Digital Commons Network^™

Recent Advances In Crispr/Cas9-Assisted Gene Therapy, Apeksha Srivastava, Shikha Chauhan, Vishal Ahuja

Karbala International Journal of Modern Science

CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) is an exponentially growing tool with wide-spread applications in therapeutics like gene modifications that focus on altering the hereditary material to repair or eliminate any defective gene-causing diseases like cancer, AIDS (Acquired immunodeficiency syndrome), etc. It also includes the identification of the target sequence with the help of sgRNA followed by the substitution of a malfunction-ing gene with a normal version. It offers high efficiency, specificity, and post-gene-editing efficacy, but have also some off-target impressions, and immunogenic effects. The contribution of CRISPR/Cas9 has already been proved primarily in in-vitro studies using animal germ …

Estimation Of Water Quality Of Dikhu River Of Nagaland Through A Combination Of Water Quality Index And Principal Component Analysis Techniques., Lanuyanger Longchar, Wati Temjen, Khikeya Semy, Wati Lemla

Karbala International Journal of Modern Science

Monitoring the physico-chemical attributes of water is essential for determining water quality. The present study explores the effect of various anthropogenic factors on the quality of Dikhu River, Nagaland. Water samples from three sampling stations were compared against three standards: Indian Council of Medical Research, Bureau of Indian Standards, and World Health Organization. Although all parameters were within the permissible limits, the Water Quality Index categorized all sites during the rainy season as "poor quality", highlighting anthropogenic impacts. A principal component analysis created a Minimum Data Set (MDS) explaining 100%, 93.27%, and 96.26% of the total variance for the three …

Method For Visualising Energy Sources Powering Blockchain Transactions And Apparatus Thereof, Sean Terribilini

Defensive Publications Series

The present disclosure relates to a technique for providing visualization of energy sources powering the blockchain transactions. The technique involves determining the energy consumption pattern of each participating node during a transaction. The health of a card associated with a user is displayed by comparing the energy consumption associated with the blockchain transactions performed by the user with a pre-determined threshold. In one embodiment, the health of the card is determined based on the energy consumption report and carbon footprints associated with the transactions. Further, the technique further comprises triggering car linked offers (CLOs) for the user to offset the …

Spartan Daily, October 10, 2023, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2023

Volume 161, Issue 21

Chapter Ii — Survey Of Ethiopia’S Survival: Definition And Controversies, Messay Kebede

Ethiopian Modernization: Opportunities and Derailments

The last chapter has unraveled the close link between modernization and the emergence of a survival ethos among ruling elites of traditional societies in transition. To approach the case of Ethiopia’s modernization from the same angle of explanation, it is necessary first to make sure that some groups among Ethiopian ruling elites had shown a propensity that could be defined as a salvational will. Sure enough, any ruling elite wants to protect the social order that benefits it. However, the salvational will, as defined in the previous chapter, is less the desire to perpetuate the status quo than the will …

Supporting Students From Different Universities And Backgrounds To Improve Their Academic And Social Outcomes: Euniwell Masoee Project Workshop, Neil Cooke, Jörgen Forss, Enrica Caporali, Pascal Chargé, Kamel Hawwash, Jesper Andersson, Gianni Bartoli, Sarah Chung, Daniel Cottle

Workshops

There is a notable discrepancy between the relative prosperity of Europeans and the global security and sustainability challenge. The mission of the ERASMUS+ 2020 European University for Well-Being (EUniWell) alliance is to address this. Our project, “Maximizing Academic and Social Outcomes in Engineering Education” (MASOEE) interprets this contradiction for engineering educators, exploring how to ensure graduates make the utmost contribution to societal wellbeing by narrowing attainment gaps. We are combining the expertise of British, French, Italian, and Swedish faculties to identify, share, and ultimately transfer best practices for professional, business, and sustainability skill teaching that is aligned to the EU …

The Indiana Statesman, October 10, 2023, Indiana State University

Student Newspaper

Deborah Curtis retiring in 2024; Hardee’s workers stealing; Student Government Association promotes mental health awareness

Updating The Berne Convention For The Internet Age: Un-Blurring The Line Between United States And Foreign Copyrighted Works, Ethan Schow

Brigham Young University Journal of Public Law

John Naughton, notable journalist and academic, has asserted that “[common sense] should also revolt at the idea that doctrines about copyright that were shaped in a pre-Internet age should apply to a post-Internet one.” And yet, in crucial aspects of international law, this is the situation in which the world finds itself today. The Berne Convention for the Protection of Literary and Artistic Works (the “Berne Convention” or the “Convention”) is one of the most important multinational agreements concerned with copyright law, but it has not been amended since September 28, 1979. Although the internet technically existed in an early …

Getting Started – Hands-On Producing Lecture Films, Anja Pfennig

Workshops

Lecture videos are more and more implemented in higher engineering education to be used widely by students because very often literature only presents results but not how to get there. Lecture videos may close this gap and visualize the sometimes obvious but still hard-to-understand scientific background. To attract students and become a fully accepted learning material these videos need to be of a certain standard. Based upon our 8 years of experience it is important is to involve students directly into the concept and making-of (peer-to-peer approach), because students` needs and their perspectives on teaching material are directly included in …

The Colonisation Of The Tyrrhenian Islands By Hydraena Water Beetles, With Hydraena Reflexa Rey, 1884 Reinstated As A Valid Species Endemic To Corsica And Sardinia (Coleoptera, Hydraenidae), David T. Bilton, Ignacio Ribera, Adrián Villastrigo

School of Biological and Marine Sciences

The biotas of old islands formed from continental terranes usually have a more complex biogeographical history than those of young oceanic islands, including taxa which have originated by vicariance and/or colonisation, over a variety of timescales. The Tyrrhenian Islands of Corsica, Sardinia and the Tuscan Archipelago in the Mediterranean have a complex geological history, shaped by interac-tions between the African and Eurasian plates since the Mesozoic. Our understanding of the historical biogeography of Tyrrhenian endemics remains limited for many groups, including freshwater invertebrates. Here we use a time-calibrated phylogeny, derived from mitochondrial and nuclear DNA sequence data, to explore the …

October 10, 2023 Ereporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Economic Equity And People With Disabilities: Development And Characterization Of A Novel Index, Bhavneet Walia, Katherine Mcdonald, Barry Whaley, Vinh Nguyen

Burton Blatt Institute

Here, we develop two new social indices: the ADA PARC Absolute Economic Opportunity Index and the ADA PARC Relative Economic Opportunity Index. These indices allow us novel examinations of economic equity between people with and without disabilities within a U.S. state and between people with disabilities in different states using aggregations of multiple component economic indicators. These represent the first efforts to offer U.S. indices of this focus, an important development given the distinct economic needs of people with disabilities and the value in accounting for distinct national policies. The indices rely on U.S. Census and other data on economic …

Antithrombotic Nanoparticles And Methods For Treating Necrotizing Enterocolitis, Akhil Maheshwari, Samuel Wickline, Kopperuncholan Namachivayam, Hua Pan

USF Patents

The present disclosure is directed to antithrombotic nanoparticles and methods of treating necrotizing enterocolitis.

Inducing Mitotic Catastrophe As A Therapeutic Approach To Improve Outcomes In Ewing Sarcoma., Soumya M. Turaga, Vikalp Vishwakarma, Stacey L. Hembruff, Benjamin K. Gibbs, Priya Sabu, Rajni V. Puri, Harsh B. Pathak, Glenson Samuel, Andrew K. Godwin

Manuscripts, Articles, Book Chapters and Other Papers

Ewing sarcoma (EWS) is an aggressive pediatric malignancy of the bone and soft tissues in need of novel therapeutic options. To identify potential therapeutic targets, we focused on essential biological pathways that are upregulated by EWS-FLI1, the primary oncogenic driver of EWS, including mitotic proteins such as Aurora kinase A (AURKA) and kinesin family member 15 (KIF15) and its binding partner, targeting protein for Xklp2 (TPX2). KIF15/TPX2 cooperates with KIF11, a key mitotic kinesin essential for mitotic spindle orientation. Given the lack of clinical-grade KIF15/TPX2 inhibitors, we chose to target KIF11 (using SB-743921) in combination with AURKA (using VIC-1911) given …

Phase Shift Between Joint Rotation And Actuation Reflects Dominant Forces And Predicts Muscle Activation Patterns, R. D. Quinn, Hillel J. Chiel

Faculty Scholarship

During behavior, the work done by actuators on the body can be resisted by the body's inertia, elastic forces, gravity, or viscosity. The dominant forces that resist actuation have major consequences on the control of that behavior. In the literature, features and actuation of locomotion, for example, have been successfully predicted by nondimensional numbers (e.g. Froude number and Reynolds number) that generally express the ratio between two of these forces (gravitational, inertial, elastic, and viscous). However, animals of different sizes or motions at different speeds may not share the same dominant forces within a behavior, making ratios of just two …

Leveraging The Strengths Of Citizen Science And Structured Surveys To Achieve Scalable Inference On Population Size, Andrew N. Stillman, Paige E. Howell, Guthrie S. Zimmerman, Emily R. Bjerre, Brian A. Millsap, Orin J. Robinson, Daniel Fink, Erica F. Stuber, Viviana Ruiz-Gutierrez

Wildland Resources Faculty Publications

1. Population size is a key metric for management and policy decisions, yet wildlife monitoring programmes are often limited by the spatial and temporal scope of surveys. In these cases, citizen science data may provide complementary information at higher resolution and greater extent.

2. We present a case study demonstrating how data from the eBird citizen science programme can be combined with regional monitoring efforts by the US Fish and Wildlife Service to produce high-resolution estimates of golden eagle abundance. We developed a model that uses aerial survey data from the western United States to calibrate high-resolution annual estimates of …

Dc Grid Power Congestion Management Laboratory Experiments, Holly Engelbrecht, Diego Zuidervliet, Peter Van Duijsen

Research Papers

In the process of the electrical energy transition, a new curriculum for bachelor electrical engineering is developed. A new development is DC grids, as they are shown to be promising in solving the power congestion management problem. Particularly when adding solar power, battery storage, and load appliances including power electronics, DC grids are replacing AC grids, especially in micro-grids. The development of new laboratory experiments using three educational methods is described in this paper. First, theory combined with online calculation tools is used to prepare the students for the subject. Second, the experiment has to be prepared using simulation tools, …

Professional Identity Of Female Engineering Graduates: An Exploration Of Identity Status Through Life History Research, Natascha Van Hattum-Janssen, Maaike D. Endedijk

Research Papers

The number of students entering engineering programmes is too low to meet the need for engineering graduates. Still, many leave for jobs outside the technical sector right after graduation. Professional identity is a concept that helps to explain why they stay in or leave the technical sector (Cech 2014). It is the result of the process of professional socialisation. This study uses life history research to understand the professional socialisation of engineering graduates from kindergarten age until a few years after graduation. An analysis of the life experiences of male and female engineering graduates shows differences in how they describe …

Fam210a Is Essential For Cold-Induced Mitochondrial Remodeling In Brown Adipocytes, Jiamin Qiu, Feng Yue, Peipei Zhu, Jingjuan Chen, Fan Xu, Lijia Zhang, Kun Ho Kim, Madigan M. Snyder, Nanjian Luo, Hao-Wei Xu, Fang Huang, W. Andy Tao, Shihuan Kuang

Purdue University Libraries Open Access Publishing Fund

Cold stimulation dynamically remodels mitochondria in brown adipose tissue (BAT) to facilitate non-shivering thermogenesis in mammals, but what regulates mitochondrial plasticity is poorly understood. Comparing mitochondrial proteomes in response to cold revealed FAM210A as a cold-inducible mitochondrial inner membrane protein. An adipocyte-specific constitutive knockout of Fam210a (Fam210aAKO) disrupts mitochondrial cristae structure and diminishes the thermogenic activity of BAT, rendering the Fam210aAKO mice vulnerable to lethal hypothermia under acute cold exposure. Induced knockout of Fam210a in adult adipocytes (Fam210aiAKO) does not affect steady-state mitochondrial structure under thermoneutrality, but impairs cold-induced mitochondrial remodeling, leading to progressive loss of cristae and reduction of …

State Of Utah, Plaintiff/Appellee V. Debra Kay Youren, Defendant/Appellant : Brief Of Appellant, Utah Court Of Appeals

Utah Court of Appeals Briefs (2020 –)

Appeal from a Judgment and Conviction for one count of Exploitation of a Vulnerable Adult, a Second Degree Felony, and one count of Theft, a Second Degree Felony, The Honorable Anthony Howell presiding in the Fourth Judicial Court, Juab County, State of Utah, Case No.191600058

SEAN REYES Utah Attorney General c/o Criminal Appeals Division Assistant Solicitor General Attorney for Appellee

ANN M. TALIAFERRO (#8776) BROWN BRADSHAW & MOFFAT Attorney for Appellant

Novel Treatments For Pxe: Targeting The Systemic And Local Drivers Of Ectopic Calcification, Ida Joely Jacobs, Qiaoli Li

Department of Biochemistry and Molecular Biology Faculty Papers

Pseudoxanthoma elasticum (PXE) is a heritable multisystem ectopic calcification disorder. The gene responsible for PXE, ABCC6, encodes ABCC6, a hepatic efflux transporter regulating extracellular inorganic pyrophosphate (PPi), a potent endogenous calcification inhibitor. Recent studies demonstrated that in addition to the deficiency of plasma PPi, the activated DDR/PARP signaling in calcified tissues provides an additional possible mechanism of ectopic calcification in PXE. This study examined the effects of etidronate (ETD), a stable PPi analog, and its combination with minocycline (Mino), a potent inhibitor of DDR/PARP, on ectopic calcification in an Abcc6-/- mouse model of PXE. Abcc6-/- mice, at 4 weeks of …

Phytochemical Constituent And Antioxidant Activity Evaluation Of Red Seaweed Eucheuma Sp., Ade Arsianti

Indonesian Journal of Medical Chemistry and Bioinformatics

Oxidative stress is a condition in which there is an imbalance between production of free radicals and protective response via antioxidant system. There are endogenous and exogenous antioxidants, however as age increases, there is a reduction in endogenous antioxidant, thus the search for potential exogenous antioxidants which could be derived from natural resources are needed. Indonesia is a megabiodiversity country which has more than 30,000 species of plants and animals. Red seaweed Eucheuma sp. is one of marine macroalgae species which shows potent biological activities. This study aims to determine the phytochemical constituent and to evaluate the antioxidant activity of …

Paired Liver:Plasma Pfas Concentration Ratios From Adolescents In The Teen-Labs Study And Derivation Of Empirical And Mass Balance Models To Predict And Explain Liver Pfas Accumulationn, Brittney O Baumert, Fabian C Fischer, Flemming Nielsen, Philippe Grandjean, Scott Bartell, Nikos Stratakis, Douglas I Walker, Damaskini Valvi, Rohit Kohli, Thomas Inge, Justin Ryder, Todd Jenkins, Stephanie Sisley, Stavra Xanthakos, Sarah Rock, Michele A La Merrill, David Conti, Rob Mcconnell, Lida Chatzi

Faculty, Staff and Students Publications

Animal studies have pointed at the liver as a hotspot for per- and polyfluoroalkyl substances (PFAS) accumulation and toxicity, however, these findings have not been replicated in human populations. We measured concentrations of seven PFAS in matched liver and plasma samples collected at the time of bariatric surgery from 64 adolescents in the Teen-Longitudinal Assessment of Bariatric Surgery (Teen-LABS) study. Liver:plasma concentrations ratios were perfectly explained (r2 > 0.99) in a multi-linear regression (MLR) model based on toxicokinetic (TK) descriptors consisting of binding to tissue constituents and membrane permeabilities. Of the seven matched plasma and liver PFAS concentrations compared in this …

The George-Anne Inkwell Edition, Georgia Southern University

The Inkwell

No abstract provided.

Cd13 Facilitates Immune Cell Migration And Aggravates Acute Injury But Promotes Chronic Post-Stroke Recovery, Justin N Nguyen, Eric C Mohan, Gargee Pandya, Uzma Ali, Chunfeng Tan, Julia K Kofler, Linda Shapiro, Sean P Marrelli, Anjali Chauhan

Faculty, Staff and Student Publications

Introduction

Acute stroke leads to the activation of myeloid cells. These cells express adhesion molecules and transmigrate to the brain, thereby aggravating injury. Chronically after stroke, repair processes, including angiogenesis, are activated and enhance post-stroke recovery. Activated myeloid cells express CD13, which facilitates their migration into the site of injury. However, angiogenic blood vessels which play a role in recovery also express CD13. Overall, the specific contribution of CD13 to acute and chronic stroke outcomes is unknown.

Methods

CD13 expression was estimated in both mice and humans after the ischemic stroke. Young (8–12 weeks) male wild-type and global CD13 knockout …

High Throughput Laser Ablation Icp-Ms Bioimaging Of Silver Distribution In Animal Organisms And Plant Tissue After Exposure To Silver Sulfide Nanoparticles, G Marolt, S Novak, Aj Kokalj, I Talaber, V Kononenko, S Loureiro, Z Khodaparast, Pv Silva, Mb Fité, Rd Handy, D Drobne

School of Biological and Marine Sciences

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a method with high potential to visualize the distribution of elements in different samples, including a variety of organisms. This study aimed to demonstrate the broad application of LA-ICP-MS in nanomaterial-biota fate studies as a high throughput bioimaging tool and to broaden the choice of standard organism models in material-biota interaction research. In this study, operation parameters of LA-ICP-MS were optimised on one organism, woodlice Porcellio scaber. Subsequently, the scanning conditions were tested on a range of aquatic (Girardia tigrina, Lumbriculus variegatus, and Oncorhynchus mykiss), terrestrial organisms (Lumbricus rubellus, Porcellio scaber) …

Revolutionising Engineering Education: Creating Photorealistic Virtual Human Lecturers Using Artificial Intelligence And Computer Generated Images, Johannes H Moolman, Fiona Boyle, Joseph Walsh

Research Papers

The COVID-19 pandemic has disrupted traditional classroom learning, making virtual and remote education increasingly important. In this context, the use of photorealistic virtual humans, or avatars, powered by Artificial Intelligence (AI) can offer an immersive and engaging environment for delivering traditional classroom-based lectures. This paper proposes a process that combines AI and Computer-Generated Images (CGI) to create photorealistic virtual human lecturers for educational purposes.

The proposed process flow involves generating audio from text inputs, which is passed to a 3-Dimensional (3D) facial animation rig that matches lip, tongue, eye and facial movements to the audio using AI. This generates a …

Addressing Long-Term Challenges In Energy For Sustainable Futures By Applying Moonshot Thinking, Andreas Sumper, Marc Jené-Vinuesa, Carlos González-De-Miguel, Maria Marin-Macaya

Research Papers

The rapid and exponential changes in our world require the education of engineers who can develop solutions to future and long-term challenges such as climate change. Exploration and innovation methodologies such as Futures Thinking and Moonshot Thinking have the potential to equip engineering students with useful tools and skills to build sustainable futures. To this end, the InnoEnergy MSc Energy for Smart Cities programme at BarcelonaTech (UPC) has developed a challenge-based learning (CBL) course that applies moonshot thinking to tackle major energy problems. This paper presents the methodology refined over three years of implementing the CBL course with second-year Masters's …

Exploration Of Corona Charge As A Novel In Vitro Human T Cell Dna Transfection Method, Molly A. Skinner

USF Tampa Graduate Theses and Dissertations

With gene-based immunotherapies on the rise to treat a multitude of diseases, the ability to genetically modify cells in a rapid and efficient fashion is necessary to keep up with demand. Gene electrotransfer (gene transfer by electroporation) is an appealing transfection method due to its low cost, high efficiency compared to other physical forms of gene delivery, and low toxicity compared to liposomal or viral vectors.

Traditional gene delivery via electroporation is done within a cuvette where the cell suspension is placed between two plate electrodes. This method requires cells to be moved from the treatment cuvette to a growth …

Dendritic Cells As Shepherds Of T Cell Immunity In Cancer, Mikael J Pittet, Mauro Di Pilato, Christopher Garris, Thorsten R Mempel

Faculty, Staff and Student Publications

In cancer patients, dendritic cells (DCs) in tumor-draining lymph nodes can present antigens to naive T cells in ways that break immunological tolerance. The clonally expanded progeny of primed T cells are further regulated by DCs at tumor sites. Intratumoral DCs can both provide survival signals to and drive effector differentiation of incoming T cells, thereby locally enhancing antitumor immunity; however, the paucity of intratumoral DCs or their expression of immunoregulatory molecules often limits antitumor T cell responses. Here, we review the current understanding of DC-T cell interactions at both priming and effector sites of immune responses. We place emerging …