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2022

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Photoperiod And Temperature Sensitivity In Early Soybean Accessions From The Vir Collection In Leningrad Province Of The Russian Federation, Liubov Novikova, Irina Seferova, Inna Matvienko, Zoya Shchedrina, Margarita Vishnyakova Jan 2022

Photoperiod And Temperature Sensitivity In Early Soybean Accessions From The Vir Collection In Leningrad Province Of The Russian Federation, Liubov Novikova, Irina Seferova, Inna Matvienko, Zoya Shchedrina, Margarita Vishnyakova

Turkish Journal of Agriculture and Forestry

The photoperiod and temperature sensitivity of the onset of flowering in 106 soybean accessions from the VIR collection has been studied for 8 years in a vegetation experiment in the conditions of Leningrad Province at 59°N, the northernmost point of soybean evaluation in the world. The accessions were previously field-assessed for the ability to form fully developed seeds in this region. The experimental conditions were extreme for soybean in terms of both photoperiod and heat supply. The emergence to flowering period length (LEF) in the sample set was 7.9 days longer, and the sum of the temperatures required …

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Development Of Dna Markers Associated With Sunburn Resistance In Pomegranate (Punica Granatum L.) Using Bulk Segregant Analysis, Kezi̇ban Yazici, Mehtap Şahi̇n Çevi̇k Jan 2022

Development Of Dna Markers Associated With Sunburn Resistance In Pomegranate (Punica Granatum L.) Using Bulk Segregant Analysis, Kezi̇ban Yazici, Mehtap Şahi̇n Çevi̇k

Turkish Journal of Agriculture and Forestry

Production and consumption of pomegranate have been increasing in the world in recent years due to its health benefits to humans. Sunburn is an important physiological disorder caused by high temperature, light, and radiation. With the changes in climatic conditions that have occurred in recent years, the effect of sunburn is increasing and limiting the production and quality of pomegranate. Therefore, development of pomegranate cultivars resistance to sunburn is one of the main objectives of pomegranate breeding program. However, the development of a new variety takes a very long time. In order to shorten this time, molecular markers have been …

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Biochar Mitigates Salt Stress By Regulating Nutrient Uptake And Antioxidant Activity, Alleviating The Oxidative Stress And Abscisic Acid Content In Cabbage Seedlings, Melek Eki̇nci̇, Meti̇n Turan, Ertan Yildirim Jan 2022

Biochar Mitigates Salt Stress By Regulating Nutrient Uptake And Antioxidant Activity, Alleviating The Oxidative Stress And Abscisic Acid Content In Cabbage Seedlings, Melek Eki̇nci̇, Meti̇n Turan, Ertan Yildirim

Turkish Journal of Agriculture and Forestry

A pot experiment was conducted to investigate the growth, physiology, and biochemistry of cabbage seedlings in response to salinity stress with biochar. The study was conducted as factorial completely randomized design with two factors; salinity levels (0 and 150 mM NaCl) and biochar doses (weighed at the rate of 0%, 2.5%, and 5% by soil weight). Growth parameters such as stem diameter, leaf area, shoot fresh weight, root fresh weight, shoot dry weight, and root dry weight of cabbage seedlings treated with 150 mM NaCl were lower than the control. Biochar amendments improved the growth parameters of cabbage seedlings under …

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Prunus Microcarpa: A Potential Rootstock For Apricots, Remzi̇ Uğur Jan 2022

Prunus Microcarpa: A Potential Rootstock For Apricots, Remzi̇ Uğur

Turkish Journal of Agriculture and Forestry

One of the most important developments in the history of fruit growing is the discovery and use of dwarf rootstocks. Today, dwarf rootstock studies continue in different fruit species including apricot. The main goal of this study was to identify new dwarfing rootstocks for apricots that limit apricot tree height and size, without altering scion production or fruit characteristics. In this study, `Hacıhaliloğlu', `Hasanbey', `Kabaaşı', and `Roxana' apricot cultivars grafted on Prunus microcarpa - a potential rootstock for apricot - were investigated. Phenological, morphological, yield, and quality traits of apricot (Prunus armeniaca L.) cultivars grafted on Prunus microcarpa seedlings and …

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Cadmium Tolerance And Its Absorption Ability In Fibre Flax And Linseed Varieties, Ekaterina Brutch, Olga Zabegaeva, Janka Nozkova, Nina Brutch Jan 2022

Cadmium Tolerance And Its Absorption Ability In Fibre Flax And Linseed Varieties, Ekaterina Brutch, Olga Zabegaeva, Janka Nozkova, Nina Brutch

Turkish Journal of Agriculture and Forestry

Cadmium pollution now is one of the main environmental problems. Growing of plants hyper accumulators of Cd can help to remediate the polluted soils. Species Linum usitatissimum L. is one of such crops. Six fibre flax and two linseed varieties were evaluated for their resistance to different cadmium sulfate 8/3-hydrate 3CdSO4 . 8H2O concentrations at the seedlings stage in the Jacobsen apparatus. After this, two fibre flax and two linseed varieties were selected and tested for adult plants' cadmium resistance and absorption ability. Cadmium concentrations were analysed in the ashes of adult plants by x-ray fluorescence analyzer BRA - 18. …

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Genome-Wide Association Studies (Gwas) Revealed A Genetic Basis Associated With Floral Traits In Potato Germplasm, Muhammad Abu Bakar Zia, Ufuk Demi̇rel, Muhammad Azhar Nadeem, Fawad Ali, Ahmad Dawood, Muhammad Ijaz, Mehmet Emi̇n Çalişkan Jan 2022

Genome-Wide Association Studies (Gwas) Revealed A Genetic Basis Associated With Floral Traits In Potato Germplasm, Muhammad Abu Bakar Zia, Ufuk Demi̇rel, Muhammad Azhar Nadeem, Fawad Ali, Ahmad Dawood, Muhammad Ijaz, Mehmet Emi̇n Çalişkan

Turkish Journal of Agriculture and Forestry

Potato is an important noncereal staple crop serving as a source of food for a large number of the world?s population. Genomewide association study (GWAS) analysis has become a useful tool to uncover the genetic basis of important plant traits by revealing significant association with the trait of interest. The present study aimed to explore the phenotypic diversity and to identify the genetic basis associated with important floral traits. A total of 237 tetraploid potato genotypes were used as plant material, and field experiments were conducted according to augmented block design for two consecutive years (2016, 2017). Analysis of variance …

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Unlocking The Genomic Regions Associated With Seed Protein Contents In Turkishcommon Bean Germplasm Through Genome-Wide Association Study, Faheem Shahzad Baloch, Muhammad Azhar Nadeem Jan 2022

Unlocking The Genomic Regions Associated With Seed Protein Contents In Turkishcommon Bean Germplasm Through Genome-Wide Association Study, Faheem Shahzad Baloch, Muhammad Azhar Nadeem

Turkish Journal of Agriculture and Forestry

Human body needs sufficient quantity of protein on daily basis for the normal functioning. Dietary protein in sufficient quantity is becoming a key issue for huge population of the world particularly in developing countries. Present investigation aimed to explore the phenotypic variation and to investigate the genomic regions associated with seed protein contents in Turkish common bean germplasm collected from 19 provinces. Field experiments were conducted according to augmented block design at two locations (Bolu and Sivas) for two consecutive years (2017 and 2018). Analysis of variance revealed highly significant environmental effects (p < 0.05) on seed protein contents within environments, while genotype by environment interaction revealed nonsignificant effects. Overall mean protein contents were 36.36%, while Balikesir-3 and Hakkari-38 yielded minimum (31.64%) and maximum (40.70%) protein contents. Among 19 provinces, accessions from Hakkari and Erzincan provinces disclosed maximum and minimum mean protein contents respectively. Scatter plot between seed protein contents and plant height divided the accessions according to their protein contents and plant height. A total of eight stable accessions were selected and can be recommended for future breeding activities. The implemented constellation plot separated the studied germplasm into two populations on the basis of their protein contents. Genotyping by sequencing resulted 7900 DArTseq markers was used for marker-trait association and a total of 11 markers showed significant association for protein contents. During this study, DArT-3365703 marker was identified in both locations (Sivas and Bolu) during 2017 and might be used for future common bean breeding. Physical map revealed the presence narrow regions between identified markers on Pv02 and Pv05. Therefore, these regions should be considered for future breeding activities. We are confident that results of present study will be helpful for marker-assisted breeding of common bean.

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Research On The Potential Of Some Sweet Sorghum Genotypes As Bioethanol Source Undermediterranean Conditions, Celal Yücel, Derya Yücel, Rüştü Hati̇poğlu, Ismail Dweikat Jan 2022

Research On The Potential Of Some Sweet Sorghum Genotypes As Bioethanol Source Undermediterranean Conditions, Celal Yücel, Derya Yücel, Rüştü Hati̇poğlu, Ismail Dweikat

Turkish Journal of Agriculture and Forestry

Sweet sorghum (Sorghum bicolor var. saccharatum (L.) Mohlenbr.) is a variety of sorghum developed for the harvest of sweet juice, rather than grain. It has been identified as a potential ethanol feedstock crop for rainfed areas in different parts of the world. It is a promising source of bioenergy due to its high biomass, drought tolerance and low input. This study was conducted to determine the potential of bioethanol production from different sweet sorghum varieties under the Çukurova conditions with a Mediterranean climate. Experiments were conducted over two years in 2016 and 2017 on the experimental fields of the Eastern …

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Induction Of Polyploidy In Grapevine (Vitis Vinifera L.) Seedlings By In Vivo Colchicineapplications, Zeki̇ Kara, Kevser Yazar Jan 2022

Induction Of Polyploidy In Grapevine (Vitis Vinifera L.) Seedlings By In Vivo Colchicineapplications, Zeki̇ Kara, Kevser Yazar

Turkish Journal of Agriculture and Forestry

Polyploidization is an important technique used in grape breeding to create new genetic resources and can be induced using some antimitotic agents, including colchicine. Plants with increased ploidy can gain better quality than their original diploids in some characteristics. In this study, which was carried out to obtain polyploid genotypes under in vivo conditions, the effects of six (1 g L-1, 2 g L-1, 3 g L-1, 4 g L-1, 5 g L-1, 6 g L-1) doses of colchicine were applied to the shoot tip meristem regions of the seedlings obtained from the seeds of grapevine cv. Ekşi Kara and …

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Medicinal And Aromatic Plants In The Omics Era: Application Of Plant Breeding Andbiotechnology For Plant Secondary Metabolite Production, Muzaffer Barut, Muhammad Azhar Nadeem, Özlem Akgür, Leyla Sezen Tansi, Muhammad Aasim, Muhammad Tanveer Altaf, Faheem Shahzad Baloch Jan 2022

Medicinal And Aromatic Plants In The Omics Era: Application Of Plant Breeding Andbiotechnology For Plant Secondary Metabolite Production, Muzaffer Barut, Muhammad Azhar Nadeem, Özlem Akgür, Leyla Sezen Tansi, Muhammad Aasim, Muhammad Tanveer Altaf, Faheem Shahzad Baloch

Turkish Journal of Agriculture and Forestry

Human being has strong and historic connections with plants to fulfill food, feed, and shelter. Moreover, human uses plants as medicine for the treatment of various diseases. Plants are chemical factories for the biosynthesis of the huge number of secondary metabolites (SMs) that are directly used as a medicine or indirectly used in the development of commercial pharmaceutical drugs. Their improvement through classical breeding remains a difficult struggle for plant breeders. Hence, rapidly advancing research on the plant omics era has accelerated our understanding of the complex structure of SMs synthesis in medicinal and aromatic plants (MAPs). In addition, sequencing …

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Morphological, Biochemical, And Bioactive Characterization Of Naturally Growneuropean Cranberrybush Genotypes, Ayşen Melda Çolak, Fatma Alan, Kerem Mertoğlu, İbrahi̇m Bulduk Jan 2022

Morphological, Biochemical, And Bioactive Characterization Of Naturally Growneuropean Cranberrybush Genotypes, Ayşen Melda Çolak, Fatma Alan, Kerem Mertoğlu, İbrahi̇m Bulduk

Turkish Journal of Agriculture and Forestry

Turkey has very rich potential in terms of wild edible fruit species, especially berries that grow naturally in different parts of the country as population or scattered shrubs. One of the most important wild edible fruits found in Turkey's flora is the European cranberrybush (EC). All EC plants are found as wild in nature and there are no commercial EC orchards in the country. In this study, a total of 22 seed propagated EC genotypes that naturally grown in the Akkışla district of Kayseri province located in the middle part of Turkey were assessed in terms of their morphological (fruit …

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Inter-Primer Binding Site (Ipbs) Retrotransposon Markers Provide Insights Into Thegenetic Diversity And Population Structure Of Carrots (Daucus, Apiaceae), Mehtap Yildiz, Carlos Arbizu Jan 2022

Inter-Primer Binding Site (Ipbs) Retrotransposon Markers Provide Insights Into Thegenetic Diversity And Population Structure Of Carrots (Daucus, Apiaceae), Mehtap Yildiz, Carlos Arbizu

Turkish Journal of Agriculture and Forestry

Studies of genetic diversity and population structure are essential as an initial step in conservation and breeding programs for modern crops. Carrot (Daucus spp.) is among the ten most important vegetables worldwide, however, its genetic structure and phylogenetic relationships are not totally deciphered. Here, we explored the utility of 21 inter-primer binding site (iPBS) retrotransposon markers to determine the genetic diversity and population structure of 38 accessions of Daucus and one accession of a related genus. The manual scoring revealed 309 bands based on their presence/absence. The dendrogram based on the UPGMA clustering algorithm and a principal coordinate analysis (PCoA) …

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Characterization Of Some Local Pea (Pisum Sativum L.) Genotypes For Agro-Morphologicaltraits And Mineral Concentrations, Aybegün Ton, Dürdane Mart, Tolga Karaköy, Meltem Türkeri̇, Ayfer Alkan Torun, Adem Emi̇n Anlarsal Jan 2022

Characterization Of Some Local Pea (Pisum Sativum L.) Genotypes For Agro-Morphologicaltraits And Mineral Concentrations, Aybegün Ton, Dürdane Mart, Tolga Karaköy, Meltem Türkeri̇, Ayfer Alkan Torun, Adem Emi̇n Anlarsal

Turkish Journal of Agriculture and Forestry

The aim of the present study is to evaluate diversity among the Turkish local pea germplasm for mineral concentrations and agro-morphological traits for breeding programs. The experiment was conducted during 2018/2019 and 2019/2020 and arranged according to an augmented block design with 40 local genotypes and two standard check cultivars (Carina and Jof). Seed yield per plant was positively and significantly correlated with branches per plant, pods per plant, seeds per pod, harvest index, and 100-seed weight. The first three principal components (PC1) for agro-morphological traits accounted for 76.3% of the total variation. The principal first component was positively correlated …

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Cover And Contents Jan 2022

Cover And Contents

Turkish Journal of Agriculture and Forestry

No abstract provided.

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Study Of Anatomical And Acc (1-Aminocyclopropane-1-Carboxylate) During Flower Budabscission Indicate An Important Role Of Alternate Bearing, Muhammet Ali̇ Gündeşli̇, Sali̇h Kafkas, Şenay Karabiyik, Si̇nan Eti̇, Nesi̇be Ebru Kafkas Jan 2022

Study Of Anatomical And Acc (1-Aminocyclopropane-1-Carboxylate) During Flower Budabscission Indicate An Important Role Of Alternate Bearing, Muhammet Ali̇ Gündeşli̇, Sali̇h Kafkas, Şenay Karabiyik, Si̇nan Eti̇, Nesi̇be Ebru Kafkas

Turkish Journal of Agriculture and Forestry

This study investigates whether, and how, 1-aminocyclopropane-1-carboxylic acid (ACC) is concerned in flower bud abscission of pistachio (Pistacia vera L.). Abscission was studied about the activation of the abscission zone, located between shoot and flower bud, from Uzun pistachio cultivar where the abscission profiles and the scanning electron micrographs pictorial differences in the abscission program, under natural conditions of flower bud. ACC content was investigated in the flower bud abscission zone during growth and abscission using liquid chromatography coupled with electrospray tandem mass spectrometry (UPLC/ESI-MS/MS). Results showed that abscission layers were started at first 45 days after full blooming (DAFB) …

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The Effects Of Zinc Oxide Nanoparticles Loaded With Iaa And Iba On In Vitro Rooting Ofapple Microcuttings, Shabnam Alizadeh, Hati̇ce Dumanoğlu Jan 2022

The Effects Of Zinc Oxide Nanoparticles Loaded With Iaa And Iba On In Vitro Rooting Ofapple Microcuttings, Shabnam Alizadeh, Hati̇ce Dumanoğlu

Turkish Journal of Agriculture and Forestry

In the micropropagation of difficult-to-root plants, the low rates of rooting cause high economic losses. The local apple (Malus domestica Borkh.) cultivar #67, which is highly resistant to fire blight disease, has been shown to have low rooting success. Nanoparticles loaded with auxins could increase the rooting success with their physicochemical properties. To increase rooting percentage, the effects of zinc oxide nanoparticles (nZnO), nZnO loaded with indole-3-acetic acid (IAA-nZnO) and indole-3-butyric acid (IBA-nZnO) at different concentrations (1.0, 2.0, 3.0, 4.0, 5.0, and 6.0 mg L-1) on in vitro rooting of microcuttings were investigated. The concentrations of auxin carried by the …

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Comparative Study Of Phenolic Compounds In Olive Oils From Differentgeographic Regions, Şeyma Şi̇şi̇k Oğraş Jan 2022

Comparative Study Of Phenolic Compounds In Olive Oils From Differentgeographic Regions, Şeyma Şi̇şi̇k Oğraş

Turkish Journal of Agriculture and Forestry

In this study, the effect of cultivar, harvest year, and the geographic regions (Mediterranean, Aegean, Southeastern Anatolia, Marmara, and the Black Sea) on phenolic compounds of virgin olive oils were investigated. Tyrosol was the major phenolic compound in the Mediterranean, Southeastern, and Black Sea regions. Hydroxytyrosol showed the highest value (2.07 mg/kg) in the Southeastern Anatolia region and the lowest value (0.75 mg/kg) in the Marmara region. Among the phenolic acids, vanillic acid had an important place in phenolic compounds (p < 0.01). This phenolic acid gave higher values in the Marmara region compared to other regions. Flavonoids were determined as important groups of phenolic compounds in olive oils. The oils from the Southeastern Anatolia region separated from other oils with the highest levels of flavonoids such as quercetin, luteolin, and apigenin. All phenolic compounds, except transcinnamic acid and quercetin, increased in the second harvest year. Vanillic acid was the highest phenolic compound in the first year, in contrast, tyrosol had higher values than vanillic acid in the second year. Many phenolic compounds were affected by olive cultivar. Memecik has a higher content of tyrosol, vanillic acid, p-coumaric acid, ferulic acid, and quercetin in the Aegean region. However, this variety showed higher values for hydroxytyrosol, transcinnamic acid, luteolin, and apigenin in the Marmara region. Gemlik, Ayvalık, Çelebi, Domat and Memecik from Marmara regions had higher vanillic acid. Butko, Otur and Kızıl Satı showed higher tyrosol. Principal component analysis showed that phenolic compounds could play an important role in the separation of regions and cultivars.

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Effect Of Bursaphelenchus Spp. Inoculation On Carbohydrate Concentrations Of Differentpine Species In Forest Stands Of Düzce Forest Directorate, Nuray Öztürk, Süleyman Akbulut, İsmai̇l Baysal, Şemsetti̇n Kulaç Jan 2022

Effect Of Bursaphelenchus Spp. Inoculation On Carbohydrate Concentrations Of Differentpine Species In Forest Stands Of Düzce Forest Directorate, Nuray Öztürk, Süleyman Akbulut, İsmai̇l Baysal, Şemsetti̇n Kulaç

Turkish Journal of Agriculture and Forestry

The pinewood nematode (Bursaphelenchus xylophilus) is one of the most important pathogens of conifer forests worldwide that causes the pine wilt disease. This problem has increased the scientific interest in Bursaphelenchus species both in the world and in Turkey. Previously, six Bursaphelenchus species were isolated from wilted pine trees in Turkey. The main goal of this study was to investigate the effects of nematode inoculation on the total carbohydrate concentrations of tree species. Native six Bursaphelenchus species were inoculated on three pine species (Pinus pinaster, P. nigra, and P. sylvestris) under natural stand conditions. Two different doses of nematodes (2000 …

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An Examination Of The Impact Of Covid-19 On Assessment Practices In Higher Education, Ziene Mottiar, Greg Byrne, Geraldine Gorham, Emma Robinson Jan 2022

An Examination Of The Impact Of Covid-19 On Assessment Practices In Higher Education, Ziene Mottiar, Greg Byrne, Geraldine Gorham, Emma Robinson

Articles

The COVID-19 pandemic resulted in a rapid pivot to online learning across many higher education institutions globally. This paper investigates to what extent assessment strategies changed as a result of this pivot. It explores the case of Technological University Dublin (TU Dublin) in Ireland and finds that 95% of respondents altered their assessment practices in some way. Beyond identifying changing practice, the paper also develops a TARC (Typology of Assessment Responses to COVID-19) model which shows four categories of responses. Reactors are those academics who simply moved their assessments online. Adaptive Responders modified assessments slightly for the online environment. While …

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Rhetoric Of Surrogacy: Re-Considering Agency Through Embodied Performance, Ann Kitalong-Will Jan 2022

Rhetoric Of Surrogacy: Re-Considering Agency Through Embodied Performance, Ann Kitalong-Will

Dissertations, Master's Theses and Master's Reports

Surrogacy as a medical practice goes back, in a practical sense, to 1988, when the court case, “In the Matter of Baby M, A Pseudonym for an Actual Person,” was tried in the Supreme Court of New Jersey. At the heart of the issue, was the question of who Baby M’s legally-recognized mother was in the relationship between the contracting parents and the woman who gestated and gave birth to Baby M. Using this case as a jumping off point, this dissertation traces a history of surrogacy as a global industry. This project explores rhetorical agency in the embodied performance …

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Towards A Circular Economy: Liquid-Fed Fast Pyrolysis Of Waste Polyolefin Plastics, Daniel G. Kulas Jan 2022

Towards A Circular Economy: Liquid-Fed Fast Pyrolysis Of Waste Polyolefin Plastics, Daniel G. Kulas

Dissertations, Master's Theses and Master's Reports

Plastic usage has quickly grown in recent years as plastics have become an essential part of everyday life. Unfortunately, recycling rates have not matched the increased growth, with the majority of waste plastic landfilled. The strategy of circular economy has been proposed to reuse the waste plastic to create new polymers, thus avoiding both landfilling and the use of virgin raw materials. Achieving a circular economy for plastics requires the development of new technologies capable of recycling plastic waste under a circular economy perspective. In this dissertation, a novel liquid-fed fast pyrolysis process is introduced towards achieving a circular economy. …

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Collective Hydrodynamics Of Robotic Fish, Rohit S. Pandhare Jan 2022

Collective Hydrodynamics Of Robotic Fish, Rohit S. Pandhare

Dissertations, Master's Theses and Master's Reports

Many animals in nature travel in groups either for protection, survival, or endurance. Among these, fish do so under the burden of hydrodynamic loads, which incites questions as to the significance of the multi-body fluid-mediated interactions that facilitate collective swimming. We study such interactions in the idealized setting of a rotational array of robotic fish whose tails undergo a prescribed flapping motion, but whose swimming speed is determined as a natural result of the hydrodynamic effects. Specifically, we examine how the measured collective speed of the swimmers varies with the imposed frequency and amplitude of their tail flapping, and with …

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Characterizing Drought Adaptations, Phenotypic Plasticity, And Fixed Gene Expression Patterns Within Quercus, James K. Rauschendorfer Jan 2022

Characterizing Drought Adaptations, Phenotypic Plasticity, And Fixed Gene Expression Patterns Within Quercus, James K. Rauschendorfer

Dissertations, Master's Theses and Master's Reports

This dissertation was written on topics related to the genus Quercus with a primary focus on Quercus ellipsoidalis (northern pin oak) and Quercus rubra (northern red oak). Within this dissertation are chapters related to the setup of experimental common gardens within the Ford and Kellogg experimental forest, a literature review describing drought adaptations of Quercus sect. Lobatae (red oak group), identification of transcription factors within the Q. robur (English oak) and Q. rubra genomes, a study comparing leaf trait phenotypic plasticity of Q. ellipsoidalis and Q. rubra, and an RNA-seq experiment studying ecological speciation between Q. ellipsoidalis and Q. …

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Avian Haemosporidian Blood Parasite Diversity, Prevalence, And Distribution In Michigan’S Western Upper Peninsula, Maria M. Ferrer Jan 2022

Avian Haemosporidian Blood Parasite Diversity, Prevalence, And Distribution In Michigan’S Western Upper Peninsula, Maria M. Ferrer

Dissertations, Master's Theses and Master's Reports

Avian haemosporidian parasites, known as avian malaria (phylum Apicomplexa) can diminish an individual bird’s fitness by causing parasitemia, anemia, and reduced survival. Climate change is predicted to increase the spread of malarial parasites into more northerly latitudes where little is known about community compositions of these parasites. I assessed the prevalence and diversity of haemosporidian parasites in the first-ever community-level sampling of malaria in songbirds across the western Upper Peninsula of Michigan. In total, 179 blood samples were collected from birds representing 17 species, at five locations in the Upper Peninsula, including a mature forest, an early successional forest, and …

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Multilevel Computational Investigation Into The Dynamics And Reaction Mechanisms Of Non-Heme Iron And 2-Oxoglutarate Dependent Enzymes, Shobhit Sanjeev Chaturvedi Jan 2022

Multilevel Computational Investigation Into The Dynamics And Reaction Mechanisms Of Non-Heme Iron And 2-Oxoglutarate Dependent Enzymes, Shobhit Sanjeev Chaturvedi

Dissertations, Master's Theses and Master's Reports

Computational chemistry methods have been extensively applied to investigate biological systems. This dissertation utilizes a multilevel computational approach to explore the dynamics and reaction mechanisms of two groups of enzymes belonging to non-heme Fe(II) and 2-oxoglutarate (2OG) dependent superfamily – histone lysine demethylases from class 7 and ethylene forming enzyme (EFE). Chapter 2 uncovers the role of conformational dynamics in the substrate selectivity of histone lysine demethylases 7A and 7B. The molecular dynamics (MD) simulations of the two enzymes revealed the importance of linker flexibility and dynamics in relative orientations of the reader (PHD) and the catalytic (JmjC) domains. Chapter …

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Isolation And Chromatographic Separation Of Cytotoxic Plant Compounds, Michael C. Hromada Jan 2022

Isolation And Chromatographic Separation Of Cytotoxic Plant Compounds, Michael C. Hromada

Dissertations, Master's Theses and Master's Reports

Plants produce diverse bioactive compounds that are used in the treatment of diseases such as cancer, microbial infection, and cardiovascular diseases. Our lab has discovered a potential anticancer compound named Hemolysin X (HelyX) in the storage and germination tissues of a plant species. Investigation of this compound relies on aqueous extraction which limits the isolation of other potentially important hydrophobic compounds. To overcome this limitation and to determine if HelyX is distributed in other tissues of the plant (such as flower), organic extracts of flower tissue were examined by analytical thin layer chromatography (TLC), preparative TLC, and silica gel column …

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Comparative Transcriptomic Analysis Of Cancer Testis Genes In Ovarian Cancer, Zayne Knuth Jan 2022

Comparative Transcriptomic Analysis Of Cancer Testis Genes In Ovarian Cancer, Zayne Knuth

Dissertations, Master's Theses and Master's Reports

Cancer testis genes are common targets for the development of immunotherapy for cancer treatment. Ovarian cancer is one of the leading causes of death in women cancer patients. Cancer testis genes play a role in tumorigenesis, but it is not clear how these genes are activated. This study utilized differential expression analysis between The Cancer Genome Atlas (TCGA) ovarian cancer data, Genotype-Tissue Expression (GTEx) non-cancerous ovary and testis data, and cell line data to identify a list of cancer testis genes that have a novel expression profile. To identify ovarian cancer testis genes, we obtained normal ovary tissue data and …

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An Analysis Of Compressive Convolutional Autoencoders For Image Archiving In Medical Informatics, Charles Warren Jan 2022

An Analysis Of Compressive Convolutional Autoencoders For Image Archiving In Medical Informatics, Charles Warren

Dissertations, Master's Theses and Master's Reports

Within a given enterprise network, an array of data types needs to be communicated. These network transmissions consist of images, videos, text, and binaries that have unique requirements of bandwidth and computational overhead to transmit. With respect to medical informatics, these include a multitude of varying subjects, standards, and modalities which are communicated to and from imaging equipment, clinicians, and medical archives. To reduce the required bandwidth to transmit, or provide adequate storage capacity for archival purposes, the data may be compressed in such a way that reduces the size of the image when it is transferred or stored. The …

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Spatial Heterogeneity Of Nitrogen Cycling Within And Across Freshwater Ecosystems, Erin K. Eberhard Jan 2022

Spatial Heterogeneity Of Nitrogen Cycling Within And Across Freshwater Ecosystems, Erin K. Eberhard

Dissertations, Master's Theses and Master's Reports

Dinitrogen (N2) fixation and denitrification are two nitrogen (N) cycling processes that despite differences in environmental requirements and constraints, co-occur in aquatic ecosystems. The overall goal of this dissertation was to evaluate how spatial heterogeneity of environmental variables 1) drive hot spots of N2 fixation, denitrification rates and gene abundances in streams, 2) facilitate co-occurrence of these processes across wetland – stream – lake interfaces, and 3) affect differences in microbial community composition in streams across U.S. ecoregions. We found hot spots of both processes within 7 stream reaches in Michigan and Idaho, but rates of N …

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Evolutionary Conservation Of The Dream Subcomplex Muvb, Spencer Snider Jan 2022

Evolutionary Conservation Of The Dream Subcomplex Muvb, Spencer Snider

Dissertations, Master's Theses and Master's Reports

As the publications of annotated genomes from species representing most domains of life continue to grow exponentially, we are gaining more insight into how proteins, cellular pathways, and protein complexes evolved. We are interested in understanding how each protein in the 8-subunit transcriptional repressor complex called DREAM interacts with each other. DREAM is comprised of 3 main components: an E2F-DP transcription factor heterodimer, a pocket protein, and the highly conserved 5-subunit subcomplex called MuvB. We hypothesize that the mechanism of DREAM’s formation on chromatin dictates how DREAM functions to turn off target gene expression. Unfortunately, many interaction surfaces remain unknown, …

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