Digital Commons Network^™

Lipid-Nanoparticle-Mediated Delivery Of Docetaxel Prodrug For Exploiting Full Potential Of Gold Nanoparticles In The Treatment Of Pancreatic Cancer, Abdulaziz Alhussan, Nolan Jackson, Sarah Eaton, Nancy Dos Santos, Ingrid Barta, Josh Zaifman, Sam Chen, Yuen Yi C Tam, Sunil Krishnan, Devika B Chithrani

Faculty, Staff and Student Publications

Current chemoradiation therapy suffers from normal tissue toxicity. Thus, we are proposing incorporating gold nanoparticles (GNPs) and docetaxel (DTX), as they have shown very promising synergetic radiosensitization effects. Here, we explored the effect of a DTX prodrug encapsulated in lipid nanoparticles (LNP_DTX-P) on GNP uptake in pancreatic cancer models in vitro and in vivo. For the in vitro experiment, a pancreatic cancer cell line, MIA PaCa-2, was cultured and dosed with 1 nM GNPs and 45 nM free DTX or an equivalent dose of LNP_DTX-P. For the in vivo experiment, MIA PaCa-2 cells were implanted subcutaneously …

Effect Of Dietary Protein On Honey Bee Pollen Foraging Behavior, Katherine Burke

Biology Student Scholarship

Bees obtain nutrients from flowers. Pollen provides proteins and fats; nectar provides carbohydrates. Pollen is important for providing essential amino acids which honey bees must obtain from their diet for proper development. Commercial honey bee colonies, however, pollinate monocultures, which contain one crop type thus, one unbalanced nutritional resource. We examined how a lack of protein diversity affects honey bee pollen foraging behavior. Bees were raised on three diet treatments: no manipulation, all 10 essential amino acids (EAAs, i.e., protein building-blocks), or only 6/10 EAAs. Bee-collected pollen was trapped upon return to the hive and nutritional content was analyzed. We …

Effect Of Decreased Snowpack On A Rare Butterfly’S Host Plant, Caitlin Mchugh

Biology Student Scholarship

Earth’s increased global temperature as a response to climate change has caused less snowfall and earlier snowmelt in spring. These changes can alter the phenology of organisms such as through plant leafing and flower blooming. This can be harmful to species reliant on plants that are more sensitive to temperature changes. The frosted elfin (Callophrys irus) is a species of concern in 11 states—including RI, MA, and CT. These host plant specialists only lay eggs on small yellow wild indigo (Baptisia tinctoria) and wild lupine (Lupinus perennis). If neither plant emerges and develops leaves …

Tackling The Challenge, Keith Testa

UNH Today Archive

No abstract provided.

Editorial: The Biology And Management Of Chronic Diseases In Mexican Americans, Eron G. Manusov, Belinda M. Reininger, Alfredo Ulloa-Aguirre, John Blangero

School of Medicine Publications

No abstract provided.

Fracture Toughness Of Electrospun Fibrous Mats, Annabella Mascot

Mechanical Engineering and Materials Science Independent Study

Fiber reinforced composite hydrogels have become a popular material used in tissue engineering in recent years due to their robust nature under tension and biocompatibility qualities [1]. However, little is known of the fracture mechanics of the fiber reinforcements themselves (which are typically created of Electrospun materials, a process in which a high voltage differential is applied to a polymer solution, creating a jet of nanofibers that collect into a mat- like material) [2]. This study provides ana analysis of the fracture toughness of two Electrospun materials: one created from a 100 bloom strength gelatin solution, and one created from …

Pum1 Mediates The Posttranscriptional Regulation Of Human Fetal Hemoglobin, Yuncheng Man, Amma Owusu-Ansah, Omar Y. Mian, Umut A. Gurkan, Anton A. Komar, Merlin Nithya Gnanapragasam

Faculty Scholarship

The fetal-to-adult hemoglobin switching at about the time of birth involves a shift in expression from γ-globin to β-globin in erythroid cells. Effective re-expression of fetal γ-globin can ameliorate sickle cell anemia and β-thalassemia. Despite the physiological and clinical relevance of this switch, its posttranscriptional regulation is poorly understood. Here, we identify Pumilo 1 (PUM1), an RNA-binding protein with no previously reported functions in erythropoiesis, as a direct posttranscriptional regulator of β-globin switching. PUM1, whose expression is regulated by the erythroid master transcription factor erythroid Krüppel-like factor (EKLF/KLF1), peaks during erythroid differentiation, binds γ-globin messenger RNA (mRNA), and reduces γ-globin …

Biofilm Characterization And The Potential Role Of Edna In Horizontal Gene Transfer In Hospital And Meat Isolates Of Staphylococcus Aureus And Their Biofilms, Ashley Lynne Ball

Theses and Dissertations

Staphylococcus aureus is a pathogen responsible for a wide variety of life-threatening diseases such as bacteremia, endocarditis, and pneumoniae. S. aureus has been a major concern in recent years due to the rampant spread of antibiotic resistance. The ability of S. aureus to form biofilms aids in the spread of antibiotic resistance as biofilms are a known hotspot for horizontal gene transfer. Biofilms also protect cells from host immune responses and antibiotics, making these infections very difficult to treat. The matrix of S. aureus biofilms can be made of polysaccharides, protein, and DNA. In these studies, we sought to elucidate …

Computer Clinical Decision Support That Automates Personalized Clinical Care: A Challenging But Needed Healthcare Delivery Strategy, Alan H Morris, Christopher Horvat, Brian Stagg, David W Grainger, Michael Lanspa, James Orme, Terry P Clemmer, Lindell K Weaver, Frank O Thomas, Colin K Grissom, Ellie Hirshberg, Thomas D East, Carrie Jane Wallace, Michael P Young, Dean F Sittig, Mary Suchyta, James E Pearl, Antinio Pesenti, Michela Bombino, Eduardo Beck, Katherine A Sward, Charlene Weir, Shobha Phansalkar, Gordon R Bernard, B Taylor Thompson, Roy Brower, Jonathon Truwit, Jay Steingrub, R Duncan Hiten, Douglas F Willson, Jerry J Zimmerman, Vinay Nadkarni, Adrienne G Randolph, Martha A Q Curley, Christopher J L Newth, Jacques Lacroix, Michael S D Agus, Kang Hoe Lee, Bennett P Deboisblanc, Frederick Alan Moore, R Scott Evans, Dean K Sorenson, Anthony Wong, Michael V Boland, Willard H Dere, Alan Crandall, Julio Facelli, Stanley M Huff, Peter J Haug, Ulrike Pielmeier, Stephen E Rees, Dan S Karbing, Steen Andreassen, Eddy Fan, Roberta M Goldring, Kenneth I Berger, Beno W Oppenheimer, E Wesley Ely, Brian W Pickering, David A Schoenfeld, Irena Tocino, Russell S Gonnering, Peter J Pronovost, Lucy A Savitz, Didier Dreyfuss, Arthur S Slutsky, James D Crapo, Michael R Pinsky, Brent James, Donald M Berwick

Faculty, Staff and Student Publications

How to deliver best care in various clinical settings remains a vexing problem. All pertinent healthcare-related questions have not, cannot, and will not be addressable with costly time- and resource-consuming controlled clinical trials. At present, evidence-based guidelines can address only a small fraction of the types of care that clinicians deliver. Furthermore, underserved areas rarely can access state-of-the-art evidence-based guidelines in real-time, and often lack the wherewithal to implement advanced guidelines. Care providers in such settings frequently do not have sufficient training to undertake advanced guideline implementation. Nevertheless, in advanced modern healthcare delivery environments, use of eActions (validated clinical decision …

Hypermethylation Of Nc886 In Hpv-Positive Oropharyngeal Cancer And Its Clinical Implications: An Epigenome-Wide Association Study, Yifan Xu, Ziqiao Wang, Peng Wei, Richa Gairola, Karl T Kelsey, Andrew G Sikora, Guojun Li, Jian Gu

Faculty, Staff and Student Publications

The incidence of oropharyngeal squamous cell carcinoma (OPSCC) has increased rapidly in the United States, driven by rising human papillomavirus (HPV) infections in the U.S. population. HPV-positive OPSCC patients have a better prognosis than HPV-negative patients. To gain insights into the unique biology of HPV(+) OPSCC that may contribute to its clinical behaviors, we performed a multi-stage epigenome-wide methylation profiling of leukocyte and tumor DNA in OPSCC patients and compared the methylation levels of CpG sites between HPV(+) and HPV(-) OPSCC patients. We identified and validated a significantly differentially methylated region (DMR) of 1,355 bp encompassing non-coding RNA 886 ( …

Association Of Fabry Disease With Hearing Loss, Tinnitus, And Sudden Hearing Loss: A Nationwide Population-Based Study, Yen-Fu Cheng, Sudha Xirasagar Mbbs., Ph.D., Chin-Shyan Chen, Dau-Ming Niu, Herng-Ching Lin

Publications

Hearing loss and the related otologic manifestations are receiving increased scrutiny as significant causes of morbidity in Fabry disease. However, the relative risks of auditory deficits among patients with Fabry disease relative to the general population without a diagnosis of Fabry disease have not been studied. This study aims to explore the associations between Fabry disease and hearing-related manifestations using a nationwide population-based dataset. We identified study patients for this cross-sectional study from the 2015−2017 claims databases of the Taiwan Longitudinal Health Insurance Database 2005. We first identified 2312 patients aged over 20 years with a diagnosis of Fabry disease. …

Scientists And Activists Work To Save The Planet, Myriam G. Vidal Valero

Capstones

Climate change and human intervention in nature are affecting people, ecosystems and ways of living all over the world. This portfolio of environmental pieces showcases the dire consequences of not addressing these issues, how solutions can be reached and the challenges facing those who try to change things.

Gata1 Controls Numbers Of Hematopoietic Progenitors And Their Response To Autoimmune Neuroinflammation, Daniel Hwang, Larissa Ishikawa, Maryam S. Seyedsadr, Elisabeth R. Mari, Ezgi Kasimoglu, Ziver Sahin, Alexandra Boehm, Soohwa Jang, Javad Rasouli, Courtney Vaccaro, Michael Gonzalez, Hakon Hakonarson, Mohamad Rostami, Guang-Xian Zhang, Bogoljub Ciric

Department of Neurology Faculty Papers

GATA-binding factor 1 (GATA1) is a transcription factor that governs the development and function of multiple hematopoietic cell lineages. GATA1 is expressed in hematopoietic stem and progenitor cells (HSPCs) and is essential for erythroid lineage commitment; however, whether it plays a role in hematopoietic stem cell (HSC) biology and the development of myeloid cells, and what that role might be, remains unclear. We initially set out to test the role of eosinophils in experimental autoimmune encephalomyelitis (EAE), a model of central nervous system autoimmunity, using mice lacking a double GATA-site (ΔdblGATA), which lacks eosinophils due to the deletion of the …

The Role Of Chitinase A In Mastitis-Associated Escherichia Coli Pathogenesis, Weston D. Hutchison

Theses and Dissertations

Bovine mastitis is a common disease among dairy cattle characterized by the inflammation of the udders and loss of milk production. Mastitis-associated Escherichia coli (MAEC) are frequent causes of the disease, but the features that distinguish them from other E. coli strains remain enigmatic. MAEC infections can range from sub-clinical to severe, acute cases that can be fatal. Historically, the severity of mastitis has been attributed to host factors but more recently, a few bacterial genes have been shown to contribute to virulence in mastitis infections. In a large-scale genomic analysis of >100 MAEC isolates the gene for Chitinase A …

Decreasing Trash In Local Creeks: A Program Evaluation Of The City Of San Jose’S Direct Discharge Trash Control Program, Lakeisha Bryant

Master's Projects

The entire San Francisco Bay was once a navigable waterway in the 1850s during the Gold Rush era. Large amounts of sediment from upstream erosion and mining flowed to the bay resulting in the downsizing of the bay’s square miles (Environmental Protection Agency, 2022). As a result of intense development on the bay shores and adjacent lands, the bay faces several challenges that affect its water quality and threatens aquatic ecosystems. Pesticides, mercury, metals, and pathogens are just a few substances in the bay that cause unhealthy conditions for aquatic life and threaten human health. California’s Water Resources Control Board …

Association Of Fabry Disease With Hearing Loss, Tinnitus, And Sudden Hearing Loss: A Nationwide Population-Based Study, Yen-Fu Cheng, Sudha Xirasagar Mbbs., Ph.D., Chin-Shyan Chen, Dau-Ming Niu, Herng-Ching Lin

Faculty Publications

Hearing loss and the related otologic manifestations are receiving increased scrutiny as significant causes of morbidity in Fabry disease. However, the relative risks of auditory deficits among patients with Fabry disease relative to the general population without a diagnosis of Fabry disease have not been studied. This study aims to explore the associations between Fabry disease and hearing-related manifestations using a nationwide population-based dataset. We identified study patients for this cross-sectional study from the 2015–2017 claims databases of the Taiwan Longitudinal Health Insurance Database 2005. We first identified 2312 patients aged over 20 years with a diagnosis of Fabry disease. …

Newsroom, Georgia Southern University

Newsroom (2006-2023)

• A Graduate of Firsts: Honors College Double Major and Navy Intelligence Analyst Emma Williams Crosses New Frontiers
• ‘Instead of Retiring, I’m Refiring,’ Says Public Health Advocate Who Earns Georgia Southern Doctoral Degree this Week
• From Georgia Southern to Aerojet Rocketdyne: December Graduate Finds Her Wings
• Georgia Southern Graduate Finds Career at Top Cybersecurity Consulting Firm
• Aspiring Lawyer Becomes Georgia Southern Graduate at 17
• ‘It’s Like Something from a Movie’ for a Pair of Graduating Eagle Singers

Complicating The Narrative With The Adoption Constellation, Amanda K. Mchugh

Capstones

From September 2021 through December 2022 I worked with birth mothers and adoptees who are making efforts to complicate the narrative around adoption online. This resulted in a multi-platform project, creating a highly successful TikTok channel, an Instagram page, a private Facebook group and a Substack newsletter. The platforms decided on as well as the content on these platforms were decided upon in collaboration with the community, using deep listening and collecting both qualitative and quantitative data. I conducted six callouts through each ideation phase of the project, learning about where the media is falling short with regards to information …

Consensus Opinion From An International Group Of Experts On Measurable Residual Disease In Hairy Cell Leukemia, Farhad Ravandi, Robert J Kreitman, Enrico Tiacci, Leslie Andritsos, Versha Banerji, Jacqueline C Barrientos, Seema A Bhat, James S Blachly, Alessandro Broccoli, Timothy Call, Dai Chihara, Claire Dearden, Judit Demeter, Sasha Dietrich, Monica Else, Narendranath Epperla, Brunangelo Falini, Francesco Forconi, Douglas E Gladstone, Alessandro Gozzetti, Sunil Iyengar, James B Johnston, Jeffrey Jorgensen, Gunnar Juliusson, Francesco Lauria, Gerard Lozanski, Sameer A Parikh, Jae H Park, Aaron Polliack, Graeme Quest, Tadeusz Robak, Kerry A Rogers, Alan Saven, John F Seymour, Tamar Tadmor, Martin S Tallman, Constantine S Tam, Philip A Thompson, Xavier Troussard, Clive S Zent, Thorsten Zenz, Pier Luigi Zinzani, Bernhard Wörmann, Kanti Rai, Michael Grever

Faculty, Staff and Student Publications

A significant body of literature has been generated related to the detection of measurable residual disease (MRD) at the time of achieving complete remission (CR) in patients with hairy cell leukemia (HCL). However, due to the indolent nature of the disease as well as reports suggesting long-term survival in patients treated with a single course of a nucleoside analog albeit without evidence of cure, the merits of detection of MRD and attempts to eradicate it have been debated. Studies utilizing novel strategies in the relapse setting have demonstrated the utility of achieving CR with undetectable MRD (uMRD) in prolonging the …

Defining Cellular Population Dynamics At Single-Cell Resolution During Prostate Cancer Progression, Alexandre A Germanos, Sonali Arora, Ye Zheng, Erica T Goddard, Ilsa M Coleman, Anson T Ku, Scott Wilkinson, Hanbing Song, Nicholas J Brady, Robert A Amezquita, Michael Zager, Annalysa Long, Yu Chi Yang, Jason H Bielas, Raphael Gottardo, David S Rickman, Franklin W Huang, Cyrus M Ghajar, Peter S Nelson, Adam G Sowalsky, Manu Setty, Andrew C Hsieh

Faculty, Staff and Student Publications

Advanced prostate malignancies are a leading cause of cancer-related deaths in men, in large part due to our incomplete understanding of cellular drivers of disease progression. We investigate prostate cancer cell dynamics at single-cell resolution from disease onset to the development of androgen independence in an in vivo murine model. We observe an expansion of a castration-resistant intermediate luminal cell type that correlates with treatment resistance and poor prognosis in human patients. Moreover, transformed epithelial cells and associated fibroblasts create a microenvironment conducive to pro-tumorigenic immune infiltration, which is partially androgen responsive. Androgen-independent prostate cancer leads to significant diversification of …

Mir21 Modulates The Hippo Signaling Pathway Via Interference With Pp2a Bβ To Inhibit Trophoblast Invasion And Cause Preeclampsia, Mingyu Hu, Yangxi Zheng, Jiujiang Liao, Li Wen, Juan Cheng, Jiayu Huang, Biao Huang, Li Lin, Yao Long, Yue Wu, Xuan Ye, Yong Fu, Hongbo Qi, Philip N Baker, Chao Tong

Faculty, Staff and Student Publications

Preeclampsia (PE) is a pregnancy-specific disorder attributed to deficient extravillous trophoblast (EVT) invasion into the uterus, but the mechanism of EVT invasion remains unclear. In this study, we found significantly elevated expression of microRNA 21 (miR21), which negatively regulates trophoblast invasion and migration, in preeclamptic placentae. Whole-genome RNA sequencing revealed that PPP2R2B, which encodes PP2A Bβ, and the Hippo pathway are downstream targets of miR21. The effects of miR21 on trophoblast mobility were abolished in LATS1T1079A/S909A and YAP-5SA mutants. Moreover, we found that PP2A Bβ dephosphorylates LATS1 via direct protein-protein interactions and thus modulates the phosphorylation and subcellular distribution …

Psychoactive Space: Glimpses Of The Unknown, Mathew Emmett

School of Art, Design and Architecture

No abstract provided.

Servants Of The Times: Process-Relation And Complexity In Servant First Leadership, Michael T. Von Behren

International Journal of Servant-Leadership

A bus seat in the first Colored row, an unusual flood of White commuters, a testy bus driver, a recently minted young minister, and a U.S. court order for desegregation, none of these are static items. Each is a happening in itself. Each is the product of a precipitating flood of moments leading up to an event or more than an event, a protracted event that history has dubbed the Montgomery Bus Boycott. On the other side of the 381-day ordeal Martin Luther King Jr. emerged as the nationally recognized leader of the Southern Christian Leadership Council (SCLC) (Williams, 1996/2009). …

Egfr-Phosphorylated Gdh1 Harmonizes With Rsk2 To Drive Creb Activation And Tumor Metastasis In Egfr-Activated Lung Cancer, Jihoon Kang, Jaemoo Chun, Jung Seok Hwang, Chaoyun Pan, Jie Li, Austin C Boese, Isabelle Young, Courteney M Malin, Yibin Kang, Don L Gibbons, Gabriel Sica, Haian Fu, Suresh S Ramalingam, Lingtao Jin, Sumin Kang

Faculty, Staff and Student Publications

The cancer metastasis process involves dysregulated oncogenic kinase signaling, but how this orchestrates metabolic networks and signal cascades to promote metastasis is largely unclear. Here we report that inhibition of glutamate dehydrogenase 1 (GDH1) and ribosomal S6 kinase 2 (RSK2) synergistically attenuates cell invasion, anoikis resistance, and immune escape in lung cancer and more evidently in tumors harboring epidermal growth factor receptor (EGFR)-activating or EGFR inhibitor-resistant mutations. Mechanistically, GDH1 is activated by EGFR through phosphorylation at tyrosine 135 and, together with RSK2, enhances the cAMP response element-binding protein (CREB) activity via CaMKIV signaling, thereby promoting metastasis. Co-targeting RSK2 and GDH1 …

One Man's Pregnancy Story, Yvonne S. Marquez, Paige A. Perez

Capstones

Pregnancy is a challenging time for anyone. For LGBTQ people there are unique obstacles that make it especially difficult to navigate a medical system that was not built for them. Finn Schubert is trans and six months pregnant. He’s loving the experience but stressed by insurance claim denials, concerns for his safety, and medical incompetence.

This capstone project includes a 20-minute documentary, text, and a photo essay.

Respons Pertumbuhan Biomassa Bagian Atas Bibit Kelapa Sawit Akibat Pemberian Kompos Pelepah Kelapa Sawit, Pupuk Hayati, Dan Asam Humat, Mira Ariyanti, Esnakelga Bernadetha Keliat, Cucu Suherman, Santi Rosniawaty, Mochamad Arief Soleh

Jurnal Kultivasi

Pemberian pupuk anorganik dapat meningkatkan pertumbuhan tanaman, tetapi penggunaan secara terus menerus menyebabkan dampak kurang baik bagi tanaman dan lingkungan pertanaman. Penelitian ini berkaitan dengan pentingnya penggunaan pupuk organik dalam mengurangi penggunaan pupuk anorganik sebagai salah satu langkah untuk mewujudkan perkebunan kelapa sawit (Elaeis guineensis Jacq.) yang berkelanjutan. Tujuan penelitian ini adalah untuk mengetahui respons pertumbuhan biomassa bagian atas bibit kelapa sawit akibat pemberian kompos pelepah kelapa sawit yang dikombinasikan dengan pupuk hayati dan asam humat. Percobaan dilaksanakan di Kebun Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran pada bulan Januari – Juli 2020. Penelitian disusun menggunakan Rancangan Acak Kelompok (RAK) dengan …

Respon Pertumbuhan Tanaman Krisan Pot Terhadap Hormon Giberelin Dan Waktu Pemangkasan Yang Berbeda, Galuh Wulan Andini, Refa Firgiyanto

Jurnal Kultivasi

Salah satu teknologi yang dapat digunakan untuk meningkatkan kualitas tanaman krisan (Dendrathema grandifloria) pot yaitu dengan pemberian giberelin dan pengaturan waktu pemangkasan. Tujuan pemberian giberelin dan pengaturan waktu pemangkasan adalah untuk memacu pertumbuhan dengan merangsang perpanjangan batang serta memunculkan tunas lateral sehingga memperbanyak percabangan yang akhirnya dapat menghasilkan banyak kuntum bunga. Penelitian ini bertujuan untuk mendapatkan konsentrasi giberelin dan waktu pemangkasan yang terbaik. Penelitian dilaksanakan pada 3 Juli sampai 4 September 2020 di Greenhouse Rembangan, Desa Kemuning Lor, Kecamatan Arjasa, Jember. Rancangan percobaan yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) Faktorial dengan 2 faktor yang diulang 3 …

Scientology's Faq: Sciencing, Tech-Romancing, Simulacra, & Simulation An Estranged Content Analysis, David Scott Morrill

Dissertations and Theses

This thesis examines how the practice of sciencing, tech-romancing, simulacra, and simulation manifests in both the text and symbols used on audiences of the Church of Scientology’s outreach materials. It specifically examines its official FAQ page. Self-framing was observed that satisfied the definition of sciencing and tech-romancing. Under an Estranged approach, Morrill examined selections of the Church’s FAQ pages, coding for instances of sciencing, tech-romancing, simulacra, and simulation. The data indicate that the Church frequently adopts simulacra and engages in simulation. The Church was seen frequently adopting technological and scientific symbols and jargon. The degree of sciencing, tech-romancing, simulacra, and …

Condensates Formed By Prion-Like Low-Complexity Domains Have Small-World Network Structures And Interfaces Defined By Expanded Conformations, Mina Farag, Samuel R Cohen, Wade M Borcherds, Anne Bremer, Tanja Mittag, Rohit V Pappu

2020-Current year OA Pubs

Biomolecular condensates form via coupled associative and segregative phase transitions of multivalent associative macromolecules. Phase separation coupled to percolation is one example of such transitions. Here, we characterize molecular and mesoscale structural descriptions of condensates formed by intrinsically disordered prion-like low complexity domains (PLCDs). These systems conform to sticker-and-spacers architectures. Stickers are cohesive motifs that drive associative interactions through reversible crosslinking and spacers affect the cooperativity of crosslinking and overall macromolecular solubility. Our computations reproduce experimentally measured sequence-specific phase behaviors of PLCDs. Within simulated condensates, networks of reversible inter-sticker crosslinks organize PLCDs into small-world topologies. The overall dimensions of PLCDs …

Tissue Registration And Exploration User Interfaces In Support Of A Human Reference Atlas, Katy Börner, Andreas Bueckle, Bruce W Herr 2nd, Leonard E Cross, Ellen M Quardokus, Elizabeth G Record, Yingnan Ju, Jonathan C Silverstein, Kristen M Browne, Sanjay Jain, Clive H Wasserfall, Marda L Jorgensen, Jeffrey M Spraggins, N Heath Patterson, Griffin M Weber

2020-Current year OA Pubs

Seventeen international consortia are collaborating on a human reference atlas (HRA), a comprehensive, high-resolution, three-dimensional atlas of all the cells in the healthy human body. Laboratories around the world are collecting tissue specimens from donors varying in sex, age, ethnicity, and body mass index. However, harmonizing tissue data across 25 organs and more than 15 bulk and spatial single-cell assay types poses challenges. Here, we present software tools and user interfaces developed to spatially and semantically annotate ("register") and explore the tissue data and the evolving HRA. A key part of these tools is a common coordinate framework, providing standard …