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Nuclear Argonaute Hrde-1 Is Required For Proper Development And Maturation In C. Elegans, Hadeel Ali Zourkout

2023 Fall Honors Capstone Projects - Archive

Small interfering RNAs (siRNAs) maintain transgenerational gene silencing in C. elegans. siRNAs are loaded into Argonaute proteins to form an RNA-induced silencing complex (RISC), which functions in the nucleus or cytoplasm. In the nucleus, RISC directs the establishment of H3K9 trimethylation (H3K9me3) to silence loci. One nuclear Argonaute protein is HRDE-1. Here, we show that HRDE-1 is required for development and maturation in C. elegans. Brood size assays of hrde-1 mutants and wild-type (N2) hermaphrodites grown at permissive temperature (20℃) and under heat stress conditions (25℃) were performed to examine the number of eggs laid, eggs hatched, and matured progeny. …

Development Of S.T.E.A.M. Daises In Formal And Informal Environments For Educating Primary I Learners, Frances Neville

Masters Theses

The federal government has made education a competition among ourselves. Learning is not a rivalry to be imposed on our physical and cognitive development as humans. Escalating beliefs recognize education is more encompassing than CCSS, assessments and the grade level time frames in which standards are to be taught. On the precipice of anti-intellect, low expectations, and what has been termed “The Dumbing of America” an investigation into what it would take to close the achievement gap and increase student learning is overdue.

The goal for my Michigan based project, S.T.E.A.M. Daises, was to develop a program focusing on the …

Growing Agriculture Literacy’S Presence In America’S Classrooms, Emily Stone

Journal of Food Law & Policy

“Americans, as a whole, were at least two generations removed from the farm and did not understand even the most rudimentary of processes, challenges, and risks that farmers and the agricultural industry worked with and met head-on every day.” This quote perfectly describes the mindset of agriculture stakeholders in 1981 as they began to realize the drastic steps our education system had taken away from using principles of agriculture in K-12 education. As they saw it, Americans were moving out of rural America, away from farms, and becoming less connected to the food they daily consumed. Simultaneously, the education system …

The Contradictory Nature Of U.S. Laws And Nutrition Programs And Their Effects On Infant Feeding, Lily Patel

Journal of Food Law & Policy

The contradictory nature of U.S. laws, including the laws concerning infant feeding, though supposedly aligned with policies to promote wellness in Americans, can exacerbate gender and race inequality and work against the National Strategy. The overarching goal of U.S. laws concerning infant feeding is to ensure that infants are fed, nourished, and receive proper nutrition. However, the laws often appear to be directly contradictory to one another in the priorities they are promoting.

Chewing The Welfare Cud: A Digested Analysis Of A Consumer Versus Producer-Defined Standard Of Welfare Practices In Animals Raised For Human Consumption, Caitlin C. Robb

Journal of Food Law & Policy

Since the eighteenth century, animal well-being remains a concern for American citizens. Yet, underlying this concern is the thought that while humans should not be cruel to animals, animals are still private property subject to human ownership. Therefore, multi-faceted questions of what constitutes “animal welfare” find a place in modern American debate. One such question becomes: should the producer or the consumer define welfare practice standards of animals raised for human consumption?7 This note provides an answer to this question by first analyzing the robust history of animal welfare in the United States, along with the domestic and international impact …

Racial Dis/Embodiment: A Discourse Theoretical Analysis Of University International Offices’ Websites, Gian-Louis Hernandez

Journal of Comparative & International Higher Education

Special Issue

Ethanol Dysregulates Sirt1-Mediated Lipogenic Signaling Pathways Through Α4*-Nicotinic Acetylcholine Receptors In Hepatocytes., Caitlin Christina Wilkerson

Electronic Theses and Dissertations

Alcohol-associated liver disease (ALD) is a broad spectrum of diseases ranging from steatosis to severe hepatic cirrhosis, none of which have any FDA approved therapies. Downregulation of hepatic Sirtuin 1 (Sirt1) activity by ethanol has been shown to lead to an upregulation of the pro-lipogenic gene targets under its jurisdiction, resulting in increased lipogenesis and triglyceride accumulation. It was hypothesized that ethanol acts on α4*-nicotinic acetylcholine receptors (α4*-nAChRs) in hepatocytes to decrease Sirt1 expression, which ultimately dysregulates lipid metabolic homeostasis. Ethanol’s effects were investigated in cultured AML12 hepatocytes and in WT primary mouse hepatocytes acutely exposed to ethanol. Consistent with …

Environmental Exposures And Aging., Daniel Chris Gomes

Electronic Theses and Dissertations

In recent years, research into air pollution has shown that exposure to certain components in air pollution, primarily PM2.5 can accelerate biological aging and thereby lead to increased susceptibility to multiple diseases. We hypothesize that prolonged exposure to air pollutants can result in premature aging leading to extensive tissue dysfunction and susceptibility to diseases. To examine this, we exposed mice to PM2.5 for 9, 15, and 21 days, then measured the telomere lengths, cellular senescence, and histone methylation patterns of multiple cell types. We found consistently increased telomere attrition, cellular senescence and advanced age-consistent histone methylation patterns in groups exposed …

Study Of The Effects Of Chronic Cadmium Exposure On The Pathogenesis Of Pulmonary Arterial Hypertension., Dakotah Dominique Cathey

Electronic Theses and Dissertations

We reported pulmonary artery hypertension (PAH) patients had elevated levels of Cd in both blood and urine, therefore, this study tested whether Cd directly induces PAH or facilitates PAH pathogenesis in mouse models. C57/6J mice were initially exposed to drinking water with or without 5ppm Cd for 8 weeks. Then, half the mice in both control and Cd groups were given SU5416 and hypoxia (SuHx) for 4 weeks to induce PAH, resulting in 4 subgroups: Control, Cd, PAH, and Cd+PAH. Diastolic and systolic functions of the left and right ventricles (LV, RV) were examined with echocardiography before and after PAH. …

Genetically Engineering Gingipains For High Yield Large Scale Purification., Andrew E. Fuchs

Electronic Theses and Dissertations

Porphyromonas gingivalis is a keystone oral pathogen in the progression of periodontal disease and has been implicated in the etiology of many other systemic human diseases. P. gingivalis produces primary virulence factors termed the gingipains, a group of cysteine proteases. There is a high demand for high purity gingipains for downstream in vitro studies where P.g. is implicated, both oral and systemic. Gingipain production candidate mutant P.g. strains were generated to isolate each gingipain for large scale purification and the purified gingipains characterized. Two mutants were determined not to be viable for large scale purification of gingipains, each for different …

The Interplay Between Lung Adenocarcinoma, Nutrient Availability, And The Microbiome., Alexis A. Vega

Electronic Theses and Dissertations

Lung cancer is one of the most common forms of cancer diagnosed, and has the highest lethality rate across all other forms of cancer in the U.S. While current therapeutic options include chemo-, immuno-, and radio-therapy, the benefits of caloric or nutrient restriction on cancer cells has also been investigated. Due to the many biological functions associated with methionine, many have proposed a methionine restricted diet would lead to favorable outcomes when combating cancer. Although our cells are incapable of synthesizing methionine, the bacteria found in our microbiome can. Furthermore, recent discoveries suggest an independent microbiome found within tumors that …

Investigating The Effects Of Subchronic Dietary Zinc Supplementation On Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease., Oluwanifemi Esther Bolatimi

Electronic Theses and Dissertations

Zinc deficiency is associated with metabolic dysfunction-associated steatotic liver disease (MASLD). Previous studies show zinc supplementation improves steatosis, but its therapeutic effects in established MASLD remain unclear. We developed a model to characterize the effects of zinc supplementation on high-fat diet (HFD) induced MASLD and hypothesized established MASLD would be attenuated. Mice were fed control diet or HFD for 12 weeks and then grouped into normal or zinc-supplemented diets for 8 additional weeks. At euthanasia, plasma and liver tissues were collected for phenotypic analysis. Twelve weeks of HFD altered glucose clearance and body composition. Eight weeks of subsequent zinc supplementation …

The Impact Of Environmental Pollutants On Alcohol-Associated Liver Disease Pathogenesis., Tyler Charles Gripshover

Electronic Theses and Dissertations

Steatotic liver disease is brought upon by differing etiologies and its global prevalence is expected to keep increasing over the next several decades. Particularly, alcohol-associated liver disease (ALD) manifests from excessive alcohol consumption where the liver retains lipids and progresses with metabolic dysfunction. Previous studies have demonstrated that environmental pollutants, polychlorinated biphenyls (PCBs) promote metabolic dysfunction associated steatotic liver disease (MASLD), in diet-induced obesity models. However, it is unknown if environmental pollutants may also promote ALD pathogenesis. Thus, this dissertation focuses on characterizing the effects of PCB126 in a rodent ALD model followed by multiple ‘Omics approaches to identify mechanisms …

Environmental Exposures And Male Reproductive Outcomes., Damilola Rukayat Owoade

Electronic Theses and Dissertations

Male sexual dysfunction comprises various conditions, including erectile dysfunction (ED) and ejaculatory dysfunction. Testosterone deficiency (TD), which has been linked to some of these sexual dysfunctions, is characterized by low production of testosterone by the testes. These male reproductive outcomes may develop due to exposure to various risk factors, including environmental exposures. These environmental exposures may include volatile organic compounds (VOCs) and tear gas. This dissertation aims to examine the effects of tear gas exposure and VOCs on male reproductive outcomes. The association between tear gas exposure and male reproductive outcomes was assessed using data obtained from an online anonymous …

Automated Usability Evaluation Utilizing Log Files And Data Mining Techniques., Sima Shafaei

Electronic Theses and Dissertations

Usability evaluation is one of the essential aspects of software production. This evaluation should be done during the entire life cycle of a software application, from pre-production to production and post-production. However, the collection and evaluation of usability data can be a very challenging, time-consuming, and expensive task to be conducted manually, particularly for certain types of products and working conditions. These challenges may include the need to recruit participants fully engage and motivate them during evaluation, and factor in environmental conditions. Other challenges may include collecting data in real-world environments, especially when the users are geographically dispersed, minimizing evaluator …

Effects Of A Protection Zone In A Reaction-Diffusion Model With Strong Allee Effect., Isaac Johnson

Electronic Theses and Dissertations

A protection zone model represents a patchy environment with positive growth over the protection zone and strong Allee effect growth outside the protection zone. Generally, these models are considered through the corresponding eigenvalue problem, but that has certain limitations. In this thesis, a general protection zone model is considered. This model makes no assumption on the direction of the traveling wave solution over the Strong Allee effect patch. We use phase portrait analysis of this protection zone model to draw conclusions about the existence of equilibrium solutions. We establish the existence of three types of equilibrium solutions and the necessary …

Design, Fabrication, And Integration Of Robotic Skin Sensors For Human Robot Interaction., Olalekan Olakitan Olowo

Electronic Theses and Dissertations

Enhancing physical human-robot interaction in modern robotics relies on refining the tactile perception of robot skin sensors. This research focuses on crucial aspects of the development process, including fabrication techniques, miniaturization, and integration for a more efficient collaborative human-robot interface. The fabrication process of robot skin sensors, designed to mimic human skin, is explored both within and outside cleanroom environments. An enhanced technique is presented to increase fabrication yield and create more miniaturized sensor designs with feature sizes in the tens of microns. These sensors function as piezoresistive arrays using organic polymers like PEDOT: PSS as the pressure-sensing medium. Various …

Impacts Of Environmental Stressors On Native South Dakota Amphibian Physiology And Survival, Danielle Jean Galvin

Dissertations and Theses

Amphibian populations around the world are declining, with some of the most likely drivers behind these declines including emerging infectious diseases and environmental contaminants. To address major gaps in the current literature, I sought to evaluate the effect of two major environmental stressors on various aspects of amphibian physiology: emerging infectious diseases and environmental contaminants. Emerging infectious diseases of amphibians include fungal, viral, and parasitic pathogens which have expanded in host range, either geographically or in competent host species. Environmental contaminants include chemicals which may be naturally occurring in the environment, or which may be introduced to the environment, often …

A Comparison Of Devices For Race Day Characterization Of North American Turfgrass Thoroughbred Racing Surfaces, Peter Schmitt, Wayne T. Sanderson, John Trey Rogers Iii, Tyler J. Barzee, Michael L. Peterson

UK CARES Faculty Publications

Both pre-race meet and daily turf surface condition measurements are required by regula- tions adopted as part of the Horseracing Integrity and Safety Act (HISA). The Orono Biomechanical Surface Tester (OBST) is the primary device used for characterizing a racing surface and is used for the pre-meet inspections. Tools that are better suited for the daily testing of turf surfaces are also needed to meet the new federal regulations. The purpose of this study was to compare five simple tools commonly used in turf applications to the OBST. Data were collected with each of the six devices at plots chosen …

Aging And Urinary Control: Alterations In The Brain-Bladder Axis., Cara C Hardy, Ron Korstanje

Faculty Research 2023

Age-associated alterations in bladder control affect millions of older adults, with a heavy burden added to families both economically and in quality of life. Therapeutic options are limited with poor efficacy in older adults, lending to a growing need to address the gaps in our current understanding of urinary tract aging. This review summarizes the current knowledge of age-associated alterations in the structure and function of the brain-bladder axis and identifies important gaps in the field that have yet to be addressed. Urinary aging is associated with decreased tissue responsiveness, decreased control over the voiding reflex, signaling dysfunction along the …

Clinical Practice Skills For Incorporating Animal-Assisted Therapy In Occupational Therapy, Erin Wilhelm

Occupational Therapy Doctoral Capstones

The aim of my capstone project was to advance the field of animal-assisted therapy (AAT) and promote the incorporation of AAT within occupational therapy (OT) services. Existing research notably supports the implementation of AAT in OT practice in many settings and for a wide variety of diagnoses to promote occupational performance, build therapeutic rapport, and encourage motivation and engagement in therapy, but stronger evidence is needed to promote the incorporation of AAT (Andreason et al., 2017; Fine et al., 2019b). Despite the growth of AAT over the past two decades, there are few resources tailored specifically to the implementation of …

Disinfection Of Foodborne Bacteria Using The Contamination Sanitization Inspection And Disinfection (Csi-D) Device, Jennifer Mccoy Sanders

Food Science (MS) Theses

Foodborne pathogens, including Escherichia coli, Salmonella enterica, and Listeria monocytogenes, are major causes of gastrointestinal disease globally. The Contamination Sanitization Inspection and Disinfection (CSI-D) device is a new handheld fluorescence-based imaging system designed to disinfect food contact surfaces contaminated with microorganisms using ultraviolet-C (UVC) illumination. This study aimed to determine the optimal parameters for the disinfection of foodborne bacteria using the CSI-D device. The following bacterial strains were tested: generic E. coli; enterohemorrhagic E. coli (EHEC) O157:H7; enterotoxigenic E. coli (ETEC) O78:H11; S. enterica serotypes Enteritidis, Newport, Typhimurium, and Javiana; and L. monocytogenes serotypes 1/2a, 1/2b, and 4b …

Salivary Duct Carcinoma Arising In A Warthin Tumor Of The Parotid Gland: A Rare Case Report With Review Of Literature And Pd-L1 Expression, Kaitlyn J. Nielson, Gamaliel Lorenzo, Shweta Agarwal

Pathology Research and Scholarship

Warthin's tumor is the second most common neoplasm of the parotid gland and consists of 2 components, including lymphoid stroma and glandular epithelium. Malignant transformation in this tumor is mostly seen in the lymphoid component; however, the carcinomatous transformation of the epithelial component is extremely rare. Cases of latter reported in the literature include squamous cell carcinoma, adenocarcinoma, mucoepidermoid carcinoma, oncocytic carcinoma, Merkel cell carcinoma, and undifferentiated carcinoma. We describe an extremely rare case of salivary duct carcinoma arising in a Warthin tumor in a 64-year-old male. Patient presented with an enlarging left parotid mass, biopsy of which showed salivary …

Iron Promotes Glycolysis To Drive Colon Tumorigenesis., Zhaoli Liu, Luke Villareal, Lavanya Goodla, Hyeoncheol Kim, Daniel M. Falcon, Mohammad Haneef, David R. Martin, Li Zhang, Ho-Joon Lee, Daniel Kremer, Costas A. Lyssiotis, Yatrik M. Shah, Henry C. Lin, Hui-Kuan Lin, Xiang Xue

Pathology Research and Scholarship

Colorectal cancer (CRC) is the third most common cancer and is also the third leading cause of cancer-related death in the USA. Understanding the mechanisms of growth and progression of CRC is essential to improve treatment. Macronutrients such as glucose are energy source for a cell. Many tumor cells exhibit increased aerobic glycolysis. Increased tissue micronutrient iron levels in both mice and humans are also associated with increased colon tumorigenesis. However, if iron drives colon carcinogenesis via affecting glucose metabolism is still not clear. Here we found the intracellular glucose levels in tumor colonoids were significantly increased after iron treatment.

Fall Field Report, August–November 2023, W. Ross Silcock

Nebraska Bird Review

As in the last few fall seasons, late departure dates continue to increase in number. This is often attributed to a warming climate but may also result from increased observer activity and usage of eBird. Late dates are probably the most important aspect of this report, most prominent the record late fall dates for Ruby-throated Hummingbird, Sanderling, Bank Swallow, and Cassin’s Sparrow. Although these species are taxonomically diverse, there were species group concentrations of late dates, notably among shorebirds. Examples were Marbled Godwit for which the four latest reports this fall were four of the latest six fall dates on …

Index To Volume 91

Nebraska Bird Review

Adams: Pam 11; Paul 11
Aden, Debbie 55
Allard, Savannah 29
Allen, Edward 10
Allison, Betty 30
Alpheus, A. 154
Altman, Dakota 21, 30
Andersen: Bennett 30; Brett 30, 52, 142; D. E. 83
Anderson: Bob 139, 147; Chris 7; Janet 29; Will 68
Angkaew, Rongron 6
Ani, Groove-billed 127, 131
Anich: Charlie 99; Nick 99; Paula 99
Arends, Jim 30
Armstrong, Wyatt 121
Askerooth, Kristine 29
Atkins, Kate 16
Atwell, Erik 55, 58, 99, 100, 102, 112
Audubon, M. 82
Augustine, Jackie 63
Austin, Mary 7
Avocet, American 19, 53, 86, 100, 132, 158

. . .

Zach, Joel …

Yellow-Throated Warbler In Scotts Bluff County, Nebraska, Mark A. Brogie, Bill F. Huser, Jerry Probst

Nebraska Bird Review

Yellow-throated Warbler (Setophaga dominica) is a rare to uncommon migrant in eastern and central Nebraska. It is possibly a regular breeder locally at sites containing sycamores in several southeastern Nebraska counties bordering the Missouri River. There are few Nebraska reports after July, with more reports November–January than August–October. This species has three previous documented Nebraska Panhandle records, all from May.

1. One photographed 26 May 2009, at Crescent Lake NWR, Garden Co.

2. One seen and “well described” 06 May 1931, at Antioch, Sheridan Co.

3. A specimen taken 20 May 1917, at Oshkosh, Garden Co., was once …

Defensive Behaviors Of The Mealybug Nipaecoccus Nipae (Maskell, 1893) (Hemiptera: Pseudococcidae) And The Green Lacewing Ceraeochrysa Claveri (Navás, 1911) (Neuroptera: Chrysopidae), With Videos Of Dorsal Packet Loading And Mealybug Ostiole Function, Erin C. Powell

Insecta Mundi

The interactions between the lacewing Ceraeochrysa claveri (Navás) (Neuroptera: Chrysopidae) larva and the coconut mealybug Nipaecoccus nipae (Maskell) (Hemiptera: Pseudococcidae) were recorded. The third-instar lacewing larva constructed a dorsal packet using mealybug wax from both male pupal cases and the bodies of adult females. Surprisingly, live nymphs were also frequently placed into the dorsal packet. Prey mealybugs were discarded and not incorporated into the packet after consumption. When disturbed, adult female mealybugs reflex bled from their dorsal ostioles, contacting the mouthparts of the lacewing. The lacewing quickly retreated to clean the mouthparts on the substrate, providing further evidence that ostiolar …

Predicting The Potential Distribution In South Korea Of Two Mealybug Species (Hemiptera: Pseudococcidae) Intercepted On Pineapples In Quarantine, S Bin Kim, Soo-Jung Suh

Insecta Mundi

Dysmicoccus brevipes (Cockerell) and Dysmicoccus neobrevipes Beardsley are major pests of pine­apples, ornamentals, and vegetable crops in many countries around the world. The potential distribution of these mealybug pests into South Korea remains a prime concern because of their high incidence in intercep­tions screened during inspection. Hence, these species prompted a modelling effort to assess their potential risk of introduction. Potential risk maps were developed for these pests with the CLIMEX model based on occurrence records under environmental data. The potential distribution of these pests in South Korea in the 2020s, 2050s and 2090s is projected based on the RCP …

The Nebraska Bird Review (December 2023) 91(4), Whole Issue

Nebraska Bird Review

Fall Field Report, August–November 2023, by W. Ross Silcock

NOU Founding Ornithologist, Hypnotist, Physician: Isador Trostler, by Rick Wright

Yellow-throated Warbler in Scotts Bluff County, Nebraska, by Mark A. Brogie, Bill F. Huser, and Jerry Probst

Holdrege Fall Field Days, Sept. 22–24, 2023

Index to Volume 91

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