Digital Commons Network^™

Investigation Of Three Single-Nucleotide Polymorphisms (1252 G>A, 2534 G>A, And 2569 T>C) Of The Mbl1 Gene In Anatolian Buffaloes, Esma Gamze Aksel, Bi̇lal Akyüz

Turkish Journal of Veterinary & Animal Sciences

It has been reported that since the MBL gene is involved in the first step of infection and inflammation processes, it may be associated with susceptibility to bacterial and viral diseases in living organisms. In this study, it was aimed to determine the genotypic variation of Anatolian buffaloes in terms of three (1252G>A, 2534 G>A, and 2569 T>C) single nucleotide polymorphisms (SNPs) within the MBL-1 gene. For this purpose, 103 Anatolian buffaloes were genotyped for relevant SNPs by PCR-RFLP process. The allele and genotype frequencies of the examined samples were determined, and the population was examined for …

Effect Of Thermo-Insulated Kid Hutch On Location Preference, Growth Performance, And Blood Haemato-Biochemical Indices Of Winter-Born Goat Kids (Capra Hircus), Ajoy Das, Dilip Kumar Mandal, Asish Debbarma, Muthupalani Karunakaran, Anupam Chatterjee, Debajyoti Sarkar

Turkish Journal of Veterinary & Animal Sciences

This study assessed the effect of thermo-insulated kid hutch (TIKH) on location preference, body surface temperature, growth performance, and blood haemato-biochemical indices of winter-born Black Bengal goat kids. Does were maintained in the same shed, born kids (n = 12) were divided into two groups along with mothers to begin experiments from day 1 of birth. The treatment group (TIKH) of kids was given a TIKH to which kids had free access, but not the adults. However, in the control group (CONT) kids were reared without TIKH. The floor surface temperature of TIKH was significantly (p < 0.05) higher at night as compared to goat shed in December (4.28%), January (3.98%), and February (7.72%). The interior roof surface of TIKH had 25.12%, 33.55%, 31.85%, 10.99%, and 4.37% higher (p < 0.01) temperatures at night compared to the roof surface temperature of the goat shed in December, January, February, March, and April, respectively. The presence of kids inside the TIKH at the age of 1–15 days was the greatest in morning (80.5%) and evening (72.2%). Between the ages of 16–-60 days, the preference of kids for the TIKH varied from 69.2% to 54.2% in the morning and from 70.0% to 66.7% in the evening. However, at the age of 61–75 days, kids’ preference for TIKH gradually declined in the morning (11.6%) and evening (19.9%) due to the increased inside temperature. Kids weaning weight was 23.25% higher (p < 0.05) in the TIKH group as compared to that of CONT. The average daily gain (0–30 days) was higher (41.34%; p < 0.05) in kids in the TIKH compared to the CONT shed. The haemato-biochemical profiles of kids were similar in both groups except for blood serum glucose, which was greater (p < 0.05) in kids of the TIKH than that of CONT shed. In conclusion, the provision of TIKH improved the growth and overall performance of winter-born Black Bengal goat kids during pre and postweaning periods.

Water Buffalo Social Networks During Their Activities On Pasture Under The Rain, Eleni Tsiobani, Maria Yiakoulaki, Nikolaos Hasanagas, Ioannis Antoniou

Turkish Journal of Veterinary & Animal Sciences

Water buffaloes exhibit various social structures during their activities on pasture, but the impact of abiotic factors (i.e. rainfall) on these structures remains unknown. While existing research has provided evidence concerning the effect of weather conditions on grazing animals’ behavior, they have not examined how animals differentiate their social organization within the herd during their activities on pasture under the rainfall. This study utilized social network analysis to construct, depict, and analyze the proximity networks among buffaloes in each activity they perform on pasture before, during, and after the rainfall. By applying various network indices, we have shown that water …

Functional Training Promotes Benefits In The Physical Condition Of Horses, Paula Gomes Rodrigues, Camila Fernanda Fidêncio, Katia De Oliveira, Camilla Mendonça Silva, Clistenes Gomes Oliveira, José Miradelson Oliveira Carvalho, Anselmo Domingos Ferreira Santos, Gregorio Murilo De Oliveira Junior, José Camisão De Souza

Turkish Journal of Veterinary & Animal Sciences

Horses that perform constant physical activity related to sport or work are subject to musculoskeletal disorders. Thus, the objective was to evaluate the effectiveness of a functional training program to promote physical conditions (epaxial musculature biometry and linear kinematics) and behavior traits in patrol horses. Sixteen crossbred horses were randomly distributed in two experimental treatments: not submitted (control group) and submitted (test group) to a functional exercise program. The training program exercises were performed three times per week for 90 days and consisted of dynamic mobilization techniques, reflexive mobilization, dynamic imbalance, and passive stretching. The studied variables were Longissimus dorsi …

Radiology Versus Rt-Pcr For Detection Of Proventricular Dilatation Disease In Psittacines, Mohammad Bayat, Abolfazl Ghaniei, Majid Jamshidian Mojaver, Ali Mirshahi, Mohammad Azizzadeh

Turkish Journal of Veterinary & Animal Sciences

Proventricular dilatation disease (PDD) is a potentially fatal disease of psittacine birds. Avian bornavirus (ABV), the causative agent of PDD, causes inflammation of the central, peripheral, and autonomic nervous systems. Clinically, birds affected by PDD present with gastrointestinal tract dysfunction, neurological symptoms, or both. In this study, we evaluated the presence of proventriculus dilation and ABV in 40 birds (cockatiel, n = 12; budgerigar, n = 28), submitted to the veterinary clinic, by radiology and RT-PCR. Lateral and ventrodorsal radiographs and feather samples were taken from 36 live birds including the cockatiel and budgerigar, as well as samples of proventriculus …

Prevalence Of Carriers Of Cholesterol Deficiency–Associated Apob Mutation In Some Native, Holstein And Simmental Cattle Breeds In Türkiye, Hasan Meydan, Kübra Bedi̇r Di̇bi̇ç, Onur Vural, Mehmet Ali̇ Yildiz, Jørgen S. Agerholm

Turkish Journal of Veterinary & Animal Sciences

Genetic diseases play an increasingly important role in cattle breeding as breeding technologies such as artificial insemination has an inborn risk of disseminating deleterious genes, especially from high merit breeding sires. Cholesterol deficiency (CD) is a metabolic disorder first recognized in Holstein cattle in 2015. The condition is characterized by chronic diarrhea and unthriftiness in calves being homozygous for the defective allele. The prevalence of carriers of CD in Turkish cattle is at present unknown and therefore 700 heifers and cows of different breeds, including 300 Holsteins were genotyped. Of the tested Holsteins, 2.0% turned out to be carriers of …

Virological And Pathological Investigation Of Canine Parvovirus-2 (Cpv-2) With The Assessment Of The Genetic Variability Of Field Strains, Zeynep Karapinar, Musa Karaman, İhsan Kisadere, Mustafa Usta, Mehmet Özkan Ti̇murkan

Turkish Journal of Veterinary & Animal Sciences

Canine parvovirus-2 (CPV-2), which is common worldwide, is a highly contagious pathogen in domestic dogs and some wild carnivore species. The agent causes a multisystemic infection in affected animals, especially acute hemorrhagic enteritis and fatal myocarditis in puppies. The study aimed to reveal the presence of virus in dogs with clinical symptoms of CPV-2 infection using virological, pathological, and immunohistochemical methods and make molecular characterization based on partial VP-2 gene of strains circulating in the region. As a result of PCR studies, 9 out of 16 rectal swab samples and 17 out of 38 blood samples were positive. A phylogenetic …

Validation Of A Chimeric Proteins-Based Elisa For The Diagnosis Of Brucellosis In Kazakhstan, Aitbay Bulashev, Saule Eskendirova, Bakytkali Ingirbay, Alfiya Syzdykova, Fariza Zhagipar

Turkish Journal of Veterinary & Animal Sciences

This study aimed to compare the suitability of three chimeric recombinant outer membrane proteins of Brucella (rOmps 19 + 25, 19 + 31, and 25 + 31) for the diagnosis of bovine and sheep brucellosis using an indirect ELISA (i-ELISA). The diagnostic properties of these proteins in an i-ELISA were studied using blood serum samples from cattle and sheep with known positive and negative results for brucellosis based on the rose Bengal plate test (RBT), complement fixation test (CFT), and a commercial ELISA (c-ELISA) kit, as well as using animal sera from fresh foci of infection with unknown reactivity. rOmps19 …

Ddx219.3 Social Media Content Creation Example 1, Sae University College

Exemplars

Social Media Marketing and Content Creation

Impacts Of Artificial Light At Night On The Early Life History Of Two Ecosystem Engineers, Svenja Tidau, Fraser T. Brough, Luis Gimenez, Stuart R. Jenkins, Thomas W. Davies

School of Biological and Marine Sciences

Sessile marine invertebrates play a vital role as ecosystem engineers and in benthic-pelagic coupling. Most benthic fauna develop through larval stages and the importance of natural light cycles for larval biology and ecology is long-established. Natural light-dark cycles regulate two of the largest ocean-scale processes that are fundamental to larvae’s life cycle: the timing of broadcast spawning for successful fertilisation and diel vertical migration for foraging and predator avoidance. Given the reliance on light and the ecological role of larvae, surprisingly little is known about the impacts of artificial light at night (ALAN) on the early life history of habitat-forming …

School Improvement Tool Elaborations: Student Engagement And Wellbeing. Background Report And Literature Review, Fabienne Van Der Kleij, Pauline Taylor-Guy, Tanya Vaughan, Marijne Medhurst, Christina Rogers

School and system improvement

This literature review outlines the evidence that underpins the development of a set of evidence-informed elaborations, or specific practices, that support student engagement and wellbeing across the 9 domains of the National School Improvement Tool (NSIT). These observable, measurable practices to support student engagement and wellbeing have been developed from a review of relevant literature in the areas of socio-emotional learning (SEL), health and wellbeing, student engagement and a sense of belonging, and motivations for engagement. They also take account of commissioned research reports by the Queensland Department of Education and the Department's (2018) approach to student learning and wellbeing …

An Overview Of Human Trafficking In The United States, Lin Welsh

Theses

This research paper delves into the multifaceted problem of human trafficking, seeking to provide a comprehensive understanding of its dynamics, root causes, and the myriad challenges associated with combating this serious crime. Drawing on empirical studies and criminological theories, the paper navigates through the factors that contribute to the perpetuation of human trafficking. The study begins by exploring the historical and theoretical contexts that create the demand for trafficked persons and the vulnerabilities that make individuals susceptible to exploitation. Additionally, the paper examines the legal and policy frameworks implemented at the national level to combat human trafficking. It aims to …

Streptococcus Intermedius: From A Normal Oral Commensal To A Life-Threatening Organism, Anas Mahmoud, Tala Beilani, Nizar Alyassin, Karam Zakharia, Taha Basil, David Poulad

Student Publications

Subdural empyema is a collection of pus in the subdural space between the dura mater and the arachnoid. It carries very high morbidity and mortality as it can spread anywhere in the brain; however, the risk can be mitigated with appropriate surgical and medical intervention. Being protected by the skull, cranial infections are usually preceded by a significant risk factor, either an external invader such as skull fractures secondary to trauma, penetrating injury, prior surgery, or, more commonly, in more than 50% of cases, due to spread of an internal infection such as ear or sinus infections. Anaerobic and aerobic …

Experiences Of Caregivers Of People With Dementia In Indonesia: A Focus Group Study, Imelda Theresia, Tara P. Sani, S Evans‐Lacko, Nicolas Farina, Lydia Augustina, Yuda Turana

Peninsula Medical School

Abstract Background Many people with dementia are reliant on family caregivers to provide daily care to maintain quality of life and dignity. As a result, caregivers can experience increased burden, poorer health outcomes and increased stigma. To date, the experiences of caregivers of people with dementia has not been explored within an Indonesian context. Aims This study aims to understand the experience of caregivers of people with dementia in Indonesia and better understanding of the stigma associated with dementia. Materials and Methods This qualitative study is embedded within the Strengthening Responses to Dementia in Developing Countries project. Focus Group Discussions …

The Western Channel Observatory: A Century Of Physical, Chemical And Biological Data Compiled From Pelagic And Benthic Habitats In The Western English Channel, Andrea J. Mcevoy, Angus Atkinson, Ruth L. Airs, Rachel Brittain, Ian Brown, Elaine S. Fileman, Helen S. Findlay, Caroline L. Mcneill, Clare Ostle, Tim J. Smyth, Paul J. Somerfield, Karen Tait, Glen A. Tarran, Simon Thomas, Claire E. Widdicombe, E. Malcolm S. Woodward, Amanda Beesley, David V.P. Conway, James Fishwick, Hannah Haines, Carolyn Harris, Roger Harris, Pierre Hélaouët, David Johns, Penelope K. Lindeque, Thomas Mesher, Abigail Mcquatters-Gollop, Joana Nunes, Frances Perry, Ana M. Queiros, Andrew Rees, Saskia Rühl, David Sims, Ricardo Torres, Stephen Widdicombe

School of Biological and Marine Sciences

The Western Channel Observatory (WCO) comprises a series of pelagic, benthic and atmospheric sampling sites within 40 km of Plymouth, UK, that have been sampled by the Plymouth institutes on a regular basis since 1903. This longevity of recording and the high frequency of observations provide a unique combination of data; for example temperature data were first collected in 1903, and the reference station L4, where nearly 400 planktonic taxa have been enumerated, has been sampled on a weekly basis since 1988. While the component datasets have been archived, here we provide the first summary database bringing together a wide …

Vodka2: A Fast And Accurate Method To Detect Non-Standard Viral Genomes From Large Rna-Seq Data Sets, Emna Achouri, Sébastien A Felt, Matthew Hackbart, Nicole S Rivera-Espinal, Carolina B López

2020-Current year OA Pubs

During viral replication, viruses carrying an RNA genome produce non-standard viral genomes (nsVGs), including copy-back viral genomes (cbVGs) and deletion viral genomes (delVGs), that play a crucial role in regulating viral replication and pathogenesis. Because of their critical roles in determining the outcome of RNA virus infections, the study of nsVGs has flourished in recent years, exposing a need for bioinformatic tools that can accurately identify them within next-generation sequencing data obtained from infected samples. Here, we present our data analysis pipeline, Viral Opensource DVG Key Algorithm 2 (VODKA2), that is optimized to run on a parallel computing environment for …

Engineering E. Coli Strains Using Antibiotic-Resistance-Gene-Free Plasmids, Matthew B Amrofell, Sunaina Rengarajan, Steven T Vo, Erick S Ramirez Tovar, Larissa Lobello, Gautam Dantas, Tae Seok Moon

2020-Current year OA Pubs

We created a generalizable pipeline for antibiotic-resistance-gene-free plasmid (ARGFP)-based cloning using a dual auxotrophic- and essential-gene-based selection strategy. We use auxotrophic selection to construct plasmids in engineered E. coli DH10B cloning strains and both auxotrophic- and essential-gene-based selection to (1) select for recombinant strains and (2) maintain a plasmid in E. coli Nissle 1917, a common chassis for engineered probiotic applications, and E. coli MG1655, the laboratory "wild-type" E. coli strain. We show that our approach has comparable efficiency to that of antibiotic-resistance-gene-based cloning. We also show that the double-knockout Nissle and MG1655 strains are simple to transform with plasmids …

A Comparison Of Depressive Symptom Self-Reported Measures In The Texas Youth Depression And Suicide Research Network (Tx-Ydsrn), Karabi Nandy, A John Rush, Thomas Carmody, Beth D Kennard, Graham J Emslie, Holli Slater, Taryn L Mayes, Melissa Defilippis, Cynthia Garza, Eric A Storch, Sarah M Wakefield, Madhukar H Trivedi

Faculty, Staff and Students Publications

Objective: To evaluate psychometrically and provide crosswalks between 3 self-report measures of depressive symptomatology in youth in psychiatric care settings. Ratings included the Patient Health Questionnaire for Adolescents (PHQ-A), a widely used 9-item self-report; the 16-item Quick Inventory of Depressive Symptomatology-Self-Report (QIDS-SR16); and the 5-item Very Quick Inventory of Depressive Symptomatology-Self-Report (VQIDS-SR5), a recent effort to create a bridge from the QIDS-SR16 to clinical practice.

Methods: Data from the Texas Youth Depression and Suicide Research Network Registry (August 26, 2020-May 11, 2022) were included in this work. At first visit, 795 depressed or suicidal adolescent (12-20 years of age) psychiatric …

Safety And Immunogenicity Of An In Vivo Muscle Electroporation Delivery System For Dna-Hsp65 Tuberculosis Vaccine In Cynomolgus Monkeys, Monique Ribeiro De Lima, Ana C. Leandro, Andreia Lamoglia De Souza, Marcio Mantuano Barradas, Eric Henrique Roma, Ana Teresa Gomes Fernandes, Gabrielle Galdino-Silva, Joyce Katiuccia M. Ramos Carvalho, Renato Sergio Marchevsky, John L. Vandeberg

School of Medicine Publications

A Bacille Calmette–Guérin (BCG) is still the only licensed vaccine for the prevention of tuberculosis, providing limited protection against Mycobacterium tuberculosis infection in adulthood. New advances in the delivery of DNA vaccines by electroporation have been made in the past decade. We evaluated the safety and immunogenicity of the DNA-hsp65 vaccine administered by intramuscular electroporation (EP) in cynomolgus macaques. Animals received three doses of DNA-hsp65 at 30-day intervals. We demonstrated that intramuscular electroporated DNA-hsp65 vaccine immunization of cynomolgus macaques was safe, and there were no vaccine-related effects on hematological, renal, or hepatic profiles, compared to the pre-vaccination parameters. No tuberculin …

Challenges And Opportunities In Insulin Resistance-Induced Cognitive Dysfunction, Nancy Abdel-Kareem, Shimaa Elshazly, May Galal, Enas Ahmed Abd El-Haleim

Bulletin of Faculty of Pharmacy Cairo University

In the last few years, there has been a lot of interest in the effect of nutrition on cognitive function, as it affects insulin signals inside the body, whose role is not limited to controlling blood glucose levels but also extends to the learning process and cognitive function. Nutrition-induced insulin resistance is one of the present diseases, which impairs brain insulin signaling and leads to various forms of dementia. This review discusses the different roles of insulin inside the body, the negative effect of insulin resistance on brain function, and the use of some drugs that may improve cognitive dysfunction.

Conflict And Choice Of Study At University Level: Evidence From Pakistan, Abbas Ali Gillani, Xiaocheng Hu

Peace and Conflict Studies

Conflict, and violence related events have been found to have significant effects on the cognitive thinking and mental well-being of individuals. Although there is ample evidence suggesting negative association of conflict with schooling outcomes, there is non-existent research on how violence can impact degree choices made by students at the university level. By using university level admissions data between 2014 and 2016 from Pakistan, this paper examines the differential in preference for degree choices of students who live in conflict-affected areas compared to students who live in conflict-free areas. The results show that students exposed to violence were less likely …

Food And Commensality In Non-State Armed Groups: The Case Of The Lord's Resistance Army In Northern Uganda, 1987-2008, Eunice Otuko Apio Dr

Peace and Conflict Studies

The subfield of food and eating practices has registered a significant volume of theoretical and empirical studies. However, there is very limited research targeting non-state armed groups. This article contributes to understanding the nuanced role of food and eating practices (or commensality) in conflict, and its significance in the construction and sustenance of sense of community in non-state armed groups that use particularly elaborate means of indoctrination to build a following. Drawing on the case of northern Uganda’s Lord’s Resistance Army (LRA) that thrived on mass abduction of children, youth and women between 1987 and 2008, this article argues that …

Student Perception Of Crime And The Role Of School Resource Officers, Olivia Dunn

Honors Program Theses and Projects

The increased use of school safety measures and Zero tolerance policies in the United States education system has created a disconnect between perceived and actual school safety. While legislators, school administrators, and the public fuel the creation of school safety guidelines, the voice of the student population these guidelines impact may have been lost. The purpose of this study was to investigate student perceptions of crime, and the role of School Resource Officers (SROs) based on their high school experience. This project was conducted to help understand the factors that contribute to student perceptions and how school security measures may …

Influence Of Gender Identity On The Adoption Of Religious-Spiritual, Preventive And Emotion-Focused Coping Strategies During The Covid-19 Pandemic In Pakistan, Saeed Ahmad, Sara Rizvi Jafree

Sociology and Anthropology Student Research

Background

Academic research has highlighted the gendered impacts and amplifications of gender disparities of COVID-19. Traditionally, Pakistan is a patriarchal society, where it is a parenthood norm to socialize specific gender social roles.

Objectives

The current research asserts that these normative gender roles may influence individuals throughout their life course, even during the COVID-19 pandemic. Therefore, the present study explored the influence of gender identity in adopting different coping strategies such as religious-spiritual, preventive, emotion-focused and non-constructive coping against the COVID-19 pandemic.

Methods

Due to the lockdown in various areas of Pakistan, data were collected through an online questionnaire using …

Nursing Care Of The Pediatric Patient With Autism, Cayley Frolik

Honors Capstones

The purpose of this project is to help educate current and future nurses on ways to understand and better connect with pediatric patients with autism. Standard nursing curriculum tends to focus primarily on the clinical approach for mainstream patients. In one short year, I recognized a glaring need for nurses to learn how to care for those with autism, as well as how to support and educate their families to create the best environment for whole success. It is very difficult to understand the needs of these specific patients without the proper toolkit. It is my intention to research and …

Pollution Trapping By Strong Temperature Inversions In Fairbanks, Alaska, Meeta V. Cesler-Maloney

Chemistry and Biochemistry

In cold climates during winter, surface-based temperature inversions reduce vertical dispersion within the atmospheric boundary layer. Reduced vertical dispersion coupled with stagnant horizontal winds causes pollution emitted near the ground level to accumulate to unhealthy amounts. To study pollution trapping, we measured vertical differences in fine particulate matter and ozone across a shallow 20-meter vertical scale during surface-based inversions in Fairbanks, Alaska and showed that pollution trapping occurs on this extremely shallow scale. In winter 2022, trace gases were measured by long-path differential absorption spectroscopy to probe vertical dispersion on a taller scale, up to ~200 m above the surface. …

Indigenous Self-Determination In Co-Production Of Knowledge, Margaret Hope Rudolf

Anthropology

Analyzing and comprehending co-production of knowledge (CPK) in the context of working with Arctic Indigenous communities on climate change research is the main goal for this interdisciplinary doctoral dissertation. CPK is shared decision-making on every step of the research process with research partners from communities, agencies, or organizations. CPK with Arctic Indigenous communities requires dedicated consideration of equity, ethics, cultural worldviews, and colonization. Key concepts from Indigenous critical methodologies are used to analyze both the CPK theory and implementation. CPK has the potential to be an ethical space to question the status quo of research processes and support Indigenous self-determination. …

Uncovering Patterns And Mechanisms Of Paralytic Shellfish Toxicity In Alaska's Geoduck Clam Fishery, Courtney E. Hart

Fisheries

This dissertation explores the patterns and mechanisms of paralytic shellfish toxicity in commercially harvested geoduck clams caused by the toxin-producing dinoflagellate Alexandrium catenella in Southeast Alaska. Alaska's commercial fishery for geoduck clams (Panopea generosa) is a small but lucrative fishery, with annual ex-vessel values averaging US $3.9 million (2010-2022). In recent years, the presence of paralytic shellfish toxins (PSTs) in clam tissue resulted in declines in fishery openings and harvest. PSTs can bioaccumulate in the tissues of filter feeders when A. catenella blooms in the spring and summer seasons. However, high levels of toxicity in geoduck clams occur sporadically during …

Estimating Relative Indices Of Groundfish Abundance From Multiple Fishery-Independent Data Sources: A Comparison Of Intercalibrating Model-Based Abundance Estimators, Tristan Noble Sebens

Fisheries

Stock assessments are critical tools for sustainable fisheries management, and abundance indices estimated from fishery-independent data represent a crucial data source for these assessments. However, financial constraints on these surveys limit the number of samples taken per year, and/or the frequency with which regions are sampled. Additionally, it remains challenging for any individual survey to sample the entire domain of a stock due to contact-selectivity of the sampling gear and the accessibility of specific habitats to specific gear types. Further, the cost of operating fishery-independent surveys can result in surveys conducted on biennial or triennial schedules, resulting in temporal gaps …

Emotional Labor In Front-Facing Jobs In University Settings, Khushboo Jain

Student Theses and Dissertations

This research aims to investigate how employees in front-facing/public-oriented positions engage in and experience the effects of emotional labor. Emotional labor is the unpaid, frequently unseen work a person must perform to manage their emotions for other people (Resnick, 2021). This kind of labor can manifest in multiple settings such as the workplace and interpersonal relationships. Emotional labor is often a problem in organizations because there is more focus on how the employee is representing them in customer interactions than the employee’s well-being. During face-to-face or voice-to-voice interactions with customers, employees are required to express proper emotions as a job …