Digital Commons Network^™

“One Bug, Two Bug, Good Bug, Bad Bug”, Ivy Grob

Department of Entomology: Distance Master of Science Projects

Integrated Pest Management (IPM) is a holistic strategy for the management of pest species. It is a framework that relies on scientifically informed decisions to identify and remediate a pest problem in a way that minimizes harm to the environment, human health, and the economy (Dara, 2019). The term pest is broadly defined not just as a nuisance, but as any organism that has detrimental effects to a resource or public health and that exists at a population level that exceeds a threshold of economic damage. This encompasses invertebrates (such as insects, nematodes, and mites), vertebrates, plant pathogens, diseases, and …

A Guide To Agricultural Insect Pests On The East Coast, Devan Mazza-Mcdonald

Department of Entomology: Distance Master of Science Projects

A Guide to Agricultural Insect Pests on the East Coast is a useful resource comprised of fact sheets of ten invasive pests that have been introduced to the United States. It also includes a fact sheet of three important pollinator species that are of economic and environmental importance. Most of the pests I selected are currently an issue in states on the East coast and in some instances, further west as well. Currently, the khapra beetle is not a problem pest in the United States but does have a possibility of becoming an issue, again. The invasive pests I chose …

The Collegiate Black Space: Black College Students’ Use Of New Counter-Spaces For Support, Knowledge Production, And Organizing For Activism, Heather Streets

Doctoral Dissertations

Black collegians who attend historically white institutions continue to struggle with racism, microaggressions, feelings of alienation, minimal or improper advising, and an undue pressure to prove themselves (Bonner, 2010; Feagin & Sikes, 1995; Strayhorn, 2010). These barriers to success result in part due to a lack of support from the colleges and universities that they attend (Allen, 1992; Parker, Puig, Johnson & Anthony, Jr., 2016). With institutional benefits designed to benefit white students over students of color, Black students must find their own alternatives for collaboration and to provide support for their peers.

Many Black spaces can be defined as …

Gc/Ms And Lc/Ms Approaches To Chemical Communication In Plants And Marine Cyanobacteria, Carolyn Keim

University of the Pacific Theses and Dissertations

Natural products are small organic compounds synthesized by living organisms including plants, animals, and microbes. These fall into one of two categories, primary metabolites are required for survival, but secondary metabolites usually play a more accessory role. Extracts from organisms have proven to be useful to humans throughout history. Prior to evidence-based western medicine practices, natural products were the only source of medicine and remain as a treatment source for underdeveloped nations. Today, natural products have either been approved by or have served as the template for many FDA approved drugs. Based on a survey of all FDA approved drugs …

The Effect Of Acute And Chronic Arsenic Exposure On Malignant Transformation Of The Human Bronchial Epithelial Cell (Beas-2b), Bandar Almutairy

Wayne State University Dissertations

Arsenic (As3+), a metalloid abundant in the environment, is classified as a group I carcinogen associated with several common human cancers, including cancers in the lung, skin, bladder, liver, and prostate (Wei, Zhang & Tao, 2019b). The mechanisms of As3+-induced carcinogenesis had been extensively studied, and different mechanisms might be involved in various types of cancer (Wei, Zhang & Tao, 2019b). Recent studies showed that exposure to a high dose of arsenic is able to induce lung cancer. Moreover, arsenic activates oncogenic signaling pathways such as MAPKs, EGFR/RAS/RAF, PI3K/AKT, and JNK/STAT3 pathways as well as epigenetic alterations such as miRNAs …

Vaginal Microbiota Of Recurrent Bacterial Vaginosis Patients Under Oral Metronidazole Therapy: Prognosis Based On Specific Species Of Gardnerella And Other Bacterial Vaginosis Associated Species In Association With The Vaginal Microbiome, Ashomathi Ashok Sudhamathi Mollin

Wayne State University Theses

Bacterial Vaginosis (BV) is a state of vaginal dysbiosis in which BV-associated species displace the typically dominant species of Lactobacillus. This urogenital disease elevates the risk for complications such as pre-term birth, STDs and HIV. Currently, there is no consensus on the etiology of BV. Standard of care treatment is oral metronidazole; however, therapy has high rates of recurrence. Our previous qPCR study of recurrent BV patients (3+ episodes/year) found strong associations of Gardnerella_Gsp07, G. swidsinksii, and G. leopoldii with refractory and recurrent responses, and a robust recovery of Lactobacillus in remission patients. However, these findings were not applicable across …

Expanding Civil Rights To Combat Digital Discrimination On The Basis Of Poverty, Michele Estrin Gilman

SMU Law Review

Low-income people suffer from digital discrimination on the basis of their socio-economic status. Automated decision-making systems, often powered by machine learning and artificial intelligence, shape the opportunities of those experiencing poverty because they serve as gatekeepers to the necessities of modern life. Yet in the existing legal regime, it is perfectly legal to discriminate against people because they are poor. Poverty is not a protected characteristic, unlike race, gender, disability, religion or certain other identities. This lack of legal protection has accelerated digital discrimination against the poor, fueled by the scope, speed, and scale of big data networks. This Article …

Addressing Gender-Based Violence Using Evidence-Based Practices During Covid-19: The Case Of Puerto Rico, Ayorkor Gaba, Roseanne Flores, María Rebecca Ward, Bailey Pridgen

Publications and Research

As gender-based violence (GBV) surged during the COVID-19 pandemic, the 65th session of the Commission on the Status of Women (CSW65) called for member states, civil, and other stakeholders to consider the specific needs of women and girls in COVID-19 response and recovery efforts. Psychology provides scientific knowledge to help answer this call. Despite existing global guidance and psychological research to mitigate GBV, COVID-19 presents new challenges for consideration. This article summarizes existing GBV guidance/research and COVID-19 considerations, uses an illustrative case study to describe Puerto Rico’s application of GBV guidance/research during COVID-19, and provides preliminary policy and practice recommendations.

Distribution Of Toxicity Of Plant Species In The Plant Family Sapindaceae (Sub-Family Hippocastanoideae), Vishvam Mehta

Undergraduate Honors Thesis Projects

Maple leaf toxicosis is a lethal condition in which wilted leaves from the plant genus Acer are ingested by equines, leading to hemolytic anemia and methemoglobinemia. In prior studies, the toxicity of various Acer species was quantified, revealing a wide array of responses in terms of hemolysis and methemoglobin production. The object of this study, therefore, is to determine the toxicity of Acer’s closest relatives within the family Sapindaceae, specifically the four additional genera in the subfamily Hippocastanoideae. The information gained from this study will be used to understand the evolution of the toxins responsible for hemolysis and methemoglobin production. …

Repeat Anterior Cruciate Ligament Injury And Return To Sport In Australian Soccer Players After Anterior Cruciate Ligament Reconstruction With Hamstring Tendon Autograft, Jonathan R. Manara, Lucy J. Salmon, Faisal M. Kilani, Gerardo Zelaya De Camino, Claire Monk, Keran Sundaraj, Leo A. Pinczewski, Justin P. Roe

Medical Papers and Journal Articles

Background: Soccer is the most commonly played team sport in the world and a high-risk sport for anterior cruciate ligament (ACL) injury and subsequent ACL reconstruction (ACLR).

Purpose: To assess the rate of further ACL injury in patients who have undergone ACLR with hamstring tendon autograft after soccer injuries in Australia and to determine factors associated with repeat ACL injury and return to soccer.

Study Design: Case-control study; Level of evidence, 3.

Methods: From a prospectively collected database, a series of 1000 consecutive ACLRs using hamstring autografts performed in soccer players were identified. Patients were surveyed at a minimum 5 …

Delirium In Hospitalised Adults With Acute Burns – A Systematic Review, Guy H.M Stanley, Angus R.J Barber, Aoife M. O'Brien, Cheryl Hamill, Glenn Boardman, Cody C. Frear, Dale W. Edgar, Hannah Seymour, Fiona M. Wood

IHR Papers and Journal Articles

Introduction: Delirium is a potentially modifiable, acutely altered mental state, commonly characterised as a hospital-acquired complication. Studies of adult inpatients with acute burns with and without delirium identify causative risks related to the injury or treatment and outcomes related to the patient and healthcare system. We compare patients with and without delirium, providing a high-level quantitative synthesis of delirium risks and outcomes to inform guidelines and future research.

Methods: A systematic review, meta-analysis and GRADE evaluation of risks and outcomes associated with delirium in adults with acute burns was conducted using PRISMA guidelines and PROSPERO protocol CRD42021283055. The Newcastle-Ottawa Scale …

A Differential Response To Antihypertensive Therapy In African Men And Women: Insights From The Creole Trial, Dike B. Ojji, Gabriel L. Shedul, Mahmoud Sani, Okechukwu S. Ogah, Anastase Dzudie, Felix Barasa, Charles Mondo, Prossie M. Ingabire, Erika S.W. Jones, Brian Rayner, Damasceno Albertino, Elijah Ogola, Wynand Smythe, Nicky Hickman, Veronica Francis, Pandie Shahiemah, Grace Shedul, Akinyemi Aje, Karen Sliwa, Simon Stewart, For The Creole Investigators

IHR Papers and Journal Articles

Background: We sought to address the paucity of data to support the evidence-based management of hypertension to achieve optimal blood pressure (BP) control on a sex-specific basis in Africa.

Methods: We undertook a post hoc analysis of the multicenter, randomized CREOLE (Comparison of Three Combination Therapies in Lowering Blood Pressure in Black Africans) Trial to test the hypothesis that there would be clinically important differences in office BP control between African men and women. We compared the BP levels of 397 and 238 hypertensive women (63%, 50.9 ± 10.5 years) and men (51.2 ± 11.3 years) from 10 sites across …

Childhood And Infant Exposure To Famine In The Biafran War Is Associated With Hypertension In Later Life: The Abia Ncds Study, Okechukwu S. Ogah

IHR Papers and Journal Articles

There are very few studies in Africans investigating the association between early life exposure to malnutrition and subsequent hypertension in adulthood. We set out to investigate this potential association within an adult cohort who were born around the time of the Biafran War (1968–1970) and subsequent famine in Nigeria. This was a retrospective analysis of Abia State NonCommunicable Diseases and Cardiovascular Risk Factors (AS-NCD-CRF) Survey, a community-based, cross-sectional study that profiled 386 adults (47.4% men) of Igbo ethnicity born in the decade between January 1965 and December 1974. Based on their date of birth and the timing of the famine, …

Editorial: Insights In Vector Biology: 2021, Baldwyn Torto, David P. Tchouassi

All Peer-Reviewed Publications

This editorial summarizes collections on the Research Topic “Insights in Vector Biology’’. Vectors of four major tropical diseases including malaria, Chagas, dengue and West Nile were considered. The editorial discusses vectors contributing to residual malaria transmission that reside mainly outdoors and not targeted by indoor vector control interventions, notably, long lasting insecticidal nets and indoor residual spraying; the concept of paratransgenesis- a parasite transmission blocking strategy which uses genetically-modified microbes made to produce antimalarial effectors inside the vector for malaria control; assessment of insecticide resistance in malaria vectors; insecticide resistance in Chagas disease vectors; reproductive biology of the dengue vector; …

Potential Of Entomocomposting Toward Soil Pathogen Suppression, Dennis Beesigamukama, María Gómez-Brandón, Chrysantus Mbi Tanga

All Peer-Reviewed Publications

Waste accumulation and microbial pollution are key environmental challenges in most developing countries. Land filling, the commonest waste disposal method, is not sustainable due to land scarcity and point source pollution. Thus, a large fraction of organic wastes is not properly disposed, yet the conventional methods of waste recycling are less effective. The degradation of waste using insect life stages (entomocomposting) has emerged as a novel tool that is fast, affordable, and sustainable in recycling organic wastes with maximum pathogen suppression. This bioconversion process also generates an organic fertilizer with potential to enhance soil remediation and provide additional benefits on …

Microsporidia: A Promising Vector Control Tool For Residual Malaria Transmission, Tullu Bukhari, Roland Pevsner, Jeremy Keith Herren

All Peer-Reviewed Publications

Long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) have resulted in a major decrease in malaria transmission. However, it has become apparent that malaria can be effectively transmitted despite high coverage of LLINs/IRS. Residual transmission can occur due to Plasmodium-carrying Anopheles mosquitoes that are insecticide resistant and have feeding and resting behavior that reduces their chance of encountering the currently deployed indoor malaria control tools. Residual malaria transmission is likely to be the most significant hurdle to achieving the goal of malaria eradication and research and development towards new tools and strategies that can control residual malaria transmission is …

Effect Of Desmodium Intortum And Black Soldier Fly Larvae (Hermetia Illucens) Based Meal On Sensory And Physicochemical Properties Of Broiler Chicken Meat In Kenya, M. M. Mutisya, S. B.S. Baleba, J. N. Kinyuru, C. M. Tanga, M. Gicheha, G. Hailu, D. Salifu, J. Egonyu, X. Cheseto, S. Niassy

All Peer-Reviewed Publications

We evaluated the effect of dietary inclusion of different ratios of Desmodium intortum (DI) and black soldier fly larvae meal (BSFLM) on sensory traits and physicochemical qualities of broiler chicks meat. Three formulations: T1:75%BSFL:25%DI; T2:50%BSFL:50DI, and T3:25BSFL:75% DI as a protein source were tested. A conventional feed was used as the control diet. On day 42, the chickens were slaughtered, and thigh muscles were used for sensory and physicochemical evaluations. T1 was preferred for its flavour and tenderness, T2 for its juiciness and T3 for its colour, flavour, taste and overall acceptability scores. In terms of physical properties, all three …

Performance Of Black Soldier Fly Frass Fertiliser On Maize (Zea Mays L.) Growth, Yield, Nutritional Quality, And Economic Returns, C. M. Tanga, D. Beesigamukama, M. Kassie, P. J. Egonyu, Changeh J. Ghemoh, Kiatoko Nkoba, S. Subramanian, A. O. Anyega, S. Ekesi

All Peer-Reviewed Publications

Although the black soldier fly frass fertiliser (BSFFF) is globally recognised as a promising and potential high-quality organic fertiliser, there is inadequate information on its impact on the growth performance and nutritional status of maize. Furthermore, no information exist on their combination with mineral fertiliser (NPK) as well as economic impact on maize production. This study evaluated the comparative impact of BSFFF, NPK and commercial organic fertiliser (Evergrow®) on growth, nitrogen use efficiency, yield, nutritional quality, and profitability of maize under greenhouse conditions. The treatments included: (1) sole application of BSFFF, conventional compost of brewers’ spent grain (BSG), Evergrow, and …

How Do Perceptions Of Risk Communicator Attributes Affect Emergency Response? An Examination Of A Water Contamination Emergency In Boston, Usa, Amy Hyman, Sudha Arlikatti, Shih-Kai Huang, Michael K. Lindell, Jeryl Mumpower, Carla S. Prater, Hao-Che Wu

Research, Publications & Creative Work

A water main break that contaminated the Boston area's water distribution system prompted a four-day “boil water” order. To understand risk communication during this incident, 600 randomly sampled residents were mailed questionnaires, yielding 110 valid responses. This article describes how perceptions of different social stakeholders influenced whether respondents complied with the Protective Action Recommendation—PAR (i.e., drank boiled water), took alternative protective actions (i.e., drank bottled water or/and self-chlorinated water), or ignored the threat (i.e., continued to drink untreated tap water). Respondents perceived technical authorities (i.e., water utility, public health, and emergency management) to be higher on three social influence attributes …

Engineered Cord Blood Megakaryocytes Evade Killing By Allogeneic T-Cells For Refractory Thrombocytopenia, Bijender Kumar, Vahid Afshar-Kharghan, Mayela Mendt, Robert Sackstein, Mark R Tanner, Uday Popat, Jeremy Ramdial, May Daher, Juan Jimenez, Rafet Basar, Luciana Melo Garcia, Mayra Shanley, Mecit Kaplan, Xinhai Wan, Vandana Nandivada, Francia Reyes Silva, Vernikka Woods, April Gilbert, Ricardo Gonzalez-Delgado, Sunil Acharya, Paul Lin, Hind Rafei, Pinaki Prosad Banerjee, Elizabeth J Shpall

Faculty, Staff and Student Publications

The current global platelet supply is often insufficient to meet all the transfusion needs of patients, in particular for those with alloimmune thrombocytopenia. To address this issue, we have developed a strategy employing a combination of approaches to achieve more efficient production of functional megakaryocytes (MKs) and platelets collected from cord blood (CB)-derived CD34+ hematopoietic cells. This strategy is based on ex-vivo expansion and differentiation of MKs in the presence of bone marrow niche-mimicking mesenchymal stem cells (MSCs), together with two other key components: (1) To enhance MK polyploidization, we used the potent pharmacological Rho-associated coiled-coil kinase (ROCK) inhibitor, KD045, …

Seasonal Factors Affecting Sea Turtle Nesting In The Southeastern Caribbean Sea (Gulf Of Paria, Venezuela), Clemente Balladares, Digna T. Rueda-Roa, Diego Rodríguez, Frank E. Muller-Karger, Hector Barrios-Garrido

Marine Science Faculty Publications

The nesting characteristics (number of nests and eggs, time of year, nesting initiation, and nesting length) of leatherback (Dermochelys coriacea) and hawksbill (Eretmochelys imbricata) sea turtles of the southern Caribbean Sea (specifically in the Gulf of Paria in Venezuela), were examined in association with weekly precipitation averages and number of rainy days per week during the period between 2009 and 2018. We hypothesized about the influence of rainfall intensity and patterns as the main abiotic factor for sea turtle nesting. On average, leatherbacks preferred nesting during the drier season of each year (March, April, and May), …

A Comparative Test Of Creative Thinking In Preschool Children And Dolphins, Dawn Melzer, Deirdre Yeater, Madison Bradley, Heather M. Hill, Gonzalo Guerra, Kimberly Salazar, Teresa Bolton, Kathleen M. Dudzinski

Psychology Faculty Publications

Creativity is considered one aspect of intelligence. Including creativity allows for more room for expression (e.g., participants can respond with movement instead of written or verbal responses) than in standard intelligence assessments. The Torrance Tests of Creative Thinking (TTCT; Torrance, 1974) are the leading method of assessing creative abilities in school-aged humans and above. To assess creativity in young humans and nonhuman animals, modifications must be made to facilitate nonverbal responses. In the current study, a cross-species comparison was conducted between preschoolers and bottlenose dolphins to examine responses to a modified creativity task in which both species were trained to …

Aneuploidy And Sex Concordance Rate Between Cell-Free Dna Analysis From Spent Culture Media Of Preimplantation Embryo And Dna From Whole Embryo With Respect To Different Morphological Grading, Hiroki Sonehara, Ryoya Matsumoto, Naoki Nakayama, Masato Kobanawa, Koki Numata, Akiko Kawasaki, Makio Shozu

Faculty, Staff and Student Publications

PURPOSE: The aneuploidy and sex concordance between cell-free DNA in spent culture media (SCM) and DNA from whole embryo with respect to different morphological grading were examined to evaluate the feasibility of non-invasive preimplantation genetic testing for aneuploidy (niPGT-A).

METHODS: A total of 46 pairs of embryos and corresponding SCM were divided into two groups based on the morphological grade. DNA was extracted from 22 and 24 pairs of low- and high-grade embryos, respectively, and respective SCM followed by chromosomal analysis using next-generation sequencing. Aneuploidy study and sex determination were conducted for both groups, and concordance rates were calculated.

RESULTS: …

Regenerative Medicine Technologies Applied To Transplant Medicine An Update, Astgik Petrosyan, Filippo Montali, Andrea Peloso, Antonio Citro, Lori N Byers, Catherine La Pointe, Mara Suleiman, Alice Marchetti, Eoin P Mcneill, Allison L Speer, Wai Hoe Ng, Xi Ren, Benedetta Bussolati, Laura Perin, Paolo Di Nardo, Vincenzo Cardinale, Jerome Duisit, Alexandra Rose Monetti, John Richard Savino, Amish Asthana, Giuseppe Orlando

Faculty, Staff and Student Publications

Regenerative medicine (RM) is changing how we think and practice transplant medicine. In regenerative medicine, the aim is to develop and employ methods to regenerate, restore or replace damaged/diseased tissues or organs. Regenerative medicine investigates using tools such as novel technologies or techniques, extracellular vesicles, cell-based therapies, and tissue-engineered constructs to design effective patient-specific treatments. This review illustrates current advancements in regenerative medicine that may pertain to transplant medicine. We highlight progress made and various tools designed and employed specifically for each tissue or organ, such as the kidney, heart, liver, lung, vasculature, gastrointestinal tract, and pancreas. By combing both …

Case Report: Novel Pathogenic Variant In Nfix In Two Sisters With Malan Syndrome Due To Germline Mosaicism, Elizabeth Langley, Laura S Farach, Kate Mowrey

Faculty, Staff and Student Publications

Malan syndrome is an autosomal dominant disorder caused by pathogenic variants in NFIX with less than 100 cases reported thus far. NFIX is important for stem cell proliferation, quiescence, and differentiation during development and its protein plays a role in replication, signal transduction, and transcription. As a result of pathogenic variants, symptoms of Malan syndrome include overgrowth, intellectual disability, speech delay, and dysmorphic features. Currently, the recurrence risk for this disorder is indicated at less than 1%, standard for de novo autosomal dominant disorders. Herein, we report an additional set of sisters with the same novel pathogenic variant in NFIX …

The Psychological Impact Of The Covid-19 Outbreak On Pregnancy And Mother-Infant Prenatal Bonding, Muhammad O Awiwi, Khaled M Elsayes, Yehia I Mohamed, Lina Altameemi, Migena Gjoni, Omayr Muhammad Irshad, Ahmed Sayed Ahmed, Ahmad O Kaseb, Usama Salem

Faculty, Staff and Student Publications

PURPOSE: To identify prognostic clinical and radiologic features in patients with unresectable hepatocellular carcinoma (HCC) treated with atezolizumab plus bevacizumab.

PATIENTS AND METHODS: Clinical and imaging records of patients with unresectable HCC were retrospectively reviewed, and baseline features were recorded. Patients' records and imaging studies were used to determine the patients' overall survival (OS) and progression-free survival (PFS). Univariate and multivariate analyses were performed to determine prognostic features. Subanalyses of treatment-naïve patients (who never received local or systemic therapy) and previously treated patients were also performed.

RESULTS: Fifty-five patients were included in the final analysis, 23 (41.8%) of whom were …

Impairment Of Autophagy After Spinal Cord Injury Potentiates Neuroinflammation And Motor Function Deficit In Mice, Yun Li, Zhuofan Lei, Rodney M Ritzel, Junyun He, Hui Li, Harry M C Choi, Marta M Lipinski, Junfang Wu

Faculty, Staff and Student Publications

Autophagy is a catabolic process that degrades cytoplasmic constituents and organelles in the lysosome, thus serving an important role in cellular homeostasis and protection against insults. We previously reported that defects in autophagy contribute to neuronal cell damage in traumatic spinal cord injury (SCI). Recent data from other inflammatory models implicate autophagy in regulation of immune and inflammatory responses, with low levels of autophagic flux associated with pro-inflammatory phenotypes. In the present study, we examined the effects of genetically or pharmacologically manipulating autophagy on posttraumatic neuroinflammation and motor function after SCI in mice.

Methods: Young adult male C57BL/6, CX3CR1-GFP, autophagy …

A Haplotype Resolved Chromosomal Level Avocado Genome Allows Analysis Of Novel Avocado Genes, Onkar Nath, Stephen J Fletcher, Alice Hayward, Lindsay M Shaw, Ardashir Kharabian Masouleh, Agnelo Furtado, Robert J Henry, Neena Mitter

Faculty, Staff and Student Publications

Avocado (Persea americana) is a member of the magnoliids, an early branching lineage of angiosperms that has high value globally with the fruit being highly nutritious. Here, we report a chromosome-level genome assembly for the commercial avocado cultivar Hass, which represents 80% of the world’s avocado consumption. The DNA contigs produced from Pacific Biosciences HiFi reads were further assembled using a previously published version of the genome supported by a genetic map. The total assembly was 913 Mb with a contig N50 of 84 Mb. Contigs assigned to the 12 chromosomes represented 874 Mb and covered 98.8% of …

Single-Cell And Single-Nuclei Rna Sequencing As Powerful Tools To Decipher Cellular Heterogeneity And Dysregulation In Neurodegenerative Diseases, Raquel Cuevas-Diaz Duran, Juan Carlos González-Orozco, Iván Velasco, Jia Qian Wu

Faculty, Staff and Student Publications

Neurodegenerative diseases affect millions of people worldwide and there are currently no cures. Two types of common neurodegenerative diseases are Alzheimer's (AD) and Parkinson's disease (PD). Single-cell and single-nuclei RNA sequencing (scRNA-seq and snRNA-seq) have become powerful tools to elucidate the inherent complexity and dynamics of the central nervous system at cellular resolution. This technology has allowed the identification of cell types and states, providing new insights into cellular susceptibilities and molecular mechanisms underlying neurodegenerative conditions. Exciting research using high throughput scRNA-seq and snRNA-seq technologies to study AD and PD is emerging. Herein we review the recent progress in understanding …

Mechanisms And Treatment Of Pain-Depressed Behavior In Male And Female Mice, Clare M. Diester

Theses and Dissertations

One of the largest discrepancies between preclinical and clinical assessment of pain and analgesia is the type of behavioral endpoint used. Although most preclinical research has historically focused on drug effectiveness to block reflexive withdrawal behaviors stimulated by a noxious stimulus, increasing evidence shows that drug-induced restoration of pain-depressed behaviors provides increased clinical translation with decreased susceptibility to false-positives. Accordingly, the first portion of this dissertation designed a behavioral battery that featured pain-depressed behaviors as well as more conventional pain-stimulated behaviors for testing candidate analgesics in male and female mice. The main findings are as follows. (1) Intraperitoneal injection of …