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Predictions Of Produced Water Quality And Recycled Water Optimization For Spatially-Distributed Wells In Point Pleasant Formation, Armel Quentin Mbakop

Graduate Theses, Dissertations, and Problem Reports (ETD)

The treatment of produced water as a fracturing fluid is becoming an increasingly important aspect of water management surrounding the booming of the unconventional oil and gas industry. Two main problems facing the oil and gas industry are the availability of water for well drilling and completion and disposal of the produced water. Unconventional well drilling and completion in the Utica shale requires large amounts of water. The wastewater that results after production—containing high levels of organic and inorganic matter— is usually disposed of through deep well injection. A new approach reuses this produced water as part of subsequent fracturing …

Summer Habitat Use, Home Range, And Effect Of Catch And Release Mortality On The Trophy Potential Of Muskellunge, (Esox Masquinongy, Mitchill, 1824), In A Southern Reservoir Population, Peter Isaac Jenkins

Graduate Theses, Dissertations, and Problem Reports (ETD)

Research on Muskellunge (Esox masquinongy, Mitchill, 1824) catch-and-release mortality has been historically focused on the Midwest, Great Lakes, and Ontario. In the southern portion of their range, which we consider to be the portion of the species distribution south of the Mason-Dixon Line (~40°N), Muskellunge, may experience significantly warmer temperatures than those in previous studies and anglers have expressed concern about summer catch-and-release mortality. As part of a broader study to quantify catch-and-release summer mortality in the southern range of Muskellunge, we utilized radio telemetry and active tracking to verify individual movement and survival of Muskellunge in Stonewall …

Analyzing The Viability Of Direct Pcr For Use In Conjunction With Cyanoacrylate Enhanced Fingerprints, Coral M. Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Efficient methods for DNA analysis are desperately needed in laboratories due to the influx in DNA analysis requests. The current DNA processing methods are costly, time consuming, and involve multiple tube changes, increasing the risk of contamination. However, the use of direct PCR can simplify the DNA analysis process by eliminating the extraction, purification and quantitation steps. Presently, the FBI Quality Assurance Standard 9.4 requires all evidence samples to be quantitated. As a result, the direct PCR method of DNA processing cannot be implemented on evidence samples at this time. Direct PCR presents the opportunity to provide efficient DNA analysis, …

Sacramental Ethnicity: Women’S Culture And Vernacular Religion In Twentieth-Century America, Aaron J. Rovan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This project examines the reciprocal and evolving relationship between American women’s culture, vernacular religion, and the social development of American ethnicity. This project focuses on the roles of white ethnic women, both literary and real, in the construction, maintenance, and transmission of ethnic identity. The project highlights the connections between the folkloric performances of vernacular religion and the discursive articulation of ethnicity by focusing on two women writers and two groups of Slovak American women. The fiction of Kate Chopin and Anzia Yezierska illustrates how literary authors bring their contemporary concepts of folklore into their writing. The writings of these …

Application Of Artificial Intelligence For Co2 Storage In Saline Aquifer (Smart Proxy For Snap-Shot In Time), Marwan Mohammed Alnuaimi

Graduate Theses, Dissertations, and Problem Reports (ETD)

In recent years, artificial intelligence (AI) and machine learning (ML) technology have grown in popularity. Smart Proxy Models (SPM) are AI/ML based data-driven models which have proven to be quite crucial in petroleum engineering domain with abundant data, or operations in which large surface/ subsurface volume of data is generated. Climate change mitigation is one application of such technology to simulate and monitor CO2 injection into underground formations.

The goal of the SPM developed in this study is to replicate the results (in terms of pressure and saturation outputs) of the numerical reservoir simulation model (CMG) for CO2 injection into …

Capillary Electrophoresis: Method Development And Application In Biomolecule Analysis, Lindsay Marie Veltri

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Capillary Electrophoresis: Method Development and Application in Biomolecule Analysis

Lindsay Veltri

Biomolecule analysis is essential in disease diagnostics and the development of therapeutic agents. There is a vast array of methods available for biomolecule analysis, however, capillary electrophoresis is well-suited due to minimal sample consumption (~nanoliters of solution) and fast analysis times. In this report, capillary electrophoresis was used to analyze glycosylation of therapeutic proteins. Additionally, a capillary electrophoresis-mass spectrometry method is reported for the analysis of proteins.

Post-translation protein modifications like glycosylation, play an important in protein structure and function. Modifications of protein glycosylation can lead to improved …

Combinatorial Approaches For Effective Design, Synthesis, And Optimization Of Enzyme-Based Conjugates, Jordan Scott Chapman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The specificity and efficiency with which enzymes catalyze selective chemical reactions far exceeds the performance of traditional heterogeneous catalysts that are predominant in industrial applications such as conversion of commodity chemicals to value-added products, fuel cells, and petroleum refinement. Moreover, biocatalysts exhibit exceptionally high product turnover at ambient conditions with little health and environmental burden. These advantageous qualities have led to the prolific use of enzyme catalysis in pharmaceutical, detergents, and food preservation industries wherein their use has greatly reduced waste generation, Unfortunately, the full slate of benefits that enzymes can impart to a broader range of chemical processes is …

Syntheses Of Indoles And Natural Indole Alkaloids From 2-Nitrostyrene Derivatives, S M Ashikur Rahman

Graduate Theses, Dissertations, and Problem Reports (ETD)

A short and flexible synthesis of 2,3-disubstituted indoles employing a Barluenga-type palladium-catalyzed cross-coupling of p-tosylhydrazones with 2-nitroarylhalides followed by a palladium-catalyzed, carbon monoxide-mediated reductive cyclization was developed. Significantly better overall yields were obtained performing the two-step reaction sequence in a one-pot reaction. Secondly, in order to facilitate biological studies of ergotryptamine, a recently isolated ergot alkaloid, it was selected as a target for synthesis. In an attempt to synthesize ergotryptamine, a palladium catalyzed Kosugi-Migita-Stille cross coupling, a Mizoroki-Heck reaction, and a carbon monoxide-mediated palladium-catalyzed reductive cyclization were utilized as the key steps. Using a similar synthetic approach, synthesis of …

Face Recognition With Attention Mechanisms, Qiangchang Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Face recognition has been widely used in people’s daily lives due to its contactless process and high accuracy. Existing works can be divided into two categories: global and local approaches. The mainstream global approaches usually extract features on whole faces. However, global faces tend to suffer from dramatic appearance changes under the scenarios of large pose variations, heavy occlusions, and so on. On the other hand, since some local patches may remain similar, they can play an important role in such scenarios. Existing local approaches mainly rely on cropping local patches around facial landmarks and then extracting corresponding local representations. …

Methodology For Enhanced Filtration And Dewatering Of Fine-Grain Amd Precipitate Using Geocomposites For Rare Earth Elements Recovery, Iuri Lira Santos

Graduate Theses, Dissertations, and Problem Reports (ETD)

Research performed by the West Virginia Water Research Institute (WVWRI) in partnership with the Department of Energy (DOE) has identified that coal mining by-products contain significant amounts of Rare Earth Elements (REEs). REEs currently are in high demand, and extraction from coal mining reject presents a potential for commercialization if extraction is feasible. The coal mining reject include Acid Mine Drainage (AMD) in the aqueous phase and sludge from AMD treatment. A novel two split selective precipitation method was implemented to remove gangue metals, increasing the concentration of REEs in the sludge.

This process presents a two-fold benefit: 1) treatment …

Development Of A Thermal Desalination System Using Low Quality Thermal Energy, Takudzwa C. Chipunza

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Development of a Thermal Desalination System Using Low Quality Thermal Energy

Takudzwa Chipunza

The blowdown water from the cooling tower of the power plant and the produced water from shale gas industry cannot be disposed directly to the environment as they may contain dissolved solids that are harmful to the environment. A detailed physical and chemical process to remove the mineral and organic solids has been developed in West Virginia University. Such a process includes running the blowdown and produced water through a softening tank, activated carbon tank, and a reverse osmosis (RO) system. The RO reject contains a …

Nigerian Teachers’ Perceptions And Experiences Of Integrating Literacy Strategies Into Science Instruction, Joy Oghogho Isa

Walden Dissertations and Doctoral Studies

Reading problems among students in Nigeria prompted the implementation of a literacy across the curriculum initiative in a cluster of schools within a district, where all teachers were required to integrate literacy strategies into their lessons. The problem investigated in this study was that the teachers’ instruction of science at Beta Schools had not produced the expected results with respect to integrating literacy strategies into the science lessons. The purpose of this basic qualitative study was to understand the teachers experiences and perceptions of literacy integration in science lessons. Grounded in the content area literacy conceptual framework, the research questions …

Risk Factors Of Infant Mortality Among Sub-Saharan Immigrants In The United States, Roland Lankah

Walden Dissertations and Doctoral Studies

While prepregnancy body mass index (BMI), gestational weight gain (GWG), and birthplace have been suggested to play a key role in predicting infant mortality outcomes, they have not been thoroughly investigated across all races/ethnicities. The purpose of this study was to examine the associations between prepregnancy BMI, GWG, birthplace as independent variables, and infant mortality as the outcome variable within the United States Sub-Saharan African Immigrants (SSAI). A quantitative cross-sectional study design grounded in the biopsychosocial model was used. Data were extracted from the 2017-2018 natality-linked birth/infant death file from the Centers for Disease Control and Prevention Wonder Database. It …

Special Education Challenges In Transitioning To Online Learning In Wyoming During The Covid-19 Pandemic, Elizabeth Merritt

Walden Dissertations and Doctoral Studies

When the COVID-19 pandemic occurred, education had unexpected impacts, including the closure of schools and the disruption of learning for students. Students with disabilities experienced increased challenges related to these impacts. The problem was to understand the challenges of secondary special education teachers and parents to meet the individualized needs of students with disabilities when transitioning from in-classroom to online learning during the COVID-19 pandemic in Wyoming. The purpose of this basic qualitative study was to examine the challenges of secondary special education teachers and parents during the transition from in-classroom to online learning for students with disabilities and identify …

Experiences Of Individuals With Opioid Use Disorder In Medication Assisted Treatment Programs, Lynda M. Maxfield

Walden Dissertations and Doctoral Studies

AbstractOpioid use accounts for many deaths each day in the United States. Although research has shown that retention in medication assisted treatment (MAT) programs increases quality of life for those individuals with opioid use disorder (OUD) and decreases premature death, there has been limited research on the perspectives of individuals with OUD in MAT programs and why they remained in or left the programs. The purpose of this qualitative phenomenological study, guided by Merleau-Ponty’s theory of perception, was to explore the lived experiences of those with OUD treated in a MAT program. An algorithm type interview was conducted to ask …

The Experience And Perception Of Contraceptive Use Among Teenage Girls Living In Lagos, Nigeria, Kikelomo Omotoso

Walden Dissertations and Doctoral Studies

AbstractThere is a low level of contraceptive use among Nigerian teenagers, leading to unwanted pregnancies and subsequently unsafe abortions. Sexual activities among teenagers in Lagos, Nigeria, are a major public health problem. The principal factors leading to this health problem are poverty, peer pressure among university students leading to involvement in the sex trade, and early marriage in some cultures. There are myths and perceptions about the use of contraception in society, especially concerning the use of condoms, which Nigerian teenagers believe do not make intercourse interesting or satisfying; these ideas can lead to unwanted pregnancies, sexually transmitted infections (STIs), …

Role Of Hypertension In Low-Birth-Weight Racial Disparities Among Virginian Women, Ashli Owen

Walden Dissertations and Doctoral Studies

Low birth weight (LBW) is a leading cause of infant mortality globally, but it disproportionately burdens the Black community in the United States, with a rate twice that of White women. Further, hypertensive disorders of pregnancy (HDsP) increase LBW incidence. The purpose of the study was to assess the association between HDsP and LBW, maternal race and LBW, and the moderation effect of HDsP on race and LBW on Black and White women in Virginia. The theoretical framework for this study was based on the lifecourse health development model, which states that health should be viewed as an outcome of …

The Experiences Of Female Survivors Of Sexual Assault When They Make A Police Report, Tracy L. Rainey

Walden Dissertations and Doctoral Studies

Nearly 20% of all women will be the victim of an attempted or completed sexual assault within the United States, yet less than one quarter of victims will report their experienced crime to law enforcement (LE). Many survivors of sexual violence experience apprehension about reporting sexual assault, due to fear of social consequences, relational consequences, and fear of the reprisal from LE officers. The purpose of this interpretive descriptive qualitative study was to describe and interpret the experiences of adult, female sexual assault survivors when they make a LE report about their victimization. Resilience theory, which is the study of …

The Effectiveness Of Culturally Specific Interventions On Young Adult Latina Women, Cheryl Denise Smith

Walden Dissertations and Doctoral Studies

Using culturally specific interventions to provide mental health support to young adult Latina women may improve mental healthcare results by furnishing resources that are culturally competent and inclusive. Young adult Latina women continue to experience disparities with mental healthcare resources such as cultural adaptability and accessibility. The purpose of this phenomenological qualitative study was to explore how culturally specific interventions could result in mental healthcare support for young adult Latina women who are experiencing mental health issues such as depression and anxiety. Bronfenbrenner's ecological system theory was used as the conceptual framework. Purposeful sampling was used to recruit 12 young …

Barriers To The Implementation Of Effective Sustainable Development Plans In Sierra Leone: Stakeholders’ Perspectives, Mohamed Nabie Turay

Walden Dissertations and Doctoral Studies

Abstract:Stakeholders’ competing positions with elected officials created barriers to the effective implementation of sustainable environmental policies. This study aimed to explore the barriers to implementing the National Environmental Policies (NEP), passed by the parliament of Sierra Leone in the 1990s. Sabastier and Jenkin-Smith’s advocacy coalition framework model provided the theoretical foundation for this study. The key research question explored is stakeholders’ perceptions of the barriers to effectively building coalitions to implement sustainable development policies like the NEP in Sierra Leone. A case study research approach was used to understand the barriers to implementing the NEP in Sierra Leone. Data were …

Factors Influencing Human Papillomavirus Vaccination Among Hispanic Youth In Bexar County, Texas, Timothy Edward Wallum

Walden Dissertations and Doctoral Studies

A high number of Hispanic youth in Bexar County, Texas, face morbidity and mortality related to human papillomavirus (HPV) infection due to inadequate vaccination coverage youth. A better understanding of the factors that influence vaccination might help public health authorities to increase coverage. The aim of this quantitative, cross-sectional study, which was based on the socioecological model and involved analysis of weighted survey data from the 2016 to 2018 National Immunization Survey-Teen, was to better understand the multilevel factors influencing HPV vaccine use among Hispanic youth. The weighted sample was representative of the study population in terms of survey characteristics …

Human Capital Management Strategies To Sustain Profitability Of Private Sector Companies In Jamaica, Peta Gay Nadia Waugh

Walden Dissertations and Doctoral Studies

Eighty-two percent of Jamaican workers are employed by micro, small, and medium-sized enterprises, but only 5.1% of private sector entrepreneurial endeavors remain open after 42 months of operations. This low survival rate can inhibit the ability of Jamaican business owners to support their families and provide goods and services to benefit local communities. Grounded in human capital theory, general systems theory, and social change theory, the purpose of this qualitative descriptive multiple case study was to explore strategies CHROs used to sustain the profitability of their private sector companies. Participants comprised three private-sector CHROs officers and three human resource officers …

Childhood Anxiety And Roles Of Parents, Educators, And Pediatric Healthcare Providers In Addressing Protective Factors, Gabriela Zamora-Ahlstrom

Walden Dissertations and Doctoral Studies

AbstractChildhood anxiety affects over 32% of youth in the United States, and when left untreated, contributes to poor school performance, social difficulties, truancy, school dropout rates, juvenile delinquency, substance abuse, and family and community difficulties. Preventing childhood anxiety by implementing effective protective factors for children can eliminate many of these outcomes. Using the biopsychosocial theory and social work systems theories, this study involved exploring the beliefs and attitudes of parents, educators, and pediatric healthcare providers in an affluent suburban community of Detroit (Rochester/Rochester Hills, MI), regarding their roles in providing protective factors against childhood anxiety. A generic qualitative research design …

An Exploration Of The Contribution Of Embodied, Situated Research Strategies To Cultural Ecosystem Services And Landscape Assessment Frameworks: An Environmental Empathy Case Study, Klara Łucznik, Joane V. Serrano, John Martin

Professional Services

Since the Millennium Ecosystem Assessment in 2005, interest has increased in cultural ecosystem services (CESs) research to understand the complexity of the non-material benefits that people obtain from ecosystems. The intangible and interactive characteris-tics of CESs present many challenges regarding how to approach, quantify and even de-fine CESs. In this paper, we suggest looking at CESs through the lens of embodied and situated cognition theories. We advocate that such an approach should be applied to the development stage of CES research projects, as the embodied and situated experience of the site aids the development of research questions and future interventions. …

Air Pollution As Public Nuisance: Comparing Modern-Day Greenhouse Gas Abatement With Nineteenth-Century Smoke Abatement, Kate Markey

Michigan Law Review

Public nuisance allows plaintiffs to sue actors in tort for causing environmental harm that disrupts the public’s use and enjoyment of the land. In recent years, state and local governments have filed public nuisance actions against oil companies, hoping to hold them responsible for the harm of climate change. Since no plaintiff has prevailed on the merits so far, whether these lawsuits are worth bringing, given the other legal avenues available, remains an open question. This Comment situates these actions in their appropriate historical context to show that these lawsuits are neither unprecedented nor futile. In particular, it examines the …

A Scenography Of Inscriptions And Intangibles In The Birthing Room, Johara Bellali

School of Art, Design and Architecture

Inscriptions on graph paper, on monitors and on various screensare ubiquitous in the birthing room; they reassure, worry, beep,light up and inform protocols. According to Latour (1985) theirpresence is not a result of a carefully planned logical choice, butof a legacy of mustering allies and convincing alignments. UsingBarad’s (2007) framework of agential realism, this paper explores thenotion of intra-actions between objects and agencies of observationin the phenomenon of birth, using a socio-political and historicalconfiguration. It presents some surprising accounts of obstetricsand gynaecology and showcases how historical inscriptions andinstruments materialized today’s birthing practices. It then presentshow, upon entering the birthing room, …

A Review Of Thermal Impact Of Surface Acoustic Waves On Microlitre Droplets In Medical Applications, Mubbashar Mehmood, Umar F. Khan, Ali O.M. Maka, Javed Akhter, Tariq Nawaz Chaudhary, Faisal Masood, Sameer Ahmad Hasan, Yeaw Chu Lee

School of Engineering, Computing and Mathematics

The surface acoustic waves (SAW) propagate inside the microdroplets resulting in kinetic and thermal impacts. The kinetic drives fluid particles inside the droplet while thermal impact increases the liquid’s temperature. This paper provides a comprehensive review of the research investigations related to internal kinetics and heating inside the microdroplet caused by the acoustic waves. The main factors that affect the kinetics and convection heat transfer are the piezoelectric materials, shape of the interdigital transducer (IDT) and mode of acoustic waves. Internal streaming (kinetic) leads to particle mixing, particle manipulation, cell sorting, cell patterning, cell separation, measuring the concentration of immunoglobulin …

Ocean Science Diplomacy - The All-Atlantic Ocean Research Alliance Case, Andrei Polejack

World Maritime University Ph.D. Dissertations

Science diplomacy, the interlinkage between research and foreign affairs, is a recent field of research, albeit being claimed to be an ancient practice in international relations. Science diplomacy practices generally include the influence of scientific advice during international negotiations, the bridging of communities (or even conflicting nations) through joint research projects and the promotion of science by diplomatic channels, just to name a few. Largely identified today by mainstream intergovernmental policy-makers as a beneficial practice, science diplomacy is promoted as being rooted in the universality and cooperative nature of science and as a means for peaceful and uncontested international interactions. …

Negotiating The Bbnj Agreement : Exploring The Caribbean Community's Engagement From A Blue Economy Perspective With Special Focus On Environmental Impact Assessment Provisions, Kahlil Hassanali

World Maritime University Ph.D. Dissertations

The countries of the world are engaged in negotiations to develop an international legally binding instrument under the United Nations Convention on the Law of the Sea (UNCLOS) on the conservation and sustainable use of marine biological diversity of areas beyond national jurisdiction (The BBNJ agreement). In the BBNJ process, the member States of the Caribbean Community (CARICOM) participate as a regional bloc and have been actively involved in the negotiations. This thesis applies an action research methodology along with a mix of methods, including the reading of primary, secondary and grey literature; document content analysis; interviews and informal conversations; …

Cancer Testis Antigen Pasd1 Expression And Immunogenicity In Human Colorectal Cancer And Polyps, Nur Fazilah Sukor, Azyani Yahaya, Ismail Sagap, Rahman Jamal, Nor Adzimah Johdi

Turkish Journal of Biology

Colorectal cancer (CRC) is a malignant tumor arising from a human inner colon lining that may spread to other organs such as the liver and lungs. Per ARNT Sim domain containing 1 (PASD1) is a cancer-testis antigen expressed in cancers including CRC but not in normal tissues except for normal testes. This study aims to study PASD1 protein as a potential target for CRC immunotherapy. A total of 90 CRC and polyps tissue samples were investigated for PASD1 RNA and protein expression using a real-time polymerase chain reaction and immunohistochemical staining, respectively. Matched patients? peripheral blood mononuclear cells were pulsed …