Digital Commons Network^™

Aberrant T-Cell Exhaustion In Severe Combined Immunodeficiency Survivors With Poor T-Cell Reconstitution After Transplantation, Roxane Labrosse, Shalini Shenoy, Et Al.

2020-Current year OA Pubs

BACKGROUND: Severe combined immunodeficiency (SCID) comprises rare inherited disorders of immunity that require definitive treatment through hematopoietic cell transplantation (HCT) or gene therapy for survival. Despite successes of allogeneic HCT, many SCID patients experience incomplete immune reconstitution, persistent T-cell lymphopenia, and poor long-term outcomes.

OBJECTIVE: We hypothesized that CD4

METHODS: We analyzed markers of exhaustion in blood samples from 61 SCID patients at a median of 10.4 years after HCT.

RESULTS: Compared to post-HCT SCID patients with normal CD4

CONCLUSIONS: Recipients of unconditioned HCT for SCID may develop late post-HCT T-cell exhaustion as a result of diminished production of T-lineage …

Amplitudes Of Resting-State Functional Networks - Investigation Into Their Correlates And Biophysical Properties, Soojin Lee, Janine D Bijsterbosch, Fidel Alfaro Almagro, Lloyd Elliott, Paul Mccarthy, Bernd Taschler, Roser Sala-Llonch, Christian F Beckmann, Eugene P Duff, Stephen M Smith, Gwenaëlle Douaud

2020-Current year OA Pubs

Resting-state fMRI studies have shown that multiple functional networks, which consist of distributed brain regions that share synchronised spontaneous activity, co-exist in the brain. As these resting-state networks (RSNs) have been thought to reflect the brain's intrinsic functional organization, intersubject variability in the networks' spontaneous fluctuations may be associated with individuals' clinical, physiological, cognitive, and genetic traits. Here, we investigated resting-state fMRI data along with extensive clinical, lifestyle, and genetic data collected from 37,842 UK Biobank participants, with the object of elucidating intersubject variability in the fluctuation amplitudes of RSNs. Functional properties of the RSN amplitudes were first examined by …

Pregnancy-Induced Differential Expression Of Sars-Cov-2 And Influenza A Viral Entry Factors In The Lower Respiratory Tract, Tusar Giri, Santosh Panda, Arvind Palanisamy

2020-Current year OA Pubs

Despite differences in the clinical presentation of coronavirus disease-19 and pandemic influenza in pregnancy, fundamental mechanistic insights are currently lacking because of the difficulty in recruiting critically ill pregnant subjects for research studies. Therefore, to better understand host-pathogen interaction during pregnancy, we performed a series of foundational experiments in pregnant rats at term gestation to assess the expression of host entry factors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A virus (IAV) and genes associated with innate immune response in the lower respiratory tract. We report that pregnancy is characterized by a decrease in host factors mediating …

Expression Of Non-Phosphorylatable S5a-L-Plastin Exerts Phenotypes Distinct From L-Plastin Deficiency During Podosome Formation And Phagocytosis, Xue Lin, Praveen Krishnamoorthy, Emma C Walker, Hemant Joshi, Sharon Celeste Morley

2020-Current year OA Pubs

No abstract provided.

The Relationship Of Trait-Like Compassion With Epigenetic Aging: The Population-Based Prospective Young Finns Study, Henrik Dobewall, Liisa Keltikangas-Järvinen, Saara Marttila, Pashupati P Mishra, Aino Saarinen, C Robert Cloninger, Igor Zwir, Mika Kähönen, Mikko Hurme, Olli Raitakari, Terho Lehtimäki, Mirka Hintsanen

2020-Current year OA Pubs

INTRODUCTION: Helping others within and beyond the family has been related to living a healthy and long life. Compassion is a prosocial personality trait characterized by concern for another person who is suffering and the motivation to help. The current study examines whether epigenetic aging is a potential biological mechanism that explains the link between prosociality and longevity.

METHODS: We used data from the Young Finns Study that follows six birth-cohorts from age 3-18 to 19-49. Trait-like compassion for others was measured with the Temperament and Character Inventory in the years 1997 and 2001. Epigenetic age acceleration and telomere length …

Treg-Specific Deletion Of The Phosphatase Shp-1 Impairs Control Of Inflammation In Vivo, Qinlei Gu, Kenneth S Tung, Ulrike M Lorenz

2020-Current year OA Pubs

INTRODUCTION: To achieve a healthy and functional immune system, a delicate balance exists between the activation of conventional T cells (Tcon cells) and the suppression by regulatory T cells (Treg). The tyrosine phosphatase SHP-1, a negative regulator of TCR signaling, shapes this 'activation-suppression' balance by modulating Tcon cell resistance to Treg-mediated suppression. Treg cells also express SHP-1, but its role in influencing Treg function is still not fully understood.

METHODS: We generated a Treg-specific SHP-1 deletion model,

RESULTS: We show that SHP-1 modulates Treg suppressive function at different levels. First, at the intracellular signaling level in Treg cells, SHP-1 attenuates …

The In Vivo Study Of Cardiac Mechano-Electric And Mechano-Mechanical Coupling During Heart Development In Zebrafish, Jonathan S Baillie, Alex Gendernalik, Deborah M Garrity, David Bark Jr, T Alexander Quinn

2020-Current year OA Pubs

In the adult heart, acute adaptation of electrical and mechanical activity to changes in mechanical load occurs

Phagocytosis Is A Primary Determinant Of Pulmonary Clearance Of Clinical Klebsiella Pneumoniae Isolates, Rick Van Der Geest, Hongye Fan, Hernán F Peñaloza, William G Bain, Zeyu Xiong, Naina Kohli, Emily Larson, Mara L G Sullivan, Jonathan M Franks, Donna B Stolz, Ryota Ito, Kong Chen, Yohei Doi, Melanie J Harriff, Janet S Lee

2020-Current year OA Pubs

INTRODUCTION:

METHODS: We screened 19 clinical respiratory

RESULTS: The respiratory

CONCLUSION: Altogether, these findings show that phagocytosis is a primary determinant of pulmonary clearance of clinical

The Cerebrospinal Fluid Immune Cell Landscape In Animal Models Of Multiple Sclerosis, Gregory F Wu

2020-Current year OA Pubs

The fluid compartment surrounding the central nervous system (CNS) is a unique source of immune cells capable of reflecting the pathophysiology of neurologic diseases. While human clinical and experimental studies often employ cerebrospinal fluid (CSF) analysis, assessment of CSF in animal models of disease are wholly uncommon, particularly in examining the cellular component. Barriers to routine assessment of CSF in animal models of multiple sclerosis (MS) include limited sample volume, blood contamination, and lack of feasible longitudinal approaches. The few studies characterizing CSF immune cells in animal models of MS are largely outdated, but recent work employing transcriptomics have been …

Targeted Aso-Mediated Atp1a2 Knockdown In Astrocytes Reduces Sod1 Aggregation And Accelerates Disease Onset In Mutant Sod1 Mice, Abhirami K. Iyer, Kathleen M. Schoch, Anthony Verbeck, Grant Galasso, Hao Chen, Sarah Smith, Anna Oldenborg, Timothy M. Miller, Celeste M. Karch, Azad Bonni

2020-Current year OA Pubs

Astrocyte-specific ion pump α2-Na+/K+-ATPase plays a critical role in the pathogenesis of amyotrophic lateral sclerosis (ALS). Here, we test the effect of Atp1a2 mRNA-specific antisense oligonucleotides (ASOs) to induce α2-Na+/K+-ATPase knockdown in the widely used ALS animal model, SOD1*G93A mice. Two ASOs led to efficient Atp1a2 knockdown and significantly reduced SOD1 aggregation in vivo. Although Atp1a2 ASO-treated mice displayed no off-target or systemic toxicity, the ASO-treated mice exhibited an accelerated disease onset and shorter lifespan than control mice. Transcriptomics studies reveal downregulation of genes involved in oxidative response, metabolic pathways, trans-synaptic signaling, and upregulation of genes involved in glutamate receptor …

A Review Of Technological Innovations Leading To Modern Endovascular Brain Aneurysm Treatment, David C Lauzier, Anna L Huguenard, Anja I Srienc, Samuel J Cler, Joshua W Osbun, Arindam R Chatterjee, Ananth K Vellimana, Akash P Kansagra, Colin P Derdeyn, Dewitte T Cross, Christopher J Moran

2020-Current year OA Pubs

Tools and techniques utilized in endovascular brain aneurysm treatment have undergone rapid evolution in recent decades. These technique and device-level innovations have allowed for treatment of highly complex intracranial aneurysms and improved patient outcomes. We review the major innovations within neurointervention that have led to the current state of brain aneurysm treatment.

Rev-Erb Activation As A Novel Pharmacological Approach For Treating Inflammatory Pain, Sangeet Makhija, Joshua D Griffett, Giri Babu Veerakanellore, Thomas P Burris, Bahaa Elgendy, Kristine Griffett

2020-Current year OA Pubs

Pain is a complex problem affecting millions of people worldwide. The current therapies to reduce pain are limited as many treatment options inadequately address the causes of pain, lead to tolerance of the drug, or have adverse effects including abuse potential. While there are many causes of pain, one underlying mechanism to the pathogenesis and maintenance of pain conditions is chronic inflammation driven by the NLRP3 inflammasome. Several inflammasome inhibitors are currently under investigation however have the potential to suppress the functioning of the innate immune system, which may cause unwanted affects in patients. Here, we show that the nuclear …

Disease-Causing Mutations In Genes Encoding Transcription Factors Critical For Photoreceptor Development, Chi Sun, Shiming Chen

2020-Current year OA Pubs

Photoreceptor development of the vertebrate visual system is controlled by a complex transcription regulatory network. OTX2 is expressed in the mitotic retinal progenitor cells (RPCs) and controls photoreceptor genesis. CRX that is activated by OTX2 is expressed in photoreceptor precursors after cell cycle exit. NEUROD1 is also present in photoreceptor precursors that are ready to specify into rod and cone photoreceptor subtypes. NRL is required for the rod fate and regulates downstream rod-specific genes including the orphan nuclear receptor NR2E3 which further activates rod-specific genes and simultaneously represses cone-specific genes. Cone subtype specification is also regulated by the interplay of …

Precision Neuromodulation: Promises And Challenges Of Spinal Stimulation For Multi-Modal Rehabilitation, Maria F Bandres, Jefferson L Gomes, Gerson N Moreno Romero, Avery R Twyman, Jacob Graves Mcpherson

2020-Current year OA Pubs

Spinal cord injury results in multiple, simultaneous sensorimotor deficits. These include, but are not limited to, full or partial paralysis of muscles below the lesion, muscle spasms, spasticity, and neuropathic pain. Bowel, bladder, and sexual dysfunction are also prevalent. Yet, the majority of emerging spinal stimulation-based therapies focus on a single issue: locomotor rehabilitation. Despite the enormous potential of these translational advances to transform the lives of people living with spinal cord injury, meaningful recovery in other domains deemed critical priorities remains lacking. Here, we highlight the importance of considering the diverse patterns of neural transmission that underlie clinically similar …

A Novel Approach For Assessing Bias During Team-Based Clinical Decision-Making, Natalie Pool, Megan Hebdon, Esther De Groot, Ryan Yee, Kathryn Herrera-Theut, Erika Yee, Larry A Allen, Ayesha Hasan, Joann Lindenfeld, Elizabeth Calhoun, Molly Carnes, Nancy K Sweitzer, Khadijah Breathett

2020-Current year OA Pubs

Many clinical processes include multidisciplinary group decision-making, yet few methods exist to evaluate the presence of implicit bias during this collective process. Implicit bias negatively impacts the equitable delivery of evidence-based interventions and ultimately patient outcomes. Since implicit bias can be difficult to assess, novel approaches are required to detect and analyze this elusive phenomenon. In this paper, we describe how the de Groot Critically Reflective Diagnoses Protocol (DCRDP) can be used as a data analysis tool to evaluate group dynamics as an essential foundation for exploring how interactions can bias collective clinical decision-making. The DCRDP includes 6 distinct criteria: …

Iron Homeostasis And Post-Hemorrhagic Hydrocephalus: A Review, Shelei Pan, Andrew T Hale, Mackenzie E Lemieux, Dhvanii K Raval, Thomas P Garton, Brooke Sadler, Kelly B Mahaney, Jennifer M Strahle

2020-Current year OA Pubs

Iron physiology is regulated by a complex interplay of extracellular transport systems, coordinated transcriptional responses, and iron efflux mechanisms. Dysregulation of iron metabolism can result in defects in myelination, neurotransmitter synthesis, and neuronal maturation. In neonates, germinal matrix-intraventricular hemorrhage (GMH-IVH) causes iron overload as a result of blood breakdown in the ventricles and brain parenchyma which can lead to post-hemorrhagic hydrocephalus (PHH). However, the precise mechanisms by which GMH-IVH results in PHH remain elusive. Understanding the molecular determinants of iron homeostasis in the developing brain may lead to improved therapies. This manuscript reviews the various roles iron has in brain …

Glaucoma And Microglia-Induced Neuroinflammation, Makoto Ishikawa, Yukitoshi Izumi, Kota Sato, Taimu Sato, Charles F Zorumski, Hiroshi Kunikata, Toru Nakazawa

2020-Current year OA Pubs

Glaucoma is a multifactorial neurodegenerative disease characterized by a progressive optic neuropathy resulting in visual field defects. Elevated intraocular pressure (IOP) is the greatest risk factor for the development of glaucoma, and IOP reduction therapy is the only treatment currently available. However, there are many cases in which retinal degeneration progresses despite sufficient control of IOP. Therefore, it is important to elucidate the pathophysiology of glaucoma that is resistant to current IOP lowering therapies. Experiments using animal glaucoma models show the relationships between microglial neuroinflammatory responses and damage of retinal ganglion cells (RGCs). Inhibition of neuroinflammatory pathways associated with microglial …

Arts 3153 Perception To Abstraction, Marc Mitchell

All Syllabi

No abstract provided.

Arts 3403 Printmaking Introduction, Acadia Kandora

All Syllabi

No abstract provided.

Arts 3523 Ceramics: Wheelthrowing I, Qwist Joseph

All Syllabi

No abstract provided.

Community Drawing And Storytelling To Understand The Place Experience Of Walking And Cycling In Dushanbe, Tajikistan, Carl A. Smith

Landscape Architecture Faculty Publications and Presentations

Transit infrastructure is a critical determinant of the layout and sustainability performance of residential landscapes and neighborhoods. Though the spatial aspects of transit design and their associated impact on health, congestion, air pollution, accident rates, and emissions of greenhouse gases are well understood, the experiential-qualitative aspects of mobility have often been ignored in the travel and transport literature. This paper presents the place-understandings of pedestrians and cyclists concerning neighborhood safety in Dushanbe, the capital city of Tajikistan. Community perspectives were captured through drawing and storytelling workshops as a method of public engagement through creative experience. While reporting on the veracity …

Privacy In Modern American Law And Society, Joanna L. Grossman, Lawrence M. Friedman

Faculty Journal Articles and Book Chapters

No abstract provided.

Waiting Too Long To Move At Green Lights, Michael Landreth

Open Access Dissertations

A literary critique of the American working-class experience must be accessible to working-class readers if it hopes to impact or perform any service to its intended audience. Due to its ability to interrogate forms of institutional control that maintain the social hierarchy under which working-class Americans live, a formally experimental literary approach proves likely to be the best approach for critiquing the American working-class experience. Unfortunately, because formally experimental literature requires access to the time and resources needed for training in and exposure to a tradition of artistic experimentation, most working-class readers find formally experimental literature inaccessible. The work in …

Home Health Care Nurses’ Experiences And Perceptions Of Providing Care To Older Adults During The Covid-19 Pandemic: A Qualitative Descriptive Study, Joan R. Dugas

Open Access Dissertations

Home health care nurses were essential front line healthcare providers during the COVID-19 pandemic in the United States. Research in this area has primarily focused on the experiences of nurses in the acute and long-term care settings. This study aims to: (1) gain an understanding of the subjective experiences and perceptions of home health care nurses providing care to OAs during the COVID-19 pandemic and (2) elucidate aspects affecting the care provided to OAs during the COVID-19 pandemic.

Twenty nurses were interviewed between July 2022 and September 2022. The interviews were semi-structured and were analyzed using reflexive thematic analysis. Four …

Flux To Flow: A Clearer View Of Earth’S Water Cycle Via Neural Networks And Satellite Data, Albert Eric Larson

Open Access Dissertations

Earth’s weather and climate changes dynamically, and humans must monitor the evolution of our environment or fail to plan and adapt. Water is our most valuable resource, and as such it is of the utmost importance to have a continuous view of its movements as water cycles over, in, and under land, in the atmosphere, and in the sea. One way that we observe the water cycle is with satellite measurements. Unfortunately, the resolution of these instruments is typically too coarse to visualize many transient phenomena of interest. If their vision is fine enough, the instrument captures only a small …

The Effect Of Acceptance And Commitment Therapy On Value Consistency During A Weight Loss Program, Leah Dorfman

Open Access Dissertations

In the last decade, the prevalence of obesity has increased significantly in populations worldwide. It is well established that overweight and obesity are associated with a host of deleterious health consequences. Standard Behavioral Therapy (SBT) for weight loss has been demonstrated to yield only modest outcomes with poor long-term maintenance. Therefore, efforts must be made to improve upon what is currently considered to be best practices in weight loss interventions, especially with a goal of improving retention and long-term maintenance. Acceptance and Commitment Therapy (ACT) is a behavioral and cognitive intervention that implements both acceptance and mindfulness processes, and commitment …

Microfluidic Paper-Based Devices For The Detection Of Chemical And Biological Agents, Hojat Heidaribafroui

Open Access Dissertations

This work was motivated by two needs: to develop low-cost, high-sensitivity, and accurate systems for detecting phosphate in seawater, and to create a platform technology by integrating Bi-Material Cantilever (B-MaC) valves for rapid and affordable biosensors that can be used at home or point-of-care settings to detect pathogens.

Detecting low levels of phosphate in the seawater using an inexpensive sensor is crucial for marine scientists to make informed decisions on where to measure next, compared to the current method of collecting samples and analyzing them ashore. To address these needs, we developed a portable infrared lightbox that can be wirelessly …

Queer And Trans Inclusion In Finnish Teacher Education And Educational Research, Megan C. Jones

Open Access Dissertations

This dissertation presents findings from a case study on queer and trans inclusion in a Finnish graduate program in educational research. The study was also able to expand to include experiences and perspectives from teacher education programs at the same university. This research serves an incredibly timely purpose of bringing issues of queer and trans inclusion to light in a context that is marketed as having idealistic educational practices and programs. Overall findings indicate that even in Finland, a country often looked at as an exemplary model of educational reform, there is a lack of queer and trans inclusion in …

A Case Study Of Noncredit To Credit Pathways At Community Colleges, Amy Grzybowski

Open Access Dissertations

A high school diploma is no longer the path to financial stability; a person needs at least a postsecondary degree or credential to earn a median income (Carnevale, et al. 2019). Community colleges need to work to create opportunities for all students, including those that may enter school as an adult, and not straight from high school, to attain a certificate or degree. This study examines two public community colleges in the United States that have both noncredit and credit programs that are in different stages of advancement of clear, articulable pathways. The purpose of this qualitative case study was …

“If You Are Trying To Help Students, You Should Probably Ask Them How You Can Help”: Testimonios Of Latina/O/ Hispanic College Students Who Persisted To Graduation, Joise Garzon

Open Access Dissertations

Latinx student enrollment in higher education institutions has been increasing and will continue to rise (McFarland et al., 2018), yet Latinx students have lower degree completion rates than their peers from other racial and ethnic backgrounds (National Center for Education Statistics, 2019). Higher education institutions need to learn directly from the lived experiences of this diverse population of students to better support their college completion efforts (Arbelo-Marrero & Milacci, 2018; Flink, 2018). The purpose of this qualitative study was to explore the experiences of Latinx students who persisted to graduation at a four-year Hispanic Serving Institution by examining two research …