Digital Commons Network^™

Investigation Of Pfas Exposure Risks In Kentucky Using Mapping Tools, Sweta Ojha

Theses and Dissertations--Civil Engineering

Per- and polyfluoroalkyl substances (PFAS), a group of environmentally-persistent compounds, are environmentally ubiquitous and challenging to remediate. Several studies show that PFAS are detected in drinking water systems when sampling is conducted, making human exposure via drinking water an important health consideration. This research: 1) develops a mapping tool for prioritizing sampling locations; 2) establishes a method for making GIS data and meta(data) in the mapping tool accessible; 3) fosters decision making by integrating knowledge brokering and the alignment interest and influence matrix (AIIM). The tool developed is this research is a geospatial and statistical PFAS hot-spot screening model that …

Knitting Code: Examining The Relationship Between Knitting And Computational Thinking Skills Using The Nexus Of Practice, Emily Rose Dodson-Snowden

Theses and Dissertations--Education Sciences

Due to the rise of careers in STEM-related fields, there is a growing need for schools to produce people to fill these positions. One area of STEM that is growing is computer science/coding. Due to this demand, schools need to be intentional about exposing students to computer science/coding. There are a variety of new tools to introduce students to this field. One growing belief is that knitting can teach computer science/coding to students.

The goal of this study was to see if knitting can serve as an introduction to teach students computation skills. Kitting has historically been used to code …

How Does Exposure To An Authentic English-Speaking Environment Contribute To Chinese International Students’ Academic Reading Comprehension?, Na Liu

Theses and Dissertations--Education Sciences

Chinese international students comprise the largest group of international students in U.S. universities. To better support international students to be successful in international higher education, Chinese students’ reading comprehension in English deserves attention. Using a qualitative research design, this study analyzed Chinese international students’ perceptions about their English reading comprehension in academic literacy, and how exposure to an English-speaking environment contributed to their reading comprehension development. There were 8 participants involved who have different genders (males and females), education levels (undergraduates and graduates), exposure time (less than one year and more than one year), and majors (chemistry, engineering, arts, etc.). …

Development Of The Elementary Teachers’ Commitment To Outdoor Education Survey: A Mixed Methods Study, Sagan Goodpaster

Theses and Dissertations--Education Sciences

As the Green Schoolyards Movement continues to grow throughout North America, outdoor education (OE) is gaining more attention in the formal K-12 curriculum (Dyment, 2005; Sterrett & Imig, 2015). Rooted in the pedagogies of place-based and experiential learning, outdoor instruction provides social, psychological, physical, and academic benefits for students, and fosters their sense of connection to the natural environment (Dubel & Sobel, 2008; Robertson, 2017). In particular, outdoor learning opportunities have been shown to promote greater gains in students’ scientific content knowledge (Carrier et al., 2014; Cronin-Jones, 2000; Dirks & Orvis, 2005; Kenney et al., 2003). Outdoor instruction involves students …

(Dis)Possessed Black Youth: How America's Architecture Challenges Coming Of Age In Twentieth And Twenty-First Century African American Women's Literature, Margaret Frymire Kelly

Theses and Dissertations--English

This dissertation advances studies of Black childhood, particularly Black girlhood, by examining how African American women writers depict the troubled journey to adulthood in stories of segregation, immigration, and incarceration. I argue that authors of four representative literary works emphasize architectural structures as well as ancestral hauntings among which Black children grow up. Without examining the material structures, we cannot understand the strategies these haunted Black youth deploy to reach adulthood. Examining the architectural structures that the protagonists of Maud Martha (1953), Brown Girl, Brownstones (1959), Zami (1982), and Sing, Unburied, Sing (2017) grow up in and around, I demonstrate …

Investigating The Role Of Chd7 And Sox11 In Retinal Cell Development And The Ocular Complications Of Charge Syndrome, Laura Krueger

Theses and Dissertations--Biology

Proper formation of the visual system requires the precise interaction of several embryonic cell lineages, including the neuroectoderm (forms the retina and retinal pigment epithelium), surface ectoderm (forms the lens), mesoderm and cranial neural crest cells (form the ocular blood vessels and anterior ocular structures). When this process is disrupted structural birth defects such as coloboma result, leading to pediatric visual deficits. Ocular developmental defects are often present in larger syndromic disorders. One example is CHARGE syndrome, a genetic disorder characterized by coloboma, heart defects, choanal atresia, growth retardation, genital abnormalities, and ear abnormalities. Pathogenic variants in CHD7 have been …

Metabolic Diversity Among Fibroblasts From Regenerating And Non-Regenerating Mammals, Ebenezer Aryee

Theses and Dissertations--Biology

Regeneration is a wound repair process that terminates in the restoration of tissue function and structure. Fibroblasts play pivotal roles in regenerative and fibrotic wound repair. Reports of extensive regenerative ability in mammals have been historically rare, but more recently spiny mice (Acomys) have emerged as a bona fide model of complex tissue regeneration. Recent work has indicated that fibroblasts from regenerators (Acomys and Oryctolagus) are more resistant to reactive oxygen species (ROS)-induced senescence compared to non-regenerating species, suggesting the influence of intrinsic cellular states on the fate of wound repair. Determining the basal metabolic signature …

The Ecology And Evolution Of Human Reproductive Behavior, Kaylynne Glover

Theses and Dissertations--Biology

The complexity of human reproductive behavior has necessitated its examination through a variety of scientific disciplines, each focusing on specific elements of our biology, behavior, and society. However, this complexity also necessitates that we reintegrate the information learned from each discipline into a single framework, one rooted in the evolutionary principles that have shaped the development of all life on earth. In this dissertation, I use this framework to explore human reproductive behavior, with a particular focus on sexual coercion and fertility-mediated sexual behavior.

In Chapter 1, I introduce the approach taken in this document, identify several key limitations, and …

Estrogen Regulation Of Daily Metabolic Rhythms In Female Mice, Oluwabukola Omotola

Theses and Dissertations--Biology

Circadian rhythms are approximately 24-hour cycles of behavior, physiology, and gene expression. In mammals, these circadian rhythms are generated by clocks located in nearly every tissue in the body. The function of circadian clocks is to synchronize physiology and behavior with environmental cycles such as the light-dark cycle. After menopause, when circulating levels of estrogens are very low, women are more susceptible to obesity comorbidities such as metabolic syndrome and cardiovascular disease, suggesting that estrogens regulate these processes. Estrogens could protect females from metabolic dysfunction by regulating circadian rhythms. Consumption of diets that are high in fat contribute to obesity. …

Distribution Of Particulate Organic Carbon – Fluxes And Inventories – In Growth-Faulted Coastal Wetlands, Samuel Joel Whitehead

Theses and Dissertations--Earth and Environmental Sciences

Coastal wetlands along the Northern Gulf of Mexico (NGOMx) are critically important but threatened environments which provide significant and diverse economic, social, and environmental value. These environments are essential components to the global carbon cycle, serving as one of the most efficient terrestrial carbon sinks. Since onset of the Industrial Revolution increasing rates of coastal wetland loss have been documented due to a variety of anthropogenic activities, neotectonic processes, subsidence, and rising sea-levels. This research utilized an array of methods and sedimentological analyses to test the hypothesis that growth-fault induced changes at Earth’s surface affects the delivery to, and storage …

Tryptophan-Like Fluorescence And Non-Point Source Pollution In Karst Basins, Inner Bluegrass Region, Kentucky, Ryan Dapkus

Theses and Dissertations--Earth and Environmental Sciences

Typically, the degree of fecal contamination of water is assessed by the concentration of thermotolerant coliforms, like E. coli. This method is time-consuming, taking > 18 hours between the start of incubation and subsequent enumeration. The current study examines the utility of monitoring tryptophan-like fluorescence (TLF) using in-situ fluorometers as a proxy for fecal contamination, particularly E. coli. Relative timing of discharge peaks and TLF peaks is considered, as well as differences in E. coli concentrations and major anion concentrations between two different basins that were monitored weekly. Results indicate that TLF is somewhat useful in the prediction of …

The Role Of Sox4 In Ocular Morphogenesis And Retinal Differentiation, Rebecca Petersen

Theses and Dissertations--Biology

Visual impairment ranges from mild forms that can be corrected with glasses to more severe cases that result in permanent loss of vision. Microphthalmia, anophthalmia, and coloboma (collectively referred to as MAC) account for 11% of cases of pediatric blindness and are a result of improper ocular morphogenesis. Retinitis Pigmentosa (RP) is a retinal degenerative disease that affects 1 in 3000 people worldwide. It is a progressive disorder that initially begins with loss of vision in low light settings due to rod photoreceptor degeneration but progresses to complete blindness upon loss of cone photoreceptors. Currently, there is no cure for …

Undoing Colorblind Ecologies: Redlining And Just Green Enough In The Urban Forest Of Boston's Franklin Park, Chelsea M. Parise

Theses and Dissertations--Geography

Urban political ecology research increasingly engages multi-disciplinary methodologies to clarify the role that the botanic plays in creating, maintaining, or subverting ecological geographies of power. Fredrick Law Olmsted intended the forest within Franklin Park to heal the physical degeneration and social disunity he believed resulted from urban living conditions but instead the forest within Franklin Park has grown in contexts of increasingly complex environmental and racial difference. I examine how the urban forest in Boston’s Franklin Park has ecologically manifested racialized power relations through distinct periods of elite nature-making and segregated grassroots stewardship. I utilized archival research, forest surveys, and …

Elephants In The Room: Covid-19 Pandemic Political Ecologies Of Tourism In Tanzania, Helen C. Richardson

Theses and Dissertations--Geography

The COVID-19 pandemic brought forth unprecedented and ever-changing crisis and disruption to societies and economies around the globe.[1] As the COVID-19 pandemic continues to interrupt travel worldwide, the tourism industry, and the countries who rely on it as a major source of income, are in crisis. These processes have reconfigured economic capital flows and foreign investment in the global south. This is particularly the case in Tanzania, as tourism was Tanzania’s highest foreign exchange earner and accounted for 17% of Tanzania’s gross domestic product in 2019.[2] This project draws upon a political ecology framework to examine the Tanzanian …

Speaking Of Home Here And There: Everyday Experiences Of Belonging Among Highly Educated Immigrants, Katherine Feske-Kirby

Theses and Dissertations--Geography

This thesis explores how highly educated immigrants articulate a sense of belonging upon relocating to the United States, more specifically to the Lexington, KY area. Engaging with feminist political geography as well as migration and cultural studies, I argue that articulations of belonging are framed through transnational attachments, which respectively expand individuals’ ability to employ everyday forms of belonging. Expressions and understandings of transnational belonging are framed through in-depth interviews on participants’ workplace, relational dynamics, and engagement with the geopolitical discourse on migration. Through these interviews, a broader representation belonging is presented, while questions on highly educated immigrants’ privilege and …

Mapping Memory: Locational Memory In The First-Person Narrative Of Three Latinx Writers, Stephanie R. Beasley

Theses and Dissertations--Hispanic Studies

Locational memory, which relies upon our natural inclination to store and recall images, adds spatial orientation to a narrative, and provides an accessible framework for the recreation of the past in first-person narrative. The power of locational imagery as a device of memory is both historically and scientifically supported. It is essential to the system of artificial memory that the ancient Greeks called a memory palace, described by both Mary Carruthers and Paul Ricouer. Scientifically, studies show that the strongest autobiographical memories are based on visual imagery and that recall of specific locations provides a cognitive basis for the recreation …

Colonial Contraception: American Birth Control Advocates And Their Work In Appalachia, Puerto Rico, And India; 1930-1970, Dana Johnson

Theses and Dissertations--History

By the beginning of the twentieth century, the development of better contraceptives and changing cultural attitudes led to an increased interest in contraceptive research. Although major political, legal, social, religious, and cultural obstacles remained, birth control advocates began to perform clinical trials to identify effective contraceptives and to disseminate contraceptive information. These trials began in the United States, but birth control advocates quickly introduced them into other areas. In this dissertation, I examine the research efforts of the American birth control movement through an analysis of the activities and discourse of its key advocates and promoters during the middle decades …

Batch Normalization Preconditioning For Neural Network Training, Susanna Luisa Gertrude Lange

Theses and Dissertations--Mathematics

Batch normalization (BN) is a popular and ubiquitous method in deep learning that has been shown to decrease training time and improve generalization performance of neural networks. Despite its success, BN is not theoretically well understood. It is not suitable for use with very small mini-batch sizes or online learning. In this work, we propose a new method called Batch Normalization Preconditioning (BNP). Instead of applying normalization explicitly through a batch normalization layer as is done in BN, BNP applies normalization by conditioning the parameter gradients directly during training. This is designed to improve the Hessian matrix of the loss …

The Role Of Sexual Difference In Plato's Timaeus, Mary Cunningham

Theses and Dissertations--Philosophy

My dissertation is a reading of Plato’s Timaeus that centers sexual difference and in particular femininity. I analyze the role of sexual difference in the framing of the dialogue as well as its accounts of body in the first and second discourse and its account of health in the third discourse. I argue that sexual difference, and, in particular, sexual reproduction, serves as a guiding paradigm of Timaeus’ entire project. I argue in each part of my dissertation that various aspects of the Timaeus depend on a certain notion of sexual difference—even aspects which are seemingly causally prior to the …

Significant Life Experiences Of Kentucky Youth Climate Activists, Jeri Katherine Howell

Theses and Dissertations--Community & Leadership Development

The purpose of this study is to better understand Kentucky youth climate activism. The research questions explore how youth define their climate activism in Kentucky, their Significant Life Experiences (SLE) that motivated them to commit to climate activism, and what challenges and sustains their involvement. This qualitative study utilizes a blended framework of social/environmental positionality and political ecology to analyze 7 semi-structured interviews with participants between the ages 18 to 24 years old who are acting to address climate change in Kentucky. New contributions to the existing body of SLE literature are discussed.

Examining Social Mission In Kentucky Social Enterprises, Bo Milburn

Theses and Dissertations--Community & Leadership Development

Social Enterprises are business entities that incorporate social value creation into their business operations. This study aims to investigate, explore and share key insights gained through the lived experience of owners or managers of these organizations. The reputational method of sample selection was used to identify informed individuals who participated in interviews to share phenomenological knowledge by telling the stories of their organizations, describing how they manage the balance between profitability and sustainability, sharing observations about the influence of social mission at their firms and other insights. Participants identified stronger relationships between coworkers, increased professional creativity, and greater employee engagement …

Out Of Sight, Out Of Mind: An Evaluation Of Secondary Agricultural Textbooks, Tara Elizabeth Rojas

Theses and Dissertations--Community & Leadership Development

Students are impacted by representation present within their lives in the media they consume, organizations in which they participate, and school interactions. Among school interactions with primarily White school staff, students interact with textbooks to learn about industry norms and concepts. Missing representation can harm students’ sense of belonging which can lead to feelings like solo-status or isolation. Using both critical race theory and social semiotics, this work explores the representation present within secondary agricultural textbooks. The first study utilizes photos and case studies, observing both gender and race as mutually exclusive variables and compares data to the current public-school …

Membrane Palmitoylated Protein Magu-3 Regulates The C. Elegans Locomotor Circuit Via Modulation Of Cholinergic Activity, Henry Richburg

Theses and Dissertations--Medical Sciences

Precise coordination of the activity and transmission in excitatory and inhibitory neural circuits is essential for healthy information flow. Synaptic scaffolding proteins play a key role in the regulation of circuit activity through the localization and organization of receptors, channels, and other synaptic machinery to facilitate signal transduction. Using the C. elegans motor circuit which has both cholinergic (excitatory) and GABAergic (inhibitory) inputs onto muscles, we revealed an unexplored role of a family of scaffolding molecules known as membrane palmitoylated proteins (MPPs). Here, we examined magu-3, a C. elegans ortholog within the membrane palmitoylated protein family, using GFP tagging …

Mammalian Target Of Rapamycin Cell Signaling Pathway In Phosphatase And Tensin Homolog Induced Kinase 1 Knockout Rat Model Of Familial Parkinson's Disease, Martha Helena Mortell

Theses and Dissertations--Medical Sciences

More than 10 million people are living with Parkinson’s disease (PD), one million of which are people in the United States. PD is the second most common age-related neurodegenerative disorder, after Alzheimer’s disease, and is characterized by the accumulation of a-synuclein aggregates and the degeneration of dopaminergic neurons. The loss of endogenous dopamine in PD brain accounts for the motor decline presented clinically in PD patients. Etiological factors of PD include oxidative damage and inflammation, although the detailed mechanisms remain unknown. Risk factors for PD include gender, age, environmental factors, and gene mutations.

The current thesis research employed phosphatase and …

Apoe Genotype And Sex Modulate Ketogenic Diet Enhancements To Metabolism And Gut Microbiome In Young Mice, Andrew T. Yackzan

Theses and Dissertations--Medical Sciences

The apolipoprotein ε (APOE) allele in humans has been associated with risk for development of Alzheimer’s disease (AD). There are predominately three variations of the allele – ε2 (E2), ε3 (E3), and ε4 (E4) – with E4 contributing the greatest risk of AD development. Recent research has unveiled evidence of neurometabolic and neurovascular deficits in E4 carriers present decades before the onset of dementia; it is believed these chronic defects play a major role in the development of AD, thus making them a potential target for preventative intervention. The purpose of this study was to examine the effect of a …

Evaluating The Role Of Mmp9 In Hyperhomocysteinemia Induced Cerebrovascular Pathology, Alexandria Linton

Theses and Dissertations--Neuroscience

Vascular contributions to cognitive impairment and dementia (VCID) is one of the leading causes of dementia, along with Alzheimer’s disease (AD), and often manifests as a comorbidity of AD. Elevated plasma homocysteine, known as hyperhomocysteinemia (HHcy), is a risk factor for VCID; however, the mechanism underlying the connection between HHcy and development of VCID pathology remains elusive. Understanding this mechanism would reveal novel therapeutic targets with the potential to be disease modifying, which are a critical need for a disease that lacks any approved treatment. Previous studies from our lab have strongly associated neuroinflammation and blood brain barrier (BBB) dysregulation …

Peripheral And Central Glucose Flux In Type I Diabetes, Jelena Anna Juras

Theses and Dissertations--Neuroscience

Diabetes is a complex metabolic disorder, of which high blood glucose concentration is the primary hallmark. Type I diabetes mellitus (T1DM) is characterized by the lack of insulin production, due to a poorly understood autoinflammatory cascade. In the words of historian Barnett “Diabetes may no longer be a death sentence, but for more and more people in the 21st century, it will become a life sentence”, making it the focal point of many research groups. It is estimated that around 20 million individuals worldwide live with T1DM.

Effects of long-term chronically elevated blood glucose are not only seen in micro/macro-vascular …

Interleukin-1 Receptor 1 Signaling In Mild Tbi: Do Endothelial Cells Play A Major Role?, Colleen N. Bodnar

Theses and Dissertations--Neuroscience

Across the world, over 69 million people sustain a traumatic brain injury (TBI) per year making TBI a major health concern worldwide. Of all the TBIs that occur each year, it is suggested that up to 90 percent are mild in nature. Even a mild TBI causes both physical damages to the cells of the brain and activation of a variety of biochemical cascades. Inflammation is an extremely common pathology seen in the brains of TBI survivors of all severities. Chronic inflammation can cause detrimental effects within the brain including neurodegeneration. A major pro-inflammatory cytokine, interleukin-1 (IL-1), is upregulated within …

Conceptualizing Attorney Motivation: A Study Of The Representatives For Parents And Children In The Child Welfare System, Shannon Moody

Theses and Dissertations--Social Work

Background. Attorneys who represent parents and children in dependency, neglect, and abuse (DNA) proceedings are key in influencing the outcomes of the cases they carry. These influences include the length of time a child spends in the custody of the state or the necessity for foster care, visitation with family members, length of time to reunification, and recommended services for the child and parents (Courtney & Hook, 2012; Goldman, 1993; Thornton & Gwin, 2012; Zinn & Peters, 2015). The American Bar Association’s analysis is that there are “four constants: high caseloads, low compensation, inadequate training, and lack of supervision” …

Establishment And Verification Of A Sars Cov2 Antibody Detection Assay Comparing Venous And Mitra Samples, Stephen C. Mayer

Theses and Dissertations--Medical Sciences

During the COVID-19 pandemic in 2020, the need for highly-specific, wide-spread, and rapid serological testing surged. In this study, we showed very strong positive correlation between venous blood collection and Mitra home sampling kits for the detection of anti- spike IgGs using an in-house ELISA protocol (based on a protocol from Mt. Sinai). This study demonstrates the utility of using at-home, patient-centric testing to enhance the sero-surveillance methods currently in place for viral tracking and monitoring.