staff because financial and job-related pressures drive reluctance to disclose mild symptoms. We sought to determine whether positive environmental samples for SARS-CoV-2 could serve as trigger for comprehensive staff testing in lieu of weekly testing of all staff.

Correlates Of Sars-Cov-2 Anti-Rbd Igg Antibody Titers Among Persons Experiencing Homelessness In Los Angeles, Adeline Nyamathi, Sanghyuk S. Shin, Brianna M. Doratt, Alexandria Jones-Patten, Benissa Salem, Lillian Gelberg, Darlene Lee, Dana Garfin, Kartik Yadav, Alicia H. Chang, Kathryn White, Nicholas Arce, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Objectives: People experiencing homelessness (PEH) have been especially impacted by the COVID-19 pandemic, likely due to increased vulnerabilities stemming from chronic diseases, substance use, and mental health conditions.

Design: A case–control study to assess the presence of antibodies against SARS-CoV-2 among PEH and associations with key variables.

Sample: A convenience sample of 97 PEH in Skid Row, Los Angeles.

Measurements: A structured questionnaire assessing socio-demographic, mental health, drug and alcohol use, health care access, pandemic stress, and other COVID-19-specific questions.

Results: We found high anti-receptor binding domain (RBD) IgG titers among five of 15 PEH who reported no prior COVID-19 …

Maternal Obesity Blunts Antimicrobial Responses In Fetal Monocytes, Suhas Sureshchandra, Brianna M. Doratt, Norma Mendza, Oleg Varlamov, Monica Rincon, Nicole E. Marshall, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Maternal pre-pregnancy (pregravid) obesity is associated with adverse outcomes for both mother and offspring. Amongst the complications for the offspring is increased susceptibility and severity of neonatal infections necessitating admission to the intensive care unit, notably bacterial sepsis and enterocolitis. Previous studies have reported aberrant responses to LPS and polyclonal stimulation by umbilical cord blood monocytes that were mediated by alterations in the epigenome. In this study, we show that pregravid obesity dysregulates umbilical cord blood monocyte responses to bacterial and viral pathogens. Specifically, interferon-stimulated gene expression and inflammatory responses to respiratory syncytial virus (RSV) and E. coli were significantly …

Sars-Cov-2 Infection And Seroconversion Rates In Healthcare Providers Prior To Covid-19 Vaccine Rollout, Sanghyuk S. Shin, Miriam Bender, Delphine C. Malherbe, Hannah Vasquez, Brianna M. Doratt, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Objective: A 6-month longitudinal surveillance study of asymptomatic healthcare providers (HCP) was carried out at a large urban academic medical center in the United States to assess whether their job occupation with higher exposure risks to SARS-CoV-2 would equate with higher risk of contracting COVID-19 at the beginning of the pandemic before COVID-19 vaccines were available.

Methods: A longitudinal cohort study design was used to collect and analyze immunological and virological monitoring data and self-report survey assessments of personal protective equipment (PPE) availability, adherence to infection control guidelines, and time spent on COVID-19 wards.

Results: Among 289 eligible participants, SARS-CoV-2 …

Single-Dose Vsv-Based Vaccine Protects Cynomolgus Macaques From Disease After Taï Forest Virus Infection, Paige Fletcher, Kyle L. O'Donnell, Brianna M. Doratt, Delphine C. Malherbe, Chad S. Clancy, Joseph F. Rhoderick, Friederike Feldmann, Patrick W. Hanley, Thomas G. Ksiazek, Thomas W. Geisbert, Ilhem Messaoudi, Andrea Marzi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Taï Forest virus (TAFV) is a lesser-known ebolavirus that causes lethal infections in chimpanzees and is responsible for a single human case. Limited research has been done on this human pathogen; however, with the recent emergence of filoviruses in West Africa, further investigation and countermeasure development against this virus is warranted. We developed a vesicular stomatitis virus (VSV)-based vaccine expressing the TAFV glycoprotein as the viral antigen and assessed it for protective efficacy in nonhuman primates (NHPs). Following a single high-dose vaccination, NHPs developed antigen-specific binding and neutralizing antibodies as well as modest T cell responses. Importantly, all vaccinated NHPs …