Digital Commons Network^™

Culturally Responsive Education: Creating A Safe And Inclusive Environment For Lgbtq+ Students, Kassidi M. Adams

Masters Projects

This paper explores research that discusses the impact of a culturally responsive education and lack-of for LGBTQ+ students and recommends a solution to ensure all students are safe and represented in schools. The research cited varies in their definitions and uses of the term LGBTQ+. This paper will use the term LGBTQ+, an abbreviation for lesbian, gay, bisexual, transgender, queer, and more communities; however, the abbreviation used in the referenced research may be shortened to LG, LGB, LGBT, or LGBTQ to reflect only specific communities included in LGBTTTQQIAAP (VandenBos, 2015). This paper will also focus on inclusion, which refers to …

Optical Genome Mapping Helps To Identify Bcr::Jak2 Rearrangement Arising From Cryptic Complex Chromosomal Aberrations: A Case Report And Literature Review, Neelam Vanjari, Guilin Tang, Gokce A Toruner, Wei Wang, Beenu Thakral, Ming Zhao, Bhavana J Dave, Joseph D Khoury, L Jeffrey Medeiros, Zhenya Tang

Faculty, Staff and Student Publications

We report a case of myeloproliferative neoplasm, not otherwise specified (MPN-NOS)-transformed AML with BCR::JAK2 rearrangement. Chromosomal analysis indicated a simple abnormal karyotype 46,XY,t(7;17)(q21;q24),t(9;22)(p24;q11.2). Fluorescence in situ hybridization (FISH) using a BCR/ABL1/ASS1 probe set suggested a possible BCR rearrangement and a reflex JAK2 breakapart probe indicated JAK2 rearrangement, most likely partnered with BCR. Optical genome mapping (OGM) analysis confirmed BCR::JAK2 derived through an inv(9)(p24p13) after a t(9;22)(p13;q11.2) in this case. Due to the complexity of chromosomal aberrations, disruption and/or rearrangement of other genes such as KIF24::BCR, JAK2::KIF24/UBAP1, and CDK6:SOX9 were also identified by OGM. Although the functionality and clinical …

Rad54l2-Mediated Dna Damage Avoidance Pathway Specifically Preserves Genome Integrity In Response To Topoisomerase 2 Poisons, Huimin Zhang, Yun Xiong, Yilun Sun, Jeong-Min Park, Dan Su, Xu Feng, Sarah Keast, Mengfan Tang, Min Huang, Chao Wang, Mrinal Srivastava, Chang Yang, Dandan Zhu, Zhen Chen, Siting Li, Ling Yin, Yves Pommier, Junjie Chen

Faculty, Staff and Student Publications

Type II topoisomerases (TOP2) form transient TOP2 cleavage complexes (TOP2ccs) during their catalytic cycle to relieve topological stress. TOP2ccs are covalently linked TOP2-DNA intermediates that are reversible but can be trapped by TOP2 poisons. Trapped TOP2ccs block transactions on DNA and generate genotoxic stress, which are the mechanisms of action of TOP2 poisons. How cells avoid TOP2cc accumulation remains largely unknown. In this study, we uncovered RAD54 like 2 (RAD54L2) as a key factor that mediates a TOP2-specific DNA damage avoidance pathway. RAD54L2 deficiency conferred unique sensitivity to treatment with TOP2 poisons. RAD54L2 interacted with TOP2A/TOP2B and ZATT/ZNF451 and promoted …

The Defined Tlr3 Agonist, Nexavant, Exhibits Anti-Cancer Efficacy And Potentiates Anti-Pd-1 Antibody Therapy By Enhancing Immune Cell Infiltration, Seung-Hwan Lee, Young-Ho Choi, Soon Myung Kang, Min-Gyu Lee, Arnaud Debin, Eric Perouzel, Seung-Beom Hong, Dong-Ho Kim

Faculty, Staff and Student Publications

Nexavant was reported as an alternative to the TLR3 agonist of Poly(I:C) and its derivatives. The physicochemical properties, signaling pathways, anti-cancer effects, and mechanisms of Nexavant were investigated. The distinctive characteristics of Nexavant compared to that of Poly(I:C) were demonstrated by precise quantification, enhanced thermostability, and increased resistance to RNase A. Unlike Poly(I:C), which activates TLR3, RIG-I, and MDA5, Nexavant stimulates signaling through TLR3 and RIG-I but not through MDA5. Compared to Poly(I:C), an intratumoral Nexavant treatment led to a unique immune response, immune cell infiltration, and suppression of tumor growth in various animal cancer models. Nexavant therapy outperformed anti-PD-1 …

Phenotyping Emt And Met Cellular States In Lung Cancer Patient Liquid Biopsies At A Personalized Level Using Mass Cytometry, Loukia G Karacosta, Danny Pancirer, Jordan S Preiss, Jalen A Benson, Winston Trope, Joseph B Shrager, Arthur Wai Sung, Joel W Neal, Sean C Bendall, Heather Wakelee, Sylvia K Plevritis

Faculty, Staff and Student Publications

Malignant pleural effusions (MPEs) can be utilized as liquid biopsy for phenotyping malignant cells and for precision immunotherapy, yet MPEs are inadequately studied at the single-cell proteomic level. Here we leverage mass cytometry to interrogate immune and epithelial cellular profiles of primary tumors and pleural effusions (PEs) from early and late-stage non-small cell lung cancer (NSCLC) patients, with the goal of assessing epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) states in patient specimens. By using the EMT-MET reference map PHENOSTAMP, we observe a variety of EMT states in cytokeratin positive (CK+) cells, and report for the first time MET-enriched CK+ …

Effects Of Independent Versus Dependent Stressful Life Events On Major Symptom Domains Of Schizophrenia, Yizhou Ma, Joshua Chiappelli, Mark D Kvarta, Heather Bruce, Andrew Van Der Vaart, Eric L Goldwaser, Xiaoming Du, Hemalatha Sampath, Samantha Lightner, Jane Endres, Akram Yusuf, Alexa Yuen, Samantha Narvaez, Danny Campos-Saravia, Peter Kochunov, L Elliot Hong

Faculty, Staff and Student Publications

We evaluated two models to link stressful life events (SLEs) with the psychopathology of schizophrenia spectrum disorders (SSD). We separated SLEs into independent (iSLEs, unlikely influenced by one's behavior) and dependent (dSLEs, likely influenced by one's behavior). Stress-diathesis and stress generation models were evaluated for the relationship between total, i- and d- SLEs and the severity of positive, negative, and depressive symptoms in participants with SSD. Participants with SSD (n = 286; 196 males; age = 37.5 ± 13.5 years) and community controls (n = 121; 83 males; 35.4 ± 13.9 years) completed self-report of lifetime negative total, i- and …

Ewsr1::Atf1 Orchestrates The Clear Cell Sarcoma Transcriptome In Human Tumors And A Mouse Genetic Model, Benjamin B Ozenberger, Li Li, Emily R Wilson, Alexander J Lazar, Jared J Barrott, Kevin B Jones

Faculty, Staff and Student Publications

Clear cell sarcoma (CCS) is a rare, aggressive malignancy that most frequently arises in the soft tissues of the extremities. It is defined and driven by expression of one member of a family of related translocation-generated fusion oncogenes, the most common of which is EWSR1::ATF1. The EWSR1::ATF1 fusion oncoprotein reprograms transcription. However, the binding distribution of EWSR1::ATF1 across the genome and its target genes remain unclear. Here, we interrogated the genomic distribution of V5-tagged EWSR1::ATF1 in tumors it had induced upon expression in mice that also recapitulated the transcriptome of human CCS. ChIP-sequencing of V5-EWSR1::ATF1 identified previously unreported motifs …

Novel Sox10 Indel Mutations Drive Schwannomas Through Impaired Transactivation Of Myelination Gene Programs, Erik A Williams, Ajay Ravindranathan, Rohit Gupta, Nicholas O Stevers, Abigail K Suwala, Chibo Hong, Somang Kim, Jimmy Bo Yuan, Jasper Wu, Jairo Barreto, Calixto-Hope G Lucas, Emily Chan, Melike Pekmezci, Philip E Leboit, Thaddeus Mully, Arie Perry, Andrew Bollen, Jessica Van Ziffle, W Patrick Devine, Alyssa T Reddy, Nalin Gupta, Kristen M Basnet, Robert J B Macaulay, Patrick Malafronte, Han Lee, William H Yong, Kevin Jon Williams, Tareq A Juratli, Douglas A Mata, Richard S P Huang, Matthew C Hiemenz, Dean C Pavlick, Garrett M Frampton, Tyler Janovitz, Jeffrey S Ross, Susan M Chang, Mitchel S Berger, Line Jacques, Jun S Song, Joseph F Costello, David A Solomon

Faculty, Staff and Student Publications

BACKGROUND: Schwannomas are common peripheral nerve sheath tumors that can cause severe morbidity given their stereotypic intracranial and paraspinal locations. Similar to many solid tumors, schwannomas and other nerve sheath tumors are primarily thought to arise due to aberrant hyperactivation of the RAS growth factor signaling pathway. Here, we sought to further define the molecular pathogenesis of schwannomas.

METHODS: We performed comprehensive genomic profiling on a cohort of 96 human schwannomas, as well as DNA methylation profiling on a subset. Functional studies including RNA sequencing, chromatin immunoprecipitation-DNA sequencing, electrophoretic mobility shift assay, and luciferase reporter assays were performed in a …

"Social" Movements: A Trend Analysis Of The Role Of Social Media In Social Movements, Courtney Nelson Stubbs

Theses and Dissertations

The gay rights movement has been very active on social media throughout the years. Using a trend analysis this study aimed to answer how social media is being used during a social movement, how a social movement evolves on social media, and how social media is being used by organizations as a public relations strategy to create change in social movements. Overall, the findings revealed 11 different ways social media is being used during a social movement, which shows how important opinion leaders are in helping a social movement gain traction and create the desired impact.

Perioperative Quality Initiative (Poqi) Consensus Statement On Perioperative Assessment Of Right Ventricular Function, Stephanie O Ibekwe, Jean Deschamps, Michael P W Grocott, Yafen Liang, Andrew Shaw, Tjorvi E Perry

Faculty, Staff and Student Publications

BACKGROUND: The right ventricle (RV) plays a central role in the maintenance of effective cardiac pump function. Despite overwhelming evidence that perioperative RV dysfunction (RVD) and failure (RVF) are associated with poor clinical outcomes, there are very few published recommendations or guidelines for comprehensive, evidence-based RV assessment on the risk of developing either during the perioperative period.

MAIN TEXT: To address this gap, the Perioperative Quality Initiative-IX (POQI-IX) investigators group, comprised of clinical experts in anesthesiology, cardiovascular surgery, internal medicine, critical care medicine, and advanced practice nursing, has developed a consensus statement based on current literature, published society recommendations, and …

Benzodioxane-Benzamides As Ftsz Inhibitors: Effects Of Linker's Functionalization On Gram-Positive Antimicrobial Activity, Lorenzo Suigo, William Margolin, Eugenia Ulzurrun, Martina Hrast Rambaher, Carlo Zanotto, Victor Sebastián-Pérez, Nuria E Campillo, Valentina Straniero, Ermanno Valoti

Faculty, Staff and Student Publications

FtsZ is an essential bacterial protein abundantly studied as a novel and promising target for antimicrobials. FtsZ is highly conserved among bacteria and mycobacteria, and it is crucial for the correct outcome of the cell division process, as it is responsible for the division of the parent bacterial cell into two daughter cells. In recent years, the benzodioxane-benzamide class has emerged as very promising and capable of targeting both Gram-positive and Gram-negative FtsZs. In this study, we explored the effect of including a substituent on the ethylenic linker between the two main moieties on the antimicrobial activity and pharmacokinetic properties. …

Nicotinic Acetylcholine Receptor Signaling Maintains Epithelial Barrier Integrity, Nadja S Katheder, Kristen C Browder, Diana Chang, Ann De Maziere, Pekka Kujala, Suzanne Van Dijk, Judith Klumperman, Tzu-Chiao Lu, Hongjie Li, Zijuan Lai, Dewakar Sangaraju, Heinrich Jasper

Faculty, Staff and Students Publications

Disruption of epithelial barriers is a common disease manifestation in chronic degenerative diseases of the airways, lung, and intestine. Extensive human genetic studies have identified risk loci in such diseases, including in chronic obstructive pulmonary disease (COPD) and inflammatory bowel diseases. The genes associated with these loci have not fully been determined, and functional characterization of such genes requires extensive studies in model organisms. Here, we report the results of a screen in

Uncontrolled Hypertension At The Dentist: A Case Report Of Integrated Healthcare, Amazon Doble, Raul Bescos, Robert Witton, Shabir Shivji, Zoë Brookes

Peninsula Dental School

Hypertension is a risk factor for major cardiovascular events and it is usually detected and managed by general medical practitioners (GPs) in primary care. However, it is estimated that 4.8 million adults are living with untreated high blood pressure (BP) in the UK. Health authorities are encouraging more collaborative work across health professions to find and refer individuals with undiagnosed hypertension. In this case, in 2022, a 65-year-old man with a previous history of hypertension, taking antihypertensive medication, attended a BP clinic at the University of Plymouth, Peninsula Dental School as part of a hypertension case finding pilot. His systolic …

Standardization Of An Onboarding Process, Kim Diaz, Lauren Machin, Karen Garcia Tunon

All Publications

No abstract provided.

How A Study Abroad Program Supports Its Students Of Color: A Case Study, Hannah E. L. Bloom

Master's Theses

Students of Color study abroad at rates lower than their white peers, thus missing out on the academic, personal, and intercultural benefits one can gain from studying abroad. The purpose of this thesis was to conduct a qualitative case study to examine how a study abroad program supports its Students of Color during the pre-departure phase of study abroad. This case study uses Critical Race Theory as its theoretical lens. Research for this case study included interviews with four study abroad practitioners from one study abroad program, and a review of the study abroad program’s online spaces. This thesis endeavored …

The Effects Of Intermittent Fasting On Female Athletes: A Systematic Review, Courtnie Scarbrough

Theses and Dissertations

The intermittent fasting diet has a historical past, but recently, it has regained popularity as a weight loss strategy for the general public. Athletes have also picked up this specialized diet in search of a competitive edge. There are ample studies testing athletes of all levels, but most do not include females or report sex-specific results. This systematic review aimed to identify current research on the effects of intermittent fasting in the understudied population of female athletes. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a comprehensive search was conducted in three databases, including PubMed, Cochrane, …

A Thematic Analysis Of Religious Identity And Volunteer Motivation Among Emerging Adult Members Of The Church Of Jesus Christ Of Latter-Day Saints, Jennifer R. Smith

Theses and Dissertations

This study sought insight into the religious and cultural identity of emerging adults belonging to the Church of Jesus Christ of latter-day Saints(LDS) and the impact such identity had in relation to volunteering motivation. Participants included 24 emerging adult college students belonging to the Church. Thematic analysis was used to determine influencing factors that led to Church membership and how Church membership shaped self-perception. Participants shared volunteer experiences and motivation to volunteer in relation to Deci and Ryan’s (2000) self-determination theory (SDT) was examined. The motivation of relatedness was identified among 100% of participants as compared to competence (37.5%) and …

Hypothalamic Manipulation Of The Anuran Hpg Axis: Alternative Hormones And A Non-Invasive Administration Route For Amphibian Assisted Reproductive Technologies (Art), Erin Michelle Saylor

Theses and Dissertations

Amidst the amphibian extinction crisis, in situ and ex situ amphibian species conservation initiatives utilize assisted reproductive technologies for optimal genetic management of captive and wild populations. Development of effective, simple, low-cost methods for obtaining gametes for artificial fertilization, sperm biobanking, or natural breeding alleviates obstacles for institutions or programs to apply reproductive technologies. Objectives herein include investigating the efficacy of alternative hormones for inducing spermiation, an alternative hormone administration route for inducing ovulation, exploring the physiological effects of hormone therapy, and utilizing sperm from deceased animals for biobanking.

The Experiences Of Teachers In Predominantly Black Schools, Kennetra Smith

Theses and Dissertations

Teachers want to be in schools where their identities are authentically affirmed. However, some educators in predominantly Black schools perceive that they are not always afforded the same level of value and respect as their colleagues in mostly White or mixed schools. Instead, teachers in predominantly Black schools seem to have experiences associated with frustrations that often go unheard of or dismissed for various reasons. Schools with predominantly Black student populations are often associated with challenges in retaining teachers, commonly referred to as teacher retention or teacher turnover. This turnover can have an impact on the academic progress of the …

Development Of An Automated Delivery System To Apply Copper Sulfate Crystals Using Precision Dry Fertilizer Application Technology, Kevin Charles Wise

Theses and Dissertations

The digenetic trematode, Bolbophorus damnificus, poses a substantial threat to catfish aquaculture, causing significant economic losses. Infestations lead to suppressed feed consumption, secondary bacterial infections and poor production performance. Survey data reveals widespread infestation in the in the southeastern United States. Current control strategies involve the application of a concentrated copper sulfate solution to reduce snail populations which serve as the first intermediate host of the trematode life cycle. This study aimed to improve treatment efficacy by developing a granular copper sulfate application system. A modified Gandy fertilizer applicator, equipped with a programmable control system, demonstrated accurate distribution of copper …

Examining The Impact A Bus Driver Attendance Incentive Program Had On Bus Driver Attendance In A Rural Mississippi School District, Samuel B. Allison

Theses and Dissertations

This study examines the impact of a bus driver attendance incentive program implemented in a rural Mississippi school district with the aim of improving bus driver attendance rates. There is a shortage of bus drivers across Mississippi which has made bus driver attendance paramount. Bus driver absenteeism causes disruptions in student transportation services and affects overall school operations. To address this problem, a rural Mississippi school district introduced a bus driver attendance incentive program that provided financial incentives for bus drivers who maintained perfect attendance rates each month.

The research methodology involved collecting and analyzing attendance data for bus drivers …

Investigation And Control Of Görtler Vortices In High-Speed Flows, Omar Es-Sahli

Theses and Dissertations

High-amplitude freestream turbulence and surface roughness elements can excite a laminar boundary-layer flow sufficiently enough to cause streamwise-oriented vortices to develop. These vortices resemble elongated streaks having alternate spanwise variations of the streamwise velocity. Following the transient growth phase, the fully developed vortex structures downstream undergo an inviscid secondary instability mechanism and, ultimately, transition to turbulence. This mechanism becomes much more complicated in high-speed boundary layer flows due to compressibility and thermal effects, which become more significant for higher Mach numbers. In this research, we formulate and test an optimal control algorithm to suppress the growth rate of the aforementioned …

Scalable And Explainable Self-Supervised Motif Discovery In Temporal Data, Somayeh Bakhtiari Ramezani

Theses and Dissertations

The availability of a scalable and explainable rule extraction technique via motif discovery is crucial for identifying the health states of a system. Such a technique can enable the creation of a repository of normal and abnormal states of the system and identify the system’s state as we receive data. In complex systems such as ECG, each activity session can consist of a long sequence of motifs that form different global structures. As a result, applying machine learning algorithms without first identifying the local patterns is not feasible and would result in low performance. Thus, extracting unique local motifs and …

Examining The Impact That A Modified School Calendar Had On Teacher Attendance In A Rural Mississippi School District, James W. Carlisle Jr.

Theses and Dissertations

Educational leaders have long recognized the certified classroom teacher as the most important factor in student performance and success. In his research article Teachers Matter: Understanding Teachers’ Impact on Student Achievement for the Rand Corporation, Opper (2019) stated, “When it comes to student performance on reading and math tests, teachers are estimated to have two to three times the effect of any other school factor, including services, facilities, and leadership” (para. 2). While district and school administrators certainly understand the impact teachers have on student performance, little has been done to mitigate the issue of teacher absenteeism, which has become …

The Impact Of Prior Pandemic Planning On Health Outcomes During The Covid-19 Pandemic: A Study Of Local Government Pandemic Planning In The United States, Karissa D. Bergene

Theses and Dissertations

In late 2019, scientists discovered a novel strain of coronavirus originating out of Wuhan, China. By January 2020, the United States (U.S.) saw its first positive case of coronavirus and the public was still unsure how serious of an epidemic this would be. By the time the World Health Organization declared the novel coronavirus disease (COVID-19) epidemic a “global pandemic” on March 11, 2020, world leaders, public health experts, scientists, and public administrators kicked into high gear to start serious pandemic response efforts. The public sector is typically the responsible authority for the preparedness, response, mitigation, and recovery of such …

Stream Water Quality Under Baseflow Conditions In A Livestock Production Area, Maryi Lorena Chavarro Chaux

Theses and Dissertations

This study evaluates the spatial and temporal impacts of implemented Best Management Practices (BMP) on stream water quality under baseflow conditions in a stream segment affected by livestock production. The BMPs includes 10-m fenced riparian zone and crossing paths along the reach segment. Grab water samples and water quality monitoring were collected biweekly from July 2019 to March 2022. Water quality parameters included temperature, dissolved oxygen, electric conductance, total dissolved solids, turbidity, and pH. Water samples were lab analyzed for sediments and nutrients. Results evidenced poor water quality before BMPs implementation, with nutrient concentrations exceeding the nation's and state's criteria. …

Advancing Air Filtration Analysis: A Comprehensive Approach To Particle Loading Models, Gentry Nathaniel Berry

Theses and Dissertations

Fibrous air filters are commonly used to capture airborne particles due to their potential for a relatively high capture efficiency and low airflow resistance. Their performance characteristics make them ideal candidates in many instances, spanning a wide range from residential to sensitive industrial applications. However, as more particles are captured, the performance of the filter will evolve. This evolution of performance typically manifests as a higher capture efficiency and higher airflow resistance resulting from the additional particulate deposits. The prediction of fibrous filter performance has been the focus of research for many decades, resulting in numerous analytical, numerical, and empirical …

Advancing Salamander Conservation Efforts In Zoos And Aquaria Through Assisted Reproductive Technologies (Art), Devin Marie Chen

Theses and Dissertations

Salamanders are one of the most at-risk taxa in the world due to habitat destruction, pollution, climate change, invasive diseases, and more. This has led to a need for conservation breeding programs that are often associated with zoos and aquaria. Salamanders can be difficult to breed in captivity, though, due to their dependence on specific environmental cues and other unknown factors that stimulate sperm and egg production. To overcome these challenges, assisted reproductive technologies (ART) such as exogenous hormone administration, sperm cryopreservation, and in-vitro fertilization have been developed to increase offspring propagation and maintain genetic diversity. If genetically robust populations …

We Built This Country For Free – Using A Phenomenological Approach For (Re) Imagining Mississippi Black Small-Scale Farmers, Destiny Denise Crockett

Theses and Dissertations

By the early 20th century, in 1920, Black farmers owned 14% of US farmland. Today, in the 21st century, Black farmers own less than 2% of US farmland. The demise of Black farmers and Black farmland in US Agriculture is a direct result of social, political, and racial weaponization against their foodways, culture, and livelihoods. The history concerning the plight of Black farmers goes beyond USDA's historical discrimination but enters a position where racism is embedded and perpetuated within the structure of US agriculture. In effect, Black small-scale farmers have reaped the downfall of this system, enduring racial biases and …

“I Tell It Like It Is, Then How It Could Be”: Public Discourse And Public Administration In Popular American Music Since The 1990s, Kristen Dechert

Theses and Dissertations

This study is an analysis of lyrics from popular American music albums since the 1990s to understand public discourse about and experience with public administration. Albums included in the study came from two prominent sources: Billboard and Pitchfork decade-end lists. The study is framed by critical, postmodern, and responsiveness theories, methodologically grounded in critical discourse analysis, and technically approached using standard qualitative methods, including especially qualitative content analysis and iterative, inductive coding techniques. Findings from the qualitative study demonstrate public discourse on the themes of institutional and systemic racism and violence within and perpetuated by public administration and public institutions. …